KR102121302B1 - Cosmetic composition using Cinnamomum camphora extract - Google Patents

Abstract

The present invention relates to a cosmetic composition having an anti-inflammatory, antibacterial, and exfoliating effect by containing camphor extract.

Description

Cosmetic composition using camphor extract {Cosmetic composition using Cinnamomum camphora extract}

The present invention relates to a cosmetic composition having an anti-inflammatory, antibacterial, and exfoliating effect by containing camphor extract.

Cosmetics are products used in the human body to clean and beautify the human body to add attractiveness, change the appearance brightly, or maintain or promote the health of the skin and hair. As the economy develops and the standard of living increases, as in the following patent literature Functional cosmetics have been widely developed to provide skin inflammation relief, antioxidant, whitening, and wrinkle improvement effects.

<Patent Document>

Patent Publication No. 10-1077824 (announced on Oct. 28, 2011) "Cosmetic composition for improving atopy containing illite extract and method for manufacturing the same"

However, the conventional cosmetic composition has a problem in that the effect of alleviating inflammation is not satisfactory, and stability is not guaranteed by using minerals or the like.

The present invention is to solve the problems of the prior art described above,

An object of the present invention is to provide a cosmetic composition capable of providing various useful components of camphor tree extract.

In addition, the present invention has a purpose to provide a cosmetic composition capable of exerting a new effect that the camphor extract does not have by using a specific extraction method or by using an additional component.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the cosmetic composition according to the present invention includes a camphor extract, and the camphor extract is mixed with camphor leaves in ethanol, after a certain period of time, the dried extract is removed and the obtained extract is concentrated under reduced pressure and lyophilized. And anti-inflammatory, antibacterial and exfoliating effects.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further comprises a hwangchil tree extract, the camphor tree extract and hwangchil tree extract are mixed in a weight ratio of 1:1, the hwangchil tree extract is from hwangchil tree The mixing and stirring step of forming the mixed solution by mixing the obtained hwangchil with an extraction solvent, and a separation step of separating the layer into parts that are dissolved in the extraction solvent and parts that are not dissolved by standing at a constant temperature for a period of time after the mixing stirring step In the separation step, the supernatant, which is the portion soluble in the extraction solvent, is separated and evaporated with the extraction solvent using a vacuum rotary evaporator to be obtained through an evaporation step of extracting the hwangchil tree extract. It is characterized by ultrasonic treatment and stirring.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further includes an extract for improving wrinkles, and the extract for improving wrinkles is Gin Giananam extract, Persimmon tree extract, Dampalsu extract, Fusicum extract And it characterized in that it further comprises any one or more selected from the group consisting of oak tree extract.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further includes an extract for improving wrinkles, and the extract comprises a Ginia male extract, a persimmon tree extract and an oak tree extract, The male extract, persimmon tree extract and oak tree extract are characterized by being mixed in a weight ratio of 1:1:1.

The present invention can obtain the following effects by the present embodiment.

The present invention has an effect that can provide a variety of useful components that camphor extract has.

In addition, the present invention has an effect of exerting a new effect that camphor extract does not have by using an additional component.

Hereinafter, a cosmetic composition comprising a camphor tree extract according to the present invention will be described in detail. Unless otherwise specified, all terms in this specification are the same as the general meaning of the term understood by a person skilled in the art to which the present invention belongs, and if there is a conflict with the meaning of the term used in the present specification, It follows the definition used in the specification. In addition, detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention are omitted. Throughout the specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

The cosmetic composition according to an embodiment of the present invention includes camphor extract. The camphor tree extract mainly provides anti-inflammatory, antibacterial, and exfoliation effects. The cosmetic composition will be used in the manufacture of cosmetics for various purposes such as bathing agents. The camphor tree extract is obtained by mixing the camphor tree leaves with ethanol, and after a certain period of time, the dry matter is removed and the obtained extract is concentrated under reduced pressure and freeze-dried. The camphor tree is an evergreen broad-leaved tree about 20m tall, the young branches are yellow-green, the leaves are misaligned, glossy, and the flowers bloom in bunches on the newly released branches of the armpits in May, turn white, and turn yellow, and 12 stamens It is arranged in 4 rows, and the anther is in the center of the operating table.

