KR101810231B1 - Cosmetic composition for improving acne containing propolis spicule powder - Google Patents

Abstract

Complex extract consisting of propolis extract, royal jelly extract, golden extract, and red ginseng extract; And a fine needle powder as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition containing a propolis micropowder powder,

The present invention relates to a cosmetic composition for improving acne containing a natural extract and fine needle powder.

The skin is an organ that protects the living body from the external environment and prevents the loss of biological components such as water and electrolytes in the human body while preventing harmful substances from entering the human body from the outside. The skin is largely divided into a skin layer, a dermal layer and a subcutaneous fat layer. The skin layer is composed of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they are required to have strong resistance against physical, chemical, or material permeation, At the same time, it is necessary to maintain proper water content to maintain flexibility.

Natural Moisturizing Factor (NMF), which is present in the keratin layer, has hydrophilic water retention capacity and plays an important role in skin moisturization. The normal keratinocyte layer is smooth and soft when the water content is maintained at about 10 to 30%, and the body protection functions normally. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes coarse, the protective function of the body is lost, and the skin aging phenomenon occurs.

In the case of dry skin, the keratinocyte aggregation is weakened and scaling phenomenon appears as peeled off from the surface of the skin. Such dryness of the skin occurs when the moisture content of the keratinocyte is reduced compared to the normal skin. In addition, even a healthy skin may cause a water shortage phenomenon due to harsh external factors such as wind, cold weather, sunlight, cleansing, shaving, etc., so that the moisture content of the keratinocyte layer is desirably maintained appropriately.

For this reason, moisturizing agents such as sebum-like components, NMF components or polyols are added to cosmetics.

Glycerin and solitol having three or more hydroxyl groups (OH groups) as the water-soluble polyol have excellent moisture resistance, but are insufficient in usability due to stickiness, and have a hydroxyl group (OH group) having two hydroxyl groups such as propylene glycol and 1,3-butylene glycol Can cause side effects to the skin. In addition, other natural moisturizing factors, such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic and may deteriorate emulsion stability of the cosmetic. Amino acids, collagen, and elastin also have moisturizing ability, but they have limited moisturizing ability.

On the other hand, acne is an inflammatory disease that occurs in the hair follicles of the skin. 70-80% of the patients are concentrated in the age group of 11-25 years. In the past, they were considered to be specific diseases for adolescent adolescents. However, It is one of the diseases.

Acne can develop symptoms due to an increase in sebum secretion, hyperkeratosis-induced follicular stenosis, proliferation of acne bacterium, inflammatory reaction, etc., and various mechanisms may be combined and adversely affected . Acne can be treated through excessive suppression of sebum secretion and relief of inflammation. Therefore, the occurrence of acne can be effectively suppressed by eliminating a complex cause that causes acne.

In general, medicines in the form of oral or topical agents may be used to remove the major cause of acne and treat acne.

Specifically, the present invention relates to a method for inhibiting sebum production using antihistamines, an antimicrobial agent such as resorcinol, sulfur, salicylic acid, benzoyl peroxide, retinoic acid, and isotretinoin for antiinflammatory action of steroid hormones and nonsteroidal antiinflammatory agents, A method of inhibiting the activity of propionibacterium acnes by an antibiotic substance such as a keratolytic agent, tetracycline, erythromycin, and macrocycline.

However, despite the fact that the acne treatment method has shown some success in treating acne, it shows some problems in terms of effectiveness, side effects, and usability.

Specifically, hormones induce hormone abuse side effects such as inhibition of epidermal growth. Keratolytic agents such as retinoic acid and benzoyl peroxide can cause contact dermatitis due to skin irritation. Antibiotics such as tetracycline may cause resistant bacteria And may be a factor of photosensitizing action.

In the cosmetics industry, many products using natural products have been developed to reduce skin irritation caused by various chemical substances. In addition to low adverse effects on the skin, natural materials have recently increased their value as a raw material for cosmetics, as consumers' responses to cosmetics using natural materials have increased. However, the cosmetic product containing the natural product extract obtained by a conventional method is not practically effective because antibacterial and anti-inflammatory functions are not effectively achieved and the activity of the extract is not maintained and controlled continuously.

