KR101142541B1 - Anti-wrinkle cosmetic composition containing oriental herb extract treated by enzyme and its extraction method - Google Patents

Abstract

The present invention relates to a mixed medicinal herb extract which is obtained by mixing Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong and Gamsohyeong at a certain ratio and extracting them by enzyme treatment and a cosmetic composition for improving skin wrinkles using the same. The cosmetic composition for improving skin wrinkles containing an extract of herbal medicine is very effective for improving the wrinkles of the skin by exerting an excellent effect in antioxidation, inhibition of elastase expression and improvement of skin wrinkle in clinical evaluation, It is very safe because it has no side effects.

Description

[0001] The present invention relates to a cosmetic composition for improving skin wrinkles containing an extract of mixed herbal medicines using an enzyme treatment and an extracting method thereof,

The present invention relates to a cosmetic composition for improving skin wrinkles containing an extract of mixed herbal medicines of Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong and Gamsohyeong. More particularly, the present invention relates to a mixed medicinal herb extract which is obtained by mixing Baekbuja, Baekji, Youngryungbyeol, Hyangbuja, Baekbokryeong and Gamsohyeong at a certain ratio and extracting them by enzyme treatment and a cosmetic composition for improving skin wrinkles using the same. The mixed herbal medicine extract and the cosmetic composition for improving skin wrinkles are safe because they have excellent skin wrinkle-improving effect and have no skin irritation and side effects when used.

Because the skin has many opportunities to interact with various external stimuli, it is more prone to wrinkles than other organs. Among them, facial skin is directly exposed to sunlight, dry outside air and pollutants, so that aging phenomenon such as wrinkles occurs more early than other skin.

The most characteristic change due to aging of the skin tissue is a change in the skin matrix, which causes aging of the human skin fibroblast in the dermis, resulting in a decrease in the ability to produce fibrous and matrix. The amount of the substrate is generally decreased, the thickness of the skin is thinned, and the elasticity of the skin is lowered to form the wrinkles. That is, as aging progresses, the skin becomes weaker in elasticity, disorder of blood circulation, weakening of skin barrier, and the like.

In addition, exposure of the skin to ultraviolet rays produces free radicals and reactive oxygen species, the main causes of skin cell damage. They can cause damage to the DNA, attack cell membrane structures, generate aging spots, and accelerate wrinkle formation by attacking collagen and fibers that keep skin moist, soft and supple and elastic.

Many cosmetics have been studied to reduce skin wrinkles caused by such external environment or internal mechanism. Recently, in the development of cosmetics, a natural herbal medicine using pure natural oriental herbal medicine which provides natural nutrition to skin by using natural products, minimizes toxic effects of cosmetics on skin by minimizing side effects while also treating various symptoms of skin Interest in the development of cosmetics is growing.

As described above, various natural materials and cosmetic compositions have been developed for the purpose of wrinkling the skin and preventing aging. For example, Korean Patent No. 10-0722955 entitled " Composition Containing Anti-Skin Aging and Anti-Wrinkle " describes a composition comprising a topical extract obtained by extracting the upper leaves with methanol, a Gujia extract extracted with water, In a weight ratio of 40 to 70: 5 to 20: 10 to 50, respectively, and a Korean Patent Registration No. 10-07531860 entitled " A cosmetic composition for preventing skin aging characterized by containing an extract of herbal medicine comprising an extract of Hassuo, Baekbokryeong, Wusul, Angelica gigas, Gujia, Tosa, and Ginseng as an effective ingredient in a cosmetic composition for preventing skin aging.

In addition, Korean Patent Application No. 10-2004-0106688 entitled " Cosmetic Composition Containing Traditional Herbal Medicines Improving Skin Wrinkles ", which uses herbal medicine extract as an active ingredient, And a cosmetic composition in the form of a softening agent, a nutritional lotion, a nutritional cream, a massage cream, an essence, and a pack having an effect of improving skin wrinkles. Korean Patent Application No. 10-2006-0103224 "Antioxidant, The present invention relates to an invention of a makeup composition for the elderly which shows a whitening effect, a wrinkle improvement effect and an anti-aging effect, A composition containing a mixed herbal extract comprising jujubes, ginseng, green tea, and shiitake mushrooms is disclosed.

The Whitefish ( Aconitum koreanum Raymond) is also known as the yellow hinge, a perennial plant belonging to the Ranunculaceae , the root of the rich. The major components of the white centaur are known as aconitine, atisine, higenamine, coryneine, and yokonoside, And analgesia. Korean Patent No. 10-0785505 discloses a herbal medicine extract having an antioxidative effect and a whitening effect as a cosmetic composition including a white marble, and a method for producing the extract.

