KR20070002409A - Cosmetic composition containing as available ingredient the extracts of Equisetum arvense L. - Google Patents

Abstract

A cosmetic composition comprising the extract of Equisetum arvense L. as an effective ingredient is provided to promote growth of keratinocytes, remove reactive oxygen species, increase biosynthesis of procollagen type 1 by reducing biosynthesis of MMP-1(matrix metaloproteinase-1), and inhibit aging. The cosmetic composition for promoting growth of keratinocytes, removing reactive oxygen species, increasing biosynthesis of procollagen type 1 by reducing biosynthesis of MMP-1, or inhibiting aging comprises the extract of Equisetum arvense L. and at least one extract selected from Lonicera japonica Thunberg extract, Prunus armeniaca extract, Plantago asiatica extract and Coix lacryma-jobi extract, wherein the amount of the medicinal herb extract is 0.0001 to 25 wt.% based on the total weight of the composition; the medicinal herb extracts are prepared with water; and the composition further comprises 1,3-butyleneglycol.

Description

Cosmetic composition containing as sentence active ingredient {Cosmetic composition containing as available ingredient the extracts of Equisetum arvense L.}

The present invention relates to a composition further comprising one or more selected from the group consisting of phosphorus extract, algae extract, gamguk extract and Uuiin extract as a active ingredient, more specifically keratinocyte growth promoting effect, scavenging free radicals Effects, the biosynthetic effect of type 1 procollagen through reducing the biosynthesis of MMP-1, and a composition having an anti-aging effect.

Skin is the body's primary protective film that protects internal organs from changes in temperature and humidity, irritation from the external environment such as ultraviolet rays and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, ultraviolet rays, excessive physical and chemical stimuli, stress, and malnutrition received from the outside degrade skin normal function and promote skin aging such as loss of elasticity, keratinization, and wrinkle formation.

In particular, keratinocytes are continuously made in turn, and the outermost layer of old keratinocytes sometimes breaks out, so if the keratinization process that maintains a constant thickness of the epidermis is not smooth, abnormal keratinocytes are generated and accumulated on the skin surface. The skin becomes thicker, rougher, dull, and finer wrinkles occur, which eventually leads to aging of the skin. In addition, the decrease in biosynthesis of type 1 procollagen through the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of free radicals and skin substrates produced by ultraviolet rays is also associated with skin aging.

Therefore, in order to prevent this phenomenon and maintain a healthier and more elastic skin, by using cosmetics reinforced with conventional bioactive substances obtained from various animals, plants, microorganisms, etc., it maintains the unique function of the skin and activates skin cells. Efforts have been made to effectively inhibit aging. However, conventional cosmetic raw materials have many problems, such as most of them are ineffective or cause skin side effects.

On the other hand, collagen biosynthesis accelerators containing sentence extracts are known (Japanese Patent Laid-Open No. 2001-15566), and wrinkle improvement agents containing the same are known (Japanese Patent Laid-Open Publication No. 1998-250476), but they are phosphorus extracts in the sentence extract. No compositions containing additional extracts of almonds, extracts of Korean ginseng or extracts of euonymus were found.

Thus, the present inventors have tried to find a raw material having an anti-aging effect without causing skin side effects among natural products, and as a result, the extract contains at least one selected from the group consisting of an artificial extract, a pedestrian extract, a gamguchi extract, and a uiyi extract. The composition was found to have keratinocyte growth promoting effect, active oxygen scavenging effect, reducing the biosynthesis of MMP-1 to promote the biosynthesis of type 1 procollagen and to prevent the aging effect has been completed the present invention.

Accordingly, an object of the present invention is to further include keratinocyte growth promoting effect, active oxygen scavenging effect, MMP- by further comprising at least one selected from the group consisting of phosphorus extract, algae extract, gamguk extract and Euiin extract in the sentence extract extract as an active ingredient It is to provide a composition having a biosynthesis promoting effect and an anti-aging effect of type 1 procollagen by reducing the biosynthesis of 1.

