KR102033448B1 - Composition for antiinflammation containing extract of soybean root - Google Patents
Composition for antiinflammation containing extract of soybean root Download PDFInfo
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- KR102033448B1 KR102033448B1 KR1020120083708A KR20120083708A KR102033448B1 KR 102033448 B1 KR102033448 B1 KR 102033448B1 KR 1020120083708 A KR1020120083708 A KR 1020120083708A KR 20120083708 A KR20120083708 A KR 20120083708A KR 102033448 B1 KR102033448 B1 KR 102033448B1
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- South Korea
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- composition
- soybean
- present
- bean
- root extract
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/304—Foods, ingredients or supplements having a functional effect on health having a modulation effect on allergy and risk of allergy
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 콩뿌리 추출물을 포함하는 항염증용, 항알러지용, 아토피 치료 또는 개선용, 보습용, 항균용 또는 모공 축소용 조성물에 관한 것이다. 본 발명의 조성물은 염증의 원인 물질의 생성을 저해 또는 억제시켜 염증을 치료 또는 완화시킬 수 있는 항염증 효과를 가지고, 알러지를 치료 또는 완화시킬 수 있어서 유용하다. 또한 본 발명의 조성물은 아토피를 치료하거나 개선시킬 수 있으며, 각질형성세포에서 분화를 촉진하여 보습 효과를 가지므로, 피부 건조증과 관련된 질환의 치료 또는 증상의 완화에 유용하다. 특히 본 발명의 조성물은 항염증능, 항알러지능, 아토피 치료 또는 개선능 또는 보습능에 있어서 콩 추출물보다 더 우수한 효과를 보인다. 본 발명의 조성물은 또한 천연물로부터 얻어지는 자연 유래의 추출물을 포함하여 생물체에 자극이 거의 없다. The present invention relates to an anti-inflammatory, anti-allergic, atopic treatment or amelioration, moisturizing, antimicrobial or pore reduction composition comprising soybean root extract. The composition of the present invention has an anti-inflammatory effect that can inhibit or inhibit the production of a causative agent of inflammation, thereby treating or alleviating inflammation, and is useful as it can treat or alleviate allergy. In addition, the composition of the present invention can treat or improve atopy, and has a moisturizing effect by promoting differentiation in keratinocytes, and thus is useful for treating or relieving symptoms associated with dry skin. In particular, the composition of the present invention shows a better effect than the soybean extract in anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.
Description
본 발명은 콩뿌리 추출물을 포함하는 항염증용, 항알러지용, 아토피 치료 또는 개선용, 보습용, 항균용 또는 모공 축소용 조성물에 관한 것이다. The present invention relates to an anti-inflammatory, anti-allergic, atopic treatment or improvement, moisturizing, antimicrobial or pore reduction composition comprising soybean root extract.
인간의 피부는 인체의 일차 방어막으로서 온도 및 습도의 변화, 자외선, 공해물질과 같은 외부 환경의 자극으로부터 체내의 기관을 보호해 주는 기능을 하며, 나이가 들어감에 따라 여러 가지 내적, 외적 요인에 의해 변화를 겪는다. 즉, 내적으로는 신진대사를 조절하는 각종 호르몬의 분비가 감소하고, 면역 세포의 기능과 세포들의 활성이 저하되어 생체에 필요한 면역 단백질 및 생체 구성 단백질들의 생합성이 줄어들게 되며, 외적으로는 오존층 파괴로 인하여 태양 광선 중 지표에 도달하는 자외선의 함량이 증가하고 환경 오염이 더욱 심화됨에 따라 자유 라디칼 및 활성 유해 산소 등이 증가함으로써, 피부의 두께가 감소하고, 주름이 증가하며, 탄력이 감소될 뿐 아니라 피부 혈색도 칙칙해지고, 피부 트러블이 자주 발생하며, 기미와 주근깨 및 검버섯 또한 증가하는 등 여러 가지 변화를 일으키게 된다.Human skin is the body's primary protective barrier, which protects the body's organs from changes in temperature and humidity, and from external environmental stimuli such as ultraviolet rays and pollutants. Undergo a change. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays that reach the surface of the sun's rays and intensifying environmental pollution, free radicals and free radicals increase, thereby reducing the thickness of the skin, increasing wrinkles, reducing elasticity, The color of the skin becomes dull, skin problems frequently occur, and there are various changes such as blemishes, freckles, and black mushrooms.
이러한 피부 내적 및 외적 요인에 의한 피부 상태의 변화를 방지하고, 건강한 피부 상태를 유지하기 위해서 기존에 알려진 각종 동물, 식물, 미생물 등으로부터 얻은 생리 활성 물질들을 화장품에 부가하여 사용함으로써 피부 상태를 개선시키기 위한 노력이 있어 왔다.In order to prevent changes in the skin condition caused by these internal and external factors and to maintain a healthy skin condition, the skin condition is improved by adding bioactive substances obtained from various known animals, plants, and microorganisms to cosmetics. Efforts have been made.
