KR101236212B1 - Cosmetic composition containing citrus natsudaidai extract for improving skin whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Citrus natsdaidai extract is provided to suppress melanogenesis and tyrosinase and to enhance skin whitening. CONSTITUTION: A cosmetic composition containing a Citrus natsdaidai extract for skin whitening contains: 100 parts by weight of purified water, 10-15 parts by weight of glycerin, 6-8 parts by weight of butylene glycol, 8-10 parts by weight of a hyaluronic acid extract, 12-15 parts by weight of beta-glucan, 14-16 parts by weight of caprylic/capric triglyceride, 8-10 parts by weight of squalene, 2.5-3 parts by weight of cetearyl glucoside, 0.5-1 parts by weight of sorbitan stearate, and 0.15-0.2 parts by weight of triethanol amine. The Citrus natsdaidai extract is a crude extract or a non-polar solvent fraction or a polar solvent fraction of the crude extract. The cosmetic composition is manufactured in the form of a lotion, a skin softener, a skin toner, an astringent, a cream, a foundation, an essence, a pack, a soap, a cleansing foam, or a body cleanser.

Description

Cosmetic composition for skin whitening containing a mandarin orange extract as an active ingredient {Cosmetic composition containing Citrus natsudaidai extract for improving skin whitening}

The present invention relates to a cosmetic composition for skin whitening containing a mandarin orange extract as an active ingredient, and more particularly, containing a mandarin orange extract as an active ingredient can be used safely without side effects on the skin, and also inhibits melanin production The present invention relates to a cosmetic composition for skin whitening that is excellent in inhibiting pigmentation such as freckles and skin whitening effect.

The present invention relates to a cosmetic composition for skin whitening containing a mandarin orange extract as an active ingredient, and more particularly, containing a mandarin orange extract as an active ingredient can be used safely without side effects on the skin, and also inhibits melanin production The present invention relates to a cosmetic composition for skin whitening that is excellent in inhibiting pigmentation such as freckles and skin whitening effect.

Skin is an important tissue that protects the human body while being in direct contact with the external environment and exhibits biochemical and physical functions. This tissue is composed of three major parts: epidermids, dermis and subcutaneous tissue (hgypodermis), and mainly serves as a barrier to protect the human body. Melanin is a phenolic polymer that is widely distributed in nature and is an important pigment that determines the color of the skin and is present in the epidermal layer of the skin. There are mainly eumelanin (black or brown) and pheomelanin (yellow or red). Melanin is made from melanocytes in the basal layer of the epidermis. The synthesized melanin is removed by keratinocyte keratinocyte process as it moves to adjacent epidermal cells called keratinocytes.

Melanin, which determines the color of human skin, is a phenolic polymer that is present in animals, plants, and microorganisms. It is a type of pigment that has a protective function against external environments such as ultraviolet rays, dryness, and extreme temperatures. It is biosynthesized by the continuous oxidation of tyrosinase enzyme in the melanocytes (melanosome) of the melanocytes present.

However, in the skin exposed to sunlight, tyrosinase promotes melanogenesis by preventing the damage of cells and tissues in the skin by ultraviolet light and the progress of photoaging by ultraviolet light. It will form blemishes, freckles, and black mushrooms. This melanin is mainly tyrosine is oxidized to L-DOPA and dopaquinone by tyrosinase and again 5,6-dihydroxyindole, indole-5,6- Biosynthesized by oxidizing to quinone (indol-5,6-quinone) and finally polymerizing (Pawelk JM and Korner AM, Am. Sci., 70, p136, 1982; Butt VS, Recent Adv. Phytochemistry, 12 , p433, 1979; Lopez JNR, J. Tudela, R. Varon, FG Carmona, FG Canovas, J. Biol. Chem., 267, p3901-3910, 1992). That is, the biosynthesis of melanin can be suppressed through prevention of UV exposure of the skin, inhibition of tyrosinase activity, reduction and decolorization of the produced melanin, promotion of release to the outside of the skin, metabolism and inhibition of proliferation of melanocytes. Can be. It has been found that there is a new melanin biosynthesis pathway associated with skin whitening and several new regulators such as TRP-1, TRP-2 and MSH have been found in addition to tyrosinase, but the exact metabolism has not been identified. Because of this condition, the research on the whitening effect through the activity regulation of tyrosinase has been the most active (Slominski A, Tobin DJ, Shibahara S, Wortsman J, Physiol Rev, 84 (4), p1155-1228, 2004). ; Land EJ, Ramsden CA, Riley PA, Mthods Enzymol, 378, p88-109, 2004; Kameyama K, Jimenez M, Muller J, Ishida Y, Hearing VJ, Differentiation, 42 (12), p28-36, 1989; Sturm RA, Melanoma Res, 12 (5), p405-416, 2002).