The present invention relates to a composition further comprising one or more selected from the group consisting of phosphorus extract, algae extract, gamguk extract and Uuiin extract as a active ingredient, more specifically keratinocyte growth promoting effect, scavenging free radicals Effects, the biosynthetic effect of type 1 procollagen through reducing the biosynthesis of MMP-1, and a composition having an anti-aging effect.

Moonset (Equisetum arvense L.) is a perennial plant of the genus Cornifolia, also known as parietal or horsetail. In oriental medicine, there is a record of diuretic, hemostatic, anti-inflammatory action, there is a clearing action to reduce blood pressure and is effective in sea water accompanied by fever and diuretic. In addition, it has a bleeding effect and shows a hemostatic response to nosebleeds, hemorrhage, urine, keratosis, hemorrhoidal bleeding and uterine bleeding. The civilian is good for atherosclerosis and hypertension, and there are various cases that have been effective for many diseases. Apigenin, Luteolin, Camperol-3-O-Glucoside and Quecetin-3-O-Glucoside (Apigenin), Luteolin, Quercetin- 3 -O-Glucosides have been reported for antitumor studies such as liver function protection.

Indong ( Lonicera japonica Thunberg) is a semi-evergreen vine shrub plant of Indongaceae native to wild fields all over Korea. Its flowers are called 'gold silver' and 'leaf and stem' are widely used as medicinal herbs. It is a nontoxic herbal medicine. In oriental medicine, humans have been used for the treatment of arthritis and bronchitis because they are effective for onggi (inflammation of the inside and the outside), and recently, epidemic colds, various purulent infections, redness of the joints due to moist heat, swelling, hepatotoxicity protection and cells It is known that the drug is also effective in reducing immunity. In addition, it is known that a person who is deaf to the heart and lung in the oriental medicine lowers the heat, loosens the poison, works through the meridians, and shows diuretic and bactericidal action. Diarrhea caused by heat poisoning, hemorrhage, swelling, hepatitis, boil It is a medicine that is used for hypoxia and arthritis. The active ingredients contained in the phosphorus include flavonoids such as apigenin, ronierin, and luteolin, and have anti-inflammatory and anticancer effects by these flavonoids. It is known to have a back.

Prunus armeniaca is generally used to collect the mature fruit of the apricot tree belonging to the Rosaceae, to remove the flesh and seedlings, and to dry only the seeds, and to promote the urokinase activity, analgesic effect, anti-fog effect, antitumor activity It is known to have such pharmacological effects. In Chinese medicine, passers-by to the lungs treats seawater and asthma, which is applied to both wind-heated and abundant seawater. It is known to be effective in removing sweats and poisoning. The active ingredients contained in the passerin include fatty oil, Amygdalin, Emusin, etc., and especially, it contains a large amount of unsaturated fatty acids, so it has excellent skin soothing and softening effect and whitening effect. It is. It is also known to contain flavonoids such as Hyperside, Astragalin, Trifolin, and Quercitrin.

Plantago asiatica is a perennial plant of the dicotyledon plant Campanula asteraceae, also known as yellow kingdom. Gam-guk, which is admired in the liver and lungs, has been used as a medicine for immortality since ancient times, such as headaches and neck pains, and drinking for the next generation in folk medicine. In addition, anti-inflammatory, antipyretic analgesic is used for headache, dizziness, etc., and has the effect of clearing, excursions, swelling, detoxification in herbal medicine, has been used to treat carbuncles, intestines, purposes, strength, pemphigus, eczema and the like. The active ingredients contained in persimmon soup include essential oils, acecetin-7-rhamnoglucoside, adenine, choline, vitamin A, and B1. Outposts include essential oils, Luteolin glycosides, and Chrysanthemin.

Coix lacryma-jobi is a species of yulmu belonging to the family of rice family. It is known to be used. In addition, it is good for swelling and severe asthma of the body to overcome the humidity, anti-inflammatory, drainage, has a diuretic and antibacterial action to discharge foreign substances and waste in the body. In clinical practice, anticancer action, blood pressure lowering action, uterine contraction action, bowel movement control action have been reported. The active ingredients contained in yiyiin Coixol (Coixol), Cocoxerolide (Coxenolide), Sitosterol (Sitosterol), Stigmasterol (Stigmasterol) has been reported separately.