Therefore, in order to treat and improve acne, it is necessary to develop a natural-derived cosmetic product which is not only safe to human body but also has excellent skin-improving effect, and has recently become a multi-functional ) Interest in the development of cosmetics is growing.

Disclosure of the Invention The present invention has been conceived to solve the above-mentioned problems, and an object of the present invention is to provide a cosmetic composition for improving acne, which comprises a natural material as an active ingredient and has excellent biocompatibility, will be.

According to one aspect of the present invention, there is provided a combination extract comprising a propolis extract, a royal jelly extract, a golden extract, and a red ginseng extract; And a fine needle powder as an active ingredient.

In one embodiment, the micropowder powder may be obtained from a sponge animal.

In one embodiment, the sponge animal may be a Spongilla lacustris .

In one embodiment, the average particle length of the powder may be from 60 to 500 탆.

In one embodiment, the combined extract comprises 20 to 50 parts by weight of the propolis extract, 10 to 25 parts by weight of the royal jelly extract, 10 to 20 parts by weight of the gold extract, and 10 to 20 parts by weight of the red ginseng extract .

In one embodiment, the complex extract may be contained in an amount of 1.0 to 30.0 wt% based on the total weight of the composition, and the micropowder powder may be contained in an amount of 0.1 to 5.0 wt%.

In one embodiment, the propolis extract may be obtained by an ultrasonic extraction method.

In one embodiment, the composition may be for acne improvement or skin cleansing.

In one embodiment, it may be formulated into one or more selected from the group consisting of softening lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder .

According to the present invention, since the cosmetic composition contains a large amount of active ingredient contained in propolis, it has excellent anti-inflammatory and antibacterial effects

In addition, the cosmetic composition can maximize the effect of improving the skin due to the synergistic action of the active ingredient contained in the complex extract, and the skin penetration effect can be remarkably improved by the fine needle powder.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to one aspect of the present invention, there is provided a combination extract comprising a propolis extract, a royal jelly extract, a golden extract, and a red ginseng extract; And a fine needle powder as an active ingredient.

Since the cosmetic composition contains the above-mentioned complex extract as an active ingredient, it has excellent skin moisturizing effect, antibacterial and anti-inflammatory activity, and has excellent acne improvement effect. In particular, the cosmetic composition of the present invention can remarkably improve the skin-improving effect as compared with a cosmetic composition containing the single extract individually because of the synergistic effect of each component contained in the complex extract.

In addition, since the cosmetic composition contains fine needle powder, penetration of the active ingredient into the skin can be significantly increased.

Spicule is a composite material composed of calcium and silicate in the form of various fine needles taken from sponges living in fresh water in Europe and Russia, and can be generally obtained in the form of powder.

The fine needle-like structure penetrates the skin layer as a fine needle-like structure and forms a micro channel, so that the penetration effect of the applied cosmetic ingredient can be remarkably increased. In addition, the fine needle can promote skin regeneration of collagen which acts on an artificial wound healing, and increase the skin tissue temperature to increase bioactivity.

The fine needle is a harmless substance to the human body and is not particularly limited as long as it is a powdery material capable of stimulating the skin to form fine pores and dissolved or discharged in the body.

In one embodiment, the micropowder powder can be obtained from a spongy animal, wherein the spongy animal is a Spongilla lacustris L. ), Spongilla fragile Leidy , and Ephydatia fluviatilis species.

The sponge animal is a primitive marine creature with no muscle, nerves or organs, and more than 5,000 species are present. The skeleton is a tissue that functions as a needle-like skeleton existing in the body of invertebrate animals, and mainly contains silicic acid and calcium carbonate. The sponge animal may comprise uniaxial, triaxial, tetragonal or multiaxial bone fragments containing calcium carbonate or silicic acid as a major component.

The sponge is sponge of various colors such as orange or cyan, and it lives mainly in the sea, but there is also a freshwater sponge.

The sponge animal is an organism that is in the initial stage of tissue in a state where cells are aggregated, and the body is composed of sponge fibers, spicules forming body wall, and cells bound to bone fragments. The main component of the bone fragments is calcite, silicic acid, silica or the like, and the size is about 100 탆 or so and can be observed by a microscope.

