KR102070143B1 - Lactobacillus plantarum WiKim0060 having skin whitening and skin moisturizing activities and composition for comprising the same - Google Patents
Lactobacillus plantarum WiKim0060 having skin whitening and skin moisturizing activities and composition for comprising the same Download PDFInfo
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- KR102070143B1 KR102070143B1 KR1020170152395A KR20170152395A KR102070143B1 KR 102070143 B1 KR102070143 B1 KR 102070143B1 KR 1020170152395 A KR1020170152395 A KR 1020170152395A KR 20170152395 A KR20170152395 A KR 20170152395A KR 102070143 B1 KR102070143 B1 KR 102070143B1
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- wikim0060
- lactobacillus plantarum
- present
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- skin
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
본 발명은 김치로부터 분리된 신규한 락토바실러스 플란타럼 WiKim0060(Lactobacillus plantarum WiKim0060) 및 이를 포함하는 조성물에 관한 것이다. 본 발명에 따른 락토바실러스 플란타럼 WiKim0060은 멜라닌 및 홍반 생성 억제, 경피수분증발량 감소 활성을 갖는 프로바이오틱스로서, 사람 또는 동물의 피부 과색소성 질환의 예방 및 개선, 정장 등의 용도를 위해 다양하게 활용될 수 있다.The present invention is a novel Lactobacillus plantarum isolated from kimchi WiKim0060 ( Lactobacillus plantarum WiKim0060) and a composition comprising the same. Lactobacillus plantarum WiKim0060 according to the present invention is a probiotic having melanin and erythema production inhibition, transdermal moisture evaporation activity, and can be variously used for the prevention and improvement of skin hyperpigmented disease in humans or animals, suits, etc. Can be.
Description
본 발명은 신규한 락토바실러스 플란타럼 균주 및 이를 포함하는 조성물에 관한 것이다. The present invention relates to a novel Lactobacillus plantarum strain and a composition comprising the same.
태양 빛 중 자외선은 체내에서 비타민 D를 합성하고, 살균작용의 역할이 있으나, 피부염, 피부암, 일광 화상, 피부노화, 건조, 잔주름 등을 생기게도 한다. 자외선 중간 파장인 UVB(290-320nm)는 급성이나 만성적으로 노출되었을 경우, 심각한 피부 손상을 야기하는데, 피부 진피 내 구성성분이 콜라겐, 엘라스틴, 히알루론산을 파괴하여 피부의 탄력 손실을 유도하게 되어 그 결과 굵은 주름이 생성된다. 이러한 특징적인 변화들은 광 노화를 유도하며 피부 홍반이나 거칠기를 유도하는 것으로 알려져 있다. Ultraviolet rays of the sun light synthesize vitamin D in the body and play a role of bactericidal action, but also cause dermatitis, skin cancer, sunburn, skin aging, drying and fine lines. UVB (290-320nm), a medium wavelength of ultraviolet radiation, causes severe skin damage when exposed to acute or chronic exposure. The components in the dermis of the skin destroy collagen, elastin and hyaluronic acid, leading to loss of elasticity of the skin. The result is a thick crease. These characteristic changes induce light aging and are known to induce skin erythema or roughness.
또한, 광 노화로 인해 흑화 증상이 발생되는데, 이는 피부색을 결정하는 가장 중요한 색소인 멜라닌에 의한다. 멜라닌은 기저층에 존재하는 멜라노사이트 내의 멜라노좀에서 합성되며, 피부 세포 내의 멜라닌 양에 영향을 받게 된다. 이러한 광 노화에 의한 피부손상을 예방하기 위한 노력으로 주름, 보습 및 흑화 개선 화장료로써 유산균, 효모와 같은 미생물을 화장품 산업에 사용하는 예도 증가되고 있다. In addition, light aging causes blackening symptoms, which is due to melanin, the most important pigment that determines skin color. Melanin is synthesized in melanosomes in melanocytes present in the basal layer and is affected by the amount of melanin in skin cells. In an effort to prevent skin damage due to light aging, examples of using microorganisms such as lactic acid bacteria and yeast as cosmetics for improving wrinkles, moisturizing and blackening are increasing.
유산균은 인체 내에서 유익한 역할을 하는 미생물로서, 특히, 위 장관 면역 증강의 생리활성을 가진다고 알려져 있으며, 최근에는 경구섭취 하였을 때 피부세포의 면역조절에도 영향을 끼친다는 보고들이 있다. 하지만 유산균의 발효액, 배양액이 아닌 유산균 자체의 생균 섭취와 관련된 피부미백 및 보습 개선 효과에 대해서는 아직 알려진 바가 없다.Lactobacillus is a microorganism that plays a beneficial role in the human body, in particular, it is known to have a physiological activity of gastrointestinal immunity enhancement, and recently there are reports that it affects the immune regulation of skin cells when orally ingested. However, the effect of improving skin whitening and moisturizing related to the intake of live bacteria of lactic acid bacteria itself, not the culture of lactic acid bacteria, is not known.
본 발명은 피부미백 및 보습개선 활성이 우수한 신규한 락토바실러스 속 유산균을 제공하고자 한다.The present invention is to provide a novel Lactobacillus genus lactic acid bacteria excellent in skin whitening and moisturizing improvement activity.
이에, 본 발명자들은 전통발효식품으로부터 프로바이오틱스로서 우수한 효과를 나타내면서 피부미백 및 보습개선 효과를 나타내는 유산균 균주를 찾고자 노력한 결과, 신규한 락토바실러스 속 유산균 균주인 락토바실러스 플란타럼 WiKim0060(Lactobacillus plantarum WiKim0060)을 분리, 동정하여 본 발명을 완성하게 되었다.Accordingly, the present inventors have tried to find a lactic acid bacteria strain exhibiting excellent skin whitening and moisturizing effect while showing an excellent effect as a probiotic from traditional fermented foods, Lactobacillus plantarum WiKim0060 ( Lactobacillus plantarum WiKim0060), a novel lactobacillus lactic acid bacteria strain The present invention has been completed by separation and identification.