The extract according to the present invention is characterized in that the water is extracted as the extraction solvent, wherein the weight ratio of the herbal medicine and the extraction solvent water is characterized in that 1:10 to 1:20.

In addition, the composition containing the extract according to the invention is characterized in that it further contains 1,3-butylene glycol, wherein the 1,3-butylene glycol is 15 to 30% by weight of 100% by weight of the composition It is characterized by the fact that.

In addition, the composition for promoting the growth of keratinocytes, active oxygen scavenging composition, MMP, characterized in that it further comprises one or more selected from the group consisting of phosphorus extract, algae extract, gamyeo extract and Euiin extract in the sentence extract according to the present invention The composition for promoting biosynthesis of type 1 procollagen through reduction of -1 biosynthesis, and the composition for preventing skin aging are characterized in that the cosmetic composition.

Hereinafter, the present invention will be described in more detail.

The extract according to the present invention is characterized in that water is extracted with the extraction solvent, the weight ratio of the herbal medicine and the extraction solvent water during extraction is preferably 1:10 to 1:20, most preferably 1:10. This is because extraction is difficult when the mixing ratio of herbal medicine and water is less than 1:10, and the extraction efficiency decreases when the mixing ratio of herbal medicine and water is more than 1:20.

The extract according to the present invention may be used by mixing separate extracts of each herbal medicine, or after mixing the herbal medicines, it may be used to be prepared by extracting at once.

In order to obtain the herbal medicine extract of the present invention, first, each of the herbal medicines, that is, sentence-type, indong, algae, gamguk, Uiui each of the predetermined amount of water extraction solvent in a ratio of 1:10 to 1:20 by weight, preferably 1 After addition at a ratio of 10, the mixture was extracted by boiling at 60 to 100 ° C., preferably at 95 to 100 ° C., under reflux for 2 to 48 hours, preferably 18 hours, and then filtered through a filter paper to obtain the filtrate. After adding to adjust the total amount to 100 ml, the adjusting solution was left at 2 to 10 ° C., preferably 5 ° C. for 6 to 48 hours, preferably 24 hours to precipitate the low solubility in the extract components, Each herbal medicine is filtered by using a separate filtration layer, preferably Celite, which can easily catch fine substances, which are easily caught, and then membrane filtration using a 1 to 0.45 μm membrane. Extracts can be obtained.

In general, as the extraction solvent of the herbal medicine extract used as a cosmetic raw material, the same solvents as alcohols are used, but in the present invention, following the extraction method applied in Chinese medicine, the extract is heated and refluxed using water as the extraction solvent.

In order to examine the effects of the herbal extracts on skin aging, keratinocyte growth promoting effect, the active oxygen scavenging effect produced by ultraviolet light, and through the reduction of the biosynthesis of MMP-1 by ultraviolet light related to the decomposition of the skin substrate type 1 The effect of promoting the biosynthesis of procollagen was measured to confirm its efficacy, which is described in detail in the test examples below.

On the other hand, the problem when trying to apply the extract to products such as cosmetics, as a raw material is its own antiseptic ability and stability for long-term storage.

Therefore, in the present invention, the solvent composition was determined based on the solvents commonly used in cosmetics, and the solvents were dissolved in the extract by weight ratio to test skin irritation to use a minimum amount of solvent.

In the case of water extraction, since the content of polysaccharides is higher than that of solvent extraction and there is little preservative effect in water, it is not possible to avoid the generation of microorganisms when storing the extract as a raw material for a long time, so that no preservatives such as 1,3-butylene glycol If the preservative content is high, it may harm the human body or cause skin irritation, so the amount of preservative should be minimized. Therefore, as a result of several experiments to find a solvent condition having the effect of preservation, it is preferably 15 to 30 parts by weight, most preferably 15 parts by weight of 100 parts by weight of the composition containing the herbal extract and solvent according to the present invention It was found that both the preservative and the stability of the raw materials were excellent in the solvent composition containing, 3-butylene glycol.

The composition containing the extract according to the invention is characterized in that the cosmetic composition.