Human skin color is determined by a number of factors, including the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of the skin and the presence or absence of pigments in the body, such as carotenoids and bilirubin. The most important is a black pigment called melanin produced by the action of various enzymes such as cinnases. The formation of melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion, stress, and environmental factors such as UV irradiation. Melanin exists in the epidermal layer of the skin's outer skin to protect skin organs under the dermis from ultraviolet rays and to protect free radicals generated in the skin's living body. It has important functions to protect proteins and genes in the skin. By overproduction, it is known not only to form spots and freckles, but also to promote skin aging and to play an important role in causing skin cancer.

Recently, women in the Asian region prefer white and clean skin like white jade and make it an important mood for beauty. Therefore, the development of whitening agents for the treatment of skin pigmentation abnormalities and the needs of beauty is actively being made. In the development of a whitening agent, a method of reducing the resulting melanin pigment to decolorize and a method of inhibiting tyrosinase activity, which is an enzyme forming melanin pigment, are known. However, it is known that the whitening agent using tocopherol or vitamins used for reducing the melanin pigment has a very small discoloring effect of the melanin pigment. Therefore, inhibitors that inhibit the production of melanin pigment by inhibiting the activity of tyrosinase have attracted attention. In particular, plant natural products are excellent in safety and have various beneficial ingredients as compared to synthetic raw materials, and their image is good, making them a good search target for cosmetic raw materials.

Citrus natsudaidai originates from summer summer and summer eats fruit and is called tangerine. The mandarin oranges bloom in April and are harvested in May. It is good for admiration because you can watch the fruit all year round. The fruit size is slightly larger than Hallabong, broad and hard without peaks. The fruit is ripe in summer and tastes good.

Although it is native to India, it is said to originate from live animals in Yamaguchi Prefecture, Japan in the 1700s. It is larger than citron, and its yellow shell is thick and its acidity is strong enough to close your eyes when you eat it. Therefore, it is used as a tea ingredient rather than just eating. The mandarin is known as a medicine that controls and strengthens the heart and small intestine. The peel of the tangerine has a spicy taste, which is stronger and stronger than citrus peel, and is used as a medicine to protect the stomach.

The rind contains essential oil limonene, bitter ingredient narigin, and hesperidin, a cause of turbidity in the extraction of juice, and a small amount of aldehydes, alcohols, esters, terpenes, and the like. It also contains vitamins A and B, especially vitamin C. In addition, the skin contains hesperidin, which is a flavone-based vitamin P that protects the capillaries. The herbaceous wood is described as the skin to digest and improve the inside of the stomach, and the medicinal metabolism is digested and, in the case of food poisoning, it has the effect of detoxifying it. Citrus fruits are rich in vitamins and various organic acids, as well as limonoids, which are known for their excellent anticancer effects, and hesperidin, which is known for their effects on liver detoxification and liver detoxification, anticancer, anti-inflammatory, and antibacterial effects. This is known to be abundant. Pigment pigments contain immature chlorophyll and carotenoids and mature ones contain carotene, lycopene and xanthophylls (Journal of Cardiovascular Pharmacology 1993 Aug; 22 (2): 225-30)

On the other hand, an example of the preceding invention regarding the mandarin orange extract, immature citrus extract whitening composition (Korea Patent 10-2006-0104054), immature citrus essential oil extract having anti-inflammatory activity (Korea Patent 10-2007-0062002), Jeju natural tangerine Although there are patents for citrus extracts such as fragrance compositions and cosmetic compositions using the same (Korean Patent 10-20090087725) as an active ingredient, there is no disclosure or teaching about the composition containing the mandarin extract having a whitening effect on the skin. .

Accordingly, the present inventors completed the present invention by confirming that the tangerine extract has an excellent whitening effect while identifying substances inhibiting tyrosanase activity and melanogenesis in relation to skin whitening in natural products.

An object of the present invention is to provide a cosmetic composition for skin whitening containing a mandarin extract excellent in tyrosinase inhibitory activity and melanin production inhibitory effect, excellent skin whitening effect, a small number of skin irritation as an active ingredient will be.

An object of the present invention is achieved by providing a cosmetic composition for skin whitening containing a tangerine extract having an inhibitory effect on tyrosinase activity and melanogenesis production as an active ingredient.

According to a preferred feature of the present invention, the mandarin extract is to be contained 0.001 to 20 parts by weight relative to 100 parts by weight of the cosmetic composition for skin whitening containing the mandarin extract as an active ingredient.

According to a more preferred feature of the invention, the cosmetic composition for skin whitening containing the mandarin extract as an active ingredient purified water, glycerin, butylene glycol, hyaluronic acid extract, beta glucan, chamomer, mandarin extract, caprylic / capric Trigly cerad, squalane, cetearyl glucoside, sorbitan stearate, cetearyl alcohol and triethanolamine are assumed.