본 발명에 따른 락토바실러스 플란타럼 WiKim0060(Lactobacillus plantarum WiKim0060)은 김치 유래의 락토바실러스 플란타럼 신규 균주이다. 비록 본 발명에서의 락토바실러스 플란타럼 WiKim0060을 김치에서 분리, 동정하기는 했으나, 이의 입수 경로가 이에 한정되는 것은 아니다. Lactobacillus plantarum WiKim0060 ( Lactobacillus plantarum WiKim0060) according to the present invention is a novel strain of Lactobacillus plantarum derived from kimchi. Although the Lactobacillus plantarum WiKim0060 in the present invention was isolated and identified in kimchi, the access route thereof is not limited thereto.
본 발명에의 실시예를 통해 분리된 전통발효식품으로부터 프로바이오틱스로서 우수한 효과를 나타내는 유산균 균주는 미생물의 동정 및 분류를 위한 16S rDNA 염기서열 분석 결과, SEQ ID NO: 1의 핵산서열을 갖는 것으로 나타났다. Lactobacillus strains showing excellent effects as probiotics from the traditional fermented foods isolated through the example of the present invention was found to have a nucleic acid sequence of SEQ ID NO: 1 as a result of 16S rDNA sequencing for identification and classification of microorganisms.
따라서, SEQ ID NO: 1의 16S rDNA 염기서열을 갖는 본 발명의 미생물을 락토바실러스 플란타럼 WiKim0060(Lactobacillus plantarum WiKim0060)로 명명하였으며, 한국미생물보존센터에 2017년 10월 18일자로 기탁하였다(수탁번호 KCCM12132P).Thus, the microorganism of the present invention having the 16S rDNA sequence of SEQ ID NO: 1 was isolated from Lactobacillus plantarum WiKim0060 ( Lactobacillus plantarum WiKim0060) and was deposited with the Korea Microorganism Conservation Center on October 18, 2017 (Accession No. KCCM12132P).
본 발명의 락토바실러스 플란타럼 WiKim0060은 그람양성균이고 호기적 조건과 혐기적 조건에서 모두 성장이 가능한 통성 혐기성(facultative anaerobe)이며, 포자를 형성하지 않고 운동성이 없으며 세포는 간균의 형태를 취하고 있다. The Lactobacillus plantarum WiKim0060 of the present invention is a gram-positive bacterium, a facultative anaerobe capable of growing in both aerobic and anaerobic conditions, does not form spores, has no mobility, and the cells have a form of bacilli.
본 발명의 락토바실러스 플란타럼 WiKim0060은 프로바이오틱스로서, 유산균의 일반적인 정장 효과 및 면역 증강 효과를 갖는다. 락토바실러스 속의 유산균이 정장 효과 면역 증강 효과를 갖는다는 것은 잘 알려져 있는 사실이다.The Lactobacillus plantarum WiKim0060 of the present invention is a probiotic and has a general intestinal effect and an immune enhancing effect of lactic acid bacteria. It is well known that lactic acid bacteria of the genus Lactobacillus have a formal effect immune enhancing effect.
본 발명에 있어서, '프로바이오틱스(probiotics)'는 사람을 포함한 동물의 위장관 내에서 숙주의 장내 미생물 환경을 개선하여 숙주의 건강에 유익한 영향을 주는 살아있는 미생물'이라는 의미로 이해된다. 프로바이오틱스는 프로바이오틱 활성을 갖는 살아있는 미생물로 단일 또는 복합균주 형태로 사람이나 동물에 건조된 세포 형태나 발효산물 형태로 급여될 경우, 숙주의 장내 균총에 유익한 영향을 미칠 수 있다. In the present invention, 'probiotics' is understood to mean a living microorganism that has a beneficial effect on the health of the host by improving the intestinal microbial environment of the host in the gastrointestinal tract of animals including humans. Probiotics are living microorganisms with probiotic activity that can have a beneficial effect on the intestinal flora of the host when fed in the form of dried cells or fermented products to humans or animals in the form of single or complex strains.
하기 실시예에서는, 본 발명의 락토바실러스 플란타럼 WiKim0060이 UVB 조사 무모 마우스에서 광 손상에 의해 유도되는 멜라닌 및 홍반을 억제하고, 경피수분증발량을 감소시킴으로써 락토바실러스 플란타럼 WiKim0060 균주가 피부미백 및 보습개선 효과를 나타내는 것을 확인하였다. 따라서, 본 발명에 따른 락토바실러스 플란타럼 WiKim0060은 프로바이오틱스로서 사람 또는 동물의 정장, 면역강화, 피부미백 및 보습개선의 용도를 위해 다양하게 활용될 수 있다. In the following examples, the Lactobacillus plantarum WiKim0060 of the present invention inhibits melanin and erythema induced by light damage in UVB irradiated hairless mice, and reduces the transdermal moisture evaporation, thereby reducing the skin whitening and It was confirmed that the moisturizing improvement effect. Therefore, the Lactobacillus plantarum WiKim0060 according to the present invention can be variously used as a probiotic for the use of human or animal suits, immune strengthening, skin whitening and moisturizing improvement.
이에, 본 발명의 한 구체예에서는 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물을 포함하는 프로바이오틱스 조성물을 제공한다. Thus, one embodiment of the present invention provides a probiotic composition comprising Lactobacillus plantarum WiKim0060 or a culture thereof.
본 발명에 따른 조성물에 포함되는 락토바실러스 플란타럼 WiKim0060은 생균체 또는 사균체로서 존재할 수 있으며, 또한 건조 또는 동결건조된 형태로 존재할 수도 있다. 다양한 조성물 내에 포함시키기 적합한 유산균의 형태 및 제제화 방법은 당업자에게 잘 알려져 있다. The Lactobacillus plantarum WiKim0060 included in the composition according to the present invention may exist as live or dead microorganisms and may also exist in a dried or lyophilized form. Forms and formulation methods of lactic acid bacteria suitable for inclusion in various compositions are well known to those skilled in the art.
