JPH09143089A - Device for treating mrsa and therapeutic agent for mrsa - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a device for treating methicillin resistant Staphylococcus aureus(MRSA) having strong MRSA eliminating effects, capable of safely eliminating MRSA without adverse effects to human bodies and suitable for spraying into a mouth, nose, etc., by consisting of nebulizer and an extract of Asparasus linealis. SOLUTION: This device for treating MRSA comprises (A) an extract of Asparasus linealis [e.g. an extract obtained by heat-extracting its leaves, stems or fine powder (A1 ) with water or an organic solvent (e.g. methanol, ethanol or acetone), an extract prepared by extracting A1 with hot water, then extracting the residue with an alkali] and (B) a nebulizer such as an ultrasonic nebulizer. The component A can be singly administered by formulating in granules, powders, capsules, liniments, injections, etc., or used by mixing with other food and drink.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to MRSA (methicillin resistant Staphylococcus aureus).
ococcus aureus) therapeutic agent and MRSA therapeutic device, and a method of using Asparasus linearias extract for removing MRSA.

[0002]

BACKGROUND OF THE INVENTION MRSA (methicillin resistant Stapylococcus aureus) is
The number of third-generation cephem antibiotics has been rapidly increased since the 1980s, and the antibiotics cannot be removed by antibiotics, resulting in a serious social problem as a cause of nosocomial infections.

Conventionally, as a method for removing MRSA,
Infections are controlled by gowns, masks, washing hands, gargling with isodine gurgle, nasal disinfection with isodine, individualization of thermometers and blood pressure monitors, and individual room management.

[0004]

However, none of the methods can effectively remove MRSA, and antibiotics for removing MRSA also have side effects and are often dangerous to the human body. Furthermore, there is no known one that has a bactericidal effect or infection preventive effect on infected patients.

The present invention provides a method for using Asparasus linealis extract, which is harmless to the human body, can be eaten and eaten daily, has an MRSA infection-preventing effect, and has no side effects.
It is an object of the present invention to provide an MRSA therapeutic agent and a therapeutic device that effectively remove MRSA.

[0006]

Means for Solving the Problems Accordingly, the inventor of the present application has
As a result of intensive studies to solve the above-mentioned object, a therapeutic agent sprayed with a coniferous Aspalathus linealis extract belonging to the legume family, and a device consisting of an Aspalathus linealis extract and an ultrasonic nebulizer Is MRSA
The present invention has been completed based on the finding that MRSA can be safely removed with a strong removal effect and no side effects on the human body.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The active ingredient of the present invention comprises an extract of Aspalathus linealis, which belongs to the legume family.
Water or an organic solvent in an amount of 5 to 500 times these amounts is added to the fine powder of tea leaves, and heated at 40 to 100 ° C. for 5 to 3 hours for extraction.

As another extraction method, fine leaves, stems or tea leaf powder of Aspalathus linealis may be extracted with boiling water, and the extracted plant material may be dried and then extracted with alkali. . Alternatively, the active ingredient can be obtained by direct alkali extraction without the extraction process with hot water.

At this time, in the case of extraction with boiling water, water of 5 to 500 times that of Aspalathus linearis is added,
It is preferable to extract at -100 ° C for about 5-30 minutes. Also, when extracting with an alkaline solution, add 5 to 100 times the alkaline solution of dried leaves to the hot water extraction residue of Aspalathus linearias, and stir or shake at 20 to 80 degrees for about 1 to 4 hours for extraction. A liquid is obtained, and the alkali extract is neutralized and dried to obtain a dry powder.

Specific examples of the organic solvent include methanol,
Examples thereof include ethanol and acetone, and examples of the alkaline solution include sodium hydroxide (caustic soda), potassium hydroxide, ammonia, ammonium hydroxide, sodium carbonate, sodium hydrogen carbonate, and trishydroxyethylaminomethane (trade Tris). For extraction, conventional means such as warm extraction can be used. In addition, conventional means such as ordinary filtration, filtration using cotton, decantation and centrifugation can be used for separation of the extract. For drying, conventional means such as freeze-drying and spray-drying can be used.

The present invention is a therapeutic agent for MRSA which is used by spraying the obtained extract, but the spray needs to be used in the form of fine particles using an ultrasonic nebulizer or the like. A conventional nebulizer can be used, and the extract of Aspalathus linealis is set in the nebulizer and the mouth /
The finely atomized extract is inhaled through the nose.

The dose varies depending on the type and degree of the target disease, but when spraying using an ultrasonic nebulizer, 0.2-1% hot water extract (0.2 tea leaves / 100
(ml to 1 g tea leaf / 100 ml) is preferable. When using an alkali extract, a 0.01 to 0.5% dry powder solution is preferable. Further, if the spray solution is used by drinking in parallel with spraying, further removal effect can be expected.

