KR101754463B1 - COSMETIC COMPOSITIONS CONTAINING the plants extract of Cyperaceae AND PREPARING METHOD OF THE SAME - Google Patents

Abstract

The cosmetic composition of the present invention and a method for producing the same provide a cosmetic composition having an anti-inflammatory and antioxidant activity, and having an anti-inflammatory activity and antioxidative function,

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a mushroom extract and a plant extract, and a method for producing the same. BACKGROUND ART [0002] COSMETIC COMPOSITIONS CONTAINING THE PLANT EXTRACT OF CYPERACE AND AND PREPARING METHOD OF THE SAME [

The present invention relates to a cosmetic composition and a method for producing the same, and more particularly, to a cosmetic composition having an inflammation-improving and antioxidative function and containing Cyperaceae plant extract, more specifically, .

Inflammation is one of the defenses of the body against physical stimuli, chemical stimuli, bacterial infections, etc. It is one of the mechanisms to remove external substances and regenerate damaged areas. Once an inflammation-related stimulus is applied, vasoactive substances such as histamine, serotonine, bradykinin, prostaglandin, hydroxyeicosatetraenoic acid (HETE), leukotriene And the vascular permeability is increased to cause inflammation.

Monocytes are transformed into macrophages that are activated by bacterial infection and act as phagocytic cells. Macrophages induce inflammation by secretion of NO, prostaglandin E2, and proinflammatory cytokines, and regulatory cells important in both inflammation and immune response In order for macrophages to function in this way, they must undergo an activation process. LPS (lipopolysaccharide), one of the cell wall components of Gram-negative bacteria and well known as endotoxin, is the most well-known external factor involved in macrophage activation. Particularly, in monocytes and macrophages such as RAW 264.7 cells, proinflammatory cytokines such as TNF-α, IL-6 (interleukin-6) and IL-1β (interleukin- -inflammatory cytokine secreted by LPS (lipopolysaccharide) in the inflamed area. When LPS stimulates macrophages, nitric oxide (NO) is produced in the process of converting L-arginine into L-citrulline by an enzyme called iNOS (inducible nitric oxide synthase) Phagocytes produce NO.

In mammals, NO is synthesized by three kinds of NO synthase (NOS): nNOS (neuronal NOS), eNOS (endothelial NOS) and iNOS (inducible NOS). Among these, NO produced by nNOS and eNOS is produced for normal biological function, and the concentration in tissues is kept low to a certain level. However, the amount of NO produced by iNOS is excessively high, and it is harmful to living organisms such as pathological vasodilation, cytotoxicity and tissue damage. In addition, prostaglandin E2 (PGE2), an inflammatory factor, stimulates phospholipid, which is a constituent of cell membrane, stimulated by LPS, and arachidonic acid liberated by an enzyme called phospholipase A2 causes catalytic action of an enzyme called COXs (cyclooxygenase) And is induced to induce an inflammatory reaction. COX is classified as COX-1 and COX-2. COX-1 acts on normal biological functions such as platelet formation, gastric wall protection and maintenance of renal function in the body. COX-2 synthesizes PHE2 as an inflammatory mediator. PHE2 is known to be deeply involved in the development of cancer, such as promoting inflammation (pain, fever, etc.), immune response, and angiogenesis. Inhibition of iNOS is mostly due to its mechanism of action being linked to inhibitors of COXs. Therefore, iNOS inhibition materials that are relatively easy to search are discovered and developed as anti-inflammatory materials.

On the other hand, non-steroidal anti-inflammatory drugs (NSAIDs), including selective COX-2 inhibitors, have anti-inflammatory, antipyretic and analgesic effects. However, the long-term use of these drugs can sometimes lead to serious side effects. For example, secondary anemia due to gastrointestinal peptic ulcer bleeding, platelet function suppression, inhibition of induction of labor, side effects to the kidney, liver damage, hypersensitivity.

In order to overcome the side effects of these drugs, natural products such as plants are more preferable as a safer material. In this regard, researches are being actively carried out to find and use the anti-inflammatory materials in natural products.

The antioxidant function inhibits or slows down the oxidation reaction occurring in the body. Oxidation reaction in the body induces aging and cell transformation to generate cancer cells. Therefore, the antioxidant activity is a preventive measure to suppress the negative reaction in the body, and the public is also interested.

In the body, mitochondria within the cell, which is the main function of ATP production, generate free radicals, a byproduct of respiration, which is unstable, and is accompanied by other substances such as lipids, nucleic acids and proteins, It reacts to the enemy. Therefore, if we can prevent the generation of these free radicals, we can prevent the oxidation of the body, and indirectly, we can prevent aging and cancer. These antioxidant effects are difficult to experiment with free radicals occurring in the human body and use DPPH and ABTS radicals as biomarkers. The DPPH free radical scavenging activity assay is one of the methods for measuring the antioxidant activity of a sample. The DPPH free radical scavenging activity assay is a method for measuring the antioxidative activity of DPPH (1,1-diphenyl-2-picyl-hydrazyl) This method of decolorizing antioxidant activity is a widely used method because it can measure antioxidative activity in a relatively short time and has a high correlation with the antioxidant activity of plants.