The bone fragments can be used as an effective means of skin regeneration to correct visual facial defects such as hyperpigmentation of various etiologies, fine wrinkles, sun damage, superficial scars, cotton wool, and enlarged pores. It is also being used as a natural alternative approach to microdermabrasion and skin erosion, chemical peeling, CO ₂ laser ablation, and erbium laser ablation, which are physical procedures to improve skin conditions.

When the bone fragments come into contact with and rub against the skin layer, they are physically separated to reduce keratinocyte aggregation, and the keratin layer, sebum plug, and keratinocytes can be desorbed to promote regeneration of the skin.

The average particle length of the fine needle-like powder may be 60 to 500 탆. If the average particle length is less than 60 탆, it can not effectively penetrate into the skin to form pores. If it is 500 탆, the dispersibility may be lowered or excessive stimulation to the skin may occur.

On the other hand, the " extract " refers to a solvent in which an effective ingredient contained in an extraction material has been transferred by bringing a solvent and an extraction material into contact under specific conditions. If the material is obtained by extracting a component contained in a raw material from a natural product, Regardless of the kind of the component. For example, it may include a component obtained by extracting a component dissolved in a solvent from a natural product using water or an organic solvent, a specific component of a natural product such as a product obtained by extracting only a specific component such as oil, and the like.

"Propolis" is a substance made by mixing bees with their own needles and enzymes in a resin-like material extracted from various plants for their survival and propagation. It contains a large amount of organic matter and minerals, and contains minerals, Vitamins, amino acids, fats, organic acids, and flavonoids play important roles in cell metabolism. The propolis has been reported to have a therapeutic effect on skin diseases, hair loss suppression effect, cosmetic effect and bacterial dental disease caused by fungi such as anticancer, burning ability, eczema, atopic dermatitis and athlete's foot, Stimulating activity, treatment of scurvy by the action of vitamin p, hypotensive effect of hypertensive patients, balance of biohormones, and so on.

The "royal jelly" is a secretion of bees used for nutrition of bee larvae. It is secreted from the pharyngeal gland at the head of young worker bees. Protein and saccharides are the main constituents, and vitamin A, B1, B2 And it contains water, lipids, saccharides, ash, enzymes, hormones, steroids and the like. Royal jelly is known to have the effect of keeping the moisture of skin surface properly when applied to the skin, improving the elasticity of the skin by increasing the metabolism of the skin, and preventing skin troubles through anti-inflammatory action.

The " golden ( Scutellaria baicalensis "refers to a medicinal product made from the roots of Scutellaria baicalensis GEORGE, a perennial herbaceous plant belonging to the family Lamiaceae. The gold generally grows in the grasses of the mountain area and grows in several layers, with hairs, hairy branches, and branches. The roots are conical and yellow in color. The roots in one room are used as fever, diuretic, branch, ejaculatory, and anti-inflammatory. On the other hand, it is known that baicalin, a type of flavonoid contained in gold, has antioxidant, anti-cancer and anti-inflammatory effects.

The above-mentioned "red ginseng" means that the perennial ginseng ( Panax ginseng C) belonging to the ginseng belonging to the genus Ginseng is cooked by steam or other means, and the origin, type and form of ginseng are not limited. Red ginseng is characterized not only by difference in appearance which shows pale yellowish brown color or light reddish brown color but also in that moisture is evaporated in the drying process and the content of physiologically active substance per unit is very high. The anti-cancer component (ginsenoside), the component inhibiting cancer cell toxin activity ), A platelet aggregation inhibiting component (maltol), an obesity inhibiting component, an aging inhibiting component, and a heavy metal detoxifying component.

The complex extract may comprise 20 to 50 parts by weight of the propolis extract, 10 to 25 parts by weight of the royal jelly extract, 10 to 20 parts by weight of the gold extract, and 10 to 20 parts by weight of the red ginseng extract.

When the variation in the mixing ratio of the complex extract is large, the skin improvement effect derived from the active ingredient of each extract may not be suitably implemented, so that the mixing ratio can be appropriately controlled in consideration of the working environment and the quality of the final product . The inventors intend to derive the optimal mixing ratio of the raw materials on the basis of the results of thousands of experiments, and the skin improving effect of the mixed extract can be maximized.

