KR102217895B1 - Cosmetic composition using Thymus quinquecostatus Celak extract and Dendropanax morbiferus extract - Google Patents

Abstract

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition capable of providing an improved skin improvement effect by containing thyme extract and hwangchil tree extract.

Description

Cosmetic composition using Thymus quinquecostatus Celak extract and Dendropanax morbiferus extract}

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition capable of providing an improved skin improvement effect by containing thyme extract and hwangchil tree extract.

Cosmetics are products used in the human body to add attractiveness by cleansing and beautifying the human body, to brighten appearance, or to maintain or promote the health of skin and hair.As the economy develops and the standard of living increases, as in the following patent documents. Functional cosmetics are being widely developed to provide skin inflammation relief, antioxidant, whitening, and wrinkle improvement effects.

<Patent Literature>

Patent No.0-1550422 (registered on August 31, 2015) "Cosmetic composition containing indigo extract"

However, the conventional cosmetic composition has a problem in that the skin improvement effect is not satisfactory and stability is not guaranteed.

The present invention is to solve the problems of the prior art described above,

An object of the present invention is to provide a cosmetic composition having an improved antioxidant effect by containing thyme extract and hwangchil tree extract.

In addition, an object of the present invention is to provide a cosmetic composition that can compensate for the shortcomings and exert new effects by using a unique extraction method or using additional ingredients.

The present invention is implemented by an embodiment having the following configuration in order to achieve the above object.

According to an embodiment of the present invention, the cosmetic composition according to the present invention is characterized in that it comprises thyme extract and hwangchil tree extract.

According to another embodiment of the present invention, the cosmetic composition according to the present invention is characterized in that it provides an antioxidant effect.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the hwangchil tree extract is characterized in that 100 to 300 parts by weight per 100 parts by weight of the thyme extract are used.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the hwangchil tree extract is characterized in that 200 parts by weight per 100 parts by weight of the thyme extract are used.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the hwangchil tree extract is obtained by drying and pulverizing hwangchil tree leaves, adding an extraction solvent, and filtering and concentrating.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the thyme extract is obtained by drying and pulverizing thyme leaves, adding an extraction solvent, and filtering and concentrating.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, the hwangchil tree extract is mixed and stirred to form a mixed solution by mixing pulverized hwangchil tree after drying into an extraction solvent, and agitating the mixture After the step, a filtration step in which a filtrate is removed by filtration to remove debris, a separation step in which the filtrate is allowed to stand at a certain temperature for a certain period of time after the filtration step, and a layer separation step, and the supernatant after the separation step is separated to use a reduced pressure rotary evaporator. It is characterized in that it is obtained through an evaporation step of evaporating the extraction solvent to extract the Hwangchil tree extract.

According to another embodiment of the present invention, in the cosmetic composition according to the present invention, in the separation step, the filtrate is subjected to ultrasonic treatment using an ultrasonic disperser and then layer separation is performed, thereby improving the extraction efficiency of the Hwangchil tree extract. It is characterized by having.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further comprises a wrinkle improvement extract for wrinkle improvement, and the wrinkle improvement extract is Gingianam extract, persimmon tree extract, juniper extract and cedar tree It characterized in that it comprises any one or more selected from the group consisting of extracts.

According to another embodiment of the present invention, the cosmetic composition according to the present invention further comprises an extract for improving wrinkles for improving wrinkles, and the extract for improving wrinkles includes a gingianam extract, a persimmon tree extract, and a cedar extract, , The ginianam extract, persimmon tree extract and cedar tree extract are mixed in a weight ratio of 1:1:1.

The present invention can obtain the following effects by the above embodiment.

The present invention has an improved antioxidant effect by containing thyme extract and hwangchil tree extract.

In addition, the present invention has the effect of using a unique extraction method or using additional ingredients to compensate for the shortcomings and exert new effects.

