KR20230022514A - Cosmetic composition comprising probiotics cultivatied using dogo hot spring water - Google Patents

Abstract

The present invention relates to a cosmetic composition which contains, as active ingredients: a culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo hot spring water; and a culture containing a strain of the genus Bifidobacterium cultured in a medium containing the Dogo hot spring water. The cosmetic composition of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has an ability to inhibit tyrosinase and melanin production, and thus has excellent skin whitening and wrinkle reduction effects.

Description

Cosmetic composition containing probiotics cultured using Dogo hot spring water {COSMETIC COMPOSITION COMPRISING PROBIOTICS CULTIVATIED USING DOGO HOT SPRING WATER}

The present invention relates to a cosmetic composition containing probiotics cultured using Dogo hot spring water, and more particularly, to a cosmetic composition containing probiotics cultured in a medium containing Dogo hot spring water and herbal medicines.

The skin is the largest organ in the body and always directly in contact with the external environment, playing the role of a protective film to protect the living body from various stimuli or dry environments. In addition, it protects the body from physical abrasions, prevents loss of moisture inside the body, protects from ultraviolet rays generated by the sun, and plays a very important role in regulating the body temperature.

Such skin causes problems such as skin contamination, dryness, trouble, skin immune system abnormality, etc. due to various physical factors, external environmental factors, infections, and the like. In particular, with the rapid industrial development of modern times, pollution problems such as air pollution and water pollution have become serious, and the strong cooling and heating of the living space due to the improvement of the residential environment causes the skin to become rough, dry, and aging. are greatly influenced by Human skin gradually becomes thinner due to intrinsic aging and photoaging, wrinkles increase, elasticity decreases, and spots and freckles increase due to exposure to ultraviolet rays. In particular, endogenous aging occurs when the secretion of hormones decreases or the function and activity of immune cells decrease, resulting in a decrease in the biosynthesis of biocomponent proteins, which progresses rapidly with an increase in active oxygen.

It is known that humans constantly generate reactive oxygen species in the metabolic process, which act as direct or indirect causes of aging. In order to prevent skin aging, various antioxidants are used in cosmetics, and typical synthetic antioxidants include butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effects and low prices, but their use is limited because they are known to cause liver enlargement, fatty mutations, and cancer as well as self-toxicity when in oral or skin contact.

Niacinamide and arbutin, which are used as raw materials for whitening functional cosmetics, and retinol, which are used as raw materials for wrinkle-improving functional cosmetics, have a problem in that the whitening effect is reduced due to the destruction of ingredients by oxidation and the restriction of the mixing amount due to skin irritation. Recently, as various cosmetic compositions using natural materials have appeared on the market, consumers have become interested in products using natural materials rather than products made of existing chemical materials, and accordingly, cosmetic compositions using various natural materials are being manufactured.

In addition, the anti-wrinkle effect of cosmetics is based on the effect of improving the dermis layer by various active ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and the growth of retinol, vitamin C, and EGF, which are representative raw materials of wrinkle-improving cosmetics, Factor components are well known to exhibit anti-wrinkle effects by increasing collagen generation from human dermal fibroblasts. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, physiologically active substances obtained from various known animals, plants, microorganisms, etc. are added to cosmetics and used to maintain the unique function of the skin and activate skin cells to Efforts have been made to effectively inhibit aging.

In the field of cosmetics, research and development on functional cosmetics having whitening and wrinkle-improving effects are being actively conducted, and in particular, studies using natural substances are continuing to avoid toxicity or irritation to the skin.

Korean Patent Publication No. 10-2021-0064818 Korean Patent Registration No. 10-1896942

An object of the present invention is to provide a cosmetic composition containing, as an active ingredient, a probiotics culture cultured using Dogo hot spring water and an herbal medicine extract, which has a skin whitening or wrinkle improvement effect.

According to one aspect of the invention,

Cultures containing strains of the genus Lactobacillus cultured in a medium containing Dogo hot spring water; and

A cosmetic composition containing as an active ingredient is provided; a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo hot spring water.

The medium containing the Dogo hot spring water may further include a herbal medicine extract containing at least one selected from the group consisting of mudanpi extract, gyejochuk extract, and yeongyo extract.

The herbal medicine extract may be extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The medium containing the Dogo hot spring water may contain 10 to 40% (v/v) of the Dogo hot spring water.

The medium containing the Dogo hot spring water contains 1 to 5% (v/v) of the herbal medicine extract, and the herbal medicine extract may have a solid content of 10 to 20% (w/v).

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) 1.0 to 2.0 mg/L.

The strains of the genus Lactobacillus include Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus harbinensis, Lactobacillus casei , Lactobacillus sake ( Among Lactobacillus sakei ), Lactobacillus brevis , Lactobacillus pentosus , Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus It can be any one selected.

The Bifidobacterium genus strains include Bifidobacterium longum, Bifidobacterium breve , Bifidobacterium lactis, and Bifidobacterium bifidum. ( Bifidobacterium bifidum ) It may be any one selected from.

The cosmetic composition may be used for skin whitening or wrinkle improvement.

The cosmetic composition containing probiotics cultured using Dogo hot spring water of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, so it has excellent skin whitening and wrinkle improvement effects.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, a cosmetic composition containing probiotics cultured using the Dogo hot spring water of the present invention will be described.

The cosmetic composition of the present invention is a culture containing a strain of the genus Lactobacillus cultured in a medium containing Dogo hot spring water; And a culture containing a strain of the genus Bifidobacterium cultured in a medium containing Dogo hot spring water; characterized in that it comprises as an active ingredient. The strain cultured in the culture is characterized in that it is used as an active ingredient of cosmetics without being removed.