In one embodiment, the complex extract may be contained in an amount of 1.0 to 30.0 wt% based on the total weight of the composition, and the micropowder powder may be contained in an amount of 0.1 to 5.0 wt%.

If the content of the complex extract is less than 1.0% by weight, it may be difficult to obtain an expected effect. If the content of the complex extract is more than 30.0% by weight, a remarkable effect may not be realized compared with the addition amount, The inherent quality of the product may be compromised.

If the content of the fine needle-like powder is less than 0.1% by weight, the effect of improving the penetration of the skin is not sufficiently realized. If the content is more than 5.0% by weight, side effects or skin troubles may be caused by skin irritation.

On the other hand, the kind of the solvent used in the extraction step is not particularly limited, and various solvents may be used as needed. In addition, the extraction can be carried out using a conventional method in the art such as cold-rolling, warming, heating, and the like using the solvent.

The extract may be extracted with water, at least one solvent selected from the group consisting of lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, and the alcohol has a concentration of 60 to 90% (v / v) ethanol.

That is, the extraction ratio may be different depending on the polarity of the solvent, and the ethanol is excellent in the selectivity in the extraction of the physiologically active substance of the natural raw material. Therefore, Can be implemented.

Particularly, the water and ethanol have different polarities, and the effective components extracted according to the respective polarities may be different from each other, so that the concentration of the ethanol can be appropriately controlled so that an optimum skin improving effect can be realized. However, if the concentration of ethanol is more than 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improvement effect may not be extracted sufficiently. Therefore, Can be adjusted appropriately.

Meanwhile, the propolis extract can be extracted by an ultrasonic extraction method. Since the ultrasonic extraction method has a high extraction yield and excellent productivity as compared with conventional hot water extraction, room temperature extraction and warming extraction, effective components contained in propolis can be effectively extracted.

The ultrasonic extraction means that the extraction raw material is put in one or more extraction solvents selected from water, butylen glycol, propylene glycol, ethanol, and the like, and then extracted with an ultrasonic extraction device for about 1 to 12 hours. The time of the ultrasonic extraction may be different according to the amount of the sample to be extracted, and the extraction product may be filtered or purified.

According to one embodiment, the ultrasonic extraction can be firstly extracted at pH 10 to 12, and then secondarily extracted at pH 5 to 7.

Specifically, the ultrasonic extraction comprises: pulverizing the propolis raw material and suspending the propolis raw material in an alcohol aqueous solution; controlling the pH to 10 to 12; A first ultrasonic extraction step; controlling the pH to 5 to 7; And a second ultrasound extraction step.

The propolis can be efficiently extracted with the beeswax at a pH of 10 to 12, and the effective component can be efficiently extracted. Since the water-soluble propolis is dissolved in the aqueous ethanol solution under the condition of pH 5 to 7, it is preferable to adjust the pH appropriately at each step.

At this time, the frequency of the ultrasonic waves can be appropriately adjusted according to the amount and condition of the propolis raw material, and may be preferably 10 to 100 kHz.

In one embodiment, the composition may be for acne improvement or skin cleansing. The cosmetic composition may effectively penetrate and act on the skin due to the fine needle as a result of which the skin improving effect can be remarkably improved.

According to one embodiment, the composition is formulated into one or more selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, .

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

In addition, the cosmetic composition may be a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, Any other ingredient used may also additionally contain adjuvants commonly used in the cosmetics or dermatological sciences.

However, it is preferable that the adjuvant and the mixing ratio thereof are appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  1 - Preparation of extract

Royal jelly extract, golden extract, and red ginseng extract were prepared using ethanol as a solvent. Each of the extracts was prepared by refluxing the respective ingredients twice with 2 liters of 80% ethanol for 2 hours, and was obtained as a solid by concentration under reduced pressure and lyophilization.

The combined extracts were prepared by mixing 50 g of propolis extract, 20 g of royal jelly extract, 20 g of golden extract, and 20 g of red ginseng extract.

