CN107822945A - One kind is matched unartificial yeast composition, preparation method and applied in cosmetics - Google Patents
One kind is matched unartificial yeast composition, preparation method and applied in cosmetics Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
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- A61Q19/00—Preparations for care of the skin
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/92—Oral administration
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Abstract
One kind is matched unartificial yeast composition and its applied in cosmetics.The present invention provides one kind through long-time Low- temperature culture,Ferment and pass through the match unartificial yeast composition that later stage specially treated obtains,Its raw material sources is naturally pure,Yeast extraction and application rate is high,Product odour effect of optimization is obvious,Additive utilization rate substantially reduces,Contain abundant active dry yeasr in unartificial yeast,The material such as yeast content and yeast product,Amino acid needed by human body can effectively be supplemented,Vitamin,The nutritions such as mineral matter,The match unartificial yeast composition also contains natural plants conditioner and powder raw material simultaneously,Yeast Cultivation can not only be supplied and maintain yeast fermentation,Skin can be nursed one's health with deep layer using in human body skin,Maintain skin health poised state,Other unartificial yeast,Natural plants conditioner and powder raw material combination prepare match unartificial yeast composition in terms of cosmetology have it is a variety of superior the effect of and synergy.
Description
Technical field
The present invention relates to beauty filed of daily-use chemical industry, more particularly to a kind of match unartificial yeast composition, preparation method and
Applied in cosmetics
Background technology
The enhancing of idea is pursued with the improvement of people ' s living standards and to beautiful and health, it is flourishing in fine chemical technology
Development it is current, people be more likely to select natural sex it is purer, safety coefficient is higher, stability is more longlasting, practicality is stronger
Daily chemical product.But the daily chemical product of in the market is a feast for the eyes, and class is also uneven, this allows consumer to be difficult choice.
Yeast is the microorganism that human use is earliest, application is most wide.Yeast cells is by about 35%~50% egg
White matter, 5%~10% nucleic acid, 35%~40% carbohydrate, 2.8%~3.0% lipid, 3%~5% water
Point, 5.5%~6% ash content and a small amount of vitamin composition, wherein carbohydrate include trehalose, glycogen, mannosan,
Portugal is poly- to be warded off with xylan etc..Yeast extract (yeast extract) is that yeast will wherein protein, nucleic acid, dimension after broken wall
The extractings such as raw element, then the active skull cap components such as the amino acid rich in small molecule through biological enzymolysis, peptide, nucleotides, vitamin
Material.Recently as the development of biotechnology, the various use value of yeast constantly are mined out.Yeast is except can be by
By the use of come make wine, make the fermented foods such as bread, as cell protein resource be used for alleviate protein food shortage, make food
Beyond flavor enhancement and making health food, yeast is just increasingly subject to pay attention in the exploitation of cosmetic industry with application.But at present
The yeast used in cosmetics has that yeast sources natural sex is not pure, yeast extraction and application rate is not high, yeast product smell
Bad addition when being difficult to cover, using is few and needs the common problems such as additive urgently to be resolved hurrily.
The content of the invention
To solve the technical problem of existing yeast product, the present invention provides one kind through long-time Low- temperature culture, fermentation and passed through
Later the match unartificial yeast composition that phase specially treated obtains, natural pure, the yeast extraction and application rate height of its raw material sources, product
Smell effect of optimization is obvious, additive utilization rate substantially reduces, and abundant active dry yeasr, yeast content are contained in unartificial yeast
With the material such as yeast product, the nutritions such as amino acid needed by human body, vitamin, mineral matter, while the match can be effectively supplemented
Unartificial yeast composition also contains natural plants conditioner and powder raw material, can not only supply Yeast Cultivation and maintain yeast
Fermentation, skin can be nursed one's health with deep layer using in human body skin, maintain skin health poised state, in addition unartificial yeast, day
Match unartificial yeast composition prepared by right plant regulation agent and powder raw material combination has a variety of superior in terms of cosmetology
Effect and synergy, by add the natural sex that related matrix auxiliary material can make different dosage forms is purer, safety coefficient more
The high, daily chemical product that stability is more longlasting, practicality is stronger.
What the first technical scheme provided by the invention was realized in：
One kind match unartificial yeast composition, said composition include following weight than component：
Wherein, unartificial yeast is active dry yeasr, yeast content and yeast product through cultivating, fermenting, handle, active ferment
Mother is the barms not digested after activating；Yeast content (Yeastextract)：It is that yeast will wherein egg after broken wall
White matter, nucleic acid, vitamin etc. extract, then the amino acid rich in small molecule, peptide, nucleotides, vitamin, mineral through biological enzymolysis
Matter natural active matter, wherein amino acid account for yeast content content more than 30%, and total protein accounts for yeast content more than 50%,
Nucleotides accounts for yeast content more than 10%；Yeast product is active dry yeasr decomposition nutrition matter, including ethanol, titanium dioxide
Carbon, lactic acid；The weight ratio of active dry yeasr, yeast content and yeast product is 1-2 in unartificial yeast:20-30:5-8, combination
Unartificial yeast addition is 0.5-5.0% in thing.
Further, unartificial yeast is that natural plants cultivate fermented bacterium, and the strain is Lu Shi Zygosaccharomyceses
CGMCC2.1915 (parameter represents the numbering of corresponding strain, the adopted name of strain), youth must can Candidas
Any of CGMCC2.1763, saccharomyces cerevisiae CGMCC2.1 and phaffiafhodozyma ATCC66270, every kind of strain addition are equal
For 0.5-5.0%.
Further, plant regulation agent is made up of white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose, and respectively into
It is 3-5 to divide mass ratio:1-3:1-2:1-2:2-3:1-3.And white fungus, gardenia, dragon tree, pseudo-ginseng in plant regulation agent,
Motherwort, sucrose optimum weight ratio 3.5:1.5:1:1:2:1.
Further, natural powder raw material is made up of glucose, hyaluronic acid, polyglutamic acid, collagen, vitamin C,
And mass ratio of each component is 10-15:1-2:1-3:1-2:3-5.And glucose, hyaluronic acid in natural powder raw material,
Polyglutamic acid, collagen, vitamin C press optimum quality ratio 12:1:1.5:1:3.5.
What second of technical scheme provided by the invention was realized in：Match unartificial yeast preparation method of composition：
A, the Lu Shi Zygosaccharomyceses after the preferred activation of unartificial yeast strain, youth must can Candida, saccharomyces cerevisiae and red hairs
Any of husband's yeast, is saved backup；
B, white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose 3-5 in mass ratio:1-3:1-2:1-2:2-3:1-
3 composition plant regulation agent, co-grinding add 10 times of amount water, boiling 2 hours, filtration treatment, filter residue adds 8 times into 1000 grams of coarse powder
Measure water, boiling 1 hour, filtration treatment；Filter residue adds the water of 8 times of amounts again, and boiling 1 hour, filtration treatment, filtrate merges, in 50 DEG C
Under be concentrated under reduced pressure into the clear cream that relative density is 1.0g/ml, in use, plus ethanol make alcohol content up to 60%, stand 24 hours, mistake
Filter, decompression filtrate recycling ethanol is simultaneously concentrated into no alcohol taste, standby after sterilizing；
C, glucose, hyaluronic acid, polyglutamic acid, collagen, vitamin C 10-15 in mass ratio:1-2:1-3:1-
2:3-5 forms natural powder raw material and is well mixed, standby after sterilizing；
D, above-mentioned standby barms is taken under the conditions of constant temperature (25~30 DEG C), constant humidity (50~57%), pH (4.0-7.0)
Unartificial yeast stoste is obtained with the aerobic culture of the sucrose solution shaking table culture or fermentation tank of natural plants conditioner within 1-2 days,
Liquid unartificial yeast is stirred for 25~30 DEG C with powder raw material low temperature, static to place 1-4 days, surveys pH5.0-5.5, terminates hair
Ferment, obtain yeast fermenting mixture；
E, above-mentioned yeast fermenting mixture is taken, adds 0.5g1:1-1:The pectase and extraction from yeast enzyme of 3 mixing, in 50-
60 DEG C of enzymolysis 2-8h, 100 DEG C of stabilization processes 10min, 2000-3000RPM centrifugations, take supernatant with 0.22 μm of miillpore filter mistake
PH5.0-5.5 is adjusted after filter, unartificial yeast composition is as matched, saves backup；
F, above-mentioned match unartificial yeast composition is slowly added into the formulation configured in advance with matrix auxiliary material, gently stirred
Mixing is completely dispersed it；
G, pH4.0-9.0 is adjusted, it is standby that corresponding dosage form product is made.