Preferably, the medium containing the Dogo hot spring water may further include a herbal medicine extract containing at least one selected from mudanpi extract, gyaejochuk extract and yeongyo extract, more preferably danpi extract, gyaejochuk extract and It is possible to use three types of complex herbal medicines containing Yeongyo extract. If any one of the three types is excluded, the wrinkle improvement or whitening effect may be greatly reduced compared to the case of using the three types of compound herbal medicine.

The extract of medicinal herbs including Mokdanpi, Gyejochuk, and Yeongyo may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably a hot water extract.

Moutan bark is a medicinal material made from the root bark of the peony (Paeonia suffruticosa Andrews) of the peony family, and is used in China and Japan as well as in Korea. Mokdanpi is used for menstrual irregularities due to blood fever, menstrual cramps, bruises, hematemesis, nosebleeds, spots, bone aches due to fever, blood pressure increase, blood elimination, bruises, anti-inflammatory analgesia, abscess treatment, and early appendicitis. It is known to relieve chest discomfort. As for the pharmacological action, analgesic, sedative, antipyretic, anticonvulsant, anti-inflammatory, antithrombotic, anti-allergic, gastric juice secretion inhibition, uterine mucosal congestion, and antibacterial action have been reported. Appearance is a piece of tubular bark, the outer surface is dark brown or purplish brown, and there are horizontally long and small oval side root marks and vertical wrinkles. The inner surface is light grayish brown or dark purple, flat, and the bent surface is rough. White crystals are sometimes attached to the inside and bent sides.

The gradation axis is Manshurian fullmoon maple of the Maple family, and its scientific name is Acer pseudo-sieboldianum (Paxton) Komarov. Deciduous broad-leaved small tree, height of about 8m, leaves are 7-10cm long, divided into 9-10 branches, hairs grow densely on the backside of young leaves and branches, flowers are hermaphrodite, magenta in May, calyx is There are 5-6 hairs, and the petals are composed of 4 sheets. The bark part of the herbal medicinal material is used, and the bark part is also used for the extract of the goldenrod of the present invention.

Yeongyo refers to the dried fruit of Forsythia viridissima Lindley or Forsythia suspensa Vahl of the ash family. Yeongyo got its name because the fruit forms a room similar to a lotus flower and protrudes long among various grasses. It has a peculiar smell, the taste is bitter, and the energy is slightly cold. It is known that it has the effect of lowering heat and detoxifying, eliminating wind fever, removing boils or wounds, or releasing clumps or lumps. As for the pharmacological action, antibacterial action, anti-inflammatory action, blood pressure lowering, hemostatic action, liver treatment action, antipyretic action, antiemetic action, and diuretic action have been reported. Major chemical components include forsythol, sterol compounds, saponin, and oleanolic acid.

The medium containing the Dogo hot spring water preferably contains 10 to 40% (v/v) of the Dogo hot spring water, more preferably 25 to 35% (v/v). If it is less than 10% (v/v), wrinkle improvement or whitening effect may be reduced, and if it exceeds 40% (v/v), it may be difficult to culture probiotics.

In addition, the medium containing the Dogo hot spring water contains 1 to 5% (v / v) of the herbal medicine extract, the herbal medicine extract preferably has a solid content of 10 to 20% (w / v), more preferably Contains 2 to 4% (v / v) of the herbal medicine extract, and the herbal medicine extract may have a solid content of 12 to 17% (w / v). If the herbal medicine extract is included in less than 1% (v / v), wrinkle improvement or whitening effect may be reduced, and if it exceeds 5% (v / v), skin toxicity may occur or probiotics may not be cultured properly. may not be

The strains of the genus Lactobacillus include Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus harbinensis , Lactobacillus casei , Lactobacillus sake ( Among Lactobacillus sakei ), Lactobacillus brevis , Lactobacillus pentosus , Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus It is preferably any selected one, more preferably Lactobacillus plantarum ( Lactobacillus plantarum ).

The Lactobacillus plantarum is a bacterium that is safe enough to produce useful metabolites for the human body and to be used as a food raw material. The Lactobacillus plantarum culture extract of the present invention is obtained by culturing the Lactobacillus plantarum and disrupting the cells with a homogenizer, and contains metabolic components, cytoplasm, cell wall components, polysaccharides, etc. of Lactobacillus plantarum It is a fermentation lysate in

In addition, the strains of the Bifidobacterium genus include Bifidobacterium longum, Bifidobacterium breve , Bifidobacterium lactis , and Bifidobacterium It is preferably any one selected from Bifidobacterium bifidum , and more preferably Bifidobacterium longum .

Bifidobacterium longum ( Bifidobacterium longum ) is a gram-positive lactic acid bacterium that exists in the human gastrointestinal tract and has been isolated from the human body and has proven its stability, and is also known as a beneficial bacterium that improves the intestinal environment.

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) characterized in that it comprises 1.0 to 2.0 mg / L.

The cosmetic composition of the present invention is characterized in that it is for skin whitening or wrinkle improvement.

The term "extract" used herein includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of the extract. For example, the extract can be prepared in a powder state by additional processes such as vacuum distillation and freeze drying or spray drying.

In addition, the herbal medicine extract including Mokdanpi, Gyejochuk, and Yeongyo of the present invention has the meaning of including formulated processed products, such as herbal medicine powder, in a broad sense. Although the experiment was conducted with herbal medicines in the present invention, it would be expected by those skilled in the art that the desired effect could be achieved even in the same form as the herbal medicine processed product.

On the other hand, in the present specification, the term "comprising as an active ingredient" means including a sufficient amount to achieve the efficacy or activity of the ingredient containing the probiotics culture. For example, it is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml, including the probiotics culture. Since the probiotics culture is a natural product and does not have side effects on the human body even when applied to the skin in excess, the quantitative upper limit of the probiotics culture included in the composition of the present invention can be selected and implemented by those skilled in the art within an appropriate range.

The term "skin whitening" used in the present invention is to whiten the skin, and the skin color of a person is determined by the concentration and distribution of melanin inside the skin. In addition to genetic factors, solar ultraviolet radiation, fatigue, stress, etc. are also affected by environmental or physiological conditions. Melanin is synthesized in melanocytes in the epidermal layer of the skin. An enzyme called tyrosinase acts on tyrosine, a kind of amino acid, in melanosomes, organelles in melanocytes, to produce DOPA and dopaquinone. ) and then produced through a non-enzymatic oxidation reaction. When such melanin synthesis occurs excessively in the skin, the skin tone is darkened, and spots, freckles, and the like are generated. Therefore, if the synthesis of melanin pigment is inhibited by inhibiting the activity of tyrosinase in the skin, skin whitening can be realized by brightening the skin tone, as well as skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes. It can improve depression.

In addition, the term "wrinkle" in the present invention refers to fine lines caused by skin deterioration, which may be caused by genes, reduction of collagen present in the skin dermis, external environment, and the like. In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles already formed.

The main function of skin fibroblasts is to regenerate damaged skin tissue through extracellular matrix synthesis and proliferation. Extrinsic aging factors such as photoaging or endogenous aging factors such as accumulation of free oxygen damage and cell aging due to telomere disruption They lower the physiological activity of dermal fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of skin extracellular matrix and plays a very important part in the physiological activity of fibroblasts through interaction and signal exchange with adhesion molecules and cytoskeleton of cells When the synthesis of collagen is reduced, the amount of collagen in the skin tissue is reduced, and as a result, the surface tension is reduced, resulting in a decrease in skin elasticity and formation of skin wrinkles, as well as a decrease in physiologically active functions including cell proliferation and regeneration. cause a vicious cycle of

Therefore, collagen of dermal fibroblasts can be directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration upon skin damage. That is, when the synthesis of collagen or procollagen in skin fibroblasts is promoted or the synthesis of matrix metallo-proteinase (hereinafter referred to as 'MMP') that degrades collagen is inhibited, skin elasticity is improved, skin regeneration, skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And effects such as skin aging prevention can be obtained.

The cosmetic composition of the present invention is characterized by increasing the expression of type I procollagen protein and suppressing the expression of MMP-1 protein. Specifically, the cosmetic composition of the present invention suppresses the expression of matrix metallo-proteinase, which promotes collagen degradation, while increasing the production of type I procollagen, thereby increasing skin elasticity and regenerating skin. , skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And it may have effects such as skin aging prevention. That is, it may have an effect of preventing or improving skin wrinkles.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts.

Any water-soluble vitamin may be used as long as it can be formulated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H and the like, and their salts (thiamin hydrochloride, ascorbic acid sodium salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) can also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamin can be obtained by conventional methods such as microbial transformation, microbial culture purification, enzymatic or chemical synthesis.

The fat-soluble vitamin may be any substance as long as it can be formulated into cosmetics, but preferably includes vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc., and their derivatives (ascorbic acid palmitate, ascorbic acid stearate). Bean, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymer peptide may be anything as long as it can be incorporated into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. The polymeric peptide can be purified and obtained by a conventional method such as a microbial culture solution purification method, an enzyme method, or a chemical synthesis method, or can be used after being purified from a natural product such as pig or cow dermis or silkworm silk fiber.

The polymeric polysaccharide may be anything as long as it can be formulated into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingolipid may be any material as long as it can be formulated into cosmetics, but preferably includes ceramide, phytosphingosine, sphingosaccharide lipid, and the like. The sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast or plants.

The seaweed extract may be anything as long as it can be formulated into cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc., and also calrageenan, arginic acid, and arginic acid purified from these seaweed extracts Sodium, potassium alginate, etc. are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, in addition to the above essential components, other ingredients commonly used in cosmetics may be mixed as necessary.

Other ingredients that may be added include fats and oils, humectants, emollients, ultraviolet absorbers, pH adjusters, fragrances, blood circulation promoters, cooling agents, antiperspirants, and purified water.

Examples of the oil and fat component include ester oil, hydrocarbon oil, silicone oil, fluorine oil, animal fat and vegetable oil.

Examples of the ester-based oils include tri-2-ethylhexanoate glyceryl, 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearate isocetyl, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipate, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, tetraethyl 2-ethylhexanoate pentaelli Slitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, lauric acid sostearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Tostearyl, 2-ethylstearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl dioctanoate Glycol, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl neodecanoate Decyl, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, triisocetyl citrate, triisoalkyl citrate, Triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearate 2- Ethylhexyl, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, isostear Cholesteryl acid, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phyteryl isostearate, physteryl oleate, isocetyl 12-stealoylhydroxystearate, 12 - Ester systems, such as stearyl stealoyl hydroxy stearate and isostearyl 12-stealoyl hydroxy stearate, etc. are mentioned.

Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and vaseline.

Examples of the silicone-based oil include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based fats and oils include perfluoropolyether and the like.

The animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker daemia nut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange raffia oil, jojoba oil, candelilla wax, carnaba wax, liquid lanol , animal or vegetable fats and oils such as hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

The water-soluble low-molecular moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactate, etc. are mentioned.

Examples of the fat-soluble low-molecular moisturizers include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, etc. can be heard

Examples of the fat-soluble polymer include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymeric silicone, and the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

The ultraviolet absorber includes para-aminobenzoic acid, para-aminobenzoic acid ethyl, para-aminobenzoic acid amyl, para-aminobenzoic acid octyl, salicylic ethylene glycol, salicylic acid phenyl, salicylic acid octyl, salicylic acid benzyl, salicylic acid butylphenyl, salicylic acid homomentyl, Benzyl cinnamate, paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl-diisopropylcinnamic acid ester mixture , urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Cosmetics prepared by containing the cosmetic composition of the present invention may take the form of solutions, emulsions, viscous mixtures, and the like.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above ingredients as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural flavors, and A carrier may be further included.

The cosmetic composition of the present invention can be used in various ways such as cosmetics or facial cleansers having skin whitening or wrinkle improvement effects.

Products to which the cosmetic composition of the present invention can be added include, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, and ultraviolet rays. There are cosmetics such as blocking cream, moisture cream, hand cream, foundation, essence, nutrient essence, and mask pack, as well as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or a propellant such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention, but the following examples are merely illustrative of the present invention, and various changes and modifications are possible within the scope and spirit of the present invention. It is obvious to those skilled in the art, It goes without saying that these variations and modifications fall within the scope of the appended claims.

[Example]

Preparation Example A: Mudanpi extract

Moutan bark was cut into small pieces, followed by hot water extraction at 100 ° C for 4 hours with water at a ratio of 1:10 (w/v), filtering through non-woven fabric to remove impurities, and concentration under reduced pressure to obtain a solid content of 15% or more to prepare the extract of Moutan bark. did

Preparation Example B: Gyejo axis extract

An extract was prepared by extracting under the same conditions as in Preparation Example A, except that the bark of the gray axis was used instead of the mudan bark.

Preparation Example C: Yeongyo Extract

A Forsythiae Fructus extract was prepared by extracting under the same conditions as in Preparation Example A, except that Forsythiae Fructus was used instead of Moutanpi.

Preparation Example 1: Lactobacillus plantarum culture using Dogo hot spring water

Lactobacillus plantarum strain was inoculated with 1 ml of Lactobacillus plantarum 10 ⁸ CFU/ml) in a mixed medium mixed with 70 ml of MRS Broth liquid medium and 30 ml of Dogo hot spring water, and incubated at 37 ° C for 30 hours. After fermentation, the culture was disrupted with a homogenizer to obtain a Lactobacillus plantarum culture.

Preparation Example 2: Bifidobacterium longum culture using Dogo hot spring water

A Bifidobacterium longum culture was obtained by culturing under the same conditions as in Preparation Example 1, except that the Bifidobacterium longum strain was used instead of Lactobacillus plantarum.

Preparation Example 3: Lactobacillus plantarum culture using Dogo hot spring water + herbal medicine composite extract

Except for using a mixed medium in which 70 ml of MRS Broth liquid medium, 27 ml of Dogo hot spring water, and 3 ml of herbal medicine complex extract were used instead of a mixed medium mixed with 70 ml of MRS Broth liquid medium and 30 ml of Dogo hot spring water, Preparation Example 1 and Lactobacillus plantarum cultures were obtained under the same conditions. Here, the herbal medicine composite extract is a mixture of the Moutan bark extract of Preparation Example A, the Gyejochuk extract of Preparation Example B, and the Yeongyo extract of Preparation Example C at a volume ratio of 1: 1: 1.

Preparation Example 4: Bifidobacterium longum culture using Dogo hot spring water + herbal medicine complex extract

A Bifidobacterium longum culture was obtained by culturing under the same conditions as in Preparation Example 3, except that the Bifidobacterium longum strain was used instead of Lactobacillus plantarum.

Comparative Preparation Example 1: Lactobacillus plantarum cell removal culture using Dogo hot spring water

After fermentation, instead of treating the culture with a homogenizer to disrupt the cells, the culture was prepared in the same manner as in Preparation Example 1, except that the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 2: Bifidobacterium longum cell removal culture using Dogo hot spring water

After fermentation, instead of treating the culture with a homogenizer to disrupt the cells, the culture was prepared in the same manner as in Preparation Example 2, except that the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 3: Lactobacillus plantarum cell removal culture using Dogo hot spring water + herbal medicine composite extract

After fermentation, instead of treating the culture with a homogenizer to disrupt the cells, the culture was prepared in the same manner as in Preparation Example 3, except that the culture was centrifuged to obtain a filtrate from which the cells were removed.

Comparative Preparation Example 4: Removal of Bifidobacterium Cells Longum Culture Using Dogo Hot Spring Water + Herbal Medicine Complex Extract

A Bifidobacterium longum culture was obtained by culturing under the same conditions as in Preparation Example 4, except that the Bifidobacterium longum strain was used instead of Lactobacillus plantarum .

Example 1: Mixed culture using Dogo hot spring water

A mixed culture was prepared by mixing the Lactobacillus plantarum culture of Preparation Example 1 and the Bifidobacterium longum culture of Preparation Example 2 at a volume ratio of 1:1.

Example 2: Mixed culture using Dogo hot spring water + complex herbal medicine

A mixed culture was prepared by mixing the Lactobacillus plantarum culture of Preparation Example 3 and the Bifidobacterium longum culture of Preparation Example 4 at a volume ratio of 1:1.

Comparative Example 1: Mixed culture using Asan hot spring water

A mixed culture was prepared under the same conditions as in Example 1, except that Asan hot spring water was used instead of Dogo hot spring water.

Comparative Example 2: Mixed culture using Asan hot spring water + composite herbal medicine

A mixed culture was prepared under the same conditions as in Example 1, except that Asan hot spring water was used instead of Dogo hot spring water.

Comparative Example 3: Mixed culture using Onyang hot spring water

A mixed culture was prepared under the same conditions as in Example 1, except that Onyang hot spring water was used instead of Dogo hot spring water.

Comparative Example 4: Onyang hot spring water + mixed culture using complex herbal medicine

A mixed culture was prepared under the same conditions as in Example 1, except that Onyang hot spring water was used instead of Dogo hot spring water.

Comparative Example 5: Cell removal mixed culture using Dogo hot spring water

A mixed culture was prepared by mixing the Lactobacillus plantarum cell removal culture of Comparative Preparation Example 1 and the Bifidobacterium longum cell removal culture of Comparative Preparation Example 2 at a volume ratio of 1:1.

Comparative Example 6: Cell removal mixed culture using Dogo hot spring water + complex herbal medicine

A mixed culture was prepared by mixing the Lactobacillus plantarum cell removal culture of Comparative Preparation Example 3 and the Bifidobacterium longum cell removal culture of Comparative Preparation Example 4 at a volume ratio of 1:1.

Comparative Example 7: Mixed culture using Dogo hot spring water + Moutan skin

A mixed culture was prepared under the same conditions as in Example 2, except that the mudanpi extract of Preparation Example A was used alone instead of the composite herbal medicine extract.

Comparative Example 8: Mixed culture using Dogo hot spring water + gradation axis

A mixed culture was prepared under the same conditions as in Example 2, except that the Gyejo axis extract of Preparation Example B was used alone instead of the composite herbal extract.

Comparative Example 9: Mixed culture using Dogo hot spring water + Yeongyo

A mixed culture was prepared under the same conditions as in Example 2, except for using the Yeongyo extract of Preparation Example C alone instead of the composite herbal extract.

Comparative Example 10: Mixed culture using Dogo hot spring water + Moutan bark / Gyejo axis

A mixed culture was prepared under the same conditions as in Example 2, except that an extract obtained by mixing the extract of Moutan bark of Preparation Example A and the extract of Gyejo axis of Preparation Example B at a volume ratio of 1: 1 was used instead of the composite herbal extract.

Comparative Example 11: Mixed culture using Dogo hot spring water + Mudanpi / Yeongyo

A mixed culture was prepared under the same conditions as in Example 2, except that an extract obtained by mixing the extract of Mokdanpi of Preparation Example A and the extract of Yeongyo of Preparation Example C at a volume ratio of 1: 1 instead of the composite herbal extract was used.

Comparative Example 12: Mixed culture using Dogo hot spring water + gradation axis / year bridge

A mixed culture was prepared under the same conditions as in Example 2, except that an extract obtained by mixing the extract of Yeongyo of Preparation Example B and the extract of Preparation Example C of Yeongyo of Preparation Example C at a volume ratio of 1: 1 instead of the composite herbal extract was used.

Comparative Example 13: Single culture using Dogo hot spring water + complex herbal medicine

A single culture of Lactobacillus plantarum prepared according to Preparation Example 3 was used.

Comparative Example 14: Single culture using Dogo hot spring water + complex herbal medicine

A single culture of Bifidobacterium longum prepared according to Preparation Example 4 was used.

Culture preparation conditions of Preparation Examples 1 to 4, Comparative Preparation Examples 1 to 4, Examples 1 and 2, and Comparative Examples 1 to 12 are summarized in Tables 1 and 2 below.

division Hot spring water with badge fermentation strain Herbal medicine with medium Inclusion of culture cells Preparation Example 1 Dogo hot spring water Lactobacillus Plantarum doesn't exist include Preparation Example 2 Dogo hot spring water Bifidobacterium longum doesn't exist include Preparation Example 3 Dogo hot spring water Lactobacillus Plantarum 3 types complex
(Production Examples A, B, C) include Production Example 4 Dogo hot spring water Bifidobacterium longum 3 types complex
(Production Examples A, B, C) include Comparative Preparation Example 1 Dogo hot spring water Lactobacillus Plantarum doesn't exist not included Comparative Preparation Example 2 Dogo hot spring water Bifidobacterium longum doesn't exist not included Comparative Preparation Example 3 Dogo hot spring water Lactobacillus Plantarum 3 types complex
(Production Examples A, B, C) not included Comparative Preparation Example 4 Dogo hot spring water Bifidobacterium longum 3 types complex
(Production Examples A, B, C) not included