According to a more preferable feature of the invention, the cosmetic composition for skin whitening containing the mandarin extract as an active ingredient 100 parts by weight of purified water, 10 to 15 parts by weight of glycerin, 6 to 8 parts by weight of butylene glycol, 8 to 10 of hyaluronic acid extract Parts by weight, beta-glucan 12 to 15 parts by weight, 0.15 to 0.2 parts by weight of chamoner, 0.01 to 20 parts by weight of the mandarin extract, 14 to 16 parts by weight of caprylic / capric trigly cerad, 8 to 10 parts by weight of squalane, three Tearyl glucoside 2.5 to 3 parts by weight, sorbitan stearate 0.5 to 1 part by weight and triethanolamine 0.15 to 0.2 parts by weight.

According to a further preferred feature of the present invention, the mandarin orange extract is composed of one or more extracts selected from the group consisting of crude extract of mandarin orange, nonpolar solvent fraction of mandarin orange crude extract or polar solvent fraction of mandarin orange crude extract.

According to a further preferred feature of the invention, the crude extract of the mandarin is one or more solvents from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, glycerin, ether and chloroform Shall be obtained by extraction.

According to a further preferred feature of the invention, the non-polar solvent fraction of the mandarin crude extract is a non-polar solvent selected from the group consisting of hexane, chloroform, methylene chloride, ethyl acetate, glycerin and butylene glycol It shall be obtained by fractionation.

According to a further preferred feature of the present invention, the polar solvent fraction of the crude mandarin extract is obtained by fractionating the crude extract of the mandarin with one polar solvent selected from the group consisting of water and n-butanol.

According to a further preferred feature of the present invention, the cosmetic composition for skin whitening containing the mandarin extract as an active ingredient, lotion, skin softener, skin toner, astringent, cream, foundation, essence, pack, soap, cleansing foam And it is to be prepared in one formulation selected from the group consisting of a body cleanser.

The cosmetic composition for skin whitening containing the mandarin orange extract according to the present invention contains a mandarin extract having excellent tyrosinase inhibitory activity and melanin production inhibitory effect, and has an excellent skin whitening effect and less skin irritation. Indicates.

Hereinafter, preferred embodiments of the present invention and physical properties of the respective components will be described in detail with reference to the accompanying drawings. However, the present invention is not limited thereto, And this does not mean that the technical idea and scope of the present invention are limited.

The cosmetic composition for skin whitening containing the tangerine extract according to the present invention is made by containing a tangerine extract having an inhibitory effect on tyrosinase activity and inhibiting melanogenesis as an active ingredient, and the tangerine extract is an effective tangerine extract It is preferable that 0.001-20 weight part is contained with respect to 100 weight part of skin cosmetic compositions containing as a component.

The mandarin extract contains 0.001 to 20 parts by weight based on 100 parts by weight of the cosmetic composition for skin whitening containing the mandarin extract as an active ingredient, crude extract of mandarin, nonpolar solvent fraction of mandarin crude extract or mandarin crude extract At least one extract selected from the group consisting of polar solvent fractions.

The crude extract of the mandarin is obtained by extracting the mandarin with one or more solvents from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, glycerin, ether and chloroform, and the mandarin crude extract The non-polar solvent fraction of is obtained by fractionating the crude extract of the mandarin with one non-polar solvent selected from the group consisting of hexane, chloroform, methylene chloride, ethyl acetate, glycerin and butylene glycol, the polar solvent fraction of the crude orange extract The crude extract of the mandarin is obtained by fractionation with one polar solvent selected from the group consisting of water and n-butanol.

Looking at the process of obtaining the mandarin crude extract, after separating and pulverizing the flesh or skin of the mandarin as water, the lower alcohol or propylene glycol having 1 to 4 carbon atoms, ethyl acetate, butylene glycol water, ether, chloroform 1 to 30 times, preferably 5 to 25 times, more preferably 10 to 20 extracts available in at least one solvent selected from the group consisting of water, preferably water, ethanol, methanol, butylene glycol, propylene glycol or glycerin In the state of preventing the evaporation of the solvent by adding the volume of the double volume at room temperature or with a cooling condenser, extraction is performed by extraction methods such as stirring extraction, cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, and heating extraction. 1 to 7 times by stirring or extracting by heating and filtration and depositing for 1 to 15 days at 1 to 10 ℃, preferably It may afford the hagyul crude extract of the present invention was concentrated under reduced pressure and dried after extracting three times to.

In addition, the non-polar solvent fraction of the crude mandarin extract and the polar solvent fraction of the crude mandarin extract are 2 to 30 times, preferably 8 to 25 times the weight of the ethanol-soluble crude extract of the mandarin crude extract obtained above, preferably mandarin orange. More preferably, 10 to 20 times the volume of water is dispersed, and then nonpolar solvents such as hexane, ethyl acetate, chloroform and polar solvents such as butanol and ethanol are sequentially added in the same volume with water, 1 to 5 times, preferably Preferably it can be fractionated 2 to 4 times to obtain the mandarin nonpolar solvent and the polar solvent soluble extract of the present invention.

