KR20220049675A - Cosmetic composition comprising extracts of burdock seed and Torreya grandis aril for improving wrinkles, elasticity or moisturizing the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for wrinkle alleviation, elasticity improvement, or moisturization of the skin, comprising a mixed extract of Arctium minus and Torreya nucifera arils as an active ingredient, wherein the extract increases collagen type 1 gene expression to induce collagen biosynthesis, thereby having effects of improving skin elasticity and alleviating wrinkles, and increases collagen type 2 expression to exhibit an excellent moisturizing function.

Description

Cosmetic composition comprising extracts of burdock seed and Torreya grandis aril for improving wrinkles, elasticity or moisturizing the skin

The present invention relates to a cosmetic composition for improving skin wrinkles, improving elasticity, or moisturizing the skin, comprising a mixed extract of Torreya grandis aril as an active ingredient.

The skin is a body organ that is in close contact with the external environment within the human body and has a function of protecting the inside of the human body, and can be broadly divided into three types: the epidermis, the dermis, and the hypodermis. The skin is a complex organ composed of cells with very diverse functions and materials with appropriate properties. The dermis layer maintains the original skin elasticity because elastic solid materials (fibers) and viscous liquid materials are combined. . Thus, the skin exhibits a reaction due to its unique physical properties to mechanical tension, which is called viscoelasticity. The reason that the skin has such viscoelasticity is because collagen fibers and elastin fibers form a unique three-dimensional structure in the matrix of proteoglycans. In general, as we age, the function of the skin deteriorates and atrophic changes occur due to ultraviolet rays, pollution, and stress, and the number of skin cells decreases or the thickness of the skin decreases. In this process, the elastic fibers of the skin are deformed, and the elasticity of the skin is reduced or the skin is stretched.

Collagen is a major component of the skin, among which type I collagen accounts for 80% and type III collagen accounts for 15%. When the skin is exposed to ultraviolet rays for a long time, a large amount of active oxygen is generated in the skin, and this results in the expression of matrix metalloproteinase-1 (MMP-1), an enzyme that degrades collagen among matrix metalloproteinases (MMPs) The activity is promoted and collagen synthesis is lowered, which in turn leads to a decrease in skin elasticity and the creation of wrinkles. Therefore, in discovering new materials for the prevention and treatment of skin aging, it can be a good strategy to find substances that inhibit the expression and activity of MMP-1 and promote collagen synthesis (Journal of Biological Chemistry, 274, 21491-21494) , 1999; Journal of Investigative Dermatology, 107(3), 404-11, 1996)

The human body is mostly composed of water, which plays an important role in maintaining life. As normal skin contains 70% of moisture in the body and 20% in the stratum corneum, it supports the elasticity and flexibility of the skin. It is known that when the moisture in the stratum corneum is 10% or less, the skin becomes dry and causes skin troubles. The moisture balance of the skin is regulated by the epidermis. Although the stratum corneum of the epidermis is a pile of dead cells, it plays a very important role in maintaining moisture in the skin. The amount of water in the stratum corneum produced by regular keratinization is always maintained at 10-20%. This is because of the sebum film, which is a mixture of sweat and sebum, protecting the skin from the outside and preventing moisture from evaporating from the stratum corneum. Natural Moisturizing Factor (NMF) and dermal lipids hold and maintain moisture. The sebaceous membrane, NMF and dermal lipids work together to retain moisture in the skin. NMF is a hydrophilic, hygroscopic substance that is generated during the keratinization process and plays an important role in moisturizing the skin. It is made of amino acids and holds moisture in the horny layer. In addition, 2-pyrrolidine-5-carboxylic acid (PCA) in NMF is particularly important. It forms a layer with the matrix filled between each cell and maintains moisture inside (Journal of the Skin Barrier Society, 4, 71-80, 2002).

It has been reported that the amount of hyaluronic acid in human skin decreases with aging, and this is considered to be one of the direct causes of the decrease in skin elasticity and moisture content according to aging. The synthesis of hyaluronic acid is carried out by HAS (Hyaluronic Acid Synthase), and the moisturizing effect of the raw material can be confirmed by increasing the expression of HAS-2 (Journal of Drugs in Dematology, 6, s20-4, 2007; Experimental Dermatology, 18(12), 1028-1035, 2009).