한 구체예에서, 상기 조성물은 생균으로 존재하는 락토바실러스 플란타럼 WiKim0060 균주를 포함하는 경구 투여용 조성물일 수 있다.In one embodiment, the composition may be a composition for oral administration comprising the Lactobacillus plantarum WiKim0060 strain present in live bacteria.
다른 한 구체예에서, 상기 조성물은 락토바실러스 플란타럼 WiKim0060 균주 또는 이의 배양물을 포함하는 피부 도포용 조성물일 수 있다.In another embodiment, the composition may be a composition for skin application comprising the Lactobacillus plantarum WiKim0060 strain or a culture thereof.
또한, 본 발명의 한 구체예에서는 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물을 포함하는 정장제 조성물을 제공한다. 본 발명에 따른 정장제 조성물은 사람을 포함한 동물의 위장 질환의 예방, 치료, 개선에 이용될 수 있으며, 바람직하게는 상기 동물은 소, 말, 돼지와 같은 가축을 포함한다. 상기 '위장 질환'으로는 위장 위해 세균감염 및 염증성 장 질환을 모두 포함하며, 예를 들어 병원성 미생물(대장균, 살모넬라, 클로스트리디움 등)에 의한 감염성 설사, 위장염증, 염증성 장 질환, 신경성 장염 증후군, 소장 미생물 과성장증, 장 급이성 설사 등을 포함하지만, 이에 한정되는 것은 아니다. In addition, one embodiment of the present invention provides a formal formulation comprising Lactobacillus plantarum WiKim0060 or a culture thereof. The formal formulation according to the present invention can be used for the prevention, treatment and amelioration of gastrointestinal diseases in animals including humans. Preferably, the animals include livestock such as cattle, horses and pigs. The 'gastrointestinal disease' includes both gastrointestinal harmful bacterial infection and inflammatory bowel disease, for example, infectious diarrhea caused by pathogenic microorganisms (E. coli, Salmonella, Clostridium, etc.), gastrointestinal inflammation, inflammatory bowel disease, neuro enteritis syndrome , Small intestine microbial hypergrowth, enterotropic diarrhea, and the like.
본 발명에 따른 정장제 조성물은 경구로 투여하는 것이 바람직하다. 투여량은 위장 질환의 종류, 질환의 정도, 연령, 성별, 인종, 치료 또는 예방 목적 등에 따라 달라질 수 있으나, 일반적으로 성인을 기준으로 하루에 1천만 마리에서 1,000억마리를 투여할 수 있다. The formal formulation according to the invention is preferably administered orally. The dosage may vary depending on the type of gastrointestinal disease, the severity of the disease, age, sex, race, therapeutic or prophylactic purpose, etc., but in general, 10 to 100 billion animals may be administered per adult.
본 발명은 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물을 포함하는 면역 증강용 조성물을 제공한다. 락토바실러스 속의 유산균이 정장 효과 면역 증강 효과를 갖는다는 것은 잘 알려져 있는 사실이다. The present invention provides a composition for enhancing immunity comprising Lactobacillus plantarum WiKim0060 or a culture thereof. It is well known that lactic acid bacteria of the genus Lactobacillus have a formal effect immune enhancing effect.
본 발명은 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물을 포함하는 피부미백 및 보습개선용 조성물을 제공한다. 또한, 본 발명은 과색소 침착(hypopigmentation)의 예방, 개선 및 치료용 조성물을 제공한다. 상기 과색소 침착은 자외선 조사에 의한 멜라닌 과잉 합성 및 축적에 따른 색소 침착으로, 예컨대 기미, 주근깨 또는 흑자를 포함할 수 있다.The present invention provides a composition for skin whitening and moisturizing, including Lactobacillus plantarum WiKim0060 or a culture thereof. The present invention also provides compositions for the prevention, improvement and treatment of hyperpigmentation. The hyperpigmentation may be pigmentation due to melanin oversynthesis and accumulation by ultraviolet irradiation, such as blemishes, freckles or surpluses.
본 발명에 있어서, “피부 미백”은 자외선 조사에 따른 색소 침착이나 홍반 및 멜라닌의 생성을 억제, 감소 또는 개선시키는 효과를 의미한다.In the present invention, "skin whitening" means the effect of inhibiting, reducing or improving pigmentation or the production of erythema and melanin by ultraviolet irradiation.
본 발명에 있어서, “보습 개선”은 경피의 수분 증발량이 감소되는 효과를 의미한다.In the present invention, "improving moisturizing" means an effect of reducing the amount of water evaporation of the transdermal.
본 발명에 따른 락토바실러스 플란타럼 WiKim0060은 이러한 유리한 작용으로 인해 의약, 건강기능식품, 식품, 사료, 사료 첨가제, 유산균 스타터 또는 화장료 내에 포함될 수 있다. Lactobacillus plantarum WiKim0060 according to the present invention may be included in medicine, nutraceuticals, food, feed, feed additives, lactobacillus starters or cosmetics due to this advantageous action.
한 구체예에서, 본 발명의 조성물이 약제학적 조성물로 활용될 경우, 본 발명의 약제학적 조성물은 상기 유효성분 이외에 약제학적으로 적합하고 생리학적으로 허용되는 보조제를 사용하여 제조될 수 있으며, 상기 보조제로는 부형제, 붕해제, 감미제, 결합제, 피복제, 팽창제, 윤활제, 활택제 또는 향미제 등을 사용할 수 있다.In one embodiment, when the composition of the present invention is utilized as a pharmaceutical composition, the pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, the adjuvant Furnace may be used excipients, disintegrants, sweeteners, binders, coatings, expanding agents, lubricants, lubricants or flavoring agents.
