KR102443180B1 - High functional cosmetic composition having natural complex extract and ample type liquid cosmetics using the same - Google Patents

Abstract

The present invention relates to a high-functional cosmetic composition containing a natural complex extract, and ample type liquid cosmeticS using the same. According to the present invention, the high-functional cosmetic composition containing a natural complex extract comprises, based on 100 parts by weight of a Hippophae rhamnoides extract: 40 to 70 parts by weight of Carthamus tinctorius seed oil; 1 to 5 parts by weight of niacinamide; 0.05 to 0.5 part by weight of allantoin; 0.01 to 0.05 part by weight of adenosine; 0.001 to 0.01 part by weight of ceramide; 0.001 to 0.01 part by weight of hydroxydecyl ubiquinone; 0.001 to 0.01 part by weight of at least one peptide mixture selected from a group consisting of acetyl hexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoyl pentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide copper HCL, hexapeptide-9, acetyl tetrapeptide-2, acetyl tetrapeptide-5, and mixtures thereof; 0.01 to 0.05 part by weight of Lactobacillus fermentation lysate; and 1 to 5 parts by weight of purified water. Accordingly, since a natural complex extract is used, toxicity or irritation to the skin does not occur such that skin trouble can be minimized, growth of Staphylococcus epidermidis, which is a beneficial bacterium while being eco-friendly and a common skin bacterium, can be promoted, excellent moisturizing effect can be provided on the skin to increase the amount of moisture in the skin and reduce the amount of dead skin cells and sebum secretion, and can increase skin elasticity to increase effect of wrinkle mitigation and the duration of the effect.

Description

High functional cosmetic composition containing natural complex extract and ampoule type liquid cosmetics using same

The present invention can minimize skin troubles by not toxic or stimulating the skin by using a natural complex extract, and is environmentally friendly, and promotes the growth of Staphylococcus epidermidis, which is a skin flora and beneficial bacteria as well. Rather, it is a natural complex extract that can increase the amount of skin moisture, reduce the amount of dead skin cells and sebum secretion, and improve the elasticity of the skin to improve the effect of wrinkle improvement and increase the duration of these effects due to its excellent moisturizing effect on the skin. It relates to a high-functional cosmetic composition comprising the same and to an ampoule-type liquid cosmetic using the same.

As the economic level of modern people improves and their participation in social life increases, interest in skin beauty and health is increasing to a high level among various age groups. Many cosmetics related to beauty are being developed for the purpose of preventing skin aging and suppressing skin stress to prevent skin aging and maintain clean and healthy skin.

The epidermis, the outermost layer of the skin, plays a role in protecting the human body from the outside. . As an epidermal barrier, the stratum corneum serves as the first line of defense in the body's defense mechanism, which inhibits the evaporation and loss of moisture in the epidermis, prevents the drying of the epidermis, and prevents invasion of the skin by bacteria, fungi, and viruses. In addition, the stratum corneum, that is, the skin barrier, provides an environment in which the skin can perform normal functions by suppressing loss of electrolytes or moisture through the skin.

Therefore, when the skin barrier is damaged, the skin becomes dry and rough and easily exposed to harmful environments, so aggressive chemicals and microorganisms can easily penetrate into the skin and cause problematic skin such as atopic dermatitis, contact dermatitis, psoriasis, dry skin in the elderly, etc. have. As awareness of the importance of the skin barrier increases, skin barrier strengthening has been attracting attention as a new concept in cosmetics and skin care, and treatments for improving the skin barrier function are being studied to treat skin diseases.

In particular, in recent years, research on various cosmetic compositions using natural substances is continued in order not to cause toxicity or irritation to the skin. In addition, development of natural materials, methods of maximizing their efficacy and effects, functional cosmetics with various effects such as hypoallergenic and moisturizing, wrinkle improvement, and increased duration, such as natural plant materials and their fermentation technology Research and development of grafted methods are being actively conducted.

On the other hand, the strain inhabiting the human skin is different for each individual, and may vary according to the nutritional status, health, age, and surrounding environment of each individual. In particular, Staphylococcus epidermidis constitutes more than 90% of the aerobic environment. This bacterium has anti-inflammatory properties and helps to act as a barrier against the growth of pathogenic bacteria such as strains of Staphylococcus aureus. The mechanism of action of S. epidermidis is to prevent the production of cytokines, which are inflammatory substances, by producing antibacterial peptides, Bacteriocins, and also to strengthen the skin barrier in a way that helps the expression of tight junction proteins. It helps the immune system. These actions are mediated through innate immune receptors on keratinocytes and other immune cells. Therefore, like other organs, the skin's innate immune system is due to the interaction between humans and microorganisms, which is an important factor in the homeostasis control of skin immunity.

Accordingly, there is a continuous increase in demand for the development of cosmetics capable of improving the skin condition by promoting the growth of Stephylococcus epidermidis, which is both a skin flora and a beneficial bacterium.

Republic of Korea Patent Registration No. 10-1798505 Republic of Korea Patent Registration No. 10-2070143 Republic of Korea Patent No. 10-2334842

The present invention has been devised to solve the above problems, and an embodiment of the present invention not only promotes the growth of Staphylococcus epidermidis, which is a skin flora and beneficial bacteria, but also has hypoallergenic and skin An object of the present invention is to provide a high-functional cosmetic composition containing a natural complex extract capable of increasing moisturizing, wrinkle improvement and duration for skin and ampoule-type liquid cosmetics using the same.

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

One embodiment of the present invention is based on 100 parts by weight of the vitamin tree extract, 40 to 70 parts by weight of safflower seed oil, 1 to 5 parts by weight of niacinamide, 0.05 to 0.5 parts by weight of allantoin, 0.01 to 0.05 parts by weight of adenosine, 0.001 to ceramide 0.01 parts by weight, hydroxydecylubiquinone 0.001 to 0.01 parts by weight, acetylhexapeptide-8, copper tripeptide-1, palmitoyltripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/ 0.001 to 0.01 parts by weight of one or more peptide mixtures selected from the group consisting of lysine polypeptide copper HCL, hexapeptide-9, acetyltetrapeptide-2, acetyltetrapeptide-5, and mixtures thereof, Lactobacillus fermentation lysate 0.01 to 0.05 parts by weight and 1 to 5 parts by weight of purified water to provide a high-functional cosmetic composition comprising a natural complex extract.