In addition, the crude mandarin extract, the nonpolar solvent fraction of the mandarin crude extract and the polar solvent fraction of the tangerine extract exhibited excellent tyrosinase inhibitory activity and melanogenesis inhibitory effect, and also stably because there is little irritation when applied to the skin. Can be used.

In addition, the cosmetic composition for skin whitening containing the mandarin orange extract according to the present invention is purified water, glycerin, butylene glycol, hyaluronic acid extract, beta glucan, chamomer, mandarin orange extract, caprylic / capric trigly cerad, It consists of squalane, cetearyl glucoside, sorbitan stearate, cetearyl alcohol and triethanolamine, 100 parts by weight of purified water, 10 to 15 parts by weight of glycerin, 6 to 8 parts by weight of butylene glycol, 8 to 10 parts by weight of hyaluronic acid extract. 12, 15 parts by weight of beta glucan, 0.15 to 0.2 parts by weight of chamoner, 0.01 to 20 parts by weight of mandarin extract, 14 to 16 parts by weight of caprylic / capric trigly cerad, 8 to 10 parts by weight of squalane, cetea 2.5 to 3 parts by weight of reel glucoside, 0.5 to 1 part by weight of sorbitan stearate and 0.15 to 0.2 part by weight of triethanolamine It is preferred.

At this time, the mandarin extract may be used in a variety of cosmetics and cleansing agents having a skin whitening effect, the formulation of the skin whitening agent composition containing the mandarin extract lotion, skin softener, skin toner, astringent, cream, foundation, essence , Pack, soap, cleansing foam, and body cleanser.

More specifically, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing It may consist of foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the above-mentioned formulations are paste, cream or gel type, the carrier component contains animal fibers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide. do.

When the above-mentioned formulations are powder or spray type, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray type, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

If the formulations listed above are of solution or emulsion type, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

If the formulations listed above are of suspension type, the carrier component may be water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like.

When the above-mentioned formulation is a surfactant-containing cleansing type, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The formulation also contains one or more components selected from the group consisting of water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be blended in cosmetics, any one can be used, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, Vitamin C, vitamin H, and the like, and salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) are also used. It is possible and can be obtained through conventional methods such as transformation, purification from culture of microorganisms, enzyme or chemical synthesis.

The oil-soluble vitamin is not particularly limited as long as it can be formulated in cosmetics, any one can be used, preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d- Alpha tocopherol); and derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid, ascorbic acid, dl-alpha tocopherol acetate, dl-alpha tocopherol vitamin E, DL-pantothenyl alcohol, D) -Pantothenyl alcohol, pantothenyl ethyl ether, etc.) can also be used, and can be obtained through conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide is not particularly limited as long as it can be blended into cosmetics, and any one can be used. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be used. It can be obtained through conventional methods such as purification method, enzyme method or chemical synthesis method, and can also be obtained by purification from natural products such as dermis such as pigs and cows and silk fibers of silkworms.

The polymer polysaccharide is not particularly limited as long as it can be blended into cosmetics, and any one can be used. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be used. Can be. For example, chondroitin sulfate or a salt thereof may be obtained through a purification process from a mammal or a fish.

The sphingolipid is not particularly limited as long as it can be blended into cosmetics, and any one can be used. Preferably, ceramide, phytosphingosine, sphingolipid, and the like can be used. Sphingo lipids are usually purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract is not particularly limited as long as it can be blended into cosmetics, and any one can be used, and preferably, brown algae extract, red algae extract and green algae extract can be used. Arginic acid, sodium arginate, potassium arginate and the like can also be used.

Seaweed extract can be obtained from seaweed through the process of purification by conventional methods.

In addition, the cosmetic composition of the present invention may further include a component that is usually blended into the cosmetic composition as necessary in addition to the essential components.

The conventional cosmetic formulations include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings. , Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylic acid glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, neopentane dodecyl Isostearyl octanoate, octyl isononate, hexyl decyl neodecanoate, octyl dodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-stealo Esters such as monohydroxystearic acid isostearyl; and the like.

Hydrocarbon-based oils, such as squalane, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and petrolatum, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, In addition, although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, 0.01-5 weight part with respect to 100 weight part of cosmetic compositions for skin whitening. It is preferable to add addition, and it is more preferable that 0.01-3 weight part is added.

In addition, the cosmetic composition for skin whitening according to the present invention may be obtained in the form of a solution, an emulsion, a viscous mixture, and the like, and in addition to the components listed above, components commonly used in cosmetic compositions may be further included. For example, it may further include conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments and flavorings.

Hereinafter, the method of extracting the tangerine extract contained in the cosmetic composition for skin whitening according to the present invention and the tyrosinase inhibitory effect of the skin whitening cosmetic composition containing such a mandarin extract, the melanin production inhibitory effect using melanoma cells, Cell viability using melanoma cells will be described using examples.