For example, there are a few barley fores and a thousand nine fores. Carex pumila Thunb ) is a perennial plant of the monocotyledonous plant river subspecies in Korea, Japan, Taiwan, China, Russia, New Zealand, North America and other East Asian countries and Australia. It is a plant commonly found in the sandy beaches. Was not. No studies have been reported on the anti-inflammatory effect of this plant. Carex scabrifolia Steud is a typical perennial plant that grows in salt marshes in coastal and estuarine areas as a perennial plant growing on a marsh of the sea as a zooplankton ( Chrysanthemum sp. Biology Journal Vol. 20, No. 1 2002 pp.25-34). It is characterized by small overall as compared to the big date of the first day. It grows wild throughout Korea and is distributed in Taiwan, Russia, Japan, and China. No studies have been reported on the effect of improving the inflammation of this plant.

An object of the present invention is to inhibit the production of NO has an improved skin-inflammatory effects, can take skin antioxidant effect the free radical scavenging activity, cyperaceae there are no problems with the stability of the plant extract (Cyperaceae) barley little as plant extracts, and more particularly An antioxidant functional cosmetic composition, and a method for preparing the same.

In order to achieve the above object, the cosmetic composition according to an embodiment of the present invention contains a natural anti-inflammatory component including a Cyperaceae plant extract.

The cosmetic composition may contain 0.001 to 20% by weight of the extract.

The cosmetic composition has the function of anti-inflammation and antioxidation and can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, , An essence, a nutritional essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

A method of manufacturing a cosmetic product according to another embodiment of the present invention is a method for producing a cosmetic product by extracting a quercetus and a plant with an extracting solvent selected from the group consisting of alcohol having 1 to 5 carbon atoms, hexane, chloroform, alcohol, water, The process of preparing plant extracts; And a process for producing a cosmetic product by adding an aqueous solution or an emulsion to the extract of Zygoda mushroom.

The preparation process of the mushroom and plant extracts comprises: a pretreatment step of drying mushrooms and plants to prepare dried powder of mushrooms and plants; An extracting step of adding the extracting solvent at a weight of 3 to 20 times the dry weight to the dried powder of the herbaceous plant and conducting extraction at an extraction temperature of 20 to 120 ° C for an extraction period of 30 minutes to 10 days; And removing the solid component from the extract solution of the mushroom plant to obtain a mushroom and plant extract solution.

Hereinafter, the present invention will be described in more detail.

The inventors of the present invention have investigated inflammation inhibitory effects on various plant extracts. As a result, it has been found that the extracts of Zygomycorrhizae extracts, which have not been known to have such an effect, have a NO production inhibitory action and DPPH free radical scavenging activity, It has been confirmed that it can be used as a functional cosmetic product when applied as a cosmetic product, and thus the present invention has been accomplished.

The cosmetic composition according to an embodiment of the present invention contains a natural anti-inflammatory component including a myrtle plant extract.

The cosmetic composition contains a mushroom extract and has antioxidant and anti-inflammatory functionality. The herbaceous plant may be a little barley or chrysanthemum.

The mushroom and plant extract may be obtained by drying and crushing leaves, stem, roots, flowers, fruits, plant outbreaks, or a mixture thereof, and extracting the mushroom and plant extracts using an extraction solvent. The herbaceous plants may be, for example, barley syrup, sunflower syrup, or a mixture thereof.

The dried and crushed herbivorous plants may be leaves, stems, roots, flowers, or a mixture thereof, and the drying may be carried out in a known manner to such an extent that useful components of the herbicidal plants are not destroyed, For example, it can be carried out by a method of natural drying in the shade. In addition, it is preferable that the crushing is performed after crushing to such an extent that the useful components of the moss and herbaceous plant can be sufficiently extracted in the subsequent extraction process. The drying and crushing steps may be carried out in reverse order or repeatedly as required.

The extraction may be carried out by, for example, hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like, preferably by a cold extraction method.

The extraction is carried out using a solvent, and the solvent may be an alcohol having 1 to 5 carbon atoms, hexane, chloroform, hydrated alcohol, acetone, ethyl acetate ester, alcohol, water and mixtures thereof, preferably water, methanol, Ethanol, alcohol, and mixtures thereof.

The extraction is carried out at a temperature of 20 to 100 ° C, preferably 20 to 70 ° C, for 30 to 10 days, preferably 3 to 20 times, preferably 3 to 10 times, , Preferably 1 hour to 1 day, and the extract may be continuously extracted from 1 to 5 times, preferably 2 to 3 times, including the dried and crushed product of the above plant To obtain an extract. The extraction may also be carried out by extracting at an extraction temperature of 2 to 20 ° C, preferably 4 to 16 ° C, for an extraction time of 1 minute to 7 days, preferably 8 hours to 4 days, And the lysate may be continuously extracted once to five times, preferably twice to three times, to obtain a liquid-phase extract.

The liquid extract of Zygoschus japonica can be separated from Zygosaccharum plants and decomposed by filtration, and then concentrated or dried. For example, the above-mentioned liquid phase mushroom and plant extract may be a concentrated liquid obtained by concentration under reduced pressure at 20 to 100 ° C, preferably 40 to 70 ° C by a vacuum rotary condenser, and the liquid mushroom and plant extract is dried to obtain a powdered mushroom Extracts may also be obtained. The concentrated or powdered mushroom and plant extracts can be used as needed in water, alcohols or mixed solvents thereof. In addition, the above-mentioned liquid extract of Zygoda mushroom, its concentrate or powder thereof may be an extract obtained by fractionation by nucleic acid or ethyl acetate or fractional stroke of the fraction.