상기 약제학적 조성물은 투여를 위해서 상기 기재한 유효성분 이외에 추가로 약제학적으로 허용가능한 담체를 1종 이상 포함하여 약제학적 조성물로 바람직하게 제제화할 수 있다.The pharmaceutical composition may be preferably formulated into a pharmaceutical composition including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredient for administration.
예를 들어, 정제 또는 캡슐제의 형태로의 제제화를 위해, 유효성분은 에탄올, 글리세롤, 물 등과 같은 경구, 무독성의 약제학적으로 허용가능한 불활성 담체와 결합될 수 있다. 또한, 원하거나 필요한 경우, 적합한 결합제, 윤활제, 붕해제 및 발색제 또한 혼합물로 포함될 수 있다. 적합한 결합제는 이에 제한되는 것은 아니나, 녹말, 젤라틴, 글루코스 또는 베타-락토오스와 같은 천연당, 옥수수감미제, 아카시아, 트래커캔스 또는 소듐올레이트와 같은 천연 및 합성검, 소듐 스테아레이트, 마그네슘 스테아레이트, 소듐 벤조에이트, 소듐아세테이트, 소듐 클로라이드등을 포함한다. 붕해제는 이에 제한되는 것은 아니나, 녹말, 메틸셀룰로스, 아가, 벤토니트, 잔탄검 등을 포함한다. 액상 용액으로 제제화되는 조성물에 있어서 허용가능한 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충식염수, 알부민 주사 용액, 덱스트로즈 용액, 말토덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, nontoxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include, but are not limited to, natural and synthetic gums such as natural sugars such as starch, gelatin, glucose or beta-lactose, corn sweeteners, acacia, trackercance or sodium oleate, sodium stearate, magnesium stearate, sodium benzo 8, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methylcellulose, agar, bentonite, xanthan gum, and the like. Acceptable pharmaceutical carriers in compositions formulated as liquid solutions are sterile and physiologically compatible, including saline, sterile water, Ringer's solution, buffered saline, albumin injection solutions, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.
나아가 해당 분야의 적절한 방법으로 Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA에 개시되어 있는 방법을 이용하여 각 질환에 따라 또는 성분에 따라 바람직하게 제제화할 수 있다.Furthermore, the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be formulated according to each disease or component according to the appropriate method in the art.
다른 구체예에서, 본 발명은 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물을 유효성분으로 포함하는 피부미백 및 보습개선용 식품일 수 있다. 상기 식품 조성물은 건강기능식품 또는 음료, 바 등의 형태를 포함할 수 있다. In another embodiment, the present invention may be a skin whitening and moisturizing improvement food comprising Lactobacillus plantarum WiKim0060 or its culture as an active ingredient. The food composition may include the form of health food or beverages, bars and the like.
본 발명에 있어서, 상기 균주를 유효성분으로 포함하는 식품 조성물은 발효유 등의 음료를 포함할 수 있다. 이에, 본 발명은 락토바실러스 플란타럼 WiKim0060 또는 이의 배양물로 이루어지는 발효용 유산균 스타터를 제공한다.In the present invention, the food composition comprising the strain as an active ingredient may include a beverage such as fermented milk. Accordingly, the present invention provides a lactobacillus starter for fermentation consisting of Lactobacillus plantarum WiKim0060 or a culture thereof.
본 발명에 따른 식품 조성물은 상기 약제학적 조성물과 동일한 방식으로 제제화되어 기능성 식품으로 이용하거나, 각종 식품에 첨가할 수 있다. 본 발명의 조성물을 첨가할 수 있는 식품으로는 예를 들어, 음료류, 비타민복합제, 건강보조식품류 등이 있다.The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition, used as a functional food, or added to various foods. Examples of the food to which the composition of the present invention may be added include beverages, vitamin complexes, and health supplements.
본 발명의 식품 조성물은 식품 제조시에 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함한다. 상술한 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아추출물 (예를 들어 레바우디오시드A, 글리시르히진 등]) 및 합성향미제(사카린, 아스파르탐 등)를 사용할 수 있다. 예컨대, 본 발명의 식품 조성물이 드링크제와 음료류로 제조되는 경우에는 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 및 각 종 식물 추출액 등을 추가로 포함시킬 수 있다.The food composition of the present invention may include ingredients that are commonly added during food production, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. Examples of the above carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As flavoring agents, natural flavoring agents (tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared with a drink and a beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be further included.
상기 본 발명에 따른 조성물은 사료첨가제 또는 사료로서 이용될 수 있다.The composition according to the present invention can be used as a feed additive or feed.
사료 첨가제로서 이용될 경우, 상기 조성물은 20 내지 90% 고농축액이거나 분말 또는 과립 형태로 제조될 수 있다. 상기 사료첨가제는 구연산, 후말산, 아디픽산, 젖산, 사과산등의 유기산이나 인산 나트륨, 인산 칼륨, 산성 피로인산염, 폴리인산염(중합인산염) 등의 인산염이나, 폴리페놀, 카테킨, 알파-토코페롤, 로즈마리 추출물, 비타민 C, 녹차 추출물, 감초 추출물, 키토산, 탄닌산, 피틴산 등의 천연 항산화제 중 어느 하나 또는 하나 이상을 추가로 포함할 수 있다. 사료로서 이용될 경우, 상기 조성물은 통상의 사료 형태로 제제화될 수 있으며, 통상의 사료성분을 함께 포함할 수 있다.When used as a feed additive, the composition may be 20 to 90% high concentrate or prepared in powder or granule form. The feed additives include organic acids such as citric acid, fumaric acid, adipic acid, lactic acid, malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate and polyphosphate (polyphosphate), polyphenols, catechins, alpha-tocopherols, and rosemary. Extracts, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, phytic acid, such as any one or more of the natural antioxidant may be further included. When used as a feed, the composition may be formulated in a conventional feed form and may include common feed ingredients.