이에 본 발명자들은 콩뿌리 추출물이 항염, 항알러지 효과뿐만 아니라 아토피 증상의 개선 효과도 제공할 수 있으며, 피부 보습 기능 효과 등의 피부 상태 개선 효과를 제공할 수 있음을 확인하고 본 발명을 완성하게 되었다.Accordingly, the present inventors have confirmed that the soybean root extract can provide an anti-inflammatory, anti-allergic effect as well as an atopic symptom improvement effect, and can provide a skin condition improvement effect such as skin moisturizing function effect, and completed the present invention. .
본 발명의 목적은 항염증용, 항알러지용, 아토피 치료 또는 개선용 또는 보습용으로 이용할 수 있는 천연 추출물을 포함하는 조성물을 제공하는 데 있다. An object of the present invention is to provide a composition comprising a natural extract that can be used for anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing.
상기 목적을 달성하기 위하여, 본 발명은 콩뿌리 추출물을 유효성분으로 포함하는 항염증용 조성물을 제공한다.In order to achieve the above object, the present invention provides an anti-inflammatory composition comprising a soybean root extract as an active ingredient.
본 발명은 또한 콩뿌리 추출물을 유효성분으로 포함하는 항알러지용 조성물을 제공하고, 콩뿌리 추출물을 유효성분으로 포함하는 아토피 치료 또는 개선용 조성물 및 콩뿌리 추출물을 유효성분으로 포함하는 보습용 조성물을 제공한다. The present invention also provides an anti-allergic composition comprising soybean root extract as an active ingredient, a composition for treating or improving atopy comprising the soybean root extract as an active ingredient and a moisturizing composition comprising the soybean root extract as an active ingredient. to provide.
본 발명의 조성물은 염증의 원인 물질의 생성을 저해 또는 억제시켜 염증을 치료 또는 완화시킬 수 있는 항염증 효과를 가지고, 알러지를 치료 또는 완화시킬 수 있어서 유용하다. 또한 본 발명의 조성물은 아토피를 치료하거나 개선시킬 수 있고, 각질형성세포에서 분화를 촉진하여 보습 효과를 가지므로, 피부 건조증과 관련된 질환의 치료 또는 증상의 완화에 유용하며, 피부 가려움을 완화시킬 수 있다. 특히 본 발명의 조성물은 항염증능, 항알러지능, 아토피 치료 또는 개선능 또는 보습능에 있어서 콩 추출물보다 더 우수한 효과를 보인다. 본 발명의 조성물은 또한 천연물로부터 얻어지는 자연 유래의 추출물을 포함하여 생물체에 자극이 거의 없다. The composition of the present invention has an anti-inflammatory effect that can inhibit or inhibit the production of a causative agent of inflammation, thereby treating or alleviating inflammation, and is useful as it can treat or alleviate allergy. In addition, the composition of the present invention can treat or improve atopy, and has a moisturizing effect by promoting differentiation in keratinocytes, which is useful for the treatment or alleviation of symptoms associated with dry skin, and can relieve skin itching. have. In particular, the composition of the present invention shows a better effect than the soybean extract in anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.
본 명세서에서 '콩'은 그 종류에 제한이 없다. 본 발명의 일 관점인 조성물에 있어서, 구체적으로, 본 명세서의 콩은 서리태, 서목태, 흑태, 청태, 황태, 울타리콩, 강낭콩, 얼룩강낭콩, 적두, 거두, 콩나물콩 및 대두로 구성된 군으로부터 선택되는 어느 하나 이상을 포함할 수 있지만 이에 제한되는 것은 아니다. In the present specification, 'bean' is not limited in kind. In the composition which is one aspect of the present invention, specifically, the soybean of the present specification is selected from the group consisting of frosted, seomoktae, heuktae, chungtae, yellow, hedge bean, kidney bean, zebra kidney bean, red bean, soybean, bean sprout bean and soybean It may include any one or more, but is not limited thereto.
본 명세서에서 '콩뿌리'는 콩나무의 뿌리 부분을 의미한다. In the present specification, 'bean root' refers to the root portion of the bean tree.