The cosmetic composition is a total active ingredient in the total composition, that is, extracts containing one or more extracts selected from the group consisting of phosphorus extract, almond extract, gamguk extract and Euiin extract in a concentration of 0.0001 to 25% by weight in the total composition It is preferable. This is because when the active ingredient is less than 0.0001% by weight, the effect is insignificant, and when the active ingredient is more than 25% by weight, problems in the usability of the product and the stability of the formulation occur.

In addition, the cosmetic composition may contain, in addition to the above-mentioned extracts, other components that can give a synergistic effect to the main effect, preferably within the range of not impairing the main effect of the present invention. In addition to the extract of the other ingredients can be appropriately selected and formulated by those skilled in the art according to the formulation or purpose of use of other cosmetics without difficulty.

For example, the cosmetic composition may contain a skin absorption promoting material to increase the effect. In addition, the cosmetic composition of the present invention may include a material selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

In addition, the cosmetic composition of the present invention may include other components which are usually formulated into cosmetics as needed in addition to the essential components, and examples thereof include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments. Organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

The compounding component which may be included in the cosmetic composition of the present invention is not limited thereto, and the compounding amount of the above component is possible within the range not impairing the object and effect of the present invention, but preferably 0.01 to the total weight. 10% by weight, more preferably 0.01 to 5% by weight.

The cosmetic composition according to the present invention is not particularly limited in the formulation, for example, latex, cream, lotion, essence, pack, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence Formulations such as, cleansing foams, cleansing creams, cleansing water, soaps or sprays and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

Reference Example 1 Preparation of Herbal Water Extract

Take 10 g each of Moon-Hyun, In-dong, Hang-in, Gam-guk, Ui-in (Dry Chemicals Co., Ltd.), add 100 ml of purified water, and boil for 18 hours under a reflux apparatus at about 95 to 100 ° C. The filtrate is taken and adjusted to 100 ml in total by adding water, and then the adjusting solution is left at 5 ° C. for 24 hours to precipitate the low solubility substances in the extract components and to separate the fine substances that are easily precipitated. After filtering once using a filtration layer (using Celite), each herbal medicine water extract was obtained by performing membrane filtration through 1 to 0.45 μm, and each herbal medicine water extract was prepared according to Examples 1 to 1 below. Extracts prepared by mixing in the same combination as 15 were used in the experiment. The mixing ratio of each extract was 1: 1: 1: 1. (Comparative Example 1: sentence extract alone)

 ingredient Example One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Sentence ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ Indong ○ ○ ○ ○ ○ ○ ○ ○ Passerby ○ ○ ○ ○ ○ ○ ○ ○ Country ○ ○ ○ ○ ○ ○ ○ ○ Righteous ○ ○ ○ ○ ○ ○ ○ ○

Reference Example 2 Preparation of Cosmetic Composition

Type 1 Pro through the keratinocyte growth promoting effect, the active oxygen scavenging effect produced by ultraviolet light and the biosynthesis of MMP-1 by ultraviolet light related to the decomposition of the skin substrate, according to the extract contained in Examples 1 to 15 In order to examine the effect of promoting collagen biosynthesis, the cosmetic composition of Examples 16 to 30 having the composition of Table 2 was prepared as Examples 1 to 15. The cosmetic composition prepared in Examples 16 to 30 is a composition further comprising one or more selected from the group consisting of phosphorus extract, algae extract, gamguk extract, and Euiyi extract in the sentence extract as an active ingredient, the cosmetic composition of Comparative Example 2 Is characterized by containing only fingerprint extract. The compositions were each completely dissolved in the oil phase and the water phase at 65 to 75 ℃, emulsified for 5 minutes at 7,000 to 8,000 rpm to prepare a cream formulation.

Ingredient (% by weight) Comparative Example 2 Examples 16-30 Beads wax 2 2 Stearyl alcohol 3 3 Stearic acid 8 8 Squalene 10 10 Propylene Glycol Monostearate 3 3 Polyoxyethylene ether One One Preservatives, Antioxidants Quantity Quantity glycerin 5 5 Propylene glycol 4 4 Triethylamine One One Sodium Polyacrylate 0.2 0.2 Purified water Up to 100 Up to 100 Comparative Example 1 5 - Examples 1-15 - 5 Carbomer Quantity Quantity

[Test Example 1] effect of growth promotion (MTT assay) of keratinocytes

The effect of the extract prepared in Examples 1 to 15 on the growth ability of keratinocytes was measured by the MTT method, briefly explaining the method as follows.

