KR102350003B1 - Cosmetic composition for skin whitening - Google Patents

Abstract

The present invention relates to a cosmetic composition that can provide a skin whitening effect, including lava seawater, an oleifera extract and hydrogen.

Description

Cosmetic composition for skin whitening

The present invention relates to a cosmetic composition capable of providing an improved skin whitening effect, including lava seawater, a dolme leaf extract, and hydrogen.

Cosmetics are articles used on the human body to clean and beautify the human body to add attractiveness, to brighten the appearance, or to maintain or promote skin and hair health, meaning that it has a slight effect on the human body. Recently, by improving the function of conventional cosmetics, functional cosmetics that help whiten the skin, help improve wrinkles on the skin, burn the skin finely, or help protect the skin from UV rays are in the spotlight. The following patent documents show an example of functional cosmetics.

<Patent Literature>

Patent 1: No. 10-1550422 (registration on Aug. 31, 2015) "Cosmetic composition containing indigo plant extract"

Patent 2: No. 10-1569431 (registered on November 10, 2015) "Method for improving skin care in 6 steps using a cosmetic composition containing Illite extract, which has excellent skin whitening and wrinkle improvement effects"

However, conventional cosmetic compositions and methods of applying them have problems in that the skin improvement effect is not satisfactory and stability is not guaranteed.

The present invention is to solve the problems of the prior art described above,

It is an object of the present invention to provide a cosmetic composition having an improved skin whitening effect, including lava seawater, an oleifera extract and hydrogen.

In addition, an object of the present invention is to provide a cosmetic composition having an additional wrinkle-improving effect by further including an extract for wrinkle-improving.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the cosmetic composition according to the present invention is characterized in that it contains lava seawater, a dolme leaf extract, and hydrogen.

According to another embodiment of the present invention, the cosmetic composition according to the present invention is characterized in that the cosmetic composition provides a skin whitening effect.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the lava seawater has a hardness of 4000 to 6000, and 0.1 to 10 parts by weight of the lava seawater extract is used based on 100 parts by weight of the lava seawater, and the hydrogen is It is characterized in that it is directly dissolved in lava seawater and has a dissolved concentration of 0.5 to 1.5 ppm.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further comprises an extract for wrinkle improvement, and the wrinkle-improving extract includes a daffodil extract, a champignon extract, a horseradish extract, and a fenugreek extract characterized in that

The present invention can obtain the following effects by the present embodiment above.

The present invention has the effect of providing an improved skin whitening effect, including lava seawater, stony leaf extract and hydrogen.

In addition, the present invention has an effect that can provide an additional wrinkle improvement effect by additionally comprising an extract for wrinkle improvement.

1 is a photograph of a cell culture medium for confirming the whitening effect of a cosmetic composition according to an embodiment of the present invention.
Figure 2 is a photo of cell sediment for confirming the whitening effect of the cosmetic composition according to an embodiment of the present invention.

Hereinafter, the cosmetic composition for skin whitening according to the present invention will be described in detail with reference to the accompanying drawings. Unless otherwise defined, all terms in this specification have the same general meaning as understood by those of ordinary skill in the art to which the present invention belongs, and in case of conflict with the meaning of the terms used in this specification, the According to the definition used in the specification. In addition, detailed descriptions of well-known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

The cosmetic composition according to an embodiment of the present invention contains lava seawater, dolex leaf extract and hydrogen, and provides a skin whitening effect. The lava seawater refers to water that is naturally filtered by the volcanic rock layer and seeps into the ground underground, and lava seawater having a hardness of 4000 to 6000 is preferably used. The doloe is a dicotyledonous plant, a perennial tendril plant of the Gourdaceae family, and the doloe leaf extract is dried and pulverized, then 10 times the weight of ethanol is added, stirred, extracted for 48 hours, filtered, concentrated under reduced pressure, and freeze-dried. It is formed, and the extract is preferably used in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of lava seawater. The hydrogen is dissolved by directly injecting into water, and it is preferable that the dissolved concentration is 0.5 to 1.5 ppm. The cosmetic composition is mixed with lava seawater of a certain hardness, extract and hydrogen in a certain ratio to provide a more improved skin whitening effect.