Angelica dahurica Bentham et Hooker is a perennial root of the perennial plant belonging to the genus Umbelliferae . The major components of the white paper are known as coumarin, such as oxypeucedamin, byakangelicol, xanthotoxin, and the like. In the oriental medicine, headache, nasal congestion, And is effective for gastrointestinal disorders, postpartum headache, dizziness, toothache, facial neuralgia, paralysis. In addition, when menstrual bleeding, fever, and feces are mixed with blood, headache due to empyema, rejuvenation, poisoning, and skin ulcers are also effective. In addition, antimicrobial activity by pharmacological action, animal aging vascular exercise center, respiratory center, excitability of vagus nerve and spinal cord part, coronary artery blood flow increasing action, white wind wind (white spot wind) : Chronic dermatosis, a disease in which multiple layers of white scale powder are formed on the skin surface due to erythema and papules) has been reported to be effective. Korean Patent No. 10-0272463 entitled " Extracts Extracted from Natural Products for Use in Cosmetics ", No. 10-0820237, " Cosmetic Composition Comprising Combined Herbal Extracts Showing Antioxidant and Humectant Activity " -0525994 " Cosmetic Composition Containing Dextran Herbal Extract " and the like are disclosed.

Liliaceae ( Lysimachia foenum-graecum Hance) is a biennial plant of Primulaceae . Its stem is about 70cm in height. Leaves are alternate and compound leaf. In summer, flower stem comes out from leaf axil, It is used when there is a headache due to a cold caused by a cold flower. It treats the symptoms of bloating of the chest with fragrance. It is known that Europe has its origin in Korea and has analgesic, anti-inflammatory and whitening effects in oriental medicine. Korean Patent No. 10-0272463 discloses an extract derived from a natural product for use in cosmetics.

Cyperus rotundus Linne is a perennial herbaceous roots of Cyperaceae , and its major components are α-cyperone, cyperene, and cyperol. Anti-inflammatory, antimicrobial, and antioxidant effects are known in oriental medicine. Korean Patent No. 10-0537960 entitled " Cosmetic Composition Containing Extract of Herbal Medicines and Its Extraction Method ", Japanese Patent JP2002-29923 " Collagen Production Promoter, Escherichia Inhibitor, Collagen Activity Inhibitor and Skin Cosmetic Ingredient " .

Poria cocos Wolf is a sclerot of the genus Bombyx mori, an herb belonging to Polyporaceae . Pachyman, a polysaccharide of glucose, and pachymic acid, a trutherphenoid family, are known to be effective ingredients of Baek Bok-Yeong, and the effects of hemicidal action, antibacterial action and hypoglycemic effect are known. Korean Patent No. 10-0545044 entitled " Cosmetic composition containing herbal composition complex extract stabilized with nanoliposome ", No. 10-0753186 " Cosmetic composition for preventing skin aging containing herbal extract mixture " .

Gamseonghyang (Nardostachys chinensis Batal) is the root of ginseng, an herbaceous plant belonging to the Valerianaceae family. The major components of ginseng extract are nardostachnol, aristolen, and jatamansin, and the effects of anti-inflammatory, antimicrobial, and antioxidant effects are known in Oriental medicine . Korean Patent No. 10-0546735 " Skin Whitening Agent Composition ", Japanese Patent Application No. 10-0858438 " Composition of skin cosmetic pack "

In the present invention, the herbal medicine, herbaceous shrub, Korean herb scent, herbal vertebra, Baekbokryeong, and ginseng shale are searched for herbal medicinal herb medicinal materials effective for preventing wrinkles and aging of the skin and the enzymatic treatment process is applied to the selected herbal medicines, The present inventors have developed a mixed herbal extract having improved antioxidative and anti-wrinkle effects as compared with the herbal extracts of the prior art.

Accordingly, the technical problem to be solved by the present invention is to provide a cosmetic composition which is effective for inhibiting antioxidant and elastase activity which tests skin wrinkle improvement without causing side effects of skin, and which is useful for improving skin wrinkles.

Accordingly, an object of the present invention is to provide a process for the second purification of the first extract obtained from the mixed herb medicine of Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryong, The present invention provides a cosmetic composition and a cosmetic composition using the cosmetic composition, which comprises an extracted component as an active ingredient.

A method for separating and extracting an effective ingredient for cosmetic provided to achieve the object of the present invention comprises the steps of (a) preparing a mixed herbal medicine by mixing and grinding Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong, (B) adding an extraction solvent to the mixed herbal medicines, and then extracting the mixed herbal medicines by an extraction process; (c) subjecting the crude herbal medicinal extract to enzymatic treatment to obtain a mixed herbal extract; And then proceeding to purification.

In the method according to the present invention, the content of each of the white ginseng, white ginseng, Korean ginseng, herbal ginseng, Baekbokwol and ginseng shale contained in the mixed herbal medicine prepared in the step (a) is 10 to 40 wt% desirable.