Hereinafter, a cosmetic composition containing thyme extract and hwangchil tree extract according to the present invention will be described in detail. Unless otherwise defined, all terms in this specification are the same as the general meaning of the terms understood by those of ordinary skill in the art to which the present invention belongs, and in case of conflict with the meaning of terms used herein, In accordance with the definitions used in the specification. In addition, detailed descriptions of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

The cosmetic composition according to an embodiment of the present invention includes a thyme extract and a hwangchil tree extract. In the cosmetic composition, the thyme extract and the hwangchil tree extract provide an improved antioxidant effect, and the thyme extract and the hwangchil tree extract are each contained in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition. The hwangchil tree extract is preferably used in an amount of 100 to 300 parts by weight per 100 parts by weight of the thyme extract. The thyme ( Thymus quinquecostatus Celak ) is a deciduous semi-shrub belonging to the Lamiaceae family, and is also called thyme, and the hwangchil tree ( Dendropanax morbiferus ) is an evergreen tree of the dicotyledonous plant umbel. Leaves, stems, roots, etc. may be used as the thyme, and leaves, stems, roots, fruits, etc. may be used for the Hwangchil tree. The thyme extract and hwangchil tree extract can be obtained by drying and pulverizing each of thyme and hwangchil tree, adding an extraction solvent, and filtering and concentrating. In addition, the Hwangchil tree extract is a mixing and stirring step of mixing the crushed Hwangchil tree after drying into an extraction solvent to form a mixed solution and stirring, and a filtering step of removing debris by filtering after the mixing and stirring step to obtain a filtrate, After the filtration step, a separation step in which the filtrate is allowed to stand at a certain temperature for a certain period of time to separate layers, and an evaporation step of separating the supernatant in the separation step and evaporating the extraction solvent using a reduced pressure rotary evaporator to extract the Hwangchil tree extract. It is obtained through. Ethanol, purified water, etc. may be used as the extraction solvent, and in the separation step, a supernatant liquid on the upper side, which is a part soluble in the extraction solvent, and a sediment layer on the lower side, in the form of a powder, which are not dissolved in the extraction solvent. . The Hwangchil tree extract obtained through this has an improved antioxidant effect and does not change color for a long time even when exposed to the atmosphere. The Hwangchil tree extract may improve the extraction efficiency of the Hwangchil tree extract which performs layer separation after ultrasonic treatment of the filtrate using an ultrasonic disperser in the separation step, which is one step of the obtaining process. The ultrasonic treatment may be performed using an ultrasonic disperser.

The cosmetic composition may further include a wrinkle improvement extract for improving skin wrinkles. The extract for improving the skin wrinkles may be used any one or more selected from the group consisting of ginianam extract, gamtang tree extract, dampal water extract, juniper juniper extract, and juniper extract, and It is more preferable that the oak tree extract is mixed and used in a weight ratio of 1:1:1. The extract for improving the wrinkles is contained in an amount of 0.001 to 10% based on the total weight of the cosmetic composition. The gingianam extract, persimmons tree extract, dampalm water extract, fupi juniper extract and basswood extract are mixed with gingianam leaves, persimmons persimmons leaves, dampals tree leaves, juniper juniper leaves, and sesame leaves in ethanol, It is obtained by removing the ingredients and concentrating the obtained extract under reduced pressure. The Gyeonggianam is an evergreen perennial plant of the Orchidaceae family, with small flowers blooming and nodes on stems like bamboo, flowers bloom in early spring, growth stops in summer, and flowers have pink and white colors. The persimmon tree is an evergreen small arboreous tree belonging to the persimmon tree family, and its leaves are alternate, thick, oval or similar, and flowers bloom in March and April. Dampalsu is an evergreen arboreous tree belonging to the Dampalaceae family, reaching a height of 20m, hairless, leaves are alternating, lanceolate, thick, with shallow serrates at the edges, flowers bloom in July, white and arranged in a racemic shape, fruits are drupe and ellipsoids. It is 2~2.5cm long and ripens in black purple in September. The pachyderm is an evergreen arboreous tree of the dicotyledonous plant of the tea tree family. The leaves run alternately but gather at the ends of the branches and have a long oval shape in the shape of an inverted lancet shape, a lancet shape, or an egg upside down, and the edge of the leaf is flat, thick and shiny. And petioles are red. The basswood is an evergreen arboreous tree of the dicotyledonous plant Ipanhwa group Buttercup, Camphor tree, grows in the beach sand, reaches 10m in height, small branches are green, leaves are thick, long oval, flat edges, three veins are distinct, and the surface is It is green and the back is white.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any one that can be blended into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. These salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) are also water-soluble vitamins that can be used in the present invention. Included in The water-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme method or a chemical synthesis method.

As the oil-soluble vitamin, any one may be used as long as it can be blended in cosmetics, but preferably, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. And their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, nicotinic acid dl-alpha tocopherol vitamin E, DL-pantotenyl alcohol, D-pantotenyl alcohol, pantotenyl) Ethyl ether, etc.) are also included in the oil-soluble vitamin used in the present invention. The oil-soluble vitamin can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme or chemical synthesis method.

