CN110840799A

CN110840799A - Whitening, moisturizing, antibacterial and mosquito-repellent body lotion and preparation method thereof

Info

Publication number: CN110840799A
Application number: CN201910990671.XA
Authority: CN
Inventors: 谢佩明; 刘婧
Original assignee: Guangzhou Yihui Biotechnology Co Ltd
Current assignee: Guangzhou Yihui Biotechnology Co Ltd
Priority date: 2019-10-18
Filing date: 2019-10-18
Publication date: 2020-02-28

Abstract

The invention relates to a whitening, moisturizing, bacteriostatic and mosquito-proof body lotion and a preparation method thereof, and SOD is added to remove free radicals and reduce melanin formation; adding glabridin to inhibit tyrosinase activity and reduce melanin formation; the nicotinamide is added to inhibit the transfer of melanin granules to keratinocytes and accelerate the skin renewal speed; the vitamin C is added to reduce melanin intermediates and melanin polymers, so that the formation of dopa and dopaquinone substances is reduced, skin is whitened in multiple ways, the effect is remarkable, and the components are safe. Adding multiple natural components of dandelion extract, stemona root extract, matrine, cnidium lactone and moschus essential oil to inhibit bacterial growth, and having the advantages of broad-spectrum antibacterial effect, long-acting effect, no drug resistance and safety; the small molecular hyaluronic acid, the mineral oil and the isopropyl myristate are added to improve the water content and the water locking degree of skin, and the citronella oil is added to effectively remove mosquitoes.

Description

Whitening, moisturizing, antibacterial and mosquito-repellent body lotion and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to whitening, moisturizing, antibacterial and mosquito-proof body lotion and a preparation method thereof.

Background

Healthy and fair skin is a focus of attention of most Asian women, Chinese women advocate the whitening effect of 'skin like snow and skin like grease', and in recent years, the demand of whitening cosmetics is increased along with the improvement of the life quality of people. There are many methods for whitening, such as:

(1) preventing melanin synthesis

Tyrosinase, dopachrome tautomerase and 5, 6-dihydroxyindole-2-carboxylic acid oxidase are the most important biological enzymes in the process of melanin formation, wherein the tyrosinase is the rate-limiting enzyme in the process, the tyrosinase mainly catalyzes the hydroxylation of monophenol to generate a dihydroxy compound and the oxidation of catechol to generate catechol, the two reactions have oxygen radicals to participate, and the activity of the tyrosinase can be damaged by removing the oxygen radicals;

(2) preventing melanin transfer

Azelaic acid, nicotinamide (VB)₃) The skin whitening agent can inhibit the transfer of melanin granules from melanocytes to keratinocytes, thereby having the skin whitening effect;

(3) accelerating melanin metabolism

The vitamin E, the vitamin C and the derivatives thereof can reduce the oxidized melanin particles into white reduced melanin particles, so that the whitening effect is achieved;

(4) oxidation resistant whitening

After being irradiated by ultraviolet rays, active oxygen is generated, so that a phospholipid membrane is damaged, skin tissues are damaged, problems of erythema, pigmentation and the like are caused, pressure is brought to the skin, and more melanin is generated. Anti-oxidation (Anti-Oxidant) is short for Anti-oxidation free radical, a complete set of Anti-oxidation system is arranged in a body, under normal physiological conditions, the body can continuously generate free radicals and continuously remove the free radicals while being subjected to oxidative metabolism, the free radicals and the free radicals are removed in a relatively dynamic balance state in the body, the generation of free radical scavengers of the body is reduced due to aging, unreasonable diet, pressure and the like, excessive free radicals can have harmful effects on the body, the excessive free radicals can attack unsaturated fatty acids in a biomembrane lipid bilayer to cause lipid peroxidation, the structure and function of a biomembrane are changed to cause cell necrosis, the functions of tissues and organs are influenced and aged, the free radicals attack protein to cause the protein to lose functions to cause pathological changes, and if the free radicals occur in collagen tissues, the skin can lose elasticity, free radicals attack DNA, which can cause cutting of DNA strands or base change, cause genetic errors to cause canceration, and reasonable dietary nutrition supplement and exogenous antioxidant nutrient intake can prevent damage of free radicals to cells, delay aging and prevent related diseases.