In addition, in the method according to the present invention, the extraction solvent to be added to the herbal medicine in the step (b) may be any extraction solvent known in the art, and preferably purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms , A pure solvent selected from the group consisting of ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol, or a combination solvent of two or more thereof is used, It is preferable that the amount is 1 to 20 times.

Meanwhile, the extraction process performed in the step (b) may be a general extraction process known in the art using an extraction solvent. Preferably, the extraction process is carried out at a temperature of 5 to 37 ° C for 1 to 15 days by immersion extraction Or by mounting the cooling condenser and then heating the mixture at a temperature of 50 to 95 DEG C for 4 to 20 hours.

Further, in the method according to the present invention, in the step (c), the enzyme treatment of the crude herbal medicine crude extract is performed by concentrating the crude extract to remove the extraction solvent, adding purified water containing 1 to 10% Dispersed in a solvent, added with 1 to 10% by weight of enzyme to the total solvent, and treated at 50 ° C for 12 hours.

The enzyme used in step (c) may be one kind of enzyme selected from carbohydrase and pectinase, or a complex enzyme in which these enzymes are mixed.

As the carbohydrate hydrolase, Viscozyme ^® L (Carbohydrase, 100 FBG / g: Novozymes, Denmark) and Celluclast ^® 1.5 LFG (Cellulase, 700 EGU / g: Novozymes, Denmark) can be used. As the pectin hydrolyzing enzyme Pectinex ^® 100L (Pectinase, 5,000 FDU / mL: Novozymes, Denmark) can be used.
As described above, the cosmetic composition capable of improving skin wrinkles according to the present invention can be obtained by extracting mixed herbal medicines of Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong, And extracting the mixed herbal medicines.

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In order to achieve the other object of the present invention, the cosmetic composition of the present invention is obtained by mixing the mixed herbal extracts extracted from the mixed herbal medicines of Baekbuja, Baekji, Youngryungbyeo, By weight.

In the present invention, the term " effective ingredient " refers to a substance or a group of substances (pharmacologically active ingredients, etc., which are expected to directly or indirectly express the efficacy or efficacy of the drug by its inherent pharmacological action As used herein, including the main component.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

Dosage forms of the pharmaceutical compositions of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

The cosmetics prepared using the mixed herbal medicine extract of the present invention can be prepared and used in formulations such as cosmetics for basic cosmetics or makeup, essences, creams, lotions, packs, foundations, makeup bases and the like . It can be applied in various forms such as liquid, cream, paste, and solid, and can be manufactured by a conventional method of making cosmetics. Cosmetic preparations prepared by the various formulations have functional properties that can be used for improving skin wrinkles.

As described above, the present invention provides a cosmetic composition comprising an enzyme-treated mixed herbal extract of Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong, and Sanshin. The cosmetic composition according to the present invention is a natural product and has no skin side effects, is excellent in antioxidative and elastase activity inhibitory effects related to the improvement of wrinkles of the skin, and is thus useful for improving the wrinkles of the skin.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited to these embodiments.

≪ Examples 1 to 5 >

As shown in Table 1, 50 g of the mixed herbal medicines mixed at the respective weight percentages were put into an extractor, and then the mixture ratio of ethanol and purified water was adjusted to the mixing ratio of each of ethanol, purified water, Then, 500 mL of the extraction solvent was added, and the mixture was refluxed and extracted twice for 4 hours. The extracted filtrate was filtered with a filter paper of No. 2 Watt, and the filtrate was concentrated under reduced pressure using a rotary evaporator to completely remove the solvent. Then, the filtrate was dissolved in 250 mL of a 40% by weight aqueous solution of butyleneglycolic acid to obtain a crude herbal extract.

division Content (g) Example 1 Example 2 Example 3 Example 4 Example 5 A white man 10 10 10 10 10 blank 10 10 10 10 10 Youngrung incense 10 10 10 10 10 Herbalist 10 10 10 10 10 Baekbokryung 5 5 5 5 5 Gam-cho 5 5 5 5 5 ethanol 500 375 250 125 - Purified water - 125 250 375 500

≪ Examples 6 to 11 &

As shown in Table 2, 50 g of mixed herbal medicines mixed at the respective weight percentages were put into an extractor, and then mixed according to the mixing ratios of ethanol and purified water, respectively, 500 mL of extraction solvent was added, and the mixture was refluxed and extracted twice for 4 hours each time. The extract was filtered through a No. 2 filter paper of Watts to obtain a crude extract of a primary herbal medicine. The mixed crude herbal extract was concentrated under reduced pressure using a rotary evaporator to completely remove the solvent, dissolved in 500 mL of a 10 wt% ethanol solution, 15 mL of each selected enzyme was added, and the mixture was incubated in a shaking incubator (shaking incubator) for 12 hours. The enzyme-treated mixed crude extract was filtered with filter paper No. 2, and the filtered extract was further concentrated under reduced pressure using a rotary evaporator to remove the solvent. The aqueous solution of 40% by weight of butylene glycol dissolved in 250 mL And then filtered again to obtain a mixed herbal extract.