Burdock seed ( Arctium lappa seed) is the dried seed (fruit) of burdock, a plant in the Asteraceae family. Burdock grows and is planted in the mountains and fields of various places. Pick ripe fruits in autumn, dry them in the sun, and tap to remove seeds. The taste is spicy and the nature is flat. It acts on menopause and gastric menopause, and smooths anti-jin by removing and detoxifying the wind.

Torreya nucifera in Korea and Torreya grandis in China. In both types of trees, a hard seed coat is surrounded by thick aril like yew or fern. Aerial skin is a seed coat made by the development of parts other than the epidermis of the pear. The seed coat of Bija is discarded after removing the seed and seed coat from the fruit of Bija in both China and Korea. Recently, as research for processing food by-products or wastes is being actively conducted, the need to apply this kind of research to non-agaric skin is emerging.

Accordingly, the present inventors have completed the present invention by confirming that the mixed extract of woobangja and Torreya grandis agaric bark has wrinkle improvement, elasticity improvement and moisturizing effects on the skin.

Republic of Korea Patent Registration No. 10-0586307 (Title of the invention: Cosmetic composition for improving skin wrinkles and elasticity containing Bija extract as an active ingredient, Applicant: ST Cinara Co., Ltd., Registration date: May 26, 2006) Republic of Korea Patent Registration No. 10-1155714 (Title of the invention: Cosmetic composition containing immature fruit or seed extract of birch tree, Applicant: AMOREPACIFIC, Registration date: June 05, 2012) Republic of Korea Patent No. 10-1648466 (Title of the invention: Cosmetic composition for skin improvement, Applicant: AMOREPACIFIC, Registration date: August 09, 2016) Republic of Korea Patent Registration No. 10-1147541 (Title of the invention: composition for external application for skin containing fermented woobangja, Applicant: Hanbul Cosmetics Co., Ltd., registration date: May 14, 2012) Korean Patent Laid-Open Patent No. 10-2017-0137462 (Title of the invention: composition for skin improvement containing complex herbal extract, Applicant: LG Household & Health Care, Publication date: December 13, 2017)

Seokwon Lim and 2 others, Understanding and Treatment of Skin Aging, Journal of the Skin Barrier Society, 4, pp71-80, 2002. Oriental Medicine, by Won Yong-hee, Baeksan Publishing House, 1999.10.24. Korean Food Dictionary, edited by Park Won-ki, Shinkwang Publishing Company, 1991.4.20. Dumans M et al., Journal of Drugs in Dematology, 6, s20-4, 2007. Fraizer, S. et al., Journal of Investigative Dermatology, 107(3), 404-11, 1996. Lee et al., J. Cosmetic Science, 49, 285-297, 1998. Nagase H and Woessner JF, Journal of Biological Chemistry, 274, 21491-21494, 1999. Tzellos TG et al., Experimental Dermatology, 18(12), 1028-1035, 2009.

An object of the present invention is to provide a cosmetic composition for improving skin wrinkles, improving elasticity, or moisturizing the skin, containing a mixed extract of A.

The present invention relates to a cosmetic composition for improving skin wrinkle, improving elasticity, or moisturizing the skin, containing a mixed extract of woobangja and bija ( Torreya grandis ).

The bija is characterized in that the seed and the outer skin of the seed are removed from the fruit and the remaining aril is used.

It is preferable that the friend and visa are mixed in a weight ratio of 5:1 to 15:1. More preferably, the right-hand character and the non-bija are mixed in a weight ratio of 10:1.

The mixed extract may be extracted using water, C1-C4 alcohol, or a mixed solution thereof as a solvent. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.

The mixed extract has an effect of increasing the expression of collagen type 1, and also has a function of increasing the expression of HAS-2 (Hyaluronic acid synthase-2).