The cosmetic composition according to another embodiment of the present invention may further include an extract for improving skin wrinkles. The extract for improving skin wrinkles may include any one or more extracts selected from the group consisting of daffodils extract, pine needle extract, horsetail extract, and fenugreek extract. The narcissus is a monocotyledonous plant Liliaceae Narcissus, the cypress is a dicotyledonous plant, a perennial herb of the Hyonsaceae family, and the horsetail is a dicotyledonous plant, a deciduous shrub of the Rosaceae family, and the perennial herbaceous plant is an evergreen perennial creeper. to be. The daffodil extract, cypress extract, horseradish extract and perennials extract are dried and pulverized, respectively, of daffodil, chrysanthemum, horsetail, and perennials, followed by addition of an extraction solvent, filtration, concentration under reduced pressure, and freeze-drying It can be obtained through the process.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

As the water-soluble vitamin, any one can be used as long as it can be formulated in cosmetics. Preferably, vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. and their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) are also water-soluble vitamins that can be used in the present invention. included in The water-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method or a chemical synthesis method.

The oil-soluble vitamins may be any as long as they can be formulated in cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol). and their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. The oil-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a purification method from a microbial culture, an enzyme or a chemical synthesis method, and the like.

The high molecular weight peptide may be any compound as long as it can be incorporated into a cosmetic. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are mentioned. The polymer peptide can be purified and obtained by a conventional method such as purification from a microbial culture solution, an enzyme method, or a chemical synthesis method, or can be purified from natural products such as the dermis of pigs or cattle, silk fibers of silkworms, etc. .

The high molecular weight polysaccharide may be any compound as long as it can be formulated in cosmetics, but preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (such as sodium salt). For example, chondroitin sulfate or a salt thereof can be purified and used from mammals or fish.

The sphingolipid may be any as long as it can be blended into a cosmetic, and preferred examples include ceramide, phytosphingosine, and sphingoglycolipid. The sphingo lipid may be purified by a conventional method from mammals, fish, shellfish, yeast or plants, or may be obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be formulated in cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc., and calrageenan purified from these seaweed extracts, arginic acid, sodium alginate , potassium alginate, etc. are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from seaweed by a conventional method.

The cosmetic composition of the present invention may contain other ingredients that are usually formulated in the cosmetic composition. For example, oils and fats, moisturizing agents, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, A cooling agent, an antiperspirant agent, purified water, etc. are mentioned.

Examples of the fats and oils include ester fats and oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats, and vegetable oils. Examples of the ester-based fats and oils include glyceryl tri-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearate. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adi Diisopropyl finate, isoalkyl neopentanoate, tri(caprylic acid, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaellisly tetra2-ethylhexanoate Tall, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isos laurate Tearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, 2-ethylhexanoate ceto Stearyl, 2-ethyl hexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic acid, capric acid) propylene glycol, dicaprylic acid propylene glycol, Neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecyl acid, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostea octanoate Reel, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerol isostearate ester , triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, mal Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, diox sebacate tyl, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stearate Ester systems, such as isocetyl alloyl hydroxy stearate, 12-stealoyl hydroxy stearate stearate, and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned. Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum. Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, Alkyl-modified silicone oil, amino-modified silicone oil, etc. are mentioned. As said fluorine-type fats and oils, perfluoro polyether etc. are mentioned. Examples of the animal or vegetable oils and fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, saffron oil, soybean oil, corn oil, rapeseed oil, passerine oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, cranberry colostrum, marcus damia nut oil, meadow home oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rafi oil, jojoba and animal or plant oils and fats such as oil, candelabra wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low molecular weight moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. As the water-soluble low molecular weight moisturizer, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n=2 or more), polyglycerol B (polymerization degree n=2 or more), lactic acid, lactic acid salt, etc. are mentioned. Cholesterol, cholesterol ester, etc. are mentioned as said fat-soluble low molecular weight moisturizing agent. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, and the like. can be heard Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicocene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular weight silicone.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, etc. are mentioned as said surfactant. Examples of the nonionic surfactant include self-emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, POE sorbitan fatty acid ester , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether-modified silicone, lauric acid alkanolamide , alkylamine oxide, hydrogenated soybean phospholipid, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, and alkyl. Amide phosphate, alkyloyl alkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. can Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salt of lanolin derivative, etc. are mentioned. Examples of the amphoteric surfactant include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. of amphoteric surfactants, and the like.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, oxide inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, and organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, a divinylbenzene-styrene copolymer, ethylene tetrafluoride, etc. are mentioned.