The third technical scheme provided by the invention is：
Match application of the unartificial yeast composition in cosmetics are prepared, said composition include following weight than component：
Match unartificial yeast composition 1.0%-100.0%
Matrix auxiliary material 0%-99.0%
Wherein, it is claim 1-6 any described to match unartificial yeast composition, and matrix auxiliary material is used for the figuration of product.
Present invention match unartificial yeast composition：Said composition is by unartificial yeast, plant regulation agent, powder raw material by not homogeneity
Measure percentage to be when made under certain process conditions, and add the product that related matrix auxiliary material makes different dosage forms.Main group
Divide respective specific composition, effect and mutual synergetical principle as follows：
(1) unartificial yeast：
Unartificial yeast be natural plants cultivate fermented bacterium, the strain be Lu Shi Zygosaccharomyceses, youth must can Candida,
Any of saccharomyces cerevisiae and phaffiafhodozyma.Unartificial yeast obtains active dry yeasr, in yeast after cultivating, ferment, handle
Tolerant and yeast product, active dry yeasr：The barms not digested after a small amount of activation；Yeast content
(Yeastextract)：It is that yeast wherein protein, nucleic acid, vitamin etc. will extract after broken wall, then the richness through biological enzymolysis
The material of the active skull cap components such as amino acid, peptide, nucleotides, vitamin, trace element containing small molecule, wherein amino acid account for ferment
Female content content more than 30%, total protein account for yeast content more than 50%, and nucleotides, which accounts for yeast content more than 10%, to be had
Have moisturizing, tax living and other effects, it is widely used in cosmetic field, amino acid is natural moisturizing factor (NMF) in keratoderma
Main component, be easily absorbed by the skin, make aging epidermis recover elasticity, delay skin aging；Nucleic acid and nucleotides are human bodies
Central genetic material, have enhance metabolism, improves protein aggregate velocity etc. effect, strengthen immunity function, enhancement of SOD
Activity, improve skin antiradical activities；In addition, the nutrient such as vitamin and mineral a small amount of in product can also be skin
Skin provides sufficient nutrition；Yeast product：A small amount of product of active dry yeasr decomposition nutrition matter is herein referred to, mainly including second
Alcohol, carbon dioxide, lactic acid etc..
(2) plant regulation agent：
Mixed according to a certain percentage in sucrose solution by five kinds of white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort plants,
Clear cream is condensed into, then extracts gained, other active ingredients in the plant regulation agent combined formulation can not only supply yeast training
Yeast fermentation is supported and maintained, skin can be nursed one's health with deep layer, maintain skin health poised state.
White fungus：Polysaccharide compound is the main active of white fungus, and most representational is white fungus acidity different polysaccharide
(Acidicheteroglucan), its structure is using α-mannosan as main chain, with β-(1,2) L- xyloses, β-(1,2) glucose
Aldehydic acid and a small amount of rock algae are side chain.Other several polysaccharide bodies are neutral heteropolysaccharide.s, by xylose, mannose, galactolipin and grape
Aldehydic acid forms.Suppression of the dried tremella extract to collagenase activity, and the facilitation of some biochemical compositions generation is shown
It is shown with the crease-resistant effect of skin-active；Extract has good moisture-keeping function, can be used as NMF；Also there is certain suppression to the allergy of skin
Make and use, can be used as anti-sensitizer of releiving；White fungus is rich in natural characteristic colloid, and plus its nourishing yin effect, long-term use can moisten
Skin, and the effect of dispel face's chloasma, freckle.Antioxidant, NMF, skin conditioning agent can be used as to use.
Gardenia：Also known as cape jasmine, Yellow Fructus Gardeniae, Gentianales Rubiaceae.Gardenia contains Triterpenoid, sour (gradenlicacid) A, B and
Sub- acid.Containing volatile oil, including benzyl acetate, bergamio, another glucoside containing pigment, C6H14O6 etc..In addition, also contain carbon hydrate
Thing, protein, crude fibre and multivitamin.Its benzyl acetate is mainly typically extracted, is the floral perfumes such as fabulous jasmine type
And fragrance for detergents.Extract has anti-oxidant, the effect of skin-active, available for anatrophic；There is anti-inflammatory simultaneously, can also use
Make NMF.With skin state is improved, the effect of skin health, while pleasant aroma are nursed one's health, is easy to receive.
Dragon tree：Dragon tree extract contains thaspine (Taspine) and former cyanine (PAC).Thaspine has very
Strong inoxidizability, skin keratin barrier can also be strengthened, and repair the facial obscure, colour of skin caused by aging and external injury
Uneven skin, significantly improves the colour of skin and skin pattern；OPC is that internationally recognized removing human free radical most has at present
The natural of effect.It potent can resist inflammation and repair damage, while establish skin moisture barrier, improve red blood silk
And sensitivity.There is anti-oxidant, anti-aging to skin and improve the colour of skin.
Pseudo-ginseng：Chinese medicine name.Sweet, slight bitter, temperature.There is promotion microcirculation, promote the synthesis of neoblast, anti-inflammatory etc. to act on.To floating
It is swollen to have suppression, also there is suppression to nuclear factor receptor activation, this is an index for reflecting anti-inflammatory power, and Notogineng Extract can be used as
Antiinflammatory；Notogineng Extract can eliminate oxygen radical, strengthen the activity of endogenous Sudismase, and it is free to oxygen to strengthen body
The Scavenging activity of base, mitigate chain reaction caused by oxygen radical, reduce the peroxidating of lipid in blood；Notogineng Extract has antibacterial
Property, arasaponin has stronger inhibitory action to some fungies, there is certain suppression to staphylococcus aureus, Escherichia coli
Effect.There is anti-inflammatory, anti-oxidant and antibacterial to skin.
Motherwort：Extract has good facilitation to NMF generation, has moisture-keeping function to skin；To B-
16 cells have good inhibiting rate, therefore available for preventing and treating freckle, cutaneous pigmentation；With inoxidizability, with reference to paddy Guang
The activation of sweet fabk polypeptide, it is believed that its is anti-ageing.To human body skin moisturizing, anti-oxidant and to highlight effect of dispelling spots obvious.
Sucrose：It is the small molecule nutriment and wetting agent that content is high in beet, sugarcane and fruit, there is good guarantor
Wet effect, certain density sucrose solution have osmosis, and active ingredient can be promoted to rapidly enter skin and play effect and make
With, while the sucrose solution of high concentration can also suppress harmful microbe growth.Mainly nutrition, moisturizing, rush ooze, antibacterial work
With.
(3) natural powder raw material：
Made according to a certain percentage with method collocation by glucose, hyaluronic acid, polyglutamic acid, collagen and vitamin C
With, can not only supply Yeast Cultivation and maintain yeast fermentation, also with certain collaboration moisture retention, can with deep layer reparation and
Skin is nourished, has certain efficacy effect to skin and mucosa and skin.
Glucose：Referred to as glucose, chemical name are 2,3,4,5,6- penta hydroxy group hexanals.Be distributed in nature most extensively and most
For a kind of important monose, it is a kind of polyhydroxy aldehyde.It is the energy source of living cells and metabolic intermediate product, i.e. biology
Main Energy supply material.Add in cosmetics can produce moisturizing, stably, improve the functions such as the colour of skin, anti-aging and antibacterial.
Hyaluronic acid：Also known as Hyaluronic Acid, it is a kind of high molecular polymer, by unit D-Glucose aldehydic acid and N- acetyl Portugal
The advanced acid mucopolysaccharide of osamine composition, it is the tender important foundation material of skin water, itself is also a kind of composition of human body, it
With special water retention, deal is more up to 100 times of itself weight, is that moisture retention is best in the nature having now been found that
Material, it can also improve skin-nourishing metabolism, make skin it is tender, it is smooth, go to wrinkle, increase it is elastic, prevent aging, in moisturizing
While be good skin penetration enhancer again, be used cooperatively with other nutritional ingredients, can play and promote nutrient absorption
More preferable effect.
Polyglutamic acid：It is reported that polyglutamic acid (γ-PGA) is a kind of water-soluble, biodegradable, use without toxicity
Boiomacromolecule made from microbe fermentation method, it is a kind of sticking material, is found first in " natto ".γ-PGA
It is a kind of special anion nature polymer, is using the glutamic acid of left and right optical activity as cell cube, in alpha-amido and γ-carboxylic
Formed homotype polyamide polypeptide is bonded between base through acid amides, γ-PGA molecular weight is from 5 ten thousand to 2 megadaltons.
Special molecular structure, extremely strong moisture-retaining capacity is made it have, can effectively increase the moisture-retaining capacity of skin, promote skin to be good for
Health, its superpower moisture-retaining capacity are better than common moisturizing ingredient, for the biotechnology NMF of a new generation.