상기 사료첨가제 및 사료는 곡물, 예를 들면 분쇄 또는 파쇄된 밀, 귀리, 보리, 옥수수 및 쌀; 식물성 단백질 사료, 예를 들면 평지, 콩, 및 해바라기를 주성분으로 하는 사료; 동물성 단백질 사료, 예를 들면 혈분, 육분, 골분 및 생선분; 당분 및 유제품, 예를 들면 각종 분유 및 유장 분말로 이루어지는 건조성분 등을 더 포함할 수 있으며, 이외에도 영양보충제, 소화 및 흡수향상제, 성장촉진제 등을 더 포함할 수 있다.The feed additives and feeds include cereals such as milled or crushed wheat, oats, barley, corn and rice; Vegetable protein feed, such as feed consisting mainly of rapeseed, soybean, and sunflower; Animal protein feeds such as blood meal, meat meal, bone meal and fish meal; Sugars and dairy products, for example, may further include a dry ingredient consisting of various powdered milk and whey powder, and may further include nutritional supplements, digestive and absorption enhancers, growth promoters and the like.
상기 사료첨가제는 동물에게 단독으로 투여하거나 식용 담체 중에서 다른 사료첨가제와 조합하여 투여할 수도 있다. 또한, 상기 사료첨가제는 탑드레싱으로서 또는 이들을 동물사료에 직접 혼합하거나 또는 사료와 별도의 경구제형으로 용이하게 동물에게 투여할 수 있다. 상기 사료첨가제를 동물사료와 별도로 투여할 경우, 당해 기술분야에 잘 알려진 바와 같이 약제학적으로 허용가능한 식용 담체와 조합하여, 즉시 방출 또는 서방성 제형으로 제조할 수 있다. 이러한 식용 담체는 고체 또는 액체, 예를 들어 옥수수전분, 락토오스, 수크로오스, 콩플레이크, 땅콩유, 올리브유, 참깨유 및 프로필렌글리콜일 수 있다. 고체 담체가 사용될 경우, 사료첨가제는 정제, 캡슐제, 산제, 트로키제 또는 함당정제 또는 미분산성 형태의 탑 드레싱일 수 있다. 액체 담체가 사용될 경우, 사료첨가제는 젤라틴 연질 캡슐제, 또는 시럽제나 현탁액, 에멀젼제, 또는 용액제의 제형일 수 있다. The feed additive may be administered alone or in combination with other feed additives in an edible carrier. In addition, the feed additive may be easily administered to the animal as a top dressing or mixed directly with the animal feed or in an oral formulation separate from the feed. When the feed additive is administered separately from the animal feed, it can be prepared in immediate release or sustained release formulation in combination with a pharmaceutically acceptable edible carrier, as is well known in the art. Such edible carriers may be solid or liquid, for example corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol. If a solid carrier is used, the feed additive may be a tablet, capsule, powder, troche or sugar-containing tablet or top dressing in undispersible form. If a liquid carrier is used, the feed additive may be a gelatin soft capsule or a formulation of syrup or suspension, emulsion, or solution.
또한, 상기 사료첨가제 및 사료는 보조제, 예를 들어 보존제, 안정화제, 습윤제 또는 유화제, 용액촉진제 등을 함유할 수 있다. 상기 사료첨가제는 침주, 분무 또는 혼합하여 동물의 사료에 첨가하여 이용될 수 있다.In addition, the feed additives and feed may contain auxiliaries, such as preservatives, stabilizers, wetting agents or emulsifiers, solution accelerators and the like. The feed additive may be used in addition to the feed of the animal by acupuncture, spraying or mixing.
본 발명의 사료 또는 사료첨가제는 포유류, 가금 및 어류를 포함하는 다수의 동물식이에 적용할 수 있다.The feed or feed additive of the present invention can be applied to a number of animal diets, including mammals, poultry and fish.
상기 포유류로서 돼지, 소, 양, 염소, 실험용 설치동물, 및 실험용 설치동물뿐만 아니라, 애완동물(예: 개, 고양이) 등에게 사용할 수 있으며, 상기 가금류로서 닭, 칠면조, 오리, 거위, 꿩, 및 메추라기 등에도 사용할 수 있고, 상기 어류로서 송어 등에 이용될 수 있으나, 이에 한정되는 것은 아니다.The mammals can be used not only for pigs, cattle, sheep, goats, experimental rodents, and experimental rodents, but also for pets (eg, dogs, cats), and the like as chicken, turkey, duck, goose, pheasant, And can also be used for quail, etc., may be used in trout, etc. as the fish, but is not limited thereto.
본 발명에 따른 조성물에 포함되는 락토바실러스 플란타럼 WiKim0060 균주의 양은 1회를 기준으로 약 106 내지 1012 cfu/g일 수 있으며, 예컨대 107 내지 1011 cfu/g, 108 내지 1010 cfu/g일 수 있다. 균주를 투여할 경우에는 생균 상태로 투여하는 것이 바람직하며, 섭취 전에 사멸시키거나 감쇄(attenuation) 상태로 투여할 수 있다. 또한, 배양 상등액 등을 사용하여 제조할 경우에는 열처리 과정을 통한 멸균화 과정을 추가적으로 거칠 수 있다. 최소의 효능을 가지는데 필요한 균주량 및 일일 섭취 정도는 섭취자의 신체 또는 건강상태에 따라 달라질 수 있으나, 일반적으로 약 106 내지 1012 cfu/g일 수 있으며, 예컨대 107 내지 1011 cfu/g, 108 내지 1010 cfu/g일 수 있다.The amount of Lactobacillus plantarum WiKim0060 strain included in the composition according to the present invention may be about 10 6 to 10 12 cfu / g based on one time, such as 10 7 to 10 11 cfu / g, 10 8 to 10 10 cfu / g. In the case of administering the strain, it is preferable to administer in a viable state, and it may be administered before ingestion or in attenuated state. In addition, in the case of using the culture supernatant, etc., the sterilization process through heat treatment may be additionally performed. The amount of strain and daily intake required to have minimal efficacy may vary depending on the body or health of the ingestor, but may generally be about 10 6 to 10 12 cfu / g, such as 10 7 to 10 11 cfu / g , 10 8 to 10 10 cfu / g.