In addition, the preparation and effect of the formulation containing the cosmetic composition for skin whitening according to the present invention will be described with reference to Examples.

Example  One. A tangerine Crude extract  Produce

Example 1-1. Cut the mandarin pulp into 1L of 95% (w / w%) ethanol, and extract it by stirring for 5 days at room temperature, filter it with 250 mesh filter cloth, leave it to stand at 4 ℃ for 7 days, and then filter it with Whatman filter paper. Crude crude extract 8.1L (solid content 68g) was obtained.

Example 1-2. Cut the mandarin peel into 1kg in 10% of 95% (w / w%) ethanol, extract the mixture by stirring at room temperature for 5 days, filter it with 250 mesh filter cloth, leave it to stand at 4 ° C for 7 days, and filter it with Whatman filter paper. 8.3L of crude mandarine extract (43.2 g of solid) was obtained.

Example 1-3. Except for changing 95% ethanol to purified water, the same reaction process as in Example 1-1 was carried out to obtain 9.4 L of an extract (59.4 g of solid).

Example 1-4. Except for changing 95% ethanol to purified water, the same reaction process as in Example 1-2 was performed, thereby obtaining 9.5 L of an extract (41.7 g of solid).

Examples 1-5. Except for changing 95% ethanol to 100% methanol, the same reaction process as in Example 1-1 was carried out, thereby obtaining 8.2L of an extract (71.3 g of a solid).

Example 1-6. Except for changing 95% ethanol to 100% methanol, the same reaction process as in Example 1-2 was performed, to obtain 8.9 L of an extract (44.2 g of solid).

Example 1-7. Except for changing 95% ethanol to 30% ethanol, the same reaction process as in Example 1-1 was carried out to obtain 8.9 L of an extract (66.8 g of solid).

Example 1-8. Except for changing 95% ethanol to 30% ethanol, the same reaction process as in Example 1-2 was carried out to obtain 8.8 L of an extract (42.2 g of solid).

Example 1-9. Except for changing 95% ethanol to 70% ethanol, the same reaction process as in Example 1-1 was carried out to obtain an 8.6L extract (67.7 g of solid).

Example 1-10. Except for changing 95% ethanol to 70% ethanol, the same reaction process as in Example 1-2 above was performed, to obtain 8.7 L of an extract (45.1 g of solid).

Example 1-11. Except for changing 95% ethanol to 100% propylene glycol, the same reaction process as in Example 1-1 was carried out to obtain an extract 9.3L (54.8 g of solid).

Example 1-12. The same reaction process as in Example 1-2 was carried out except for changing 95% ethanol to 100% propylene glycol, to obtain 9.1L of an extract (40.9 g of solid).

Example 1-13. Except for changing 95% ethanol to 30% propylene glycol, the same reaction process as in Example 1-1 was carried out to obtain 8.9 L of extract (61.1 g of solid).

Example 1-14. Except for changing 95% ethanol to 30% propylene glycol, the same reaction process as in Example 1-2 was carried out to obtain 9.2 L of an extract (43.8 g of solid).

Example 1-15. The same reaction process as in Example 1-1 was carried out except for changing 95% ethanol to 100% butylene glycol, thereby obtaining 9.2 L of an extract (58.7 g of solid).

Example 1-16. Except for changing 95% ethanol to 100% butylene glycol was carried out in the same reaction process as in Example 1-2 to obtain 9.2L extract (44.6g of solid content).

Example 1-17. Except for changing 95% ethanol to glycerin, the same reaction process as in Example 1-1 was carried out to obtain 9.5 L of extract (60.1 g of solid).

Example 1-18. Except for changing 95% ethanol to glycerin, the same reaction process as in Example 1-2 was carried out to obtain 9.6 L of an extract (38.8 g of solid).

Example  2. A tangerine  Preparation of Nonpolar Solvent Soluble Extract

Example 2-1. 40 g of the extract solid obtained in Example 1-9 was dispersed in 500 ml purified water, and 500 ml of n-hexane was added thereto, shaken for 2 to 3 hours at room temperature, and then divided into an upper layer and a lower layer. Hexane) is recovered. The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 4.2 g of extract powder.

Example 2-2. 30 g of the extract solid obtained in Example 1-10 was dispersed in 500 ml purified water, and 500 ml of n-hexane was added thereto, shaken for 2 to 3 hours at room temperature, and then divided into an upper layer and a lower layer. Hexane) is recovered. The extract obtained by carrying out 3 times in the same manner was concentrated under reduced pressure to obtain 3.5 g of extract powder.

Example 2-3. 500 ml of ethyl acetate was added to the lower layer solution (water layer) obtained in Example 2-1, shaken and left at room temperature for 2 to 3 hours, then divided into an upper layer and a lower layer to recover the supernatant solution (ethyl acetate). The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 9.8 g of extract powder.