Collagen：Efficient moisture-retention composition, skin-active, inducing tissue regeneration, the main bonding material in human connective tissue,
Molecular weight is big, moisture-retaining capacity compared with polyalcohols more preferably, mainly to support cell, especially suitable for after skin exfoliating, skin
The physiotherapy made in the most thin stage is protected.Collagen has good permeability and moisturizing power, is mainly used in more scar, anti-
Wrinkle, the conditioner of pachylosis patient.Collagen and its hydrolysate compatibility are strong, can coordinate with many functional components to carry
It is high they the effect of, use can effective hair conditioning in hair product.
Vitamin C：Vitamin C is natural antioxidant in cosmetics, for preventing and treating skin sunburn.Human body sheet
Body can not produce Vc, it is necessary to be absorbed from the external world.In cosmetics, Vc passes through mutual in tyrosinase moving part with copper ion
Effect is to reduce the multistep such as DOPA quinone melanin synthesis process to disturb the generation of melanin, the effect with good whitening spot-removing
Fruit.Also there is anti-inflammatory simultaneously.
(4) matrix auxiliary material：The cosmetic product of different dosage forms can be made by adding related matrix auxiliary material, matrix auxiliary material is excellent
Select deionized water, glycerine, butanediol, glycine betaine, glycerin polyether -26, dipotassium glycyrrhizinate, parahydroxyacet-ophenone, 1,2- hexylene glycols,
Trehalose, Cremophor RH40, potassium hydroxide, EDTA- disodiums, pentanediol, PEG-4000, carbomer, ethoxy
Cellulose, lipoic acid, hydrogenated olive oil, triethanolamine, essence, isononyl isononanoate, saualane, dimethyl silicone polymer,
MONTANOV L, SIMULGEL EG, niacinamide, tocopherol, bisabolol, acetyl chitosamine, myristic acid, palmitic acid, tristearin
Acid, potassium hydroxide, LC, laurate, methyl hydroxybenzoate polyethylene glycol -14M, guar hydroxypropyltrimonium chloride, D-pantothenyl aleohol, chlorine
Change sodium, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, Cosmocil CQ, PLURONIC L64,0.05%GREEN 6, isomery hexadecane,
Ring five dimethyl silicone polymer, sorb (sugar) alcohol, honey powder, peppermint, potassium sorbate, sodium benzoate, ascorbic acid, laruyl alcohol sulphur
Acid esters sodium, hydrated silica, xylitol, chitosan, sodium alginate, saccharin sodium, microcrystalline cellulose, stevioside, sodium lauroyl glutamate,
Sodium acid carbonate, gelatin, mannitol, maltose, sweet orange juice, blueberry juice, grape juice, vitamin C, lactose, maltodextrin, amino
Acid, radix glycyrrhizae, magnesium stearate, silica, tea extract, acesulfame potassium, propane diols, Tween-80, F-68, Finasteride,
K220, AES, CAB, 6501, pearly-lustre dibasic acid esters, silicone emulsion, guar gum C-14-S, citric acid, shredding, aloe extract, 18
Alcohol, cationic surfactant, lanolin, phenmethylol, pigment, peregal, A25, EP-200, cera alba, methyl esters, propyl ester, sheep
Hair fat, VA64, sodium methyl cocoyl taurate, LAA, sodium lauroyl sarcosine, coconut oleoyl amine methyl
MEA, decyl glyceryl monooleate, Natrulon H-10 oleate, caprylic/capric glyceride type Natrulon H-10 esters, PEG-120 first
It is base glucose dioleate, cocounut oil acyl isethionic acid ester sodium, lauryl glucoside, MS621, Polyquaternium-10, sodium citrate, sweet
It is oily laurate, dehydroactic acid, caprylic/capric glyceride, shea butter, white oil, cetostearyl alcohol, silicone emulsion, AVC, sweet
Oleostearin acid esters, polysorbate -60, stereth -21, olive oil, cyclopentasiloxane, lecithin, Phenoxyethanol, ethyl
Hexyl glycerine, stearic acid, hexadecanol, private disk -60, K12, glycerine, phenyl salicylate, titanium dioxide, coconut monoethanolamide base propyl group sweet tea
Dish alkali, DOW, sodium chloride, ketoconazole, boric acid, ethyl hydroxy benzoate, SP-115B, parachlorometaxylenol, lactic acid, sodium lactate, borax,
Macrogol 4000, tartaric acid, ethanol, ethoxy urea, 1618 alcohol, M68, A165, isopropyl isostearate, DC1401, fertility
More than one or both of phenol acetate, for the figuration of product and other effects.
In a word, advantage of the present invention is as follows：
1st, the unartificial yeast matched in unartificial yeast composition be barms constant temperature (25~30 DEG C), constant humidity (50~
57%), the culture medium formed under the conditions of pH (4.0-7.0) with the sucrose solution of natural plants conditioner cultivates unartificial yeast original
Liquid, liquid unartificial yeast and powder raw material low temperature are stirred, allow yeast to ferment in the state of without prejudice to yeast activity
Certain time, then handled to obtain by certain process conditions, natural pure, the yeast extraction and application rate of its raw material sources
Height, product odour effect of optimization are obvious, additive utilization rate substantially reduces, i.e., unartificial yeast obtains after cultivating, ferment, handle
The materials such as active dry yeasr, yeast content and yeast product, it can effectively supplement amino acid needed by human body, vitamin, mineral matter
Etc. nutrition, product stability, optimization product odour, increase product work(can be improved through long-time cold fermentation in process
Valence value, the use for reducing additive.
2nd, natural plants conditioner, the powder raw material added in unartificial yeast composition is matched, can not only supply yeast training
Yeast fermentation is supported and maintained, skin can be nursed one's health with deep layer using in human body skin, maintain skin health poised state, separately
Match unartificial yeast composition prepared by outer unartificial yeast, natural plants conditioner and powder raw material combination has in terms of cosmetology
There are the effect of a variety of superior and synergy, specifically have the beneficial effect that：It is caused a large amount of in active yeast metabolic process
Enzyme is converted and decomposed to the plant regulation agent and powder raw material, macromolecular substances is changed into the small molecule thing for being easy to absorb
Matter；Meanwhile plant regulation agent and the nutrient matrix of the growth for being incorporated as active dry yeasr of powder raw material and fermentation offer abundance, turn
Small molecule after changing and decomposing provides the micro-ecological environment for keeping active and comfortable delicate fragrance for a long time for active dry yeasr again；In addition,
The addition of natural powder raw material can also effectively facilitate having in yeast content, yeast product and Plant Transformation analyte
Effect composition rapid osmotic enters skin, the metabolism of human body skin is participated in, to reach more preferable cosmetology effect.
3rd, after natural plants conditioner is concentrated in sucrose solution, extracted, work of the active component therein in sucrose molecule
With lower tight clusters together, Candida albicans, staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa can efficiently be suppressed
Deng harmful bacteria, so as to reduce as the skin and mucosa infection caused by them and the generation of inflammatory reaction；In plant regulation agent simultaneously
Active ingredient skin and mucosa can also be promoted to secrete functional component, and then the inflammatory stimulus for suppressing inflammation and anti-endotoxin are made
With, reach enhancing skin and mucosa immunity effect；Plant regulation agent, which includes plurality of active ingredients, can adjust sebum secretion, suppression
Melanin processed produces, removes free radical, stimulation of releiving, and with reference to related efficacy composition skin can be made to reach more preferably shape
State, releived etc. in replenishing water and preserving moisture, bright color, antioxidant and anti-aging and calmness and reach synergy；Certain density sugarcane
Sugar juice has osmosis, and active ingredient can be promoted to rapidly enter skin and play efficacy effect.
4th, each composition in natural powder raw material can help skin to mend after certain proportion and method complex treatment obtain
Water moisturizing, bright color, anti-inflammatory, antiallergy, releive and promote cell repair；Ingredient stability is good simultaneously, and skin has very well
Compatibility, reduce scytitis reaction, prevent allergic generation, improve the anti-irritant ability of skin；Powder raw material combines
There is related efficacy composition significant collaboration to strengthen efficacy effect, so as to preferably carry out deep layer nourishing to skin, maintain
The optimal health status of skin.
5th, present invention match unartificial yeast composition can make the natural sex of different dosage forms by adding related matrix auxiliary material
Daily chemical product purer, safety coefficient is higher, stability is more longlasting, practicality is stronger, including but not limited to toner, face
Film, anti-acne elite, creams, mildy wash, make up remover, plain face frost, shampoo, hair conditioner, shower cream, bath effervescent tablets, body lotion,
Suncream, Oropharynx spray, toothpaste, tooth piece, oral liquid, meals soft capsule, private portion's foam, private portion's gel.
Brief description of the drawings
Fig. 1 is percutaneous absorbability experimental result picture.