The propolis extract was prepared by a two-stage ultrasonic extraction method. The propolis raw material was pulverized by a milling machine and suspended in an 80% aqueous alcohol solution to prepare a suspension. Subsequently, alkali was added to adjust the pH to 11, and then 20 캜 was maintained. Primary ultrasonic extraction was performed by applying 70 kHz ultrasonic wave first. Next, acid was added to the first extract to adjust the pH to 6. Then, second ultrasonic extraction was performed by applying 70 kHz ultrasonic waves to the second extract. The extract was subjected to filtration and then concentrated under reduced pressure and lyophilized to give a solid ≪ / RTI >

Manufacturing example  2 - Fine needle  Powder manufacturing

Dried sponges ( Spongilla 800 g of a 0.5 N sodium hydroxide solution was added to 200 g of the natural sponge obtained in lacustris and mixed and pulverized by an ultrasonic process. After stirring, impurities were removed by centrifugation and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A small amount of sodium hydroxide was removed by chemical reaction with 800 ml of a 2% -L-ascorbic acid solution, and then the charged L-ascorbic acid solution was removed, followed by drying to obtain fine needle powder from which purified water was removed.

Manufacturing example  3 - Preparation of sample

The extracts obtained in Preparation Examples 1 and 2 and the fine needle powder were mixed to prepare a cosmetic sample as shown in Table 1 below. The results of this study are as follows: 1.

In Comparative Examples 1 to 5, the complex extract, propolis extract, royal jelly extract, golden extract, and red ginseng extract were respectively introduced.

[Content (% by weight)] division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Fine needle powder 3.0 - - - - Complex extract 10.0 10.0 Propolis extract - - 10.0 - - - Royal jelly extract - - - 10.0 - - Golden extract - - - - 10.0 - Red ginseng extract - - - - - 10.0 1,3-butylene glycol 6.0 6.0 6.0 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 Oleyl alcohol 0.1 0.1 0.1 0.1 0.1 0.1 Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 ethanol 15.0 15.0 15.0 15.0 15.0 15.0 Benzophenone-9 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance

Experimental Example  1: Assessment of cytotoxicity

The toxicity of the samples obtained in Examples and Comparative Examples 1 to 5 to cells was measured. The samples obtained in the above-mentioned Examples and Comparative Examples 1 to 5 were suspended in purified water and prepared by concentration (100, 50, 25 μg / mL), and cell viability was measured by the following method.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

HDF (Human Dermal Fibroblast) cells were seeded in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and stabilized at 37 ° C and 5% CO ₂ for 24 hours. The samples obtained in Examples and Comparative Examples 1 to 5 were treated for each concentration and cultured for 24 hours. After the medium was removed, MTT was treated at a concentration of 5 mg / mL and cultured for 2 hours. 100 μL of DMSO (dimethyl sulfoxide) was added, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of each sample. The above results suggest that the combined extract is harmless to the human body and is excellent in safety.

Experimental Example  2: Skin Moisture power  Evaluation of improvement effect

The sample (nutritional cream) shown in Table 1 was applied twice daily for 1 month to the face and the first half of 40 persons of 20 to 30 years old adults with acne, The same amount of purified water was used.

The skin conductivity was measured using a moisture meter (Corneometer, CM820 courage Khazaka electronic GmbH, Germany) at a constant temperature and humidity condition (temperature 24 ° C., humidity 40%) before the start of application, The conductivity increase rate (%) was measured.

[Moisturising activity (%)] division 2 4 6 8 10 12 14 16 18 20 Example 43.63 39.23 31.74 25.85 18.56 13.84 13.17 12.53 11.68 10.81 Comparative Example 1 35.63 31.21 23.13 17.15 11.25 8.38 8.17 8.05 7.68 6.81 Comparative Example 2 19.22 9.86 8.15 6.98 5.24 4.66 3.81 2.59 2.07 1.59 Comparative Example 3 23.82 18.89 14.21 7.66 5.81 4.96 4.81 4.01 3.22 2.86 Comparative Example 4 20.21 13.77 11.09 6.67 5.88 4.59 4.05 2.92 2.38 1.03 Comparative Example 5 21.08 15.11 8.99 7.05 6.51 6.01 4.08 3.64 3.02 2.55 Control group 18.22 10.31 7.52 5.17 4.81 4.76 2.27 1.55 1.38 0.76

As a result of the measurement, the sample of Example 1 was found to have an excellent skin conductivity increase rate as compared with Comparative Examples 1 to 5.

