KR101532001B1 - Cosmetics comprising fermented materials for preventing or improving skin wrinkle - Google Patents

Abstract

The present invention relates to a cosmetic product including fermented compositions and, more specifically, a cosmetic product for preventing or treating wrinkles including fermented compositions made by fermenting leaf of Tilia taquetii C. K. Schneid., lotus, Eucommia ulmoides OLIV. and Paeonia lactiflora and extracts of Portulaca oleracea L., herb and red ginseng wherein the cosmetic product comprises fermented compositions made by fermenting leaf of Tilia taquetii C. K. Schneid., lotus, Eucommia ulmoides OLIV. and Paeonia lactiflora twice with use of at least one strain selected from a group consisting of aspergillus, aspergillus oryzae, Aspergillus kawachii, Nattobacillus, Bacillus, lactic acid bacteria, yeast and actinomycetales.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for preventing or improving skin wrinkles containing a fermented composition,

The present invention relates to a cosmetic composition containing a fermented composition, and more particularly, to a cosmetic composition containing a fermented composition fermented with mulberry leaves, lotus leaves, mosses and peonies, a cosmetic composition for preventing or improving skin wrinkles containing herbal extracts, herbal extracts and red ginseng extract .

The cosmetic according to the present invention is characterized in that the mulberry leaves, the long leaves, the bugs, and the peony pests are classified into Aspergillus (Rhizopus), Aspergillus oryzae , white germ ( Aspergillus) kawachii), Meju fungus (hwanggok bacteria / hwanggyun / Nattobacillus), Bacillus subtilis (枯草菌/ Bacillus), lactic acid (乳酸菌/ Lactic Acid Bacteria), yeast (酵母菌/ Yeast), and Streptomyces (selected from the group consisting of放線菌) 1 Characterized in that it comprises a fermented composition prepared by primary fermentation and secondary fermentation with a strain of at least one strain.

Collagen, a major component of the extracellular matrix, is a major substrate protein in the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure.

Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). In most other structures, it exists as a fibrous inclusion. Collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis.

The main functions of collagen are known to be mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion or induction of cell division and differentiation (when organism grows or heals) (Van der Rest et al. Ann NY Acad Sci, 1990).

Such collagen is reduced by aging and photoaging by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin (Arthur K. Balin et al., Aging and the skin, 1989).

Particularly, collagen, which constitutes more than 70% of the skin dermis, is decomposed by a degrading enzyme called collagenase which is induced in ultraviolet rays and the like, which causes skin elasticity to deteriorate.

On the other hand, a product that helps wrinkle improvement is a cosmetic product that has the function of alleviating or improving the wrinkles of the skin by imparting elasticity to the skin, and the ultraviolet barrier cosmetic product has a function of protecting the skin from ultraviolet rays by blocking or scattering ultraviolet rays Cosmetics, and cosmetics that have the ability to smooth skin by preventing strong sunlight.

Currently, cosmetics with various functionalities are being developed and released, and consumer interest in functional cosmetics is also steadily increasing.

The ingredients of cosmetics can be roughly divided into 9 kinds such as water, oily raw material, surfactant, pigment, efficacy raw material, flavor, preservative, thickener or moisturizer.

Water usually corresponds to 'purified water' of cosmetic ingredients, which accounts for more than 10% of the product. Oily ingredients control the loss of moisture in the skin and improve absorption. Representative components include oils, waxes, higher fatty acids, higher alcohols, hydrocarbons, esters, and silicones.

Surfactant is a substance which adsorbs on the interface between two materials and changes its properties. It is composed of emulsifier which makes water and oil mixed well, solubilizer which makes small amount of oil soluble in water, dispersant which uniformly disperses solid particles in water, An external wetting agent, a foaming agent, a defoaming agent, and a detergent.

Moisturizing agent is a water-absorbing material that absorbs dry and keratinized skin and smoothes and softens the skin. Representative examples are glycerin, propylene glycol, butylene glycol, polyethylene glycol, sorbitol, sodium hyaluronate, and the like.

The thickener is used to maintain the viscosity or to maintain the stability of the product, and it is partially used as a moisturizer and a surfactant. Representative components include guar gum, gelatin, methyl cellulose, alginate, polyvinyl alcohol, and bentonite.

A pigment is a component that gives a color of a product such as a foundation or an eye shadow, and can be largely classified into a tar pigment, a natural pigment and an inorganic pigment.

Preservatives are used to prevent degeneration caused by microorganisms after opening the cosmetics. In total 60 kinds of preservatives that can be used in the country are used. Representative components include parabens, imidazolidinyl urea, phenoxyethanol, and phenonib.

Flavoring agent is a fragrance ingredient, and fragrance is not added to product which is fragrance-free, and fragrance of raw material itself can be made.

Efficacy Ingredients are effective ingredients that perform specific functions such as whitening, improving wrinkles, and feeling of firmness. It is supervised by the Food and Drug Administration to use the appropriate amount to maximize the efficacy without causing skin troubles.

As described above, cosmetics contain various chemical components, so the controversy over the safety of chemical components continues.

Concerns about the safety of some of the surfactants in cosmetics have been raised in several reports, and indeed some ingredients have been found to cause skin problems including dermatitis.

Parabens can cause skin irritation and contact dermatitis and show weak estrogen-like activity in animal studies.

In addition, the synthetic fragrances contain various allergen inducing antigens.

Cosmetics are not absorbed directly into the body like food, but are absorbed through skin and respiration. Therefore, there are not many cases that cause serious health hazards. However, since the age of use of cosmetics has been lowered and the kinds of cosmetics have been diversified, Efforts to secure the safety of cosmetics are becoming increasingly important due to many uses.

Since natural materials have fewer side effects and can be safely used than conventional chemical ingredients while retaining their functionality, studies using natural materials as active ingredients for functional cosmetics are actively under way.

The market for functional cosmetics using natural materials continues to grow. As of 2011, the share of herbal cosmetics in the domestic cosmetics market increased to 24% and occupied 60% of top-line cosmetics sales.

Fermented cosmetics are developed from the materials produced during the fermentation process of natural materials, and fermented the material to produce new effective ingredients, and at the same time, the molecular structure becomes smaller and quickly absorbed to the skin.

Various products are being developed by introducing fermentation techniques to various cosmetic ingredients, and the area of fermented cosmetics is increasing.

The present inventors have invented a cosmetic product which can prevent or improve skin wrinkles by producing a cosmetic product containing the fermented composition according to this trend.

Materials used in the present invention will be briefly described below.

Mulberry leaves are mulberry ( Morus alba L.), and the medicinal material with mulberry leaves is called mulberry leaf.

There is almost no odor, and the taste is sweet and spicy. Mulberry leaves contain glutamine, alanine, aspartic acid, tyrosine, serine, glutathione, minerals, dietary fibers and vitamins. Efficiency strengthens blood vessels, stabilizes blood pressure, and eliminates blood purification, heavy metal removal and aging It is known to have efficacy. It is also used for fever, headache, eye congestion, seawater, oozing, skin rash, and has been reported to have antidiabetic action by pharmacological action.

Peony (經药, Paeonia lactiflora ) is a perennial herbaceous herbaceous herbaceous peony peony and peony pupa, with a height of about 1 meter, and it can be divided into peony or vegetable depending on the flower color.

Peony contains ingredients such as aspartic acid and benzoic acid, and headache and toothache to settle. In addition, it removes the blood in the body, smoothes the blood circulation, cleanses the blood, and is effective in improving the female disease. When taking the peony pills steadily, it relaxes the gastrointestinal tract while suppressing the secretion of gastric acid, It is also effective for abdominal pain.

The Eucommia ( Eucommiae) ulmoides Oliver) is a medicinal herb dried bark.

Other names include 仲),,, 중),, 선, 思 사 사, 선,,,,, 棉,, 목 목, 棉,,,,, 선,,,,, 선,,,, and 絲 運 皮, It has a warm temperament.

It treats low back pain caused by lack of function of liver kidneys, knee pain, burning of the body, coldness of lower abdomen, frequent urination, sudden anxiety, uterine bleeding, blood pressure lowering effect, pharmacological action Blood pressure lowering, anti-aging, cholesterol lowering, anti-inflammation, sedation, pain relief, immune control, blood coagulation, uterine contraction, antiallergic and antibacterial activity.

The lotus leaf is called Nelumbo nucifera ), and it is also called lower leaf.

It is known that lotus leaves are rich in vitamins, dietary fiber, minerals, flavonoids, and quercetin, an antioxidant.

The lotus leaves are effective for depression of blood pressure, inhibition of aging due to antioxidative action, prevention of adult diseases, alleviation of dizziness or skin beauty.

On the other hand, Korean Patent Registration No. 10-1280483 discloses a functional cosmetic for improving skin wrinkles containing, as an active ingredient, a phosphorus-containing substance contained in a fermented extract as a prior art, as a prior art related to the present invention.

The present invention relates to a functional cosmetic composition for improving skin wrinkles containing a phosphorus-containing substance contained in a fermented extract of a genus, and more particularly to a functional cosmetic composition for improving skin wrinkles, Extracting and filtering it, filtering and concentrating it at a reduced pressure at 50 to 70 ° C and purifying it, and cultivating the above-obtained ginseng extract using Lactobacillus acidophilus to prepare a fermented jujube solution. And the like.

However, the prior art includes only one material, and the change of the physiologically active substance due to the fermentation process is not known, so that there is a problem that the safety of the fermentation product is unknown.

Korean Patent Laid-Open Publication No. 10-2009-0078084 also discloses a cosmetic composition for improving skin wrinkles containing a mulberry extract and adenosine.

This is because the mulberry extract promotes collagen synthesis in the skin and inhibits degradation, thereby improving wrinkles and elasticity of the skin. Adenosine accelerates DNA and protein synthesis in the dermis layer, thereby synergistically improving skin wrinkles and skin elasticity, However, since it does not include the fermentation process, the efficiency of the active ingredient may be lowered, and there is a problem that only the efficacy of the anti-wrinkle effect can be known.

Therefore, it is required to develop a cosmetic product which contains useful physiologically active substances through fermentation, has no toxicity, and has an effect of preventing or improving wrinkles of the skin.

Korean Patent Registration No. 10-1280483 (2013.06.25) Korean Patent Publication No. 10-2009-0078084 (July 17, 2009)

It is an object of the present invention to provide a cosmetic for preventing or improving skin wrinkles containing a fermented composition obtained by fermenting mulberry leaf, mulberry leaf, mulberry leaf and peony, mash extract, herbal extract and red ginseng extract.

The present invention has been made to solve the problems of the present invention by providing mulberry leaves, long leaves, bugs, and peonies with Aspergillus (Rhizopus), Aspergillus ( Aspergillus) oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast, and the like. And a fermentation composition prepared by primary fermentation and secondary fermentation with at least one strain selected from the group consisting of actinomycetes (actinomycetes).

The present invention aims at solving the technical problem by providing a cosmetic for preventing or improving skin wrinkles containing a fermentation composition obtained by fermenting mulberry leaves, long leaves, mosses and peonies, extracts of marigolds, herbal extracts and red ginseng extracts.

The present invention relates to a mulberry leaf, a mildew leaf, a mulberry leaf, and a peony pellet, which comprises Aspergillus (Rhizopus), Aspergillus oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast, and the like. And a fermented composition prepared by primary fermentation and secondary fermentation with one or more strains selected from the group consisting of actinomycetes (actinomycetes), to provide cosmetics for preventing or improving skin wrinkles.

The cosmetic product containing the fermented composition according to the present invention has a low side effect using natural products.

The cosmetic composition containing the fermented composition according to the present invention has an effect of preventing or improving wrinkles of the skin.

In addition, the cosmetic composition containing the fermented composition according to the present invention has the effect of decomposing the natural ingredient through the first fermentation and the second fermentation process, thereby easily absorbing the active ingredient into the skin.

FIG. 1 is a graph showing the results of measurement of cell viability after treatment with a natural product extract.
FIG. 2 is a table showing the results of measurement of collagenase inhibitory activity before and after fermentation of natural product extracts.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the experimental examples and the reference examples described in the present specification are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents and variations Examples should be understood.

The cosmetic according to the present invention is characterized in that it is prepared by adding a fermentation composition obtained by fermenting mulberry leaf, long leaf, bark, and peony.

In addition, it includes a mashin extract, a red ginseng extract, and a herbal extract. In addition, a moisturizing agent, an emulsifier, a surfactant, a preservative, a perfume, and the like commonly used in cosmetics are used.

The cosmetics according to the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sun cream, a foundation, Such as, but not limited to, soaps, shampoos, rinses, hair treatments, cosmetic wipes, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers, And may be all the goods used for.

Before examining the ingredients and manufacturing process of the cosmetic according to the present invention, the fermented composition contained in the cosmetic according to the present invention will be described.

The fermentation composition can be produced through Reference Example 1 or Reference Example 2. [

Reference Example 1. Method for producing fermentation composition

(1) Sterilization step

Mulberry leaves, peonies, bugs and lotus leaves are washed and sterilized.

Mulberry leaves, peony mosses, bugs, and lotus leaves can be those without any pretreatment, and can be prepared by cutting or grinding. Pretreatment methods such as cutting or grinding can also be selected from a variety of methods.

Preferably, the mulberry leaves, peony mosses, mites, and lotus leaves that have not been subjected to any pretreatment after being cleaned and removed are used.

Sterilization methods include physical methods using heat, ultraviolet rays, radiation or high frequency, and chemical methods using chemical fluids or gases, and a method of sterilization without damaging the active ingredients of mulberry leaves, peanuts, .

(2) Primary fermentation step

The fermentation strain is added to the mulberry leaves, peony moss, mulberry leaves and long leaves which have undergone the above sterilization step, and the first fermentation is carried out. Mulberry leaves, peonies, bugs, and lotus leaves fermented by fermentation strains can increase the extraction rate of medicinal components and can be easily absorbed into the skin.

Fermentation refers to a series of processes in which a complex organic compound is decomposed into a simple organic substance by using an enzyme contained in a microorganism. As the microorganism used in this step, Aspergillus, Rhizopus, Aspergillus oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast or Actinomycetes may be selected.

In the present invention, preferably Aspergillus (Rhizopus) or Aspergillus kawachii ), or a mixed microorganism thereof may be used, but the present invention is not limited thereto.

The fermentation may be carried out with mulberry leaves, peony mosses, mosses and orchid leaves, respectively. The mulberry leaves, peony mosses, peonies, mosses and oranges may be mixed and fermented.

According to the design conditions, the weight ratio of the mulberry leaves, peony mosses, peonies, and lotus leaves to the fermentation strain may be set differently, and the added amount of the fermentation strains may be 0.01 to 10 parts by weight based on 100 parts by weight of mulberry leaves, have.

The first fermentation may be performed at a temperature and a period of preferably from 20 to 70 ° C for 1 to 10 days, but the present invention is not limited thereto. In consideration of the characteristics of the fermentation strain, Suffice.

(3) Second fermentation step

The mulberry leaves, peonies, mulberry leaves and lotus leaves that have undergone the primary fermentation step are fermented second.

The fermentation strain, temperature and duration used in the secondary fermentation may be the same as the design conditions in the primary fermentation stage, but are not limited thereto.

Preferably, white germ can be used for the primary fermentation and can be used for the secondary fermentation.

(4) Extraction step

The fermented products of mulberry leaf, peony, mugwort and lotus leaf which have undergone the primary and secondary fermentation stages are dried and mixed and hydrothermally extracted.

Drying methods include natural drying, hot air drying, low temperature drying, freeze drying, far-infrared drying, daylight drying, vacuum drying, and polyethylene (PE) house drying.

Preferably, a natural drying method may be used, but not limited thereto, and it is sufficient to remove moisture from mulberry leaves, peony mosses, bugs, and lotus leaves.

At this time, the dried mulberry leaves, peony moss, moss, and lotus leaf can be used after being pulverized using a powder machine.

The fermented product of dried mulberry leaves, peonies, mulberry leaves and lotus leaves is mixed and hot water extracted to prepare a mixed extract.

At this time, the weight ratio can be variously set, but preferably the fermented product of mulberry leaf, peony, bark, and lotus leaf can be mixed at a weight ratio of 0.5-1.5: 0.5-1.5: 0.5-1.5: 0.5-1.5.

Various extraction methods such as hot water extraction method, solvent extraction method, steam distillation method, carbon dioxide supercritical extraction method, steam distillation method, microwave process extraction method and percolation extraction method can be used as a method of extracting mulberry leaves, peonies, , Preferably hydrothermal extraction, but not limited thereto

The temperature and time of the hot water extraction may be designed to suit the state of the fermented product, and preferably, it may be heated and extracted at 90 to 120 ° C for 1 to 3 hours.

At this time, when the hot water extraction condition is less than the range defined above, there is a possibility that the respective effective components contained in the fermented product may not be extracted sufficiently. If the hot water extraction condition exceeds the range defined above There is a possibility that each of the effective components contained in the fermentation product is destroyed and the efficacy thereof is lost.

(5) Filtration and concentration step

The combined extracts are filtered and concentrated.

The filtration methods include atmospheric pressure filtration, suction filtration, reduced pressure filtration, ultrafiltration, microfiltration and reverse osmosis filtration, and the like.

The filtration time, temperature and the number of times can also be set variously.

Filtration may be carried out using the methods listed above or other methods, and may be performed using two or more methods.

When performing microfiltration or ultrafiltration, the filtration membrane pore size may preferably be 3 μm, but is not limited thereto.

The concentration method includes, for example, freezing concentration, reduced pressure concentration, membrane concentration, vacuum concentration, heat concentration and evaporation concentration. Preferably, the concentration under reduced pressure can be performed, but is not limited thereto.

Two or more species may be used for the concentration method.

The concentration temperature can be set variously. But it is not limited thereto. The concentration time and the number of times can also be variously set.

Through the above steps, a fermentation composition containing mulberry leaves, lotus leaves, bugs, and peanuts as an active ingredient can be produced.

Reference Example  2. Manufacturing Method of Fermentation Composition

(1) Sterilization step of fermentation material

Was carried out in the same manner as described in Reference Example 1.

(2) Primary fermentation step

Was carried out in the same manner as described in Reference Example 1.

(3) Aging step

The fermented products of mulberry leaves, peonies, mulberry leaves and long leaves which have undergone the first fermentation step are respectively aged.

According to the design conditions, the aging temperature and duration may be aged for 10 to 90 days at 15 to 35 DEG C, but are not limited thereto.

The aging step may be performed after the first fermentation, but the present invention is not limited thereto, and the fermentation step may be performed after the first fermentation and the second fermentation step.

(4) Second fermentation step

Was carried out in the same manner as described in Reference Example 1.

(5) Ultrasonic wave breaking and extraction step

The fermented product of mulberry leaf, peony, bamboo, and lotus leaf after the second fermentation step is ultrasonicated.

The ultrasonic disruption time can be set to 15 to 60 minutes, and can be variously set according to the conditions.

The ultrasonic wave crusher has the effect of inducing molecular motion due to the unique cavitation of the ultrasonic wave and has the effect of improving the reactivity when performing the processes such as extraction and concentration by acting as activation energy in the molecular motion, When the sample is crushed using an ultrasonic wave crusher as an apparatus that has been introduced, the production rate of the protein contained in the sample can be improved.

In order to efficiently extract fermented water components of mulberry leaves, peony mosses, mulberry leaves and lotus leaves using the characteristics of the ultrasonic wave crusher as described above, ultrasonic disruption is performed on the fermentations of mulberry leaves, peony mosses, mosses and long leaves.

Extraction after ultrasonic disruption was carried out in the same manner as described in Reference Example 1.

(6) Filtration and concentration step

Was carried out in the same manner as described in Reference Example 1.

Reference Example  3. Functional effects of fermentation composition

2-1. Cell viability measurement

1) Experimental process

The toxicity of the natural extract was determined by measuring the cell viability using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) assay.

The MTT method can determine the cell survival rate by measuring the amount of MTT converted to formazan by the dehydrogenase contained in the mitochondria of living cells.

The cell line used was RAW 264.7 cells and cultured in DMEM (high glucose) medium supplemented with 10% FBS, penicillin (100 U / ml) and streptomycin (100 ug / ml)

① 96-well plate and then preparing six injected into the medium in each plate was inoculated 100 uL to be 5 × 10 ⁴ cell / well of RAW 264.7 cell, incubated in 5% CO _2, 37 ℃ incubator for 24 hours Respectively. One of the six plates was set as a control group and separated from the other plate.

(2) Natural extracts (200 μg / ml) were added to 5 plates except the plate set as a control group in the culture plate for 24 hours.

③ 15 μL of MTT solution was added to all wells and reacted at 37 ℃ incubator.

④ After 4 hours, insert the solubilizing solution, seal it to prevent the water from evaporating, leave it at 37 ℃ for 12 hours, measure the absorbance with a microplate spectrophotometer (Molecular dynamics, USA) using 570 nm and 630 nm as test and reference wavelength, respectively Respectively.

⑤ The OD of the control and experimental groups was compared and the cell viability was measured. The cell survival rate was calculated based on the following formula (1).

2) Experimental results

FIG. 1 is a graph showing the results of measurement of cell viability after treatment with a natural product extract.

As a result of the experiment, natural extracts showed cell viability similar to or higher than that of the control group, and thus it can be confirmed that there is no cytotoxicity.

2-2. Collagenase  Measurement of inhibitory activity

1) Experimental process

The collagenase activity inhibitory effect experiment was carried out according to the following method.

① 0.1 mg of collagenase (SIGMA C5138) was dissolved in 1 ml of 0.1 M Tris buffer (pH 7.1), and 0.5 mg of collagen (SIGMA C9879) was dissolved in 0.1 M Tris buffer

ris Buffer, pH 7.1), and samples were prepared for each concentration.

(2) 0.05 ml of the prepared sample, 0.05 ml of collagenase, and 0.4 ml of collagen were added to the test tube, followed by reaction at 37 ° C for 30 minutes in a water bath. Then, 1 ml of 25 mM citric acid solution was added to terminate the reaction.

③ The reaction tube was shaken with 5 ml of ethyl acetate, centrifuged at 3000 rpm for 5 minutes, and the absorbance of the ethyl acetate layer was measured at 320 ㎚.

Collagenase activity inhibition activity was calculated by the following equation, and the results are shown in FIG.

2) Experimental results

FIG. 2 is a table showing the results of measurement of collagenase inhibitory activity before and after fermentation of natural product extracts.

As a result, high collagenase inhibitory activity was measured in both the alcohol extraction and hot water extraction groups as compared with the positive control group.

2-3. Yield measurement of extract

The extraction efficiency according to the production method was examined by calculating the yield of the fermented composition according to Reference Examples 1 and 2.

1) Preparing the experiment

As the samples used in the experiments, the extracts according to Reference Examples 1 and 2 and the extracts obtained by directly extracting the plant material without fermentation were used as a comparative example.

2) Experimental process

Each extract was filtered with a vacuum filter, concentrated, lyophilized, and then the weight of the powder was measured to calculate the yield.

The yield was calculated using the following equation.

3) Experimental results

Table 1 shows the yield of the extract according to the production method.

Reference Example 1 Reference Example 2 Comparative Example Yield (% w / w) 11.34 17.81 7.07

As a result, the yield of the extract according to Reference Example 1 was 11.34%, the extract according to Reference Example 2 was 17.81%, and the extract according to Comparative Example 2 had a yield of 7.07%.