Embodiment
To describe the technology contents of the present invention, construction feature, the objects and the effects in detail, below in conjunction with embodiment
And accompanying drawing is coordinated to be explained in detail.
1. plan content
A kind of 1.1 match unartificial yeast compositions
Said composition include following weight than component：Unartificial yeast 0.5-5.0%, plant regulation agent 50.0-80.0%,
Powder raw material 1.5-5.0%, deionized water 10.0-48.0%；Wherein, unartificial yeast is that natural plants cultivate fermented bacterium, institute
State strain for Lu Shi Zygosaccharomyceses CGMCC2.1915, youth must can Candida CGMCC2.1763, saccharomyces cerevisiae CGMCC2.1 and
Any of phaffiafhodozyma ATCC66270, and unartificial yeast is active dry yeasr, yeast content through cultivating, fermenting, handle
Thing and yeast product, active dry yeasr：The barms not digested after a small amount of activation；Yeast content
(Yeastextract)：It is that yeast wherein protein, nucleic acid, vitamin etc. will extract after broken wall, then the richness through biological enzymolysis
The material of the active skull cap components such as amino acid, peptide, nucleotides, vitamin, mineral matter containing small molecule, wherein amino acid account for yeast
Content content more than 30%, total protein account for yeast content more than 50%, and nucleotides accounts for yeast content more than 10%；Yeast
Fermentate：Herein refer to a small amount of product of active dry yeasr decomposition nutrition matter, including ethanol, carbon dioxide, lactic acid；In unartificial yeast
The weight ratio of active dry yeasr, yeast content and yeast product is 1-2:20-30:5-8, unartificial yeast addition in composition
For 0.5-5.0%.
Above-mentioned plant regulation agent is made up of white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose, and each composition
Mass ratio is 3.5:1.5:1:1:2:1.
Above-mentioned natural powder raw material is made up of glucose, hyaluronic acid, polyglutamic acid, collagen, vitamin C, and
Mass ratio of each component is 12:1:1.5:1:3.5.
A kind of 1.2 match unartificial yeast preparation method of composition
A, the Lu Shi Zygosaccharomyceses CGMCC2.1915 after the preferred activation of unartificial yeast strain, youth must can Candidas
Any of CGMCC2.1763, saccharomyces cerevisiae CGMCC2.1 and phaffiafhodozyma ATCC66270, are saved backup；
B, white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose 3-5 in mass ratio:1-3:1-2:1-2:2-3:1-
3 composition plant regulation agent, wherein each composition optimum weight ratio 3.5:1.5:1:1:2:1, co-grinding adds 10 into 1000 grams of coarse powder
Amount water again, boiling 2 hours, filtration treatment, filter residue add 8 times of amount water, boiling 1 hour, filtration treatment；Filter residue adds the water of 8 times of amounts again,
Boiling 1 hour, filtration treatment, filtrate merge, and the clear cream that relative density is 1.0g/ml is concentrated under reduced pressure at 50 DEG C, in use,
Ethanol is added alcohol content is stood 24 hours, filtering, decompression filtrate recycling ethanol is simultaneously concentrated into no alcohol taste, and sterilize standby up to 60%
With；
C, glucose, hyaluronic acid, polyglutamic acid, collagen, vitamin C 10-15 in mass ratio:1-2:1-3:1-
2:3-5 forms natural powder raw material, by optimum quality ratio 12:1:1.5:1:3.5 weigh glucose, hyaluronic acid, poly- paddy respectively
Propylhomoserin, collagen and vitamin C are well mixed, standby after sterilizing；
D, above-mentioned standby barms is taken under the conditions of constant temperature (25~30 DEG C), constant humidity (50~57%), pH (4.0-7.0)
Unartificial yeast stoste is obtained with the aerobic culture of the sucrose solution shaking table culture or fermentation tank of natural plants conditioner within 1-2 days,
Liquid unartificial yeast is stirred for 25~30 DEG C with powder raw material low temperature, static to place 1-4 days, surveys pH5.0-5.5, terminates hair
Ferment, obtain yeast fermenting mixture；
E, above-mentioned yeast fermenting mixture is taken, adds 0.5g1:1-1:The pectase and extraction from yeast enzyme of 3 mixing, in 50-
60 DEG C of enzymolysis 2-8h, 100 DEG C of stabilization processes 10min, 2000-3000RPM centrifugations, take supernatant with 0.22 μm of miillpore filter mistake
PH5.0-5.5 is adjusted after filter, unartificial yeast composition is as matched, saves backup；
F, above-mentioned match unartificial yeast composition is slowly added into the formulation configured in advance with matrix auxiliary material, gently stirred
Mixing is completely dispersed it；
G, pH4.0-9.0 is adjusted, it is standby that corresponding dosage form product is made.
A kind of 1.3 match unartificial yeast compositions are applied in cosmetics
1.3.1 unartificial yeast composition (part by weight) is matched
Unartificial yeast composition 70.0% (unartificial yeast 2.0%, plant regulation agent 65.0%, powder raw material 3.0%) is matched,
Add deionized water to 100.
1.3.2 unartificial yeast composition conditioning spraying (part by weight) is matched
Match unartificial yeast composition 5.0%, glycine betaine 1.0%, glycerin polyether -26 2.0%, dipotassium glycyrrhizinate 0.1%,
Parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%, Cremophor RH40 0.1%, potassium hydroxide 0.015%, EDTA-
Disodium 0.05%, add deionized water to 100.
1.3.3 unartificial yeast composition facial mask (part by weight) is matched
Match unartificial yeast composition 10.0%, glycerine 5.0%, butanediol 3.0%, pentanediol 1.5%, PEG-4000
1.0%th, glycerin polyether -26 1.0%, D-sorbite 0.2%, carbomer 0.15%, hydroxyethyl cellulose 0.08%, lipoic acid
0.01%th, dipotassium glycyrrhizinate 0.1%, hydrogenated olive oil 0.1%, parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%, PEG-
40 rilanit specials 0.1%, triethanolamine 0.14%, EDTA- disodiums 0.05%, essence 0.01%, add deionized water to 100.
1.3.4 unartificial yeast composition anti-acne elite (part by weight) is matched
Match unartificial yeast composition 25.0%, glycerine 4.0%, butanediol 3.0%, niacinamide 3.0%, sucrose 2.0%, general
Alcohol 1.5%, salicylic acid 1.0%, decoyl hydroximic acid 0.7%, 1,2- hexylene glycols 0.3%, 3-o- ethylascorbyls 0.3%, life
Educate phenol 0.5%, bisabolol 0.1%, hydroxyethyl cellulose 0.2%, cellulose gum 0.4%, dipotassium glycyrrhizinate 0.05%, EDTA
Disodium 0.05%, Solubilisant LRI 5.0%, add deionized water to 100.
1.3.5 unartificial yeast composition emulsion (part by weight) is matched
Match unartificial yeast composition 8.0%, glycerine 5.0%, butanediol 3.0%, isononyl isononanoate 4.0%, saualane
2.0%th, glycerin polyether -26 1.0%, dimethyl silicone polymer 1.0%, MONTANOV L 0.6%, SIMULGEL EG
0.5%th, niacinamide 0.5%, tocopherol 0.1%, bisabolol 0.2%, card ripple 0.18%, triethanolamine 0.14%, acetyl shell
Osamine 0.1%, parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%, EDTA- disodiums 0.05%, essence 0.02%, add from
Sub- water is to 100.
1.3.6 the clear acne Cleansing Foam (part by weight) of unartificial yeast composition is matched
Match unartificial yeast composition 6.0%, glycerine 25.0%, myristic acid 17.0%, palmitic acid 7.5%, stearic acid
7.3%th, potassium hydroxide 7.2%, LC 3.0%, laurate 2.0%, methyl hydroxybenzoate 0.2%, essence 0.5%, polyethylene glycol -14M
0.1%th, EDETATE SODIUM 0.1%, guar hydroxypropyltrimonium chloride 0.07%, add deionized water to 100.
1.3.7 it is white (part by weight) to match unartificial yeast composition element face
Match unartificial yeast composition 3.0%, glycerine 8.0%, glycine betaine 2.0%, ethoxy urea 7.0%, panthenol 0.1%,
Methyl esters 0.2%, EG0.8%, 1618 alcohol 1.0%, M68 2.0%, A165 1.0%, dimethyl silicone polymer 2.0%, allantoin
0.2%th, shea butter 1.5%, isopropyl isostearate 6.0%, DC14011.0%, white oil 3.0%, tocopherol acetate
0.5%th, propyl ester 0.1%, titanium dioxide 2.5%, isononyl isononanoate 3.0%, add deionized water to 100.