The high-functional cosmetic composition comprising the natural complex extract is based on 100 parts by weight of the vitamin tree extract,

Port marigold flower extract 0.01 to 0.3 parts by weight, dandelion leaf extract 0.005 to 0.05 parts by weight, Centella asiatica extract 0.005 to 0.05 parts by weight, berry complex extract 0.005 to 0.05 parts by weight, golden extract 0.001 to 0.01 parts by weight, Hojang root extract 0.001 to 0.01 parts by weight, 0.001 to 0.01 parts by weight of green tea extract, 0.001 to 0.01 parts by weight of Spanish licorice root extract, 0.001 to 0.01 parts by weight of rosemary leaf extract and 0.001 to 0.01 parts by weight of Matricaria flower extract.

The vitamin tree extract is a mixture of a solvent extract of a vitamin tree, a hot water extract of a vitamin tree, and a fermented extract of a vitamin tree in a weight ratio of 1: 0.5 to 1.5: 0.01 to 0.3;

The solvent extract of the vitamin tree is obtained by pulverizing the seeds of the vitamin tree, mixing with at least one solvent selected from the group consisting of methanol, chloroform, and mixed solvents thereof with 10 to 30 times the weight, and heating, filtering and concentrating them, to be extracted;

The hot water extract of the vitamin tree is to be extracted by mixing the vitamin tree leaves and roots in a 1: 0.1 to 0.3 weight ratio, pulverizing, and mixing them with 200 to 500 times the weight of purified water, heating, filtering and concentrating them. ;

The fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree in a weight ratio of 1: 0.01 to 0.1, crushed, mixed with purified water of 10 to 30 times the weight, sterilized, and then inoculated with a strain and 27 to After culturing and fermenting at 37° C. for 3 to 7 days, they may be extracted by filtration and concentration.

The fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree, crushed, mixed with purified water, sterilized, and before inoculation of the strain;

0.1 to 10 parts by weight of liquid germanium is further mixed based on 100 parts by weight of fruits and branches of the vitamin tree;

The liquid germanium is prepared by a method comprising: pulverizing muscovite containing germanium to have an average particle diameter of 1 to 3 mm, and electrolyzing the pulverized muscovite containing germanium to extract liquid germanium. it could be

On the other hand, another embodiment of the present invention provides an ampoule-type liquid cosmetic comprising a high-functional cosmetic composition comprising the natural complex extract.

According to the high-functional cosmetic composition containing the natural complex extract according to an embodiment of the present invention and the ampoule-type liquid cosmetic using the same, the use of the natural complex extract does not cause toxicity or irritation to the skin, thereby minimizing skin troubles, It has an eco-friendly effect. In addition, there is an effect of promoting the growth of Staphylococcus epidermidis, which is known to be useful for the prevention, improvement, or treatment of skin conditions or inflammation as a skin flora and beneficial bacteria. In addition, it has an excellent moisturizing effect on the skin, increasing the amount of skin moisture, reducing the amount of dead skin cells and sebum secretion, and improving the elasticity of the skin, thereby improving the effect of wrinkle improvement and increasing the duration of these effects. .

Hereinafter, embodiments of the present invention will be described in detail. However, this is provided as an example, and the present invention is not limited thereto, and the present invention is only defined by the scope of the claims to be described later.

As used herein, the term "extract" means that the active ingredient of the extract is not destroyed or is extracted by heating at room temperature or under conditions in which destruction is minimized. At this time, unless otherwise specified, the extraction method for obtaining the extract is not particularly limited, and depending on the type of extract and the extraction method, for example, distillation extraction, cold extraction, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction , ultrasonic extraction, supercritical extraction, and the like can be used. However, the present invention is not limited thereto. In addition, filtration may be performed to remove floating solid particles from the extract obtained through the extraction method. In this case, the filtration may use cotton, nylon, etc. to filter out particles or use ultrafiltration, cryofiltration, centrifugation, etc., but the present invention is not limited thereto. In addition, it may further include a separation process by various chromatography (eg, according to size, charge, hydrophobicity or affinity).

One embodiment of the present invention is based on 100 parts by weight of the vitamin tree extract, 40 to 70 parts by weight of safflower seed oil, 1 to 5 parts by weight of niacinamide, 0.05 to 0.5 parts by weight of allantoin, 0.01 to 0.05 parts by weight of adenosine, 0.001 to ceramide 0.01 parts by weight, hydroxydecylubiquinone 0.001 to 0.01 parts by weight, acetylhexapeptide-8, copper tripeptide-1, palmitoyltripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/ 0.001 to 0.01 parts by weight of one or more peptide mixtures selected from the group consisting of lysine polypeptide copper HCL, hexapeptide-9, acetyltetrapeptide-2, acetyltetrapeptide-5, and mixtures thereof, Lactobacillus fermentation lysate 0.01 to 0.05 parts by weight and 1 to 5 parts by weight of purified water to provide a high-functional cosmetic composition comprising a natural complex extract.

According to such a high-functional cosmetic composition containing a natural complex extract according to an embodiment of the present invention and an ampoule-type liquid cosmetic using the same, by using a natural complex extract, it does not cause toxicity or irritation to the skin, so that skin troubles can be minimized. , has an eco-friendly effect. In addition, there is an effect of promoting the growth of Staphylococcus epidermidis, which is known to be useful for the prevention, improvement, or treatment of skin conditions or inflammation as a skin flora and beneficial bacteria. In addition, it has an excellent moisturizing effect on the skin, increasing the amount of skin moisture, reducing the amount of dead skin cells and sebum secretion, and improving the elasticity of the skin, thereby improving the effect of wrinkle improvement and increasing the duration of these effects. .