Example 2-4. 500 ml of ethyl acetate was added to the lower layer solution (water layer) obtained in Example 2-2, shaken and left for about 2 to 3 hours at room temperature. The mixture was partitioned into an upper layer and a lower layer, and the upper layer solution (ethyl acetate) was recovered. The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 6.2 g of extract powder.

Example  3. A tangerine  Preparation of Polar Solvent Soluble Extract

Example 3-1. 500 ml of n-butanol was added to the lower layer liquid (water layer) obtained in Example 2-3, and the mixture was left to stand at room temperature for 2 to 3 hours, and then divided into an upper layer and a lower layer to recover the supernatant liquid (butanol). The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 10.2 g of extract powder.

Example 3-2. 500 ml of n-butanol was added to the lower layer liquid (water layer) obtained in Example 2-4, and the mixture was left to stand at room temperature for 2 to 3 hours, and then divided into an upper layer and a lower layer to recover the supernatant liquid (butanol). The extract obtained by performing the same method three times was concentrated under reduced pressure to obtain 7.8 g of extract powder.

Example 3-3. The lower layer liquid (water layer) obtained in Example 3-1 was concentrated under reduced pressure to obtain 10.8 g of extract liquid powder.

Example 3-4. The lower layer liquid (water layer) obtained in Example 3-2 was concentrated under reduced pressure to obtain 9.2 g of extract liquid powder.

Experimental Example  One. Tyrosinase  Inhibitory effect

The inhibitory effect of tyrosinase activity on the tangerine crude extracts of Examples 1-9 to 1-10 and the solvent fractions of Examples 2 and 3 was measured as follows using the method described in the literature (A Vanni, Annali di Chimica., 80, p35, 1990) After mixing 0.5 ml of potassium phosphate buffer solution (pH 6.8, 0.1M) with 0.5 ml of L-Tyrosine (0.3 mg / ml) Example 1-9 to 1-10 and prepared by diluting each sample of Example 2 and Example 3 to a certain concentration in the extraction solvent) 0.45ml for each concentration is put at room temperature conditions and maintained at 37 ℃ for 20 minutes. After that, add 0.05 ml of Tyrosinase (5370 units / ml, SIGMA, T-3824) and leave for 10 minutes to proceed with enzyme reaction. Take out within 10 minutes ± 5 seconds and terminate the reaction under frozen conditions consisting of ice water. At this time, the control group used the extraction solvent instead of the sample for each concentration and the blank the same amount of potassium phosphate buffer instead of Tyrosinase. Finally, after confirming the absorbance value by irradiating the UV of 480nm wavelength, the tyrosinase inhibitory effect of the sample for each concentration is obtained by the following formula.

&Quot; (1) "

% Tyrosinase Inhibition = [1-(Enzyme Activity of Plant Extract / Control Enzyme Activity at Each Concentration)] × 100

Tyrosinase inhibitory activity IC ₅₀ was expressed as the concentration of crude extract and solvent fraction (㎍ / ㎖) required for 50% inhibition of tyrosinase activity, the results are shown in Table 1 below, the activity comparison For the skin whitening notice, Arbutin, which is used as a functional ingredient, was used as a control.

TABLE 1

As shown in Table 1 above, it was confirmed that the tangerine crude extract, the nonpolar solvent fraction of the tangerine crude extract, and the polar solvent fraction of the tangerine extract exhibited excellent tyrosinase inhibitory effect.

Experimental Example  2. B16 Melanoma  Inhibitory Effects of Melanogenesis on Cells

The inhibitory effect of melanogenesis of the tangerine crude extracts of Examples 1-9 to 1-10 and the polar solvent fractions of the tangerine crude extracts of Examples 2 and 3 and the polar solvent fraction of the tangerine extracts using B16 melanoma cells The method described in the literature was modified and measured as follows (Maeda, Fukuda, J. Soc. Cosmetic Chem., 42, p361, 1991).

B16 melanoma cells (ATCC CRL6323) were inoculated at a density of 1 × 10 5 cells in DMEM (Dulbecco's modified Eagle's Medium) with 10% bovine serum and 1% antibiotics (5% CO2, 37 ° C, 24 ° C). time). Then, the cells were treated with DMEM (10% bovine serum) medium treated with α-MSH (melanocyte stimulating hormone; melanocyte stimulating hormone, 0.34 µg / ml, Sigma, USA), and the samples were treated in wells by concentration. Incubated daily. After 3 days, the medium was collected in a 1.5 ml tube, cells were removed by trypsin treatment, and the medium was transferred to the collected tube. The supernatant was discarded by centrifugation at 3,000 rpm for 30 minutes, treated with 250 μl of 1N NaOH, and then centrifuged lightly for 10 minutes in boiling water. After 10 minutes of sonication, the melanin was completely dissolved, and the absorbance at 475 nm was measured to measure the amount of melanin produced. All experiments were repeated three times and the average value was used as a statistical process, the results are shown in Table 2 and Table 3, and the arbutin (Arbutin) used as a functional ingredient for skin whitening notice for active comparison was used as a control.