1.3.8 unartificial yeast composition make up remover (part by weight) is matched
Match unartificial yeast composition 1.0%, D-pantothenyl aleohol 0.7%, sodium chloride 0.55%, potassium dihydrogen phosphate 0.1%, phosphoric acid hydrogen
Dipotassium 0.1%, Cosmocil CQ 0.7%, PLURONIC L64 0.04%, 0.05%GREEN 6 0.38%, isomery 16
Alkane 10.0%, D5 16%, add deionized water to 100.
1.3.9 unartificial yeast composition shampoo (part by weight) is matched
Match unartificial yeast composition 5.0%, AES (70%) 18%, CAB (30%) 5.0%, 6,501 2.0%, pearly-lustre pair
Ester 1.5%, silicone emulsion (60%) 2.0%, guar gum C-14-S 0.3%, citric acid 0.2%, sodium chloride 0.8%, shredding
(1.5%) 0.08%, essence 0.5%, EDTA- disodiums 0.1%, add deionized water to 100.1.3.10 match unartificial yeast composition
Hair conditioner (part by weight)
Match unartificial yeast composition 10.0%, aloe extract 20.0%, octadecyl alcolol 5.0%, cationic surfactant
8.0%th, lanolin 2.0%, sodium benzoate 0.2%, phenmethylol 0.3%, sodium chloride 1.0%, essence 0.02%, pigment
0.01%, add deionized water to 100.
1.3.11 unartificial yeast composition pomade (part by weight) is matched
Match unartificial yeast composition 1.5%, peregal 25.0%, A25 1.0%, EP-200 2.6%, cera alba
8.0%th, methyl esters 0.2%, propyl ester 0.1%, lanolin 1.0%, propane diols 10.0%, VA64 2.0%, essence 0.02%, add
Ionized water is to 100.
1.3.12 unartificial yeast composition shower cream (part by weight) is matched
Match unartificial yeast composition 6.0%, sodium methyl cocoyl taurate 6.0%, LAA
6.0%th, sodium lauroyl sarcosine 5.0%, coconut oleoyl amine methyl MEA3.0%, decyl glyceryl monooleate 2.0%, polyglycereol-
10 oleates 2.0%, caprylic/capric glyceride type Natrulon H-10 esters 2.0%, PEG-120 methyl dioleates
1.5%th, cocounut oil acyl isethionic acid ester sodium 1.0%, lauryl glucoside 1.0%, MS621 1.0%, Polyquaternium-10
0.4%th, sodium citrate 0.1%, EDETATE SODIUM 0.1%, glycerol monolaurate 0.3%, citric acid 0.3%, panthenol 0.5%, de-
Hydroacetic acid 0.5%, phenmethylol 0.3%, essence 0.25%, add deionized water to 100.
1.3.13 unartificial yeast composition bath effervescent tablets (part by weight) are matched
Match unartificial yeast composition 30.0%, citric acid 20.0%, sodium acid carbonate 20.0%, sodium chloride 8.5.%, sorb
Alcohol 8.0%, peppermint 5.0%, Macrogol 4000 2.0%, amino acid foaming agent 5.0%, sodium benzoate 0.5%, ethanol
0.5%th, essence 0.5%.
1.3.14 unartificial yeast composition body lotion (part by weight) is matched
Match unartificial yeast composition 15.0%, glycerine 5.0%, caprylic/capric glyceride 5.0%, shea butter 3.0%,
Butanediol 3.0%, white oil 2.0%, cetostearyl alcohol 1.0%, silicone emulsion 1.0%, AVC0.6%, isononyl isononanoate
1.0%th, stearine 1.0%, polysorbate -60 2.0%, stereth -21 0.5%, tocopherol 0.5%,
Panthenol 0.5%, olive oil 0.5%, cyclopentasiloxane 0.2%, lecithin 0.2%, Phenoxyethanol 0.3%, Sensiva SC50
0.2%th, EDTA- disodiums 0.1%, deionized water is added to 100.
1.3.15 unartificial yeast composition suncream (part by weight) is matched
Match unartificial yeast composition 2.0%, stearic acid 10.0%, hexadecanol 1.5%, lanolin 1.0%, white oil 5.0%,
Glycerin monostearate 0.5%, Tween-80 2.7%, private disk -60 0.5%, K12 0.3%, glycerine 2.5%, salicylic acid benzene
Ester 1.0%, titanium dioxide 1.0%, add deionized water to 100.
1.3.16 unartificial yeast composition oropharynx maintenance spray (part by weight) is matched
Match unartificial yeast composition 10.0%, glycerine 2.0%, sorb (sugar) alcohol 2.0%, honey powder 0.5%, peppermint
0.1%th, potassium sorbate 0.5%, sodium benzoate 0.2%, add deionized water to 100.
1.3.17 unartificial yeast composition care toothpaste (part by weight) is matched
Match unartificial yeast composition 8.0%, ascorbic acid 1.0%, laruyl alcohol sodium sulfovinate 2.0%, hydrated silica 18%,
Sorb (sugar) alcohol 30%, xylitol 5.0%, polyethylene glycol 400 5.0%, chitosan 1.0%, hydroxyethyl cellulose 0.5%, sea
Mosanom 3.0, peppermint 1.5%, saccharin sodium 0.2%, add deionized water to 100.
1.3.18 match unartificial yeast composition is brushed teeth tooth piece (part by weight)
Match unartificial yeast composition 5.0%, microcrystalline cellulose 25.5%, sorb (sugar) alcohol 30.5%, xylitol 25.0%, sweet tea
Synanthrin 0.25%, sodium lauroyl glutamate 1.0%, peppermint 5.0%, sodium alginate 3.0%, sodium acid carbonate 1.0%, ascorbic acid
1.0%th, radix glycyrrhizae 0.5%, magnesium stearate 1.0%, silica 0.5, essence 0.75%.
1.3.19 unartificial yeast composition oral liquid (part by weight) is matched
Match unartificial yeast composition 2.5%, sweet orange juice 5.0%, blueberry juice 2.0%, grape juice 2.0%, vitamin C
1.0%th, lactose 0.2%, maltodextrin 0.2%, amino acid 0.1%, sodium chloride 0.1%, add deionized water to 100.
1.3.20 unartificial yeast composition meals soft capsule (part by weight) is matched
Match unartificial yeast composition 4.0%, gelatin 42%, glycerine 23%, sorb (sugar) alcohol 5.0%, mannitol
1.5%th, maltose 2.5%, pigment 0.1%, PEG400 28%, add deionized water to 100.
1.3.21 unartificial yeast composition private portion's foam (part by weight) is matched
Match unartificial yeast composition 10.0%, sodium lauroyl sarcosine 6.0%, SP-115B3.0%, lauroyl both sexes base
Sodium acetate 2.0%, glycerine 1.5%, 1,3- propane diols 1.0%, Phenoxyethanol 0.3%, methyl hydroxybenzoate 0.3%, ethyl hydroxy benzoate
0.2%th, Sensiva SC50 0.2%, parachlorometaxylenol 0.3%, lactic acid 0.05%, sodium lactate 0.05%, add deionization
Water is to 100.
1.3.22 unartificial yeast composition private portion's gel (part by weight) is matched
Match unartificial yeast composition 25.0%, borax 2.5%, carbomer 1.7%, glycerine 1.5%, Phenoxyethanol 0.3%,
Methyl hydroxybenzoate 0.3%, ethyl hydroxy benzoate 0.2%, Sensiva SC50 0.2%, add deionized water to 100.
2. Efficacy experiments
2.1 moisture retentions are tested
(man 5, female 15 by the men and women 20 without disease of skin that experimental selection can be influenceed by result of the test completely
Name) tested for object, with the moistening effect of test event unartificial yeast composition.Specific method is as follows：Each experimental subjects
Before sample, first with behind water cleaning experiment position, then external environment condition is adapted to 30 minutes.The environmental condition of experiment is：Keep indoor
22-24 DEG C of temperature, indoor humidity 45-50%, and tested in same place.Sample is smeared such as with 2*2cm2 in inboard arm
Each 10ul shown in table 1, and dry 1 minute.Then the ultra-pure water for spreading 20ul is respectively applied again at the position of smearing, with towel water
Point after with 1 point for interval (0 minute, 1 minute, 2 minutes, 3 minutes, 4 minutes, 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes,
10 minutes), moistening effect is measured to the skin surface moisture at each smearing position with Corneometer CM 825.Measurement
As a result as shown in table 2, unit is Corneometer value (Cv).
The each sample composition of table 1 composition situation is as follows：
Note："-" represents to be not added with.