The formulation of the cosmetic is preferably skin lotion, skin softener, skin toner, astringent, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap , cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

In addition, it is preferable that 1 to 30% by weight of the mixed extract of Ubangja and Bija is included in the cosmetic formulation, and it is best to include 2% by weight.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

The water-soluble vitamin may be any type of vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. and their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) are also water-soluble vitamins that can be used in the present invention. included in Water-soluble vitamins can be obtained by conventional methods, such as a microbial transformation method, a purification method from a microbial culture, an enzymatic method, or a chemical synthesis method.

The oil-soluble vitamins may be any as long as they can be formulated in the cosmetic composition, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol). and their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microbial culture, and an enzyme or chemical synthesis method.

The macromolecular peptide may be any as long as it can be blended into the cosmetic composition, and preferred examples include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. Polymeric peptides can be purified and obtained by conventional methods such as purification from a microbial culture solution, enzyme method or chemical synthesis method, or can be purified and used from natural products such as dermis of pigs or cattle, silk fiber of silkworms, etc.

The high molecular weight polysaccharide may be any compound as long as it can be blended into the cosmetic composition. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) is mentioned. For example, chondroitin sulfate or a salt thereof can be purified and used from mammals or fish.

The sphingolipid may be any as long as it can be blended into the cosmetic composition, and ceramide, phytosphingosine, sphingoglycolipid and the like are preferred. Sphingo lipids can be purified by conventional methods from mammals, fish, shellfish, yeast or plants, or can be obtained by chemical synthesis.

The seaweed extract may be any type as long as it can be formulated in the cosmetic composition, but preferably brown algae extract, red algae extract, green algae extract, etc., and calrageenan purified from these seaweed extracts, arginic acid, sodium alginate , potassium alginate, etc. are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purification from seaweed by a conventional method.

The cosmetic composition of the present invention may also contain other ingredients to be blended in a conventional cosmetic composition.

Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, a restrictive agent, purified water, etc. are mentioned.

Examples of the fats and oils include ester fats and oils, hydrocarbon oils, silicone oils, fluorine oils, animal fats, and vegetable oils.

As ester-based fats and oils, tri2-ethylhexanoate glyceryl, 2-ethylhexanoate cetyl, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic acid, capric acid) glyceryl, trimethylol propane tri-2-ethylhexanoate, trimethylol propane triisostearate, pentaelysitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostea laurate Lil, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearic acid isopropyl, 2-ethylhexanoate cetoster Aryl, 2-ethyl stearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, di(caprylic acid, capric acid) propylene glycol, dicaprylic acid propylene glycol, dicapric acid Neopentyl glycol phosphate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecyl, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , isononanoate octyl, neodecanoate hexyldecyl, neodecanoate octyldodecyl, isostearate isocetyl, isostearyl isostearate, isostearic acid octyldecyl, polyglycerol oleate, polyglycerol isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, dimalate Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl Ester type, such as hydroxy stearate isocetyl, 12-stealoyl hydroxy stearyl stearate, and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, petrolatum.

Examples of silicone-based oils and fats include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstealloxysiloxane copolymer, and alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Perfluoropolyether etc. are mentioned as fluorine-type fats and oils.

As animal or vegetable oils, avocado oil, almond oil, olive oil, sesame oil, rice bran oil, saffron oil, soybean oil, corn oil, rapeseed oil, passerine oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil , Cottonseed Oil, Palm Oil, Kukui Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Wormwood Colostrum, Marcus Damien Nut Oil, Meadow Oil, Egg Yolk Oil, Tallow Oil, Horse Oil, Mink Oil, Orange Raffy Oil, Jojoba Oil , animal or plant oils and fats such as candelabra wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As water-soluble low molecular weight moisturizers, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n=2 or more), polyglycerol B (polymerization degree n=2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Cholesterol, cholesterol ester, etc. are mentioned as a fat-soluble low molecular weight humectant.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, and dextrin. can

Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicocene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and high molecular weight silicone.

Examples of the emollient include long-chain acylglutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, etc. are mentioned as surfactant.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE/POP (polyoxyethylene/polyoxypropylene) copolymer, POE/POP alkyl ether, polyether-modified silicone, lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, and alkyl amide. phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkylether carboxylate, alkylsulfosuccinate salt, alkylsulfoacetate sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkylphosphate ester, etc. there is.

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, chloride Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salt of lanolin derivative, etc. are mentioned.