division Hot spring water with badge fermentation strain Herbal medicine with medium Inclusion of culture cells Example 1 Dogo hot spring water 2 types doesn't exist include Example 2 Dogo hot spring water 2 types 3 types complex
(Production Examples A, B, C) include Comparative Example 1 Asan hot spring water 2 types doesn't exist include Comparative Example 2 Asan hot spring water 2 types 3 types complex
(Production Examples A, B, C) include Comparative Example 3 Onyang hot spring water 2 types doesn't exist include Comparative Example 4 Onyang hot spring water 2 types 3 types complex
(Production Examples A, B, C) include Comparative Example 5 Dogo hot spring water 2 types doesn't exist not included Comparative Example 6 Dogo hot spring water 2 types 3 types complex
(Production Examples A, B, C) not included Comparative Example 7 Dogo hot spring water 2 types 1 type
(Production Example A: Moutan Skin) include Comparative Example 8 Dogo hot spring water 2 types 1 type
(Production example B: gradation axis) include Comparative Example 9 Dogo hot spring water 2 types 1 type
(Manufacture Example C: Yeongyo) include Comparative Example 10 Dogo hot spring water 2 types 2 types
(Manufacturing example A, B: Mokdan skin, gradation axis) include Comparative Example 11 Dogo hot spring water 2 types 2 types
(Manufacturing Examples A, C: Moutan skin, Yeongyo) include Comparative Example 12 Dogo hot spring water 2 types 2 types
(Manufacturing example B, C: gradation axis, year bridge) include Comparative Example 13 Dogo hot spring water single
(Lactobacillus plantarum) 3 types complex
(Production Examples A, B, C) include Comparative Example 14 Dogo hot spring water single
(Bifidobacterium longum) 3 types complex
(Production Examples A, B, C) include

For reference, the results of comparing the mineral components and contents of Dogo hot spring water used as a medium component in Examples, which are the three major hot spring waters distributed in the Asan region, and Asan hot spring water and Onyang hot spring water used in Comparative Examples are shown in Table 3 below. . The results in Table 3 are quoted from a known project report related to "development of skin-improving cosmetics using hot spring water in Chungcheongnam-do."

[Experimental example]

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). A one-way ANOVA with Duncan's post-hoc test was used to determine the difference (p <0.05) in the mean value between the experimental groups. Data from each experiment were expressed as mean ± standard deviation (SD).

Experimental Example 1: Cytotoxicity assay

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method. The cultured HaCaT cells were mixed well in DMEM medium, adjusted to 1x10 ⁵ cells/mL, and then 1 mL of each prepared cell was added to a 24-well culture plate and cultured for 3 days. Samples of Examples 1 and 2 and Comparative Examples 1 to 4 (0, 25, 50, 100, 300, 400, 500, 1000 [mu]g/ml), the cells were further cultured for 24 hours, and the viability of the cultured cells was measured using the MTT reduction method.

After adding about 1/10 of the volume of the growth medium to each well, the MTT solution (5 mg/ml) was further incubated at 37 °C for 4 hours. Thereafter, the medium was removed while being careful not to lose the formazan produced by reducing MTT, and after leaving the medium at room temperature for 30 minutes to completely remove the remaining medium, the sample was dissolved using DMSO and then 570 nm The absorbance was measured at. Cell viability was calculated according to Equation 1 below, and the results are shown in Table 4.

[Equation 1]

Cell viability (%) = [absorbance of sample treatment group/absorbance of control group] x 100

division Concentration (μg/ml) 10 100 untreated 100±1.1 Example 1 97.8±1.3 93.1±1.4 Example 2 95.7±1.9 90.3±0.5 Comparative Example 1 98.2±0.5 92.1±0.9 Comparative Example 2 96.1±2.4 91.3±1.0 Comparative Example 3 97.7±0.5 92.8±1.1 Comparative Example 4 96.3±1.0 90.5±0.8

According to this, it was confirmed that not only the cultures according to Examples 1 and 2 of the present invention, but also the cultures of Comparative Examples 1 to 4 can be safely used on human skin. Since the cultures of Comparative Examples 5 to 12 are included in the components of Examples 1 and 2 or other Comparative Examples, separate cytotoxicity was not performed.

Experimental Example 2: MMP-1 synthesis inhibitory effect analysis

CCD-986sk was cultured in a 35 mm dish at a concentration of 1.5X10 ⁵ cells/ml until reaching about 80% confluency. After removing the original medium, washing with PBS to remove the serum component in the medium, UVA (6.3J/cm2) was irradiated for 24 hours using a UV light source (Coralife, 35W, UV) equipped with a filter. . After UVA irradiation, the samples of Examples or Comparative Examples of the present invention were administered to each concentration of 10 and 100 μg/ml in a DMEM medium without FBS, and cultured for 24 hours. The cultured medium was dispensed into a 96 well plate and reacted overnight at 4 °C. MMP-1 expression level induced by UVA irradiation was measured using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate bufferedsaline + 0.05% Tween20), and then blocked with 3% PBS at 37 °C for 1 hour. Thereafter, a solution diluted with a blocking buffer of a primary antibody (monoclonal anti-MMP-1 antibody) at a volume ratio of 1:3000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, a solution in which a secondary antibody (alkaline phosphatase conjugated Goat anti-mouse IgG) was diluted with a blocking buffer at a volume ratio of 1:3,000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, alkaline phosphatase substrate solution (1 mg/ml, p-mitrophenylphosphate in diethanolamine buffer) was added, followed by reaction at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, absorbance was measured at 405 nm using a microplate reader, and the results are shown in Table 5 below.

division Concentration (μg/ml) 10 100 untreated 8760±15 Example 1 6820±42 5320±94 Example 2 4802±82 3306±38 Comparative Example 1 7140±90 6206±72 Comparative Example 2 5390±101 3828±45 Comparative Example 3 6900±42 6724±88 Comparative Example 4 5208±70 4109±54 Comparative Example 5 7840±110 6722±72 Comparative Example 6 6257±75 6563±49 Comparative Example 7 6724±40 5327±49 Comparative Example 8 6520±66 5890±121 Comparative Example 9 6054±79 5807±20 Comparative Example 10 5520±61 5090±102 Comparative Example 11 6254±33 5944±51 Comparative Example 12 6800±43 5583±99 Comparative Example 13 7504±47 7210±43 Comparative Example 14 7663±58 7123±28

According to this, the mixed culture using two fermented strains and the culture of Example 2 including three kinds of complex herbal medicines in the culture were measured to have a very high inhibitory effect on the synthesis of MMP-1 protein compared to other experimental groups, and 3 Even when one or two kinds of herbal medicines other than species complex herbal medicines were included in the medium, the synthesis inhibitory effect of MMP-1 protein did not show a significant difference compared to Example 1 in which herbal medicines were excluded. In addition, Comparative Example 2 and Comparative Example 4 including Asan or Onyang hot spring water including three kinds of herbal medicines also showed excellent inhibitory effect on the synthesis of MMP-1 protein, but showed a relatively low effect compared to Example 2.