division Content (g) Example 6 Example 7 Example 8 Comparative Example 1 Example 9 Example 10 Example 11 Comparative Example 2 A white man 10 10 10 10 10 10 10 10 blank 10 10 10 10 10 10 10 10 Youngrung incense 10 10 10 10 10 10 10 10 Herbalist 10 10 10 10 10 10 10 10 Baekbokryung 5 5 5 5 5 5 5 5 Gam-cho 5 5 5 5 5 5 5 5 Purified water 375 375 375 375 250 250 250 250 ethanol 125 125 125 125 250 250 250 250 Viscozyme L 15 - - - 15 - - - Pectinex 100L - 15 - - - 15 - - Celluclast 1.5LFG - - 15 - - - 15 -

≪ Comparative Examples 1 and 2 &

In the above-described Examples 6 to 11, the same method as that of the extraction using the enzyme treatment was carried out, but the mixed herbal extract without enzyme treatment was prepared and compared with Examples 6 to 11.

≪ Comparative Examples 3 to 8 &

As shown in Table 3, 50 g of each selected medicinal material was placed in an extractor, and 500 mL of extraction solvent was added to the extractor according to the mixing ratio of ethanol and purified water, respectively, Was added, and the mixture was cooled twice by repeatedly refluxing for 4 hours each time. The extracted filtrate was filtered with a filter paper of No. 2 watt, and the filtrate was concentrated under reduced pressure using a rotary evaporator to completely remove the solvent. Then, the filtrate was dissolved in 250 mL of a 40 wt% aqueous solution of butyleneglycolic acid to obtain a single herbal extract.

division Content (g) Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 A white man 50 - - - - - blank - 50 - - - - Youngrung incense - - 50 - - - Herbalist - - - 50 - - Baekbokryung - - - - 50 - Gam-cho - - - - - 50 Purified water 375 375 375 375 375 375 ethanol 125 125 125 125 125 125

≪ Experimental Example 1 >

The free radical scavenging activity test was conducted to examine the antioxidative effect of the herbal extracts of the present invention prepared as the active ingredient of the cosmetic according to the present invention. The free radical scavenging test was a modification of the method of Kim et al . ( Kor. J. Pharmacogn. , 24 (4), 299-303, 1993), and a stable free radical, DPPH (1,1-diphenyl- Sigma) reagent was used.

First, 1 mL of a 0.2 mM DPPH solution was diluted with methanol to a suitable concentration in each of the samples obtained according to the above extraction examples, and 2 mL was mixed. After allowing to stand at room temperature for 10 minutes, the absorbance was measured at 517 nm. In the meantime, the control group in the free radical scavenging test was set to add methanol instead of the sample solution, measure the same method, and add methanol instead of the DPPH solution to obtain the color correction value for each of the examples.

The free radical scavenging ratio was calculated numerically using the following equation (1). In the following Tables 3 and 4, SC ₅₀ is a concentration of a sample required to achieve a free radical scavenging ratio of 50%, which is a value used in comparison with other constituent materials. The smaller the value, the higher the scavenging rate. Experiments were carried out three times each and expressed as mean ± standard deviation.

The active oxygen scavenging test is a modification of the method of Noro et al. ( Chem. Pharm. Bull ., 31, 3984-3987, 1983). The scavenging test of active oxygen (superoxide anion) The change of absorbance by oxidation of nitroblue tetrazolium (NBT) by reactive oxygen species was measured using an active oxygen generating system by enzyme reaction. Add 2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA, 0.1 mL of BSA, 0.1 mL of NBT, and 0.1 mL of the sample, mix well, and leave at 25 ° C for 10 minutes. Xanthine oxidase (0.1 mL) was added and incubated at 25 ° C for 20 minutes. The reaction was stopped by adding 6 mM CuCl ₂ and absorbance was measured at 560 nm. Meanwhile, in the control group in the active oxygen scavenging test, purified water was added in place of the sample solution, and the same method was used to measure the color correction value for each of the examples by adding purified water instead of xanthine oxidase solution.

The active oxygen scavenging rate was calculated using the following equation (2), and the results are shown in the following Tables 3 and 4. Experiments were carried out three times each, and expressed as a mean value ± standard deviation. In the following Tables 3 and 4, the IC ₅₀ is a concentration of a sample required to achieve an active oxygen scavenging rate of 50%, which is a numerical value used in comparison with other component materials. The smaller the value, the higher the erasure rate.

