KR100713030B1 - A purifying method of Canavalia gladiata DC having antimicrobial activity - Google Patents

Abstract

The present invention relates to a method of extracting the extract of Canavalia gladiata (Jacq.) DC having an antimicrobial activity against microorganisms causing skin diseases, wherein the extract of the soybeans purified by the method of the present invention is a tricophyton menta which causes athlete's foot. thereof has an excellent antibacterial activity against pie test (Trichophyton mentagrophytes), propynyl sludge tumefaciens arc Ness (Propionibacterium acnes) that cause acne. Therefore, the cosmetic composition prepared by containing the soybean extract purified by the method of the present invention has excellent efficacy against athlete's foot, acne, atopic or eczema skin.

Pea, Canavalia gladiata (Jacq.) DC, antimicrobial activity, athlete's foot, acne, cosmetic composition

Description

A purifying method of Canavalia gladiata DC having antimicrobial activity

1 is a diagram showing the antimicrobial activity by inhibiting the growth of Trichophyton mentagrophytes of soybean water extract, soybean propanol precipitate (control was not added to any sample),

Figure 2 is a diagram showing the acne improvement effect using a soap containing soybean extract.

The present invention relates to a method for purifying the extract of Canavalia gladiata (Jacq.) DC having antimicrobial activity against microorganisms causing skin diseases.

Athlete's foot is a genius ringworm that occurs on the palms, soles of the feet, between the fingers and toes, and is also called the hanposang ringworm (汗 泡 狀 白 癬). A bacterium called dermatophytes is an infection caused by the invasion of keratinized tissue layers. It often occurs in male adults and is less common in children. In particular, it occurs easily in a person who sweats a lot, and the affected part is the sole of the foot, the instep, the heel, the toe between the toe, and the like.

Athlete's foot can be classified into three types: clinically bullous, erosional, and keratinous. Blisters are small blisters or pustules on the toes, soles, and heel. At first, small blisters that are narrow are formed, and what is hidden in the skin gradually swells up and leaves a white rim. The lanceolate often occurs between the toes, especially between the fourth and the little toe. The skin becomes white as if it is stuck in the water, and it may cause tearing or reddening, cracking, and spread to the next toe. This type is sometimes difficult to distinguish from candidiasis. Lastly, keratin type occurs mainly on the soles and palms of the skin, and due to keratin proliferation, the skin becomes thick, thin, cracked, and the phenomenon of drought. These symptoms usually involve severe itching.

If you have athlete's foot, care of hands and feet is also important, but once athlete's foot is not easily cured, a fundamental solution is needed by using drugs or skin cosmetics that have antibacterial activity against athlete's foot.

Acne is a generic name for vulgar acne, which refers to inflammation of many hair follicles on the face, especially the cheeks and forehead of puberty. It may occur on the chest and back, and it may occur at age 40. Initially, the hair follicles are full of cortex and keratin, resulting in yellowish white masses, sometimes with blackened peaks. When you squeeze it will look like cheese. It is then inflamed and reddish. When a bacterium is infected, it is concentrated and turns yellow. The better spot is a crater-shaped trail. Usually four kinds are mixed. The cause is a lot of congenital traits and sex hormones, especially due to the effects of bacteria on the sebum secretion caused by the action of male hormones. Women 22 years and older often worsen just before menstruation.

Acne is also difficult to cure once it starts, so acne caused by bacterial infections are often treated with antibacterial and cosmetic beauty agents that have excellent antibacterial effects. There are not many products.

Atopy is a family or genetic allergic predisposition, named in 1925 by A. Coca of the United States for innate hypersensitivity to certain kinds of human-specific substances. The tendency to appear is high fever, asthma, atopic dermatitis, etc. in the family, high rate of intradermal reaction against various allergens (eg egg white, dandruff, pollen, dust, etc.), and antibodies in serum Abnormal response to the presence of (atopic allergen), blood eosinophilia, and various stresses (temperature, humidity, trauma, mental strain, infection, etc.).

Eczema is a type of skin disease, the cause of which is complicated. Eczema is a skin allergic disease caused by eczema stimulation (mechanical, physical, chemical stimulation from outside, drugs acting on the skin from the body, food, etc.). The name eczema has been used since the beginning of the 19th century, but at the time it was used as a name indicating only one type of skin lesion. Later, in the 1920s, animal experiments revealed that Hunt's phenomena varied, and the concept of allergy was introduced. As a result, inflammation caused by eczema and a distinct external person applied to the skin is caused by a person having a factor that is easy to break down. However, in recent years, allergies, etc. are difficult to distinguish between eczema and dermatitis are used in the same meaning. Symptoms of eczema are different and are largely divided into acute and chronic. In addition, due to the anatomical differences in age and body parts, and the difference in response and stimulation received from the outside, it causes unusual lesions.

Small beans ( Canavalia) gladiata (Jacq.) DC is a legume annual vine that blooms in June-July and bears fruit pods in August-October and ripens in late autumn. Fruits are bow-shaped and the shape is small, so called bean or dodu (刀豆). The length of ripe dried bean is 2-3.5cm, width 1-2cm, thickness 0.5-1.2cm.

The soybeans contain urease, hemaglutinine, canavaline and canavalia gibberellin I and II. In folk medicine, daughters, sinusitis, rhinitis and pertussis It is known that it is effective for the back pain. The nematicidal effect and the inhibitory activity against the nematode of S. soybean extracts were reported, and triterpene saponins were isolated and purified from soybeans for their structure, antihepatotoxic activity and antioxidant inactivation.

Accordingly, the present inventors have identified an optimal extraction method having antimicrobial activity against Athlete's foot or acne bacteria of the soybean extract, and the cosmetic composition containing the soybean extract extracted by the method is athlete's foot, acne as well as atopic or eczema The present invention was completed by confirming that it has an excellent effect.

An object of the present invention to provide a method for purifying the extract of Canavalia gladiata (Jacq.) DC having antimicrobial activity against microorganisms causing skin diseases.

In order to achieve the above object, the present invention provides a method for extracting a soybean extract having antimicrobial activity against Tricophyton mentagrophytes or Propiobacterium acnes.

The soybean extract is characterized in that extracted by soluble in water for 6-30 hours at 40-95 ℃ extracted by precipitation in propanol or acetone, and also extracts by the method described above.

The present invention also provides a cosmetic composition for athlete's foot, acne, atopic or eczema skin containing 1 to 30% by weight of the soybean extract.

The cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, cleansing foam, It means one of the formulations selected from the group consisting of cleansing lotion, cleansing cream, body lotion, body cleanser, shampoo and rinse.

Hereinafter, the present invention will be described in detail.

The present invention provides a method for extracting soybean extract having antimicrobial activity against Trichophyton mentagrophytes or Propiobacterium acnes.

Tracophyton Mentagrophytes are athlete's foot fungi that can be used as test specimens by obtaining TracoPiton Mentagrophytes KCTC 6085 from the Biotechnology Research Institute Gene Bank, and propiobacterium acnes is an acne bacterium. You can get propiobacterium acnes KCTC 3314 from the bank and use it as a test specimen.

Small beans ( Canavalia) gladiata (Jacq.) DC) can be used domestically or foreignly, and domestic is preferable. Any of beans, pods, stems and leaves of soybeans can be used.

The soybean extract of the present invention is at least one solvent selected from the group consisting of water, ethanol, methanol, acetone and propanol of 1 to 20 times, preferably 5 to 10 times the dry weight of the soybean, 6-30 at 40-95 ° C. Extraction can be effected by precipitation over time.

More specifically, the extraction method of the bean extract is solubilized in water at 40-95 ° C., preferably 60-80 ° C. for 6-30 hours, preferably 20-30 hours, and then acetone, propanol, ethanol, methanol It means to extract by precipitation in an organic solvent, preferably acetone or propanol, and also provides a soybean extract characterized in that the extraction by the above method.

The present invention also provides a cosmetic composition for athlete's foot, acne, atopic or eczema skin containing 1 to 30% by weight of the soybean extract.

The composition containing the extract of the present invention can be used in a variety of cosmetics, face wash and shampoo having an effect on athlete's foot, acne, atopic or eczema skin. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetic liquids.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , pyridoxine, pyridoxine, vitamin B ₁₂ , pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H and the like, and salts thereof (thiamine hydrochloride, sodium ascorbate salt) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. It is included in water soluble vitamins. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D ₂ , vitamin D ₃ , and vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherol vitamin E, DL-pantothenyl alcohol, D- Pantothenyl alcohol, Pantothenyl ethyl ether, etc.) are contained in the oil-soluble vitamin used by this invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be incorporated into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, lauric acid hexyl, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, lysinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactic acid, cetyl lactate, octyl lactate, trit citrate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methyl steoxysiloxane copolymer and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POEPOP (PolyoxyethylenePolyoxypropylene) Copolymer, POEPOPalkyl Ether, Polyether Modified Silicone, Lauric Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, coloramine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono 2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urocanine Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxy benzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% compounding.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to the soybean extract as an active ingredient, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments and flavorings. It includes.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specific examples of the cosmetic of the present invention include a flexible lotion, nourishing lotion, nourishing cream, nourishing lotion, cleansing cream, cleansing foam, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, pack, aftershave cream, sunscreen Sunscreen, suntan oil, body shampoo, hair shampoo, hair rinse, hand cream, foundation and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the following Examples and Experimental Examples are only examples of the present invention and the present invention is not limited thereto.

Reference Example  1. Preparation of the Experiment

Trichophyton mentagrophytes KCTC 6085 (Athletes) and Propionibacterium acnes (Propionibacterium acnes KCTC 3314; Acne Bacteria) used to measure the antimicrobial activity of the present invention (KCTC) As a medium used for culturing the test bacteria, potato dextrose medium was used for athlete's foot fungus, and reinforced clostridial medium was used for acne bacteria.

Example  1. Preparation of Pea Bean Extract at Various Temperatures

The soybeans were purchased in Korea, and the beans, pods, stems, and leaves of the soybeans were naturally dried and used as samples for extraction.

After drying, it was made into small pieces, dried weight 10g into 100ml of water and extracted for 24 hours at a temperature of 20, 40, 60, 80, 95 ℃ and filtered under reduced pressure to obtain a soybean extract for each temperature.

Experimental Example  1. Effect of Extraction Temperature on Antimicrobial Activity in Extracting Soybeans

The effect of extraction temperature on the antimicrobial activity of soybean extract was investigated.

1-1. Tricophyton Mencagrophytes  Occation

Tricopytone Mentagrophytes prepared in Reference Example 1 was used as a test bacterium by a cup method. Tricophyton Mentagrophytes is sterilized with potato dextrose medium adjusted to pH 5.8 and then inoculated with the test suspension, placing a cup on the surface of the plate medium, 200 µl of the pea extract obtained in Example 1 above. The antimicrobial activity against tricophyton mentagrophytes was measured by measuring the diameter of the growth-lowering ring formed after incubation.

1-2. Propionibacterium  Acnes

Propionibacterium acnes prepared in Reference Example 1 was used as a test bacterium by a cup method. Propionibacterium Acnes was sterilized with an enhanced Clostrial medium adjusted to pH 6.8, inoculated with the suspension of the test specimen, and a cup was placed on the surface of the plate medium, and 200 µl of the bean extract obtained in Example 1 was added thereto. After culturing in anaerobic conditions, the antimicrobial activity of propionibacterium acnes was measured by measuring the diameter of the growth ring.

As a result of the above experiment, as shown in Table 1 below, when the extraction temperature of the soybean extract of Trichophyton mentagrophytes and propionibacterium acnes was 60 ° C., the size of the growth resistant ring was 33.4 (mm), 32.3, respectively. It was confirmed that the antimicrobial activity of the soybean extract extracted at 60 ° C. was greater than that at other extraction temperatures (mm).

Effect of Extraction Temperature on Antimicrobial Activity in the Extract of Sorbus Beans Temperature (℃) Tricophyton Mentagrophytes (mm) Propionibacterium Acnes (mm) 20 25.2 24.3 40 30.6 27.8 60 33.4 32.3 80 32.5 30.5 95 31.7 31.6

Experimental Example  2. Effect of Extraction Time on Antimicrobial Activity in the Extract of Sorbus Beans

In order to examine the effect of the extraction time on the antimicrobial activity in the extract of the pea beans, based on the results of Experiment 1, the temperature of 60 ℃ which showed the most excellent antimicrobial activity as the extraction temperature, extraction time is 0, 6, 12 , 18, 24, 30 hours to prepare a soybean extract in the same manner as the method performed in Example 1 above.

The soybean extract prepared above was subjected to the same method as in Experimental Example 1 to determine the antimicrobial activity against Tricophyton mentagrophytes and propionibacterium acnes.

As a result of the above experiment, as shown in Table 2, when the extraction time of the soybean extract was performed for 24 hours, the diameters of growth-lowering rings for Tricophyton mentagrophytes and propionibacterium acnes were 33.2 ( mm), 32.7 (mm), which was the most excellent antimicrobial activity of the soybean extract extracted for 24 hours.

Effect of Extraction Time on Antimicrobial Activity in the Extracts Hours (hr) Tricophyton Mentagrophytes (mm) Propionibacterium Acnes (mm) 0 - - 6 27.3 20.6 12 28.6 26.8 18 30.9 31.3 24 33.2 32.7 30 32.5 32.6

Experimental Example  3. Effect of Precipitation Solvent on the Antimicrobial Activity in the Extract of Sorbus Beans

In order to examine the effect of the precipitation solvent on the antimicrobial activity in the extracts of the pea beans, based on the results of Experimental Example 1 and 2 in 50 ml of crude bean extract extracted with water as a solvent for 24 hours at a temperature of 60 ℃ Precipitated using two-fold acetone, propanol, methanol and ethanol organic solvent, and then the precipitate was dissolved in water to measure the antimicrobial activity. Antimicrobial activity measurement method was carried out in the same manner as the method performed in Experimental Example 1.

As a result of the above experiments, as shown in Table 3 below, tricophyton mentagrophytes and propionibacterium acnes exhibited superior antimicrobial activity when the soybean extract was precipitated using acetone and propanol than other solvents. It was. In the case of tricophyton mentagrophytes, the acetone-deposited soybean extract showed 50.8 (mm) and the propanol-seed soybean extract showed a growth-lowering ring diameter of 52.3 (mm), showing excellent antibacterial activity. It was confirmed that the precipitate shows the most excellent antimicrobial activity. In the case of propionibacterium acnes, the acetone precipitated bean extract was 47.9 (mm) and the propanol precipitated bean extract showed a growth inhibitory ring diameter of 48.8 (mm), showing excellent antibacterial activity. Precipitation showed the best antimicrobial activity.

Effect of Extraction Solvent on Antimicrobial Activity in the Extracts menstruum Tricophyton Mentagrophytes (mm) Propionibacterium Acnes (mm) Acetone 50.8 47.9 Propanol 52.3 48.8 ethanol 37.2 40.2 Methanol 38.7 37.6

Experimental Example  4. Antimicrobial Activity of Soap Prepared with Soybean Extract

An extraction temperature of 60 ° C. and an extraction time of 24 hours were obtained in the same manner as in Example 1, to obtain a crude soybean extract, which was precipitated with a propanol solvent. Soap was prepared. The raw materials used for soap production were used by dissolving the raw material of commercially available transparent soap, taking 20 g of the total weight of the soap, and dissolving the soybean propanol precipitate in water at 60-80 ° C. to 0% by weight, 0.5% by weight, and 1% by weight. Soap was prepared by adding%, 5%, 10%, and 30% by weight.

0.01 g of each soap prepared by the above method was taken and dissolved in 10 ml of water, and the antibacterial activity of the soap was measured in the same manner as in Experimental Example 1 above.

As a result of the experiment, the soap prepared by adding the soybean extract as shown in Table 4 exhibited antimicrobial activity by inhibiting the growth of tricophyton mentagrophytes, propionibacterium acnes. When the soybean extract was added at various concentrations, when the 1% by weight of the soybean extract was added to the total weight of the soap, the growth inhibition ring size was 38.4 (mm) in the case of tricophyton mentagrophytes and propionibacterium. Acnes showed the most excellent antimicrobial activity as 34.8 (mm), and when 5, 10, 30% by weight of soybean extract was added, it was confirmed that the antimicrobial activity was constant even though the concentration of the soybean extract increased.

Antimicrobial Activity of Soap Containing Pea Bean Extract % Added Tricophyton Mentagrophytes (mm) Propionibacterium Acnes (mm) 0 10.1 8.9 0.1 21.3 16.5 0.5 30.6 27.6 One 38.4 34.8 5 38.2 34.6 10 38.3 34.7 30 38.2 34.5

Experimental Example  5. Effect of Soap Prepared with Soybean Extract on Acne

After the soap containing 1% by weight of the soybean extract prepared by the method of Experimental Example 4 was used for a patient with acne for three days, the effect of the soap on the acne was directly photographed on the skin surface of the face.

As a result of performing the experiment, it was confirmed that acne is significantly improved compared to the control when the soybean soap of the present invention was used for 3 days as shown in FIG. 2.

Experimental Example  6. Effect on Soap Atopy and Eczema of Soap Prepared with Soybean Extract

According to the results of Experiment 4, the soap was prepared by adding 1% by weight of the soybean extract to the total weight of the soap to produce 20% of the atopy and eczema. The effects of soap on atopy and eczema skin were tested on the basis of very good, good, normal, bad, and very bad.

As a result of the above experiment, as shown in Table 5 below, when a person with atopy skin used a soap prepared by containing a soybean extract, in the case of irritation, 17 panelists felt good use without irritation, In the case of 15 people recognized the effect on the atopy skin, 14 people were good for the smell, 16 panels for the residual feeling felt no residual after using soap, 18 panels for the cleaning power This excellence was felt, and as shown in Table 6 above, when using the soap of the present invention, it was confirmed that most panels exhibited an effect on atopic skin within 3 days and at least 5 days.

In addition, as shown in Table 7 below, the soap prepared by containing the soybean extract was used by people with eczema, 17 people in case of irritation, 15 people in case of efficacy, 14 people in case of scent, and residual feeling In the case of 16 people, in the case of detergency, all 18 panels showed satisfaction with the soap of the present invention, and in the case of using the soap of the present invention, most panels could be effective within at least 3 days or at least 5 days. It could be confirmed. Therefore, the soap of the present invention was confirmed that the atopic dermatitis, eczema most people with excellent efficacy and cleansing power, it was confirmed that there is no specific side effects, stability and efficacy was verified.

Effect on Soap Atopy Containing Soybean Extract very good good is average bad Very bad stimulus 6 11 3 0 0 efficacy 7 8 4 One 0 Aroma 5 9 5 One 0 Residual 4 12 2 2 0 Cleaning power 6 12 2 0 0

How long it takes for atopy skin of soap containing soybean extract to be effective 3 days 5 days 7 days 10 days 15th Effect occurs 10 5 3 2 0

Effect on Eczema of Soap Containing Sorbus Beans very good good is average bad Very bad stimulus 6 13 One 0 0 efficacy 7 12 One 0 0 Aroma 4 11 4 One 0 Residual 6 11 2 One 0 Cleaning power 5 12 3 0 0

Time taken for effect to eczema of soap containing soybean extract 3 days 5 days 7 days 10 days 15th Effect occurs 12 5 3 0 0

As described above, the present invention has a soybean ( Canavalia) having antimicrobial activity against the microorganism causing the skin disease. A method for extracting gladiata (Jacq.) DC extract, which is soluble in water at 40-95 ° C. for 6-30 hours and then extracted by soaking in propanol or acetone. In addition, the bean extract extracted by the above method has excellent antimicrobial activity against Trichophyton mentagrophytes , which causes athlete's foot, and Propionibacterium acnes , which causes acne. Therefore, the cosmetic composition prepared by containing the soybean extract purified by the method of the present invention has excellent efficacy against athlete's foot, acne, atopic or eczema skin.

Claims (5)

Tracophytone mentagrophytes, which is characterized by drying beans, pods, stems and leaves of soybeans, extracting by solubility in water at 40-95 ° C. for 6-30 hours and then precipitating and extracting with propanol or acetone. Trichophyton mentagrophytes ) or Propionibacterium acnes ( Propionibacterium acnes ) antimicrobial activity of extracting soybean extract. delete A bean extract having antimicrobial activity against Trichophyton mentagrophytes or Propionibacterium acnes , characterized in that extracted according to the method of claim 1. Cosmetic composition for athlete's foot, acne, atopic or eczema skin, characterized in that containing 1 to 30% by weight of the soybean extract of claim 3. The composition of claim 4, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, an essence, a nutrient essence, a pack, Cosmetic composition for athlete's foot, acne, atopic or eczema skin, characterized in that the formulation of any one selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, shampoo and rinse.

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