The extracts of the mushroom extract inhibit the expression of iNOS, which is an inflammation mediator, and inhibit the expression of NO produced by the enzyme, thus being useful as a pharmaceutical composition for antiinflammatory activity by containing a component useful for the prevention and treatment of inflammation related diseases And preferably can be used as a pharmaceutical composition for the prevention or treatment of inflammatory diseases, and can be widely applied to inflammatory diseases.

The extract of Zygoda mushroom may be contained in the cosmetic composition in an amount of 0.001 to 20.0% by weight. If the content is less than 0.001% by weight, the effect of the present invention may be insufficient. If the content is more than 20.0% by weight, formulation may be difficult. In addition, if the content exceeds 10.0% by weight, the degree of improvement of anti-inflammatory and antioxidant functionalities may be insignificant, and the extract of Zygoda mushroom may be contained in the cosmetic composition in an amount of 0.001 to 10.0% by weight. In this case, the weight of the mushroom and plant extract is based on the dried mushroom extract of the mushroom and the plant extract.

The cosmetic composition of the present invention may contain, in addition to the above-mentioned extract of Zygoda-japonica, plant extracts, cosmetics, pharmaceuticals and other components used in external skin preparations such as other whitening agents, moisturizing agents, antioxidants, oily components, ultraviolet absorbers, surfactants, Component, colorant, aqueous component, water, various skin nutrients, and the like can be appropriately blended as required.

Other examples include metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate and gluconic acid, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice extract, gabridine , Hydrothermal extract of firethorn fruit, various herbal medicines, drugs such as tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbyl phosphate, ascorbic acid glucoside, arbutin, kojic acid, lucinol , Erragean, and chamomile, saccharides such as glucose, fructose, mannose, sucrose, trehalose, and the like can be appropriately blended.

In addition, the cosmetic composition may further comprise a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

As the water-soluble vitamin, any of vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C and vitamin H may be used, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) are also included in the water-soluble vitamins usable in the present invention. The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Examples of the above-mentioned useful vitamins include any of vitamin A, carotene, vitamin D2, vitamin D3 and vitamin E (d1-alpha tocopherol, d-alpha tocopherol and d-tocopherol) , And derivatives thereof such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbic acid, ac-1-tocopherol acetate, nicotinic acid dl-a tocopherol, DL- pantothenyl alcohol, D-pantonenyl alcohol, Etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method. The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract, and carrageenan, arginic acid, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic composition may be blended with other ingredients usually added to cosmetics, if necessary. Examples of the compounding ingredients that may be added include organic solvents such as a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, A blood circulation promoter, a cold agent, a restriction agent, and purified water.

Examples of the above-mentioned fat-soluble ingredients include ester-based fats, hydrocarbon-based fats, silicon-based fats, fluorine-based fats, animal fats and plant fats and the like. Examples of the ester-based oil include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, But are not limited to, ethylbutyl, isobutylbutyl, butylbutyl, ethylbutylbutyl, isobutylbutyl, isobutylbutyl, isobutylisobutyl, isobutylisobutylisobutyl, isobutylisobutylisobutylisobutylisobutylisobutylisobutyl, Trimethylolpropane triisostearate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl esters of caprylic acid, lauric acid Decyl, hexyl laurate, myristyl dicyl, myristyl myristate, myristine acid ethyl, stearyl stearate, decyl oleate, cetyl ricinoleate, lauryl Wherein the acid is selected from the group consisting of isostearyl isostearate, isostearyl isostearate, isostearyl isostearate, isostearyl isostearate, isostearyl isostearate, isostearyl isostearate, isostearyl isostearate, isostearyl palmitate, octyl stearate, isostearyl stearate, isodecyl oleate, octyldodecyl oleate, Propylene glycol dicaprylate, propylene glycol dicaprylylate, propylene glycol dicaprylylate, propylene glycol dicaprylate, propylene glycol dicaprylylate, propylene glycol dicaprylylate, propylene glycol dicaprylate, Glyceryl triacetate, glyceryl triisostearate, glyceryl triisostearate, octyldodecyl neopentanoate, octanoic acid, glyceryl triisostearate, glyceryl triisostearate, glyceryl triisostearate, glyceryl triisostearate, Octadecanoic acid octyldodecyl, isostearic acid isosetil, isostearic acid isostearyl, isoselic acid, Polyglycerin isostearic acid ester, triisocetyl citrate, triisocylic citrate, triisooctyl citrate, lauric acid lauryl, myristyl lactate, cetyl lactate, octyldecyl lactate, citric acid tri Ethylhexyl stearate, di-2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacate, diisopropyl sebacate, diisopropyl sebacate, , Stearic acid cholesteryl, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl Diethanolamine, diethanolamine, diethanolamine, diethanolamine, diethanolamine, diethanolamine, diethanolamine, diethanolamine, Stearyl hydroxystearate, isostearyl hydroxystearate, stearyl hydroxystearate, isostearyl hydroxystearate, stearyl 12-stearoyl hydroxystearate, ester of 12-stearoyl hydroxystearic acid, and the like. Examples of the hydrocarbonaceous oil include hydrocarbons such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline. Examples of silicone based oils include polymethyl silicone, methyl phenyl silicone, methyl cyclopolysiloxane, octamethyl polysiloxane, decamethyl polysiloxane, dodecamethyl cyclosiloxane, dimethylsiloxane / methyl cetyloxysiloxane copolymer, dimethylsiloxane / methyl stearoxysiloxane copolymer , An alkyl-modified silicone oil, and an amino-modified silicone oil. Examples of the fluorine-based oil include perfluoropolyether and the like. Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, sunflower oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, macadamia nut oil, meadow home oil, egg yolk oil, woji, mayo, mink oil, orange raffia, jojoba oil, candelilla wax, Carnauba wax liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers. Examples of the water-soluble low-molecular moisturizing agent include serine glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (Polymerization degree n = 2 or more), polyglycerin (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like. Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol, cholesterol ester and the like. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, . Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexene copolymer, nitrocellulose, dextrin fatty acid ester, polymer silicone and the like. Examples of the molient agent include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include a nonionic surfactant, an anionic surfactant, and a cationic surfactant. Examples of the nonionic surfactant include self-emulsifying monostearic acid clicerine, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether- We include acid alkanolamide, alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of the anionic surfactant include fatty acid soap,? -Acylsulfonate, alkylsulfonate, alkylallylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkylether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide Alkylsulfosuccinic acid salts, acylated hydrolyzed glycolipid salts, perfluoroalkyl phosphoric acid esters, and the like may be added to the composition of the present invention. . Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, phydistearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Examples of the amphoteric surfactant include carboxybetaine, amidebetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, amideamine, etc. , And the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