In addition, in the case of the cultures of Comparative Examples 5 and 6 in which the cells were removed from the culture, the synthesis inhibitory effect of the MMP-1 protein was lower than that of Examples 1 and 2 containing the cells, and when only one fermentation strain was used, It seems to be effective in improving wrinkles as it has the lowest effect of inhibiting the synthesis of MMP-1 protein, a collagen degrading enzyme.

Experimental Example 3: Collagen synthesis promotion effect analysis

In order to confirm the skin wrinkle improvement effect of the cosmetic compositions of Examples and Comparative Examples, the amount of protein collagen produced to maintain skin elasticity was measured. Fibroblasts were dispensed at a density of 1X10 ⁴ cells/well in a 48 well plate, cultured for 24 hours in DMEM medium containing 10% FBS, and samples of Examples or Comparative Examples were added at 50 and 100 μg/ml, and no Incubated for an additional 24 hours in serum medium. After incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using the procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted into ng/ml. , The amount of collagen synthesized thereby was measured, and the results are shown in Table 6.

division Concentration (μg/ml) 50 100 untreated 40.1±0.8 Example 1 60.8±1.7 75.9±1.4 Example 2 82.2±1.2 92.4±0.8 Comparative Example 1 49.8±1.2 52.9±1.5 Comparative Example 2 77.7±0.8 88.2±1.3 Comparative Example 3 47.5±1.1 55.7±0.3 Comparative Example 4 80.6±0.5 90.2±0.7 Comparative Example 5 49.5±1.3 54.7±0.4 Comparative Example 6 50.6±0.8 52.8±0.8 Comparative Example 7 61.8±1.0 73.5±1.6 Comparative Example 8 58.7±0.6 65.8±2.1 Comparative Example 9 58.9±1.8 67.0±0.7 Comparative Example 10 68.3±1.4 79.0±0.6 Comparative Example 11 62.2±1.1 67.6±1.2 Comparative Example 12 60.8±0.7 64.0±1.4 Comparative Example 13 43.9±1.1 47.8±0.7 Comparative Example 14 42.3±1.2 48.0±0.5

According to this, it was confirmed that the culture prepared according to Example 2 of the present invention has a very excellent collagen production promoting effect compared to the untreated group and comparative examples. On the other hand, it was confirmed that a culture using one strain or a culture cultured without using three kinds of complex herbal medicines exhibited a relatively low collagen production ability compared to Example 2. In addition, in the case of Comparative Example 2 containing Asan hot spring water, not Dogo hot spring water, and Comparative Example 4 containing Onyang hot spring water, including three kinds of composite herbal medicines, the collagen production ability was higher than that of other comparative examples, but in Example 2 In comparison, it showed a relatively low collagen production ability.

Experimental Example 4: Analysis of tyrosinase inhibitory activity

The tyrosinase inhibitory ability of the cultures of Examples and Comparative Examples was measured by the method of Yagi et al. (1986). Melanin exists in the basal layer of the epidermis, and melanin synthesis is regulated by tyrosinase in melanocyte intracellular melanocytes. Therefore, inhibiting tyrosinase activity can reduce melanin synthesis and pigmentation. 0.2 ml of 110 Unit/ml mushroom tyrosinase was added to a mixture containing 0.2 ml of a substrate solution in which 10 mM L-DOPA was dissolved in 0.5 ml of 1.15M sodium phosphate buffer at pH 6.8 and 0.1 ml of a sample solution in a test tube. After reacting at 25 ° C. for 2 minutes, the absorbance of DOPA chrome generated in the reaction solution was measured at 490 nm using a microplate reader. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added and non-added groups, and the results are shown in Table 7 below.

division Treatment concentration (% (w/v)) 50 100 Example 1 3.3±0.9 6.4±1.3 Example 2 7.9±0.7 13.7±1.0 Comparative Example 1 3.1±0.8 8.5±1.7 Comparative Example 2 6.5±0.2 11.5±0.4 Comparative Example 3 3.8±0.4 8.7±0.7 Comparative Example 4 6.1±0.6 10.9±0.6 Comparative Example 5 2.1±0.7 6.8±0.7 Comparative Example 6 3.2±0.4 8.1±0.3 Comparative Example 7 3.3±0.2 6.7±0.1 Comparative Example 8 2.4±0.3 6.8±1.1 Comparative Example 9 2.9±0.9 5.9±0.6 Comparative Example 10 4.8±0.2 8.6±0.1 Comparative Example 11 4.0±0.4 8.6±0.3 Comparative Example 12 4.2±0.7 8.4±0.6 Comparative Example 13 3.8±0.2 6.7±0.8 Comparative Example 14 3.6±0.4 7.1±0.5

According to this, the culture of Example 2 of the present invention was found to have the highest tyrosinase inhibitory activity, and Comparative Example 2 containing Asan hot spring water, not Dogo hot spring water, including three kinds of complex herbal medicines, including Onyang hot spring water In the case of Comparative Example 4, the tyrosinase inhibitory activity was higher than that of other Comparative Examples, but was measured at a relatively low level compared to Example 2. On the other hand, when only one fermentation strain was used or two or less herbal medicinal materials were used, the tyrosinase inhibitory activity was measured to be relatively low.