Examples of the ultraviolet absorber include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, cinnamic acid. Benzyl, para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexaneglyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture; Urocanic acid, ethyl urokanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

Examples of the disinfectant include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, Photosensitizer No. 301, mononitroguaranteol sodium, undecyrenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and ellisorbic acid.

Citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate etc. are mentioned as said pH adjuster.

As said alcohol, higher alcohols, such as cetyl alcohol, are mentioned.

In addition, blending components that may be added other than this are not limited thereto, and any of the above components can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5% by weight relative to the total weight, More preferably, it is blended in a weight percentage of 0.01 to 3%.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the compound as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances. and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, emulsion, cream, foundation, lotion, cosmetic liquid, hair cosmetics, and the like. Specifically, the cosmetic composition of the present invention is a skin lotion, skin softener, moisture lotion, nourishing lotion, massage cream, nourishing cream, hand cream, moisture cream, essence, pack, soap, lotion, milk lotion, gel, ointment, patch, Includes cleansing foam, body cleanser, astringent, and spray formulations. When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether. When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan. When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used. When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of samples

1. Preparation of samples for whitening

(1) After drying and pulverizing the stone leaves, 10 times the weight of ethanol was added thereto, extracted for 48 hours with stirring, filtered, concentrated under reduced pressure, and freeze-dried to obtain an extract of the stone outer leaves (a sample of stone leaves).

(2) Distilled water, lava seawater, dolme leaf extract, hydrogen, etc. were mixed in the ratio shown in Table 1 below to obtain whitening samples 1 to 14. The lava seawater was extracted and treated in Gujwa-eup, Jeju-si, and the hardness was adjusted.

Distilled water (parts by weight) Lava seawater (parts by weight) Stone sample (parts by weight) Hydrogen
(Dissolved concentration (ppm)) whitening sample 1 100 0 0 0 whitening sample 2 100 0 2 0 whitening sample 3 100 0 0 One whitening sample 4 100 0 2 One whitening sample 5 0 100 (hardness 5000) 0 0 whitening sample 6 0 100 (hardness 5000) 2 0 whitening sample 7 0 100 (hardness 5000) 0 One whitening sample 8 0 100 (hardness 5000) 2 One whitening sample 9 0 100 (hardness 3000) 2 One whitening sample 10 0 100 (hardness 7000) 2 One whitening sample 11 0 100 (hardness 5000) One One whitening sample 12 0 100 (hardness 5000) 3 One whitening sample 13 0 100 (hardness 5000) 2 0.7 whitening sample 14 0 100 (hardness 5000) 2 1.3

2. Preparation of samples for wrinkle improvement

Daffodil extract by adding 10 times the weight of ethanol to each of the dried and pulverized daffodils (root), cypress (leaf), horsetail (leaf), and perennial herb (leaf), stirred for 48 hours, filtered, concentrated under reduced pressure, and freeze-dried (wrinkle sample 1), cypress extract (wrinkle sample 2), horsetail extract (wrinkle sample 3) and fenugreek extract (wrinkle sample 4) were prepared.