The cosmetic composition may further include a hwangchil tree extract. The camphor tree extract and hwangchil tree extract are preferably mixed in a weight ratio of 1:1. When the hwangchil tree extract is additionally used in the cosmetic composition, the anti-inflammatory effect may be further improved. The hwangchil tree is an evergreen tree of the dicotyledonous mountain tree, elm tree, reaching 15m in height, the young branches are green and hairless, the leaves are misaligned, egg-shaped or elliptical, the leaf edges are flat, but split into 3 to 5 in the young tree and sawtooth. Flowers bloom in light yellow-green in June to mid-August, male and female, run on mountain inflorescence, stalk is 3~5cm long, stalk is 5~10mm long, calyx is divided into 5, petals Fruits are 5 each, and there is a honey gland in the flower bed, pistils are divided into 5, and the nucleus is oval, and the fruit is opened in black from late September to November. In the hwangchil tree, hwangchil can be collected, and the hwangchil can be obtained directly from a hwangchil tree or a commercially available product, and commercialized hwangchil powder may be used.

The hwangchil tree extract mixes the hwangchil obtained from the hwangchil tree with an extraction solvent to form a mixed solution and stirs the mixture, and the mixture is dissolved in an extraction solvent by standing at a constant temperature for a period of time after the mixing and stirring. It is obtained through an evaporation step of separating a layer into parts and evaporating the extraction solvent by using a rotary evaporator by separating the supernatant, which is a part dissolved in the extraction solvent, in the separation step. For the extraction solvent, for example, ethanol, purified water, etc. may be used, and in the separation step, the upper layer liquid in which the hwangchil is dissolved in the extraction solvent, and the lower precipitate layer in the form of a powder in which the hwangchil is insoluble in the extraction solvent. Will be separated. When using the hwangchil itself, the color of the prepared cosmetic composition changes to yellow over time, thereby harming the aesthetics of the cosmetic composition and having a high viscosity, while the cosmetic composition is not a hwangchil but a hwangchil tree extract is used, The viscosity is lowered to improve the processability, and even after a preparation of the cosmetic composition, the color does not change to yellow and the transparency increases, so that the appearance is not impaired. The hwangchil tree extract can improve the extraction efficiency of the hwangchil tree extract when ultrasonically treating and stirring the mixed solution in the mixing and stirring step, which is one step in the process of obtaining. The ultrasonic treatment may be performed using an ultrasonic disperser.

The cosmetic composition may further include an extract for improving skin wrinkles. Extracts for improving the skin wrinkles can be used any one or more selected from the group consisting of gingianam extract, persimmon tree extract, dampalsu extract, papyrus extract and oak tree extract, gingianam extract, persimmon tree extract and It is more preferable that the oak tree extract is used in a weight ratio of 1:1:1. The extract for improving wrinkles will be contained in an amount of 0.001 to 10% based on the total weight of the cosmetic composition. The Gin Giananam extract, persimmon tree extract, Dampalsu extract, Hui Feng tree extract and Oak tree extract are mixed with each of Gin Gianam leaves, Persimmon tree leaves, Dampal water leaves, Hui Fang tree leaves, and Oak tree leaves in ethanol, and after a certain time The extract was removed and the obtained extract was concentrated under reduced pressure and freeze-dried. The Gin Gianam is an evergreen perennial plant of the Orchidaceae family, which has not large flowers and has nodes on the stem like bamboo, blooms in early spring, stops growing in summer, and has pink and white flowers. The persimmon tree is an evergreen small tree belonging to the persimmon tree family. The leaves are alternate, thick, oval or similar, and the flowers bloom in March and April. The Dampalsu is an evergreen tree belonging to the Dampalaceae family, reaching 20m in height, without hair, the leaves are coarse, lanceolate, thick, with shallow serrations on the edges, the flowers bloom in July, and are arranged as gunshots, the fruits are nuclei, ellipsoids. It is 2~2.5cm long and ripens in black purple in September. The barkwood is an evergreen tree of the dicotyledonous sapling tea tree family, and the leaves run alternately, but they gather together at the ends of the branches and run upside down, and they are elongated oval shaped like an upside-down shape, or upside-down, and the edges of the leaves are flat, thick and shiny. There is and the petiole is red. The oak tree is an evergreen tree of the dicotyledon family Myrtlewood, camphoraceae, grows on a sandy beach, reaches 10m in height and has small branches green, leaves are thick, long oval, flat at the edges, and have three distinct veins. Green and white on the back.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any compound that can be blended into a cosmetic, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. Water-soluble vitamins that can be used in the present invention, such as salts (thiamine hydrochloride, sodium ascorbate salt) or derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt) Is included in. The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of microorganisms, an enzyme method or a chemical synthesis method.

The oil-soluble vitamin may be any compound that can be blended into a cosmetic, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And their derivatives (ascorbyl palmitate, ascorbyl stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol acetate nicotine E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl Ethyl ether, etc.) are also included in the useful vitamin used in the present invention. The oil-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of microorganisms, an enzyme or a chemical synthesis method.

The polymer peptide may be any compound as long as it can be blended into a cosmetic, preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. The polymer peptide may be purified by a conventional method such as a purification method from a culture medium of microorganisms, an enzyme method, or a chemical synthesis method, or may be purified from natural substances such as dermis such as pigs and cows, silkworm silkworms, and the like. .

The polymer polysaccharide may be any compound as long as it can be blended into a cosmetic. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate, or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be any compound as long as it can be blended into a cosmetic, and preferably, ceramide, phytosphingosine, sphingoglycolipid, and the like. The sphingoli lipid can be purified by a conventional method from a mammal, fish, shellfish, yeast or plant, or can be obtained by chemical synthesis.

The seaweed extract may be anything as long as it can be blended into a cosmetic, but preferably, brown seaweed extract, red seaweed extract, green algae extract, etc., and further, calagazine, arginic acid, and sodium arginate purified from these seaweed extracts , Potassium arginate and the like are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from seaweed by a conventional method.

The cosmetic composition of the present invention can be blended with other ingredients that are usually blended into the cosmetic composition. For example, fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavors, blood circulation promoters, And cooling agents, limiting agents, and purified water.

Examples of the fat or oil component include ester fats and fats, hydrocarbon fats and fats, silicone fats and fats, fluorine fats and fats, animal fats, and vegetable fats and oils. Examples of the ester oils and fats include tri2-ethylhexanoate glyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diadi Diisopropyl finate, isoalkyl neopentanoate, tri(capryl, capric acid)glyceryl, trimethylethylpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelisley tritetraethylethylhexanoate Tol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl riinooleate, and lauric acid source Tearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, isopropyl isostearate, 2-ethylhexanocetoceto Stearyl, 2-ethylhexanoate stearyl, isostearate hexyl, dioctanoate ethylene glycol, dioleate ethylene glycol, dicapric propylene glycol, di(capryl, capric acid) propylene glycol, dicaprylic acid propylene glycol, Neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearate octanoate Reel, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerin isostearic acid ester , Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, mal Diisostearyl acid, 2-ethylhexyl hydroxystearic acid, 2-ethylhexyl disuccinate, diisobutyl adipic acid, diisopropyl sebacate, dioxe sebacate Tyl, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phyteryl oleate, 12-stearate And ester systems such as isocetyl alloylhydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate. Examples of the hydrocarbon-based oil and fat include hydrocarbon-based oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and waselin. Examples of the silicone oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane·methylcetyloxysiloxane copolymer, dimethylsiloxane·methylstearoxylsiloxane copolymer, And alkyl-modified silicone oils, amino-modified silicone oils, and the like. Examples of the fluorine-based fats and oils include perfluoropolyethers. Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes Seed Oil, Cottonseed Oil, Palm Oil, Cucumin Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Moon Seed Colostrum, Marker Demi Nut Oil, Meadow Home Oil, Yolk Yolk Oil, Uji, Horse Oil, Mink Oil, Orange Rape Oil, Jojoba And animal or plant fats and oils such as milk, candelary wax, carnauba wax, liquid lanolin, and hardened castor oil.

Examples of the moisturizing agent include water-soluble low molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and fat-soluble polymers. Examples of the water-soluble low molecular humectant include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly And propylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid, and the like. Examples of the fat-soluble low-molecular moisturizer include cholesterol and cholesterol esters. Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. Can be lifted. Examples of the fat-soluble polymer include polyvinylpyrrolidone-eicosen copolymer, polyvinylpyrrolidone-hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants. Examples of the nonionic surfactant include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, and POE sorbitic fatty acid ester. , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric acid alkanolamide , Alkylamine oxide, hydrogenated soybean phospholipid, and the like. Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl Amide phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Can be. Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, And benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropylamide stearate, and quaternary ammonium salts of lanolin derivatives. Examples of the amphoteric surfactant include carboxybeta type, amide betaine type, sulfobetaine type, hydroxy sulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. And amphoteric surfactants.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxidation. Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzeneㆍstyrene copolymer, And organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and composite pigments of inorganic pigments and organic pigments.

As said organic powder, Metal soap, such as calcium stearate; Alkyl phosphate metal salts such as sodium cetyl acid zinc, lauryl acid zinc, and calcium lauryl acid; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylazine, N-alpha-paritoylolnitin, N-alpha-lauroylarginine, N-alpha-cured Uji fatty acid acyl arginine -Acyl basic amino acids; N-acylpolypeptides, such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-amino caprylic acid and alpha-amino lauric acid; And polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, and ethylene tetrafluoride.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, cinnamic acid Benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl-diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- And 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, and the like.

Examples of the fungicide include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincfilionone, benzalkonium chloride, Photoresist 301, mononitrowire sodium, undeciric acid, and the like.

Examples of the antioxidants include butyl hydroxyanisole, propyl gallic acid, and elisoric acid.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, phthalic acid, sodium phthalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component that may be added in addition is not limited to this, and any of the above components can be blended within a range not impairing the objects and effects of the present invention, but preferably from 0.01 to 5% by weight, based on the total weight, More preferably, it is blended at 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of solutions, emulsions, viscous mixtures, and the like.

The components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the above-mentioned compounds as active ingredients, and for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments, and fragrances. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, emulsion, cream, foundation, lotion, cosmetic liquid, hair cosmetic, and the like. Specifically, the cosmetic composition of the present invention is a skin lotion, skin softener, moisture lotion, nutrition lotion, massage cream, nutrition cream, hand cream, moisture cream, essence, pack, soap, lotion, milk lotion, gel, ointment, patch, Contains formulations of cleansing foam, body cleanser, astringent, and spray. When the formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. Can be. When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether. When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester. When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used. When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide as a carrier component Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of sample

(1) Camphor tree extract sample

250 g of dried and crushed camphor tree (leaf) was immersed in 500 ml of ethanol for 36 hours to extract and evaporate the extract under reduced pressure to obtain a camphor extract (sample 1).

(2) Hwangchil tree extract sample

1) Commercially available hwangchil powder is mixed with ethanol as an extraction solvent (500 ml of ethanol per 100 g of hwangchil powder), stirred for 2 hours, and left at room temperature for 36 hours to dissolve in the supernatant and the extraction solvent, which are the parts dissolved in the extraction solvent. The layer separation was performed with the precipitate layer, which was not part, and the extraction solvent was evaporated from the separated supernatant using a vacuum evaporator to obtain a hwangchil tree extract (sample 2).

2) In Example 1 (2) 1), except for mixing the hwangchil powder and the extraction solvent and ultrasonicating for 20 minutes using an ultrasonic disperser and then stirring, other conditions were applied to Example 1 (2) 1 ) To obtain hwangchil tree extract (sample 3).

3) The Hwangchil powder used in Example 1 (2) 1) was prepared as Sample 4.

(3) Extract sample for wrinkle improvement

250 g each of dried and crushed gingi anam (leaves), persimmon tree (leaves), dampalsu (leaves), barkwood (leaves), and oak trees (leaves) are immersed in 500 ml of ethanol for 36 hours to extract and evaporate the extract under reduced pressure. , Gin Gianam extract (Sample 5), Persimmon tree extract (Sample 6), Dampalsu extract (Sample 7), Fusicum extract (Sample 8), Oak tree extract (Sample 9), Samples 5 and 7 in 1:1 Prepare sample 10 by mixing in a weight ratio, prepare samples 11 by mixing samples 5 and 9 in a 1:1 weight ratio, and prepare sample 12 by mixing samples 5, 6, and 9 in a weight ratio of 1:1:1. Did.