The polymeric peptide may be any one as long as it can be blended in a cosmetic, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are exemplified. The polymeric peptide can be purified and obtained by a conventional method such as a purification method from a culture medium of a microorganism, an enzyme method, or a chemical synthesis method, or can be used after being purified from natural products such as dermis of pigs or cows, silk fibers of silkworms. .

The polymeric polysaccharide may be any one as long as it can be blended into a cosmetic, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be any one as long as it can be blended into a cosmetic, and ceramide, phytosphingosine, sphingoglycolipids, and the like are preferred. The sphingo lipid can usually be purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method or obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be blended into cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc. are exemplified, and carrageenan, arginic acid, sodium arginate purified from these seaweed extracts And potassium arginate are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, other ingredients that are usually blended in the cosmetic composition may be blended. For example, fat and oil components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, Refrigerant agents, restrictive agents, and purified water are mentioned.

Examples of the oil and fat components include ester oils, hydrocarbon oils, silicone oils, fluorine oils, animal oils and vegetable oils. Examples of the ester-based fats and oils include tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid. Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adi Diisopropyl pinate, isoalkyl neopentanoate, tri(caprylic, capric acid) glyceryl, tri2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra2-ethylhexanoate pentaelisli Toll, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isosyl laurate Thearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, seto 2-ethylhexanoate Stearyl, 2-ethylhexanoate stearyl, isostearate hexyl, dioctanoate ethylene glycol, dioleate ethylene glycol, dicaprylic acid propylene glycol, di(caprylic acid) propylene glycol, dicaprylic acid propylene glycol, Neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tricaprylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostea octanoate Reel, isononanoate octyl, neodecanoate hexyldecyl, neodecanoate octyldodecyl, isostearate, isostearic acid isostearyl, isostearate octyldecyl, polyglycerololeic acid ester, polyglycerin isostearic acid ester , Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, horse Diisostearyl acid, 2-ethylhexyl hydroxystearate, 2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacic acid, dioxy sebacic acid Tyl, cholesteryl stearate, cholesteryl isostearate, hydroxystearate cholesteryl, cholesteryl oleate, dihydrocholesteryl oleate, pitsteryl isostearate, pitsteryl oleate, 12-stearate And esters such as isocetyl alloylhydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate. Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and petrolatum. Examples of the silicone fats and oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylsteaoxysiloxane copolymer, Alkyl-modified silicone oils, amino-modified silicone oils, and the like. Examples of the fluorine-based fats and oils include perfluoropolyether. The animal or plant fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, bird flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, cottonseed oil, palm oil, cucuine nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum oil, marker deimia nut oil, meadow home oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba And animal or vegetable fats and oils such as oil, candery wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. Examples of the water-soluble low-molecular moisturizer include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactate, and the like. Examples of the fat-soluble low molecular weight moisturizer include cholesterol and cholesterol esters. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. Can be lifted. Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants. As the nonionic surfactant, self-emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene), sorbitan fatty acid ester, POE sorbit fatty acid ester , POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POEPOP (polyoxyethylene polyoxypropylene) copolymer, POEPOP alkyl ether, polyether modified silicone, lauric acid alkanolamide , Alkylamine oxide, hydrogenated soybean phospholipid, and the like. As the anionic surfactant, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl Amide phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, etc. I can. Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyldimethylbenzyl ammonium chloride, behenyl trimethyl ammonium bromide, Benzalkonium chloride, diethylaminoethyl amide stearate, dimethylaminopropyl amide stearate, lanolin derivative quaternary ammonium salt, and the like. Examples of the amphoteric surfactant are carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. And amphoteric surfactants.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, oxide. Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenolic resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene-styrene copolymer, Organic pigments, such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments, etc. are mentioned.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as sodium zinc cetylate, a zinc laurylate, and calcium laurylate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lizine, N-alpha-paritoylolnitine, N-alpha-lauroylarginine, N-alpha-hardened tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, ethylene tetrafluoride, and the like.

