KR102079263B1 - Method for refining horse oil and Cosmetic composition using refined horse oil manufactured therefrom - Google Patents

Abstract

The present invention relates to a method for purifying horse oil, and a cosmetic composition using the purified horse oil obtained therefrom. More particularly, the present invention relates to a method for purifying horse oil, capable of obtaining purified horse oil which has a low peroxide value and acid value, and is colorless and odorless, and to a cosmetic composition using the purified horse oil obtained therefrom. The cosmetic composition according to the present invention includes any one or more selected from the group consisting of a Phyllanthus urinaria extract, Ilex crenata extract, daffodil extract, Ligustrum obtusifolium extract, and Deutzia parviflora extract.

Description

Method for refining horse oil and Cosmetic composition using refined horse oil manufactured therefrom

The present invention relates to a method for purifying horse oil and a cosmetic composition using purified horse oil, and more particularly, a method for purifying horse oil, which has a low peroxide value and an acid value, to obtain a colorless and odorless refined horse oil, and a tablet obtained therefrom. It relates to a cosmetic composition using horse oil.

Horse oil (Horse oil) is a material that can supply unsaturated fatty acid to the skin because it has high affinity with human skin and contains more than 60% of unsaturated fatty acid because the balance of sebum and fatty acid of human oil, oil and water are very similar. It is known that oleic acid, which has a moisturizing effect, linoleic acid having an anti-atopic effect, and palmitoleic acid, which has an effect of promoting cell regeneration, has an anti-atopic effect and an effect of restoring skin barrier during burn. Therefore, a manufacturing method of horse oil for use in various types of cosmetics and cosmetics containing horse oil as described in the following patent document has been developed.

<Patent Documents>

1. Korean Patent Publication No. 10-2018-0026508 (published Mar. 12, 2018) "Method for purifying horse oil using a filter containing citrus fruits as a filler and refined horse oil prepared by the method"

2. Publication No. 10-2018-0030287 (published Mar. 22, 2018) "Cosmetic composition comprising a nano emulsion containing horse oil or camellia oil"

However, conventionally, there is a problem in that it is impossible to obtain high-quality refined horse oil having a low acid value and a peroxide value, focusing only on the deodorizing effect of horse oil.

The present invention is to solve the above problems of the prior art,

It is an object of the present invention to provide a method for purifying horse oil which can obtain refined horse oil of improved quality by optimizing the purification conditions.

Another object of the present invention is to provide a method for purifying horse oil, which has a low peroxide value and an acid value and can obtain colorless and odorless refined horse oil.

The present invention is implemented by the embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the method for purifying horse oil according to the present invention is a crude oil extraction step of extracting the crude oil from which impurities are removed by adding hot water to the raw material and filtering the crude oil, acid value and It is characterized in that it comprises a crude oil refinement step of adjusting the peroxide value and obtaining a colorless and odorless refined horse oil.

According to another embodiment of the present invention, in the method of purifying horse oil according to the present invention, the crude oil refining step is 100 parts by weight of crude oil, 1.5 to 2.5 parts by weight of dalsorb, 1.5 to 2.5 parts by weight of activated clay, 5 to 5 parts of activated carbon. 7 parts by weight, and 0.1 to 0.2 parts by weight of BHT to form a first dressing oil mixture and a first stirring step of stirring the first dressing oil mixture at 80 to 120 ℃ for 35 to 45 minutes, and the first stirring step After the first dressing oil mixture further mixed 4 to 6 parts by weight of celite to form a second dressing oil mixture and a second stirring step of stirring the second dressing oil mixture at 80 to 120 ℃ for 15 to 25 minutes Characterized in that.

According to another embodiment of the present invention, in the method for purifying horse oil according to the present invention, the crude oil refining method includes a step of obtaining a purified horse oil by filtering a second horse oil mixture after the second stirring step. It features.