<Example 2> Cytotoxicity evaluation

MTT assay was performed to examine the toxicity of samples 1 to 3 and 5 to 12. The cultured RAW 264.7 cells were treated with LPS (1 μg/mL) and samples 1 to 3 and 5 to 12, respectively, and cultured for 24 hours. After the culture was completed, 10 μL of MTT solution was added and cultured for 8 hours. After removing the supernatant, put DMSO, shake well using a plate mixer, and measure absorbance at 540 nm. Cytotoxicity was evaluated compared to the control group treated with the stimulant. As a result, it was confirmed that each of samples 1 to 3 and 5 to 12 showed no cytotoxicity at a concentration of 70 ug/mL or less.

<Example 3> Confirmation of anti-inflammatory and antibacterial effects

1. Confirmation of anti-inflammatory effect

(1) Inhibition of nitric oxide production

NO assay was performed to investigate the effect on nitric oxide production, which is one of free radicals and is known to play an important role in causing inflammation. After the cultured RAW 264.7 cells are adjusted to 1.0x10 ¹⁰ cells/mL using DMEM medium, 96 well plates are inoculated, and samples 1 to 4 and sample 13 (samples 1 and 3 are mixed at a weight ratio of 1:1 to form). ) Each and a new medium containing LPS (1 µg/mL) was treated at the same time and cultured for 24 hours (the final concentration of each of Samples 1 to 4 and Sample 13 was 70 µg/mL). The amount of NO generated was measured in the form of NO ₂ ⁻ present in the cell culture solution using a Grease reagent. After 100 μL of the cell culture supernatant and 100 μL of the Grease reagent were reacted for 10 minutes, absorbance was measured at 540 nm using an ELISA reader. The amount of NO generated is compared with sodium nitrite (NaNO ₂ ) as a standard, and the results are shown in Table 1. Looking at Table 1, it can be seen that samples 1 to 4 and 13 both reduce the amount of NO production, and it can be confirmed that sample 3 has the smallest amount of NO production in hwangchil tree extract. It can be seen that the anti-inflammatory effect can be increased. In addition, considering that Sample 13 has the best anti-inflammatory effect, it can be seen that the anti-inflammatory effect can be further improved by mixing the camphor tree extract and hwangchil tree extract in a weight ratio of 1:1.

NO production rate (%) Unprocessed 0 LPS only 100 LPS + Sample 1 79 LPS + Sample 2 89 LPS + Sample 3 75 LPS + Sample 4 94 LPS+sample 13 68

(2) Pro-inflammatory cytokines (TNF-α, IL-6) production inhibition

After the cultured RAW 264.7 cells were adjusted to 1.0×10 ¹⁰ cells/mL using DMEM medium, inoculated and cultured in 96 well plates, samples 1 to 4 and 13 (samples 1 and 3 in a weight ratio of 1:1) The mixture was formed) and cultured by simultaneously treating each medium and a new medium containing the stimulus LPS (1 ug/mL) (final concentrations of each of samples 1 to 4 and 13 were 70 µg/mL). After incubation, the culture medium was centrifuged to measure the amount of pro-inflammatory cytokines produced in the supernatant, and the results are shown in Table 2. The pro-inflammatory cytokines were quantified using a mouse enzyme-linked immnunosorbent assay (ELISA) kit (R&D Systems Inc., Minneapolis, MN, USA), and the r2 value of the standard curve for the standard was 0.99 or higher. Looking at Table 2, in the hwangchil tree sample, it can be seen that the production of TNF-α and IL-6 increases as samples 3, 2, and 4 are used. It can be seen that it can be increased, and Sample 13 shows that Sample 13 has the best anti-inflammatory effect, so that the anti-inflammatory effect can be further improved by mixing camphor extract and hwangchil tree extract in a weight ratio of 1:1. You can.