상기 본 발명에 따른 조성물은 피부 미백 및 보습 개선용 화장료로서 이용될 수 있다.The composition according to the present invention can be used as a cosmetic for improving skin whitening and moisturizing.
상기 피부미백 및 보습개선은 위에서 기술한 모든 내용을 그대로 적용시킬 수 있다.The skin whitening and moisturizing improvement can be applied as described above all.
본 발명의 화장료 조성물은 화장료 제조시 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 담체 등을 포함할 수 있다. 상기 화장료 조성물은 당업계에서 통상적으로 제조되는 제형으로 제조될 수 있으며, 예컨대, 용액, 현탁액, 유탁액, 페이스트, 젤, 크림, 로션, 파우더, 비누, 오일, 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention may include components commonly added when preparing cosmetics, and may include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, carriers, and the like. have. The cosmetic composition may be prepared in a formulation commonly prepared in the art, for example, may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, oil, foundation, spray, and the like. However, it is not limited thereto.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 젤인 경우, 담체 성분으로 동물성 오일, 식물성 오일, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다. 본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다. 본 발명의 화장료 조성물 제형이 용액 또는 유탁액인 경우 담체 성분으로 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다. 본 발명의 화장료 조성물의 제형이 현탁액인 경우 담체 성분으로 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier component may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, or the like. This can be used. When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon. Propellant, such as propane / butane or dimethyl ether. When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan. When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
본 발명의 이점 및 특징, 그리고 그것들을 달성하는 방법은 상세하게 후술되어있는 실시예들을 참조하면 명확해질 것이다. 그러나 본 발명은 이하에서 개시되는 실시예들에 한정되는 것이 아니라 서로 다른 다양한 형태로 구현될 것이며, 단지 본 실시예들은 본 발명의 개시가 완전하도록 하고, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 발명의 범주를 완전하게 알려주기 위해 제공되는 것이며, 본 발명은 청구항의 범주에 의해 정의될 뿐이다.Advantages and features of the present invention and methods of achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms, and only the present embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the art to which the present invention pertains. It is provided to fully convey the scope of the invention to those skilled in the art, and the present invention is defined only by the scope of the claims.
본 발명에 따른 락토바실러스 플란타럼 WiKim0060은 멜라닌 및 홍반 생성 억제, 경피수분증발량 감소 활성을 나타내므로, 프로바이오틱스로서 사람 또는 동물의 정장, 면역강화, 위장 질환, 피부미백 및 보습개선의 용도를 위해 다양하게 활용될 수 있다. 나아가, 발효용 스타터로서 유용하게 사용할 수 있다. Since the Lactobacillus plantarum WiKim0060 according to the present invention exhibits melanin and erythema production inhibition and transdermal moisture evaporation activity, it is various for probiotics for the use of human or animal suits, immune strengthening, gastrointestinal diseases, skin whitening and moisturizing improvement. Can be utilized. Furthermore, it can be usefully used as a starter for fermentation.
도 1은 본 발명에 따른 WiKim0060 균주 처리에 따른 멜라닌 및 홍반 생성 억제 효과를 육안으로 관찰한 사진이다.
도 2는 본 발명에 따른 WiKim0060 균주 처리에 따른 멜라닌 정도를 측정한 그래프이다.
도 3은 본 발명에 따른 WiKim0060 균주 처리에 따른 홍반 정도를 측정한 그래프이다.
도 4는 본 발명에 따른 WiKim0060 균주 처리에 따른 경피수분증발량을 측정한 그래프이다.1 is a photograph of visual observation of the melanin and erythema production inhibitory effect of the WiKim0060 strain treatment according to the present invention.
Figure 2 is a graph measuring the melanin degree according to the WiKim0060 strain treatment according to the present invention.
Figure 3 is a graph measuring the erythema degree according to the WiKim0060 strain treatment according to the present invention.
Figure 4 is a graph measuring the transdermal moisture evaporation according to the WiKim0060 strain treatment according to the present invention.
이하, 본 발명을 실시예를 통해 상세히 설명한다. 하기 실시예는 본 발명을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail through examples. The following examples are merely illustrative of the present invention, and the scope of the present invention is not limited to the following examples.
[실시예]EXAMPLE
실시예 1: 균주의 분리 및 동정Example 1 Isolation and Identification of Strains
김치 추출물의 원액을 MRS 배지에 도말하여 얻은 균단일집락을 루프로 수거하여 MRS broth에 배양하였다. DNA 추출은 QIAamp DNA Mini Kit(QIAgen, Germany)를 사용하여 추출하였다. 추출된 DNA는 1.5% 아가로스 겔을 이용하여 확인하였으며, 16S rDNA gene을 증폭하기 위하여 추출된 genomic DNA를 주형으로 하여 PCR을 진행하였고, PCR 조건은 denaturation 95℃ 1분, annealing 45℃ 1분, extension 72℃ 1분 30초로 30 사이클을 수행하였다. 얻어진 PCR 산물은 마크로젠(Seoul, Korea)에 의뢰하여 서열을 분석하였다. 세균의 동정은 16S rDNA 서열을 National Center for Biotechnology Information(NCBI, www.ncbi.nlm.nih.gov)의 Basic Local Alignment Search Tool (BLAST) 검색엔진의 유사도 분석을 통해 수행하였다. Homogeneous colonies obtained by spreading the stock solution of kimchi extract on MRS medium were collected in a loop and incubated in MRS broth. DNA extraction was extracted using the QIAamp DNA Mini Kit (QIAgen, Germany). The extracted DNA was identified using 1.5% agarose gel. PCR was performed using genomic DNA extracted as a template to amplify the 16S rDNA gene. PCR conditions were 1 minute denaturation at 95 ° C, 1 minute at annealing at 45 ° C, 30 cycles were performed at extension 72 ° C. 1 minute 30 seconds. The obtained PCR product was commissioned by Macrogen (Seoul, Korea) to analyze the sequence. Bacterial identification was performed through similarity analysis of the 16S rDNA sequence of the Basic Local Alignment Search Tool (BLAST) search engine of the National Center for Biotechnology Information (NCBI, www.ncbi.nlm.nih.gov).