본 명세서에서 '콩뿌리 추출물'은 추출과정에 있어서 예를 들면, 세척하고 건조시킨 콩뿌리 또는 이를 세말화한 콩뿌리 가루를 물 또는 유기용매에 넣고 추출하여 침적시킨 후 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 콩뿌리 추출물을 얻을 수 있다. 본 발명에 사용 가능한 유기용매는 에탄올, 메탄올, 부탄올, 에테르, 에틸아세테이트, 클로로포름 또는 이들 유기용매와 물의 혼합용매에서 선택될 수 있으며, 원료의 안전성을 고려할 때 바람직하게는 물 또는 30~70% 농도의 에탄올을 사용한다. 상기에서 용매를 이용하여 추출물을 얻은 이후 당업계에 알려진 통상적인 방법으로 상온에서 냉침, 가열 및 여과하여 액상물을 얻을 수 있으며, 또는 추가로 용매를 증발, 분무 건조 또는 동결 건조할 수 있으나, 이에 제한되는 것은 아니며, 당업계에서 일반적으로 사용되는 방법이라면 제한없이 사용하여 얻어질 수 있다.In the present specification, 'soybean root extract', for example, in the extraction process, washed and dried soybean root or powdered soybean root powder into water or an organic solvent, extracted and deposited, and then filtered through filter cloth and centrifugation. The residue and the filtrate are separated, and the filtrate is concentrated under reduced pressure to obtain a soybean root extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water or 30 to 70% concentration. Ethanol is used. After obtaining the extract using a solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying, but The present invention is not limited thereto, and any method generally used in the art may be obtained without any limitation.
본 발명은 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 항염증용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 피부 자극을 완화시키고, 염증을 완화시킬 수 있어서, 항염증용으로 사용할 수 있다. 구체적으로, 본 발명의 조성물은 프로스타글란딘 또는 인터루킨의 발현을 억제 또는 저해시키거나 또는 프로스타글란딘 또는 인터루킨의 활성을 억제 또는 저해시킴에 따라 항알러지 효과를 가져올 수 있다. The present invention relates to an anti-inflammatory composition comprising soybean root extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, can alleviate skin irritation and relieve inflammation, and thus can be used for anti-inflammatory. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin.
본 발명은 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 항알러지용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 알러지를 완화시키거나 또는 알러지의 원인이 되는 체내의 기작을 저해시켜서 알러지를 치료하거나 또는 완화시킬 수 있다. 구체적으로, 본 발명의 조성물은 프로스타글란딘 또는 인터루킨의 발현을 억제 또는 저해시키거나 또는 프로스타글란딘 또는 인터루킨의 활성을 억제 또는 저해시킴에 따라 항알러지 효과를 가져올 수 있다.The present invention relates to an anti-allergic composition comprising soybean root extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, may treat or alleviate allergy by alleviating allergy or inhibiting a mechanism in the body that causes allergy. Specifically, the composition of the present invention may have an antiallergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin or inhibiting or inhibiting the activity of prostaglandin or interleukin.
본 발명은 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 아토피 치료 또는 개선용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 항염증 및 항알러지 효과를 가질 뿐 아니라, 피부 장벽 기능을 강화시키고 피부 각질형성세포의 분화를 유도시킬 수 있어서, 피부 보호막의 이상 등이 원인이 되어 피부 건조증을 보이는 아토피의 치료 또는 아토피 증상의 완화를 위하여 사용할 수 있다. The present invention relates to a composition for treating or improving atopic dermatitis, comprising a bean root extract as an active ingredient in one aspect. The composition of an aspect of the present invention not only has an anti-inflammatory and anti-allergic effect, but also can enhance skin barrier function and induce differentiation of keratinocytes of the skin, resulting in abnormality of the skin's protective film and causing dry skin. It can be used for the treatment of visible atopy or for the relief of atopic symptoms.
본 발명은 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 보습용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 피부 장벽 기능을 강화시키고 피부 각질형성세포의 분화를 유도시켜서 피부의 수분을 증가킴에 따라 촉촉한 피부로 만들 수 있다. 따라서 본 발명의 일 관점인 상기 조성물은 표피 분화의 불완전함으로 생기는 피부건조증, 아토피 피부염, 접촉성 피부염 또는 건선 등을 예방 또는 개선하는 조성물로 유용하게 사용될 수 있다.The present invention relates to a moisturizing composition comprising soybean root extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, may enhance skin barrier function and induce differentiation of keratinocytes of the skin to increase the moisture of the skin, thereby making the skin moist. Therefore, the composition, which is an aspect of the present invention, may be usefully used as a composition for preventing or improving skin dryness, atopic dermatitis, contact dermatitis, or psoriasis caused by incomplete epidermal differentiation.
본 발명은 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 항균용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 구체적으로, 여드름균에 대하여 우수한 항균력을 보인다. 그러므로 상기 조성물은 여드름의 치료 및 완화에 있어서 효과적으로 사용가능하다. The present invention relates to an antimicrobial composition comprising soybean root extract as an active ingredient in one aspect. The composition of one aspect of the present invention, specifically, shows an excellent antimicrobial activity against acne bacteria. Therefore, the composition can be effectively used in the treatment and alleviation of acne.
본 발명은 다른 일 관점에서 콩뿌리 추출물을 유효성분으로 포함하는 모공 축소용 조성물에 관한 것이다. 본 발명의 일 관점인 상기 조성물은 구체적으로, 콜라겐의 생합성을 증가시켜, 모공의 크기를 축소시킬 수 있다. The present invention relates to a composition for reducing pores comprising soybean root extract as an active ingredient in another aspect. Specifically, the composition of the present invention may increase the biosynthesis of collagen, thereby reducing the size of the pores.