<Example 2> Confirmation of whitening effect

1. Confirmation of whitening effect by measuring melanin production

(1) To measure melanin production in B16-F10 melanoma cells, B16-F10 melanoma cells were treated for 72 hours using each of whitening samples 1 to 14 as a culture medium, and 200 nM of α-MSH was added during the treatment. did Thereafter, the cells were lysed using 1M NaOH containing 10% DMSO, and the melanin content was measured at 409 nm wavelength using a spectrophotometer, and corrected by the amount of protein, and the results are shown in Table 2 below.

(2) Referring to Table 2, it can be seen that lava seawater, dolex leaf extract, and hydrogen each have the effect of inhibiting melanin production, and more melanin is produced when lava seawater of a certain hardness, extract and hydrogen are mixed in a certain ratio. It can be seen that suppress

Melanin content (% of control)-α-MSH untreated whitening sample
One whitening sample
2 whitening sample
3 whitening sample
4 whitening sample
5 whitening sample
6 whitening sample
7 100 93 94 82 92 82 83 whitening sample
8 whitening sample
9 whitening sample
10 whitening sample
11 whitening sample
12 whitening sample
13 whitening sample
14 68 75 74 75 73 76 78 Melanin content (% of control)-α-MSH treatment whitening sample 1 whitening sample 2 whitening sample 3 whitening sample 4 whitening sample 5 whitening sample 6 whitening sample 7 225 205 202 190 200 170 160 whitening sample 8 whitening sample 9 whitening sample 10 whitening sample 11 whitening sample 12 whitening sample 13 whitening sample 14 125 156 160 151 154 152 150

2. Confirmation of whitening effect through visual confirmation of cell culture medium and cell sediment

(1) After treating B16-F10 melanoma cells in the same manner as in Example 2 by varying the content of the whitening sample 8, the cell culture medium was photographed and shown in FIG. 1, and the cell sediment was photographed in FIG. indicated. 1 and 2, (a) is when whitening sample 8 and α-MSH are not used, (b) is when whitening sample 8 is not used and α-MSH is used, (c) is whitening sample 8 When 25% of the culture medium is used and α-MSH is used, in (d) when 50% of the culture medium is used for whitening sample 8 and α-MSH is used, (e) when whitening sample 8 is used with 100% of the culture medium The case where α-MSH is used is shown.

(2) Referring to FIGS. 1 and 2 , it can be seen that the more the amount of the composition (whitening sample 8) mixed with lava seawater of a specific hardness, the stony leaf extract and hydrogen in a specific ratio, the more the pigment decreases, so the cosmetic composition of the present invention It can be seen that can provide a more improved whitening effect.

<Example 3> Confirmation of wrinkle improvement effect

1. Confirmation of wrinkle improvement effect through cell proliferation

(1) In order to measure the proliferative effect of human fibroblasts, human fibroblasts were treated for 48 hours in DMEM culture medium to which wrinkle samples 1 to 4 of the same concentration were added respectively (final concentration of each of wrinkle samples 1 to 4 100 μg) /mL), MTT (3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyl tetrazolium) solution was added, and after 6 hours of reaction, the reaction solution was removed. After dissolving the stained cells by adding DMSO to each well, absorbance was measured at 570 nm using a spectrophotometer, and the cell proliferation rate (%) was calculated and shown in Table 3. of absorbance - absorbance of control group) / absorbance of control group × 100).