본 발명의 실시예를 통해 분리된 균주는 미생물의 동정을 위한 16S rDNA 염기서열 분석 결과, SEQ ID NO: 1의 핵산서열을 갖는 것으로 나타났다. The strain isolated through the embodiment of the present invention was found to have a nucleic acid sequence of SEQ ID NO: 1, 16S rDNA sequence analysis for the identification of microorganisms.
이에, 본 발명의 미생물을 락토바실러스 플란타럼 WiKim0060 (Lactobacillus plantarum WiKim0060)으로 명명하였으며, 한국미생물보존센터에 2017년 10월 18일자로 기탁하였다(KCCM12132P).Thus, the microorganism of the present invention was named Lactobacillus plantarum WiKim0060 ( Lactobacillus plantarum WiKim0060), and was deposited on October 18, 2017 to the Korea Microorganism Conservation Center (KCCM12132P).
실시예Example 2: 2: 락토바실러스Lactobacillus 플란타럼Plantarum WiKim0060의Of WiKim0060 피부미백Skin whitening 및 보습개선 효능 확인 And moisturizing improvement efficacy
상기 실시예 1에서 분리한 락토바실러스 플란타럼 WiKim0060 균주는 MRS 배지에서 30℃, 24시간 동안 배양하고, 배양된 균체를 8,000rpm에서 5분 동안 원심분리하여 PBS로 헹군 후 남아있는 배지성분을 제거하였다. 이후, PBS를 이용하여 1ⅹ109 CFU/ml 균수로 정량하였으며, 0.1㎖(1ⅹ109 CFU)씩 존대를 이용하여 8주령의 암컷 무모 쥐 모델(female SKH hairless mice)에 UVB 조사 일주일 전부터 매일 경구 투여하였다. 이때 음성 및 양성 대조군에는 멸균된 PBS를 투여하였으며, UVB 처리는 주 3회 무모 쥐의 등 부분에 조사하고, 자외선 조사량은 1주차에 2MED(50mJ/cm2)로 조사를 시작하여 매주 1MED씩 높여서 조사하였다. 실험은 총 12주간 진행하였다.The Lactobacillus plantarum WiKim0060 strain isolated in Example 1 was incubated for 24 hours at 30 ° C. in MRS medium, and the cultured cells were centrifuged at 8,000 rpm for 5 minutes to remove residual media after rinsing with PBS. It was. Subsequently, 1 × 10 9 CFU / ml bacteria were quantified using PBS, and 0.1 ml (1 × 10 9 CFU) were used orally to daily oral administration of 8 weeks old female SKH hairless mice from a week before UVB irradiation. . At this time, sterilized PBS was administered to the negative and positive controls, and UVB treatment was applied to the back part of the hairless rats three times a week, and the amount of ultraviolet radiation was irradiated with 2MED (50mJ / cm 2 ) at week 1 and increased by 1MED every week. Investigate. The experiment was conducted for a total of 12 weeks.
1) UVB 조사 무모 쥐 모델에서의 육안 관찰1) Visual observation of UVB-irradiated hairless rat model
UVB 조사 무모 쥐 모델의 락토바실러스 플란타럼 WiKim0060 처리에 따른 피부를 육안으로 관찰하였다(도 1).Skin following Lactobacillus plantarum WiKim0060 treatment in a UVB irradiated hairless rat model was visually observed (FIG. 1).
그 결과, 락토바실러스 플란타럼 WiKim0060 처리군(WiKim0060)에서 멜라닌 및 홍반 정도가 유의한 수준으로 감소한 것을 확인하였다.As a result, it was confirmed that the melanin and erythema decreased significantly in the Lactobacillus plantarum WiKim0060 treated group (WiKim0060).
2) 멜라닌 정도 측정2) melanin level measurement
락토바실러스 플란타럼 WiKim0060 균주를 실험 동물에 경구투여하여 12주 후, Mexameter(MX18)를 이용하여 멜라닌 정도를 측정하였으며, 실험동물 등 부위의 8곳을 측정하여 그 평균 값을 결과 값으로 산출하였다. After 12 weeks of oral administration of the Lactobacillus plantarum WiKim0060 strain to experimental animals, melanin levels were measured using Mexameter (MX18). .
그 결과, 도 2에서 보는 것과 같이 대조군(C)에 비해 WiKim0060 처리구에서 멜라닌 정도가 유의하게 감소한 것을 확인할 수 있으며, 정상군(N)과 비슷한 수준으로 감소된 것을 확인하였다.As a result, as shown in Figure 2 it can be seen that the melanin was significantly reduced in the WiKim0060 treatment compared to the control group (C), it was confirmed that the level similar to the normal group (N).
3) 홍반 정도 측정3) erythema degree measurement
락토바실러스 플란타럼 WiKim0060 균주를 실험 동물에 경구투여하여 12주 후, Mexameter(MX18)를 이용하여 홍반 정도를 측정하였으며, 실험동물 등 부위의 8곳을 측정하여 그 평균 값을 결과 값으로 산출하였다. After 12 weeks of oral administration of Lactobacillus plantarum WiKim0060 strain to the experimental animals, the erythema was measured using Mexameter (MX18), and the average value of the erythema was measured and the average value was calculated. .
그 결과, 도 3에서 보는 것과 같이 대조군(C)에 비해 WiKim0060 처리구에서 홍반 정도가 유의하게 감소한 것을 확인할 수 있었다.As a result, as shown in Figure 3 it was confirmed that the degree of erythema significantly reduced in the WiKim0060 treatment as compared to the control (C).