본 발명의 조성물은 각종 이유로 발생하는 피부 트러블 또는 피부 질환의 완화, 상태의 개선을 의미하거나 또는 피부 트러블 또는 피부 질환을 유발하는 각종 이유를 제거하거나 저해할 수 있고, 피부 외적인 상태 또는 이를 유발하는 피부 내적인 상태를 개선할 수 있어서 피부 상태를 개선시키는 데 유용하다. The composition of the present invention may mean alleviation of skin troubles or skin diseases occurring for various reasons, improvement of the condition, or removal or inhibition of various reasons causing skin troubles or skin diseases, and an external skin condition or skin causing them Internal condition can be improved, which is useful for improving skin condition.
본 발명의 조성물은 또한 모공 축소, 피지 조절 및 피부 트러블 개선용 조성물로서 사용될 수 있으며, 이는 피부에 적용 시 과잉으로 분비되는 피지를 억제하고, 활성 산소 제거와 콜라겐 합성을 촉진함으로써 모공을 축소시키며, 염증 인자의 발현 감소로 피부 트러블을 억제하는 효과가 탁월하다.The composition of the present invention can also be used as a composition for shrinking pores, regulating sebum and improving skin troubles, which suppresses excessive secretion of sebum when applied to the skin, shrinks pores by promoting free radical removal and collagen synthesis, Reducing the expression of inflammatory factors is excellent in inhibiting skin problems.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 조성물 총 중량에 대하여 0.001 내지 10 중량%의 콩뿌리 추출물을 포함할 수 있다. In one aspect of the present invention, the composition may comprise 0.001 to 10% by weight of the soybean root extract based on the total weight of the composition.
조성물 총 중량에 대하여 콩뿌리 추출물의 함량이 0.001중량% 미만이면 항염, 항알러지, 아토피 등의 피부 트러블 개선, 피부 보습 효과가 미미하고, 10중량%를 초과하면 함유량 증가에 따른 뚜렷한 효과의 증가가 나타나지 않기 때문이다. 상기와 같은 관점에 있어서, 본 발명의 조성물은 조성물 총 중량에 대하여, 0.005~9.5중량%, 0.01~9중량%, 0.03~8.5중량%, 0.05~8중량%, 0.07~7.5중량%, 0.09~7중량%, 0.1~6.5중량%, 0.3~6중량%, 0.5~5.5중량% 또는 0.7~5중량%의 콩뿌리 추출물을 포함할 수 있다.If the content of soybean root extract is less than 0.001% by weight based on the total weight of the composition, the skin troubles such as anti-inflammatory, anti-allergic and atopy are improved, and the skin moisturizing effect is insignificant. Because it does not appear. In view of the above, the composition of the present invention, 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to the total weight of the composition It may include 7% by weight, 0.1 to 6.5% by weight, 0.3 to 6% by weight, 0.5 to 5.5% by weight or 0.7 to 5% by weight of the bean root extract.
본 발명의 일 관점인 조성물에 있어서, 상기 콩은 서리태 (Seoritae, Glycin max MERR), 서목태 (Seomoktae, Rhynchosia Nolubilis), 흑태 (Black soybean, Glycine max(L.) Merr.), 청태 (blue bean, Glycime max MERR ), 황태 (yellow bean, Glycime max MERR), 울타리콩 (field bean, Vicia faba), 강낭콩 (kidney bean, Phaseolus vulgaris), 얼룩강낭콩 (pinto bean, Phaseolus vulgaris L.), 적두 (small red bean, Vigna angularis), 거두 (small black bean, Phaseolus angularis W.F. WIGHT. ), 콩나물콩 (sprouting bean, Glycine max (L.) Merr.) 및 대두 (soybean, Glycine max)로 구성된 군으로부터 선택되는 어느 하나 이상을 포함한다. In the composition of one aspect of the invention, the soybean is Seoritae ( Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr.), Cheongtae (blue bean, Glycime) max MERR), yellow bean, Glycime max MERR), field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean ( Glycine) max ) and any one or more selected from the group consisting of.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 프로스타글란딘의 생성을 억제 또는 저해시킬 수 있다. 본 발명의 일 관점인 상기 조성물은 프로스타글란딘 자체의 생성을 억제 또는 저해시킬 수 있거나 또는 프로스타글란딘의 활성을 억제 또는 저해시킬 수 있다. 본 발명의 일 관점인 상기 조성물은 또한 프로스타글란딘을 생성시키는 전단계 (up-stream)의 효소 또는 단백질의 활성을 억제 또는 저해시킬 수 있다. In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of prostaglandins. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of prostaglandin itself or may inhibit or inhibit the activity of prostaglandin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 인터루킨의 생성을 억제 또는 저해시킬 수 있다. 본 발명의 일 관점인 상기 조성물은 인터루킨 자체의 생성을 억제 또는 저해시킬 수 있거나 또는 인터루킨의 활성을 억제 또는 저해시킬 수 있다. 본 발명의 일 관점인 상기 조성물은 또한 인터루킨을 생성시키는 전단계 (up-stream)의 효소 또는 단백질의 활성을 억제 또는 저해시킬 수 있다. 구체적으로 상기 인터루킨은 인터루킨-6 또는 인터루킨-8을 포함할 수 있지만, 이에 제한되는 것은 아니다. In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of interleukin. The composition, which is an aspect of the present invention, may inhibit or inhibit the production of interleukin itself or may inhibit or inhibit the activity of interleukin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto.
