CN107496195A

CN107496195A - A kind of recombined human hyaluronic acid compositions and its application in cosmetics

Info

Publication number: CN107496195A
Application number: CN201710648013.3A
Authority: CN
Inventors: 贺晓军; 侯如标; 叶轶; 李秋艳
Original assignee: Hangzhou Dr Hui Health Industry Co Ltd
Current assignee: Hangzhou Dr Hui Health Industry Co Ltd
Priority date: 2017-08-01
Filing date: 2017-08-01
Publication date: 2017-12-22

Abstract

The present invention provides a kind of recombined human hyaluronic acid compositions, and said composition includes weight ratio for 0.001% 9.0% recombined human hyaluronic acids, 0.2% 20.0% plant compounds, 0.05% 10.0% skin conditioning agents, 61.0% 99.7% matrix auxiliary materials；Described recombined human hyaluronic acid is cut macromolecular hyaluronic acid by the recombined human hyaluronidase PH20 for having saccharification produced using Chinese hamster ovary celI and obtained, and the recombined human hyaluronic acid at least one end can be identified the end of the recombined human hyaluronic acid cleavage of hyaluronic acid normal configuration by use.The cosmetics prepared with recombined human hyaluronic acid compositions, can not only promote mucocutaneous secretion functional component；And make it that active principle quickly spreads and infiltrates into cell interior in cosmetics.

Description

A kind of recombined human hyaluronic acid compositions and its application in cosmetics

Technical field

The present invention relates to filed of daily-use chemical industry, more particularly to a kind of recombined human hyaluronic acid compositions and its in cosmetics Application.

Background technology

At present, increasing people begins to use various cosmetics to the face of oneself, oral cavity, hair, body And privates etc. is cleaned, maintained, beauty, modification and changes outward appearance, but the daily chemical product of in the market is a feast for the eyes, class Uneven, this allows consumer to be difficult choice.With the development and the improvement of people's living standards of fine chemical technology, Ren Mengeng Tend to the daily chemical product that safety coefficient is higher, stability is more longlasting, practicality is stronger.

Hyaluronic acid, also known as Hyaluronic Acid, it is a kind of high molecular polymer, by unit D-Glucose aldehydic acid and N- acetyl Portugal The advanced acid mucopolysaccharide of osamine composition, it is the tender important foundation material of skin water, itself is also a kind of composition of human body, it With special water retention, deal is more up to 100 times of itself weight, is that moisture retention is best in the nature having now been found that Material, it can also improve skin-nourishing metabolism, make skin it is tender, it is smooth, go to wrinkle, increase it is elastic, prevent aging, in moisturizing While be good skin penetration enhancer again, be used cooperatively with other nutritional ingredients, can play and promote nutrient absorption More preferable effect.In the source of existing hyaluronic acid, the method that animal is extracted is because raw material is limited, and hyaluronic acid in raw material Content is low, causes low yield, purification hardly possible, cost is high, product quality is poor, it is difficult to large-scale production；The side of biofermentation extraction Technique fermentation period that method uses is often longer, product relative molecular weight is low, product protein content is high, glucuronic acid content It is low, product light transmittance is poor, some even present threadiness form, not readily dissolve, also can not be applied to cosmetics production； The hyaluronic acid that the method for the hyaluronic acid ferment treatment general macromolecular hyaluronic acid of animal arrangement obtains does not pass through recombined human The process of hyaluronic acid ferment treatment, lose the activity of recombined human hyaluronic acid.

The normal transparent matter acid effect added in existing components of cosmetics is single.Mucocutaneous secretion work(can not only be promoted Imitate composition, it is difficult to promote collagen and elastomer to recover original smooth and elastic；And it is difficult to so that components of cosmetics Central active principle infiltrates into cell and accelerates to spread and absorb, and does not play the action and efficacy of active principle in cosmetics.

The content of the invention

To solve technical problem existing for the normal transparent matter acid added in existing cosmetics, the present invention is realized in 's：

A kind of recombined human hyaluronic acid compositions, said composition include following weight than component：

Described recombined human hyaluronic acid is cut by the recombined human hyaluronidase PH20 for having saccharification produced using Chinese hamster ovary celI The acquisition of macromolecular hyaluronic acid is cut, and the recombined human hyaluronic acid at least one end can identify that hyaluronic acid is normally tied by use The end of the recombined human hyaluronic acid cleavage of structure.

The present invention uses recombined human hyaluronic acid not using normal transparent matter acid.Pass through recombined human hyaluronidase The recombined human hyaluronic acid for being digested to obtain to normal transparent matter acid has very strong bioactivity.Recombined human hyaluronic acid is not But there is the effect of normal transparent matter acid, mucocutaneous secretion functional component can also be promoted, and then suppress in inflammation and antagonism The inflammatory stimulus effect of toxin, while recombined human hyaluronic acid can make the direct rapid osmotic of active principle to cell and accelerate to expand Dissipate and absorb, there is efficient osmotic absorption, the functioning efficiency of active principle can be increased, when recombined human hyaluronic acid enters After skin, collagen and elastomer can also be made to recover original smooth and elastic, repair skin and mucosa protection barrier, And then play the effect of deep layer is repaired.Coordinate plant compound, skin conditioning agent, active ingredient in cosmetics can be effectively facilitated Absorption, improve the action and efficacies of cosmetics.

After the active ingredient of plant compound is concentrated, it can efficiently suppress or kill Candida albicans, golden yellow Portugal The bacteriums such as grape coccus, Escherichia coli, Pseudomonas aeruginosa and fungi, so as to reduce as the skin and mucosa infection and inflammation caused by them The generation of reaction；The active ingredient in plant compound can also promote skin and mucosa to secrete functional component simultaneously, and then suppress The inflammatory stimulus of inflammation and anti-endotoxin act on, and reach the effect of enhancing skin and mucosa immunity；Appropriate plant compound Skin surface and the internal inflammation secretion and toxin stockpiled can also be excluded by adding, and recover the self-cleaning self-purification capacity of skin, be maintained The living environment of skin normal flora, lasting deep layer nourishes rough skin mucous membrane, so as to reduce the generation of all kinds of disease of skin.

Each composition in skin conditioning agent after certain proportion and method complex treatment obtain, can help skin anti-inflammatory, Antiallergy, releive and promote cell repair；Ingredient stability is good simultaneously, and skin has good compatibility, reduces scytitis, Mitigate acne, prevent dark sore from producing, improve the anti-irritant ability of skin；Contain abundant required micro member in conditioner Element, mineral matter and component, these materials are all the necessary elements of human body, can deep layer skin is nursed one's health, maintain skin most Good health status.

Matrix auxiliary material can select deionized water, NMF, thickener, emulsifying agent, surfactant, functional component, anti- It is more than one or both of rotten agent, essence pigment, the figuration for the class product of face, oral cavity, hair, body and privates etc. five And other effects.

Preferably, the molecular weight of described recombined human hyaluronic acid is 0.7KD-100KD.

The molecular weight of hyaluronic acid active ingredient in the scope, cosmetics can be preferably absorbed by the skin.

Preferably, described plant compound extracts gained from active plant, and described active plant comprises at least reed Luxuriant growth silk slides element, dragon tree, strawberry fruit, resurrection grass, frankincense, motherwort, purslane, horse chestnut.

Aloe silk slides element：In nourishing and moisturizing aloe and complex polysaccharide material constitutes natural moisture preserving factor (NMF), and it can be with The moisture that loses in supplement skin, recover the function of collagen, prevent facial wrinkles, keep skin it is soft and moist, it is smooth, rich in bullet Property.It contains the composition such as histamine and prostaglandin inhibitor, ferric lactate, B family vitamin and enzyme, can prevent skin inflammation It is quick, bacterial growth, promote cell metabolism and skin regeneration, mitigate pain and itch, to pimples, whelk, allergy, redness It is effective in cure；Contained suppression melanin product, can make whiteness of skin, can also effectively go in whitening, nti-freckle aloe Except acne, whelk, chloasma and freckle, make modern female spring eternal beautiful.

Dragon tree：Dragon tree extract contains thaspine (Taspine) and former cyanine (PAC).Thaspine has very Strong inoxidizability, skin keratin barrier can also be strengthened, and repair the facial obscure, colour of skin caused by aging and external injury Uneven skin, significantly improves the colour of skin and skin pattern；OPC is that internationally recognized removing human free radical most has at present The natural of effect.It potent can resist inflammation and repair damage, while establish skin moisture barrier, improve red blood silk And sensitivity.