                         Absorbance of control - absorbance of each extract

Free radical scavenging rate (%) = --------------------------------------- X 100

                                  Absorbance of control group

                         Absorbance of control - absorbance of each extract

Ratio of active oxygen scavenging (%) = --------------------------------------- X 100

                                  Absorbance of control group

division SC ₅₀ ([mu] L / mL) IC ₅₀ ([mu] L / mL) Example 1 19.479 ± 0.660 225.944 + 4.146 Example 2 10.721 + - 0.516 97.949 + 0.684 Example 3 8.927 + 0.073 102.422 + 1.090 Example 4 8.642 + 0.194 130.602 + 2.776 Example 5  10.566 ± 0.549 141.729 ± 1.083

As shown in the results shown in Table 4, in the results of the free radical scavenging test, in Examples 1 to 5, Examples 3 and 4, which were extracted using a 50 wt% aqueous ethanol solution and a 25 wt% 5 showed better scavenging activity. On the other hand, in the results of the active oxygen scavenging test, the active oxygen scavenging activity of Examples 2 and 3, which were extracted using an aqueous solution of 75 wt% and 50 wt% ethanol, showed relatively good active oxygen scavenging activity.

On the basis of the above results, it was confirmed that the extraction methods using the above-described enzymatic treatment using the 50 wt% ethanol aqueous solution and the 25 wt% ethanol aqueous solution were the same as those of Examples 6 to 11 and Comparative Examples 1 and 2, 8, and the free radical scavenging test and the reactive oxygen scavenging test were carried out in the same manner. The results are shown in Table 5 below.

division SC ₅₀ ([mu] L / mL) IC ₅₀ ([mu] L / mL) Example 6 8.223 + 0.040 53.579 ± 1.532 Example 7 9.606 + - 0.232 68.978 ± 1.394 Example 8 9.456 ± 0.186 110.599 + 1.451 Example 9 9.304 + 0.211 68.941 ± 1.007 Example 10 10.298 + 0.129 68.464 ± 1.787 Example 11 10.168 ± 0.068 105.225 ± 3.187 Comparative Example 1 12.030 + 0.088 172.143 ± 1.929 Comparative Example 2 14.571 + 0.368 137.089 ± 1.772 Comparative Example 3 16.720 ± 0.421 420.344 ± 1.583 Comparative Example 4 35.484 ± 1.128 - Comparative Example 5 18.182 + - 0.551 632.344 ± 1.352 Comparative Example 6 12.012 + 0.648 921.468 ± 6.745 Comparative Example 7 - - Comparative Example 8 13.333 + 0.408 808.371 ± 1.966

As shown in Table 5, free radical scavenging test showed relatively good scavenging activity in Examples 6 to 11 as compared with Comparative Examples 1 and 2 in which no enzyme was used. Also, as shown in the free radical scavenging test results, Examples 6 to 11 showed relatively good scavenging activity as compared with Comparative Examples 1 and 2. In particular, it was confirmed that the scavenging activity of Examples 6 and 9 prepared by treating Viscozyme L among the enzymes was relatively increased. In addition, it was confirmed that the effectiveness of Examples 6 to 11, which are enzyme-treated mixed herbal extracts of the present invention, was significantly improved as compared with the free radical scavenging activity and the active oxygen scavenging activity of Comparative Examples 3 to 8, which are herb extracts.

≪ Experimental Example 2 >

In order to confirm the wrinkle-improving effect of the mixed herbal medicine extracts prepared in the above examples as an active ingredient material of the cosmetic according to the present invention, the inhibition of activity against elastase was measured.

N-Succinyl-ala-ala-ala-ρ-nitroaniline (Sigma), an elastase substrate, was used to measure the activity of elastase, an enzyme that degrades elastin. The change in color is measured by measuring the absorbance at a wavelength of 405 nm to measure the elastase activity. The buffer solution was 0.267 M Tris-HCl (pH 8.0, Sigma), the substrate solution was 8.8 mM N-Succinyl-ala-ala-ala-ρ-nitroaniline and the enzyme solution was incubated with 10 μg / Respectively. After mixing 60 μL of buffer, 20 μL of substrate and 100 μL of each sample diluted to 1 mg / mL, 20 μL of the enzyme solution was added and reacted in a constant-temperature water bath at 25 ° C. for 15 minutes to produce ρ-nitroaniline Plate reader at a wavelength of 405 nm.

In the control group for the wrinkle-improving effect test, purified water was added in place of the sample solution, and the same colorimetric values were obtained for the sample and the control group by adding the purified water in place of the enzyme solution. The inhibitory effect of elastase activity was numerically calculated using the following equation (3), and the results are shown in Table 6 below. Experiments were carried out three times each, and expressed as a mean value ± standard deviation. In Table 6, IC ₅₀ is the concentration of the sample required to achieve the inhibition rate of the elastase activity of 50%, which is a value used in comparison with other component materials. The smaller the value, the higher the erasure rate.