본 발명의 일 관점인 조성물에 있어서, 상기 균은 여드름균을 포함한다. In a composition which is one aspect of the present invention, the bacterium includes acne bacteria.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 콜라겐의 생합성을 촉진시킬 수 있다. 상기 조성물은 콜라겐 자체의 생합성을 촉진시키거나 또는 활성을 증가시킬 수 있으며, 또는 콜라겐을 생성시키는 전단계 (up-stream)의 효소 또는 단백질의 활성을 증가 또는 촉진시킬 수 있다.In a composition which is one aspect of the present invention, the composition may promote biosynthesis of collagen. The composition may promote biosynthesis or increase activity of collagen itself, or increase or promote the activity of an enzyme or protein up-stream that produces collagen.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 화장료 조성물을 포함한다. In the composition which is one aspect of this invention, the said composition contains a cosmetic composition.
본 발명에 따른 피부 외용제 조성물을 화장료의 형태로 제형화 할 경우, 유연화장수, 수렴화장수, 영양화장수, 아이 크림, 영양 크림, 마사지 크림, 클렌징 크림, 클렌징 폼, 클렌징 워터, 파우더, 에센스 또는 팩 등의 형태로 제형화 될 수 있으며, 그 제형이 특별히 한정되는 것은 아니다. 또한, 본 발명에 의한 조성물은 지방 물질, 유기용매, 용해제, 농축제, 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제, 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품학 또는 피부과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 상기 보조제는 화장품학 또는 피부과학 분야에서 일반적으로 사용되는 양으로 도입된다. 또한, 본 발명의 조성물은 피부 개선 효과를 증가시키기 위하여 피부 흡수 촉진 물질을 함유할 수 있다.When formulating the external composition for skin according to the present invention in the form of cosmetics, soft cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack, etc. It may be formulated in the form of, the formulation is not particularly limited. In addition, the composition according to the present invention can be used for fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non- With ionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics It may contain adjuvants commonly used in the same cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.
본 발명에 따른 화장료 조성물은 상기한 물질 이외에 주 효과를 손상시키지 않는 범위 내에서, 바람직하게는 주 효과에 상승 효과를 줄 수 있는 다른 성분들을 포함할 수 있다. 또한 본 발명에 따른 화장료 조성물은 보습제, 에몰리언트제, 자외선 흡수제, 방부제, 살균제, 산화 방지제, pH 조정제, 유기 및 무기 안료, 향료, 냉감제 또는 제한제를 더 포함할 수 있다. 상기 성분의 배합량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 당업자가 용이하게 선정 가능하며, 그 배합량은 조성물 전체 중량을 기준으로 0.01 내지 5 중량%, 구체적으로 0.01 내지 3 중량%일 수 있다.The cosmetic composition according to the present invention may include other ingredients in addition to the above-mentioned substances within the range not impairing the main effect, preferably giving a synergistic effect to the main effect. In addition, the cosmetic composition according to the present invention may further include a moisturizer, an emulsifier, a UV absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavors, coolants or limiting agents. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 약학 조성물을 포함한다. In a composition that is one aspect of the invention, the composition comprises a pharmaceutical composition.
본 발명에 따른 조성물을 의약품에 적용할 경우에는, 상기 조성물을 유효성분으로 하여 상용되는 무기 또는 유기의 담체를 가하여 고체, 반고체 또는 액상의 형태로 경구 투여제 혹은 비경구 투여제로 제제화 할 수 있다. When the composition according to the present invention is applied to medicines, the composition may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier.
상기 경구 투여를 위한 제재로서는 정제, 환제, 과립제, 캡슐제, 산제, 세립제, 분제, 유탁제, 시럽제, 펠렛제 등을 들 수 있다. 또한, 상기 비경구 투여를 위한 제재로는 주사제, 점적제, 연고, 로션, 스프레이, 현탁제, 유제, 좌제, 패ㅊ 등을 들 수 있다. 본 발명의 유효성분을 제제화하기 위해서는 상법에 따라서 실시하면 용이하게 제제화할 수 있으며 계면활성제, 부형제, 착색료, 향신료, 보존료, 안정제, 완충제, 현탁제, 기타 상용하는 보조제를 적당히 사용할 수 있다.Examples of preparations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets and the like. In addition, the preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, patches and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspensions, and other commonly used auxiliaries can be suitably used.
본 발명에 따른 상기 약학 조성물은 경구, 비경구, 직장, 국소, 경피, 정맥 내, 근육 내, 복강 내, 피하 등으로 투여될 수 있다. The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.
본 발명의 약학 조성물의 유효 성분은 투여 받을 대상의 연령, 성별, 체중, 병리 상태 및 그 심각도, 투여 경로 또는 처방자의 판단에 따라 달라질 것이다. 이러한 인자에 기초한 적용량 결정은 당업자의 수준 내에 있으며, 이의 1일 투여 용량은 예를 들어 0.1mg/kg/일 내지 100mg/kg/일, 보다 구체적으로는 5 mg/kg/일 내지 50 mg/kg/일이 될 수 있으나, 이에 제한되는 것은 아니다.The active ingredient of the pharmaceutical composition of the present invention will depend on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one skilled in the art and its daily dosage may be, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.
본 발명의 일 관점인 조성물에 있어서, 상기 조성물은 건강식품 조성물을 포함한다. In a composition which is one aspect of the present invention, the composition comprises a health food composition.
본 발명에 따른 조성물은 포함하는 다양한 형태의 식품 첨가제 또는 기능성 식품을 제공한다. 상기 조성물을 포함하는 발효유, 치즈, 요구르트, 주스, 생균제제, 정제, 과립제, 드링크제, 캐러멜, 다이어트바 등으로 제형화될 수 있고, 통상적인 차 잎 형태나 티백 및 건강보조식품 등으로 가공될 수 있으며, 그 외 다양한 식품 첨가제의 형태로 사용될 수 있다. The composition according to the invention provides various types of food additives or functional foods comprising. Fermented milk, cheese, yogurt, juice, probiotic, tablets, granules, drinks, caramels, diet bars and the like containing the composition, and can be processed into conventional tea leaf form or tea bags and dietary supplements. And other various food additives.
일실시예에서 상기 조성물은, 본 발명이 목적으로 하는 주 효과를 손상시키지 않는 범위 내에서 주 효과에 상승 효과를 줄 수 있는 다른 성분 등을 함유할 수 있다. 예를 들어, 물성 개선을 위하여 향료, 색소, 살균제, 산화방지제, 방부제, 보습제, 점증제, 무기염류, 유화제 및 합성 고분자 물질 등의 첨가제를 더 포함할 수 있다. 그 외에도, 수용성 비타민, 유용성 비타민, 고분자 펩티드, 고분자 다당 및 해초 엑기스 등의 보조 성분을 더 포함할 수도 있다. 상기 성분들은 제형 또는 사용 목적에 따라서 당업자가 어려움 없이 적의 선정하여 배합할 수 있으며, 그 첨가량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 선택될 수 있다. 예를 들어, 상기 성분들의 첨가량은, 조성물 전체 중량을 기준으로, 0.01~5 중량%, 보다 구체적으로는 0.01~3 중량% 범위일 수 있다.In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The above ingredients may be suitably selected and blended by those skilled in the art according to the dosage form or purpose of use, and the amount may be selected within a range that does not impair the object and effect of the present invention. For example, the addition amount of the components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.
본 발명에 따른 조성물의 제형은 용액, 유화물, 점성형 혼합물, 타블렛, 분말 등의 다양한 형태일 수 있으며, 이는 단순 음용, 주사 투여, 스프레이 방식 또는 스퀴즈 방식 등의 다양한 방법으로 투여될 수 있다.The formulations of the compositions according to the invention may be in various forms, such as solutions, emulsions, viscous mixtures, tablets, powders, and the like, which may be administered by various methods such as simple drinking, injection, spray or squeeze.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다. Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.
[[ 제조예Production Example 1] 콩뿌리 추출물의 제조 1] Preparation of Soybean Root Extract
콩뿌리 1.5 kg을 믹서기로 분쇄한 뒤 80% 에탄올 수용액 7 ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 콩뿌리 추출물 216 g을 얻었다.
1.5 kg of soybean root was pulverized with a blender, and 7 L of an 80% ethanol aqueous solution was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 216 g of a bean root extract.
[[ 비교예Comparative example 1] 콩 추출물의 제조 1] Preparation of Soybean Extract
콩 1.5 kg을 믹서기로 분쇄한 뒤 80% 에탄올 수용액 7 ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 콩 추출물 252 g을 얻었다.
1.5 kg of beans were pulverized with a blender, and 7 L of an 80% ethanol aqueous solution was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 252 g of a soybean extract.
[[ 시험예Test Example 1] 염증 개선 효과 1] improvement of inflammation
1. 프로스타글란딘의 생성 억제 효과1. Inhibitory Effect of Prostaglandin Production
항염증 효과를 프로스타글란딘의 생성 억제 효과로 평가하였다. 콩뿌리 추출물과 비교예 1의 콩 추출물을 이용하여 대식세포를 대상으로 효과를 측정하였다. 우선, 마우스의 복강에서 채취한 대식세포에 최종농도가 500uM이 되도록 아스피린을 첨가해 세포에 잔존하는 시클로옥시제나제(cyclooxygenase, COX) 활성을 비가역적으로 억제하였다. 그런 다음 상기 현탁액을 96웰의 세포 배양관의 각 웰에 100μl를 넣어 5% CO2와 37℃ 조건의 배양기에서 2시간 동안 배양하여 대식 세포를 용기 표면에 부착시켰다. 이어, 부착된 대식 세포를 PBS로 3회 세척한 후 이를 염증 개선 효과 시험에 사용하였다. 상기 배양된 대식세포 5x104 세포/ml에 LPS를 1%(w/v)로 함유하는 RPMI 배지를 첨가하여 12시간 동안 배양한 후 프로스타글란딘의 생성을 유발하고 콩뿌리 추출물을 100μl 처리하여 유리된 프로스타글란딘을 효소면역 분석법(ELISA)을 이용하여 정량하였다.The anti-inflammatory effect was evaluated as the inhibitory effect of the production of prostaglandins. The effect was measured on macrophages using soybean root extract and soybean extract of Comparative Example 1. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 uM to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO 2 to attach macrophages to the container surface. Subsequently, the attached macrophages were washed three times with PBS and then used for the inflammation improvement effect test. Incubated for 12 hours by adding RPMI medium containing 1% (w / v) of LPS to the cultured macrophages 5x10 4 cells / ml to induce the production of prostaglandins and 100 μl of soybean root extract to free prostaglandins. Was quantified using an enzyme immunoassay (ELISA).
이때, 콩뿌리 추출물의 프로스타글란딘의 생성억제 활성은 LPS를 처리한 군과 처리하지 않은 군에서 각각 생성된 프로스타글란딘의 차이를 100%로 설정하고, LPS와 시료를 함께 처리하여 감소된 프로스타글란딘의 백분율을 통하여 대조군과 비교, 판정하였으며, 그 결과(프로스타글란딘의 생성억제 효과)를 하기 표 1에 나타내었다.At this time, the production inhibitory activity of the prostaglandin of the soybean root extract was set to 100% of the prostaglandin produced in the LPS-treated and untreated groups, respectively, and the LPS and the sample were treated together to reduce the percentage of prostaglandin. The results were compared with those of the control group, and the results were shown in Table 1 below.
상기 표 1에서 보는 바와 같이, 실험결과 아스피린으로 처리한 대조군과 같이 콩뿌리 추출물을 처리한 경우에도 프로스타글란딘의 생성 억제 효과가 매우 높음을 알 수 있다. 또한 콩 추출물에 비하여 콩뿌리 추출물은 프로스타글란딘의 생성 억제 효과가 더 우수함을 알 수 있다. 이를 통해 본 발명의 콩뿌리 추출물은 우수한 염증 개선 효과를 제공할 수 있음을 알 수 있다. 또한, 콩뿌리 추출물은 피부 염증 인자인 프로스타글란딘의 발현을 억제하여 피부 트러블을 방지하고 개선시킬 수 있음을 알 수 있다.
As shown in Table 1, it can be seen that even when the soybean root treated as the control group treated with aspirin, the effect of inhibiting the production of prostaglandins is very high. In addition, it can be seen that the soybean root extract is more excellent in inhibiting the production of prostaglandins than the soybean extract. It can be seen that the soybean root extract of the present invention can provide an excellent inflammation improving effect. In addition, it can be seen that the soybean root extract can prevent and improve skin trouble by inhibiting the expression of prostaglandin, a skin inflammatory factor.