Strawberry berry extract：Contain aspartic acid in Fragaia ananassa Duchesne extract, the internal weight gold of removing that can be naturally gentle Belong to ion, in terms of beauty, in addition to it can improve dim heavy, the gentle whitening colour of skin containing left-handed C, and flavonoids and the tan flower contained The compositions such as acid, skin ageing can be prevented and prevent the formation of spot, rich in strawberry polyphenol there is anti-oxidant, reinforcement skin to become clear Effect, in addition, the natural tartaric acid in strawberry extract, also there is purification pore and cutin-softening to reduce the work(for the pore that compacts Effect, can make skin soft and more gloss, give skin it is absolute moisten and care, be commonly used to be added to oxidation and removing free radicals and In anti-ageing shining face series.

Bring back to life careless extract：Botanist goes Jordan desert, decodes the magical grass that brings back to life and meets the water storage power that water is lived again, and rises The maintenance power made waterpower, gather natural mineral water of level injection various plants essence synthesis of natural moisturizing factor, depth maintenance are protected Wet barrier, water circulation in a steady stream is formed, it is close to be familiar with natural aquaporin flesh, solves percutaneous absorption problem from root, remolds flesh bottom moisturizing Function, the water shortage crisis of dry skin is saved, the rapid full water of skin, which is assigned, lives, and presents vigorous.

Frankincense：Olibanum extract is the Hydrolysis kinetics from the gum resin of India traditional plant Boswellia carterii, remains work Property most strong composition B- masticinic acids, there is unique dual inhibition of inflammation, two kinds of proinflammatory reaction enzymes can be suppressed simultaneously, 5- Lipoxidase (leukotrienes inflammatory factor can be produced) and human leukocyte elastase HLE.Frankincense is that ancient Gingko thousand is rare It traditional anti-inflammatory, nourishing beauty sage's product, can effectively alleviate livid ring around eye and pouch symptom, reduce eye microgroove, be traditionally used for always In crease-resistant formula and eye cream.

Leonurus extract has good facilitation to NMF generation, has moisture-keeping function to skin；To B- 16 cells have good inhibiting rate, therefore available for preventing and treating freckle, cutaneous pigmentation；With inoxidizability, with reference to paddy Guang The activation of sweet fabk polypeptide, it is believed that its is anti-ageing.

Purslane extract is mainly used in antiallergy, anti-inflammatory anti-inflammatory and the various stimulations for resisting outer bound pair skin, also has Acne-removal function, with nutrition greasy skin and epithelium can be promoted thin mainly due to plant polyose and vitamin is contained in purslane extract The physiological function of born of the same parents tends to be normal, reduces the generation of dead skin and cuticula caused by drying, amino acid can be smooth with vasoconstriction Flesh, maincenter that contraction has concurrently and peripheral, the skin and suppressing of can releiving dry caused by skin itching, flavones and saponin Free radical and anti-oxidant, delay skin aging can be removed, alkaloid and flavones can be true with antibacterial and suppression Common Pathogenic skin Bacterium.

Horse chestnut：Plant origin, purity is up to more than 90%, its mechanism of action：Horse chestnut can protect arteries and structure Completely, improve vein function, while take into account anti-inflammatory and oxidation etc. and play multi-efficiency；With further spreading out for research, seven leaves Tree suppresses Collagenase, hyaluronidase, B- glucuronidases and the pancreatopeptidase E (integrality of meeting catalytic destruction venous structures With oozing out for stingy capillary) effect be also proved)；It can be played in external prescription and stimulate circulation, promote wound healing Conjunction, antioedematous, anti-inflammatory are impervious to be gone out with vascular protection to act on (go livid ring around eye, go red blood silk, crease-resistant, maintenance).Aescin is seven leaves The most important composition to reduce inflammation in fruit is set, it is by filling up veinlet and capillary microscopic nicks and trickle visible Gap, the integrality of blood vessel astillen is can effectively improve, therefore aescin helps to repair capillary.

The extracting method of plant compound：Element, dragon tree, strawberry fruit, resurrection grass, frankincense, motherwort, horse are slided by aloe silk The eight kinds of active plant mixing of bitterroot and horse chestnut, clear cream is condensed into, then extracts gained.In the active plant compound combined formulation Other active ingredients can with efficient moisture-retention water lock, regulation skin water and oil balance, calmness is releived, anti-inflammatory is antibacterial, bright color, anti- Oxidation, anti-ageing wait for a long time have the function that deep layer nurses one's health skin.

Preferably, described skin conditioning agent comprises at least collagen, polyglutamic acid, allantoin, malt polysaccharide, natural Mineral water.

Collagen：Efficient moisture-retention composition, skin-active, inducing tissue regeneration, the main bonding material in human connective tissue, Molecular weight is big, moisture-retaining capacity compared with polyalcohols more preferably, mainly to support cell, especially suitable for after skin exfoliating, skin The physiotherapy made in the most thin stage is protected.Collagen has good permeability and moisturizing power, is mainly used in more scar, anti- Wrinkle, the conditioner of pachylosis patient.Collagen and its hydrolysate compatibility are strong, can coordinate with many functional components to carry It is high they the effect of, use can effective hair conditioning in hair product.

Polyglutamic acid：It is reported that polyglutamic acid (γ-PGA) is a kind of water-soluble, biodegradable, use without toxicity Boiomacromolecule made from microbe fermentation method, it is a kind of sticking material, is found first in " natto ".γ-PGA It is a kind of special anion nature polymer, is using the glutamic acid of left and right optical activity as cell cube, in alpha-amido and γ-carboxylic Formed homotype polyamide polypeptide is bonded between base through acid amides, γ-PGA molecular weight is from 5 ten thousand to 2 megadaltons. Special molecular structure, extremely strong moisture-retaining capacity is made it have, can effectively increase the moisture-retaining capacity of skin, promote skin to be good for Health, its superpower moisture-retaining capacity are better than common moisturizing ingredient, for the biotechnology NMF of a new generation.While γ-PGA are also With superpower adsorptivity and biodegradability, catabolite is non-harmful glutamic acid, is that a kind of excellent environment-friendly type is high Molecular material, water-loss reducer, adsorbent for heavy metal, flocculant, sustained release agent and pharmaceutical carrier etc. can be used as, cosmetics, The industries such as food, medicine have very big commercial value and social value.

Allantoin：It is main to do skin conditioning agent, protective agent use, it is good NMF, allantoin can reduce cuticula The adhesion strength of cell, keratin can be softened, accelerate epidermal cell renewal, additionally it is possible to helped skin anti-inflammatory, releive and promote thin Born of the same parents repair, and can mitigate stimulation, while have antioxidation.

Malt polysaccharide：It is colorless viscous shape liquid, is called Fructus Hordei Germinatus oligose base glucosides hydrogenated starch hydrolysates, is by starch The carbohydrate syrup derived, containing various carbohydrates, it is added to the moisture absorption to optimizing skin-protection product in skin nursing products Balance can keep the pliability of skin with unique booster action, improve the quality and smoothness of product, for it is non-stimulated into Point, it is especially suitable for being added in the formulation product for aiming at sensitive skin design, stimulation can be alleviated.

Skin conditioning agent：By collagen, polyglutamic acid, allantoin, malt polysaccharide and natural mineral water according to certain ratio Example and method collocation use, and not only with certain collaboration moisture retention, with deep layer reparation and skin can be nourished, to skin and skin Skin mucous membrane is effectively nursed one's health.

Further, described natural mineral water is selected from Alps glacial water, Changbai Mountain natural mineral water, Russia It is more than one or both of Caucasus Mountain mineral water, A Lewaer thermal waters, Canadian ooze mineral water.

It is required micro- containing abundant metasilicic acid, strontium, radon, zinc, fluorine, iodine, selenium, manganese, silicon, copper, calcium, magnesium etc. in natural mineral water Secondary element, mineral matter and component, these materials are all the necessary elements of human body, can deep layer skin is nursed one's health, maintain flesh The optimal health status of skin.