                               Absorbance of control - absorbance of each extract

Elastase activity inhibition rate (%) = --------------------------------------- X 100

                                        Absorbance of control group

division IC ₅₀ ([mu] L / mL) Example 1 697.086 ± 6.053 Example 2 580.301 + 1.192 Example 3 552.062 + - 4.954 Example 4 513.692 ± 3.241 Example 5 442.427 ± 3.096 Example 6 399.063 ± 6.365 Example 7 369.512 8.378 Example 8 323.857 + - 3.524 Example 9 441.621 + - 3.091 Example 10 461.340 ± 17.352 Example 11 397.229 + - 4.056 Comparative Example 1 546.911 + - 4.231 Comparative Example 2 562.386 ± 4.958 Comparative Example 3 (inhibition rate,%) 25.622 + - 0.660 Comparative Example 4 (inhibition rate,%) 30.345 + 0.406 Comparative Example 5 (inhibition rate,%) 41.185 ± 0.226 Comparative Example 6 (inhibition rate,%) 32.799 + 2.014 Comparative Example 7 (inhibition rate,%) 17.156 ± 0.926 Comparative Example 8 (inhibition rate,%) 10.939 + 0.375

As can be seen from the results shown in Table 6, in Examples 6 to 11 in which the enzyme treatment was applied, the effect of inhibiting the elastase activity was relatively increased as compared with Examples 1 to 5 and Comparative Examples 1 and 2 in which the enzyme treatment was not applied I could confirm. In addition, in the case of the herbal medicine extracts of Comparative Examples 3 to 8, the IC ₅₀ , which is the concentration of the sample required to achieve the inhibition rate of the elastase activity of 50%, did not appear, and the concentration of 10-40% , The effectiveness of the enzyme-treated mixed herbal extracts, Examples 6 to 11, was remarkably improved.

≪ Formulation Example 1 and Comparative Formulation Example 1 >

Ingredients of the nutritional skins containing the mixed herbal extract according to Example 6 were prepared as shown in Table 7 below. Here, the unit of the component content is% by weight.

number ingredient Formulation Example 1 Comparative Formulation Example 1 One Purified water Balance Balance 2 Carbomer 0.05 0.05 3 Hydroxyethyl chylcellulose 0.05 0.05 4 Butylene glycol 3.0 3.0 5 glycerin 2.0 2.0 6 Fig-1500 0.5 0.5 7 ethanol 4.0 4.0 8 Polysorbate 80 0.2 0.2 9 Triethanolamine 0.05 0.05 10 antiseptic a very small amount a very small amount 11 The mixed herbal extract according to Example 6 2.0 -

Among the components identified by the component numbers in Table 7, components 2 and 3 were first dispersed with stirring in component 1 (purified water), components 4 to 6 were added, components 8 to 10 were dissolved in component 7, And component 11 dissolved in component 4 were added in order and mixed.

The Comparative Formulation Example 1 for the above-mentioned Formulation Example 1 was set so that the components other than the mixed herbal extract and the other ingredients were produced and manufactured in the same manner.

≪ Formulation Example 2 and Comparative Formulation Example 2 >

Ingredient compositions of the nutritional lotion containing the mixed herbal extract according to Example 6 were prepared as shown in Table 8 below. Here, the unit of the component content is% by weight.

number ingredient Formulation Example 2 Comparative Formulation Example 2 One Cetearyl alcohol 1.0 1.0 2 Glyceryl stearate 0.7 0.7 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Maca dean nut oil 3.0 3.0 8 Butylene glycol 4.0 4.0 9 glycerin 4.0 4.0 10 Carbomer 0.15 0.15 11 Xanthan gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Balance Balance 14 Arginine 0.15 0.15 15 The mixed herbal extract according to Example 6 5.0 -

Of the components identified by the respective component numbers in Table 8, the components 1 to 7 are first melted by heating at a temperature of 70 캜, and then the components 8 to 12 are dissolved and dispersed in the component 13 and heated to 70 캜 to emulsify. Thereafter, the emulsified product was neutralized with the component 14, cooled to a temperature of 56 ° C, and then the component 15 dissolved in the separated component 8 was added and stirred, followed by cooling at room temperature.