Experimental Example 5: Analysis of melanin production inhibitory ability

Mouse-derived melanocyte B16F1 cells produce melanin, and melanin is biosynthesized in the melanosome, an organelle within the melanocyte present in the basal layer of the epidermis, and the amount of melanin produced can be compared by measuring the absorbance in the visible light range. After inoculation of B16F1 cells, the sample was treated for 3 days, the medium was removed, the cells were washed with PBS, 1 ml of 1 N NaOH was added to each well, and the melanin component was dissolved by stirring to melt the cell membrane, and the visible light range The amount of melanin produced was measured by measuring the absorbance at 400 nm, which is the shortest wavelength in the blue visible ray region.

The amount of melanin was measured by a method of expressing absorbance per unit cell number (10 ⁶ cell), and the relative amount of melanin production with respect to the control group (untreated group) was calculated as an inhibition rate (%), and the results are summarized in Table 8.

Classification (unit: %) Concentration (μg/ml) 50 100 untreated 40.5±0.6 Example 1 46.7±0.4 53.7±0.4 Example 2 68.2±0.8 75.5±0.9 Comparative Example 1 44.5±0.4 47.7±0.2 Comparative Example 2 60.0±1.2 71.2±0.8 Comparative Example 3 43.7±0.9 48.5±1.0 Comparative Example 4 61.4±0.5 72.1±0.2 Comparative Example 5 36.2±0.9 45.7±0.6 Comparative Example 6 42.0±0.2 48.7±0.4 Comparative Example 7 45.0±0.7 53.6±0.9 Comparative Example 8 42.1±1.2 49.3±0.4 Comparative Example 9 48.0±1.1 48.7±1.2 Comparative Example 10 50.1±0.8 58.6±0.9 Comparative Example 11 42.1±0.5 49.3±0.2 Comparative Example 12 42.8±0.5 48.6±0.6 Comparative Example 13 38.9±0.9 47.7±0.5 Comparative Example 14 36.8±0.4 49.1±0.5

According to this, the culture of Example 2 was found to have the best melanin production inhibitory ability, and in Comparative Example 2 containing Asan hot spring water, not Dogo hot spring water, including three complex herbal medicines, and Comparative Example 4 containing Onyang hot spring water In this case, the melanin production inhibitory ability showed a high level compared to other comparative examples, but was measured relatively low compared to Example 2. In addition, it was found that the ability to inhibit melanogenesis was greatly reduced when only one fermented strain was used or two or less herbal medicines were used.

Formulation examples of cosmetic compositions suitable for application of the present invention are presented.

Formulation Example 1: Lotion

Formulation examples of lotion using the cosmetic composition of Example 2 are shown in Table 9 below.

ingredient Content (% by weight) Composition of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 2: Lotion

Formulation examples of lotions using the cosmetic composition of Example 2 are shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 2 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadamia nut oil 2.0 polysorbate 60 1.5 Sorbitan sesquioleate 1.0 carboxyvinyl polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 3: BB Cream

Formulation examples of BB cream using the cosmetic composition of Example 2 are shown in Table 11 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Pro-type glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 4: Essence

Formulation examples of essence using the cosmetic composition of Example 2 are shown in Table 12 below.

ingredient Content (% by weight) Composition of Example 2 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 8.0 carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 5: Emulsion for mask pack

Formulation examples of emulsions for mask packs using the cosmetic composition of Example 2 are shown in Table 13 below.

ingredient Content (% by weight) Composition of Example 2 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Although the embodiments of the present invention have been described above, those skilled in the art can add, change, delete, or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention can be variously modified and changed by the like, and this will also be said to be included within the scope of the present invention.

Claims (9)

Cultures containing strains of the genus Lactobacillus cultured in a medium containing Dogo hot spring water; and
Bifidobacterium cultured in a medium containing Dogo hot spring water ( Bifidobacterium ) A culture containing a genus strain; A cosmetic composition containing as an active ingredient. According to claim 1,
The medium containing the Dogo hot spring water is a cosmetic composition, characterized in that it further comprises a herbal extract containing at least one selected from the mudanpi extract, gyejochuk extract and yeongyo extract. According to claim 2,
The herbal extract is a cosmetic composition, characterized in that extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. According to claim 2,
The medium containing the Dogo hot spring water is a cosmetic composition, characterized in that it contains 10 to 40% (v / v) of the Dogo hot spring water. According to claim 2,
The medium containing the Dogo hot spring water contains 1 to 5% (v / v) of the herbal extract, and the herbal extract has a solid content of 10 to 20% (w / v) Cosmetic composition. According to claim 1,
The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) A cosmetic composition comprising 1.0 to 2.0 mg/L. According to claim 1,
The strains of the genus Lactobacillus include Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus harbinensis, Lactobacillus casei, Lactobacillus sake ( Among Lactobacillus sakei), Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus rhamnosus A cosmetic composition, characterized in that the selected one. According to claim 1,
The Bifidobacterium genus strains include Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium lactis, and Bifidobacterium bifidum. (Bifidobacterium bifidum) A cosmetic composition, characterized in that any one selected from. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that for skin whitening or wrinkle improvement.