Preferably, described matrix auxiliary material is facial-care class cosmetic base auxiliary material, the facial-care class cosmetics base Matter auxiliary material is selected from deionized water, glycerine, butanediol, glycine betaine, glycerin polyether -26, dipotassium glycyrrhizinate, avenabeta glucosan, right Hydroxy acetophenone, 1,2- hexylene glycols, Cremophor RH40, potassium hydroxide, EDTA- disodiums, pentanediol, polyethylene glycol- 400th, D-sorbite, carbomer, hydroxyethyl cellulose, lipoic acid, hydrogenated olive oil, triethanolamine, essence, the different nonyl of different n-nonanoic acid Ester, saualane, dimethyl silicone polymer, MONTANOV L, SIMULGEL EG, niacinamide, tocopherol, bisabolol, acetyl shell Osamine, myristic acid, palmitic acid, stearic acid, potassium hydroxide, LC, laurate, methyl hydroxybenzoate polyethylene glycol -14M, melon glue hydroxyl Hydroxypropyltrimonium chloride, D-pantothenyl aleohol, sodium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, Cosmocil CQ, PLURONIC It is more than one or both of L64,0.05%GREEN 6, isomery hexadecane, ring five dimethyl silicone polymer.

Preferably, described matrix auxiliary material is oral care class cosmetic base auxiliary material, the oral care class cosmetics base Matter auxiliary material is selected from deionized water, glycerine, sorb (sugar) alcohol, honey powder, peppermint, potassium sorbate, sodium benzoate, ascorbic acid, the moon Cinnamic alcohol sodium sulfovinate, hydrated silica, xylitol, polyethylene glycol 400, chitosan, hydroxyethyl cellulose, sodium alginate, peppermint, sugar Smart sodium, microcrystalline cellulose, stevioside, sodium lauroyl glutamate, sodium acid carbonate, ascorbic acid, radix glycyrrhizae, magnesium stearate, silica, It is more than one or both of card ripple, tea extract, acesulfame potassium, propane diols, Tween-80, triethanolamine, sodium lactate.

Preferably, described matrix auxiliary material is that hair washes shield class cosmetic base auxiliary material, and the hair washes shield class cosmetics base Matter auxiliary material is selected from deionized water, glycerine, F-68, Finasteride, K220, AES, CAB, 6501, pearly-lustre dibasic acid esters, silicone emulsion, melon That glue C-14-S, citric acid, sodium chloride, shredding, essence, EDTA- disodiums, aloe extract, octadecyl alcolol, cation surface activating Agent, lanolin, sodium benzoate, phenmethylol, pigment, peregal, A25, EP-200, cera alba, methyl esters, propyl ester, lanolin, the third two It is more than one or both of alcohol, VA64.

Preferably, described matrix auxiliary material is body care class cosmetic base auxiliary material, the body care class cosmetics base Matter auxiliary material be selected from deionized water, 1,2- pentanediols, avenabeta glucosan, butanediol, trehalose, parahydroxyacet-ophenone, 1,2- oneself Glycol, dipotassium glycyrrhizinate, allantoin, Cremophor RH40, panthenol, essence, triethanolamine, methyl cocoyl taurine Sodium, LAA, sodium lauroyl sarcosine, coconut oleoyl amine methyl MEA, decyl glyceryl monooleate, polyglycereol- 10 oleates, caprylic/capric glyceride type Natrulon H-10 esters, PEG-120 methyls dioleate, cocounut oil acyl hydroxyl second sulphur Acid esters sodium, lauryl glucoside, MS621, Polyquaternium-10, sodium citrate, EDETATE SODIUM, glycerol monolaurate, citric acid, Dehydroactic acid, phenmethylol, caprylic/capric glyceride, shea butter, white oil, cetostearyl alcohol, silicone emulsion, AVC, different n-nonanoic acid Different nonyl ester, stearine, polysorbate -60, stereth -21, tocopherol, olive oil, cyclopentasiloxane, lecithin Fat, Phenoxyethanol, Sensiva SC50, stearic acid, hexadecanol, lanolin, Tween-80, private disk -60, K12, glycerine, salicylic acid One kind in phenyl ester, titanium dioxide, COCOAMIDOPROPYL BETAINE, DOW, sodium chloride, pigment, F-68, ketoconazole, K220 or It is two or more.

Preferably, described matrix auxiliary material is that shield class articles for use matrix auxiliary material is washed in private portion, and it is auxiliary that shield class articles for use matrix is washed in the private portion Material is selected from deionized water, glycerine, boric acid, Phenoxyethanol, methyl hydroxybenzoate, ethyl hydroxy benzoate, Sensiva SC50, Hamposyl L Sodium, SP-115B, LAA, 1,3- propane diols, parachlorometaxylenol, lactic acid, sodium lactate, borax, card ripple It is more than one or both of nurse, Nonoxynol-9, D-sorbite, sodium chloride, potassium hydroxide.

The beneficial effects of the present invention are：

The cosmetics containing recombined human hyaluronic acid, plant compound and skin conditioning agent composition of the invention, face, Oral cavity, hair, body and privates etc. application are above the daily use chemicals that safety coefficient is higher, stability is more longlasting, practicality is stronger Articles for use.Facial-care class cosmetics containing recombined human hyaluronic acid compositions, there is moisturizing, moisturizing, anti-inflammatory, antibacterial, anti-mistake The effect of quick, anti-oxidant, anti-acne, whitening, smoothing wrinkle；Oral care class cosmetics containing recombined human hyaluronic acid compositions, have Fresh breath, cleaning tooth, prevent, in protection and brilliant white tooth, also preventing and treating gingival sensitivity, canker sore, gingivitis and periodontitis Deng oral inflammation problem, also have and eliminate redness, prevent the oral problems such as bleeding gums；Contain recombined human hyaluronic acid compositions Hair wash shield class cosmetics and have and repair hair scale piece, nourish the effect of tough hair quality, regulation scalp grease；It is saturating containing recombined human The body care class cosmetics of bright matter acid composition, there are regulation body skin grease balance, moisturizing, whitening, bacteriostasis antibiosis, promotion new old Metabolism and other effects；Shield class articles for use are washed in private portion containing recombined human hyaluronic acid compositions, are had cleaning privates, are removed peculiar smell, maintain Privates normal flora balance, suppress to kill pathogenic bacteria, persistently moisten conditioning privates, the generation for reducing privates inflammation and other effects.

Brief description of the drawings

Fig. 1 is percutaneous absorbability experimental result picture.

Embodiment

To describe the technology contents of the present invention, construction feature, the objects and the effects in detail, below in conjunction with embodiment And accompanying drawing is coordinated to be explained in detail.

Embodiment 1

Facial-care class formula

(1) recombined human hyaluronic acid compositions solution (part by weight)

Recombined human hyaluronic acid 1.0%, plant compound 10.0%, skin conditioning agent 5.0%, add deionized water to 100.

(2) recombined human hyaluronic acid compositions conditioning spraying (part by weight)

Recombined human hyaluronic acid 0.1%, plant compound 0.5%, skin conditioning agent 10.0%, glycine betaine 1.0%, glycerine Polyethers -26 2.0%, dipotassium glycyrrhizinate 0.1%, avenabeta glucosan 0.2%, parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%th, Cremophor RH40 0.1%, potassium hydroxide 0.015%, EDTA- disodiums 0.05%, add deionized water to 100.

(3) recombined human hyaluronic acid compositions facial mask (part by weight)

Recombined human hyaluronic acid 0.5%, plant compound 5.0%, skin conditioning agent 8.0%, glycerine 5.0%, butanediol 3.0%th, pentanediol 1.5%, PEG-4000 1.0%, glycerin polyether -26 1.0%, D-sorbite 0.2%, carbomer 0.15%th, hydroxyethyl cellulose 0.08%, lipoic acid 0.01%, dipotassium glycyrrhizinate 0.1%, hydrogenated olive oil 0.1%, to hydroxyl Acetophenone 0.3%, 1,2- hexylene glycols 0.3%, Cremophor RH40 0.1%, triethanolamine 0.14%, EDTA- disodiums 0.05%th, essence 0.01%, deionized water is added to 100.

(4) recombined human hyaluronic acid compositions emulsion (part by weight)

Recombined human hyaluronic acid 0.01%, plant compound 2.0%, skin conditioning agent 5.0%, glycerine 5.0%, butanediol 3.0%th, isononyl isononanoate 4.0%, saualane 2.0%, glycerin polyether -26 1.0%, dimethyl silicone polymer 1.0%, MONTANOV L0.6%, SIMULGEL EG 0.5%, niacinamide 0.5%, tocopherol 0.1%, bisabolol 0.2%, card ripple 0.18%th, triethanolamine 0.14%, acetyl chitosamine 0.1%, parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%, EDTA- Disodium 0.05%, essence 0.02%, add deionized water to 100.

(5) the clear acne Cleansing Foam (part by weight) of recombined human hyaluronic acid compositions

Recombined human hyaluronic acid 0.01%, plant compound 4.0%, skin conditioning agent 1.0%, glycerine 25.0%, Pork and beans Cool sour 17.0%, palmitic acid 7.5%, stearic acid 7.3%, potassium hydroxide 7.2%, LC 3.0%, laurate 2.0%, oxybenzene first Ester 0.2%, essence 0.5%, polyethylene glycol -14M 0.1%, EDETATE SODIUM 0.1%, guar hydroxypropyltrimonium chloride 0.07%, add deionized water to 100.

(6) recombined human hyaluronic acid compositions make up remover (part by weight)

Recombined human hyaluronic acid 0.001%, plant compound 0.2%, skin conditioning agent 0.05%, D-pantothenyl aleohol 0.7%, chlorine Change sodium 0.55%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, Cosmocil CQ 0.7%, PLURONIC L64 0.04%th, 0.05%GREEN 6 0.38%, isomery hexadecane 10.0%, D5 16%, add deionized water to 100.

Embodiment 2

Oral care class formula

(1) recombined human hyaluronic acid compositions oropharynx maintenance spray (part by weight)

Recombined human hyaluronic acid 2.5%, plant compound 16.0%, skin conditioning agent 2.0%, glycerine 2.0%, sorb (sugar) alcohol 2.0%, honey powder 0.5%, peppermint 0.1%, potassium sorbate 0.5%, sodium benzoate 0.2%, add deionized water extremely 100。

(2) recombined human hyaluronic acid compositions care toothpaste (part by weight)

Recombined human hyaluronic acid 1.5%, plant compound 8.0%, skin conditioning agent 1.5%, ascorbic acid 1.0%, the moon Cinnamic alcohol sodium sulfovinate 2.0%, hydrated silica 18%, sorb (sugar) alcohol 30%, xylitol 5.0%, polyethylene glycol 400 5.0%, Chitosan 1.0%, hydroxyethyl cellulose 0.5%, sodium alginate 3.0, peppermint 1.5%, saccharin sodium 0.2%, add deionized water extremely 100。

(3) recombined human hyaluronic acid compositions are brushed teeth tooth piece (part by weight)

Recombined human hyaluronic acid 9.0%, plant compound 2.0%, skin conditioning agent 1.0%, microcrystalline cellulose 25.5%, mountain Pears sugar alcohol 24.5%, xylitol 24.0%, stevioside 0.25%, sodium lauroyl glutamate 1.0%, peppermint 5.0%, sodium alginate 3.0%th, sodium acid carbonate 1.0%, ascorbic acid 1.0%, radix glycyrrhizae 0.5%, magnesium stearate 1.0%, silica 0.5, essence 0.75%.

(4) recombined human hyaluronic acid compositions nursing condensation (part by weight)

Recombined human hyaluronic acid 3.0%, plant compound 1.0%, skin conditioning agent 2.0%, glycerine 6.0%, card ripple 1.0%th, sorb (sugar) alcohol 2.0%, tea extract 0.1%, acesulfame potassium 0.03%, potassium sorbate 0.03%, sodium benzoate 0.02%th, essence 0.08%, propane diols 2.0%, Tween-80 0.2%, triethanolamine 0.9%, sodium lactate 0.2%, add from Sub- water is to 100.

Embodiment 3

Hair washes shield class formula

(1) recombined human hyaluronic acid compositions scalp nursing solution (part by weight)

Recombined human hyaluronic acid 2.0%, plant compound 3.0%, skin conditioning agent 5.0%, glycerine 1.5%, F-68 0.05%th, Finasteride 0.01%, K220 0.1%, add deionized water to 100.

(2) recombined human hyaluronic acid compositions shampoo (part by weight)

Recombined human hyaluronic acid 0.5%, plant compound 8.0%, skin conditioning agent 2.0%, AES (70%) 18%, CAB (30%) 5.0%, 6,501 2.0%, pearly-lustre dibasic acid esters 1.5%, silicone emulsion (60%) 2.0%, guar gum C-14-S 0.3%, Citric acid 0.2%, sodium chloride 0.8%, shredding (1.5%) 0.08%, essence 0.5%, EDTA- disodiums 0.1%, add deionized water To 100.

(3) recombined human hyaluronic acid compositions hair conditioner (part by weight)

Recombined human hyaluronic acid 0.6%, plant compound 2.0%, skin conditioning agent 5.0%, aloe extract 20.0%, Octadecyl alcolol 5.0%, cationic surfactant 8.0%, lanolin 2.0%, sodium benzoate 0.2%, phenmethylol 0.3%, chlorination Sodium 1.0%, essence 0.02%, pigment 0.01%, add deionized water to 100.

(4) recombined human hyaluronic acid compositions pomade (part by weight)

Recombined human hyaluronic acid 0.1%, plant compound 1.0%, skin conditioning agent 1.5%, peregal 25.0%, A25 1.0%th, EP-200 2.6%, cera alba 8.0%, methyl esters 0.2%, propyl ester 0.1%, lanolin 1.0%, propane diols 10.0%, VA64 2.0%, essence 0.02%, add deionized water to 100.

Embodiment 4

Body care class formula

(1) the refreshing body spraying (part by weight) of recombined human hyaluronic acid compositions

Recombined human hyaluronic acid 0.5%, plant compound 20.0%, skin conditioning agent 6.0%, 1,2- pentanediols 2.0%, Avenabeta glucosan 2.0%, butanediol 2.0%, trehalose 1.5%, parahydroxyacet-ophenone 0.3%, 1,2- hexylene glycols 0.3%, Dipotassium glycyrrhizinate 0.1%, allantoin 0.1%, Cremophor RH40 0.09%, panthenol 0.05%, essence 0.01%, three second Hydramine 0.005%, add deionized water to 100.

(2) recombined human hyaluronic acid compositions shower cream (part by weight)

Recombined human hyaluronic acid 0.5%, plant compound 8.0%, skin conditioning agent 6.0%, methyl cocoyl ox sulphur Sour sodium 6.0%, LAA 6.0%, sodium lauroyl sarcosine 5.0%, coconut oleoyl amine methyl MEA3.0%, the last of the ten Heavenly stems Base glyceryl monooleate 2.0%, Natrulon H-10 oleate 2.0%, caprylic/capric glyceride type Natrulon H-10 esters 2.0%, PEG-120 methyls dioleate 1.5%, cocounut oil acyl isethionic acid ester sodium 1.0%, lauryl glucoside 1.0%, MS6211.0%, Polyquaternium-10 0.4%, sodium citrate 0.1%, EDETATE SODIUM 0.1%, glycerol monolaurate 0.3%, lemon Lemon acid 0.3%, panthenol 0.5%, dehydroactic acid 0.5%, phenmethylol 0.3%, essence 0.25%, add deionized water to 100.

(3) recombined human hyaluronic acid compositions body lotion (part by weight)

Recombined human hyaluronic acid 1.5%, plant compound 8.0%, skin conditioning agent 8.0%, glycerine 5.0%, the octanoic acid/last of the ten Heavenly stems Acid glyceride 5.0%, shea butter 3.0%, butanediol 3.0%, white oil 2.0%, cetostearyl alcohol 1.0%, silicone emulsion 1.0%th, AVC0.6%, isononyl isononanoate 1.0%, stearine 1.0%, polysorbate -60 2.0%, stearyl alcohol Polyethers -210.5%, tocopherol 0.5%, panthenol 0.5%, olive oil 0.5%, cyclopentasiloxane 0.2%, lecithin 0.2%, benzene Oxyethanol 0.3%, Sensiva SC50 0.2%, EDTA- disodiums 0.1%, add deionized water to 100.