First, the keratinocytes were planted in a 96 well plate at a concentration of 5 × 10 ³ per 200 μl per well and incubated for 24 hours, followed by Examples 1-15 or 0.0001, 0.001, 0.01, 0.1% concentration. After treating the extract prepared in Comparative Example 1 and incubating for 48 hours, the culture solution was aspirated and removed, washed once with PBS, MTT (Methylthiazolyldiphenyl-tetrazolium bromide, Sigma, USA) (0.5 mg / ml) solution 100 μl was added to the cells and incubated at 37 ° C., 5% CO ₂ for 4 hours. Thereafter, the culture solution was aspirated and removed, 200 µl of DMSO (dimethyl sulfoxide) solution was added, shaken for 10 minutes in a shaker, and the absorbance at 540 nm was read with an ELISA reader (DIbiotech, Korea). It was. At this time, as a control group, an untreated group of the extract prepared in Examples 1 to 15 or Comparative Example 1 was used.

As a result, as shown in Table 3, it was found that the effect appears in all cases, the higher the concentration of the extract generally used, the growth promoting effect of keratinocytes also increased. In addition, the compositions of Examples 1 to 15 which further contain at least one selected from the group consisting of phosphorus extract, algae extract, gamguk extract, Euiyi extract in the sentence extract than the composition of Comparative Example 1 containing only the sentence extract is more effective, It can be seen that as the type of extract selected from the group consisting of copper extract, algae extract, gamguk extract, and extract of Euiin added to the sentence-type extract increases the growth promoting effect of keratinocytes.

% 0.0001 0.001 0.01 0.1 Example 1 138 199 243 279 Example 2 134 195 245 277 Example 3 139 200 244 274 Example 4 135 197 248 278 Example 5 140 207 256 286 Example 6 143 211 251 283 Example 7 140 216 252 282 Example 8 148 213 261 287 Example 9 142 211 254 284 Example 10 151 211 265 285 Example 11 161 225 271 291 Example 12 172 227 279 295 Example 13 166 222 272 299 Example 14 167 219 277 299 Example 15 181 236 284 333 Comparative Example 1 131 182 232 250 Control 100 100 100 100

[Test Example 2] active oxygen elimination effect

In order to determine the active oxygen generation inhibitory effect generated by ultraviolet light of the extracts prepared in Examples 1 to 15, that is, the active oxygen scavenging effect, the following experiment was performed using a fluorescent material.

First, the cell line used in the experiment was a human keratinocytes HaCaT cell line distributed from Dr. Fusenig of the German Cancer Research Center, which is a 96 well black plate for fluorescence measurement. Dispense at 2.0 × 10 ⁴ per well and add penicillin / streptomycin to DMEM (Dulbeccos Modification of Eagles Medium, FBS 10%, Gibco, USA) media at 37 ° C., 5% CO ₂ conditions. After culturing for one day, the extracts prepared in Examples 1 to 15 or Comparative Example 1 were treated at concentrations of 0.5, 0.25, 0.125, 0.0625 and 0.03125%.

After the test sample was incubated for 24 hours and washed with HCSS (HEPES-buffered control salt solution, Gibco, USA), the remaining medium was removed, and DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate prepared in 20 μM in HCSS) was removed. , MolecularProbes, USA) was added to 100ul and incubated for 20 minutes at 37 ℃, 5% CO ₂ conditions and washed with HCSS. After 100 μl of HCSS treated for each olfactory sample concentration, the fluorescence of DCF (dichlorofluorescein) oxidized with active oxygen was measured with a fluorescence plater (Ex = 485 nm, Em = 530 nm). After UVB (30mJ / cm ² ) was irradiated and measured immediately after the treatment (Table 4) and 3 hours after the treatment (Table 5) by using a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). At this time, as a control group, an untreated group of the extract prepared in Examples 1 to 15 or Comparative Example 1 was used.