For eating and drinking, the active ingredient of Aspalathus linealis is a physiologically acceptable carrier, excipient,
Orally or parenterally as binders and diluents, for example, granules, powders, hard capsules, soft capsules, liniments, syrups, suppositories, injections, or as a mixture, solutions or powders It can be used alone in any form such as granules, tablets, emulsions and jellies, can be used as a concentrated solution, or can be used by mixing with other foods and drinks.

The apparatus of the present invention is a combination of an extract of Aspalathus linearis and a nebulizer.
MRSA can be effectively removed by setting an extract of Aspalathus linealis as a substance to be sprayed in the nebulizer and inhaling fine particles of the extract of Aspalathus linealis into the mouth.

The spraying place should be in the form of fine particles in the mouth, nasal cavity,
It is preferable to spray into the throat, and the spraying time is 1
The amount of spraying is preferably 0 to 60 minutes and 5 to 50 ml. Also,
If the Aspalathus linealis extract is combined with a fine particle sprayer and sprayed on the whole body, not only the circulatory system but also the entire body can be sterilized, and the MRSA removal effect can be increased.

[0016] Aspalathus linealis showed no death in an acute toxicity test in rats, and no abnormality was observed in biochemical blood tests and histopathological tests.

[0017]

[Effects] According to the present invention, MRSA can be effectively removed without side effects, the infection can be prevented, and the growth of MRSA can be suppressed. Further, the effect can be increased by performing daily eating and drinking of the extract and spraying it on the body surface.

[0018]

【Example】

Production Example 1 Collected leaves of Aspalathus linealis (with stem)
After cutting to a length of 5 mm, the leaves are subjected to rolling, enzymes, fermentation, and sun drying to obtain dried leaves. 1.5 liters of water was added to 3.5 g of dried leaves and boiled for 10 minutes to obtain an extract.

Production Example 2 3.5 g of dried tea leaves of Aspalathus linealis collected
50 ml of 1% caustic soda solution at 45 ° C
Then, extraction was performed for 3 hours to obtain an extract. The obtained extract was dried with a freeze dryer to obtain a dry powder product.

Production Example 3 3.5 g of dried tea leaves of Aspalathus linealis collected
Was extracted with 500 ml of hot water at 85 ° C. for 3 hours, and then the leaves (with stem) were dried indoors. Dry leaf after extraction with hot water is added 4 times with 50 ml of 1% sodium hydroxide solution.
The mixture was vigorously shaken at 5 ° C. for 3 hours, cotton was laid on a funnel and filtered to obtain an extract. The extract was freeze-dried to obtain a dry powder of an alkaline extract of Aspalathus linealis.

Example 1 The extract of Aspalathus linealis obtained in Production Example 1 was sterilized and then sprayed onto a medium at various dilution concentrations to examine the growth condition of bacteria (minimum concentration of growth inhibition). Table 1 shows the minimum concentration of the extract that inhibits the growth of Escherichia coli.

[Table 1]

Example 2 20 ml of the undiluted solution of the extract obtained in Production Example 1 was suction sprayed onto a patient with an ultrasonic nebulizer. Spray 3 times a day,
It was carried out for 20 to 30 minutes each time. MRSA positive patient 15
Nine of them became negative within 2 weeks.

Example 3 A 0.05% solution of the dry powder obtained in Production Example 2 was suction-sprayed onto a patient by an ultrasonic nebulizer at a time of 20 ml. The spraying was performed 3 times a day. Of the 6 MRSA-positive patients, 4 became negative within 2 weeks.

Example 4 Using a therapeutic device consisting of a 0.05% solution of the dry powder obtained in Production Example 3 and an ultrasonic nebulizer, a patient was inhaled and sprayed according to Example 3. The spraying was performed 3 times a day. Eight of the 11 MRSA-positive patients became negative within 2 weeks.

Claims (8)

[Claims] 1. An MRSA treatment device comprising an Aspalathus linealis extract and a nebulizer. 2. The extract of Aspalathus linealis,
The MRSA treatment device according to claim 1, wherein the MRSA treatment device is obtained by alkali extraction. 3. The extract of Aspalathus linealis,
The MRSA treatment apparatus according to claim 1, wherein the residue is obtained by alkali extraction after hot water extraction. 4. A therapeutic agent for MRSA, which is used by spraying an extract of Aspalathus linealis. 5. The extract of Aspalathus linealis,
The therapeutic agent for MRSA according to claim 4, which is obtained by alkali extraction. 6. The extract of Aspalathus linealis,
The therapeutic agent for MRSA according to claim 4, wherein the residue is obtained by alkali extraction after hot water extraction. 7. The therapeutic agent for MRSA according to claim 4, which is sprayed into the mouth and nose with a nebulizer. 8. A method of using the extract of Aspalathus linealis, which comprises spraying the extract of Aspalathus linealis into the mouth and nose to remove MRSA.