(4) recombined human hyaluronic acid compositions suncream (part by weight)

Recombined human hyaluronic acid 0.2%, plant compound 1.0%, skin conditioning agent 2.0%, stearic acid 10.0%, 16 Alcohol 1.5%, lanolin 1.0%, white oil 5.0%, glycerin monostearate 0.5%, Tween-80 2.7%, private disk -60 0.5%th, K12 0.3%, glycerine 2.5%, phenyl salicylate 1.0%, titanium dioxide 1.0%, add deionized water to 100.

(5) soap (part by weight) is used in recombined human hyaluronic acid compositions liquid shower

Recombined human hyaluronic acid 1.5%, plant compound 6.0%, skin conditioning agent 6.0%, K12 (30%) 42.0%, COCOAMIDOPROPYL BETAINE 8.0%, DOW1.2%, sodium chloride 1.0%, dehydroactic acid 0.5%, phenmethylol 0.3%, essence 0.2%th, pigment 0.02%, deionized water is added to 100.

(6) recombined human hyaluronic acid compositions protect sufficient spray (part by weight)

Recombined human hyaluronic acid 2.5%, plant compound 20.0%, skin conditioning agent 10.0%, glycerine 1.5%, F-68 0.05%th, ketoconazole 0.25%, K220 0.1%, add deionized water to 100.

Embodiment 5

Shield class formula is washed in private portion

(1) recombined human hyaluronic acid compositions private portion conditioning liquid (part by weight)

Recombined human hyaluronic acid 2.0%, plant compound 5.0%, skin conditioning agent 3.0%, glycerine 1.5%, boric acid 1.0%th, Phenoxyethanol 0.3%, methyl hydroxybenzoate 0.3%, ethyl hydroxy benzoate 0.2%, Sensiva SC50 0.2%, add deionized water To 100.

(2) recombined human hyaluronic acid compositions private portion foam (part by weight)

Recombined human hyaluronic acid 1.5%, plant compound 15.0%, skin conditioning agent 5.0%, sodium lauroyl sarcosine 6.0%th, SP-115B3.0%, LAA 2.0%, glycerine 1.5%, 1,3- propane diols 1.0%, Phenoxyethanol 0.3%th, methyl hydroxybenzoate 0.3%, ethyl hydroxy benzoate 0.2%, Sensiva SC50 0.2%, parachlorometaxylenol 0.3%, lactic acid 0.05%th, sodium lactate 0.05%, deionized water is added to 100.

(3) recombined human hyaluronic acid compositions private portion gel (part by weight)

Recombined human hyaluronic acid 1.5%, plant compound 3.0%, skin conditioning agent 6.0%, borax 2.5%, carbomer 1.7%th, glycerine 1.5%, Phenoxyethanol 0.3%, methyl hydroxybenzoate 0.3%, ethyl hydroxy benzoate 0.2%, Sensiva SC50 0.2%, Add deionized water to 100.

(4) recombined human hyaluronic acid compositions sterilization conception control gel (part by weight)

Recombined human hyaluronic acid 2.0%, plant compound 10.0%, skin conditioning agent 3.0%, nonoxinol 9 0.1%, Carbomer 1.5%, glycerine 3.0%, D-sorbite 2.0%, sodium chloride 0.2%, potassium hydroxide 0.1%, add deionized water extremely 100。

Embodiment 6

Recombined human hyaluronic acid compositions preparation method

A, purity 90.0%-99.9% 200-600KD macromolecular hyaluronic acid is taken to use recombined human hyaluronidase PH20 is cut, and uses recombined human hyaluronidase 1000-2000 units per g hyaluronic acids, the 1.0% of 50g is dissolved in 35-40 DEG C Sodium chloride solution in, 1000-3000r/min stirring digestion 3-4h, adjust pH5.0-5.6, add deionized water equal to 100g mixing It is even, recombined human hyaluronic acid solution of the concentration range in 4.5%-9.0% is prepared into, is saved backup；

B, aloe silk cunning element, dragon tree, strawberry fruit, resurrection grass, frankincense, motherwort, purslane, horse chestnut are in mass ratio 2-3:0.5-1:1-2:1-2:0.5-1:0.5-1:1-3:2-3 forms plant compound, wherein each composition optimum weight ratio 2.5: 0.5:1:1:0.5:0.5:1.5:2.5, co-grinding adds 10 times of amount water, boiling 2 hours, filtration treatment, filter into 1000 grams of coarse powder Slag adds 8 times of amount water, boiling 1 hour, filtration treatment；Filter residue add again 8 times amount water, boiling 1 hour, filtration treatment, filtrate merge, The clear cream that relative density is 1.0g/ml is concentrated under reduced pressure at 50 DEG C, in use, plus ethanol make alcohol content up to 60%, stand 24 Hour, filtering, decompression filtrate recycling ethanol is simultaneously concentrated into no alcohol taste, standby；

C, collagen, polyglutamic acid, allantoin, malt polysaccharide, natural mineral water 1-3 in mass ratio:0.5-1:1- 2:5-10:10-20 forms skin conditioning agent, optimum weight ratio 1:0.5:1:5:10 weigh collagen, polyglutamic acid, urine respectively Bursin, malt polysaccharide and natural mineral water, first with 30-50 DEG C of dissolving polyglutamic acid of 10-20 times of natural mineral water, add allantoin It is well mixed, collagen and malt polysaccharide are added after cooling, is well mixed transparent solution, as skin conditioning agent, it is standby；

D, above-mentioned recombined human hyaluronic acid solution and skin conditioning agent are taken, temperature control adds plant compounding at 30-50 DEG C Thing is ultrasonically treated 20-50min under 60-80HZ so that solution is well mixed；

Above-mentioned solution is slowly added dropwise in the formulation configured in advance with matrix auxiliary material by e, and being gently mixed makes its complete It is complete scattered；

F, pH4.0-9.0 is adjusted, it is standby that corresponding dosage form product is made.

Test example 1

First, moisture retention is tested

(man 5, female 15 by the men and women 20 without disease of skin that experimental selection can be influenceed by result of the test completely Name) tested for object, imitated using the moisturizing for testing recombined human hyaluronic acid compositions solution (matrix auxiliary material is deionized water) Fruit.Specific method is as follows：Each experimental subjects first with behind water cleaning experiment position, then adapts to external environment condition 30 and divided before sample Clock.The environmental condition of experiment is：22-24 DEG C of indoor temperature, indoor humidity 45-50% are kept, and is tested in same place. In inboard arm with 2*2cm²Sample each 10ul as shown in table 1 is smeared, and is dried 1 minute.Then respectively applied again at the position of smearing Spread 20ul ultra-pure water, with after towel moisture with 1 point for interval (0 minute, 1 minute, 2 minutes, 3 minutes, 4 minutes, 5 Minute, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes), the skin with Corneometer CM 825 to each smearing position Surface moisture is measured moistening effect.Measurement result is as shown in table 2, and unit is Corneometer value (Cv).

The each sample composition solution composition of table 1 composition situation is as follows：

Note："-" represents to be not added with

The each sample composition solution moistening effect measurement result of table 2 is as follows：

	0 point	2	4	6	8	10
							Sample 1	156.6	66.5	58.8	50.2	48.7	46.3
Sample 2	160.8	70.5	60.8	52.5	49.3	47.8
							Sample 3	157.8	68.2	59.8	51.5	49.6	46.9
Sample 4	163.8	74.5	63.2	54.1	50.2	48.9
							Sample 5	163.9	74.9	63.5	54.6	50.5	49.5
Sample 6	165.4	76.8	65.9	56.4	51.4	50.8
							Sample 7	164.4	75.4	64.6	55.6	50.9	50.1
Sample 8	167.6	79.8	67.5	57.2	52.2	51.5
							Sample 9	166.6	78.8	67.1	56.9	51.6	50.9
Sample 10	168.0	80.2	67.9	57.8	53.7	52.6
							Sample 11	168.5	80.9	68.4	58.3	55.9	55.1
Sample 12	172.6	72.5	62.8	56.2	52.7	51.3
							Sample 13	176.9	76.9	66.5	62.4	58.2	56.8
Sample 14	181.6	82.4	72.0	65.8	61.3	60.1
							Sample 15	180.0	81.2	70.0	64.4	60.1	59.7
Sample 16	186.2	85.8	73.5	67.2	63.5	62.9
							Sample 17	187.8	87.5	75.8	69.5	64.1	63.8
Sample 18	190.5	92.6	78.3	72.5	68.2	67.5
							Control group 1	156.6	66.5	58.8	50.2	48.7	46.3
Control group 2	144.4	60.6	51.5	43.9	42.1	40.5
							Control group 3	160.9	72.9	62.5	53.4	51.2	49.5