Examples of the UV absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicate, butyl phenyl salicylate, homomentyl salicylate, cinnamic acid Benzyl, paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl/diisopropyl cinnamic acid ester mixture, Urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Examples of the disinfectant include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphylthione, benzalkonium chloride, Photosensitizer No. 301, mononitrowire sodium, undecylenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and lysorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmalic acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogen phosphate.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending ingredients that may be added are not limited thereto, and any of the above ingredients may be blended within a range not impairing the object and effect of the present invention, but preferably 0.01 to 5% by weight based on the total weight, More preferably, it is blended in an amount of 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the shape of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above compounds as an active ingredient. For example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and fragrances And a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, an emulsion, a cream, a foundation, a lotion, a essence, a hair cosmetic, and the like. Specifically, the cosmetic composition of the present invention is a skin lotion, skin softener, moisture lotion, nutrition lotion, massage cream, nutrition cream, hand cream, moisture cream, essence, pack, soap, lotion, milk lotion, gel, ointment, patch, Cleansing foam, body cleanser, astringent, and spray formulations are included. When the formulation of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can. When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether. When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used. When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for describing the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1> Preparation of sample

1. After drying and pulverizing thyme leaves, ethanol was added (using 1000 parts by weight of ethanol per 100 parts by weight of thyme pulverized product), stirred for 24 hours, extracted, filtered and concentrated under reduced pressure to obtain a thyme extract (Sample 1).

2. After drying and pulverizing the washed Hwangchil tree leaves, ethanol was added (using 1,000 parts by weight of ethanol per 100 parts by weight of Hwangchil tree crushed product), extracted for 24 hours by stirring, filtered and concentrated under reduced pressure to extract Hwangchil tree (Sample 2) Got it.

3. After drying and pulverizing the washed Hwangchil tree leaves, ethanol was added (using 1000 parts by weight of ethanol per 100 parts by weight of Hwangchil tree crushed product), stirred for 24 hours, and filtered to remove debris to obtain a filtrate. After standing for a period of time, layer separation into the supernatant which is a part soluble in ethanol and a precipitate layer which is a part that is not dissolved in ethanol, and the extraction solvent is concentrated under reduced pressure in the separated supernatant using a vacuum evaporator to extract Hwangchil tree extract (sample 3) was obtained. After 3 times of water was added to each of Samples 2 and 3 and maintained at room temperature for 3 days, as a result of examining the appearance, it was confirmed that the color of Sample 3 did not change, but the color of Sample 2 changed to darker.

4. After filtration, the filtrate was subjected to ultrasonic treatment for 20 minutes using an ultrasonic disperser, and other conditions were the same as in Example 1 3, except that layer separation was performed to obtain a Hwangchil tree extract (Sample 4). As a result of weighing samples 3 and 4, it can be confirmed that sample 4 weighs 20% more than that of 3, and it can be seen that the extraction amount can be further increased when ultrasonic treatment is performed in the extraction process.

5. Dried and pulverized Gingianam (leaf), Persimmon tree (leaf), Dampalsu (leaf), Fupi juniper (leaf), and sesame tree (leaf) each 250g is immersed in 500ml ethanol for 36 hours to extract, and the extract is depressurized. By evaporation, Gingianam extract (Sample 5), Persimmon tree extract (Sample 6), Dampal water extract (Sample 7), Pachyderm extract (Sample 8), Basswood extract (Sample 9), Samples 5 and 7 were 1: Sample 10 was prepared by mixing at a weight ratio of 1, Samples 5 and 9 were mixed at a weight ratio of 1:1 to prepare Sample 11, and Samples 5, 6 and 9 were mixed at a weight ratio of 1:1:1 to prepare Sample 12 Prepared.

<Example 2> Cytotoxicity evaluation (MTT assay)

MTT assay was performed to determine the degree of toxicity of samples 1 to 12. The cultured RAW 264.7 cells were simultaneously treated with LPS (1 μg/mL) and samples 1 to 12, respectively, and cultured for 24 hours. After the cultivation was completed, 10 μL of MTT solution was added and incubated for 8 hours. After removing the supernatant, DMSO was added, and after shaking well using a plate mixer, the absorbance was measured at 540 nm. Cytotoxicity was evaluated compared to the control group treated with the stimulant. As a result, it was confirmed that each of Samples 1 to 12 did not show cytotoxicity at a concentration of 70 ug/mL or less.

<Example 3> Antioxidant effect evaluation

1. Samples 1 and 2 were mixed at a weight ratio of 1:0.5 to obtain antioxidant sample 1, and samples 1 and 2 were mixed at a weight ratio of 1:1 to obtain antioxidant sample 2, and samples 1 and 2 were mixed at a weight ratio of 1:2 To obtain antioxidant sample 3, samples 1 and 2 were mixed at a weight ratio of 1:3.5 to obtain antioxidant sample 4, samples 1 and 3 were mixed at a weight ratio of 1:2 to obtain antioxidant sample 5, and sample 1 It was used as antioxidant sample 6, and sample 2 was used as antioxidant sample 7.