As a result, as shown in Table 4 and Table 5, it can be seen that the effect appears in all cases, the higher the concentration of the extract generally used, the more active oxygen production was found to be more suppressed. In addition, the compositions of Examples 1 to 15 further contain at least one selected from the group consisting of phosphorus extracts, almond extracts, gamguk extract, Uyiin extracts than the composition of Comparative Example 1 containing only the sentence extracts, It can be seen that the more active kinds of extracts selected from the group consisting of phosphorus extracts, almond extracts, gamguk extracts, and extracts of Ui-in extracts added to the sentence-type extracts, the more active oxygen is also inhibited accordingly. It was also found that the effect was higher after 3 hours of treatment than immediately after treatment.

% 0.5 0.25 0.125 0.0625 0.03125 Example 1 71.55 75.71 75.71 82.71 82.72 Example 2 71.39 75.66 75.72 82.72 82.71 Example 3 71.45 75.51 75.62 82.65 82.71 Example 4 71.38 75.61 75.61 82.71 82.70 Example 5 71.43 75.28 75.30 82.51 82.62 Example 6 71.38 75.20 75.31 82.42 82.61 Example 7 71.39 75.31 75.42 82.50 82.62 Example 8 71.39 75.21 75.31 82.32 82.52 Example 9 71.41 75.34 75.51 82.33 82.51 Example 10 71.43 75.25 75.50 82.41 82.61 Example 11 71.05 74.65 75.03 81.34 82.01 Example 12 70.97 74.52 76.85 81.34 81.96 Example 13 71.01 74.62 75.00 81.31 81.85 Example 14 70.84 74.45 75.01 81.30 81.85 Example 15 70.50 73.55 74.70 80.30 81.21 Comparative Example 1 72.50 76.71 76.36 83.71 83.65 Control 100 100 100 100 100

% 0.5 0.25 0.125 0.0625 0.03125 Example 1 61.01 68.97 73.61 80.84 80.00 Example 2 61.01 68.87 73.54 80.22 79.96 Example 3 61.02 69.01 73.60 80.65 80.00 Example 4 61.00 69.00 73.61 80.67 79.95 Example 5 60.95 69.00 73.02 80.52 79.94 Example 6 61.04 68.95 72.97 80.52 79.92 Example 7 60.95 68.97 73.21 80.51 80.00 Example 8 60.97 69.00 72.82 80.30 79.97 Example 9 61.00 69.00 73.64 80.41 80.00 Example 10 60.96 69.00 73.01 80.25 79.96 Example 11 60.51 68.62 71.61 79.50 79.95 Example 12 60.41 68.41 71.42 79.73 79.95 Example 13 60.25 68.52 71.41 79.71 79.94 Example 14 60.34 68.42 71.52 79.66 79.94 Example 15 59.42 66.26 71.06 78.61 78.90 Comparative Example 1 61.50 69.99 73.97 81.85 80.97 Control 100 100 100 100 100

Test Example 3 MMP-1 and Procollagen Assay

MMP-1 and procollagen analysis to determine the effect of reducing the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of the skin substrate of the extract prepared in Examples 1 to 15 to promote the biosynthesis of type 1 procollagen ( procollagenassay) was performed in the following manner.

First, the fat layer of the normal epidermis is removed and chopped to separate the epidermis and dermis with collagenase, and then the epidermis and dermis are placed in 0.25% trypsin solution, respectively, at 37 ° C. and 5% CO ₂ incubator for 10 minutes. Treated. Since vortex was performed to release the keratinocytes and fibroblasts, respectively. After collecting and washing the free cells, keratinocytes were cultured at 1 × 10 ⁴ cells / cm ² in KGM (keratinocyte growth medium, Clonetics, USA), and fibroblasts were added with 10% fetal bovine serum (FBS). Cultured in DMEM medium. When grown at about 70-80%, the cells were subcultured at a ratio of 1: 3, and cells subjected to 3-4 passages were used for the experiment.