That is, it can be seen that the extraction yield of the material is increased by aging between the first fermentation and the second fermentation and extracting after ultrasonic disruption.

In summary, it can be confirmed that the fermentation composition obtained by fermenting mulberry leaves, lotus leaves, mites, and peonies has the effect of preventing or improving wrinkles of the skin, and cosmetics containing the fermentation composition also have the above-mentioned efficacy.

The cosmetics according to the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sun cream, a foundation, , A soap, a shampoo, a rinse, a hair treatment, a cosmetic wet tissue, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

The ingredients and preparation of cosmetics containing the fermented composition will be described in detail as follows.

Manufacturing example  1. Lotion containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycol, 2 to 4 parts by weight of glyceryl glucoside, And 0.1 to 7 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.15 parts by weight of carbomer and 0.01 to 0.15 part by weight of xanthan gum are added to the dissolved 1) solution and then dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 0.60 to 1.00 parts by weight of cetostearyl alcohol, 1.00 to 1.40 parts by weight of glyceryl stearate SE, 0.5 to 1.5 parts by weight of glyceryl stearate, 0.5 to 1.0 part by weight of Polysorbate 60, 0.3 to 0.7 part by weight of beeswax, 0.3 to 0.7 part by weight of Sorbitan Stearate, 5 to 7 parts by weight of Mineral Oil, 2 to 4 parts by weight of Macadamia Integrifolis Seed, 1 to 3 parts by weight of Caprylic / Capric Triglyceride, 0.03 to 0.07 part by weight of Tocopheryl Acetate and 0.03 to 0.07 part by weight of Dimethyl Silicone 0.20 to 0.60 parts by weight of oil) is melted by heating at 70 to 90 ° C, and then added to the above 2).

4) After charging, emulsify with homomixer at 3,000 rpm for 5 ~ 20 minutes.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.15 parts by weight of triethanolamide are added.

6) After the mixture is cooled to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added.

7) The fermented composition according to any one of the above items 1 to 5, wherein the fermented composition is 0.1 to 5 parts by weight, the herb extract is 0.01 to 0.05 parts by weight, the horseradish extract is 0.001 to 0.02 parts by weight, the red ginseng extract is 0.01 to 1 part by weight, the fragrance is 0.03 to 0.07 parts by weight, 0.5 to 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  2. Toner containing the fermentation composition

1) 70 to 80 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 part by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycol, 2 to 4 parts by weight of glyceryl glucoside, And 0.1 to 7 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.15 part by weight of carbomer is added to the dissolved solution (1), and the mixture is dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) Add 3 to 7 parts by weight of water aqua and 0.10 to 0.15 parts by weight of triethanolamide to the above 2).

4) 0.30 to 0.70 parts by weight of methylphenyl polysiloxane and 0.1 to 0.5 parts by weight of polysorbate 80 are weighed into the above 3).

5) 0.20 to 0.60 parts by weight of phenoxyethanol is added to 4) and mixed.

(6) The method of (5), wherein the fermented composition comprises 0.1 to 5 parts by weight of the herbal extract, 0.01 to 0.05 parts by weight of the herbal extract, 0.001 to 0.02 part by weight of the extract of marigold, 0.01 to 1 part by weight of the extract of red ginseng, 0.01 to 0.05 part by weight of fragrance, 7 parts by weight, and the mixture is mixed to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  3. Essence containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, , 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycols, and 1 to 3 parts by weight of glyceryl glucoside (weight ratio), 0.03 to 0.05 parts by weight of adenosine, 5 to 7 parts by weight of glycerine and 2 to 4 parts by weight of polyethylene glycol are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.10 parts by weight of carbomer and 0.10 to 0.15 part by weight of xanthan gum are added to the dissolved 1) solution and then dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 0.1 to 1.0 part by weight of Macadamia Integrifolia Seed and 0.05 to 0.15 part by weight of Methyl Phenyl Polysiloxane are metered into the above 2).

4) After cooling the mixture to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added and mixed.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.30 parts by weight of triethanolamide are added to 4).

6) A method for producing a fermented composition as described in 5) above, wherein the fermented composition is 0.1-5 parts by weight, the herb extract is 0.01-0.05 parts by weight, the gherkin extract is 0.001-0.02 parts by weight, the red ginseng extract is 0.01-1 parts by weight, the fragrance is 0.03-0.07 parts by weight and the hyaluronic acid 0.5 To 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  4. Cream containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of Niacinamide, 1.5 to 2.5 parts by weight of Butylene Glycol, 3 to 5 parts by weight of Glyceryl Glucoside, And 2 to 4 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.05 to 0.15 parts by weight of carbomer and 0.01 to 0.05 part by weight of xanthan gum are added to the solution 1) and dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 3 to 4 parts by weight of cetostearyl alcohol, 1 to 3 parts by weight of glyceryl stearate, 1 to 2 parts by weight of Polysorbate 60, 2 to 3 parts by weight of sorbitan 0.3 to 0.7 parts by weight of Sorbitan Stearate, 5 to 7 parts by weight of Mineral Oil, 2 to 4 parts by weight of Macadamia Integrifolis Seed, Caprylic / Capric triglyceride, 1 to 3 parts by weight of triglyceride, 0.03 to 0.07 part by weight of tocopheryl acetate, 0.20 to 0.60 part by weight of dimethyl silicone oil, 0.1 to 1.0 part by weight of microcrystalline wax, 1 to 2 parts by weight of stearic acid and 0.5 to 1.5 parts by weight of cyclopentasiloxane and dimethicone / dimethicone / vinyl dimethicone crosspolymer are weighed and dissolved by heating at 70 to 90 ° C., ).

4) After charging, emulsify with homomixer at 3,000 rpm for 5 ~ 20 minutes.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.20 parts by weight of triethanolamide are added.

6) After the mixture is cooled to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added.

7) The fermented composition according to any one of the above items 1 to 5, wherein the fermented composition is 0.1 to 5 parts by weight, the herb extract is 0.01 to 0.05 parts by weight, the horseradish extract is 0.001 to 0.02 parts by weight, the red ginseng extract is 0.01 to 1 part by weight, the fragrance is 0.03 to 0.07 parts by weight, 0.5 to 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

On the other hand, the present inventors conducted safety tests on the cosmetics according to Preparation Examples 1 to 4 and confirmed the results shown in Table 2 below.

Production Example 1. Lotion Production Example 2 Toner Production Example 3. Essence Production Example 4. Cream Appearance and Appearance White lotion phase Milky liquid
(Sensory evaluation) Opaque viscous liquid White creamy top Viscosity 6,000 ~ 8,000 2,500 ~ 3,500 15,000 ~ 20,000 pH 5.5 to 6.5 5.5 to 6.5 6.0 to 7.0 5.5 to 6.5 microbe General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) Common bacteria
0CFU / ml (not detected)
Pathogenic bacteria
0CFU / ml (not detected)

Claims (5)

A cosmetic product for prevention or improvement of skin wrinkles containing a fermented composition fermented with mulberry leaves, lotus leaves, persimmon and peony, herbal extract, herbal extract and red ginseng extract,
The fermentation composition is firstly fermented at 20 to 70 ° C for 1 to 10 days using Aspergillus kawachii , and after the first fermentation, the fermented composition is cultivated at 20 to 70 ° C using Aspergillus (Rhizopus) Lt; RTI ID = 0.0 > 70 C < / RTI > for 1 to 10 days,
Aspergillus kawachii or Aspergillus or Rhizopus is added in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of mulberry leaves,
Wherein the cosmetic product contains 0.1 to 5 parts by weight of a fermented composition, 0.001 to 0.02 parts by weight of a crude extract, 0.01 to 0.05 parts by weight of a herbal extract, and 0.01 to 1 part by weight of a red ginseng extract.
delete delete delete The method according to claim 1,
The cosmetic may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sunscreen, a foundation, Wherein the cosmetic composition is one of shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

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