In general, the skin conductivity is proportional to the skin moisture content, so that the sample containing the fine needle powder and the complex extract effectively prevented the desorption of moisture in the skin and was excellent in skin moisturizing power.

Experimental Example  3: Evaluation of acne improvement effect

50 of the women aged 19 to 28 with acne were applied to the face and the first half of the formulated example and the comparative examples 1 to 5 twice a day for one month. The results are shown in Table 3 below.

As a result of the evaluation, it was confirmed that the sample (Example) of Example 1 was remarkably excellent in the effect of improving acne as compared with the samples of Comparative Examples 1 to 5.

division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 1 week ++ + ± ± ± ± 2 weeks ++ + + ± ± ± 3 weeks ++ + + + + + 4 weeks +++ ++ ++ + + +

<Evaluation Criteria>

+++: very good improvement effect / ++: considerable improvement effect / +: slight improvement effect / ±: no improvement effect but not deterioration / -: not improvement effect but rather weakness

Experimental Example  4: Improvement of atopic dermatitis

100 patients with acute exacerbated atopic dermatitis were divided into groups and samples of the Examples and Comparative Examples 1 to 5 were used. A questionnaire survey was conducted to evaluate the improvement of skin dryness after use.

At the time of application, the cream was directly applied to the skin as using a cosmetic cream, and the cream was applied to a region where symptoms of atopic dermatitis appeared.

One identical examiner assessed the degree of improvement of skin lesion and SCORAD (scoring atopic dermatitis), and the patient scored a visual analogue scale (VAS, 0 ~ 10 cm) to assess the severity of itching Respectively.

The evaluation was made within one hour after application of the sample, and the results are shown in Table 4 below.

division SCORAD score VAS score Before application After one application After 2 applications Before application After one application After 2 applications Example 52.29 39.3 34.3 9 5 3 Comparative Example 1 53.21 45.2 42.7 9 7 6 Comparative Example 2 53.13 46.3 43.1 9 8 8 Comparative Example 3 53.17 43.6 46.1 9 8 7 Comparative Example 4 53.19 44.3 40.2 9 7 7 Comparative Example 5 53.20 44.9 41.1 9 7 6

If the SCORAD score is> 40, atopic symptoms are considered serious. When the sample of Example 1 was used, the SCORAD score decreased suddenly from 52.29 to 34.3, and the atopic state remarkably improved.

A VAS score of 10 means the severity of pain. When the sample of Example 1 was used, the VAS score was significantly reduced from 9 to 3, suggesting that pain due to atopic dermatitis was significantly reduced.

The sample (Example) containing the fine needle powder and the complex extract effectively penetrates the complex extract into the skin due to the fine needle when applied to the skin, so that the symptoms of atopic dermatitis accompanying the inflammation reaction and itching can be significantly reduced.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

Complex extract consisting of propolis extract, royal jelly extract, golden extract, and red ginseng extract; And a fine needle powder as an active ingredient.
The method according to claim 1,
The fine needle powder is obtained from a sponge animal.
3. The method of claim 2,
The sponge animal is a Spongilla &lt; RTI ID = 0.0 &gt; lacustris .
The method according to claim 1,
Wherein the powder has an average particle length of 60 to 500 占 퐉.
The method according to claim 1,
Wherein the complex extract comprises 20 to 50 parts by weight of the propolis extract, 10 to 25 parts by weight of the royal jelly extract, 10 to 20 parts by weight of the gold extract, and 10 to 20 parts by weight of the red ginseng extract.
The method according to claim 1,
Wherein the composite extract is contained in an amount of 1.0 to 30.0% by weight based on the total weight of the composition, and the fine needle powder is contained in an amount of 0.1 to 5.0% by weight.
The method according to claim 1,
The propolis extract is obtained by an ultrasonic extraction method.
delete 8. The method according to any one of claims 1 to 7,
Wherein the cosmetic composition is formulated with at least one selected from the group consisting of softening agents, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder.