KR101873244B1 - Skin composition comprising the sprouting seed extract of Schisandra chinensis for anti-wrinkle or anti-inflammatory activity - Google Patents

Abstract

The present invention relates to a composition containing an extract of germinated omija seeds as an active ingredient, and more specifically to a DPPH radical scavenging activity inhibition experiment (Experimental Example 1); Elastase inhibitory activity inhibition experiment (Experimental Example 2); Measurement of cell viability by MTT assay (Experimental Example 3); Expression of Anti-Aging-Related Protein ( MMP- 1) in Cells Experimental example 4); Tyrosinase inhibitory activity experiment (Experimental Example 5); Cellular tyrosinase inhibitory activity experiment (Experimental Example 6); nitric oxide (NO) production test (Experimental Example 7), and the like, it can be usefully used as a composition for treating wrinkles and treating and preventing skin inflammatory diseases.

Description

[0001] The present invention relates to a skin-wrinkle-preventing and anti-inflammatory composition containing an extract of gingival omija as an active ingredient,

The present invention relates to a skin wrinkle preventing and anti-inflammatory composition containing an extract of germinated omija seed as an active ingredient.

[Document 1] 1. Toxic effects of ultraviolet radiation on the skin. Matsumura Y., H.N. Ananthaswamy. Toxicol. Appl. Pharmacol. Mar 15; 195 (3), 298-308, 2004

[Document 2] Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. Bernstein E. F., Y. Q. Chen, K. Tamai, K. J. Shepley, K.S. Resnik, H. Zhang, R. Tuan, A. Mauviel and J. Uitto. J. Inverst. Dermatol. 103 (2), 182-186, 1994

[Literature 3] Skin aging andphotoaging: anoverview. Gilchrest B. A. J Am Acad Dermatol. Sep; 21 (3Pt2): 610-613, 1989

[4] The Free Radical Theory of Aging. Harman D. Antioxidants & Redox Signaling. October.5 (5), 557-561, 2003

[Literature 5] Experimental study on the effect of several herbs on collagen synthesis. Yoon J. E., Kim N. K., Hwang C. Y., Jo E. H., Park M. C. J. Oriental medical ophthalmology & Otolaryngology & Dermatology. 23 (3), 33-41,2010

[Literature 6] Effect of Water Extract in Fruits of Omija (Schizandra chinensis Baillon) on CCI4 Toxicity. Lee J. S., Lee S. W. Korean J. DIETARY CULTURE 5 (2), 1990

[Literature 7] A study on Making jelly with omija extract, Kim J. E., Jeon H.J. ENGLISH J. SOC. FOOD SCI.6 (3), 1990

[Literature 8] Studies on Antioxidative Effects of Schizandra chinensis Seed Etract, Choi. H. S., Beik. K. Y., Kim. J. B. J. Korean Society of Cosmetology. 18 (4) 908-905,2012

Since human skin is the outermost part of the body directly touching the outside, it is constantly subject to direct oxidation. As internal factors of aging, the hormone secretion of the body, which regulates metabolism, is decreased, and the function and activity of immune cells and skin cells are lowered, so that the biosynthesis of essential immunoglobulin and essential proteins is reduced, There are aging and outsiders are pollution such as pollution, wind, temperature and photoaging by ultraviolet ray. This aging process results in skin cell damage, pigmentation, wrinkles, decreased elasticity, increased dryness of the skin, as well as loss of direct function of the skin's major components, leading to spots, freckles and blotches (1-4) .

Recently, interest in and research on various herbal medicine and edible plant extracts containing a large amount of physiologically active substances with few side effects and harmful effects on the human body has been actively promoted as the recognition of natural ingredients derived from plants has been increasing. And bio and cosmetics.

Omija is a deciduous woody plant belonging to Magnoliaceae and has five flavors: sweet, sour, sincerity, gummy, and stern (5,6). It has been reported that Omija has the role of regulating the non-physiological action of the body and the function of protecting the lungs and kidneys, and is effective for heart, liver, hunger, and noise. Studies have been made that omija can be used as a skin improving substance as a cosmetic material. Various researches on Omiza fruit itself have been reported, but there is not much research as a cosmetic material using Seed extract and Omiza seed extract separately.