According to another embodiment of the present invention, in the method of purifying horse oil according to the present invention, the dressing oil extracting step is a pulverizing step of grinding the dough to a certain size, and the mixture of activated carbon and diatomaceous earth A mixing step of forming a dough mixture, a hot water treatment step of encapsulating the raw dough mixture with a cloth, then immersing in water to form a water mixture, and heating the water mixture, and filtering the water mixture after the hot water treatment step It is characterized in that it comprises a filtration step to obtain, and the separation step of obtaining a crude oil by removing the water of the water layer after the separation of the filtrate obtained in the filtration step to the oil layer and the water layer.

According to another embodiment of the present invention, in the mixing method of horse oil according to the present invention, activated carbon is used in the mixing step 3 to 5 parts by weight based on 100 parts by weight of the dough, diatomaceous earth is based on 100 parts by weight of the dough 0.5 to 2 parts by weight is used, in the hot water treatment step, water is used 800 to 1000 parts by weight based on 100 parts by weight of the dough, the water mixture is heated for 50 to 70 minutes at a temperature of 85 to 95 ℃, the filtration step The water mixture is characterized in that it is carried out after cooling to 68 to 70 ℃.

According to another embodiment of the present invention, the cosmetic composition according to the present invention comprises refined horse oil, wherein the refined horse oil is to use the purified horse oil prepared by the method of any one of claims 1 to 5. It features.

According to another embodiment of the present invention, the cosmetic composition according to the present invention, in order to provide an anti-wrinkle effect, at least one selected from the group consisting of fox bead extract, Holly leaf extract, Narcissus extract, Privet extract and horse valgus extract It characterized in that it further comprises.

According to another embodiment of the present invention, the cosmetic composition according to the present invention, the cosmetic composition further comprises an extract for providing a wrinkle improving effect, the extract comprises a Holly tree extract, Privet extract and horse valgus extract And, the Quercus larvae extract, Privet extract and horse valgus extract is characterized in that it is mixed in a weight ratio of 1: 1: 1.

The present invention can achieve the following effects by the above embodiment.

The present invention has the effect of obtaining refined horse oil of improved quality by optimizing the purification conditions.

In addition, the present invention has a low peroxide value and acid value has the effect of obtaining a colorless and odorless refined horse oil.

1 is a flow chart showing a purification method of horse oil according to an embodiment of the present invention.
Figure 2 is a photograph showing the purified horse oil obtained through the purification method of horse oil according to an embodiment of the present invention.

Hereinafter will be described in detail with reference to the accompanying drawings a cosmetic composition using the method of refining horse oil according to the present invention and the purified horse oil obtained therefrom. Unless otherwise defined, all terms in this specification are equivalent to the general meaning of the terms understood by those of ordinary skill in the art to which the present invention pertains, and in case of conflict with the meaning of the terms used herein Follow the definition used in the specification. In addition, detailed description of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when a part is said to "include" a certain component, it means that it may further include other components, without excluding other components unless specifically stated otherwise.

1 and 2, a method of refining horse oil according to an embodiment of the present invention, the method of refining horse oil is a crude oil extract for extracting the crude oil from which impurities are removed by filtration after adding hot water to the horseshoe. Step (S1), and the crude oil refining step (S2) and the like to refine the crude oil to adjust the acid value and peroxide value to obtain a colorless and odorless refined horse oil.

The dressing oil extracting step (S1) is a step of extracting the dressing oil from which impurities are removed by adding hot water to the raw material and filtering it, a pulverizing step (S11) of crushing the raw material to a certain size, and to the ground Mixing step (S12) of mixing the activated carbon and diatomaceous earth to form a malt mixture, and after wrapping and sealing the malt mixture with a cloth to form a water mixture in water to heat the water mixture (S13) and Filtration step (S14) of filtering the water mixture after the hot water treatment step to obtain a filtrate, and separating the filtrate obtained from the filtration step into an oil layer and a water layer, and then removing water in the water layer to remove crude water ( Oil phase) to obtain a separation step (S15) and the like.