Examples of the organic powder include metal soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl- [beta] -alanine calcium, N-lauroyl- [beta] -alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids such as N epsilon-lauroyl-L-lysine, N epsilon-palmitoylglycine, N alpha -pyattoylol nitin, N alpha -raurolyl arginine and N alpha -curable tallow fatty acid acyl arginine; N-acylpolypeptides such as N-lauroylglycylglycine; ? -amino fatty acids such as? -aminocaprylic acid,? -aminorauric acid and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene styrene copolymer, ethylene tetrafluoride, and the like.

Examples of the ultraviolet absorbers include para-aminobenzoic acid, aminopropyl p-aminobenzoate, octyl p-aminobenzoate, ethyleneglycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate, Octyl methacrylate, dipyrromethoxy cinnamate mono 2-ethylhexane glyceryl, para-methoxy methacrylate isopropyl, diisopropyl / diisopropyl cinnamate ester mixture, urocanic acid, urocanic acid Ethyl, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone, 4- tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p-1,3,5-triazine, and 2-benzotriazole.

Examples of the fungicide include hynokitiol, trichloroacetic acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethyl phenol, azulene, salicylic acid, zinc pyrithione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

The cosmetic composition may take the form of a solution, an emulsion, a viscous mixture or the like.

The ingredients contained in the cosmetic composition according to one embodiment of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetics in addition to the above extracts, for example, stabilizers, solubilizers, vitamins, And may contain conventional customary auxiliaries and carriers.

Cosmetics according to one embodiment of the present invention can be manufactured in any formulations conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, hair cosmetics and the like .

Specifically, the cosmetic according to an embodiment of the present invention may be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, , Nutritional essences, packs, soaps, cleansing foams, cleansing creams, body lotions and body cleansers.

In the case of the paste, cream or gel of the formulation of the present invention, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate benzyl alcohol, benzyl benzoate, propylene glycol, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonium, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The method of manufacturing a cosmetic product according to another embodiment of the present invention is a method for producing a cosmetic product, which comprises extracting a quercetus and a plant with an extracting solvent selected from the group consisting of an alcohol having 1 to 5 carbon atoms, hexane, chloroform, The process of preparing the extract; And a process for producing a cosmetic product by adding an aqueous solution or an emulsion to the extract of Zygoda mushroom.

The preparation process of the mushroom and plant extracts comprises: a pretreatment step of drying mushrooms and plants to prepare dried powder of mushrooms and plants; An extracting step of adding the extracting solvent at a weight of 3 to 20 times the dry weight to the dried powder of the herbaceous plant and conducting extraction at an extraction temperature of 20 to 120 ° C for an extraction period of 30 minutes to 10 days; And removing the solid component from the extract solution of the mushroom plant to obtain a mushroom and plant extract solution. The contents of the mushroom, plant extract or cosmetic composition are omitted from the description in the above.

The cosmetic composition of the present invention and a method for producing the same provide a cosmetic having an anti-inflammatory activity and an antioxidative activity, including a natural anti-inflammatory and antioxidant extract, .

FIG. 1 shows the results of the anti-inflammatory activity test in Example 2 using the extract of Zero barley aspartame prepared in Example 1 of the present invention.
FIG. 2 shows the results of the anti-inflammatory activity test in Example 2 using the extract of Zero barley aspartame prepared in Example 1 of the present invention.
FIG. 3 shows the results of the antioxidant activity test in Example 3 using the extract of Zero Barley aspen prepared in Example 1 of the present invention.
4 shows the results of the anti-inflammatory activity test of Example 8 using the chrysanthemum morifolium extract prepared in Example 7 of the present invention.
FIG. 5 shows the results of the anti-inflammatory activity test of Example 8 using the chrysanthemum morifolium extract prepared in Example 7 of the present invention.
FIG. 6 shows the results of the antioxidant activity test of Example 9 using the chrysanthemum morifolium extract prepared in Example 7 of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example  1: little barley forage ( Carex pumila Thunb ) Preparation of extract

Alcoholic extracts of zooplankton were prepared as follows.

Carex from South Korea pumila Thunb ) was immersed in methanol at room temperature (20 to 25 占 폚) for one week to obtain an extract solution obtained by cold precipitation. The extract was filtered and the solvent was distilled off to obtain 1.6 g of a methanol extract of barley aspartame. This extract was dissolved in 0.5% DMSO at 1% by mass to prepare an extract solution of Example 1, and the extract solution was diluted and adjusted in concentration to apply to the following Examples.

Example  2: Anti-inflammatory activity test using extracts of barley aspartame

RAW 264.7 cultured cells were used for iNOS analysis.

RAW 264.7 cells were cultured in DMEM medium at 37 ° C in a CO2 incubator (95% air, 5% carbon dioxide). After culturing on a 96-well microplate for 24 hours, 2.5 μl of the sample solution diluted to 20, 4, 0.8 and 0.16 μg / ml of the extract solution prepared in Example 1, and 2 μl of LPS (1 μg / ml) 1 × 10 ⁵ RAW 264.7 cells per DMEM culture medium. After 24 hours of incubation, NO concentration and iNOS activity were measured to evaluate NO production inhibition and iNOS activity. Specifically, 50 μl of a Griess reagent was injected into each well, and the absorbance at 540 nm was measured. The results are shown in FIGS. 1 and 2. As a positive control, aspirin was applied in the same manner as above. Aspirin used inhibited iNOS activity by about 50% at a concentration of about 3.2 μg / mL and inhibited almost effectively at 100 μg / mL.

As shown in FIGS. 1 and 2, it was confirmed that the extract of barley aspartame lowered NO concentration and iNOS activity in a concentration-dependent manner. In the case of 20 μg / ml of the sample, the stronger anti-aspirin Indicating that it exhibits inflammatory activity.

Example  3: Antioxidant activity test using extracts of barley aspartame

The DPPH free radical scavenging activity test method was used to evaluate the antioxidant activity of the mulberry barley extract prepared in Example 1,

Twenty microliters of TBHQ (positive control), methanol (negative control), and moxaurea extract prepared in Example 1 (concentration of 2000, 1000, 500, 250 and 125 μg / ml, respectively) were injected into each well of a 96-well microplate , 0.2 mM DPPH (1.1-diphenyl-2picyl-hydrazyl, Sigma chemical Co. USA) methanol solution was added to react. The microplate was wrapped with a foil to block the light, reacted at room temperature for 20 minutes, and the absorbance at 517 nm was measured and shown in FIG.

Referring to FIG. 3, it was confirmed that DPPH free radical scavenging was reduced in a concentration-dependent manner in the barley oriental extract, indicating that the barley oriental extract had an antioxidative activity like the existing antioxidative quinone to be.

Hereinafter, examples of the external preparation for skin containing the anti-inflammatory and antioxidative functions of the present invention are prepared. The blended samples were prepared by the ingredients and contents shown in the following table, and the blend amounts in mass% or parts by mass.

Example  4: Preparation of essence using extracts of barley herb

The essence was prepared by the following formulation using the mallow barley forage extract prepared in Example 1 above. Specifically, in the following Table 1, an oily solution in which the components 1 to 13 are sequentially mixed is heated and dissolved at 75 DEG C, and the aqueous solution, in which the components 14 to 21 are sequentially mixed, is heated and dissolved at 75 DEG C, The mixture was stirred at 4000 rpm for 3 minutes to prepare a mixed solution. Thereafter, 22 was added to the mixed solution, and heated for 2 minutes. The mixed solution was mixed with the ingredients of 23 to 27 and emulsified to prepare an essence solution. The essence solution was further stirred at 50 DEG C at 4000 rpm for 2 minutes Followed by cooling to prepare the essence of Example 4.

No. Prescription Content (parts by mass) One Bees wax 0.3 2 Cetyltetheyl alcohol (Lanette O) 0.6 3 Emulsifier (Arlacel-165) 0.5 4 Surfactant (GMS-105) One 5 Surfactants (Arlacel-60) 0.5 6 Shea butter 0.3 7 Preservatives (Propyl paraben, P-P) 0.07 8 Mineral oil (Lily-70) 2 9 Serum (Estasan-3580) 1.5 10 White pigment (TIO) 1.2 11 Softener (Silicone 200F / 100CS) 0.4 12 Emulsifier (Myrj-52) One 13 Softener (Silicone 245) 5 14 Purified water 61.84 15 Metal ion sequestrants (EDTA-2Na (5%)) 0.4 16 Glycerine 8 17 Moisturizing agent (1, 3-butylene glycol) 7 18 Moisturizing agent (Aminocoat) One 19 Preservative (M-P) 0.2 20 Increasing agent (Keltrol-F (1%)) 2 21 Emulsion stabilizer (Carbopol 941 (2%)) 4 22 An acidity control agent (TEA (10%)) 0.8 23 Moisturizing agent (Na-Hyaluronic acid (1%)) One 24 Preservatives (Phenoxy Ethanol) 0.25 25 The extract of Example 1, One 26 Yeast fermentation broth (Galactomyces) 0.02 27 Perfume 0.12

Example  5: Preparation of lotion using extract

The lotion was prepared by using the mallow barley forage extract prepared in Example 1 as shown in Table 2 below. Specifically, the lotion according to one embodiment of the present invention is prepared by dissolving an oil solution in which the components 1 to 11 of Table 3 are successively mixed by heating at 75 ° C and successively mixing the components 12 to 18 at 75 ° C After heating and dissolution, the aqueous phase aqueous solution is mixed at 4000 rpm for 3 minutes. Then, the components of 19 to 24 were mixed and emulsified in such a solution, and the solution was mixed at 4000 rpm (2 minutes) at 50 DEG C and cooled to prepare the lotion of Example 5.

NO. Prescription Content (parts by mass) One Bees wax 0.3 2 Cetyltetheyl alcohol (Lanette O) 0.7 3 Surfactant (GMS-205) One 4 Stearic Acid 0.3 5 Surfactants (Arlacel-60) 0.6 6 Preservatives (P-P, Propyl paraben) 0.07 7 Mineral oil (Lily-70) 4 8 Serum (Estasan-3580) 3 9 Natural Squalane 4 10 Softener (Silicone 200F / 100CS) 0.5 11 Surfactant (Tween-60) 1.1 12 Purified water 65.6 13 Metal ion sequestrants (EDTA-2Na (5%)) 0.2 14 Glycerine 4 15 Moisturizing agent (1, 3-butylene glycol) 3 16 Preservative (Methyl paravene: MP) 0.2 17 Increasing agent (Keltrol-F (1%)) 5 18 Emulsion stabilizer (Carbopol 941 (2%)) 5 19 Acidity modifier, TEA (10%)) One 20 Moisturizing agent (Na-Hyaluronic acid (1%)) One 21 Preservatives (Phenoxy Ethanol) 0.25 22 The extract of Example 1, One 23 Yeast fermentation broth (Galactomyces) 0.02 24 Perfume 0.16

Example  6: Manufacture of Creams using Extracts of Barley Grass

Cream was prepared using the following ingredients as shown in Table 3, using the extract of Zero barley forage extract prepared in Example 1 above. Specifically, the cream according to an embodiment of the present invention comprises heating and dissolving an oily solution obtained by sequentially mixing the components 1 to 13 of Table 3 by heating at 75 ° C, mixing an aqueous solution containing components 14 to 21 in sequence Heat at 75 ℃ to dissolve. The aqueous phase solution was firstly emulsified at 4000 rpm for 3 minutes, 22 components were added to the solution for secondary emulsification at 4000 rpm, the components 23 to 27 were cooled to 50 캜, and the solution was subjected to tertiary emulsification , And cooling the finished solution at < RTI ID = 0.0 > 28 C < / RTI >

NO. Prescription Content (parts by mass) One Increasing agent (Cetanol 70) 1.5 2 Stearyl Alcohol 1.5 3 Surfactant (Arlacel-165) 0.7 4 Stearic acid 0.5 5 Maintaining (Myrj-52) One 6 Surfactants (Arlacel-60) One 7 Preservatives (P-P) 0.1 8 Natural Squalane 4 9 White pigment (TIO) 2 10 Skin Protector (S / W Petrolatum) One 11 Softener (ODM) 2 12 Softener (Silicone 200F / 100CS) 0.3 13 Softener (Silicone 245) 3 14 Purified water 56.83 15 Metal ion sequestrants (EDTA-2Na (5%)) 0.4 16 Glycerine 6 17 Moisturizing agent (1, 3-butylene glycol) 5 18 Moisturizing agent (Aminocoat) One 19 Preservative (M-P) 0.25 20 Increasing agent (Keltrol-F (1%)) 4 21 Emulsion stabilizer (Carbopol 941 (2%)) 5 22 An acidity control agent (TEA (10%)) 1.6 23 Moisturizing agent (Na-Hyaluronic acid (1%)) One 24 Preservatives (Phenoxy Ethano) l 0.1 25 The extract of Example 1, One 26 Yeast fermentation broth (Galactomyces) 0.02 27 Perfume 0.2

All of the cosmetics obtained in Examples 4 to 6 above were subjected to an iNOS assay for evaluating anti-inflammatory activity and a DPPH free radical scavenging activity test for evaluating antioxidant activity, and they were confirmed to have anti-inflammatory and antioxidative effects, respectively.

Example  7: Sun day ( Carex scabrifolia Steud ) Preparation of extract

Alcoholic extracts of Chrysanthemum morifolium, Trichoderma sp. Were prepared by the following method.

60 g of the outgrowth of Carex scabrifolia Steud , which was collected from Korea, was immersed in methanol for one week at room temperature to obtain an extract obtained by cold precipitation. The extract was filtered and the solvent was distilled off to obtain 1.6 g of methanol extract of the first day of the first day. This extract was dissolved in 0.5% DMSO at 1% by mass to prepare an extract solution of Example 1, and the extract solution was diluted and adjusted in concentration to apply to the following examples.