Korean Patent Laid-Open Publication No. 10-2013-0087938 (published on Aug. 07, 2013) discloses a cosmetic composition for improving wrinkles containing an extract of Omija; Korean Patent Publication No. 2002-0037968 (published on May 23, 2002) discloses antioxidant and antibacterial effects of Omija seed extract and fractions; Korean Patent No. 10-2015-0067597 (published Jun. 18, 2015) discloses a composition for promoting muscle antioxidation and immunological activity including an extract of Omija seed, a method for producing the same, and the like.

However, none of the above documents mention or disclose the therapeutic effect of wrinkle-reducing extracts on wrinkles and skin inflammatory diseases.

The inventors of the present invention conducted experiments for inhibiting DPPH radical scavenging activity (Experimental Example 1) on the extract of the present invention; Elastase inhibitory activity inhibition experiment (Experimental Example 2); Measurement of cell viability by MTT assay (Experimental Example 3); Expression of Anti-Aging-Related Protein (MMP-1) in Cells Experimental example 4); Tyrosinase inhibitory activity experiment (Experimental Example 5); Cellular tyrosinase inhibitory activity experiment (Experimental Example 6); nitric oxide (NO) production test (Experimental Example 7), and the like. Thus, the present invention was completed.

In order to achieve the above object, the present invention provides a dermatological pharmaceutical composition for improving wrinkles and treating and preventing skin inflammatory diseases, which comprises an extract of germinating omija as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for improving wrinkles and prevention and improvement of skin inflammatory diseases, which comprises an extract of gingival Omija seed as an active ingredient.

The germinating omija seeds, as defined herein, are prepared by seeding Omija seeds at a relative humidity ranging from about 20 to 90%, preferably 40 to 80%, more preferably 50 to 70%, 0 to 15 캜, preferably 2 to 10 Culturing at a culture temperature of 10 to 35 占 폚, preferably 15 to 30 占 폚 under a fermentation period of 6 hours to 72 hours, preferably 12 hours to 36 hours, under a dark place at a temperature of 3 to 7 占 폚, preferably 3 to 7 占 폚 And germinated omija seed germinated 1 to 14 days, preferably 3 to 7 days after germination.

The extracts as defined herein include germinating omija seed extracts and specifically include water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, propylene glycol, , Functional glycerin, preferably water or water and ethanol mixed solvent, most preferably 60% to 80% ethanol soluble extract.

Inflammatory skin diseases as defined herein include inflammatory dermatoses, psoriasis, wounds, atopic diseases, preferably psoriasis, wounds, atopic diseases, inflammatory dermatitis, more preferably atopic diseases or inflammatory dermatitis.

The pharmaceutical composition for external application for skin, which comprises the gingival extract as an active ingredient, is contained in an amount of 0.1 to 50% by weight based on the total weight of the composition.

The pharmaceutical composition includes a cream, a gel, a patch, a spray, an ointment, an alarm, a lotion, a liniment, a pasta or a cataplasmal formulation.

In addition, the cosmetic composition includes formulations of lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, and pack.

Specifically, a method for producing the extract of the present invention will be described.

For example, water having a volume of about 1 to 100 times, preferably about 2 to 20 times the weight of the germinating omija seed of the present invention, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, butylene glycol, At least one solvent selected from the group consisting of propylene glycol, hydrolyzed butylene glycol, water-containing propylene glycol and water-containing glycerin, preferably water or water and ethanol mixed solvent, most preferably 60% to 80% , Preferably 30 to 80 ° C for about 1 to 14 days, preferably 2 to 6 days, using an extraction method such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, preferably by cold extraction A second step of repeating the extraction method; The extract of the present invention can be obtained through filtration of the extract solution, classification into a supernatant and a precipitate, and a third step of filtrating and concentrating the supernatant and the precipitate, respectively.