For experiments to measure the amount of MMP-1, fibroblasts were cultured in at least 90% in 48 well plates and then starved for one day. After washing twice with PBS, the lid of the plate (plate) was opened with 100 μl of PBS, and UV A (Dermlightcube 401 equipped with UVA filters, UVAtec, USA) was irradiated with 15 J / cm ² . Immediately after irradiation, washed again with PBS once, and added DMEM without fetal bovine serum containing 0.001 and 0.01% of the extract prepared in Examples 1 to 15 or Comparative Example 1, respectively, at 37 ° C. and 5% CO ₂ incubator. After 48 hours of incubation, the amount of free MMP-1 in the medium was measured using the MMP-1 human ELISA system (RPN2610, Amersham Pharmacia Biothch, UK) and corrected for the total protein amount of fibroblasts. (Table 6). At this time, as a control group, an untreated group of the extract prepared in Examples 1 to 15 or Comparative Example 1 was used.

For experiments to measure the amount of procollagen, extracts prepared in Examples 1 to 15 or Comparative Example 1 were added without starvation and ultraviolet irradiation under the same conditions, respectively, and after 24 hours, The amount was measured using the procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan) (Table 7). As a control group, an untreated group of the extract prepared in Examples 1 to 15 or Comparative Example 1 was used.

As a result, as shown in Table 6 to the effect of inhibiting MMP-1, it can be seen that the effect appears in all cases, the higher the concentration of the extract generally used, the amount of MMP-1 produced is reduced. Could. In addition, the compositions of Examples 1 to 15 further contain at least one selected from the group consisting of phosphorus extracts, almond extracts, gamguk extract, Uyiin extracts than the composition of Comparative Example 1 containing only the sentence extracts, As the type of extract selected from the group consisting of phosphorus extracts, almond extracts, gamguk extracts, and extracts of euphoric extracts added to the sentence-type extracts, it was found that the amount of MMP-1 produced decreased accordingly.

In addition, as shown in Table 7 below to increase the biosynthesis of type 1 procollagen, it was found that the effect appears in all cases, the higher the concentration of the extract generally used, the higher the amount of procollagen produced Could know. In addition, the compositions of Examples 1 to 15 further contain more than one selected from the group consisting of phosphorus extract, almond extract, gakguk extract, Uyiin extract in the sentence extract than the composition of Comparative Example 1 containing only the sentence extract, It can be seen that the amount of procollagen produced increases according to the type of extract selected from the group consisting of copper extract, algae extract, Korean extract, and Euiin extract added to the sentence extract.

% 0.001 0.01 Example 1 92 76 Example 2 91 74 Example 3 89 72 Example 4 91 75 Example 5 85 61 Example 6 81 56 Example 7 83 61 Example 8 81 57 Example 9 82 60 Example 10 72 53 Example 11 72 52 Example 12 73 52 Example 13 71 53 Example 14 74 54 Example 15 70 50 Comparative Example 1 95 79 Control 100 100

% 0.001 0.011 Example 1 106 118 Example 2 108 119 Example 3 105 116 Example 4 109 111 Example 5 110 123 Example 6 114 124 Example 7 116 124 Example 8 115 122 Example 9 107 125 Example 10 126 136 Example 11 127 135 Example 12 125 131 Example 13 127 131 Example 14 123 132 Example 15 133 141 Comparative Example 1 101 105 Control 100 100

[Test Example 4] Wrinkle improvement effect

In order to see the wrinkle improvement effect of the extract prepared in Examples 16 to 30 or Comparative Example 2 was performed as follows.

After dividing 140 women in their thirties into seven groups of 20 people each, the cream of Example 30 or Comparative Example 2 was applied to the designated eye area twice a day for eight weeks each morning, and then a replica replica of silicon was produced. The condition of wrinkles at the designated sites was measured by a visometer (SVI60, Courage + Khazaka electronic GmbH, Germany). As a control, an untreated group of creams of Examples 16 to 30 or Comparative Example 2 was used. The results are shown in Table 8, which shows the average of each parameter value after 8 weeks minus the parameter value 8 weeks ago. In other words, the negative the value, the higher the wrinkle improvement effect.

division R1 R2 R3 R4 R5 Example 30 -0.25 -0.19 -0.10 -0.09 -0.07 Comparative Example 2 -0.10 -0.07 -0.04 -0.03 -0.03 Control 0.28 0.27 0.22 0.04 0.04 R1: The difference between the highest and lowest values of the wrinkled contours R2: The randomly divided wrinkles contours by 5 spaces Rn: Average of R1 values divided by 5 R1: The highest value of R1 values divided by 5 R4: The top of each line at the baseline of the wrinkled contours R5 minus values of and valleys R5: Mean value of base line of wrinkle contour line minus each wrinkle profile

As shown in Table 8, the skin wrinkle improvement effect of the composition of Example 30, which contains all of the phosphorus extract, almond extract, gamguk extract and Euiin extract in the sentence extract compared to the composition of Comparative Example 2 containing only the sentence extract, Could be confirmed.