<Table 2>

<Table 3>

As a result of the experiment, as shown in Table 2 and Table 3, the tangerine crude extract, the non-polar solvent fraction of the tangerine crude extract, and the polar solvent fraction of the tangerine extract are almost similar to the arbutin used as a functional ingredient for skin whitening notice. It was confirmed that the excellent melanin production inhibitory effect.

Experimental Example  3. B16 Melanoma  Cell viability measurement using cells

Whether or not the melanogenesis of the mandarin crude extracts of Examples 1-9 to 1-10 and the non-polar solvent fractions of the tangerine crude extracts of Examples 2 and 3 and the polar solvent fraction of the tangerine extract is due to cytotoxicity To determine the cell viability of B16 melanoma cells was measured using the MTT assay described in the literature (Jae Gap Park, Cancer Research Technique, Medical Publication History, p104-117, 2001).

B16 melanoma cells (ATCC CRL6323) were inoculated at a density of 1 × 10 5 cells in DMEM (Dulbecco's modified Eagle's Medium) with 10% bovine serum and 1% antibiotics (5% CO2, 37 ° C, 24 ° C). time). Thereafter, the cells were changed to fresh DMEM (10% bovine serum) medium, and the samples were incubated for 3 days by treating the wells at different concentrations. After 3 days, the medium was removed and 0.33 mg / ml of MTT ((3- [4,5- 1 ml of dimethylthiazole-2-yl] -2,5-diphenyltetrazoliumbromide), Sigma M5655, USA) was treated for 4 hours in a 5% CO2, 37 ℃ incubator. Thereafter, MTT was removed, and 1 ml of DMSO (dimethylsulfoxide) was added to measure absorbance at 575 nm wavelength. All experiments were repeated three times and the average value was used as a statistical treatment, and the results are shown in Tables 4 and 5 below, Arbutin (Arbutin) used as a functional ingredient for skin whitening notice was used as a control.

TABLE 4

<Table 5>

As a result, as shown in Table 4 and Table 5, the mandarin crude extract, the non-polar solvent fraction of the mandarin crude extract and the polar solvent fraction of the mandarin orange extract are B16 melanoma cells at concentrations showing melanogenesis inhibitory effect It was confirmed that no cytotoxicity against.

In addition, the formulation of the cosmetic composition for skin whitening according to the present invention may be arbitrarily selected, and may be prepared in various forms such as soft cosmetics, lotion, nutrition cream, massage cream, essence, etc. which are conventional cosmetic formulations.

Manufacturing example  1. flexible lotion ( skin Manufacturing

The components and contents of the flexible lotion (skin) (Preparation Example 1) and the content of the general flexible lotion (Comparative Example 1) containing the mandarin extract are shown in Table 6 below.

<Table 6>

The flexible lotion of Preparation Example 1 is prepared by dissolving hydrolyzed castor oil, methylparaben, and combination perfume in ethanol, and adding the remaining components to dissolved purified water.

Manufacturing example  2. Preparation of nutrient lotion (lotion)

The components and contents of the nutrient lotion (lotion) containing the mandarin orange extract are shown in Table 7 below.

<Table 7>

The nutritional lotion of Preparation Example 2 is an aqueous phase component at a predetermined temperature (80 to 85 ° C) using a raw material having the above-mentioned content as an oil phase component and a component dissolved in purified water as an oil phase component. It is prepared through the emulsification process of homogeneous mixing of oily ingredients with general homomixer, neutralizing thickener (carbomer), and then cooling, flavoring, adding crude mandarin extract and defoaming.

Manufacturing example  3. Manufacture of nutritional cream

The ingredients and contents of the nutrition cream containing the mandarin orange extract are shown in Table 8 below.

<Table 8>

The water-soluble component and oil-based component at a constant temperature (80 to 85 ° C) are used as a general homomixer, with the ingredients dissolved in oil as the oil phase components and the ingredients dissolved in purified water as the aqueous phase. After the emulsification process of homogeneous mixing, the thickener (carbomer) was neutralized, and then cooled, flavored, added mandarin extract, and defoaming to complete the preparation.

Manufacturing example  4. Preparation of Essence

The components and contents of the essence containing the tangerine extract are shown in Table 9 below.

<Table 9>

Manufacturing example  5. Massage cream  Produce

The ingredients and contents of the massage cream containing the mandarin orange extract are shown in Table 10 below.

<Table 10>

clinical Test Example  1. Safety and human body Patch  exam

In order to determine the degree of skin irritation of the cosmetic according to the present invention, 20 test subjects were subjected to the skin patch test by applying the formulation prepared in Preparation Examples 1 to 5 and the formulation of Comparative Example 1 prepared in the present invention.