The present invention provides a cosmetic composition for external use and a cosmetic composition having a wrinkle-improving and anti-inflammatory effect containing the gauze omija seed extract obtained by the above-mentioned production method and the above-mentioned production method as an active ingredient.

The present inventors conducted experiments for inhibiting DPPH radical scavenging activity (Experimental Example 1) on the extract of the present invention; Elastase inhibitory activity inhibition experiment (Experimental Example 2); Measurement of cell viability by MTT assay (Experimental Example 3); Expression of Anti-Aging-Related Protein (MMP-1) in Cells Experimental example 4); Tyrosinase inhibitory activity experiment (Experimental Example 5); Cellular tyrosinase inhibitory activity experiment (Experimental Example 6); nitric oxide (NO) production test (Experimental Example 7), it was confirmed that it is useful as a composition for treating wrinkles and treating and preventing skin inflammatory diseases.

In addition, Omija has been used for herbal medicine and food for a long time, and the extract of the present invention extracted therefrom has no problems such as toxicity and side effects, and since it has proved to be a non-irritant sample in skin patch test, have.

The dermatological pharmaceutical composition containing the extract of the present invention can be used as a pharmaceutical composition in the form of external preparation for skin such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma However, the present invention is not limited thereto.

The preferred dosage of the extract of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at a daily dose of 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The extract of the present invention can be used variously in cosmetics and cleansers having a whitening effect.

Examples of products to which the present composition can be added include cosmetics such as lotion, skin, lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, cleansing, cleanser, soap, have.

The cosmetic composition of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , Derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbin, dl-alpha tocopherol acetic acid, dl-alpha tocopherol nicotinic acid vitamin E, dl-pantothenyl alcohol, D-pantothenyl alcohol, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may be blended with other essential ingredients, if necessary, in combination with the essential ingredients.

Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo And monohydroxystearic acid and esters such as sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE (Polyoxyethylene / polyoxypropylene) copolymer, POE.POP alkyl ether, polyether-modified silicone, polyether-modified silicone, polyoxyethylene-polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl-1'- , 3,5-triazine, 2- (2- And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range not to impair the objects and effects of the present invention, but it is preferably 0.01 to 5% by weight based on the total weight, Preferably 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned fermented bean curd extract, for example, stabilizers, solubilizers, vitamins, pigments, Phosphorus auxiliaries and carriers.

The cosmetic composition of the present invention can be prepared into any formulation conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, and hair cosmetics.

Specifically, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The germinating omija extract of the present invention was tested for inhibition of DPPH radical scavenging activity (Experimental Example 1); Elastase inhibitory activity inhibition experiment (Experimental Example 2); Measurement of cell viability by MTT assay (Experimental Example 3); Expression of Anti-Aging-Related Protein ( MMP- 1) in Cells Experimental example 4); Tyrosinase inhibitory activity experiment (Experimental Example 5); Cellular tyrosinase inhibitory activity experiment (Experimental Example 6); nitric oxide (NO) production test (Experimental Example 7), and the like, it can be usefully used as a composition for treating wrinkles and treating and preventing skin inflammatory diseases.

1 is a graph showing the elastase inhibitory activity of the sample of the present invention

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) ± SD of triplicate);
2 is a graph showing the effect of the present invention sample on the cell survival rate of CCD986sk fibroblast cells (

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) ± SD of triplicate);
FIG. 3 is a graph showing MMP-1 inhibitory activity of human dermal fibroblast of the present invention sample (cultured only with a control cell carrier,

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) ± SD of triplicate);
Fig. 4 is a graph showing tyrosinase inhibitory activity of B16F10 melanoma cells of the present invention sample (

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) ± SD of triplicate);
FIG. 5 is a graph showing the intracellular tyrosinase inhibitory activity of B16F10 melanoma cells of the present invention sample (

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) ± SD of triplicate);
6 is a graph showing the effect of the present invention sample on the production of nitrogen oxides (NO) (

SCE: 70% EtOH extracted Schisandra chinensis ;

G-SCE: 70% EtOH extracted Germinated Schisandra chinensis;

EGCG: Epigallocatechin gallate, respectively, and the results are expressed as mean (means) SD of triplicate).

Below, The present invention will be described in detail by the following examples and experimental examples.

However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Comparative Example 1. Preparation of Omija seed extract

The Omija seeds used in this experiment were supplied from Samcheongbong Farm, Gimcheon (251-3, Heinri, Bukhyeon, Gyeongbuk). 100 g of Omija was added to 1 L of 70% ethanol, and the mixture was immersed and extracted at room temperature for 24 hours, and then repeatedly filtered three times with a filter paper (Whatman No. 1). The filtered extract was concentrated and then lyophilized and stored in a refrigerated room in powder form (hereinafter referred to as "SCE").

Example 1 Preparation of gingival Omija seed extract

The germinated omija seeds used in this experiment were supplied and used in Gimcheon Samdongbong Farm.

Specifically, germination omiza seeds were used as a germinated omija seed which was cultured at 60% humidity at 5 ° C for 24 hours at 20 to 25 ° C, and germination was started for 3 to 7 days.

100 g of the dried germinating omija seeds was added to 1 L of 70% ethanol, and the mixture was immersed and extracted at room temperature for 24 hours, and then repeatedly filtered three times with a filter paper (Whatman No. 1). Each extract was concentrated and then lyophilized and stored in a refrigerated room in powder form (hereinafter referred to as "G-SCE").

Experimental Example 1 Measurement of Elastase Inhibitory Activity

In order to test the elastase inhibitory activity of the samples obtained in the examples, Cannell et al. (Cannell RJP, Kellan SJ, Owsians AM and Walker JM. (1988) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Media . 54 (1), 10-14].

N-succinyl- (L-Ala) 3-Aster glehni-nitroanilide was used as a substrate and the amount of Aster glehni-nitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 405 nm. Each test solution was prepared in a test tube at a constant concentration of 0.5 mL. The test tube was washed with porcine pancreas elastase (2.5 U / mL, Sigma Chemical Co., St. Louis, Mo., USA) dissolved in 50 mM tris-HCl buffer (pH 8.6) ) Was added and 0.5 ml of N-succinyl- (L-Ala) 3-Aster glehni-nitroanilide (0.5 mg / ml) dissolved in 50 mM tris-HCl buffer (pH 8.6) Respectively. Elastase inhibitory activity was expressed as the absorbance of the sample solution and the absorbance of the sample without addition.

Elastase is an enzyme that degrades elastin, an important substrate for maintaining skin elasticity in the dermis. Elastase is also a nonspecific hydrolytic enzyme capable of degrading collagen, another important substrate protein. Thus, the elastase inhibitor has the function of improving the wrinkles of the skin, and ursolic acid has been used as the elastase inhibitor. Elastase is known to be the main cause of wrinkle formation by decomposing elastin, the insoluble elastic fiber protein of animal connective tissues, and breaking the network structure of the dermis early in the skin. In the dermis of the skin, collagen and elastin related to the elasticity of the skin form a network structure. As the network structure is broken, that is, the elastin is decomposed by the elastase, and the skin is sagged and wrinkled. Therefore, skin aging can be suppressed by lowering the activity of elastase, elastin degrading enzyme, which is one of the major causes of skin aging. Currently, studies to prevent skin aging have been actively conducted, and up to now, there have been many studies on α-1-proteinase inhibitor, mucus proteinase inhibitor, α-2-macri globulin, inter-α-trypsin, bowman-birk inhibitor, verapamil, beta lactam, chondroitin sulfates , deoxycycline, heparin, and other elastase inhibitors have been reported.

The results of measuring the elastase inhibitory activity associated with wrinkle formation are shown in FIG. In the case of Omija Seed Extract, the inhibition rate was 4.5, 32.4, 64.2 and 81.3% for the concentration of 1, 10, 100 and 1000 μg / ml, and the inhibition of elastase was 11.4, 39.7, 70.9 and 88.7% Efficacy. The antioxidant activity of germination seed extract was lower than that of Omija seed extract, but the elastase inhibitory activity was about 10% higher.

Experimental Example 3. Measurement of cell viability by MTT assay

Cell viability was measured according to the method of Carmichael et al. [3] (Carmichael, J., WG DeGraff, AF Gazdar, JD Minna, and JB Mitchell, 1987. Evaluation of a tetrazolium based colorimetric assay: assessment of chemosen- Testing. Cancer Res., 47 , 936-942.)

Each cell line (melanoma (B16F10), fibroblast (CCD-986sk), and macrophage (Raw 264.7) cells) was dispensed in a 96-well plate at a concentration of 0.6 to 8 × 10 ³ cells / well in an amount of 0.18 ml. ml and incubated at 37 ° C in a 5% CO ₂ incubator for 24 hours. For the control, add the same amount of distilled water as the sample and incubate under the same conditions. After adding 0.02 ml of MTT solution at a concentration of 5 mg / ml, the cells were incubated for 4 hours. After removing the culture medium, 0.15 ml of DMSO: Ethanol (1: 1) was added to each well and incubated at room temperature for 30 minutes. Absorbance is measured at 550 nm. The cell viability was measured by the absorbance decreasing rate of the sample solution addition group and no-addition group as shown in the following equation (1).

MTT detection method is widely used as a cytotoxicity and cell proliferation detection method because many samples can be easily read using an ELISA reader using a 96-well plate, and the dehydrogenase activity of mitochondria in cell metabolism To reduce the yellow water-soluble MTT tetrazolium to purple colored water-insoluble MTT formazan.

As a result of measuring the cell survival rate of Omija seed and germinating omija seed to CCD986sk cells, the germinating seedlings extract showed a cell viability of 80% or more at a concentration of 20 μg / ml (FIG. 2). Based on the cell survival rate, the extracts of Omija seed and germination seeds were tested for procollagen biosynthesis and MMP-1 activity at a concentration of 25 μg / ml.

Experimental Example 3. Measurement of MMP-1 Inhibitory Activity

(JY Bae, JS Choi, YJ Choi, SY Shin, SW Kang, SJ Kim, JK, Kim, YJ) Han, YH Kang 2008. (-) Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts: Involvement of mitogen-activated protein kinase. Food ChemToxicol .46,1298-1307)

The amount of Matrixmetalloprotease-1 (MMP-1) protein associated with whitening was measured to examine the anti-aging effect of the extract of the Examples. Cells were collected by centrifugation, and cell lysate was prepared by using RIPA buffer. The cells were cultured in a culture dish using a fibroblast (CCD-986sk), followed by sample treatment, followed by washing with PBS for 24 hours. The amount of protein contained in the cell lysate was measured by the Bradford method. 20 ug of protein was separated by SDS-PAGE containing 10% polyacrylamide and electroblotted with PVDF membrane at 120V for 1 hour. The PVDF membrane was then incubated in 5% skim milk for 1 hour and then overnight at 4 ° C in primary antibody. After washing three times with TBST, secondary antibody was reacted at room temperature for 1 hour, washed three times with TBST, and exposed to X-ray film by reaction with ECL kit (Amersham-Pharmacia). The bands developed on the X-ray film were quantified using Biorad gel doc.

The most affected factor for senescence-related MAPK is c-fos, which is affected by p38. Activation of these factors strongly regulates the expression of MMPs. In this study, protein expression of MMP-1 was measured in the MMP family.

Cells are inoculated on a 96-well plate at a concentration of 1 × 10 ⁴ cells / well, and the samples are added to each well and cultured in a CO ₂ incubator for 24 hours. The cultures of the cells thus obtained are collected and used for the experiment. MMP-1 was measured using Amersham matrix metallproteinase-1, Human, and Biotrak ELISA system (GE Healthcare).

Among several kinds of MMPs produced in the body, MMP-1 is a collagen-specific protease that inhibits the activity of MMP-1 to reduce collagen degradation, thereby maintaining skin elasticity and preventing wrinkle formation (Fisher GJ, Talwar HS, Lin J, Lin P, McPhillips F, Wang Z, Li X, Wang Y, Kang S, Voorhees J J. 1998. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs followed by activation of mitogen-activated protein kinase pathways in human skin in vivo . J Clin Invest. 101, 1432-1440.).