The Comparative Formulation Example 2 for the above-mentioned Formulation Example 2 was prepared by proceeding in the same manner as the other components except for the mixed herbal extract according to Extraction Example 5,

≪ Formulation Example 3 and Comparative Formulation Example 3 >

The composition of the nutritional essence containing the mixed herbal medicine extract according to the above Extraction Example 6 was constructed as shown in Table 9 below. Here, the unit of the component content is% by weight.

number ingredient Formulation Example 3 Comparative Formulation Example 3 One Purified water Balance Balance 2 Carbomer 0.2 0.2 3 Hydroxyethyl chylcellulose 0.1 0.1 4 Butylene glycol 8.0 8.0 5 glycerin 6.0 6.0 6 Sodium hyaruronate 150 15.0 7 ethanol 4.0 4.0 8 Polyoxyethylene hardened castor oil 0.1 0.1 9 Triethanolamine 0.2 0.2 10 antiseptic a very small amount a very small amount 11 The mixed herbal extract according to Example 6 10.0 -

Of the components identified by the respective component numbers in Table 9, the components 2 and 3 were first dispersed with stirring in the component 1 (purified water), then the components 4 to 5 and 6 were added, and the components 8 to 10 were dissolved in the component 7 And component 11 dissolved in the fractionated component 4 were added in order and mixed.

The comparative formulation example 3 for the above-mentioned formulation example 3 was set to be prepared by proceeding in the same manner as the other components except for the mixed herbal medicine extract according to Extraction Example 5,

≪ Formulation Example 4 and Comparative Formulation Example 4 >

The composition of the nutritional cream containing the mixed herbal medicine extract according to the above Extraction Example 6 was prepared as shown in Table 10 below. Here, the unit of the component content is% by weight.

number ingredient Formulation Example 4 Comparative Formulation Example 4 One Cetearyl alcohol 1.5 1.5 2 Glyceryl stearate 1.0 1.0 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 Squalane 8.0 8.0 7 Apricode kernel oil 4.0 4.0 8 Dimethicone 1.0 1.0 9 Butylene glycol 5.0 5.0 10 Grill serine 4.0 4.0 11 Magnesium aluminum silicate 0.2 0.2 12 Xanthan gum 0.05 0.05 13 antiseptic a very small amount a very small amount 14 Purified water Balance Balance 15 The mixed herbal extract according to Example 6 10.0 -

On the other hand, among the components identified by the respective component numbers in Table 10, the components 1 to 8 are first melted by heating at a temperature of 70 DEG C, and then the components 9 to 13 are dissolved and dispersed in the component 14 and heated to 70 DEG C to emulsify . Thereafter, the emulsified product was cooled to a temperature of 56 ° C, and the component 15 dissolved in the separated component 9 was added and stirred, followed by cooling at room temperature.

The Comparative Formulation Example 4 of the above-mentioned Formulation Example 4 was set up so that the components other than the mixed herbal extract according to Extraction Example 5, which is the ingredient 15, were manufactured by proceeding in the same manner.

≪ Formulation Example 5 and Comparative Formulation Example 5 >

The composition of the nutritional pack containing the mixed herbal medicine extract according to the above Extraction Example 6 was prepared as shown in Table 11 below. Here, the unit of the component content is% by weight.

number ingredient Formulation Example 5 Comparative Formulation Example 5 One Purified water Balance Balance 2 Butylene glycol 3.0 3.0 3 glycerin 2.0 2.0 4 Polyvinyl alcohol 2.0 2.0 5 Polyvinylpyrrolidone 10.0 10.0 6 Cellulose sword 0.3 0.3 7 Mica 2.0 2.0 8 kaoline 2.0 2.0 9 ethanol 5.0 5.0 10 Polyoxyethylene hardened castor oil 0.3 0.3 11 antiseptic a very small amount a very small amount 12 The mixed herbal extract according to Example 6 15.0 -

Among the components identified by the respective component numbers in Table 11, Components 2 and 3 are first added to Component 1 (purified water), then Components 4 to 6 are added, and the mixture is heated to 70 캜 while stirring and dispersing. The following components 7 to 8 are added to thoroughly disperse and cool to a temperature of 56 캜. The components 10 to 11 were dissolved in the component 9 and the component 12 dissolved in the separated component 2 were added in order, and the mixture was stirred and cooled to room temperature.

The Comparative Formulation Example 5 of the above-mentioned Formulation Example 5 was set up so that the components other than the mixed herbal medicine extract according to Extraction Example 5 of ingredient 12, and the same ingredients were produced and manufactured.

<Experimental Example 3> Skin wrinkle-improving effect and skin irritation sensory test>

In order to evaluate the skin wrinkle-improving effect and the skin irritation of the cosmetic composition for improving skin wrinkles according to the present invention, a sensory test was performed using the nutritional cream prepared in Formulation Example 4 and Comparative Formulation Example 4. [

Specifically, in order to measure the effect of improving the wrinkles of the skin when each of the nutritional creams of Formulation Example 4 and Comparative Formulation Example 4 was applied to the skin, 20 women over 20 years old received the nutritional cream of Formulation Example 4 And the nutrition cream of Comparative Example 4 (control group) was continuously used for 12 weeks once a day on the right side of the face.