Examples of the amphoteric surfactant include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidamine type, etc. Amphoteric surfactant etc. are mentioned.

Examples of organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide. , inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, and organic pigments such as silk powder, cellulose, CI pigment yellow and CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate; alkyl phosphate metal salts such as sodium cetylate, zinc laurylate, and calcium laurylate; acylamino acid polyvalent metal salts such as calcium N-lauroyl-beta-alanine, zinc N-lauroyl-beta-alanine, and calcium N-lauroyl glycine; amidesulfonic acid polyvalent metal salts such as calcium N-lauroyl-taurine and calcium N-palmitoyl-taurine; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, a divinylbenzene-styrene copolymer, ethylene tetrafluoride, etc. are mentioned.

As UV absorbers, para-aminobenzoic acid, para-aminobenzoate ethyl, para-aminobenzoate amyl, para-aminobenzoate octyl, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butyl salicylate, homomentyl salicylate, benzyl cinnamate , para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexaneglyceryl, para-methoxycinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Cannic acid, ethyl urokanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone , tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Examples of disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc phyllithione, benzalkonium chloride, photosensitizer. So, No. 301, mononitroguaial sodium, undecyrenic acid, etc. are mentioned.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and ellisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmalic acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

As alcohol, higher alcohols, such as cetyl alcohol, are mentioned.

In addition, blending components that may be added other than this are not limited thereto, and any of the above components can be blended within a range that does not impair the purpose and effect of the present invention, but preferably 0.01 to 5 wt%, more preferably, based on the total weight may be blended in an amount of 0.01 - 3 wt%.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetics in addition to the extract as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carrier.

When the cosmetic composition formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. can be used

When the cosmetic composition formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon , propane/butane or propellants such as dimethyl ether.

When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol. , 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the cosmetic composition formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the cosmetic composition formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The present invention relates to a cosmetic composition for wrinkle improvement, elasticity improvement, or moisturizing of the skin comprising a mixed extract of woobangja and non-abrasicles as an active ingredient, wherein the extract increases the expression of collagen type 1 gene By inducing collagen biosynthesis, it is suggested that there is an effect of improving skin elasticity and wrinkles, and it shows that there is an excellent moisturizing function by increasing the gene expression of HAS-2 (Hyaluronic acid synthase-2).

1 shows the results for the collagen type I expression inducing effect of Examples 1 to 3 and Comparative Examples 1 to 22 extracts.
Figure 2 shows the results for the HAS-2 expression inducing effect of Examples 1 to 3 and Comparative Examples 1 to 22 extracts.
3 shows the results for the collagen type I expression inducing effect by concentration of the extract of Example 1.
Figure 4 shows the results for the HAS-2 expression induction effect for each concentration of the extract of Example 1.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to fully convey the spirit of the present invention to those skilled in the art so that this disclosure will be thorough and complete.

<Preparation Example 1. Preparation of each extract>

As a non-aril, the arachnid of Torreya grandis remaining after harvesting seeds was used for use as a nut snack in China. It was used as a comparison group. In addition, most effectively used in the birch tree are seeds or seed oil obtained by pressing the seeds, and an ethanol extract extracted with a solvent such as ethanol. As this, various raw materials of Torreya nucifera and Torreya grandis were used. Commonly referred to as a seed, but the commonly used right-wing root was also used as a comparison group.

Each sample was prepared in a dry state, immersed in a 10-fold weight 30% (w/w) aqueous ethanol solution, extracted at 60° C. for 5 hours, and then filtered with a 250 mesh filter cloth and filtered with a 0.3 μm filter. Then, the filtrate was concentrated under reduced pressure with a vacuum concentrator (EYELA, N-1000, Japan) to obtain an extract. In addition, non-Japanese seed oil was obtained by cold pressing using a cold press equipment. The extract of fenugreek used when preparing non-japanese seed oil as a mixture was prepared using only fenugreek alone in a 30% (w/w) aqueous ethanol solution.

The names of the test groups used in the present invention are designated as shown in Table 1 below, and the mixing ratio in Table 1 represents the weight ratio of each raw material sample.

designation test group control untreated group Example 1 10:1 Mixed Extracts of Astragalus and Torreya grandis Example 2 5:1 Mixed Extracts of Astragalus and Torreya grandis Example 3 15:1 Mixed Extracts of Astragalus and Torreya grandis Comparative Example 1 1:1 Mixed Extracts of Astragalus and Torreya grandis Comparative Example 2 20:1 Mixed Extracts of Astragalus and Torreya grandis Comparative Example 3 lavender extract Comparative Example 4 right root extract Comparative Example 5 Torreya grandis aril extract Comparative Example 6 Torreya nucifera aril extract Comparative Example 7 Torreya grandis seed oil Comparative Example 8 Torreya nucifera seed oil Comparative Example 9 Torreya grandis seed extract Comparative Example 10 Torreya nucifera seed extract Comparative Example 11 Torreya grandis leaf extract Comparative Example 12 Torreya nucifera leaf extract Comparative Example 13 Torreya grandis bark extract Comparative Example 14 Torreya nucifera bark extract Comparative Example 15 10:1 blended extract of algae and Torreya grandis seeds Comparative Example 16 10:1 Mixture of Allison Root Extract and Torreya grandis Seed Oil Comparative Example 17 10:1 mixed extract of algae and Torreya grandis leaves Comparative Example 18 10:1 blend extract of algae and Torreya grandis bark Comparative Example 19 10:1 mixed extracts of algae and Torreya nucifera seeds Comparative Example 20 A 10:1 mixture of lavender extract and Torreya nucifera seed oil Comparative Example 21 10:1 mixed extracts of algae and Torreya nucifera leaves Comparative Example 22 10:1 mixed extract of algae and Torreya nucifera bark

<Experimental Example 1: Cytotoxicity evaluation (MTT assay)>

In this experimental example, the cytotoxicity of the extracts of Examples 1 to 3 and Comparative Examples 1 to 22 was measured.

Human fibroblasts were inoculated in a 24-well plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum-free medium, and samples were treated by concentration and cultured for 24 hours. After incubation, the cells were reacted by replacing the 2.5 mg/ml MTT solution with a 10-fold diluted medium. Then, the supernatant was removed and DMSO was added to dissolve the MTT-formazan crystals produced and measure the absorbance at 570 nm with an ELISA Reader. did Cytotoxicity is expressed as a percentage of absorbance compared to the control, which is shown in Table 2 below.

As shown in Table 2 below, as a result of performing a cytotoxicity test to determine the sample concentration for efficacy evaluation of the extracts of Examples 1 to 3 and Comparative Examples 1 to 22, Examples 1 to 3 and Comparative Examples 1 to 22 extracts Silver showed no toxicity at a concentration of 250 μg/ml.

test group Cell viability (%) control 100.0 Example 1 96.1 Example 2 97.4 Example 3 98.8 Comparative Example 1 97.9 Comparative Example 2 96.5 Comparative Example 3 100.1 Comparative Example 4 98.8 Comparative Example 5 97.9 Comparative Example 6 101.1 Comparative Example 7 99.1 Comparative Example 8 102.3 Comparative Example 9 101.8 Comparative Example 10 103.9 Comparative Example 11 100.1 Comparative Example 12 100.9 Comparative Example 13 97.6 Comparative Example 14 96.2 Comparative Example 15 99.4 Comparative Example 16 100.6 Comparative Example 17 99.7 Comparative Example 18 102.5 Comparative Example 19 98.4 Comparative Example 20 99.0 Comparative Example 21 100.4 Comparative Example 22 98.4

<Experimental Example 2: Efficacy evaluation of skin elasticity - Collagen Type I expression investigation>

In this experimental example, the skin elasticity efficacy of the extracts of Examples 1 to 3 and Comparative Examples 1 to 22 was measured.

Fibroblast cells were inoculated on a cell plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum-free medium, and the sample was treated with the cells so that the final concentration was 250 μg/ml and cultured for 24 hours. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 3 and FIG. 1 below.