TNF-α
Production rate (%) IL-6
Production rate (%) Unprocessed 0 0 LPS only 100 100 LPS + Sample 1 83 79 LPS + Sample 2 90 89 LPS + Sample 3 79 72 LPS + Sample 4 95 93 LPS+sample 13 71 67

2. Confirmation of antibacterial effect

(1) 10 ⁹ cells / ml of the test strain (Yellow Staphylococcus aureus) was cultured in the medium, and the cultured test strain was treated with each of samples 1 to 4 and 13 at a concentration of 1 mg/mL, followed by growth of the test strain. The time was observed, and the absorber was measured at 600 nm to measure the antibacterial activity against the test strain, and the results are shown in Table 3.

(2) In Table 3, it can be seen that both samples 1 to 4 and 13 have antibacterial activity, and in Antichil extract, Sample 3 shows the best antibacterial activity, and Sample 13 in which samples 1 and 3 are mixed is anti-bacterial. It can be seen that unlike the inflammatory effect, it does not exhibit an improved synergistic effect.

Sample 1 Sample 2 Sample 3 Sample 4 Sample 13 Antibacterial activity (%) 86 80 89 75 86

<Example 4> Confirmation of wrinkle improvement effect

(1) Cell proliferation effect

The cultured HDFn was inoculated and cultured in a 96 well plate to be 1.0x10 ¹⁰ cells/ml, replaced with DMEM medium not containing FBS, samples 5 to 12 were added, and cultured for 24 hours (samples 5 to 12, respectively). Final concentration is 70 μg/mL). After incubation, 10 μl of a solution of MTT (3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyl tetrazolium: 5 mg/ml) is added, and after 6 hours of reaction, the reaction solution is removed. After absorbing the stained cells by adding 100 µL of DMSO to each well, absorbance at 570 nm was measured. Cell proliferation is calculated and shown in Table 4 (Cell proliferation (%) = (OD of sample-OD of control) / OD of control × 100), it can be seen that all of the samples 5 to 12 improve the cell proliferation, sample It can be seen that when using Sample 12 formed by mixing 5, 6, and 9 in a weight ratio of 1:1:1, it is possible to increase the wrinkle improvement effect than using each extract for improving wrinkles.

Unprocessed Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 Cell proliferation (%) 0 28 31 30 31 27 29 27 43

(2) Collagen synthesis ability After incubating and incubating the cultured HDFn at 1x10 ¹⁰ cells/ml in a 96 well plate, replacing it with DMEM/F12 (3:1) medium without FBS, and adding samples 5 to 12 And cultured for 24 hours (final concentration of each of 5 to 12 samples was 70 µg/mL). Thereafter, the medium supernatant was collected and the amount of procollagen was measured using a Procollagen type 1 C-peptide (PIP) EIA kit, and the results are shown in Table 5. Looking at Table 5, it can be seen that Sample 12 has a larger amount of collagen 1 than Samples 5 to 11. Through this, it can be seen that when using Samples 12 formed by mixing Samples 5, 6, and 9 in a weight ratio of 1:1:1, it is possible to increase the wrinkle improvement effect than using each extract for improving wrinkles.

Unprocessed Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 collagen 1
content(%) 100 126 131 128 130 133 130 129 150

<Example 5> Skin patch evaluation (exfoliation, wrinkle improvement)

1. The cosmetic composition was prepared in the composition ratio of Table 6 below.

content(%) ingredient Sample 1 ~ 4, 13 Sample 5 ~ 12 glycerin antiseptic Spices Purified water Composition 1 1 (sample 1) 1 (sample 12) 2 0.02 0.4 Balance Composition 2 1 (sample 2) 1 (sample 12) 2 0.02 0.4 Balance Composition 3 1 (sample 3) 1 (sample 12) 2 0.02 0.4 Balance Composition 4 1 (sample 4) 1 (sample 12) 2 0.02 0.4 Balance Composition 5 1 (sample 13) 1 (sample 12) 2 0.02 0.4 Balance Composition 6 1 (sample 1) 0 2 0.02 0.4 Balance Composition 7 1 (sample 1) 1 (sample 5) 2 0.02 0.4 Balance Composition 8 1 (sample 1) 1 (sample 6) 2 0.02 0.4 Balance Composition 9 1 (sample 1) 1 (sample 7) 2 0.02 0.4 Balance Composition 10 1 (sample 1) 1 (sample 8) 2 0.02 0.4 Balance Composition 11 1 (sample 1) 1 (sample 9) 2 0.02 0.4 Balance Composition 12 1 (sample 1) 1 (sample 10) 2 0.02 0.4 Balance Composition 13 1 (sample 1) 1 (sample 11) 2 0.02 0.4 Balance Composition 14 1 (sample 1) 1 (sample 12) 2 0.02 0.4 Balance

2. Appearance evaluation

After storing the compositions 1 to 14 at room temperature for 2 days, as a result of checking the appearance, a change in color was observed in the composition 4, but the rest of the compositions did not change color.

3. Evaluation of exfoliation efficacy

It targets 15 women in their 40s, divides 15 people into 5 groups, applies compositions 1 to 5 to the face, and then attaches and exfoliates keratin measuring tape for 30 seconds before and after use of the composition and analyzes to exfoliate. The removal rate was calculated and shown in Table 7 (% exfoliation (%)=(average density of keratin before using the cosmetic composition-average density of keratin after using the cosmetic composition)/average density of keratin before using the cosmetic composition×100). Looking at Table 7, it can be seen that the composition 1 using the camphor tree extract has excellent exfoliation effect.

Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Exfoliation rate (%) 30 12 14 16 23

4. Evaluation of wrinkle improvement effect

For 27 women in their 40s, 27 were divided into 9 groups, and compositions 6 to 14 were used on the face for 3 weeks each morning and evening on the face (wet the composition on the fiber sheet and then peeled the sheet) The skin wrinkle improvement effect around the eyes was measured using a skin wrinkle meter (Skin Visiometer SV 600 FireWire, CK Electronic GmbH), which is attached to the diseased area for a certain period of time. The relative reduction ratio (%) was calculated by relative comparison of the average depth of wrinkles before and after the composition was used, and the relative reduction ratio (%) based on composition 6 is shown in Table 8 (relative reduction ratio (%) = (Use composition X) Reduction rate when using-Reduction rate when using composition 6) / Reduction rate when using composition 1) x 100), X is 6 to 14). Looking at Table 8, it can be seen that all of the compositions 7 to 14 have a remarkable wrinkle improvement effect.

Composition 6 Composition 7 Composition 8 Composition 9 Composition 10 Composition 11 Composition 12 Composition 13 Composition 14 Relative
Decrease rate (%) 0 39 43 39 38 43 39 41 59

In the above, the applicant has described the preferred embodiments of the present invention, but these embodiments are only one embodiment for implementing the technical concept of the present invention and any modification or modification of the present invention as long as the technical concept of the present invention is implemented It should be construed as being within the scope.

Claims (4)

Contains camphor extract and extracts for wrinkle improvement,
The camphor extract is obtained by mixing the camphor leaves with ethanol, after a certain period of time, removing the dry matter, concentrating the extracted extract under reduced pressure and freeze-drying,
The extract for improving wrinkles is a cosmetic composition characterized in that it comprises any one or more selected from the group consisting of Gin Giananam extract, persimmon tree extract, Dampalsu extract, barberry extract and oak tree extract. According to claim 1,
The cosmetic composition further comprises a hwangchil tree extract,
The camphor tree extract and hwangchil tree extract are mixed in a weight ratio of 1:1,
The hwangchil tree extract mixes the hwangchil obtained from the hwangchil tree with an extraction solvent to form a mixed solution and stir, and the mixture is dissolved in an extract solvent by standing at a constant temperature for a period of time after the mixture stirring step. It is obtained through an evaporation step of separating the layer into parts and evaporating the extraction solvent using a rotary evaporator by separating the supernatant, which is the part dissolved in the extraction solvent, in the separation step, using a rotary evaporator under reduced pressure.
A cosmetic composition characterized in that the mixed solution is ultrasonicated and stirred in the mixing and stirring step. delete According to claim 1,
The extract for improving wrinkles includes extract of Gin Giananam, persimmon tree extract and oak tree extract,
The cosmetic composition, characterized in that the Gin Giananam extract, persimmon tree extract and oak tree extract are mixed in a weight ratio of 1:1:1.