As a result of the above experiment, the results of measurement of MMP-1 inhibitory activity of the extracts of Omija and germination were shown in Fig. As a result, the expression of MMP-1 was 54.7% at a concentration of 20 μg / ml in the germinated seedlings of Seaweed extract, and the expression of MMP-1 was about 30% lower than that of Seed extract of Omija, Respectively.

Experimental Example 4. Tyrosinase inhibitory activity

In order to test the tyrosinase inhibitory activity of the samples obtained in the examples, Yagi et al. (Yagi A, Kanbara T and Morinobu N. (1986) applied the method of Yagi et al. aloe Planta Media . 3981, 517-519.]

To the reaction mixture, 0.2 mL of mushroom tyrosinase (110 U / mL) was added to a mixture of 0.2 mL of the substrate solution in which 10 mM L-DOPA was dissolved in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and 0.1 mL of the sample solution, DOPA chrome produced in the reaction solution was measured at 475 nm for 2 minutes. The tyrosinase inhibitory activity was expressed by the absorbance reduction rate of the sample solution of the following formula (2) and the no-added sample.

Melanin, a key determinant of skin tone, is biosynthesized in melanosomes in pigmented cells called melanocytes in the epidermal basal layer. The starting material for the synthesis of melanin is tyrosine, an amino acid. Tyrosine is oxidized by tyrosinase in melanocytes to L-3,4-dihydroxyl phenylalanine (DOPA) and DOPA quinone. It is known that DOPA quinone then becomes DOPA chrome, 5,6-dihydroxyindole, and indole-5,6-quinone, and then melanin is formed by polymerization with indole-5,6-quinone. (HJ and Kim, YG (2012) Effect of Salviae miltiorrhizae Radix on neuronal apoptosis following intracerebral hemorrhage of rats. Kor J Herbology 27, 89-94)

In addition, tyrosinase plays an important role in the formation of melanin in the skin. Tyrosine hydoxylase, which oxidizes tyrosine in melanosome to form DOPA, acts as a DOPA oxidase that oxidizes DOPA to form DOPA quinone. .

As a result of this experiment, the result of measurement of inhibition activity of tyrosinase derived from mushroom in order to measure tyrosinase inhibitory activity which can effectively inhibit melanin polymer biosynthesis in the skin is shown in FIG. The results showed that tyrosinase inhibition was less than 10% in all concentration ranges of 1, 10, 100, and 1000 μg / ml for Omija seed extract, and 34.7% for tyrosinase Respectively. These results showed that the whitening activity of the germinated seeds extract of Omija seed was 3 times higher than that of Omija seed extract.

Experimental Example 5. Measurement of intracellular tyrosinase inhibitory activity

In order to test cellular intracellular tyrosinase inhibitory activity of the samples obtained in the examples, the following method was applied as described in the literature (Akiu S, Suzuki Y, Asahara T, Fujinuma Y and Fukuda M. (1991) Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells. Nippon Hifuka Gakkai Zasshi . 101 (6), 609-613).

Melanoma (B16F10 ATCC) cells were inoculated into each culture dish, and the medium was removed and washed with PBS. Lysis buffer (67 mM sodium phosphate buffer, 1% triton X-100, 0.1 mM phenylmethyl sulfonyfluoride) After incubation for 1 hour and centrifugation at 13,200 rpm for 20 minutes, the supernatant is used as the enzyme solution, which is dissolved in 67 mM phosphate buffer 120ul of 8.0mM L-DOPA dissolved in pH 6.8 and 40ul of the sample solution were added to a 96-well plate, and 40ul of the enzyme solution dissolved in 67mM phosphate buffer (pH 6.8) was added and incubated at 37 ° C for 30 minutes , And the amount of generated DOPA chrome was measured at 490 nm. The inhibition rate was calculated by the following formula (3).