4) 경피수분증발량 측정4) Measurement of transdermal moisture evaporation
락토바실러스 플란타럼 WiKim0060 균주를 실험 동물에 경구투여하여 12주 후, Tewameter를 이용하여 경피수분증발량을 측정하였으며, 실험동물 등 부위의 8곳을 측정하여 그 평균 값을 결과 값으로 산출하였다. After 12 weeks of oral administration of Lactobacillus plantarum WiKim0060 strain to experimental animals, transdermal moisture evaporation was measured using a Tewameter, and the average value was calculated as the result value by measuring eight sites of the experimental animals.
그 결과, 도 4에서 보는 것과 같이 대조군(C)에 비해 WiKim0060 처리구에서 경피수분증발량이 감소하는 것을 확인함으로써, 상기 균주 처리에 따른 피부 보습개선 효과를 확인하였다.As a result, as shown in Figure 4 by confirming that the percutaneous moisture evaporation is reduced in the WiKim0060 treatment as compared to the control (C), the effect of improving the skin moisturizing according to the strain treatment.
<110> Korea Food Research Institute <120> Lactobacillus plantarum WiKim0060 having skin whitening and skin moisturizing activities and composition for comprising the same <130> P17R10C1431 <160> 1 <170> KopatentIn 2.0 <210> 1 <211> 759 <212> DNA <213> Lactobacillus plantarum WiKim0060 <400> 1 tgtgaaagcc ttcggctcaa ccgaagaagt gcatcggaaa ctgggaaact tgagtgcaga 60 agaggacagt ggaactccat gtgtagcggt gaaatgcgta gatatatgga agaacaccag 120 tggcgaaggc ggctgtctgg tctgtaactg acgctgaggc tcgaaagtat gggtagcaaa 180 caggattaga taccctggta gtccataccg taaacgatga atgctaagtg ttggagggtt 240 tccgcccttc agtgctgcag ctaacgcatt aagcattccg cctggggagt acggccgcaa 300 ggctgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt 360 cgaagctacg cgaagaacct taccaggtct tgacatacta tgcaaatcta agagattaga 420 cgttcccttc ggggacatgg atacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 480 atgttgggtt aagtcccgca acgagcgcaa cccttattat cagttgccag cattaagttg 540 ggcactctgg tgagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat 600 catgcccctt atgacctggg ctacacacgt gctacaatgg atggtacaac gagttgcgaa 660 ctcgcgagag taagctaatc tcttaaaacc attctcagtt cggattgtag gctgcaactc 720 gcctacatga agtcggaatc gctagtaatc gcggatcag 759 <110> Korea Food Research Institute <120> Lactobacillus plantarum WiKim0060 having skin whitening and skin moisturizing activities and composition for comprising the same <130> P17R10C1431 <160> 1 <170> KopatentIn 2.0 <210> 1 <211> 759 <212> DNA <213> Lactobacillus plantarum WiKim0060 <400> 1 tgtgaaagcc ttcggctcaa ccgaagaagt gcatcggaaa ctgggaaact tgagtgcaga 60 agaggacagt ggaactccat gtgtagcggt gaaatgcgta gatatatgga agaacaccag 120 tggcgaaggc ggctgtctgg tctgtaactg acgctgaggc tcgaaagtat gggtagcaaa 180 caggattaga taccctggta gtccataccg taaacgatga atgctaagtg ttggagggtt 240 tccgcccttc agtgctgcag ctaacgcatt aagcattccg cctggggagt acggccgcaa 300 ggctgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt 360 cgaagctacg cgaagaacct taccaggtct tgacatacta tgcaaatcta agagattaga 420 cgttcccttc ggggacatgg atacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 480 atgttgggtt aagtcccgca acgagcgcaa cccttattat cagttgccag cattaagttg 540 ggcactctgg tgagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat 600 catgcccctt atgacctggg ctacacacgt gctacaatgg atggtacaac gagttgcgaa 660 ctcgcgagag taagctaatc tcttaaaacc attctcagtt cggattgtag gctgcaactc 720 gcctacatga agtcggaatc gctagtaatc gcggatcag 759
Claims (11)
상기 식품은 건강기능식품인 식품 조성물.The method of claim 2,
The food is a dietary supplement food composition.
상기 식품은 음료, 바 또는 발효유인 식품 조성물.The method of claim 2,
The food composition is a beverage, bar or fermented milk.
상기 과색소 침착은 기미, 주근깨 및 흑자(lentigo)로 이루어진 군에서 선택된 하나 이상인 약학 조성물.The method of claim 9,
The hyperpigmentation is pharmaceutical composition of at least one selected from the group consisting of blemishes, freckles and lentigo.
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| KR20220032164A (en) * | 2020-09-07 | 2022-03-15 | 주식회사 휴먼바이오 | Pet snack composition comprising oriental melon and method for manufacturing the same |
| KR20220049069A (en) * | 2020-10-13 | 2022-04-21 | 주식회사 세바바이오텍 | Compositions comprising skin derived lactic acid bacteria having effects of anti-oxidation, anti-inflammation and whitening |
| KR102443180B1 (en) | 2022-05-25 | 2022-09-14 | 주식회사 셀젠느 | High functional cosmetic composition having natural complex extract and ample type liquid cosmetics using the same |
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| CN110151672B (en) * | 2018-02-12 | 2022-04-05 | 景岳生物科技股份有限公司 | Application of lactobacillus plantarum GMNL-6 composition in skin care |
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| KR20220032164A (en) * | 2020-09-07 | 2022-03-15 | 주식회사 휴먼바이오 | Pet snack composition comprising oriental melon and method for manufacturing the same |
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| KR20220049069A (en) * | 2020-10-13 | 2022-04-21 | 주식회사 세바바이오텍 | Compositions comprising skin derived lactic acid bacteria having effects of anti-oxidation, anti-inflammation and whitening |
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