Conclusion：

(1) certain weight ratio is pressed from table 1,2, recombined human hyaluronic acid, plant compound and skin conditioning agent three The composition solution of composition is more preferable than single recombined human hyaluronic acid, plant compound, skin condition agent solution moistening effect, Illustrate that recombined human hyaluronic acid, plant compound, skin conditioning agent three have significantly in terms of being combined in moistening effect Chemiluminescence；

(2) from table 1,2, recombined human hyaluronic acid of the mass fraction in the range of 0.001-9.0%, in 0.2- The composition solution of plant compound in the range of 20.0%, the composition of the skin conditioning agent three in the range of 0.05-10.0% Moistening effect is respectively provided with chemiluminescence；

(3) comparative sample understands that recombined human hyaluronic acid, plant compound, skin conditioning agent three's addition are 1.0%th, 10.0%, 5.0% when, moisturizing chemiluminescence is most reasonable, inadequate less than this value moistening effect, higher than this value Moistening effect amplification slows down and the problems such as expecting body waste, cost increase occurs.

2nd, percutaneous absorbability is tested

The mouse skin cuticula handled well is fixed on Franz diffusion cells upwards according to Transdermal Absorption experiment, connect 30% ethanol-physiological saline is added in receives pond, is placed in the intelligent penetrating absorption instrument of water bath with thermostatic control, design temperature (37.0 ± 0.5) DEG C, rotating speed 200rmin^{- 1}.1h is balanced, sample is added after draining the bubble in reception tank.On diffusion cell supply area Even smearing sample each 1g as shown in table 3, every group parallel 3 times, respectively 0,0.1,0.3,0.5,1.0,2.0,4.0,6.0,8.0, 10.0th, 12.0 when draw reception liquid 1mL, and fill into the reception liquid of equality of temperature same volume.The reception liquid of absorption adds examination after filtering Pipe, then it is accurate add 2mLCTAB test solutions, enough long-times are rocked in the timing from when adding, gently vibration, and during which trying one's best avoids Reaction solution produces foam, stands to close to 10min, then pours into reaction solution in 1cm cuvettes, and measure wavelength exists during 10min Absorbance (A during 400nm₄₀₀), the Transdermal absorption of hyaluronic acid is obtained according to the standard curve of hyaluronic acid at that wavelength Rate.

The each sample composition solution composition of table 3 composition situation is as follows:

	Recombined human hyaluronic acid	Plant compound	Skin conditioning agent	Deionized water
					Sample 1	1.0%	10.0%	5.0%	To 100
Sample 2	1.0%	10.0%	-	To 100
					Sample 3	1.0%	-	5.0%	To 100
Control group	1.0%	-	-	To 100

Note："-" represents to be not added with

Conclusion：Using hyaluronic acid transdermal absorption factor as ordinate, the time (t) is abscissa, draws the transdermal suction of hyaluronic acid Yield-time graph, see percutaneous absorbability experimental result (Fig. 1), the absorptivity for as a result showing hyaluronic acid in 12h is sample one The ＞ control groups of three ＞ samples of ＞ samples two, show recombined human hyaluronic acid, plant compound, the group of skin conditioning agent three composition Compound is remarkably improved the transdermal absorption factor of hyaluronic acid；As a result show that absorption of the 0.5h inner skins to hyaluronic acid is most fast, should Recombined human hyaluronic acid compositions influence most notable in 0.5h on the transdermal absorption factor of hyaluronic acid；Experiment is by hyalomitome The research of sour transdermal absorption factor shows that there is the recombined human hyaluronic acid compositions certain rush imbibition to receive effect.

3rd, the compound prescription experiment of recombined human hyaluronic acid compositions solution is studied

Bioactivity of the recombined human hyaluronic acid compositions solution in human body, i.e. town are now detected using the method for human experimentation Surely releive, anti-inflammatory reparation, nourish opsonic activity, study the activity conditioning spraying with prevent and conditioning skin discomfort.

All experimenters of this experiment are different degrees of skin discomfort person, each sample recombined human hyaluronic acid compositions into It is grouped into as shown in table 4, the use recombined human hyaluronic acid 1.0%+ plant compound 10.0%+ skin conditioning agents of experimental group 1 The sample solution of 5.0% combination is smeared 1 time before sleeping every night；Experimental group 2 uses recombined human hyaluronic acid 1.0%+ plant compounds The sample solution of 10.0% combination is smeared 1 time before sleeping every night；Experimental group 3 uses recombined human hyaluronic acid 1.0%+ skin conditioning agents The sample solution of 5.0% combination is smeared 1 time before sleeping every night；Positive control uses the sample that recombined human hyaluronic acid 1.0% combines Solution is smeared 1 time before sleeping every night；Negative control is smeared 1 time before being slept every night using physiological saline；Compare symptom after five groups of conditionings 7 days Improvement situation.

Each experimental group sample solution composition composition situation of table 4 is as follows:

	Recombined human hyaluronic acid	Plant compound	Skin conditioning agent	Deionized water
					Experimental group 1	1.0%	10.0%	5.0%	To 100
Experimental group 2	1.0%	10.0%	-	To 100
					Experimental group 3	1.0%	-	5.0%	To 100
Positive controls	1.0%	-	-	To 100
					Negative control group	-	-	-	Physiological saline is to 100

Note："-" represents to be not added with

The symptom assessments such as dermal sensation discomfort, allergy, inflammation, peculiar smell, spot：

It is effective：Symptom is significantly improved or disappeared, experimenter's satisfaction；Effectively：Symptom has improvement or mitigated, and experimenter still has not It is suitable, it is desirable to continue to nurse one's health；It is invalid：Symptom mitigates without obvious, and experimenter is unsatisfied with.

Statistical method：Statistical procedures are carried out using SPSS12.0 statistics softwares, compared between metering and enumeration data group Examined compared with being examined using t with x2, with p<0.05 is statistically significant difference.

As a result it is as follows：

As shown in table 5, the 8th day symptom improves situation：Experimental group 1 effective 10, total effective rate 100%；Experimental group 2 is effective 7, effective 2, invalid 1, total effective rate 90%, obvious effective rate P compared with experimental group 1<0.01；Experimental group 3 effective 8, has Effect 1, invalid 1, total effective rate 90%, obvious effective rate P compared with experimental group 1<0.01；Effective 2 of positive control, effective 4, Invalid 4, total effective rate 60%, obvious effective rate P compared with experimental group 1<0.01.

The 8th day each group experimenter symptom of table 5 improves situation and is compared as follows：

Conclusion：

(the sample that recombined human hyaluronic acid 1.0%+ plant compound 10.0%+ skin conditioning agents 5.0% combine of experimental group 1 Product solution) substantially than experimental group 2 (sample solution that recombined human hyaluronic acid 1.0%+ plants compound 10.0% combines), experiment Group 3 (sample solution that recombined human hyaluronic acid 1.0%+ skin conditioning agents 5.0% combine), positive control are effective.The present invention's Recombined human hyaluronic acid compositions solution human experimentation shows (1) recombined human hyaluronic acid, plant compound and skin condition The solution of agent three combination is to prevention and conditioning skin discomfort significant effect；(2) recombined human hyaluronic acid, plant compound and skin The solution of skin conditioner three combination is calmed in a short time releives, anti-inflammatory reparation, nourishes conditioning skin problem and have certain effect, long Phase using effect can be more preferably；(3) solution of recombined human hyaluronic acid, plant compound and skin conditioning agent three combination is preventing There is obvious chemiluminescence with the uncomfortable aspect of conditioning skin.