More specifically, the vitamin tree extract does not cause toxicity or irritation to the skin, so it is possible to minimize skin troubles, and not only promotes the growth of Staphylococcus epidermidis, which is a common skin flora and beneficial bacteria, It has an excellent moisturizing effect on the skin, increasing the amount of skin moisture, reducing the amount of dead skin cells and sebum secretion, and improving the elasticity of the skin, thereby improving the effect of wrinkle improvement and increasing the duration of these effects.

The vitamin tree extract is prepared according to a conventional method known in the art, that is, using a conventional solvent, conventionally used distillation extraction, chilling, reflux cooling extraction, hot water extraction, room temperature extraction, warming extraction, ultrasonic extraction , can be prepared by methods such as supercritical extraction.

However, the vitamin tree extract is a solvent extract of a vitamin tree, a hot water extract of a vitamin tree, and a fermented extract of a vitamin tree 1: 0.5 to 1.5: By using a mixture of 0.01 to 0.3 weight ratio, the above effect is further improved There is an effect that can be done.

More specifically, the solvent extract of the vitamin tree is powdered with the seeds of the vitamin tree, mixed with at least one solvent selected from the group consisting of methanol, chloroform, and mixed solvents thereof 10 to 30 times by weight, heated and filtered And by concentrating, what is extracted can be used preferably.

At this time, the vitamin tree seeds are washed cleanly, dried at a temperature of 45 to 65 ° C. for 2 to 4 days, and then pulverized to a size of 20 to 50 mesh can be preferably used.

In addition, the solution in which the vitamin tree seed powder is mixed with a solvent is extracted twice or more repeatedly while heating and reflux cooling at a temperature of 60 to 80 ° C. can

In addition, the solvent extract of the vitamin tree is prepared by separately preparing the methanol extract of the vitamin tree seed and the chloroform extract of the vitamin tree seed, respectively, by using a mixture of them in a weight ratio of 1: 0.5 to 1.5, thereby further enhancing the above effect. In addition to being able to improve, in particular, there is an effect that can further improve the growth promoting effect of the beneficial bacteria, Staphylococcus epidermidis (Staphylococcus epidermidis).

In addition, the hot water extract of the vitamin tree is obtained by mixing the vitamin tree leaves and roots in a 1: 0.1 to 0.3 weight ratio, pulverizing, and mixing them with 200 to 500 times the weight of purified water, heating, filtering and concentrating them. can be preferably used.

In this case, the leaves and roots of the vitamin tree are washed cleanly, dried at a temperature of 50 to 80 ° C. for 2 to 4 days, and then crushed to a size of 20 to 50 mesh can be preferably used.

In addition, the solution obtained by mixing the pulverized vitamin tree leaves and roots with purified water is extracted twice or more repeatedly while heating and reflux cooling at a temperature of 85 to 100 ° C., and then filtering and concentrating the extracted mixed solution. It can be used preferably.

In addition, the fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree in a 1: 0.01 to 0.1 weight ratio, crushed, mixed with 10 to 30 times the weight of purified water, sterilized, and then inoculated with a strain After culturing and fermenting at 27 to 37° C. for 3 to 7 days, those extracted by filtration and concentration may be preferably used.

In this case, the vitamin tree fruit and eggplant may be washed cleanly, dried at a temperature of 45 to 65° C. for 1 to 2 days, and then crushed to a size of 20 to 50 mesh may be preferably used.

In addition, the solution of the vitamin fruit and eggplant pulverized product mixed with purified water is heated at a temperature of 85 to 95 ℃ for 5 to 15 minutes, and then sterilized by cooling to a temperature of 40 to 45 ℃, as a fermentation strain, saccharo It is preferable to inoculate 0.1 to 1.0 parts by weight of one or more strains selected from the group consisting of Myces Carlsbergensis, Aspergillus oryzae and mixed strains thereof, based on 100 parts by weight of fruits and branches of the vitamin tree. do.

In addition, the fermented extract of the vitamin tree is the Saccharomyces Carlsbergensis fermented extract of the fruit and eggplant of the vitamin tree, and the fermented extract of Aspergillus oryzae of the fruit and the eggplant of the vitamin tree. By using a mixture in a ratio of 1: 1 to 2, respectively, the above effects can be further improved, and in particular, the effect of enhancing the growth of the beneficial bacteria, Staphylococcus epidermidis, and improving skin elasticity. can be further improved.

In addition, the fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree, crushed, mixed with purified water, sterilized, before inoculation of the strain; By further mixing 0.1 to 10 parts by weight of liquid germanium based on 100 parts by weight of the fruit and eggplant of the vitamin tree, it is possible to further improve the above-mentioned effects, and, in particular, the moisturizing effect on the skin, the amount of keratin on the skin There is an effect that can further improve the effect of reducing excessive sebum secretion and improving skin elasticity.

At this time, the liquid germanium comprises the steps of pulverizing muscovite containing germanium to have an average particle diameter of 1 to 3 mm, and electrolyzing the pulverized muscovite containing germanium to extract liquid germanium; may be manufactured.

At this time, in the electrolysis, an inorganic germanium (Ge) electrode having a purity of 99.9999% or more is provided as the first electrode and the second electrode, and potassium (K), magnesium (Mg), sodium (Na) and a mixture thereof are used as electrolytes. In the electrolysis tank filled with water containing one or more electrolytes selected from the group consisting of, it may be performed by a constant voltage method of 220 to 380v. In addition, the temperature of the electrolyte is 15 to 25 ℃, the pH value is preferably 6.5 to 7.5.

In addition, the step of electrolyzing the pulverized muscovite containing germanium to extract liquid germanium; may be performed by extracting liquid germanium at a concentration of 1000 to 2000 ppm.

Hereinafter, the content of other components constituting the high-functional cosmetic composition comprising the natural complex extract of the present invention is based on 100 parts by weight of the vitamin tree extract.

The safflower seed oil supplies moisture to the skin and maintains moisture to improve the moisturizing effect of the skin, and functions to improve the effect of reducing the amount of dead skin cells and sebum secretion and improving the elasticity of the skin.

The safflower seed oil is washed with safflower seeds, dried at a temperature of 45 to 65 ° C. for 2 to 4 days, and then pulverized to a size of 20 to 50 mesh, put into a mechanical press, and 1200 to 2500 kg / cm ² It can be used more preferably milked at a pressure and a temperature of 45 to 65 ℃.

The safflower seed oil is preferably included in an amount of 40 to 70 parts by weight based on 100 parts by weight of the vitamin tree extract. If the content of the safflower seed oil is too small, there is a problem that the above improvement effect may be insufficient. there is a problem with

The niacinamide is a complex of water-soluble vitamin B, also called nicotinic acid and niacin, and melanocytes, which are components of the skin discoloration, are produced by tyrosinase in the basal layer, the lowest layer of the epidermis, which is from the basal layer to the stratum corneum close to the surface of the skin. Niacinamide can inhibit pigmentation by interfering with this movement, and it can lighten the pigment deposited in the stratum corneum, resulting in a skin whitening effect. In addition, niacinamide may contribute to preventing moisture loss of the skin by increasing the synthesis of intercellular lipids. In addition, it has an antioxidant effect that suppresses free radicals, the cause of skin aging, so that in addition to the whitening effect, which is the main function, it has the effect of enhancing elasticity and improving wrinkles.

The niacinamide is preferably included in an amount of 1 to 5 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of niacinamide is too small, there is a problem that the above-described improvement effect may be insufficient, and when the content of niacinamide is too large, there is a problem that may cause eye irritation.

The allantoin is a natural substance extracted from the buds of camphor, oak, and wheat, which are perennial herbs, and has an excellent skin soothing effect, provides moisture to the skin and maintains moisture to improve the moisturizing effect of the skin, and reduce the amount of dead skin cells and sebum secretion. function to improve the effect.

The allantoin is preferably included in an amount of 0.05 to 0.5 parts by weight based on 100 parts by weight of the vitamin tree extract. If the content of allantoin is too small, the improvement effect may be insufficient, and if the content of allantoin is too high, there is a problem that may irritate the skin.

The adenosine is a compound in which adenine and ribose are combined and is present in small amounts in all living things, including animals, plants, and microorganisms. It can bring effects such as wrinkle improvement, elasticity enhancement, and skin lifting.

The adenosine is preferably included in an amount of 0.01 to 0.05 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of adenosine is too small, there is a problem that the above-described improvement effect may be insufficient, and when the content of adenosine is too large, there is a problem that price competitiveness may be lowered.

The ceramide is a major component of the stratum corneum of the human skin epidermis and constitutes about 50% of the skin. It prevents the entry of contaminants. In addition, it not only gives moisture to dry and rough skin, but also helps to strengthen the skin barrier.

The ceramide is preferably included in an amount of 0.001 to 0.01 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of the ceramide is too small, there is a problem that the above-described improvement effect may be insufficient, and when the content of the ceramide is too large, there is a problem that the ceramide is precipitated and a gelation phenomenon may occur.

The hydroxydecyl ubiquinone is an active oxygen that causes skin aging, and helps to prevent oxidative stress on the skin, and functions to care for the curves of wrinkled skin and to maintain elastic skin.

The hydroxydecyl ubiquinone is preferably included in an amount of 0.001 to 0.01 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of hydroxydecyl ubiquinone is too small, there is a problem that the above-described improvement effect may be insufficient. There is a problem that can be degraded.

The peptide mixture is a non-irritating, non-toxic ingredient raw material, which strengthens the skin barrier and can bring about wrinkle improvement, moisturizing, elasticity enhancement and skin lifting effect. In addition, antioxidant and anti-inflammatory

As such a peptide mixture, acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide copperHCL, hexapeptide- 9, one or more selected from the group consisting of acetyl tetrapeptide-2, acetyl tetrapeptide-5, and mixtures thereof may be preferably used.

More preferably, the peptide mixture is acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide CopperHCL, By using a mixture of hexapeptide-9, acetyltetrapeptide-2 and acetyltetrapeptide-5 in a weight ratio of 4: 4: 2: 4: 4: 2: 2: 1: 1, the above effect is further improved can do.

The peptide mixture is preferably included in an amount of 0.001 to 0.01 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of the peptide mixture is too small, there is a problem that the above-described improvement effect may be insufficient, and when the content of the peptide mixture is too large, there is a problem that price competitiveness may be lowered.

The Lactobacillus ferment lysate functions to improve anti-inflammatory, antioxidant and skin elasticity. In addition, it functions to provide an antibacterial effect against harmful bacteria that cause skin troubles. The Lactobacillus fermentation lysate is obtained by fermenting microorganisms of the genus Lactobacillus, and killing the microorganisms using heat, pH, high pressure of an enzyme (Lysozyme), etc. after their fermentation is completed. It can be preferably used.

At this time, the Lactobacillus genus microorganism is Lactobacillus acidophilus (acidophilus), Lactobacillus fermentum (fermentum), Lactobacillus casei (casei), Lactobacillus reuteri (reuteri), Lactobacillus bifidum (bifidum), Lactobacillus Gasseri, Lactobacillus plantarum, Lactobacillus johnsonii, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus crispatus and their It may be at least one selected from the group consisting of mixed microorganisms, preferably Lactobacillus fermentum, Lactobacillus reuteri, or Lactobacillus paracasei.

The Lactobacillus fermentation lysate is preferably included in an amount of 0.01 to 0.05 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of the Lactobacillus ferment lysate is too small, there is a problem that the above-described improvement effect may be insufficient. There is a problem that can be a stimulus.

The purified water is a natural ingredient that can hydrate the skin, acts as a solvent, and functions to provide a composition having a light and non-greasy feeling.

The purified water is preferably included in an amount of 1 to 5 parts by weight based on 100 parts by weight of the vitamin tree extract. When the content of the purified water is too small, there is a problem that the above-mentioned improvement effect may be insufficient, and when the content of the purified water is too large, there is a problem that the functionality such as the skin barrier strengthening function may be reduced.

In addition, the high-functional cosmetic composition comprising the natural complex extract is a natural preservative and skin conditioning agent, based on 100 parts by weight of the vitamin tree extract, 0.01 to 0.3 parts by weight of port marigold flower extract, 0.005 to 0.05 parts by weight of dandelion leaf extract parts by weight, centella asiatica extract 0.005 to 0.05 parts by weight, berry complex extract 0.005 to 0.05 parts by weight, golden extract 0.001 to 0.01 parts by weight, rhododendron root extract 0.001 to 0.01 parts by weight, green tea extract 0.001 to 0.01 parts by weight, Spanish licorice root extract 0.001 to 0.01 parts by weight, 0.001 to 0.01 parts by weight of rosemary leaf extract, and 0.001 to 0.01 parts by weight of Matricaria flower extract may be further included. Accordingly, there is an effect of further maximizing the growth promoting effect, skin irritation alleviation, elasticity improvement effect and whitening effect of the beneficial bacteria, Staphylococcus epidermidis, by being mixed with the vitamin tree extract.

In this case, the berry complex extract may more preferably include a low sweet blueberry extract, a cranberry extract, a bayberry fruit extract, and a raspberry extract in a weight ratio of 1: 1: 1: 1.

More specifically, the low-sweet blueberry extract, cranberry extract, bayberry fruit extract and raspberry extract are each separately cleanly washed and dried at a temperature of 0 to 5 ° C. Aging for 2 to 4 days in the; adding 0.1 to 0.5 parts by weight of fluranase to 100 parts by weight of berry juice obtained by crushing and juicing the aged berries and enzymatic treatment at 25 to 55° C. for 3 to 7 hours; And it may be prepared by a manufacturing method comprising a; and the step of fermenting the enzyme-treated berry juice at 25 to 35 ° C. for 1 to 2 days, then filtering and concentrating the ferulanase. Accordingly, there is an effect that can be mixed with the vitamin tree extract to further maximize the effect of promoting growth, alleviating skin irritation and improving elasticity of the beneficial bacteria, Staphylococcus epidermidis.

In addition, the high-functional cosmetic composition comprising the natural complex extract may further include 5 to 20 parts by weight of a moisturizing agent with respect to 100 parts by weight of the vitamin tree extract in order to enhance the effect of moisturizing and moisture with a stable viscosity.

These humectants are conventional humectants generally used in the art, and the type is not particularly limited, but non-limiting examples include, but are not limited to, glycerin, ethylhexyl glycerin, dipropylene glycol, methyl propanediol, propanediol, butylene glycol. At least one selected from the group consisting of lycol, propylene glycol, caprylyl glycol, 1,2-hexanediol, and mixtures thereof may be used.

In addition, the high-functional cosmetic composition containing the natural complex extract may further include a cosmetically acceptable additive required for formulation of a formulation or formulated in a skin cosmetic within a range that does not reduce the effect of the present invention. can Non-limiting examples of such additives include known compounds such as oil, water, surfactant, humectant, lower alcohol, emollient, antioxidant, antifoaming agent, preservative, preservative, bactericide, vitamin, thickener, chelating agent, colorant, fragrance, etc. may include, but is not limited thereto.

On the other hand, another embodiment of the present invention provides an ampoule-type liquid cosmetic comprising a high-functional cosmetic composition comprising the natural complex extract.

According to the high-functional cosmetic composition containing the natural complex extract according to an embodiment of the present invention and the ampoule-type liquid cosmetic using the same, the use of the natural complex extract does not cause toxicity or irritation to the skin, thereby minimizing skin troubles, It has an eco-friendly effect. In addition, there is an effect of promoting the growth of Staphylococcus epidermidis, which is known to be useful for the prevention, improvement, or treatment of skin conditions or inflammation as a skin flora and beneficial bacteria. In addition, it has an excellent moisturizing effect on the skin, increasing the amount of skin moisture, reducing the amount of dead skin cells and sebum secretion, and improving the elasticity of the skin, thereby improving the effect of wrinkle improvement and increasing the duration of these effects. .

As mentioned above, although preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various modifications can be made by those skilled in the art within the scope of the technical spirit of the present invention. This is possible.

<Production Example 1>

Methanol Extract of Vitamin Tree Seed

After the vitamin tree seeds were thoroughly washed, dried at a temperature of 55 ° C. for 3 days, and then pulverized to a size of 30 mesh. The vitamin tree seed powder was mixed with 15 times the weight of methanol solvent, and the mixed solution was extracted three times while heating and reflux cooling at a temperature of 75 ° C. of methanol extract was prepared.

<Production Example 2>

Chloroform Extract of Vitamin Tree Seed

A chloroform extract of vitamin tree seeds was prepared in the same manner as in Preparation Example 1, except that a chloroform solvent was used instead of the methanol solvent.

<Production Example 3>

Solvent extract mixture of vitamin tree seeds

By mixing the methanol extract of the vitamin tree seed prepared in Preparation Example 1 and the chloroform extract of the vitamin tree seed prepared in Preparation Example 2 in a 1: 1 weight ratio, a solvent extract mixture of the vitamin tree seed was prepared.

<Production Example 4>

Hot water extract of vitamin tree leaves and roots

After thoroughly washing the leaves and roots of the vitamin tree, dried at a temperature of 75° C. for 2 days, the leaves and roots of the vitamin tree were mixed in a weight ratio of 1: 0.2, and pulverized to a size of 30 mesh. The vitamin tree leaf and root powder was mixed with purified water of 250 times the weight, and the mixed solution was extracted three times while heating and reflux cooling at a temperature of 95 ° C. Then, by filtering and concentrating the extracted mixed solution, the vitamin tree Hot-water extracts of leaves and roots were prepared.

<Preparation Example 5>

Saccharomyces Carlsbergensis Fermented Extract of Vitamin Tree Fruit and Eggplant

After the vitamin tree fruit and eggplant were thoroughly washed and dried at a temperature of 45° C. for 1 day, the vitamin tree fruit and eggplant were mixed in a weight ratio of 1:0.05, and pulverized to a size of 30 mesh. The vitamin tree fruit and eggplant powder was mixed with 25 times the weight of purified water, heated at a temperature of 90 ° C. for 10 minutes, and then sterilized by cooling to a temperature of 45 ° C. As a fermentation strain, Saccharomyces Carlsbergen After inoculating 0.3 parts by weight of the sheath and culturing and fermenting at 35° C. for 7 days, filtration and concentration thereof were performed to prepare a Saccharomyces Carlsbergensis ferment extract of vitamin tree fruit and eggplant.

<Preparation Example 6>

Aspergillus oryzae fermented extract of vitamin tree fruit and eggplant

As the fermented strain, Saccharomyces Carlsbergensis was replaced by Aspergillus oryzae, except that Aspergillus oryzae was fermented into Aspergillus oryzae of vitamin tree fruit and eggplant in the same manner as in Preparation Example 5. Extracts were prepared.

<Production Example 7>

Aspergillus oryzae fermented extract of vitamin tree fruit and eggplant

After the vitamin tree fruit and eggplant were thoroughly washed and dried at a temperature of 45° C. for 1 day, the vitamin tree fruit and eggplant were mixed in a weight ratio of 1:0.05, and pulverized to a size of 30 mesh. The vitamin tree fruit and eggplant powder was mixed with 25 times the weight of purified water, heated at a temperature of 90 ° C. for 10 minutes, and then sterilized by cooling to a temperature of 45 ° C., based on 100 parts by weight of the fruit and eggplant of the vitamin tree. 7 parts by weight of liquid germanium was further mixed. Then, as a fermented strain, 0.3 parts by weight of Aspergillus oryzae was inoculated and cultured and fermented at 35° C. for 7 days, followed by filtration and concentration, thereby obtaining a fermentation extract of Aspergillus oryzae of vitamin tree fruit and eggplant. prepared.

At this time, the liquid germanium was prepared by the following manufacturing method.

After pulverizing muscovite stone containing a lot of germanium to have an average particle diameter of 2 mm, it was put into an electrolysis device and then electrolyzed. At this time, the electrolysis device used an inorganic germanium (Ge) electrode having a purity of 99.9999% or more as the first electrode and the second electrode, and the electrolysis was performed as an electrolyte at 25° C. and pH 7.0 potassium (K) ), in an electrolysis bath filled with water containing magnesium (Mg) and sodium (Na), it was carried out by a constant voltage method of 220v. Thus, 2000 ppm of liquid germanium in a high purity ionic state of 99.9999% by weight was extracted.

<Preparation Example 8>

Fermented extract mixture of vitamin tree fruit and eggplant

The Saccharomyces Carlsbergensis ferment extract of the vitamin tree fruit and eggplant prepared in Preparation Example 5 and the Aspergillus oryzae ferment extract of the vitamin tree fruit and eggplant prepared in Preparation Example 6 were 1: 1.5 weight By mixing in proportions, a mixture of fermented extracts of vitamin tree fruit and eggplant was prepared.

<Production Example 9>

Safflower Seed Oil

After washing the safflower seeds cleanly, drying them at a temperature of 55 ° C. for 3 days, pulverizing them to a size of 40 mesh, putting them in a mechanical press, and milking them at a pressure of 1800 kg/cm ² and a temperature of 60 ° C., safflowers Seed oil was prepared.

<Production Example 10>

Lactobacillus Ferment Lysate

As lactic acid bacteria, 'Lactobacillus permentum' and 'Lactobacillus reuteri' were prepared, respectively. The prepared strain was smeared on MRS solid medium and cultured at 37° C. for 24 hours, and the pure isolated colonies were cultured in MRA liquid medium and mixed with 20% (v/v) glycerol at a ratio of 1: 1 to cryogenic temperature. Stored in the freezer. Thereafter, the strain was cultured in a culture medium and fermented, sterilized with a sterilizer, and impurities were filtered through a 0.2 μm filter to prepare a Lactobacillus fermentation lysate.

<Production Example 11>

Berry Complex Extract

The berries of low-sweet blueberry, cranberry, bayberry fruit and raspberry were separately washed and dried separately, and then aged at 0 °C for 3 days. 0.3 parts by weight of fluranase was added to 100 parts by weight of berry juice obtained by pulverizing each of the aged berries, respectively, and enzyme treatment was performed at 35° C. for 5 hours. After that, each berry juice treated with the flulanase enzyme was fermented at 35 ° C. for 2 days, and then filtered and concentrated to prepare a lowsweet blueberry extract, cranberry extract, bayberry fruit extract and raspberry extract, respectively. .

Then, each of the prepared low-sweet blueberry extract, cranberry extract, bayberry fruit extract and raspberry extract was mixed in a weight ratio of 1: 1: 1: 1 to prepare a berry complex extract.

<Examples and Comparative Examples>

By stirring and mixing the ingredients and contents as shown in Table 1 below, a high-functional cosmetic composition containing a natural complex extract and a composition for comparison were prepared.

Classification (parts by weight) Example 1 Example
2 Example
3 Example
4 Example
5 comparative example
One comparative example
2 comparative example
3 vitamin tree
extract 100 100 100 100 100 - 45 45 (weight ratio) solvent extract One
[Production Example
One] One
[Production Example
2] One
[Production Example
3] One
[Production Example
One] One
[Production Example
3] - - One
[Production Example
One] hot water extract
[Production Example 4] One One One One One - One - Fermented extract 0.2
[Production Example
5] 0.2
[Production Example
6] 0.2
[Production Example
7] 0.2
[Production Example
6] 0.2
[Production Example
8] - - - Safflower Seed Oil
[Production Example 9] 55 55 55 55 55 - - 55 Niacinamide 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 allantoin 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 adenosine 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 ceramide 0.008 0.008 0.008 0.008 0.008 0.008 0.008 0.008 hydroxydecyl
Ubiquinone 0.004 0.004 0.004 0.004 0.004 0.004 0.004 0.004 peptide mixture 0.002 ⁽¹⁾ 0.002 ⁽²⁾ 0.002 ⁽³⁾ 0.002 ⁽²⁾ 0.002 ⁽³⁾ - 0.002 ⁽⁴⁾ 0.002 ⁽⁵⁾ Lactobacillus
Ferment Lysate
[Production Example 10] 0.03 0.03 0.03 0.03 0.03 - - 0.03 Purified water 5 5 5 5 5 100 55 5 port marigold flower extract - - - 0.15 0.15 - - - Western Dandelion Leaf
extract - - - 0.017 0.017 - - - Centella asiatica extract - - - 0.013 0.013 - - - berries
complex extract
[Production Example 11] - - - 0.02 0.02 - - - golden extract - - - 0.007 0.007 - - - rhizome root
extract - - - 0.007 0.007 - - - green tea extract - - - 0.005 0.005 - - - Spanish Licorice Root
extract - - - 0.005 0.005 - - - rosemary leaves
extract - - - 0.003 0.003 - - - matricaria flower
extract - - - 0.002 0.002 - - - glycerin 10 10 10 10 10 10 10 10 caprylyl glycol 5 5 5 5 5 5 5 5 1,2-Hexanediol 5 5 5 5 5 5 5 5 ⁽¹⁾ acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide copperHCL, hexapeptide-9, A mixture of acetyl tetrapeptide-2 and acetyl tetrapeptide-5 in a weight ratio of 1: 1: 1: 1: 1: 1: 1: 1: 1 was used.

⁽²⁾ Acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide copperHCL, hexapeptide-9, A mixture of acetyl tetrapeptide-2 and acetyl tetrapeptide-5 in a weight ratio of 2: 2: 2: 2: 2: 1: 1: 1: 1 was used.

⁽³⁾ Acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide copperHCL, hexapeptide-9, A mixture of acetyl tetrapeptide-2 and acetyl tetrapeptide-5 in a weight ratio of 4: 4: 2: 4: 4: 2: 2: 1: 1 was used.

⁽⁴⁾ A mixture of acetylhexapeptide-8 and hexapeptide-9 in a weight ratio of 2: 1 is used.

⁽⁵⁾ Acetylhexapeptide-8, copper tripeptide-1, palmitoylpentapeptide-4 and tripeptide-1 were mixed in a weight ratio of 1: 1: 1: 1.

Hereinafter, the experimental results comparing the characteristics of the Examples and Comparative Examples according to the present invention are shown so that the characteristics of the high-functional cosmetic composition containing the natural complex extract prepared according to the above Examples can be more easily understood.

<Test Example 1>

The enhancing effect of Staphylococcus epidermidis

A sterile liquid medium was prepared using a commercial TSB (Tryptic soy broth) medium. Staphylococcus epidermidis (Staphylococcus epidermidis) glycerol stock was inoculated into a sterile liquid TSB medium, and pre-cultured at about 35 °C for 12 hours.

5% by weight of each of the high-functional cosmetic composition containing the natural complex extract prepared in Examples to be evaluated and the comparative composition prepared in Comparative Examples to be evaluated in the sterile liquid TSB medium is added, and the pre-culture solution is inoculated, Incubated at about 35 °C for 18 hours. Glucose was added as a positive control. After incubation, the absorbance immediately after inoculation and the absorbance during the stationary phase were measured, and the ratio (growth rate) of the values was used as a statistical variable. By comparing the growth rate of the experimental group treated with the sample compared to the control group not treated with the sample, it was determined whether the sample had a significant effect on the growth of bacteria. The evaluation was performed five or more times, and after calculating the average value and standard deviation of the growth rates of multiple samples, the significance was verified through the t-test.

Example 1 Example
2 Example
3 Example
4 Example
5 comparative example
One comparative example
2 comparative example
3 Beneficial bacteria enhancement effect (%) 23.2 28.5 29.6 31.4 35.9 1.3 4.9 11.6

As can be seen in Table 2, the high-functional cosmetic composition comprising the natural complex extract prepared in Examples is significantly superior to the composition for comparison prepared in Comparative Examples, Staphylococcus epidermidis (Staphylococcus epidermidis) ) was confirmed to exhibit an enhancement effect.

<Test Example 2>

moisturizing effect

The high-functional cosmetic composition containing the natural complex extract prepared in Examples and the comparative composition prepared in Comparative Examples were applied to 5 healthy women in their 40s. The application was applied to an area of about 10 square centimeters on the front curved surface of the left arm, upper arm, and elbow in a constant temperature and humidity room (24 to 26° C., relative humidity 40 to 50%). After one application and absorption, the skin moisture content was measured with a corneometer 2 hours and 4 hours later, and the results are shown in Table 3.

The moisture meter measures the amount of moisture present in the epidermis of the skin using a sensor to measure the amount of moisture ions, and quantifies it and calculates the moisture content to confirm the moisturizing power. do. First, the sensor of the corneometer was attached to the skin surface to be measured and then lightly pressed to display the displayed value, and the average value of the measured values for each subject was used as the measured value. After one measurement, the sensor was wiped with Kimwipe, and the measurement was repeated three times by changing the measurement area. The values shown in the table below are the average of three measurements.

Skin moisture (AU) Example 1 Example
2 Example
3 Example
4 Example
5 comparative example
One comparative example
2 comparative example
3 0 hours 26.8 26.9 27.1 27.0 27.3 26.4 26.5 26.7 2 hours 37.6 37.9 38.5 38.7 39.2 28.7 31.9 34.8 4 hours 38.3 39.7 40.3 40.7 43.8 29.6 32.7 36.2

As can be seen in Table 3, it can be confirmed that the high-functional cosmetic composition comprising the natural complex extract prepared in Examples exhibits remarkably excellent moisturizing power and moisturizing durability compared to the comparative composition prepared in Comparative Examples. there was.

<Test Example 3>

Skin elasticity enhancement effect

For 30 women in their 30s and 40s, 10 women were divided into 3 groups, and measurements were made. The experimental method is as follows. The high-functional cosmetic composition containing the natural complex extract prepared in Examples and the comparative composition prepared in Comparative Examples were applied to each group twice daily for 1 month, and then 1 month later with a skin elasticity meter (cutometer; SEM474, Courage+) Khazaka electronic GmbH Germany) was used to measure the elasticity of the face and compared with that before application, and the results are shown in Table 4 below. The result value in Table 4 means the skin viscoelasticity of the skin elasticity meter, and the value in Table 4 is the average value of three groups.

Example 1 Example
2 Example
3 Example
4 Example
5 comparative example
One comparative example
2 comparative example
3 skin elasticity
Improvement (%) 72 73 76 75 77 35 42 61

As can be seen in Table 4, it was confirmed that the high-functional cosmetic composition containing the natural complex extract prepared in Examples exhibited a remarkably excellent elasticity enhancing effect compared to the comparative composition prepared in Comparative Examples. .

<Test Example 4>

keratin reduction effect

After having 50 adult men and women in their 20s and 30s without skin disease apply the high-functional cosmetic composition containing the natural complex extract prepared in Examples and the composition for comparison prepared in Comparative Examples to the inside of the lower arm, 24 hours After this elapsed, the amount of keratin reduction was measured using the Charm view (Moritex, Japan). The initial amount of dead skin cells and the amount of dead skin cells after 24 hours were measured using the Charm view under constant temperature and humidity conditions (24℃, 40% humidity) before the start of application, and the amount of dead skin cells decreased, and the results are shown in Table 5 below. indicated.

Example 1 Example
2 Example
3 Example
4 Example
5 comparative example
One comparative example
2 comparative example
3 Reduction of skin dead skin cells (%) 9.5 9.9 10.7 11.1 12.3 1.3 2.7 5.3

As can be seen in Table 5, it can be confirmed that the high-functional cosmetic composition comprising the natural complex extract prepared in Examples exhibits a remarkably excellent skin keratin reduction effect compared to the comparative composition prepared in Comparative Examples. there was.

As described above, those skilled in the art to which the present invention pertains will understand that the present invention may be implemented in other specific forms without changing the technical spirit or essential characteristics thereof. Therefore, it should be understood that all of the embodiments described above are illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.

Claims (5)

Based on 100 parts by weight of the vitamin tree extract, 40 to 70 parts by weight of safflower seed oil, 1 to 5 parts by weight of niacinamide, 0.05 to 0.5 parts by weight of allantoin, 0.01 to 0.05 parts by weight of adenosine, 0.001 to 0.01 parts by weight of ceramide, hydroxydecyl 0.001 to 0.01 parts by weight of ubiquinone, acetylhexapeptide-8, copper tripeptide-1, palmitoyl tripeptide-1, palmitoylpentapeptide-4, tripeptide-1, alanine/histidine/lysine polypeptide CopperHCL, 0.001 to 0.01 parts by weight of one or more peptide mixtures selected from the group consisting of hexapeptide-9, acetyltetrapeptide-2, acetyltetrapeptide-5, and mixtures thereof, 0.01 to 0.05 parts by weight of lactobacillus fermentation lysate, and 1 part by weight of purified water A cosmetic composition comprising a natural complex extract, characterized in that it contains to 5 parts by weight.
According to claim 1,
The cosmetic composition comprising the natural complex extract is based on 100 parts by weight of the vitamin tree extract,
Port marigold flower extract 0.01 to 0.3 parts by weight, dandelion leaf extract 0.005 to 0.05 parts by weight, Centella asiatica extract 0.005 to 0.05 parts by weight, berry complex extract 0.005 to 0.05 parts by weight, golden extract 0.001 to 0.01 parts by weight, Hojang root extract 0.001 to 0.01 parts by weight, 0.001 to 0.01 parts by weight of green tea extract, 0.001 to 0.01 parts by weight of Spanish licorice root extract, 0.001 to 0.01 parts by weight of rosemary leaf extract and 0.001 to 0.01 parts by weight of Matricaria flower extract A cosmetic composition comprising a natural complex extract.
According to claim 1,
The vitamin tree extract is a mixture of a solvent extract of a vitamin tree, a hot water extract of a vitamin tree, and a fermented extract of a vitamin tree in a weight ratio of 1: 0.5 to 1.5: 0.01 to 0.3;
The solvent extract of the vitamin tree is obtained by pulverizing the seeds of the vitamin tree, mixing with at least one solvent selected from the group consisting of methanol, chloroform, and mixed solvents thereof with 10 to 30 times the weight, and heating, filtering and concentrating them, to be extracted;
The hot water extract of the vitamin tree is to be extracted by mixing the vitamin tree leaves and roots in a 1: 0.1 to 0.3 weight ratio, pulverizing, and mixing them with 200 to 500 times the weight of purified water, heating, filtering and concentrating them. ;
The fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree in a weight ratio of 1: 0.01 to 0.1, crushed, mixed with purified water of 10 to 30 times the weight, sterilized, and then inoculated with a strain and 27 to A cosmetic composition comprising a natural complex extract, characterized in that it is extracted by culturing and fermenting at 37° C. for 3 to 7 days, then filtering and concentrating them.
4. The method of claim 3,
The fermented extract of the vitamin tree is mixed with the fruits and branches of the vitamin tree, crushed, mixed with purified water, sterilized, and before inoculation of the strain;
0.1 to 10 parts by weight of liquid germanium is further mixed based on 100 parts by weight of fruits and branches of the vitamin tree;
The liquid germanium is prepared by a method comprising: pulverizing muscovite containing germanium to have an average particle diameter of 1 to 3 mm, and electrolyzing the pulverized muscovite containing germanium to extract liquid germanium. A cosmetic composition comprising a natural complex extract, characterized in that.
An ampoule-type liquid cosmetic comprising a cosmetic composition comprising the natural complex extract according to any one of claims 1 to 4.