2. Electron donating ability (EDA)

(1) The electron donating ability of the antioxidant sample was measured by modifying the method of Blois (1958). After adding 10 μL of each antioxidant sample to 90 μL of methanol, 100 μL of 0.15 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was mixed and stabilized at room temperature for 30 minutes, and the absorbance was measured at 517 nm with a UV-vis spectrophotometer. The difference in absorbance between the sample added group and the non-added group was expressed as a percentage (%) to measure electron donating ability (EDA(%) = (1-OD of sample/OD of control)×100). IC ₅₀ (μg/mL) was calculated using the measured electron donating ability and shown in Table 1 below.

Antioxidant Sample 1 Antioxidant Sample 2 Antioxidant Sample 3 Antioxidant Sample 4 Antioxidant Sample 5 Antioxidant Sample 6 Antioxidant Sample 7 IC ₅₀
(μg/mL) 35.21 31.26 24.67 35.43 20.34 38.86 34.55

(2) Looking at Table 1, it can be seen that the antioxidant effect can be improved when using a mixture of 100 to 300 parts by weight of Hwangchil tree extract per 100 parts by weight of thyme extract than when only thyme extract is used or only Hwangchil tree extract is used. I can. In addition, it can be seen that the antioxidant effect can be further improved when the antichil tree extract of Sample 3 is used.

3. Measurement of total polyphenolic compound content

(1) The amount of total polyphenolic compounds in the antioxidant sample was colorimetrically determined according to the Folin-Denis method reported by Taga et al. (1984). 5 mL of distilled water was mixed with 100 μL of each antioxidant target, 1 mL of folin reagent was mixed, 1.5 mL of a 20% sodium carbonate solution was added and mixed, and allowed to stand at room temperature for 2 hours, and the absorbance was measured at 765 nm. The total polyphenol content was calculated as GAE (gallic acid equivalents) by preparing a calibration curve using gallic acid and then quantifying, and the results are shown in Table 2.

Antioxidant Sample 1 Antioxidant Sample 2 Antioxidant Sample 3 Antioxidant Sample 4 Antioxidant Sample 5 Antioxidant Sample 6 Antioxidant Sample 7 Total polyphenol compounds (μg GAE/mL) 395.62 394.00 382.00 378.3 396.7 414.48 350.14

(2) Looking at Table 2, it can be seen that thyme extract, Hwangchil tree extract, and antioxidant samples, which are a mixture of these mixtures at a certain ratio, have a high content of polyphenols. In that the content does not increase, the compounds providing the antioxidant effect contained in each extract are different, and when they are mixed in a certain ratio, it seems that each compound exerts a synergistic effect with each other in the antioxidant effect.

<Example 4> Wrinkle improvement effect evaluation

1. Cell proliferation effect

(1) The cultured HDFn was inoculated into a 96 well plate so as to be 1.0x10 ¹⁰ cells/ml, then replaced with DMEM medium without FBS, samples 5 to 12 were added, and cultured for 24 hours (Samples 5 to 12 Each final concentration is 70µg/mL). After incubation, 10 µl of MTT (3-(4,5-dimethyl-thiazol-2-yl)2,5-diphenyl tetrazolium: 5 mg/ml) solution was added and reacted for 6 hours, and the reaction solution was removed. After lysing the stained cells by adding 100 μl of DMSO to each well, the absorbance at 570 nm was measured. Cell proliferation power was calculated and shown in Table 3 (cell proliferation power (%) = (OD of sample-OD of control) / OD of control × 100).

Untreated Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 Cell proliferation power (%) 0 28 31 30 31 27 29 27 43

(2) Looking at Table 3, it can be seen that both samples 5 to 12 improve cell proliferation, and each wrinkle improvement when using sample 12 formed by mixing samples 5, 6 and 9 at a weight ratio of 1:1:1 It can be seen that the anti-wrinkle effect can be increased than that of using the dragon extract.

2. Evaluation of collagen synthesis ability

(1) After inoculating cultured HDFn to 1x10 ¹⁰ cells/ml in a 96 well plate, replacing it with DMEM/F12 (3:1) medium without FBS, and adding samples 5 to 12 for 24 hours. During the culture (final concentration of each of samples 5 to 12 is 70 μg/mL). Thereafter, the supernatant of the medium was collected and the amount of procollagen was measured using a Procollagen type 1 C-peptide (PIP) EIA kit, and the results are shown in Table 4.

Untreated Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 collagen 1
content(%) 100 126 131 128 130 133 130 129 150

(2) Looking at Table 4, it can be seen that Sample 12 has a higher amount of collagen 1 than Samples 5 to 11. Through this, it can be seen that when using Sample 12 formed by mixing Samples 5, 6 and 9 in a weight ratio of 1:1:1, the wrinkle-improving effect can be increased than that of each wrinkle-improving extract.

3. Skin patch test for wrinkle improvement effect

(1) The cosmetic composition was prepared in the composition ratio shown in Table 5 below.

content(%) ingredient Sample 1 Sample 2 Sample 5 to 12 antiseptic Spices Purified water Composition 1 0.5 One 0 0.02 0.4 Balance Composition 2 0.5 One 0.5 (sample 5) 0.02 0.4 Balance Composition 3 0.5 One 0.5 (sample 6) 0.02 0.4 Balance Composition 4 0.5 One 0.5 (sample 7) 0.02 0.4 Balance Composition 5 0.5 One 0.5 (sample 8) 0.02 0.4 Balance Composition 6 0.5 One 0.5 (sample 9) 0.02 0.4 Balance Composition 7 0.5 One 0.5 (sample 10) 0.02 0.4 Balance Composition 8 0.5 One 0.5 (sample 11) 0.02 0.4 Balance Composition 9 0.5 One 0.5 (sample 12) 0.02 0.4 Balance

(2) Targeting 27 women in their 40s, dividing 27 into 9 groups, and using Compositions 1 to 9 on their faces every morning and evening for 3 weeks (after soaking the composition in a fiber sheet, the The sheet was attached to the skin disease area for a certain period of time), and the effect of improving the skin wrinkles around the eyes was measured using a skin visiometer (Skin Visiometer SV 600 FireWire, CK Electronic GmbH), a fine wrinkle measuring instrument. The reduction rate (%) was calculated by relative comparison of the average depth of wrinkles before and after using the composition, and the relative reduction rate (%) based on the composition 1 is shown in Table 6 (relative reduction rate (%) = (composition X is used) Reduction rate when using-Reduction rate when using composition 1) / Reduction rate when using composition 1) × 100), X is 1 to 9).

Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composition 9 Relative
Reduction rate (%) 0 31 34 28 27 31 41 37 57

(3) Looking at Table 6, it can be seen that all of the compositions 2 to 9 are effective in improving wrinkles.

In the above, the applicant has described various embodiments of the present invention, but such embodiments are only one embodiment that implements the technical idea of the present invention, and any changes or modifications may be made as long as the technical idea of the present invention is implemented. It should be construed as falling within the scope of

Claims (10)

Contains thyme extract and Hwangchil tree extract for antioxidant effect, and wrinkle improvement extract for wrinkle improvement,
The anti-wrinkle extract includes gingianam extract, persimmon tree extract and bass tree extract,
The cosmetic composition, characterized in that the ginianam extract, persimmon tree extract, and cedar tree extract are mixed in a weight ratio of 1:1:1. delete The method of claim 1, wherein the Hwangchil tree extract
Cosmetic composition, characterized in that 100 to 300 parts by weight is used per 100 parts by weight of the thyme extract. The method of claim 1, wherein the Hwangchil tree extract
Cosmetic composition, characterized in that 200 parts by weight per 100 parts by weight of the thyme extract is used. The method of claim 1, wherein the Hwangchil tree extract
A cosmetic composition, characterized in that obtained by drying and pulverizing the leaves of Hwangchil tree, adding an extraction solvent, and filtering and concentrating. The method of claim 1, wherein the thyme extract
A cosmetic composition, characterized in that obtained by drying and crushing thyme leaves, adding an extraction solvent, and filtering and concentrating. The method of claim 1, wherein the Hwangchil tree extract
After drying, the pulverized Hwangchil tree is mixed with an extraction solvent to form a mixed solution and stirred, followed by filtration to remove debris to obtain a filtrate, and a filtrate after the filtration step. Cosmetics, characterized in that it is obtained through an evaporation step of separating layers by standing at a certain temperature for a certain period of time, and separating the supernatant after the separating step and evaporating the extraction solvent using a reduced pressure rotary evaporator to extract the Hwangchil tree extract. Composition. The method of claim 7,
A cosmetic composition, characterized in that it is possible to improve the extraction efficiency of the Hwangchil tree extract by performing layer separation after ultrasonic treatment of the filtrate using an ultrasonic disperser in the separation step. delete delete