A formulation containing no crude tangerine extract was used as a control. 0.2 ml of nutrient lotion of Formulation Examples 1 to 5 and Comparative Example 1 was added to a fin chamber and patched to the upper arm of the human body for 24 hours, and after removing the patch, the skin reaction was examined within 1 hour, and after about 48 hours. It was examined again. Skin response was determined by the test criteria in Table 11, the average response rate was calculated according to the following equation (2).

<Table 11>

&Quot; (2) &quot;

Average response rate (%) = [∑ (X score after 24 hours responded) + ∑ (X score after 24 hours responded)] × 100 / (total test x maximum score x total test)

Therefore, the average reaction rate of Preparation Example 1 was calculated as follows.

((15 people x 0 points + 0 people x 1 points + 0 people x 2 points + 0 people x 3 points + 0 people x 4 points) + (15 people x 0 points + 0 people x 1 point + 0 people x 2 Points + 0 points x 3 points + 0 points x 4 points)] x 100/2 times x 4 points x 20 points) = 0%

As a result, the results of the human patch test of the whitening cosmetics are shown in Table 12 below.

<Table 12>

* Criteria for the average response rate (%) of the human patch test

Less than 1: non-irritating less than 1: 3

3 ~ 5 or less: Medium stimulation 5 or more: Light stimulation

As can be seen from the results of Table 12, since the cosmetic composition for skin whitening according to the present invention belongs to the non-irritating range, it can be said to be a safe substance to the human body.

clinical Test Example  2. Whitening effect at human skin level

For 20 healthy men and women, opaque tape with 6 holes of 1.5 cm diameter was attached to the upper forearm of the subject, followed by 1.5 ~ 2 times the ultraviolet (UV) of each subject's minimum erythema (MED; Minimal Erythema Dose). -B) was investigated to induce pigmentation of the skin, and two months after the test substances were applied, the contrast of the skin was measured using a colorimeter. Each test substance was applied to the skin whitening cosmetic composition and the general skin whitening cosmetic composition containing a tangerine extract prepared according to the following Table 13 twice daily in the morning and evening, the determination of the effect of the skin contrast It was determined by obtaining an L * value representing. In general, the L * value of Korean skin that does not induce artificial pigmentation is in the range of 50 to 70. If the test substance is applied, the L * value gradually increases. The difference in skin color (ΔL *) between the start time and the completion time of application was calculated according to Equation 3 below, and the whitening effect on the skin of the human body was compared. The measurement results are shown in Table 14.

&Quot; (3) &quot;

ΔL * = L * value on the last day of test substance application-L * value before test substance application

<Table 13>

TABLE 14

Experimental results, as shown in Table 13, the skin whitening cosmetic composition (Preparation Examples 6 to 7) containing the mandibular extract according to the present invention as an active ingredient is superior to the general skin whitening cosmetic composition (Comparative Example 3) It was confirmed that a whitening effect appeared.

Claims (9)

100 parts by weight of purified water, 10 to 15 parts by weight of glycerin, 6 to 8 parts by weight of butylene glycol, 8 to 10 parts by weight of hyaluronic acid extract, 12 to 15 parts by weight of beta glucan, 0.15 to 0.2 parts by weight of chamoner, 0.01 to 20 parts of mandarin extract Parts by weight, 14 to 16 parts by weight of caprylic / capric trigly cerad, 8 to 10 parts by weight of squalane, 2.5 to 3 parts by weight of cetearyl glucoside, 0.5 to 1 parts by weight of sorbitan stearate and 0.15 to 0.2 of triethanolamine Made of parts by weight,
The mandarin extract is a skin whitening cosmetic containing a mandarin extract as an active ingredient, characterized in that it comprises one or more extracts selected from the group consisting of crude extract of mandarin, nonpolar solvent fraction of mandarin crude extract or polar solvent fraction of mandarin crude extract Composition.
delete delete delete delete The method according to claim 1,
The crude extract of the mandarin is obtained by extracting the mandarin with one or more solvents from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, ethyl acetate, butylene glycol, glycerin, ether and chloroform Cosmetic composition for skin whitening containing as an active ingredient.
The method according to claim 1,
The nonpolar solvent fraction of the crude mandarin orange extract is obtained by fractionating the crude extract of the mandarin orange with one nonpolar solvent selected from the group consisting of hexane, chloroform, methylene chloride, ethyl acetate, glycerin and butylene glycol. Cosmetic composition for skin whitening containing as an active ingredient.
The method according to claim 1,
The polar solvent fraction of the crude mandarin extract is obtained by fractionating the crude extract of the mandarin with one polar solvent selected from the group consisting of water and n-butanol. .
The method according to claim 1,
It includes a cosmetic composition for skin whitening containing the mandarin extract as an active ingredient,
Skin containing a tangerine extract as an active ingredient, comprising one formulation selected from the group consisting of lotion, skin softener, skin toner, astringent, cream, foundation, essence, pack, soap, cleansing foam, and body cleanser Whitening cosmetic composition.