The each sample moistening effect measurement result of table 2 is as follows：
| 0 point | 2 | 4 | 6 | 8 | 10 | |
| Sample 1 | 156.3 | 66.2 | 58.6 | 50.1 | 48.4 | 46.2 |
| Sample 2 | 160.6 | 70.2 | 60.5 | 52.2 | 49.1 | 47.5 |
| Sample 3 | 157.5 | 68.1 | 59.4 | 51.2 | 49.5 | 46.7 |
| Sample 4 | 163.7 | 74.3 | 63.0 | 54.0 | 50.2 | 48.7 |
| Sample 5 | 163.9 | 74.9 | 63.5 | 54.6 | 50.5 | 49.5 |
| Sample 6 | 165.4 | 76.8 | 65.9 | 56.4 | 51.4 | 50.8 |
| Sample 7 | 164.4 | 75.4 | 64.6 | 55.6 | 50.9 | 50.1 |
| Sample 8 | 167.6 | 79.8 | 67.5 | 57.2 | 52.2 | 51.5 |
| Sample 9 | 166.6 | 78.8 | 67.1 | 56.9 | 51.6 | 50.9 |
| Sample 10 | 168.0 | 80.2 | 67.9 | 57.8 | 53.7 | 52.6 |
| Sample 11 | 168.5 | 80.9 | 68.4 | 58.3 | 55.9 | 55.1 |
| Sample 12 | 172.6 | 72.5 | 62.8 | 56.2 | 52.7 | 51.3 |
| Sample 13 | 176.9 | 76.9 | 66.5 | 62.4 | 58.2 | 56.8 |
| Sample 14 | 181.6 | 82.4 | 72.0 | 65.8 | 61.3 | 60.1 |
| Sample 15 | 180.0 | 81.2 | 70.0 | 64.4 | 60.1 | 59.7 |
| Sample 16 | 186.2 | 85.8 | 73.5 | 67.2 | 63.5 | 62.9 |
| Sample 17 | 187.8 | 87.5 | 75.8 | 69.5 | 64.1 | 63.8 |
| Sample 18 | 190.2 | 92.3 | 78.1 | 72.4 | 68.1 | 67.4 |
| Control group 1 | 156.3 | 66.4 | 58.5 | 50.1 | 48.3 | 46.4 |
| Control group 2 | 144.2 | 60.5 | 51.3 | 43.6 | 42.0 | 40.3 |
| Control group 3 | 160.5 | 72.7 | 62.4 | 53.2 | 51.1 | 49.4 |
Conclusion：
(1) from table 1,2, unartificial yeast, plant regulation agent and powder raw material three are by certain weight ratio culture, hair
The match unartificial yeast composition obtained after ferment, processing is than single unartificial yeast, plant regulation agent, powder raw material solution moisturizing effect
Fruit is more preferable, illustrates that match unartificial yeast composition has obvious chemiluminescence in terms of moistening effect；
(2) from table 1,2, unartificial yeast of the mass fraction in the range of 0.5-5.0%, in 50.0-80.0% scopes
The match unartificial yeast that interior plant regulation agent, the powder raw material in the range of 1.5-5.0% obtain after cultivating, ferment, handle
Composition is respectively provided with chemiluminescence in terms of moistening effect；
(3) comparative sample understand, unartificial yeast, plant regulation agent and powder raw material three addition be 2.0%,
65.0%th, 3.0% when, moisturizing chemiluminescence is most reasonable, inadequate less than this value moistening effect, higher than this value moisturizing imitate
Fruit amplification slows down and the problems such as expecting body waste, cost increase occurs.
2.2 percutaneous absorbabilities are tested
The mouse skin cuticula handled well is fixed on Franz diffusion cells upwards according to Transdermal Absorption experiment, connect
Receives pond
Middle addition 30% ethanol-physiological saline, is placed in the intelligent penetrating absorption instrument of water bath with thermostatic control, design temperature (37.0
± 0.5) DEG C,
Rotating speed 200rmin-1.1h is balanced, sample is added after draining the bubble in reception tank.On diffusion cell supply area
Uniformly smear sample each 1g as shown in table 3, every group parallel 3 times, respectively 0,0.1,0.3,0.5,1.0,2.0,4.0,6.0,
8.0th, 10.0,12.0 when draw reception liquid 1mL, and fill into the reception liquid of equality of temperature same volume.The reception liquid of absorption adds after filtering
Enter test tube, then it is accurate add 2mLCTAB test solutions, the timing from when adding, gently vibrate and rock enough long-times, during which try one's best
Avoid reaction solution from producing foam, stand to close to 10min, then pour into reaction solution in 1cm cuvettes, determine ripple during 10min
The absorbance (A400) in 400nm is grown, the transdermal of hyaluronic acid is obtained according to the standard curve of hyaluronic acid at that wavelength
Absorptivity.
The each sample composition of table 3 composition situation is as follows:
Note："-" represents to be not added with.
Conclusion：Such as Fig. 1 percutaneous absorbability experimental result pictures, using hyaluronic acid transdermal absorption factor as ordinate, the time (t) is
Abscissa, hyaluronic acid transdermal absorption factor-time graph is drawn, sees Fig. 1, the absorptivity for as a result showing hyaluronic acid in 12h is
The ＞ control groups of one ＞ samples of sample, three ＞ samples two, show unartificial yeast, plant regulation agent and powder raw material three by certain weight
Measure than cultivate, ferment, handle after obtained match unartificial yeast composition be remarkably improved the transdermal absorption factor of hyaluronic acid；As a result
Show that absorption of the 0.5h inner skins to hyaluronic acid is most fast, the match unartificial yeast composition is in 0.5h to the transdermal of hyaluronic acid
Absorptivity influences most notable；Experiment shows that the match unartificial yeast composition has by the research to hyaluronic acid transdermal absorption factor
Effect is received in certain rush imbibition.
The compound prescription experiment of 2.3 research match unartificial yeast compositions
Now using human experimentation method detection match unartificial yeast composition human body bioactivity, i.e., it is calm releive,
Anti-inflammatory reparation, opsonic activity is nourished, it is uncomfortable with conditioning skin to prevent to study activity match unartificial yeast composition.
All experimenters of this experiment are different degrees of skin discomfort person, and each sample matches unartificial yeast composition components group
Into combination as shown in table 4, that experimental group 1 is prepared using unartificial yeast 2.0%+ plant regulation agent 65.0%+ powder raw materials 3.0%
Thing sample is smeared 1 time before sleeping every night；The composition sample that experimental group 2 is prepared using unartificial yeast 2.0%+ plant regulations agent 65.0%
Product are smeared 1 time before sleeping every night；Experimental group 3 uses the composition sample of the preparation of unartificial yeast 2.0%+ powder raw materials 3.0% every night
Smeared 1 time before sleeping；The composition sample that positive control is prepared using unartificial yeast 2.0% is smeared 1 time before sleeping every night；Negative control
Smeared 1 time before being slept every night using physiological saline；Symptom improves situation after comparing five groups of conditionings 7 days.
Each experimental group sample solution composition composition situation of table 4 is as follows:
| Unartificial yeast | Plant regulation agent | Powder raw material | Deionized water | |
| Experimental group 1 | 2.0% | 65.0% | 3.0% | To 100 |
| Experimental group 2 | 2.0 | 65.0% | - | To 100 |
| Experimental group 3 | 2.0 | - | 3.0% | To 100 |
| Positive controls | 2.0 | - | - | To 100 |
| Negative control group | - | - | - | Physiological saline is to 100 |
Note："-" represents to be not added with.
The symptom assessments such as dermal sensation discomfort, allergy, inflammation, microgroove, peculiar smell, spot：
It is effective：Symptom is significantly improved or disappeared, experimenter's satisfaction；Effectively：Symptom has improvement or mitigated, and experimenter still has not
It is suitable, it is desirable to continue to nurse one's health；It is invalid：Symptom mitigates without obvious, and experimenter is unsatisfied with.
Statistical method：Statistical procedures are carried out using SPSS12.0 statistics softwares, compared between metering and enumeration data group
Examined compared with being examined using t with x2, with p<0.05 is statistically significant difference.
As a result it is as follows：
As shown in table 5, the 8th day symptom improves situation：Experimental group 1 effective 10, total effective rate 100%；Experimental group 2 is effective
5, effective 2, invalid 3, total effective rate 70%, obvious effective rate P compared with experimental group 1<0.01；Experimental group 3 effective 3, has
Effect 3, invalid 4, total effective rate 60%, obvious effective rate P compared with experimental group 1<0.01；Effective 0 of positive control, effective 2,
Invalid 8, total effective rate 20%, obvious effective rate P compared with experimental group 1<0.01.