4th, the optimum proportioning experiment of the solution combined formula of recombined human hyaluronic acid compositions is studied

0.1%, 1.0%, 3.0% recombined human hyaluronic acid will be matched respectively, proportioning 2.0%, 10.0%, 18.0% Plant compound, the skin conditioning agent of proportioning 0.5%, 5.0%, 10.0% are configured to recombined human hyaluronic acid compositions, other Matrix auxiliary material is deionized water, all the components mass percent summation 100%.All experimenters of this experiment are different degrees of Skin discomfort person, the different ratio that experiment 1-27 is combined with recombined human hyaluronic acid, plant compound and skin conditioning agent three Composition solution daily sooner or later smear 2 times progress Three factors-levels orthogonal experiments, observation conditioning after situation；It is positive right The solution that the recombined human hyaluronic acid of proportioning 0.1%, 1.0%, 3.0% is respectively adopted according to 28-30 is smeared 2 times sooner or later daily, is seen Examine the situation after conditioning；31 be that negative control is smeared 2 times sooner or later daily using physiological saline, the situation after observation conditioning；Every group 5 Individual patient, comparing symptom after nursing one's health 7 days improves situation.

The orthogonal experiment factor meter of table 6：

The symptom assessment standards such as dermal sensation discomfort, allergy, inflammation, peculiar smell, spot are same as above.

The 8th day each group experimenter symptom of table 7 improves situation and is compared as follows：

Conclusion：

Experiment is used through human body, the results showed that uses the recombined human hyaluronic acid of proportioning 0.1%, 1.0%, 3.0%, proportioning 2.0%th, 10.0%, 18.0% plant compound, the weight that 0.5%, 5.0%, 10.0% skin conditioning agent is configured to is matched Group people's hyaluronic acid compositions solution it is calm releive, anti-inflammatory reparation, nourish conditioning skin problem in terms of have the effect of certain, But this scope is not limited to；When proportioning is 1.0% recombined human hyaluronic acid, the plant compound for 10.0% is matched, matches and is During the recombined human hyaluronic acid compositions solution that 5.0% skin conditioning agent is configured to, effect embodiment is most obvious, and the proportioning is most Good proportioning.

5th, bacteriostatic experiment

Take Clinical isolation Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans a little, connect respectively Kind cultivates 18h in broth bouillon at 37 DEG C.(calf serum that 20% is added in Pseudomonas aeruginosa broth bouillon) takes Each bacterial strain nutrient broth culture of 18h cultures, it is made into bacteria suspension and is used to test.Take sterilizing test tubes 11 respectively, the 1st Nutrient broth fluid nutrient medium 9ml is added, 2-10 branch adds 5ml, the 11st addition 10ml, separately sampled product solution such as table 8 Shown 1ml adds the 1st test tube, takes 5ml to add the 2nd after well mixed, is diluted to the 10th successively, the 11st is not added with sample As control.Often pipe adds escherichia coli suspension 0.1ml, and 24h is cultivated at a temperature of 37 DEG C, takes out observation bacterial growth situation. Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans are the same as above-mentioned Germicidal efficacy growing state.As test tube becomes cloudy, that is, represent Bacterial growth, sample is without bacteriostasis；As test tube is limpid, expression bacterial growth is suppressed.Experimental result is as shown in table 9, this Invention recombined human hyaluronic acid compositions solution is to Clinical isolation Escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, white Color candida albicans has stronger bacteriostasis.

The bacteriostatic experiment each sample solution composition of table 8 forms

	Recombined human hyaluronic acid	Plant compound	Skin conditioning agent	Deionized water
					Sample 1	1.0%	10.0%	5.0%	To 100
Sample 2	1.0%	10.0%	-	To 100
					Sample 3	1.0%	-	5.0%	To 100
Sample 4	-	10.0%	5.0%	To 100
					Sample 5	1.0%	-	-	To 100
Sample 6	-	10.0%	-	To 100
					Sample 7	-	-	5.0%	To 100

Note："-" represents to be not added with

The each sample solution of table 9 corresponds to last limpid test tube numbering in bacteriostatic test

	Escherichia coli	Staphylococcus aureus	Pseudomonas aeruginosa	Candida albicans
					Sample 1	7	6	6	7
Sample 2	5	4	4	5
					Sample 3	2	2	1	2
Sample 4	4	3	3	3
					Sample 5	2	1	1	1
Sample 6	3	2	1	2
					Sample 7	-	-	-	-

Note："-" represents not limpid

By above-mentioned limpid broth tubes and control group the transferred species broth agar plates after diluting according to a certain percentage, observation 24h, the Cmin of no bacterial growth is bacteriocidal concentration, is designated as C, unit mg/mL, as a result as shown in table 10, illustrates this Invention recombined human hyaluronic acid compositions solution is to being clinically separated common Bacteria skin infection strain Escherichia coli, Staphylococcus aureus Bacterium, Pseudomonas aeruginosa, Candida albicans have stronger bactericidal action, can effectively suppress the activity of these Bacteria skin infections with pre- Anti- and conditioning skin is uncomfortable；Simultaneously by contrast as can be seen that recombined human hyaluronic acid, plant compound and skin conditioning agent three The recombined human hyaluronic acid compositions solution of person's composition also has chemiluminescence at antibacterial aspect.

The each sample solution sterilization conditions of table 10

	C (Escherichia coli)	C (staphylococcus aureus)	C (Pseudomonas aeruginosa)	C (Candida albicans)
					Sample 1	0.12	0.15	0.17	0.11
Sample 2	0.22	0.37	0.40	0.25
					Sample 3	0.76	0.50	0.80	0.66
Sample 4	0.47	0.52	0.55	0.60
					Sample 5	0.78	0.82	0.88	0.91
Sample 6	0.46	0.52	0.77	0.51
					Sample 7	-	-	-	-
Control group	-	-	-	-

Note："-" indicates no bactericidal properties

6th, acute oral toxicity is tested

According to test basis《Disinfection technology standard》(defend method prison hair in the 3.4 of (third edition) first fascicle experimental technique specification [1999] No. 448).By animal (regular grade NIH mouse 20, male and female half and half, 18~22g of body weight) water supply fasting 12 hours, Distilled water is solvent, and (recombined human hyaluronic acid 1.0%, plant compound 5.0%, skin conditioning agent 10.0% combine tested material The composition solution being configured to) dosage is set as 5500mg/kg, tested material is once filled with gavage syringe needle by 0.2ml/20g body weight To animal.Observed immediately after administration and record the poisoning manifestations, death toll and death time of animal, observe secondary, observation daily Two weeks phases.Toxicity grading is carried out according to acute toxicity grading criteria.Experimental result：In observation period, test mice does not occur substantially Symptom, no dead generation.

The tested material of table 11 is to NIH chmice acute Oral toxicity result of the tests

Sex	Dosage (mg/kg)	Number of animals (only)	Death toll (only)	The death rate (%)
					It is female	5500	10	0	0
It is male	5500	10	0	0

Conclusion：The solution of recombined human hyaluronic acid compositions of the present invention is to the acute through LD50 ＞ 5000mg/ of NIH mouse Kg, the true nontoxic level in border.

7th, vaginal mucosa stimulation test

Press《Disinfection technology standard》(third edition) first fascicle《Experimental technique specification》The 3.5th in (Ministry of Public Health, 2012.4) Carry out.Healthy White Rabbit 28 is chosen, is divided into experimental group 24, excipient group 2 and blank control group 2.Experimental group：It will apply The roundlet sliver for having the 2.0cm length of sample 1-12 test specimens inserts experimental animal intravaginal, every kind of each 2 of sample；Excipient Group：Soaked with an equal amount of sliver with sterile saline, insert animal intravaginal；Blank group：Do not process.Removed after 4 hours Sliver is removed, animal is put to death after 24 hours, takes out topical vaginal tissue, phenomena such as whetheing there is hyperemia, oedema observed.