In the sensory evaluation test, the wrinkle improvement effect of the nutritional cream of Formulation Example 4 was relatively evaluated based on the nutritional cream of Comparative Formulation Example 4 for the skin wrinkle improvement effect item. The sensory evaluation for the skin irritation was the itching, And to evaluate the symptoms such as hot flashes and erythema. The evaluation was performed according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor (2 points), and very poor (1 point) Respectively. In Table 12, skin irritation represents a degree of no skin irritation.

number Skin irritation Wrinkle improvement effect Formulation Example 4 Comparative Formulation Example 4 Formulation Example 4 One 5 5 4 2 3 4 5 3 5 4 4 4 4 4 5 5 5 4 4 6 3 4 3 7 5 5 5 8 4 4 4 9 4 4 4 10 5 4 4 11 4 3 3 12 4 4 4 13 5 5 4 14 3 4 4 15 5 5 4 16 5 5 4 17 4 5 4 18 5 4 4 19 3 5 3 20 4 4 5 Average 4.25 4.30 4.05

As shown in Table 12, the skin irritation evaluation score of the cosmetic composition of Formulation Example 4 according to the present invention was very goodly evaluated at 4.25 points, and skin safety was excellent due to a low degree of skin irritation as in Comparative Formulation Example 4 I could confirm.

In addition, it was found that the relative skin wrinkle improving effect of the cosmetic composition of Comparative Example 4, Comparative Formulation Example 4, was 4.05 points, which is superior.

<Experimental Example 4: Safety Test of Skin>

In order to determine the occurrence of irritation or allergic reaction through the observation of the skin safety, that is, the skin reaction for the mixed herbal medicine extract according to the above-mentioned Extraction Example 6 and the Formulation Example 4, according to the Korean Food and Drug Administration standard skin safety test method, Test.

Thirty healthy male and female subjects aged 20 to 50 years old were selected as test subjects. Patch tests were performed on the inside of the upper abdomen of the subjects and after 48 hours, the epidermal patches were removed. The first reading was performed after about 60 minutes of stabilization, and the second reading was performed after 96 hours of attachment. The determination criteria are shown in Table 13 below.

reaction weight Criteria for judgment - 0 No reaction ± 0.5 Faint erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Marked erythema, papule and blister

The skin response to the sample was calculated using the following equation (4) after 48 hours and 96 hours after the attachment of the patch.

                   Σ (Weight) X (Number of Reaction)

Skin response (%) = ------------------------------- X 100

                  3 (weight) X 30 (total number of people)

Table 13 shows the skin irritation of the samples showing the positive reaction calculated based on the test results of the patch test.

sample 48 hours 96 hours Response (%) + ++ + ++ 48 hours 96 hours Average Example 6 ? ? ? ? 0.00 0.00 0.00 Formulation Example 4 ? ? ? ? 0.00 0.00 0.00

As shown in Table 14 above, each of Extraction Example 6 and Formulation Example 4 showed good results in the case of skin-sensitive skin using the non-irritating skin according to the skin response criteria according to Table 15 in the human skin test. Therefore, the mixed herbal extract according to the above extracting examples was found to be a safe ingredient without natural side effects as a natural product.

Average skin response Criteria 0.0 to 0.9 No stimulation 1.0 to 2.9 Light stimulus 3.0 ~ 4.9 Moderate irritation 5.0 or higher River stimulation

Claims (9)

delete The crude extract of crude herb medicine is extracted by solvent extraction of mixed herbal medicines of Baekbuja, Baekji, Youngryungbyeo, Hyangbuja, Baekbokryeong and Gamsohyeong, and then the crude herbal medicine crude extract is treated with carbohydrate hydrolase and pectin hydrolase Of herbal extracts for improving skin wrinkles. delete The method according to claim 2, wherein the solvent is one pure solvent selected from the group consisting of purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, a solvent consisting of ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol, A mixed herbal extract for improving skin wrinkles characterized by being a mixed solvent in which two or more of them are combined. delete The method according to claim 2, wherein the carbohydrate hydrolase is any one of Viscozyme ^® L (Glucanase, 100 FBG / g: Novozymes, Denmark) or Celluclast ^® 1.5 LFG (Cellulase, 700 EGU / g: Novozymes, Denmark) Wherein the pectin hydrolytic enzyme is Pectinex ^® 100 L (Pectinase, 5,000 FDU / mL: Novozymes, Denmark). A cosmetic composition for improving skin wrinkles containing the mixed herbal extract of any one of claims 2, 4, and 6 as an active ingredient. [Claim 7] The cosmetic composition for improving skin wrinkles according to claim 7, wherein the content of the mixed herbal extracts contained in the cosmetic composition is 0.001 to 50% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 7, wherein the cosmetic composition is at least one selected from the group consisting of cosmetics, essences, creams, lotions, packs, foundations, and makeup bases and is selected from the group consisting of liquid, cream, paste, Wherein the cosmetic composition has a property of improving skin wrinkles.