The raw material refers to a mass of fat obtained through meat in the slaughtered horse. In the mixing step, the activated carbon is preferably used 3 to 5 parts by weight based on 100 parts by weight of the dough, and the diatomaceous earth is preferably used 0.5 to 2 parts by weight based on 100 parts by weight of the dough. In the hot water treatment step, the cloth may be a nonwoven fabric, a cotton cloth, or the like, and water is preferably used in an amount of 800 to 1000 parts by weight based on 100 parts by weight of the raw material, and the water mixture is used for 50 to 70 minutes at a temperature of 85 to 95 ° C. It is preferred to be heated. The filtration step is performed by cooling the water mixture to 68 to 70 ℃, it can be carried out using a variety of known extractors, filters and the like.

The crude oil refining step (S2) is a step of purifying the crude oil to adjust the acid value and peroxide value to obtain a colorless and odorless refined horse oil, 100 parts by weight of crude oil, dalsorb (dalsorb, magnesium silicate) 1.5 to 2.5 By weight, activated clay 1.5 to 2.5 parts by weight, activated carbon 5 to 7 parts by weight, BHT (dibutyl hydroxytoluene) 0.1 to 0.2 parts by weight of the mixture to form a first dressing oil mixture and the first dressing oil mixture 80 to 120 The first stirring step (S21) to stir at 35 ℃ for 45 minutes, and after the first stirring step, additionally mixed 4 to 6 parts by weight of celite (celite) to the second dressing oil mixture Forming and stirring the second crude horse oil mixture at 80 to 120 ℃ for 15 to 25 minutes (S22), and obtaining a purified horse oil by filtering the second horse oil mixture after the second stirring step ( S23) and the like. Purified horse oil obtained through the obtaining step has a colorless and odorless as shown in FIG. 2 to maintain a liquid state at room temperature.

When the dalsorb is used less than 1.5 parts by weight can not lower the peroxide value, when the dalsorb is used more than 2.5 parts by weight the peroxide decrease is not large, but rather the acid value is increased, the celite is not used or initially The peroxide value cannot be lowered when the crude oil mixture is mixed to form the first crude oil mixture, and the peroxide value and the acid value are effectively used when activated clay and activated carbon are used together and used in a specific range (1.5 to 2.5 parts by weight of activated clay and 5 to 7 parts by weight of activated carbon). The lower the peroxide value can be lowered for a long time if the additional mixing of BHT, refined horse oil obtained through the present invention can keep the acid value low, with or without preservatives. The crude oil mixture may be stirred at a temperature of 80 to 120 ℃ to increase the yield of refined horse oil, especially when stirred at a temperature of 80 ℃ to increase the yield of refined horse oil as well as to obtain a transparent purified horse oil obtained do.

Another embodiment of the present invention relates to a cosmetic composition using purified horse oil obtained from the method of refining horse oil, wherein the cosmetic composition includes refined horse oil. The refined horse oil may be included in an amount of 1 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may include any one or more selected from the group consisting of fox bead extract, Holly leaf extract, Narcissus extract, Privet extract and Horsevalley extract, to improve skin wrinkles, Holly leaf extract, Privet extract and It is more preferable that the horse-valley extract is used in a mixture of 1: 1: 1 by weight. The extract for improving the wrinkles will be contained in an amount of 0.001 ~ 10% relative to the total weight of the cosmetic composition. The fox bead extract, Holly leaf extract, Narcissus extract, Primrose extract and horse valley extract are mixed with each of fox leaf, Holly leaf, narcissus leaf, Privet leaf, horse valgus leaf in ethanol and then remove dry The extract is concentrated under reduced pressure and freeze-dried. The fox dictation is a yearly herbaceous plant with a dicotyledonous plant, the branch has narrow wings, the leaves are alternate, and hangs in two lines, and the flower is monogamous, blooms in July to August, and has reddish brown. The Holly is an evergreen broad-leaved shrub of the dicotyledonous tree Agaricaceae, the bark is gray, the leaves are alternate, and the eggs are upside down or long oval-shaped with both teeth and edges on the edges, and the flowers are male and female. It blooms in May-June. The narcissus is a perennial herb of the monocotyledonous genus Narcissus, the scaly stem is broad egg-shaped, the bark is black, the leaves are row-shaped, and the flowers bloom in December-March. The rattan is a deciduous shrub of the dianthus genus Aspenaceae, whose branches are thin and grayish white, the leaves are opposite, long oval-shaped, 2-7cm long, dull, pointed wide, and 5 ~ flowers. It blooms white in June and runs in the form of gunshot inflorescence at the end of the branch. The horse-valley is a deciduous shrub of the dicotyledonous rose, Panaxaceae, with the young branches being virgin and the old branches are black ash-colored, the leaves are opposite, egg-shaped, egg-shaped oval or oval-shaped, flowers are white and bloom in May-June. Run on the inflorescence.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.