The vaginal mucosa stimulation test each sample composition of table 12 forms

	Recombined human hyaluronic acid	Plant compound	Skin conditioning agent	Deionized water
					Sample 1	1.0%	2.0	5.0%	To 100
Sample 2	1.0%	10.0	5.0%	To 100
					Sample 3	1.0%	20.0	5.0%	To 100
Sample 4	1.0%	2.0	-	To 100
					Sample 5	1.0%	10.0	-	To 100
Sample 6	1.0%	20.0	-	To 100
					Sample 7	-	2.0	5.0%	To 100
Sample 8	-	10.0	5.0%	To 100
					Sample 9	-	20.0	5.0%	To 100
Sample 10	-	2.0	-	To 100
					Sample 11	-	10.0	-	To 100
Sample 12	-	20.0	-	To 100

The vaginal mucosa stimulation test result of table 13 is as follows：

Note："-" indicates no erythema or edematous condition；" 0 " represents no vaginal irritation reaction

In observation period, test group sample 1-5, sample 7-8, sample 10-11, excipient group, blank control group animal are local Vagina tissue does not occur erythema or edematous condition, and stimulate the reaction integral mean value is 0, according to《Disinfection technology standard》(third edition) One fascicle《Experimental technique specification》Relevant criterion judges in (Ministry of Public Health, 2012.4), and these samples stick to healthy White Rabbit vagina Film is nonirritant；And the appearance erythema or oedema that test group sample 6, sample 9, the animal topical vaginal tissue of sample 12 are different degrees of Symptom, stimulate the reaction integration are not 0, according to《Disinfection technology standard》(third edition) first fascicle《Experimental technique specification》(health Portion, 2012.4) relevant criterion judges in, and these samples have different degrees of excitant to healthy White Rabbit vaginal mucosa.

As a result show, the recombined human hyaluronic acid compositions solution of high content plant compound can make vagina tissue appearance red Spot or edematous condition, excitant can be produced to vaginal mucosa, excitant is obvious when plant compound content reaches 20.0%；Also It can be seen that the plant compound that the recombined human hyaluronic acid and skin conditioning agent of certain content can effectively alleviate high concentration draws The vagina tissue erythema that rises, the side effect such as oedema, avoid irritating generation；Recombined human hyaluronic acid compositions of the present invention simultaneously In each composition can also cooperate with reparation nourish vagina be damaged mucous membrane, strengthen vaginal mucosa immunity, containment RTI and Inflammatory reaction, maintain body normal health.

Embodiments of the invention are the foregoing is only, not thereby limit the scope of patent protection of the present invention, every utilization The equivalent structure or equivalent flow conversion that description of the invention and accompanying drawing content are made, or directly or indirectly it is used in other correlations Technical field, be included within the scope of the present invention.

Claims

A kind of 1. recombined human hyaluronic acid compositions, it is characterised in that said composition include following weight than component：

Described recombined human hyaluronic acid is cut big by the recombined human hyaluronidase PH20 for having saccharification produced using Chinese hamster ovary celI Molecular weight hyaluronic acid obtains, and the recombined human hyaluronic acid at least one end can identify hyaluronic acid normal configuration by use The end of recombined human hyaluronic acid cleavage.
2. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described recombined human hyaluronic acid Molecular weight is 0.7KD-100KD.
3. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described plant compound is from activity Gained is extracted in plant, described active plant comprises at least aloe silk and slides element, dragon tree, strawberry fruit, resurrection grass, frankincense, benefit Brittle Falsepimpernel Herb, purslane, horse chestnut.
4. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described skin conditioning agent at least wraps Include collagen, polyglutamic acid, allantoin, malt polysaccharide, natural mineral water.
5. skin conditioning agent as claimed in claim 4, it is characterised in that described natural mineral water is selected from Alps ice Chuan Shui, Changbai Mountain natural mineral water, Russian Caucasus Mountain mineral water, A Lewaer thermal waters, Canadian ooze mineral water One or both of more than.
6. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described matrix auxiliary material is in cosmetics In application be facial-care class matrix auxiliary material, the facial-care class matrix auxiliary material is selected from deionized water, glycerine, butanediol, sweet tea Dish alkali, glycerin polyether -26, dipotassium glycyrrhizinate, avenabeta glucosan, parahydroxyacet-ophenone, 1,2- hexylene glycols, PEG-40 hydrogenation castors Sesame oil, potassium hydroxide, EDTA- disodiums, pentanediol, PEG-4000, D-sorbite, carbomer, hydroxyethyl cellulose, sulphur are pungent Acid, hydrogenated olive oil, triethanolamine, essence, isononyl isononanoate, saualane, dimethyl silicone polymer, MONTANOV L, SIMULGEL EG, niacinamide, tocopherol, bisabolol, acetyl chitosamine, myristic acid, palmitic acid, stearic acid, hydroxide Potassium, LC, laurate, methyl hydroxybenzoate polyethylene glycol -14M, guar hydroxypropyltrimonium chloride, D-pantothenyl aleohol, sodium chloride, phosphoric acid Potassium dihydrogen, dipotassium hydrogen phosphate, Cosmocil CQ, PLURONIC L64,0.05%GREEN 6, isomery hexadecane, ring five poly- two It is more than one or both of methylsiloxane.
7. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described matrix auxiliary material is in cosmetics In application be oral care class matrix auxiliary material, the oral care class matrix auxiliary material is selected from deionized water, glycerine, sorb (sugar) Alcohol, honey powder, peppermint, potassium sorbate, sodium benzoate, ascorbic acid, laruyl alcohol sodium sulfovinate, hydrated silica, xylitol, poly- second Glycol 400, chitosan, hydroxyethyl cellulose, sodium alginate, peppermint, saccharin sodium, microcrystalline cellulose, stevioside, lauroyl glutamate Sodium, sodium acid carbonate, ascorbic acid, radix glycyrrhizae, magnesium stearate, silica, card ripple, tea extract, acesulfame potassium, propane diols, tell It is more than one or both of temperature -80, triethanolamine, sodium lactate.
8. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described matrix auxiliary material is in cosmetics In application wash shield class matrix auxiliary material for hair, the hair wash shield class matrix auxiliary material be selected from deionized water, glycerine, F-68, it is non-that Male amine, K220, AES, CAB, 6501, pearly-lustre dibasic acid esters, silicone emulsion, guar gum C-14-S, citric acid, sodium chloride, shredding, perfume (or spice) Essence, EDTA- disodiums, aloe extract, octadecyl alcolol, cationic surfactant, lanolin, sodium benzoate, phenmethylol, pigment, It is more than one or both of peregal, A25, EP-200, cera alba, methyl esters, propyl ester, lanolin, propane diols, VA64.
9. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described matrix auxiliary material is in cosmetics In application be body care class matrix auxiliary material, the body care class matrix auxiliary material is selected from deionized water, 1,2- pentanediols, oat Beta glucan, butanediol, trehalose, parahydroxyacet-ophenone, 1,2- hexylene glycols, dipotassium glycyrrhizinate, allantoin, PEG-40 hydrogenation castors Sesame oil, panthenol, essence, triethanolamine, sodium methyl cocoyl taurate, LAA, Hamposyl L Sodium, coconut oleoyl amine methyl MEA, decyl glyceryl monooleate, Natrulon H-10 oleate, caprylic/capric glyceride type polyglycereol- 10 esters, PEG-120 methyls dioleate, cocounut oil acyl isethionic acid ester sodium, lauryl glucoside, MS621, polyquaternary amine Salt -10, sodium citrate, EDETATE SODIUM, glycerol monolaurate, citric acid, dehydroactic acid, phenmethylol, caprylic/capric glyceride, Shea butter, white oil, cetostearyl alcohol, silicone emulsion, AVC, isononyl isononanoate, stearine, polysorbate- 60th, stereth -21, tocopherol, olive oil, cyclopentasiloxane, lecithin, Phenoxyethanol, Sensiva SC50, tristearin Acid, hexadecanol, lanolin, Tween-80, private disk -60, K12, glycerine, phenyl salicylate, titanium dioxide, coconut monoethanolamide base propyl group It is more than one or both of glycine betaine, DOW, sodium chloride, pigment, F-68, ketoconazole, K220.
10. recombined human hyaluronic acid compositions as claimed in claim 1, it is characterised in that described matrix auxiliary material is in daily use chemicals Application in articles for use washes shield class matrix auxiliary material for private portion, the private portion wash shield class matrix auxiliary material be selected from deionized water, glycerine, boric acid, Phenoxyethanol, methyl hydroxybenzoate, ethyl hydroxy benzoate, Sensiva SC50, sodium lauroyl sarcosine, SP-115B, lauroyl both sexes base second Sour sodium, 1,3- propane diols, parachlorometaxylenol, lactic acid, sodium lactate, borax, carbomer, Nonoxynol-9, D-sorbite, chlorine Change more than one or both of sodium, potassium hydroxide.