Superoxide dismutase (SOD) is an important component of an antioxidant enzyme system in a biological system, is widely distributed in microorganisms, plants and animals, can efficiently eliminate free radicals in the body, can cause cell damage and pigmentation due to redundant free radicals in the body, has the effects of accumulating free radicals and clearing obstacles on human body aging, and can whiten skin and delay aging by supplementing exogenous SOD.

Water is the basis of human body, the water content in human body varies according to different development stages, the water in fetus accounts for about 90%, the water in infant period accounts for 80%, the water in young and old people accounts for 70%, the water in middle-aged and old people accounts for 60% and even 50%, and the aging process of human body is also the process of water loss. The water is a special-effect and cheap cosmetic agent for protecting the skin to be clean, moist and delicate, when the moisture on the surface layer of the skin of a human body is 12-15%, the skin is smooth and elastic, and once the skin lacks the moisture, the skin can be peeled, wrinkles are grown, and the pigmentation is accelerated.

Sweat is secreted from sweat glands, namely exocrine glands and endocrine glands, contains substances such as fat, protein and the like besides water, salt and urea, and bacteria secrete protein and fat to generate ammonia and fatty acid, so that unpleasant sweat smell is generated, and the growth of the bacteria is inhibited to avoid the generation of the sweat smell.

In summer, the weather is hot, mosquitoes breed, and the itching and discomfort caused by the biting of the mosquitoes can be caused to people, and various viruses such as eastern equine encephalitis virus, Venezuelan encephalitis virus, Sindbis disease, chikungunya virus, Japanese encephalitis virus, St.Louis virus, dengue virus, yellow fever virus, West Nile virus and the like can be transmitted, so that encephalitis, hemorrhagic fever, hepatitis, fever, slight discomfort, arthritis and the like can be caused.

The patents related to the research content of the present invention are as follows:

CN106265307B discloses a tightening skin-refreshing body lotion, the active ingredients of which comprise rhubarb extract, dried ginger extract, tuckahoe extract, wild chrysanthemum flower extract, green tea extract, grape seed extract and tea polyphenol. It has effects in promoting blood circulation, improving skin elasticity and luster, compacting and shaping skin, inhibiting activity of collagenase and elastase, and preventing wrinkle. Meanwhile, the pigment has antioxidant and antibacterial effects, can prevent the generation of pigment precipitate, and has repairing effect on color spots.

CN104958247B discloses a body lotion containing nostoc sphaeroides extract, which comprises nostoc sphaeroides extract, glycerin, caprylic/capric triglyceride, hydrogenated polyisobutene, shea butter, cetostearyl alcohol, betaine, glyceryl polyether-26, polydimethylsiloxane, a complex of cetostearyl olive oleate and sorbitan olive oleate, a complex of glyceryl stearate and PEG-100 stearate, peony seed oil, tremella polysaccharide, tocopherol acetate, water, butylene glycol, centella asiatica extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea leaf extract, glycyrrhiza glabra root extract, a complex of chamomile flower extract and rosemary leaf extract, oat β -dextran, allantoin, carbomer, triethanolamine, EDTA disodium, essence, etc., and the solvent is water.

CN104622760B discloses a body care composition and a preparation method thereof. The body care composition comprises a body moistening composition and a moisturizing and pyrogenic composition which are mixed and smeared according to the weight percentage of 1: 10-10: 1 to generate heat. The moisturizing and heating composition comprises the following raw material components: anhydrous sulfate is mixed in the proportion of 1: 1-7: 1. The body moistening composition comprises the following raw material components: 0.05 to 0.2 percent of thickening agent; 0.02-0.07% of chelating agent; 8-27% of grease; 1-3.5% of emulsifier; 0.01 to 0.1 percent of antioxidant; 0.1 to 1.5 percent of conditioner; 0.5-3% of extract; the balance of water. Has the general effects of moistening and caring skin, and can generate heat when being applied, so that the body is heated and warmed, and the blood circulation is promoted.

CN106265307A discloses a tightening skin-refreshing body lotion, the active ingredients of which comprise rhubarb extract, dried ginger extract, tuckahoe extract, wild chrysanthemum flower extract, green tea extract, grape seed extract and tea polyphenol. It has effects in promoting blood circulation, improving skin elasticity and luster, compacting and shaping skin, inhibiting activity of collagenase and elastase, and preventing wrinkle. Meanwhile, the pigment has antioxidant and antibacterial effects, can prevent the generation of pigment precipitate, and has repairing effect on color spots.

CN104958247A discloses a body lotion containing nostoc sphaeroides extract, which comprises nostoc sphaeroides extract, glycerin, caprylic/capric triglyceride, hydrogenated polyisobutene, shea butter, cetostearyl alcohol, betaine, glyceryl polyether-26, polydimethylsiloxane, a complex of cetostearyl olive oleate and sorbitan olive oleate, a complex of glyceryl stearate and PEG-100 stearate, peony seed oil, tremella polysaccharide, tocopherol acetate, water, butylene glycol, centella asiatica extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea leaf extract, glycyrrhiza glabra root extract, a complex of chamomile flower extract and rosemary leaf extract, oat β -dextran, allantoin, carbomer, triethanolamine, EDTA disodium, essence, etc., and the solvent is water.

CN103976912A discloses an eighteen-symptom extracting solution and a preparation method and application thereof. The eighteen-disease extracting solution has the effects of relieving itching, relieving pain and inhibiting bacteria due to the characteristics of calming, resisting inflammation, dissipating fire, reducing swelling, astringing, promoting percutaneous absorption and inhibiting pathogenic bacteria, has the effect of preventing skin damage caused by itch from causing infection, and has the remarkable anti-allergic and itching-relieving effects when being applied to cosmetics.

CN103961291A discloses an elsholtzia extract and a preparation method and application thereof. The elsholtzia extract prepared by water extraction has the obvious effects of resisting free radicals, oxidation, radiation, bacteria and bacteria, promoting skin cell proliferation, protecting skin from ultraviolet injury and preventing skin from oxidative damage, so that the elsholtzia extract has the effects of preventing skin from oxidative damage, reducing melanin synthesis and delaying skin aging, and has the obvious effects of resisting allergy and resisting aging when being applied to cosmetics.

CN102641232B discloses rhodiola crenulata body lotion with whitening and nourishing effects, which is mainly prepared from rhodiola crenulata root extract, blueberry fruit extract, black tea extract, palmarosa oil and corresponding auxiliary materials; the rhodiola crenulata provided by the invention can moisten and moisturize, maintain the constant moisture elasticity of skin, has the functions of resisting oxidation and whitening, and is recorded as a gold plant in the compendium of materia Medica. The blueberry is rich in anthocyanin which is a pure natural anti-aging nutritional supplement. The black tea can moisten skin, promote blood circulation, activate cells, and has antibacterial and refreshing effects. The rose can restore the skin to a moisture-keeping state, regulate sebum secretion and promote cell regeneration, and the pleasant fragrance of the rose has the effects of soothing mood and promoting vibration. Rhodiola crenulata is taken as a monarch, blueberry is taken as a minister, black tea is taken as an assistant, and roselle is taken as a guide, so that free radicals can be effectively eliminated, a defense line is built for skin, and the skin is moisturized, moistened and naturally white.

Disclosure of Invention

No body lotion which has the functions of whitening, moisturizing, inhibiting bacteria and preventing mosquitoes is available in the market.

In order to solve the technical problems, the invention adopts the technical scheme that the whitening, moisturizing, antibacterial and mosquito-proof body lotion is prepared from the following components in parts by weight: 0.03-0.06% of glabridin, 2-4% of SOD, 1-4% of nicotinamide, 0.01-0.03% of micromolecular hyaluronic acid, 1-4% of vitamin C, 1-5% of mineral oil, 3-5% of isopropyl myristate, 5-8% of dandelion extract, 5-8% of radix stemonae extract, 0.05-1% of matrine, 0.05-1% of osthole, 0.05-0.2% of moschus essential oil, 0.05-0.2% of citronella essential oil, 0.05-1% of xanthan gum and the balance of deionized water.

The preparation method comprises the following steps:

the method comprises the following steps: mixing and stirring micromolecular hyaluronic acid, xanthan gum, dandelion extract, stemona root extract, matrine, osthole, SOD, nicotinamide, vitamin C and deionized water according to the formula ratio until the components are completely dissolved to form a water phase;

step two: mixing mineral oil and isopropyl myristate, and stirring for 10-20min to obtain oil phase;

step three: mixing the water phase and oil phase, stirring for 10-20min, adding moschus essential oil and herba Cymbopogonis Citrari essential oil, stirring, homogenizing in homogenizer for 30-40min to obtain emulsified mixture;

step four: adjusting the pH value of the mixture obtained in the third step to 5.5-6.5;

step five: sterilizing and canning to obtain the product.

Part of the raw material purchase information of the invention is as follows:

SOD was purchased from Wuhan remote science and technology development, Inc.;

the herba Taraxaci extract is obtained from New Tian region Biotech limited;

osthole is purchased from Shanxi Yongyuan biotechnology limited;

matrine was purchased from west ampere, east China biotechnology limited;

radix Stemonae extract is obtained from radix Angelicae Dahuricae Biotech, Inc.;

moschus essential oil was purchased from Jiangxi globulus natural perfume Co., Ltd;

citronella essential oil is purchased from Hecheng Natural perfume oil Co., Ltd, Jiangxi;

specifically, the SOD used in the invention can remove superoxide anion free radicals and hydroxyl free radicals generated by organism metabolism, and simultaneously can be used as a substrate of superoxide anions to induce enzyme so as to protect organism cells from being damaged by aerobic metabolites. The SOD as a good free radical scavenger has good effects of improving the immunity of the organism, delaying aging and the like. Supplementing exogenous SOD, increasing SOD content and activity, scavenging free radicals, and maintaining normal operation of organs and tissues.

Glabridin is a flavonoid substance, and is known as 'whitening gold' due to its strong whitening effect. The composition is gradually concerned in the beauty industry as the most expensive and safe freckle-removing whitening component in the world. The glabridin can remove a part of tyrosinase from a catalytic ring for melanin synthesis mainly by competitively inhibiting the activity of the tyrosinase, and prevent the combination of a substrate and the tyrosinase, thereby inhibiting the synthesis of the melanin.

Nicotinamide, also known as Nicotinamide (NAA), vitamin B₃Vitamin PP is widely applied to clinical treatment for preventing pellagra, glossitis, stomatitis and dermatitis. It can inhibit the formation of melanin granules, also can inhibit the melanin granules from ascending to cuticle to effectively inhibit the transfer of melanin to cuticle, and when some melanin inevitably reaches the surface skin, the nicotinamide can promote the melanocyte to drop by accelerating the renewal speed of skin cells.

Vitamin C is a water-soluble strong antioxidant, which can combine with oxygen free radicals in vivo to generate a reduction reaction, reduce ester peroxidation, reduce the generation of lipid peroxides, protect activities such as SOD and the like, prevent the oxidation process of melanin, inhibit the formation of melanin, reduce the formation of dopa and dopaquinone substances and inhibit the generation of melanin by influencing various links of melanin formation such as reduction of melanin intermediates and melanin polymers.

Moschus is plant of genus moschus of family Scrophulariaceae, and is named as thyme, herba Limoniae, herba Boschniakiae Rossicae, and herba Hierochloes Adoratae. Warm in nature and pungent in taste, has the effects of sterilizing, diminishing inflammation, dispelling wind-damp, eliminating swelling and toxin, promoting qi circulation, removing blood stasis and relieving pain, and is mainly used for treating rheumatic osteodynia, qi stagnation and abdominal pain, sore, furuncle and swelling toxin, skin eczema, pruritus and traumatic injury. The moschus volatile oil has strong fragrance, and also has antioxidant, antiinflammatory, anticancer and anticancer effects on lung cancer, skin cancer, hepatocarcinoma, etc.

The herba Taraxaci is perennial herb of Compositae, mainly contains chemical components such as flavonoid, triterpenes, sesquiterpene lactones, pigments, volatile oil and phytosterol, and has effects of clearing away heat and toxic materials, inducing diuresis for treating stranguria, relieving swelling and resolving hard mass. The dandelion extract has good antibacterial and anti-inflammatory functions, and has obvious antibacterial effects on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus subtilis, salmonella enteritidis and candida albicans. The herba Taraxaci extract contains herba Taraxaci flavone as main active ingredient.

Stemona root, radix Stemonae, slightly warm in nature, sweet and bitter in taste, is indicated for moistening lung, expelling pathogenic qi, relieving cough and killing parasites. It is used externally to treat head louse, body louse, enterobiasis and pudendum pruritus. Alkaloid contained in radix Stemonae has inhibitory effect on most pathogenic bacteria and dermatophytes.

Radix Sophorae Flavescentis is a plant of Sophora of Leguminosae, is cold in nature and bitter in taste, and is mainly used for clearing heat, killing parasite, promoting urination, eliminating dampness, relieving itching, etc. Matrine is a kind of alkaloid extracted from radix Sophorae Flavescentis, and has obvious inhibiting effect on dysentery bacillus, Escherichia coli, Bacillus proteus, type A streptococcus, type B streptococcus and Staphylococcus aureus.

Fructus Cnidii is fruit of cnidium monnieri (L.) DC of Umbelliferae, has warm nature, pungent and bitter taste, and has effects of dispelling pathogenic wind, removing dampness, killing parasite, relieving itching, warming kidney and tonifying yang. Osthole is coumarin component extracted from fructus Cnidii, and has strong inhibitory effect on bacteria and fungi.

The citronella grass is a perennial herb of the genus pilus, is called lemongrass, has warm property and pungent taste, and has the effects of dispelling wind, dredging collaterals, warming middle energizer, relieving pain, harmonizing stomach, ventilating, restoring consciousness, promoting urination, reducing blood pressure, resisting cancer, resisting malaria and the like. Citronella essential oil is a natural mosquito repellent component, primarily due to its high content of citronellal and geraniol.

The small molecular hyaluronic acid has the characteristics of rapid penetration and absorption, can rapidly synthesize the hyaluronic acid of the skin through the action of skin fiber cells, increases the content of endogenous hyaluronic acid, and makes the skin moist and smooth.

Compared with the prior art, the invention has the beneficial effects that:

the whitening, moisturizing, antibacterial and mosquito-repellent body lotion has the advantages that the SOD is added to remove free radicals and reduce the formation of melanin; adding glabridin to inhibit tyrosinase activity and reduce melanin formation; the nicotinamide is added to inhibit the transfer of melanin granules to keratinocytes and accelerate the skin renewal speed; the vitamin C is added to reduce melanin intermediates and melanin polymers, so that the formation of dopa and dopaquinone substances is reduced, skin is whitened in multiple ways, the effect is remarkable, and the components are safe. Adding multiple natural components of dandelion extract, stemona root extract, matrine, cnidium lactone and moschus essential oil to inhibit bacterial growth, and having the advantages of broad-spectrum antibacterial effect, long-acting effect, no drug resistance and safety; the small molecular hyaluronic acid, the mineral oil and the isopropyl myristate are added to improve the water content and the water locking degree of skin, and the citronella oil is added to effectively remove mosquitoes.

Drawings

FIG. 1 shows superoxide anion removal by SOD and vitamin C;

FIG. 2 shows the hydroxyl radical scavenging rates of SOD and vitamin C;

FIG. 3 example one to example five pairs of superoxide anion, hydroxyl radical scavenging rates;

FIG. 4 results of skin brightness experiments;

FIG. 5 results of the melanin reduction ratio;

fig. 6 stratum corneum moisture change.

Detailed Description

The present invention is further described below by way of specific examples, but the present invention is not limited thereto.

Example one

The formula is as follows: glabridin 0.06%, SOD 4%, nicotinamide 4%, small molecular hyaluronic acid 0.01%, vitamin C4%, mineral oil 1%, isopropyl myristate 3%, dandelion extract 5%, radix stemonae extract 5%, matrine 0.05%, osthole 0.05%, moschus oil 0.05%, citronella essential oil 0.05%, xanthan gum 1%, and the balance of deionized water;

the preparation process comprises the following steps:

step five: sterilizing and canning to obtain the product.

Example two

The formula is as follows: glabridin 0.03%, SOD 3%, nicotinamide 3%, small molecular hyaluronic acid 0.03%, vitamin C3%, mineral oil 5%, isopropyl myristate 5%, dandelion extract 6%, radix stemonae extract 6%, matrine 0.06%, osthole 0.06%, moschus oil 0.06%, citronella essential oil 0.06%, xanthan gum 0.08%, and the balance of deionized water;

the preparation process is the same as that of the first embodiment.

EXAMPLE III

The formula is as follows: glabridin 0.04%, SOD 3%, nicotinamide 2%, small molecular hyaluronic acid 0.02%, vitamin C2%, mineral oil 4%, isopropyl myristate 4%, dandelion extract 8%, radix stemonae extract 8%, matrine 1%, osthole 1%, moschus essential oil 0.2%, citronella essential oil 0.1%, xanthan gum 0.06%, and the balance of deionized water;

the preparation process is the same as that of the first embodiment.

Example four

The formula is as follows: glabridin 0.05%, SOD 2%, nicotinamide 1%, small molecular hyaluronic acid 0.01%, vitamin C1%, mineral oil 2%, isopropyl myristate 3%, dandelion extract 7%, radix stemonae extract 7%, matrine 0.07%, osthole 0.07%, moschus essential oil 0.1%, citronella essential oil 0.2%, xanthan gum 0.07%, and the balance of deionized water;

the preparation process is the same as that of the first embodiment.

EXAMPLE five

The formula is as follows: glabridin 0.06%, SOD 4%, nicotinamide 4%, small molecular hyaluronic acid 0.03%, vitamin C4%, mineral oil 5%, isopropyl myristate 5%, dandelion extract 8%, radix stemonae extract 8%, matrine 1%, osthole 1%, moschus essential oil 0.2%, citronella essential oil 0.2%, xanthan gum 0.05%, and the balance of deionized water;

the preparation process is the same as that of the first embodiment.

Experiment one: tyrosine activity inhibition assay

Experimental reagents and instruments: tyrosine, L-ascorbic acid-glucoside, glabridin, BSA223S-G analytical balance (Shanghai Jingtian electronic instruments Co.), Intelligent digital display constant temperature water bath (Jiangsu Jinyi instruments science and technology Co., Ltd.), DR6000 ultraviolet-visible spectrophotometer (American Hash Co., Ltd.)

The experimental method comprises the following steps: using 0.01mol/l phosphate buffer pH6.8 and 1.5g/l levodopa as a substrate, 40. mu.l of the substrate was applied to a 96-well plate, and 80. mu.l of the phosphate buffer and 40. mu.l of the sample were added thereto. Then adding 40 mul of tyrosinase with the concentration of 100U/ml, mixing uniformly, reacting in a water bath at the temperature of 30 ℃ for 30min, and measuring the absorbance at 490 nm. The blank used 40. mu.l of the above phosphate buffer. The tyrosinase activity inhibition rate was calculated as follows.

Inhibition rate = [1- (T)₁-T₂)/(C₁-C₂)]×100%

In the formula C₁Absorbance when no enzyme was added to the sample, C₂Absorbance without enzyme and without sample, T₁Is the absorbance, T, at the time of addition of enzyme to the sample₂Absorbance without enzyme plus sample.

The experimental results are as follows:

experiment two: in vitro antioxidant Activity test

The experiments were divided into 7 groups: SOD, vitamin C, example one to example five.

Experimental apparatus and reagents: BSA223S-G analytical balance (Shanghai Jingtian electronic instruments Co.), DR6000 ultraviolet-visible spectrophotometer (American Hash Co.), SIGMA3-18K refrigerated centrifuge (Beijing Boxixing instruments), intelligent digital display thermostat water bath (Jiangsu Jinyi instruments science and technology Co., Ltd.), superoxide anion kit and hydroxyl radical kit were all purchased from Nanjing to build the bioengineering institute.

The experimental method comprises the following steps: the assay was performed with reference to kit instructions.

The experimental results are shown in fig. 1, 2 and 3.

According to the experimental results, the SOD shows high superoxide anion free radical clearance and hydroxyl free radical clearance at lower concentration. The antioxidant capacity SOD is larger than vitamin C, and the antioxidant capacity is improved through the synergistic effect of the SOD and the vitamin C. The effect of the fifth embodiment is the best.

Experiment three: whitening and freckle removing experiment

An experimental instrument: minolta Chromameter CM-700d (Meinenda, Japan), Mexameter MX18 skin melanin and heme tester (Courage + Khazak, Germany).

The experimental method comprises the following steps:

1. volunteer subjects were recruited 100, aged 18-66 years.

2. After bathing every night, the body is smeared on the whole body by using the body cream with the five formulas of the examples, and 1 treatment course is carried out for 20 days.

3. The skin condition of the subject was measured with the instrument before, on day 20, day 40, day 60, and day 80, respectively. Skin lightness L values were measured using a Minolta Chromameter (CM-700d), 3 times per replicate, averaged, and the L values of all volunteers were re-averaged. The skin melanin values were measured using a Mexameter MX18, 3 replicates each time, averaged, the melanin reduction ratios calculated, and the melanin reduction ratios of all volunteers were then averaged.

4. The results of the experiment are shown in FIGS. 3 and 4.

The experimental results show that the product can brighten the skin color and reduce the whitening effect of melanin.

Experiment four: skin moisturizing test

The experimental population is as follows: 100 healthy female volunteers, age 18-65 years.

Sample preparation: example one to implementation four

An experimental instrument: skin cuticle moisture tester (Courage & Kazaka, germany),

the test method comprises the following steps:

the test part does not use any cosmetics 2-3 days before the test base value, and does not contact water for 1-3 hours. Before the test, the testee cleans the front wall of the front wall by using soap, and after the front wall is cleaned, five marks are made on the testee, namely a blank control area, an area from the first embodiment to the fifth embodiment, and an area of the experimental area is 3 multiplied by 3cm²Each region being spaced 2cm apart. Sit still in a room with constant temperature and humidity (temperature 21 +/-0.5 ℃ and humidity 50 +/-2%) for 30min before formal test. Five areas of stratum corneum moisture content were tested 20, 40, 60, 80 days before using the samples. The average value of each measured data was calculated.

The experimental results are shown in FIG. 5.

Experiment five: experiment for inhibiting bacteria

Experimental reagents and instruments: an autoclave YM30B (Shanghai Sanshen medical instruments Co., Ltd.), a constant temperature incubator (Guangdong Tai Hongjun scientific instruments Co., Ltd.), and an ultra-clean bench (Shanghai Lujing medical instruments Co., Ltd.). Bacillus subtilis [ CMCC (B)63501], Staphylococcus aureus [ CMCC (B)26003], Escherichia coli [ CMCC (B)44102], Candida albicans [ CM-CC (F)98001], nutrient agar medium, and double lactose-bile medium.

The experimental method comprises the following steps:

1. adding nutritional agar slant culture of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Candida albicans cultured at 36 deg.C for 24 hr into 0.9% sterile sodium chloride solution with turbidity equivalent to that of standard turbidimetric solution as stock solution; bacterial suspension containing 50-100CFU bacteria per 1ml is prepared by 0.9% sterile sodium chloride solution.

2. Taking 1ml of the experimental bacteria (staphylococcus aureus, bacillus subtilis and candida albicans) of the five examples and 50-100CFU, respectively injecting the experimental bacteria into plates, immediately pouring nutrient agar plates which are melted and cooled to 45 ℃, and preparing 2 plates in parallel for each strain. Taking 7 double lactose-bile salt culture medium tubes, respectively adding 10ml of Escherichia coli, 1ml of Escherichia coli +9ml of the first to fifth emulsions and 10ml of diluent, and culturing at 44 ℃ for 24h in the culture tubes. And (5) observing the acid and gas production condition in the fermentation tube, and scratching an agar plate for confirmation experiment.

3. And (4) judging a result: the recovery rate of staphylococcus aureus, bacillus subtilis and candida albicans is more than 70 percent, and the sample has no bacteriostatic action; the recovery rate is less than 70%, and the sample has the bacteriostatic action. Positive Escherichia coli is detected, and the sample has no bacteriostatic action and is indicated by a plus sign; if the positive bacteria are not detected in the test sample added with the bacteria liquid, the sample has the bacteriostatic action and is represented by "-".

The experimental results are as follows:

result of bacteriostasis

Experiment six mosquito repelling experiment

Experimental materials: aedes albopictus was purchased from military medical research institute of the military region of beijing.

The experimental method comprises the following steps:

1. selecting qualified subjects

About 500 mosquitoes (female mosquitoes) are put into a mosquito cage of 60X 45cm, and the hands of the volunteers permeate into the mosquito cage for 2min, so that more than 30 mosquitoes which come and stop are qualified subjects and can be used for experiments.

2. The back of each hand of 50 qualified subjects exposed 7X 7cm of skin, with the remainder covered closely. Wherein 1 hand is used as a blank control, the body lotion of the first to fifth embodiments is uniformly smeared on the exposed skin of the back of the hand according to 1.5mg/cm by the other 1 hand, the hand is inserted into a mosquito cage for 2min after 2h, whether the mosquito sucks blood before is observed, the test is carried out for 1 time every hour later, the failure is judged as long as 1 mosquito sucks blood before, the average effective protection time is recorded, and the effective protection rates of 4, 6, 8, 10 and 12h are observed and calculated.

Effective protection rate = (average number of bites in control group-average number of bites in experimental group)/average number of bites in control group

The experimental results are as follows:

Claims

1. the whitening, moisturizing, antibacterial and mosquito-repellent body lotion is characterized by comprising the following components in parts by weight: 0.03-0.06% of glabridin, 2-4% of SOD, 1-4% of nicotinamide, 0.01-0.03% of micromolecular hyaluronic acid, 1-4% of vitamin C, 1-5% of mineral oil, 3-5% of isopropyl myristate, 5-8% of dandelion extract, 5-8% of radix stemonae extract, 0.05-1% of matrine, 0.05-1% of osthole, 0.05-0.2% of moschus essential oil, 0.05-0.2% of citronella essential oil, 0.05-1% of xanthan gum and the balance of deionized water.

2. The whitening, moisturizing, bacteriostatic and mosquito-repellent body lotion according to claim 1, wherein SOD is extracted from plants and has enzyme activity of 100000U/g.

3. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion as claimed in claim 1, wherein the molecular weight of the small molecular hyaluronic acid is 6000-.

4. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the content of dandelion flavone in the dandelion extract is not less than 10%.

5. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the purity of osthole is more than or equal to 98%.

6. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the musk content in the moschus essential oil is not less than 60%.

7. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the citronellal is 35-40% and the geraniol is 15-20% of the citronella essential oil.

8. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the purity of the matrine is not less than 98%.

9. The whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to claim 1, wherein the extraction ratio of the stemona root extract is 30: 1.

10. The preparation method of the whitening, moisturizing, antibacterial and mosquito-repellent body lotion according to any one of claims 1 to 9, wherein the preparation process comprises the following steps:

step five: sterilizing and canning to obtain the product.