Examples of the formulation of the composition will be described below, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Soap Preparation Examples 1-15 Soap Preparation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 1.00 (%) maintain A reasonable amount Sodium hydroxide A reasonable amount Sodium chloride A reasonable amount Spices Small quantity

The total amount was 100 with purified water, and soap was prepared according to the blending ratio (% by weight).

[Lotion Formulation Examples 1-15] Lotion Preparation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 3.00 L-ascorbic acid-2-magnesium phosphate 1.00 Water Soluble Collagen (1% Aqueous Solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Licorice Extract 0.20 1,3-butylene glycol 3.00

The whole amount was 100 with purified water, and a lotion was prepared by the above compounding ratio (% by weight).

[Cream Formulation Examples 1-15] Cream Preparation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 1.00 Polyethylene Glycol Monostearate 2.00 Self-emulsifying glycerin monostearate 5.00 Cetyl alcohol 4.00 Squalene 6.00 Glyceryl tri2-ethylhexanoate 6.00 Sphingolipid 1.00 13, butylene glycol 7.00

The total amount was 100 with purified water, and a cream was prepared at the blending ratio (% by weight).

[Pack Formulation Examples 1-15] Pack Preparation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 5.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-magnesium phosphate 1.00 Lauroylhydroxyproline 1.00 Water Soluble Collagen (1% Aqueous Solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00

The whole amount was 100 with purified water, and a pack was prepared at the above compounding ratio (% by weight).

[Solution Formulation Examples 1 to 15] Cosmetic Solution Preparation

ingredient Content (% by weight) Each of the extracts of Examples 1-15 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 13-butylene glycol 6.00 Dark glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00

The whole amount was 100 with purified water, and a cosmetic liquid was prepared in the above blending ratio (wt%).

As described above, the extract of the present invention further comprises at least one selected from the group consisting of phosphorus extracts, almond extracts, gamguk extracts and Uiin extracts in the sentence-type extract to the growth promoting effect of keratinocytes, UV The active oxygen scavenging effect, the biosynthesis of MMP-1 by ultraviolet rays related to the decomposition of the skin substrate, thereby promoting the biosynthesis of type 1 procollagen and the anti-aging effect. It can be usefully used in cosmetics as a composition for promoting cell growth, a composition for scavenging free radicals, a composition for promoting biosynthesis of type 1 procollagen through a decrease in biosynthesis of MMP-1, and a composition for preventing skin aging.

Claims (7)

Cosmetic composition for promoting the growth of keratinocytes, characterized in that it further comprises at least one selected from the group consisting of phosphorus extract, algae extract, gamguk extract and Euiin extract as a active ingredient. The active ingredient scavenging cosmetic composition, characterized in that it further comprises one or more selected from the group consisting of phosphorus extract, algae extract, gamguk extract and Uiin extract as an active ingredient. Cosmetic composition for promoting the biosynthesis of type 1 procollagen through reducing the biosynthesis of MMP-1, characterized in that it further comprises at least one selected from the group consisting of phosphorus extract, almond extract, gamguk extract and Uuiin extract as a active ingredient Composition. The composition for preventing skin aging, characterized in that it further comprises one or more selected from the group consisting of phosphorus extract, almond extract, gamguk extract and uiyiin extract as an active ingredient. The composition according to any one of claims 1 to 4, wherein the extract is extracted using water as an extraction solvent. The composition of claim 5, wherein the composition further contains 1,3-butylene glycol. The cosmetic composition according to any one of claims 1 to 4, wherein the extract contains 0.0001 to 25% by weight based on the total weight of the total composition.