The water-soluble vitamins may be any compound as long as they can be blended into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H and the like. And salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamin can be obtained by a conventional method such as microbial transformation, purification from microorganism culture, enzyme method or chemical synthesis.

The oil-soluble vitamin may be any compound as long as it can be blended into cosmetics. Preferably, vitamin A, carotene, vitamin D2, vitamin D3 and vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like can be given. Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. The oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from culture of microorganisms, enzymes or chemical synthesis.

The polymer peptide may be any compound as long as it can be blended into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. The polymer peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzyme methods or chemical synthesis methods, or can be purified and used from natural products such as dermis such as pigs and cows and silk fibers of silkworms. .

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or a fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. The sphingolipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be blended into cosmetics, and preferably brown algae extract, red algae extract, green algae extract, and the like, and colorazine, arginic acid and sodium arginate purified from these seaweed extracts. And potassium arginate are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from a seaweed by a conventional method.

The cosmetic composition of the present invention can be blended with other components usually blended in the cosmetic composition. For example, fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, Cooling agent, a restriction | limiting agent, purified water, etc. are mentioned.

Examples of the fat or oil components include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils. Examples of the ester fats and oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, and isocetyl isostearate and stearic acid. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid, isetyl myristin, isostearyl mysterylate, isostaryl palmitate, octylate acid octylate acid, isocetyl isostearic acid, diethyl sebacate, adadiate Diisopropyl phosphate, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopentane Tol, Cetyl Caprylic Acid, Decyl Laurin, Hexyl Lauric Acid, Mycetic Decyl, Myristin Myristyl, Myristin Cetyl, Stearyl Stearate, Decyl Oleate, Ricinoolein Sancetyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid , 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid) , Propylene glycol dicaprylate, neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyl neopentane Dodecyl, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearic isostearic acid Reel, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myritic lactate, cetyl lactate, octyl decate, Triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, diethyl 2-ethylhexyl succinate, diisobutyl adipicate, diisocebacinate Propyl, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearic acid, phytic oleate Reel, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-s Allo one hydroxy stearic acid and the like esters such as cetearyl source. Examples of the hydrocarbon-based oils and fats include hydrocarbon-based oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline. Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, Alkyl modified silicone oil, amino modified silicone oil, etc. are mentioned. Perfluoro polyether etc. are mentioned as said fluorine-based fat or oil. Examples of the animal or vegetable oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, Cottonseed oil, Palm oil, Cuckoo nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba Animal or vegetable fats and oils, such as oil, candler wax, carnava wax, liquid lanolin, hardened castor oil, etc. are mentioned.

As said moisturizer, a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, a fat-soluble polymer, etc. are mentioned. Examples of the water-soluble low molecular moisturizer include serine, glutamine, sorbitol, mannitol, pyrrolidone sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned. Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters. Examples of the water-soluble polymers include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be mentioned. Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

As said emollient agent, long-chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, etc. are mentioned.

As said surfactant, a nonionic surfactant, anionic surfactant, cationic surfactant, an amphoteric surfactant, etc. are mentioned. Examples of the nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene), sorbitan fatty acid esters, and POE sorbitan fatty acid esters. , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric acid alkanolamide , Alkylamine oxide, hydrogenated soybean phospholipid, and the like. As the anionic surfactant, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkyl Amide phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfo acetate, acyl hydrolysis collagen peptide salt, perfluoroalkyl phosphate ester, etc. are mentioned. Can be. Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like. Examples of the amphoteric surfactant include a carboxybetaine type, an amidebetaine type, a sulfobetaine type, a hydroxysulfobetaine type, an amide sulfobetaine type, a phosphobetaine type, an aminocarboxylate type, an imidazoline derivative type, an amideamine type, and the like. Amphoteric surfactants; and the like.

The organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxide Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, coloramine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments, such as a silk powder, a cellulose, CI pigment yellow, and CI pigment orange, and the composite pigment of these inorganic pigments and organic pigments, etc. are mentioned.

As said organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl beta-alanine calcium, N-lauroyl beta-alanine zinc, and N-lauroyl glycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolysine, N-alpha-paratoylolnitine, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

As said ultraviolet absorber, paraamino benzoic acid, ethyl paraamino benzoate, amyl paraamino benzoate, octyl paraamino benzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid, and cinnamic acid Benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonic acid sodium salt, dihydroxybenzoic acid Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2- (2- And the like can be de-hydroxy-5-methylphenyl) benzotriazole.

Examples of the fungicides include hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, jinxylthione, benzalkonium chloride, Photosensitizer No. 301, sodium mononitro guicol, undecylenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium fmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Higher alcohols, such as cetyl alcohol, are mentioned as said alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight ratio with respect to a total weight, More preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the compound as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, emulsion, cream, foundation, lotion, essence, hair cosmetic, and the like. Specifically, the cosmetic composition of the present invention skin lotion, skin softener, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, hand cream, moisturizing cream, essence, pack, soap, lotion, milk lotion, gel, ointment, patch, Formulations of cleansing foam, body cleanser, astringent, spray. When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be. When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether. When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan. When the formulation of the present invention is a suspension, the carrier component is water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used. When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for explaining the present invention in more detail, the scope of the present invention is not limited thereto.

Example 1 Purification of Horse Oil

1. Extraction of dressing oil

4 kg of horseradish is pulverized with a blender, and 160g of activated carbon and 40g of diatomaceous earth are mixed with the pulverized malt to form a malt mixture, which is placed in a non-woven fabric and sealed with cotton cloth, and then placed in an extractor and water Additional 36 L was added to form a water mixture. The mixture of water in the extractor was heated at 90 ° C. for 1 hour, cooled to 72 ° C., filtered to obtain a filtrate by operating the extractor, and the filtrate was placed in a pail and allowed to stand, and then the bottom water layer was removed to obtain crude oil. This gave 2.5 liters of crude oil, with a residual weight of 1.3 kg in the nonwoven fabric.

2. Extraction of Refined Horse Oil

(1) Horse oil refined 1

2 parts by weight of dalsorb (dalsorb 2500M), 2 parts by weight of activated clay, and 6 parts by weight of activated carbon were mixed per 100 parts by weight of the crude oil to form a crude oil mixture, and the crude oil mixture was stirred at 80 ° C. for 60 minutes. After 40 minutes in the stirring process, 5 parts by weight of celite was additionally mixed. After 60 minutes of stirring, the crude horse oil mixture was filtered using a filter to obtain purified horse oil 1 (FIG. 2).

(2) Horse oil refined 2

Purified horse oil 2 was obtained in the same manner as in (1) of Example 1 2 except that 1 part by weight was used instead of 2 parts by weight of dalsorb.

(3) refined horse oil 3

Purified horse oil 3 was obtained in the same manner as in (1) of Example 1 2 except that 3 parts by weight were used instead of 2 parts by weight of dalsorb.

(4) Refined horse oil 4

Except not using celite, the other conditions were carried out similarly to (1) of Example 1, 2, and the refined horse oil 4 was obtained.

(5) Refined horse oil 5

The refined horse oil 5 was obtained in the same manner as in (1) of Example 1 except for the addition of 5 parts by weight of celite initially to form the crude oil mixture rather than 40 minutes later.