Test group (extract 250 μg/ml) Collagen type I gene expression rate (%) Control (untreated group) 100.0 Example 1 189.8 Example 2 176.4 Example 3 170.1 Comparative Example 1 101.4 Comparative Example 2 100.3 Comparative Example 3 133.0 Comparative Example 4 126.4 Comparative Example 5 132.7 Comparative Example 6 118.4 Comparative Example 7 127.8 Comparative Example 8 119.3 Comparative Example 9 122.1 Comparative Example 10 117.3 Comparative Example 11 112.8 Comparative Example 12 106.8 Comparative Example 13 114.6 Comparative Example 14 113.1 Comparative Example 15 119.8 Comparative Example 16 121.6 Comparative Example 17 106.3 Comparative Example 18 113.0 Comparative Example 19 117.3 Comparative Example 20 116.7 Comparative Example 21 107.3 Comparative Example 22 102.0

As a result of confirming the expression of collagen type I in each sample, the expression of collagen type I was significantly increased in the extracts of Examples 1 to 3, so that it can be understood that there is a synergistic effect of the skin elasticity function due to the mixed extraction.

<Experimental Example 3. Evaluation of skin moisturizing efficacy - Hyaluronic acid synthase-2, HAS-2 expression investigation>

In this experimental example, the skin moisturizing effect of the extracts of Examples 1 to 3 and Comparative Examples 1 to 22 was measured.

Fibroblast cells were inoculated into a 60 mm plate and cultured for 24 hours, then the cultured cell medium was replaced with a serum-free medium, and the sample was treated with the cells so that the final concentration was 250 μg/ml and cultured for 24 hours. Cells were collected with QIAzol™ Lysis Reagent (Qiagen) and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized and real-time PCR was performed. This is shown in Table 4 and Figure 2 below.

test group
(Extract 250 μg/ml) HAS-2
expression rate (%) Control (untreated group) 100.0 Example 1 176.9 Example 2 169.7 Example 3 160.0 Comparative Example 1 97.4 Comparative Example 2 103.6 Comparative Example 3 133.0 Comparative Example 4 131.4 Comparative Example 5 130.1 Comparative Example 6 117.4 Comparative Example 7 128.4 Comparative Example 8 120.3 Comparative Example 9 115.4 Comparative Example 10 107.8 Comparative Example 11 110.4 Comparative Example 12 113.4 Comparative Example 13 110.6 Comparative Example 14 106.4 Comparative Example 15 121.5 Comparative Example 16 122.4 Comparative Example 17 104.6 Comparative Example 18 109.3 Comparative Example 19 111.3 Comparative Example 20 110.0 Comparative Example 21 105.3 Comparative Example 22 108.4

As a result of confirming the expression of HAS-2 related to moisturizing in each sample, the value was significantly increased in the extracts of Examples 1 to 3, and it can be seen that there is a synergistic effect of the moisturizing function due to the mixed extraction.

<Experimental Example 4: Evaluation of skin elasticity and moisturizing efficacy - Collagen Type I, HAS-2 expression investigation>

In order to confirm the extract of Example 1, which has the best skin improvement effect, by concentration, an additional experiment was conducted. The experimental method is the same as the method described above, and the experimental results are shown in Tables 5 and 6 and 3 and 4 .

Test group (μg/ml) Collagen type I gene expression rate (%) Control (untreated group) 100.0 Example 1 25 126.4 50 137.6 100 159.8 250 181.0

Test group (μg/ml) HAS-2 gene expression rate (%) Control (untreated group) 100.0
Example 1 25 124.5 50 138.2 100 161.0 250 170.5

Example 1 As a result of confirming the expression of collagen type I and HAS-2 by treating the extract by concentration, it was confirmed that the expression was increased in a concentration-dependent manner.

<Cosmetic Preparation Example 1. Skin>

A skin was prepared as follows with the composition shown in Table 7 below, using as a raw material a 10:1 mixed extract of Ubangja and Torreya grandis arils dissolved to 2.0% (w/w). Glycerin, butylene glycol, propylene glycol, and a preservative were added to purified water in this order, stirred and dissolved to obtain a mixed solution. Polyoxyethylene hydrogenated castor oil was heated to about 60° C. to dissolve, and then a fragrance was added and dissolved, and then added to the mixture. Finally, a 2% aqueous solution of 10:1 mixed extracts of U.S. apricot and Torreya grandis arils, ethanol, triethanolamine, and a pigment were added, stirred sufficiently, and then aged.

Raw material weight% 2% (w/w) aqueous solution of 10:1 mixed extracts of Astragalus and Torreya grandis 2.0 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Polyoxyethylene fortified perm oil 1.0 ethanol 10.0 triethanolamine 0.1 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water To 100

<Cosmetics Manufacturing Example 2. Nutrient Lotion>

A nutrient lotion with the composition shown in Table 8 below was prepared by dissolving a 10:1 mixed extract of Ubangja and Torreya grandis agaric bark to 2.0% (w/w) as a raw material.

Propylene glycol, carboxypolymer, preservative, and purified water are mixed and stirred at 80 ~ 85°C and heated to between 80 and 85°C, put into the manufacturing unit, and then the emulsifier works, beeswax, polysorbate 60, sorbitan sesquioleate, liquid paraffin, sorbitan stearate , lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, and triethanolamine were dissolved by heating between 80 and 85° C. and then emulsified. After the emulsification is finished, use a stirrer to cool it down to 50°C, add flavoring , and after cooling to 45°C, add a dye, and at 35°C, 2% (w/w/ w) Aqueous solution was added, cooled to 25° C., and then aged.

Raw material weight% 2% (w/w) aqueous solution of 10:1 mixed extracts of Astragalus and Torreya grandis 2.0 beeswax 1.0 Polysorbate 60 1.5 Sorbitan Sesquiolate 0.5 liquid paraffin 10.0 Sorbitan Stearate 1.0 lipophilic monostearate glycerin 0.5 stearic acid 1.5 glyceryl stearate/PEG-400 stearate 1.0 propylene glycol 3.0 carboxy polymer 0.1 triethanolamine 0.2 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water To 100

<Cosmetic Preparation Example 3. Cream>

A nourishing cream was prepared as follows with the composition of Table 9 below by dissolving 10:1 mixed extracts of Ubangja and Torreya grandis arils to 2.0% (w/w) as a raw material. Carboxyvinyl polymer, butylene glycol, glycerin, and purified water are mixed and stirred at 80-85° C. and heated to between 80 and 85° C., put into the manufacturing unit, and then the emulsifier works, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate Rate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, liquid paraffin, squalane, caprylic / capric triglyceride by heating between 80 ~ 85 ℃ to dissolve Triethanolamine was added and stirred to put into the production unit and emulsified. After the emulsification was completed, the mixture was cooled to 35°C while stirring using a stirrer, and 2% (w/w) aqueous solution of a 10:1 mixed extract of U.S. apricot and Torreya grandis aril was added, cooled to 25°C, and then aged.

Raw material content (g) 2% (w/w) aqueous solution of 10:1 mixed extracts of Astragalus and Torreya grandis 2.0 stearic acid 2.0 cetyl alcohol 2.0 Glyceryl Monostearate 2.0 Polyoxyethylene sorbitan monostearate 0.5 Sorbitan sesquioleate 0.5 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 wax 1.0 liquid paraffin 4.0

Claims (5)

A cosmetic composition for wrinkle improvement, elasticity improvement or moisturizing of the skin, characterized in that it contains a mixed extract of Arctium lappa seed and Torreya grandis . According to claim 1,
The cosmetic composition for wrinkle improvement, elasticity improvement or moisturizing of the skin, characterized in that the non-bija uses the aril remaining after removing the seed and the outer skin of the seed from the fruit. According to claim 1,
The mixed extract is a cosmetic composition for wrinkle improvement, elasticity improvement or moisturizing of the skin, characterized in that it is extracted using water, C1-C4 alcohol or a mixed solution thereof as a solvent. According to claim 1,
The mixed extract is a cosmetic composition for improving wrinkles, improving elasticity or moisturizing the skin, characterized in that it increases the expression of collagen type 1. According to claim 1,
The mixed extract is a cosmetic composition for wrinkle improvement, elasticity improvement or moisturizing of the skin, characterized in that it increases the expression of HAS-2 (Hyaluronic acid synthase-2).

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