In order to measure the intracellular tyrosinase activity, tyrosinase activity was measured by treating Omija seed and germinating omija seed extract with B16F10 melanoma cells, and the result was shown in Fig. Seed extracts of Omija seed and germination seed showed 87 and 72% tyrosinase expression at 20 μg / ml, respectively. Seeds of germinating omija showed a tyrosinase inhibitory effect similar to that of 30%.

Experimental Example  6. Nitric oxide measurement

In order to examine the inhibitory effect on the nitric oxide (NO) and proinflammatory cytokine production amount of the sample obtained in the above examples, the following experiment was performed using the method described in the existing literature (Yang JH et al., Anti- inflammatory activity of ethylacetate fraction of Cliona celata, Immunopharmacol Immunotoxicol 2011 , 33 (2), 373-379).

RAW 264.7 cells ( ⁵ × 10 ⁵ cells / well were preincubated in a 24-well plate, and DMEM medium was used to pre-treat for 10 minutes at a concentration of 6.25, 12.5, 25 and 50 μg / ml. And cultured at 37 ° C and 5% CO ₂ . After incubation for 24 hours, the supernatant was collected and reacted with 100 μl of the same amount of Griess reagent (G4410, Sigma). The absorbance was measured at 540 nm using an ELISA reader (Sunrise-Basic Tecan, TECAN) The amount of NO produced is shown graphically using a standard curve. The level of proinflammatory cytokine (TNF-α) was measured by ELISA kit (R & D System, USA).

As a result of the above experiment, in order to measure the degree of inhibition of NO production in Raw264.7 cells, the amount of NO produced by treating the cells of Omija seed and germinated omija seed extract with different concentrations was measured as shown in Fig. The LPS-treated group showed a higher level of NO expression than the LPS-untreated group, and it was confirmed that NO expression was decreased by each extract concentration. In particular, germination of Omija seed extract showed a 90% inhibition rate at a concentration of 25 μM. Inhibition of inflammation in macrophage cell line was highly inhibited by germinating seed germ extract.

Statistical processing

Experimental results were expressed as mean ± SD after 3 repeated measurements. Statistical analysis was performed using a SAS program (SAS Institue, Cary, NC, USA). Duncan's multiple test was performed and the correlation was analyzed.

Hereinafter, formulations of cream, massage cream, lotion, skin lotion, essence, pack, and cleansing foam are exemplified as the formulation examples of the present invention, but the formulations including the cosmetic composition of the present invention are not limited thereto.

Formulation Example  1. Cream composition

The oil phase and water phase are heated to 75 ° C and cooled to room temperature.

Formulation Example  2. Massage Cream  Composition

The oil phase and water phase are mixed by heating at 75 DEG C and then cooled to room temperature.

Formulation Example  3. lotion composition

The oil phase and water phase are mixed and emulsified by heating at 75 ° C and then cooled to room temperature.

Formulation Example  4. Skin lotion composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  5. Essence composition

The water phase and the ethanol phase are respectively prepared and mixed and then filtered.

Formulation Example  6. Pack composition

The water phase and the ethanol phase are dispersively dissolved and mixed, and then cooled to room temperature.

Formulation Example  7. Cleansing Foam  Composition

The water phase and the oil phase are dispersed and dissolved, mixed and sieved, and then cooled to room temperature.

Claims (6)

Cultured at a culture temperature of 10 to 35 ° C under a fermentation period of 6 hours to 72 hours under a darkness of 0 to 15 ° C at a relative humidity in the range of 20 to 90%, and germinated at a temperature of 1 to 14 days An extract of the present invention which is soluble in water or a mixed solvent of water and ethanol as an active ingredient for the treatment and prevention of inflammatory dermatitis, psoriasis, wound, or atopic disease. delete delete delete Cultured at a culture temperature of 10 to 35 ° C under a fermentation period of 6 hours to 72 hours under a darkness of 0 to 15 ° C at a relative humidity ranging from 20 to 90%, and germinated at a temperature of 1 to 14 days A cosmetic composition for improving and preventing inflammatory dermatitis, psoriasis, wounds, or atopic diseases, which contains extracts soluble in water or a mixed solvent of water and ethanol as an active ingredient. delete

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