Example  8: Sun day  Anti-inflammatory activity test using extract

RAW 264.7 cultured cells were used for iNOS analysis.

RAW 264.7 cells were cultured in DMEM medium at 37 ° C in a CO2 incubator (95% air, 5% carbon dioxide). After culturing on a 96-well microplate for 24 hours, 2.5 μl of the sample solution diluted to 20, 4, 0.8 and 0.16 μg / ml of the extract solution prepared in Example 1, and 2 μl of LPS (1 μg / ml) 1 × 10 ⁵ RAW 264.7 cells per DMEM culture medium. After 24 hours of incubation, NO concentration and iNOS activity were measured to evaluate NO production inhibition and iNOS activity. Specifically, 50 μl of a Griess reagent was injected into each well, and the absorbance at 540 nm was measured. The results are shown in FIGS. 4 and 5. As a positive control, aspirin was applied in the same manner as above. Aspirin used inhibited iNOS activity by about 50% at a concentration of about 3.2 μg / mL and inhibited almost effectively at 100 μg / mL.

4 and 5, it was confirmed that the chrysanthemum morifolium extract reduced NO concentration and iNOS activity in a concentration-dependent manner. In the case of 20 μg / ml of the sample, the anti-inflammatory effect, which is almost similar to the aspirin used as the positive control, Activity. ≪ / RTI >

Example  9: Sun day  Antioxidant activity test using extract

The DPPH free radical scavenging activity test method was used to evaluate the antioxidative activity of the chrysanthemum morifolium extract prepared in Example 7,

Twenty microliters of TBHQ (positive control), methanol (negative control), and 1,000 day after-treatment extract solutions (concentration of 2000, 1000, 500, 250 and 125 μg / ml, respectively) prepared in Example 1 were injected into each well of a 96-well microplate, And 180 μl of a methanol solution of 0.2 mM DPPH (1.1-diphenyl-2picyl-hydrazyl, Sigma chemical Co., USA) was added to react. The microplate was wrapped with a foil to block the light, reacted at room temperature for 20 minutes, and the absorbance at 517 nm was measured and shown in FIG.

Referring to FIG. 6, it was confirmed that the chrysanthemum sativus extract showed scavenging of DPPH free radicals in a concentration-dependent manner, which indicates that the chrysanthemum sativa extract has an antioxidative activity like the quinone having the antioxidative function.

Hereinafter, examples of the external preparation for skin containing the anti-inflammatory and antioxidative functions of the present invention are prepared. The blended samples were prepared by the ingredients and contents shown in the following table, and the blending amounts were expressed in mass% or parts by mass.

Example  10: Sun day  Preparation of essence using extract

Using the chrysanthemum milt seed extract prepared in Example 7, the essence was prepared as follows. Specifically, in the following Table 4, an oil solution in which the components 1 to 13 are sequentially mixed is heated and dissolved at 75 DEG C, and the aqueous solution, in which the components 14 to 21 are sequentially mixed, is heated and dissolved at 75 DEG C, The mixture was stirred at 4000 rpm for 3 minutes to prepare a mixed solution. Thereafter, 22 was added to the mixed solution, and heated for 2 minutes. The mixed solution was mixed with the ingredients of 23 to 27 and emulsified to prepare an essence solution. The essence solution was further stirred at 50 DEG C at 4000 rpm for 2 minutes Followed by cooling to prepare the essence of Example 10.

No. Prescription Content (parts by mass) One Bees wax 0.3 2 Cetyltetheyl alcohol (Lanette O) 0.6 3 Emulsifier (Arlacel-165) 0.5 4 Surfactant (GMS-105) One 5 Surfactants (Arlacel-60) 0.5 6 Shea butter 0.3 7 Preservatives (Propyl paraben, P-P) 0.07 8 Mineral oil (Lily-70) 2 9 Serum (Estasan-3580) 1.5 10 White pigment (TIO) 1.2 11 Softener (Silicone 200F / 100CS) 0.4 12 Emulsifier (Myrj-52) One 13 Softener (Silicone 245) 5 14 Purified water 61.84 15 Metal ion sequestrants (EDTA-2Na (5%)) 0.4 16 Glycerine 8 17 Moisturizing agent (1, 3-butylene glycol) 7 18 Moisturizing agent (Aminocoat) One 19 Preservative (M-P) 0.2 20 Increasing agent (Keltrol-F (1%)) 2 21 Emulsion stabilizer (Carbopol 941 (2%)) 4 22 An acidity control agent (TEA (10%)) 0.8 23 Moisturizing agent (Na-Hyaluronic acid (1%)) One 24 Preservatives (Phenoxy Ethanol) 0.25 25 The chrysanthemum sinensis extract of Example 7 One 26 Yeast fermentation broth (Galactomyces) 0.02 27 Perfume 0.12

Example  11: Sunflower seed extract  Manufacture of used lotion

Using the chrysanthemum morifolium extract prepared in Example 7, a lotion was prepared as shown in Table 5 below. Specifically, the lotion according to one embodiment of the present invention is prepared by dissolving an oil solution in which the components 1 to 11 of Table 3 are successively mixed by heating at 75 ° C and successively mixing the components 12 to 18 at 75 ° C After heating and dissolution, the aqueous phase aqueous solution is mixed at 4000 rpm for 3 minutes. Thereafter, the components of 19 to 24 were mixed and emulsified in such a solution, and the solution was mixed at 4000 rpm (2 minutes) at 50 DEG C and cooled to prepare the lotion of Example 11.