(6) Horse oil refined 6

Except for stirring at 100 degreeC instead of 80 degreeC, the other conditions were carried out similarly to (1) of Example 1, 2, and refined horse oil 6 was obtained.

(7) Refined horse oil 7

Purified horse oil 7 was obtained in the same manner as in Example 1 (2) except that 2 parts by weight of activated clay and 6 parts by weight of activated carbon were not used, and 8 parts by weight of activated clay was used.

(8) Refined horse oil 8

Except for using 2 parts by weight of activated clay and 6 parts by weight of activated charcoal and 8 parts by weight of activated carbon, other conditions were carried out in the same manner as in Example 1 (2) to obtain purified horse oil 8.

(9) Refined horse oil 9

Purified horse oil 9 was obtained in the same manner as in (1) of Example 1 except that 2 parts by weight of activated clay and 6 parts by weight of activated carbon were not used, and 4 parts by weight of activated clay and 4 parts by weight of activated carbon were used. .

(10) Refined Horse Oil 10

Refined horse oil 10 was obtained in the same manner as in (1) of Example 1 2 except that 0.2 parts by weight of BHT was further mixed to form a crude oil mixture.

(11) Refined Horse Oil 11

Purified horse oil 11 was obtained in the same manner as in (1) of Example 1 2 except that 0.5 parts by weight of BHT was further mixed to form a crude oil mixture.

Example 2 Measurement of Acid Value and Peroxide Value of Refined Horse Oil

1. The acid value and the peroxide value of the crude oil and refined horse oil obtained in Example 1 were measured, and the results at the time of manufacture are shown in Table 1, and the results are shown in Table 2 after storage at room temperature for 30 days. Acid values were determined according to a standard maintenance assay using 0.1 N KOH / Methanol solution, and peroxide values were determined according to AOAC range using saturated potassium iodide solution.

2. Compared with the results of refined horse oil 2 and 3 in Table 1 below, the peroxide value can not be lowered when dalsorb is used less, and the peroxide value decreases when the dalsorb is used a lot, but rather the acid value is lower. It can be seen that the increase. In addition, compared with the results of refined horse oil 4 and 5 and the result of refined horse oil 1, it can be seen that the peroxide value cannot be lowered when celite is not used or mixed at first, and the results of refined horse oil 8 and 9 In contrast with the results it can be seen that the peroxide value and the acid value can be lowered when activated clay and activated carbon are used in an appropriate range.

Peroxide value
(meq / kg) Acid
(mgKOH / g) Peroxide value
(meq / kg) Acid
(mgKOH / g) Dressage oil 20 2.0 Refined horse oil 6 0.38 0.35 Refined horse oil 1 0.4 0.33 Refined horse oil 7 1.4 0.43 Refined horse oil 2 2.8 0.22 Refined horse oil 8 1.2 0.40 Refined horse oil 3 0.42 0.66 Refined horse oil 9 0.8 0.37 Refined horse oil 4 2.6 0.15 Refined horse oil 10 0.6 0.26 Refined horse oil 5 2.2 0.35 Refined horse oil 11 1.2 0.30

3. From Table 2, it can be seen that the peroxide value can be kept low for a long time when BHT is further mixed, and the refined horse oil obtained according to the present invention can keep the acid value low regardless of the presence or absence of a preservative.

Peroxide value (0 days) Peroxide Value (30 Days) Acid value (0 days) Mountain price (30 days) Dressage oil 20 60 2.0 40 Refined horse oil 1 0.4 35 0.33 0.46 Refined horse oil 10 0.6 6 0.26 0.33 Refined horse oil 11 1.2 5 0.30 0.35

Example 3 Sensory Evaluation of Refined Horse Oil

1. Sensory evaluation was performed on the crude oil and refined horse oil obtained in Example 1, and the results are shown in Table 3 below. Sensory evaluation was conducted on odor and color. A 20-point scale was used to evaluate five points.

2. In Table 3, it can be seen that refined horse oil has a transparent color and does not smell compared to crude oil, and refined horse oil 6 has a yellowish color and received a low score in color. When forming the it can be seen that the functionality for the color is poor.

Dressage oil Refined horse oil 1 Refined horse oil 6 Refined horse oil 10 color 2 5 3 5 smell One 5 5 5

Example 4 Confirmation of Wrinkle Improvement Effect of Cosmetic Composition Using Refined Horse Oil

1. Preparation of Extract

After washing the fox leaf, Holly leaf, daffodil leaf, dung leaf, horsefly leaf, and horsefly leaf each with water, dried under natural light for 2 days, and then hot air dried at 60 ℃ for 14 hours, dried fox leaf, Holly leaf, daffodil leaf. Each of the leaves, horseradish leaves and horsetail leaves were added to 80% ethanol (5 L ethanol per 1 kg dried leaf each) was extracted for 24 hours at room temperature and the extract was removed from the filter paper (F1005, CHMLAB). Thereafter, the extract was concentrated under reduced pressure at 70 ° C. using a rotary vacuum concentrator (N-1110, Eyela, Japen) to completely remove ethanol, thereby extracting fox bead (sample 1), Holly (extract 2), and daffodil extract (sample). 3), Privet extract (Sample 4), horse valgus extract (Sample 5), Sample 1 and Sample 2 are mixed in a weight ratio of 1: 1 to prepare Sample 6, and Samples 1 and 3 are mixed in a 1: 1 weight ratio Sample 7 was prepared, and sample 8 was prepared by mixing samples 2, 4, and 5 in a weight ratio of 1: 1: 1.

2. Evaluation of Cytotoxicity by MTT Assay

Dispense the raw 264.7 cells equally into 5 × 10 ⁴ cells / well in a 96 well plate, incubate for 24 hours in a 37 ° C, 5% CO ₂ incubator, remove the culture solution, and sample 1 to 8 at each concentration. After diluting and adding, the cells were incubated at 37 ° C. and 5% CO ₂ incubator for 24 hours. EZ-Cytox (DoGenBio, Korea) was added 10% of the medium for each well and reacted for 3 hours at 37 ℃, 5% CO ₂ incubator and the absorbance at 540 nm with ELISA reader to determine the cell viability. As a result, it was confirmed that each of samples 1 to 8 did not show cytotoxicity as 100% cell viability at the concentration of 200 ug / mL or less.

3. Confirmation of wrinkle removal effect

(1) cell proliferation effect

Dispense HDFn cells equally into 96 well platedp 5.0x10 ⁴ cells / well, incubate for 24 hours in a 37 ° C, 5% CO ₂ incubator, remove cultures, replace with DMEM medium without FBS, and sample 1-8. Was added and incubated for 24 hours (final concentration 200 μg / mL for each of Samples 1-8). After incubation, 20 µl of MTT (3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium: 5mg / ml) solution is added to the reaction solution and then the reaction solution is removed for 10 hours. 200 μl of DMSO was added to each well to dissolve the stained cells, and the absorbance at 570 nm was measured. Cell proliferation was calculated and shown in Table 4 (cell proliferation (%) = (OD of sample-OD of control) / OD of control x 100). It can be seen that the samples 1 to 8 all improve the cell proliferation, and when using the sample 8 formed by mixing the samples 2, 4 and 5 in a weight ratio of 1: 1: 1, it can be seen that the cell proliferation can be further improved. .

Untreated Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Cell proliferation (%) 0 33 28 30 34 31 29 35 48

(2) collagen synthesis ability

Dispense HDFn cells equally into 96 well platedp 5.0x10 ⁴ cells / well, incubate for 24 hours in a 37 ° C, 5% CO ₂ incubator, remove cultures, and remove DMEM / F12 (3: 1) medium without FBS After replacement, samples 1-8 were added and incubated for 24 hours (final concentration 200 μg / mL for each of samples 1-8). Then, the procollagen amount was measured using the procollagen type 1 C-peptide (PIP) EIA kit, and the media supernatants were collected, and the results are shown in Table 5. Looking at Table 5, Samples 1 to 8 increase the amount of collagen 1 produced, and it can be seen that Sample 8 produces more collagen 1 than that of Samples 1 to 7.

Untreated Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 collagen 1 content (%) 100 132 129 137 133 135 129 136 149

(3) Sensory experiment on the wrinkle improvement effect

For women in their fifties without grade or chronic body disease, including skin diseases, the composition is divided into nine groups, and each of the compositions 1 to 9 is applied to the face every morning and evening for two weeks after cleansing. The effect was evaluated. As a result, in the case of composition 1, it was evaluated that there was no effect of wrinkle improvement, and in the case of compositions 2 to 9, it was evaluated that there was an effect of wrinkle improvement. Through this, it can be seen that Samples 1 to 8 improve the effect of wrinkle improvement. The composition 1 is composed of 7% by weight of glycerin, 1.5% by weight of stearic acid, 0.1% by weight of vitamin E, 1.4% by weight of horse oil (refined horse oil 1) and 90% by weight of water, each of the composition 2 to 9 each 5% by weight of water Instead of reducing the%, each of Samples 1 to 8 (5% by weight) of Example 4 was added to have the same composition as Composition 1 (ie, for Composition 2, Sample 1 (5% by weight), glycerin 7% %, 1.5% by weight stearic acid, 0.1% by weight vitamin E, horse oil (purified horse oil 1) 1.4% by weight and water 85% by weight).

In the above, the Applicant has described preferred embodiments of the present invention, but these embodiments are merely one embodiment for implementing the technical idea of the present invention, and any changes or modifications may be made as long as the technical idea of the present invention is implemented. Should be interpreted as being within the scope.

Claims (8)

It contains purified horse oil and extract for promoting collagen synthesis to provide skin wrinkle improvement effect,
The refined horse oil is a crude oil extraction step of extracting the crude oil from which impurities are removed by adding hot water to the raw material, and purifying the crude oil to adjust the acid value and peroxide value to obtain a colorless and odorless refined horse oil Manufactured through the oil phase
The dressing oil refining step is a crude dressing oil mixture by mixing 100 parts by weight of crude oil, 1.5 to 2.5 parts by weight of Dalsorb, 1.5 to 2.5 parts by weight of activated clay, 5 to 7 parts by weight of activated carbon, and 0.1 to 0.2 parts by weight of BHT. A first stirring step of forming and stirring the first crude oil mixture at 80 to 120 ° C. for 35 to 45 minutes, and further mixing 4 to 6 parts by weight of celite to the first crude oil mixture after the first stirring step A second stirring step of forming a second crude oil mixture and stirring the second crude oil mixture at 80 to 120 ℃ for 15 to 25 minutes, and filtering the second crude oil mixture after the second stirring step to obtain purified horse oil Including a obtaining step,
The extract for promoting the collagen synthesis to provide a skin wrinkle improvement effect is a cosmetic composition, characterized in that the horse's foot extract is used. delete delete The method of claim 1, wherein the dressing oil extraction step
A pulverizing step of pulverizing the dough to a certain size, a mixing step of mixing activated carbon and diatomaceous earth into the pulverized dough to form a dough mixture, and then wrapped the cloth dough mixture with a cloth and then immersed in water to mix the water mixture A hot water treatment step of forming and heating the water mixture, a filtration step of filtering the water mixture after the hot water treatment step to obtain a filtrate, and separating the filtrate obtained in the filtration step into an oil layer and a water layer and then separating the water layer. Cosmetic composition, characterized in that it comprises a separation step of obtaining a crude oil by removing the water. The method of claim 4, wherein
In the mixing step, activated carbon is used 3 to 5 parts by weight based on 100 parts by weight of the dough, diatomaceous earth is 0.5 to 2 parts by weight based on 100 parts by weight of the dough,
In the hot water treatment step, water is used 800 to 1000 parts by weight based on 100 parts by weight of the dough, the water mixture is heated for 50 to 70 minutes at a temperature of 85 to 95 ℃,
The filtration step is a cosmetic composition, characterized in that is carried out after cooling the water mixture to 68 to 70 ℃. delete delete delete

Images