The 8th day each group experimenter symptom of table 5 improves situation and is compared as follows：
Conclusion：
Experimental group 1 (composition sample prepared by unartificial yeast 2.0%+ plant regulation agent 65.0%+ powder raw materials 3.0%)
Substantially than experimental group 2 (composition sample prepared by unartificial yeast 2.0%+ plant regulations agent 65.0%), (the natural ferment of experimental group 3
Composition sample prepared by female 2.0%+ powder raw materials 3.0%), positive control it is effective.Present invention match unartificial yeast composition is used
After human experimentation shows (1) unartificial yeast, plant regulation agent and powder raw material three by certain weight ratio culture, fermentation, processing
Obtained match unartificial yeast composition is to prevention and conditioning skin discomfort significant effect；(2) unartificial yeast, plant regulation agent and powder
Shape raw material three by the match unartificial yeast composition obtained after certain weight ratio culture, fermentation, processing in a short time calm releive,
Anti-inflammatory reparation, nourishing conditioning skin problem have certain effect, and permanence operation can be more preferably；(3) unartificial yeast, plant regulation agent
Prevented and nursed one's health by the match unartificial yeast composition obtained after certain weight ratio culture, fermentation, processing with powder raw material three
The uncomfortable aspect of skin has obvious chemiluminescence.
The optimum proportioning experiment of 2.4 research match unartificial yeast composition compound prescriptions
0.5%, 2.0%, 5.0% unartificial yeast, the plant of proportioning 50.0%, 65.0%, 80.0% will be matched respectively
Conditioner, the powder raw material of proportioning 1.5%, 3.0%, 5.0% are prepared into match unartificial yeast composition, and deionized water is mended to quality
Percentage summation 100%.All experimenters of this experiment are different degrees of skin discomfort person, experiment 1-27 with unartificial yeast,
The composition sample that plant regulation agent and powder raw material three are prepared by different ratio smears 2 three factors three of progress sooner or later daily
Horizontal orthogonal experiment, the situation after observation conditioning；Proportioning 0.5%, 2.0%, 5.0% is respectively adopted in positive control 28-30
Composition sample prepared by unartificial yeast is smeared 2 times sooner or later daily, the situation after observation conditioning；31 be that negative control uses physiology
Salt solution is smeared 2 times sooner or later daily, the situation after observation conditioning；Every group of 5 patients, comparing symptom after nursing one's health 7 days improves situation.
The orthogonal experiment factor meter of table 6：
The symptom assessment standards such as dermal sensation discomfort, allergy, inflammation, microgroove, peculiar smell, spot are same as above.
Statistical method：Statistical procedures are carried out using SPSS12.0 statistics softwares, compared between metering and enumeration data group
Examined compared with being examined using t with x2, with p<0.05 is statistically significant difference.
The 8th day each group experimenter symptom of table 7 improves situation and is compared as follows：
Conclusion：
Experiment is used through human body, the results showed that uses the unartificial yeast of proportioning 0.5%, 2.0%, 5.0%, proportioning
50.0%th, 65.0%, 80.0% plant regulation agent, match prepared by the powder raw material of proportioning 1.5%, 3.0%, 5.0% are natural
Yeast compositions it is calm releive, anti-inflammatory reparation, nourish conditioning skin problem in terms of have the effect of certain, but do not limit to this model
Enclose；When the unartificial yeast that proportioning is 2.0%, the plant regulation agent for 65.0% is matched, matches the powder raw material preparation for 3.0%
During into match unartificial yeast composition, effect embodiment is most obvious, and the proportioning is optimum proportioning.
2.5 bacteriostatic experiment
Take Clinical isolation Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans a little, connect respectively
Kind cultivates 18h in broth bouillon at 37 DEG C.(calf serum that 20% is added in Pseudomonas aeruginosa broth bouillon) takes
Each bacterial strain nutrient broth culture of 18h cultures, it is made into bacteria suspension and is used to test.Take sterilizing test tubes 11 respectively, the 1st
Nutrient broth fluid nutrient medium 9ml is added, 2-10 branch adds 5ml, the 11st addition 10ml, separately sampled product solution such as table 8
Shown 1ml adds the 1st test tube, takes 5ml to add the 2nd after well mixed, is diluted to the 10th successively, the 11st is not added with sample
As control.Often pipe adds escherichia coli suspension 0.1ml, and 24h is cultivated at a temperature of 37 DEG C, takes out observation bacterial growth situation.
Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans are the same as above-mentioned Germicidal efficacy growing state.As test tube becomes cloudy, that is, represent
Bacterial growth, sample is without bacteriostasis；As test tube is limpid, expression bacterial growth is suppressed.Experimental result is as shown in table 9, this
Invention match unartificial yeast composition is to Clinical isolation Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans
There is stronger bacteriostasis.
The bacteriostatic experiment each sample solution composition of table 8 forms
| Unartificial yeast | Plant regulation agent | Powder raw material | Deionized water | |
| Sample 1 | 2.0% | 65.0% | 3.0% | To 100 |
| Sample 2 | 2.0% | 65.0% | - | To 100 |
| Sample 3 | 2.0% | - | 3.0% | To 100 |
| Sample 4 | - | 65.0% | 3.0% | To 100 |
| Sample 5 | 2.0% | - | - | To 100 |
| Sample 6 | - | 65.0% | - | To 100 |
| Sample 7 | - | - | 3.0% | To 100 |
Note："-" represents to be not added with.
The each sample solution of table 9 corresponds to last limpid test tube numbering in bacteriostatic test
Note："-" represents not limpid.
By above-mentioned limpid broth tubes and control group the transferred species broth agar plates after diluting according to a certain percentage, observation
24h, the Cmin of no bacterial growth is bacteriocidal concentration, is designated as C, unit mg/mL, as a result as shown in table 10, illustrates this
Invention match unartificial yeast composition is to being clinically separated common Bacteria skin infection strain Escherichia coli, staphylococcus aureus, green pus
Bacillus, Candida albicans have stronger bactericidal action, can effectively suppress the activity of these Bacteria skin infections to prevent and adjust
It is uncomfortable to manage skin；Simultaneously by contrast as can be seen that unartificial yeast, plant regulation agent and powder raw material three press certain weight ratio
The match unartificial yeast composition obtained after culture, fermentation, processing also has chemiluminescence at antibacterial aspect.
The each sample solution sterilization conditions of table 10
| C (Escherichia coli) | C (staphylococcus aureus) | C (Pseudomonas aeruginosa) | C (Candida albicans) | |
| Sample 1 | 0.12 | 0.15 | 0.17 | 0.11 |
| Sample 2 | 0.22 | 0.37 | 0.40 | 0.25 |
| Sample 3 | 0.76 | 0.50 | 0.80 | 0.66 |
| Sample 4 | 0.47 | 0.52 | 0.55 | 0.60 |
| Sample 5 | - | - | - | - |
| Sample 6 | 0.43 | 0.51 | 0.75 | 0.52 |
| Sample 7 | 0.73 | 0.84 | 0.81 | 0.92 |
| Control group | - | - | - | - |
Note："-" indicates no bactericidal properties.
2.6 acute oral toxicities are tested
According to test basis《Disinfection technology standard》(defend method prison hair in the 3.4 of (third edition) first fascicle experimental technique specification
[1999] No. 448).By animal (regular grade NIH mouse 20, male and female half and half, 18~22g of body weight) water supply fasting 12 hours,
Distilled water is solvent, tested material (the match day that unartificial yeast 2.0%, plant regulation agent 65.0%, powder raw material 3.0% are prepared into
Right yeast compositions) dosage is set as 5500mg/kg, tested material is once filled to animal with gavage syringe needle by 0.2ml/20g body weight.
Observed immediately after administration and record the poisoning manifestations, death toll and death time of animal, observe secondary, two weeks observation periods daily.
Toxicity grading is carried out according to acute toxicity grading criteria.Experimental result：In observation period, test mice does not occur manifest symptom, nothing
Death occurs.
The tested material of table 11 is to NIH chmice acute Oral toxicity result of the tests
| Sex | Dosage (mg/kg) | Number of animals (only) | Death toll (only) | The death rate (%) |
| It is female | 5500 | 10 | 0 | 0 |
| It is male | 5500 | 10 | 0 | 0 |
Conclusion：Present invention match unartificial yeast composition to the acute through LD50 ＞ 5000mg/kg of NIH mouse, true border without
Malicious level.
2.7 vaginal mucosa stimulation tests
Press《Disinfection technology standard》(third edition) first fascicle《Experimental technique specification》The 3.5th in (Ministry of Public Health, 2012.4)
Carry out.Healthy White Rabbit 28 is chosen, is divided into experimental group 24, excipient group 2 and blank control group 2.Experimental group：It will apply
The roundlet sliver for having the 2.0cm length of sample 1-12 test specimens inserts experimental animal intravaginal, every kind of each 2 of sample；Excipient
Group：Soaked with an equal amount of sliver with sterile saline, insert animal intravaginal；Blank group：Do not process.Removed after 4 hours
Sliver is removed, animal is put to death after 24 hours, takes out topical vaginal tissue, phenomena such as whetheing there is hyperemia, oedema observed.