(2) Referring to Table 3, it can be seen that all of the wrinkle samples 1 to 4 proliferate cells, thereby improving skin wrinkles.

unprocessed Wrinkle sample 1 Wrinkle sample 2 Wrinkle sample 3 Wrinkle sample 4 Cell proliferation (%) 0 25 27 31 23

2. Confirmation of wrinkle improvement effect through collagen production

(1) In order to measure the collagen production effect in human fibroblasts, human fibroblasts were treated for 48 hours in DMEM culture medium to which wrinkle samples 1 to 4 of the same concentration were added, respectively (final concentration of each of wrinkle samples 1 to 4 was 100 μg/mL), the supernatant of the culture medium was collected, and the amount of procollagen was measured using the Procollagen type 1 C-peptide EIA kit, and the results are shown in Table 4.

(2) Referring to Table 4, it can be seen that all wrinkle samples 1 to 4 increase collagen synthesis, thereby improving skin wrinkles.

unprocessed Wrinkle sample 1 Wrinkle sample 2 Wrinkle sample 3 Wrinkle sample 4 Cell proliferation (%) 0 131 135 127 121

<Example 4> Confirmation of skin irritation, whitening and wrinkle improvement through skin patch test

1. A cosmetic composition sample was prepared in the composition ratio of Table 5 below.

cosmetic composition sample content(%) whitening sample 8 wrinkle sample antiseptic glycerin Purified water One 50 0 0.05 2 remaining amount 2 50 1 (wrinkle sample 1) 0.05 2 remaining amount 3 50 1 (wrinkle sample 2) 0.05 2 remaining amount 4 50 1 (wrinkle sample 3) 0.05 2 remaining amount 5 50 1 (wrinkle sample 4) 0.05 2 remaining amount 6 0 1 (wrinkle sample 1) 0.05 2 remaining amount

2. 26 women in their 40s without skin disease were divided into 6 groups, and after washing their face every morning and evening for 6 weeks, each of the cosmetic composition samples 1 to 6 were used (after soaking the cosmetic composition sample on a sheet, the above It proceeds by attaching the sheet to the skin for a certain period of time). Thereafter, the sensory evaluation for skin improvement was performed and the wrinkle reduction rate was calculated, and it is shown in Table 6. In the sensory evaluation, the occurrence of skin irritation, the degree of improvement in skin color, and the degree of improvement in wrinkles were checked. In the items of improvement in skin color and wrinkle, if the condition was good, it was evaluated as 5 points and if the condition was bad, it was evaluated as 0 points, and the wrinkle reduction rate was fine. Measuring around the eye area using a measuring instrument, comparing the average depth of wrinkles before and after using the cosmetic composition sample to calculate the reduction rate (%), based on the cosmetic composition sample 1, the relative reduction rate (%) was calculated. .

3. Referring to Table 6, it can be seen that all of the cosmetic composition samples 1 to 6 do not cause skin irritation, the skin color is brightened when the whitening sample 8 is used, and skin wrinkles are reduced when the wrinkle samples 1 to 4 are used. can be seen to decrease.

cosmetic composition sample One 2 3 4 5 6 Whether skin irritation × × × × × × skin color improvement 4.3 4.2 4.4 4.5 4.1 3 wrinkle improvement 3.4 4.1 4.2 4.3 4.0 3.8 Wrinkle reduction rate (%) 0 17 20 19 16 12

In the above, the applicant has described preferred embodiments of the present invention, but these embodiments are only one embodiment that implements the technical idea of the present invention, and any changes or modifications as long as the technical idea of the present invention is implemented. should be construed as within the scope.

Claims (4)

In the cosmetic composition,
The cosmetic composition contains lava seawater, dolme leaf extract and hydrogen,
The cosmetic composition further comprises an extract for wrinkle improvement,
The extract for wrinkle improvement is a cosmetic composition, characterized in that Malvaldori extract or fenugreek extract is used. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that providing a skin whitening effect. 3. The method of claim 2,
The lava seawater has a hardness of 4000 to 6000, and 0.1 to 10 parts by weight is used based on 100 parts by weight of the lava seawater, and the hydrogen is dissolved directly in the lava seawater so that the dissolved concentration is 0.5 to 1.5 ppm. a cosmetic composition. delete

Images