NO. Prescription Content (parts by mass) One Bees wax 0.3 2 Cetyltetheyl alcohol (Lanette O) 0.7 3 Surfactant (GMS-205) One 4 Stearic Acid 0.3 5 Surfactants (Arlacel-60) 0.6 6 Preservatives (P-P, Propyl paraben) 0.07 7 Mineral oil (Lily-70) 4 8 Serum (Estasan-3580) 3 9 Natural Squalane 4 10 Softener (Silicone 200F / 100CS) 0.5 11 Surfactant (Tween-60) 1.1 12 Purified water 65.6 13 Metal ion sequestrants (EDTA-2Na (5%)) 0.2 14 Glycerine 4 15 Moisturizing agent (1, 3-butylene glycol) 3 16 Preservative (Methyl paravene: MP) 0.2 17 Increasing agent (Keltrol-F (1%)) 5 18 Emulsion stabilizer (Carbopol 941 (2%)) 5 19 Acidity modifier, TEA (10%)) One 20 Moisturizing agent (Na-Hyaluronic acid (1%)) One 21 Preservatives (Phenoxy Ethanol) 0.25 22 The chrysanthemum sinensis extract of Example 7 One 23 Yeast fermentation broth (Galactomyces) 0.02 24 Perfume 0.16

Example  12: Sunflower seed extract  Manufacture of used cream

Using the chrysanthemum morifolium extract prepared in Example 7, a cream was prepared as shown in Table 6 below. Specifically, the cream according to an embodiment of the present invention comprises a step of heating and dissolving an oily solution obtained by sequentially mixing the components 1 to 13 of Table 6 by heating at 75 ° C, Heat at 75 ℃ to dissolve. The aqueous phase solution was firstly emulsified at 4000 rpm for 3 minutes, 22 components were added to the solution for secondary emulsification at 4000 rpm, the components 23 to 27 were cooled to 50 캜, and the solution was subjected to tertiary emulsification And cooling the finished solution at < RTI ID = 0.0 > 28 C < / RTI >

NO. Prescription Content (parts by mass) One Increasing agent (Cetanol 70) 1.5 2 Stearyl Alcohol 1.5 3 Surfactant (Arlacel-165) 0.7 4 Stearic acid 0.5 5 Maintaining (Myrj-52) One 6 Surfactants (Arlacel-60) One 7 Preservatives (P-P) 0.1 8 Natural Squalane 4 9 White pigment (TIO) 2 10 Skin Protector (S / W Petrolatum) One 11 Softener (ODM) 2 12 Softener (Silicone 200F / 100CS) 0.3 13 Softener (Silicone 245) 3 14 Purified water 56.83 15 Metal ion sequestrants (EDTA-2Na (5%)) 0.4 16 Glycerine 6 17 Moisturizing agent (1, 3-butylene glycol) 5 18 Moisturizing agent (Aminocoat) One 19 Preservative (M-P) 0.25 20 Increasing agent (Keltrol-F (1%)) 4 21 Emulsion stabilizer (Carbopol 941 (2%)) 5 22 An acidity control agent (TEA (10%)) 1.6 23 Moisturizing agent (Na-Hyaluronic acid (1%)) One 24 Preservatives (Phenoxy Ethano) l 0.1 25 The chrysanthemum sinensis extract of Example 7 One 26 Yeast fermentation broth (Galactomyces) 0.02 27 Perfume 0.2

All of the cosmetics obtained in Examples 10 to 12 above were subjected to iNOS assay for evaluating anti-inflammatory activity and DPPH free radical scavenging activity test for evaluating antioxidant efficacy, and they were confirmed to have anti-inflammatory and antioxidative effects, respectively.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (6)

A cosmetic composition for anti-inflammation and antioxidation, comprising an extract of Carex pumila Thunb . delete The method according to claim 1,
Wherein the cosmetic composition contains 0.001 to 20% by weight of the extract. The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a foundation, , A cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser. Little barley four seconds (Carex pumila Thunb) an alcohol having 1 to 5 carbon atoms, hexane, chloroform, ethanol, water and a little barley four seconds (Carex pumila Thunb) extracts extracted with any of the extraction solvent is selected from the group consisting of a mixture thereof Manufacturing process; And
And a process for producing a cosmetic product by adding an aqueous solution or an emulsion to the extract. The method for producing an anti-inflammatory and antioxidant cosmetic product according to claim 1, wherein the cosmetic product contains a Carex pumila Thunb extract. [6] The method of claim 5,
A pretreatment step of drying and pulverizing Carex pumila Thunb to prepare a dry powder;
An extracting step of adding the extracting solvent to the dried powder at a weight of 3 to 20 times the dry weight and conducting extraction during an extraction period of 30 to 10 days at an extraction temperature of 20 to 120 ° C to produce an extractive stock solution; And
And removing the solid content from the extraction stock solution to obtain an extract.

Images