The vaginal mucosa stimulation test each sample composition of table 12 forms
The vaginal mucosa stimulation test result of table 13 is as follows：
| Symptom of erythema | Edematous condition | Stimulate the reaction integrates | |
| Sample 1 | - | - | 0 |
| Sample 2 | - | - | 0 |
| Sample 3 | - | - | 0 |
| Sample 4 | - | - | 0 |
| Sample 5 | - | - | 0 |
| Sample 6 | - | 1 | 1 |
| Sample 7 | - | - | 0 |
| Sample 8 | - | - | - |
| Sample 9 | 1 | - | 1 |
| Sample 10 | - | - | 0 |
| Sample 11 | - | - | 0 |
| Sample 12 | 2 | 1 | 3 |
| Excipient group | - | - | 0 |
| Blank group | - | - | 0 |
Note："-" indicates no erythema or edematous condition；" 0 " represents no vaginal irritation reaction.
In observation period, test group sample 1-5, sample 7-8, sample 10-11, excipient group, blank control group animal are local
Vagina tissue does not occur erythema or edematous condition, and stimulate the reaction integral mean value is 0, according to《Disinfection technology standard》(third edition)
One fascicle《Experimental technique specification》Relevant criterion judges in (Ministry of Public Health, 2012.4), and these samples stick to healthy White Rabbit vagina
Film is nonirritant；And the appearance erythema or oedema that test group sample 6, sample 9, the animal topical vaginal tissue of sample 12 are different degrees of
Symptom, stimulate the reaction integration are not 0, according to《Disinfection technology standard》(third edition) first fascicle《Experimental technique specification》(health
Portion, 2012.4) relevant criterion judges in, and these samples have different degrees of excitant to healthy White Rabbit vaginal mucosa.
As a result show, the match unartificial yeast composition of high content plant regulation agent can make vagina tissue erythema or oedema occur
Symptom, excitant can be produced to vaginal mucosa, excitant is obvious when plant regulation agent content reaches 80.0%；It can also be seen that
The unartificial yeast of certain content and powder raw material can effectively alleviate vagina tissue erythema caused by the plant regulation agent of high concentration,
The side effects such as oedema, avoid irritating generation；Each composition in the unartificial yeast composition of present invention match simultaneously is gone back after processing
Reparation can be cooperateed with to nourish vagina and be damaged mucous membrane, strengthen vaginal mucosa immunity, contain RTI and inflammatory reaction, maintain
Body normal health.
Embodiments of the invention are the foregoing is only, not thereby limit the scope of patent protection of the present invention, every utilization
The equivalent structure or equivalent flow conversion that description of the invention and accompanying drawing content are made, or directly or indirectly it is used in other correlations
Technical field, be included within the scope of the present invention.
Claims (10)
1. one kind match unartificial yeast composition, said composition include following weight than component：
Wherein, unartificial yeast is for active dry yeasr, yeast content and yeast product, active dry yeasr through cultivating, fermenting, handle
The barms not digested after activation；Yeast content is that yeast wherein protein, nucleic acid, vitamin etc. will be taken out after broken wall
Carry, then the amino acid rich in small molecule through biological enzymolysis, peptide, nucleotides, vitamin, mineral matter natural active matter, wherein
Amino acid accounts for yeast content content more than 30%, and total protein accounts for yeast content more than 50%, and nucleotides accounts for yeast content
More than 10%；Yeast product is active dry yeasr decomposition nutrition matter, including ethanol, carbon dioxide, lactic acid；
Also, the weight ratio of active dry yeasr, yeast content and yeast product is 1-2 in unartificial yeast:20-30:5-8, institute
It is 0.5-5.0% to state unartificial yeast addition in composition.
2. match unartificial yeast composition as claimed in claim 1, it is characterised in that the unartificial yeast is that natural plants are cultivated
Fermented bacterium, the strain are Lu Shi Zygosaccharomyceses CGMCC2.1915 (numbering of corresponding strain, the adopted name of strain.), youth
Must can any of Candida CGMCC2.1763, saccharomyces cerevisiae CGMCC2.1 and phaffiafhodozyma ATCC66270, it is every kind of
Strain addition is 0.5-5.0%.
3. match unartificial yeast composition as claimed in claim 1, it is characterised in that plant regulation agent is by white fungus, gardenia, dragon
Blood tree, pseudo-ginseng, motherwort, sucrose composition, and mass ratio of each component is 3-5:1-3:1-2:1-2:2-3:1-3.
4. match unartificial yeast composition as claimed in claim 3, it is characterised in that white fungus, Cape jasmine in the plant regulation agent
Sub- flower, dragon tree, pseudo-ginseng, motherwort, sucrose optimum weight ratio 3.5:1.5:1:1:2:1.
5. match unartificial yeast composition as claimed in claim 1, it is characterised in that the natural powder raw material by glucose,
Hyaluronic acid, polyglutamic acid, collagen, vitamin C composition, and mass ratio of each component is 10-15:1-2:1-3:1-2:3-
5。
6. match unartificial yeast composition as claimed in claim 5, it is characterised in that the grape in the natural powder raw material
Sugar, hyaluronic acid, polyglutamic acid, collagen, vitamin C press optimum quality ratio 12:1:1.5:1:3.5.
7. match unartificial yeast preparation method of composition, it is characterised in that preparation process includes：
A, the Lu Shi Zygosaccharomyceses after the preferred activation of unartificial yeast strain, youth must can Candida, saccharomyces cerevisiae and red hair husband's ferment
Any of mother, save backup；
B, white fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose 3-5 in mass ratio:1-3:1-2:1-2:2-3:1-3 groups
Into plant regulation agent, co-grinding adds 10 times of amount water into 1000 grams of coarse powder, boiling 2 hours, filtration treatment, and filter residue adds 8 times of amounts
Water, boiling 1 hour, filtration treatment；Filter residue adds the water of 8 times of amounts again, and boiling 1 hour, filtration treatment, filtrate merges, at 50 DEG C
Be concentrated under reduced pressure into the clear cream that relative density is 1.0g/ml, in use, plus ethanol make alcohol content up to 60%, stand 24 hours, mistake
Filter, decompression filtrate recycling ethanol is simultaneously concentrated into no alcohol taste, standby after sterilizing；
C, glucose, hyaluronic acid, polyglutamic acid, collagen, vitamin C 10-15 in mass ratio:1-2:1-3:1-2:3-
The natural powder raw material of 5 compositions is simultaneously well mixed, standby after sterilizing；
D, above-mentioned standby barms is taken to use day under the conditions of constant temperature (25~30 DEG C), constant humidity (50~57%), pH (4.0-7.0)
Sucrose solution shaking table culture or the fermentation tank aerobic culture of right plant regulation agent obtain unartificial yeast stoste in 1-2 days, liquid
Unartificial yeast is stirred for 25~30 DEG C with powder raw material low temperature, static to place 1-4 days, surveys pH5.0-5.5, terminates fermentation, obtains
To yeast fermenting mixture；
E, above-mentioned yeast fermenting mixture is taken, adds 0.5g1:1-1:The pectase and extraction from yeast enzyme of 3 mixing, in 50-60 DEG C
2-8h is digested, 100 DEG C of stabilization processes 10min, 2000-3000RPM centrifugations, takes supernatant with after 0.22 μm of filtering with microporous membrane
PH5.0-5.5 is adjusted, unartificial yeast composition is as matched, saves backup.
8. match unartificial yeast preparation method of composition as claimed in claim 7, it is characterised in that the above method also includes：
F, the match unartificial yeast composition is slowly added into the formulation configured in advance with matrix auxiliary material, being gently mixed makes
It is completely dispersed；
G, pH4.0-9.0 is adjusted, it is standby that corresponding dosage form product is made.
9. according to the preparation method described in claim 7, it is characterised in that the Shandong after the preferred activation of the unartificial yeast strain
Family name's Zygosaccharomyces CGMCC2.1915, youth must can Candida CGMCC2.1763, saccharomyces cerevisiae CGMCC2.1 and phaffiafhodozymas
Any of ATCC66270；White fungus, gardenia, dragon tree, pseudo-ginseng, motherwort, sucrose in the plant regulation agent is optimal
Weight is than 3.5:1.5:1:1:2:1；Glucose, hyaluronic acid, polyglutamic acid, collagen in the natural powder raw material,
Vitamin C presses optimum quality ratio 12:1:1.5:1:3.5.
10. application of any described match unartificial yeast compositions of claim 1-6 in cosmetics are prepared.
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