2. 2. ILIL -8 생성 억제 효과-8 Production Suppression Effect
실험 하루 전 피부 각화상피세포(Normal human skin keratinocyte, NHEK, 입수처: Lonza)를 96웰(well) 플레이트에 5x104 세포/웰이 되도록 분주한 후 37℃, 5% CO2 인큐베이터(incubator)에서 24시간 동안 배양하였다. 24시간 후, PBS로 세포를 2회 씻어주고 무혈청 KBM(serum free keratinocyte basement media)으로 갈아주었다. 콩 추출물 50ppm과 각각의 웰에 콩뿌리 추출물을 하기 표 2의 농도별로 처리하여 30분간 반응시킨 후, PGSA(10㎍/㎖) 를 각각의 웰에 처리하였다. 여기서, PGSA(peptidoglycan from S. aureus )는 포도상구균에서 추출한 펩티도글리칸(peptidoglycan)으로서, 그람 양성(+) 균의 세포벽의 주요 구성 성분이고 박테리아의 세포막 성분들은 염증을 유발시키는 것으로 알려져 있으며, 특히 포도상구균의 경우 아토피 환자의 90% 정도가 이 균에 의한 2차 감염을 일으키는 것으로 보고되어 있다. 24시간 동안 37℃, 5% CO2 인큐베이터에서 배양한 후 배양액을 취하여 인터루킨-8(Interleukin-8, IL-8)에 대한 ELISA를 진행하였으며, 그 결과를 하기 표 2에 나타내었다. ELISA는 제조회사(BD science)의 실험 방법을 이용하였다.One day before the experiment, normal human skin keratinocytes (NHEK, obtained from Lonza) were dispensed into 96 well plates at 5x10 4 cells / well, and then in a 37 ° C., 5% CO 2 incubator. Incubated for 24 hours. After 24 hours, the cells were washed twice with PBS and changed to serum-free keratinocyte basement media (KBM). Soybean extract 50ppm and soybean root extract in each well was treated by the concentration of Table 2 and reacted for 30 minutes, PGSA (10㎍ / ㎖) was treated in each well. Here, PGSA (peptidoglycan from S. aureus ) is a peptidoglycan (peptidoglycan) extracted from Staphylococcus aureus, a major component of the Gram-positive (+) cell wall and bacterial membrane components are known to cause inflammation, In particular, in staphylococci, about 90% of patients with atopic dermatitis have been reported to cause secondary infection. After incubating for 24 hours at 37 ° C., 5% CO 2 incubator, the culture medium was taken and subjected to ELISA for Interleukin-8 (IL-8), and the results are shown in Table 2 below. ELISA used the experimental method of the manufacturer (BD science).
상기 표 2에서 콩뿌리 추출물이 PGSA와 LPS에 의해 증가한 IL-8의 분비를 현저히 감소 및 억제시키는 것을 확인할 수 있었다. 또한 콩 추출물과 비교하여도 현저히 높은 IL-8 분비 억제 효과를 확인 할 수 있었다. 따라서, 본 발명의 피부 외용제 조성물은 PGSA 의해 증가한 IL-8의 분비를 현저히 감소시킴으로써 우수한 항염증 효과를 제공할 수 있음을 알 수 있다.
In Table 2, it was confirmed that the soybean root extract significantly reduced and inhibited the secretion of IL-8 increased by PGSA and LPS. In addition, it was confirmed that the inhibitory effect of IL-8 secretion significantly higher than the soybean extract. Accordingly, it can be seen that the topical skin composition of the present invention can provide an excellent anti-inflammatory effect by significantly reducing the secretion of IL-8 increased by PGSA.
[[ 시험예Test Example 2] 가려움 완화 평가 2] itching relief assessment
실험 하루 전 각질형성세포(세포주명: HaCaT 입수처: ATCC)를 96 웰(well) 플레이트에 4x104세포/웰이 되도록 분주한 후 37℃, 5% CO2 인큐베이터(incubator)에서 24시간 동안 배양하였다. 24시간 후, HBSS(Hanks’Balanced Salt solution) 버퍼로 96 웰 플레이트를 2회 워싱(washing)한 후, 반응 버퍼(2μM Fluo-4-AM, 20% pluronic acid, 2.5mM probenecid)를 세포에 넣어주었다. 37℃, 5% CO2 인큐베이터에서 30분, 상온에서 30분간 반응시킨 후, HBSS 버퍼로 2회 워싱 하고 하기 표 3과 같은 농도(%)의 콩뿌리 추출물로 세포를 처리하였다. One day before the experiment, the keratinocytes (Cell name: HaCaT obtained from ATCC) were dispensed into 96 well plates at 4x10 4 cells / well, followed by incubation for 24 hours in a 37 ° C, 5% CO 2 incubator. It was. After 24 hours, wash 96 well plates twice with Hanks'Balanced Salt solution (HBSS) buffer, and then add reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) to the cells. gave. After reaction at 37 ° C., 5% CO 2 incubator for 30 minutes, and at room temperature for 30 minutes, the cells were washed twice with HBSS buffer and treated with soybean root extract at the concentrations (%) shown in Table 3 below.
10분간 반응시킨 후, 2U/ml 트립신(Trypsin) 또는 5μM PAR-2 활성 펩타이드(SLIGKV)를 처리하고 80초간 세포 내 Ca2 + 농도 변화를 측정하였다. 세포 내 Ca2 + 농도 변화 측정은 플렉스테이션3(FlexStation 3: Molecular Device, USA)를 이용하였다. 콩뿌리 추출물, 콩 추출물과 2U/ml 트립신(Trypsin) 또는 5μM PAR-2 활성 펩타이드(SLIGKV) 처리 후 80초간의 플렉스(flex)를 측정하여 얻어낸 값의 최소값과 최대값의 차를 구한 후, 그 값을 2U/ml 트립신 또는 5μM PAR-2 활성 펩타이드(SLIGKV) 처리 시의 최소값과 최대값의 차와 비교하여 칼슘 이온들의 세포 내 유입에 대한 억제율(%)을 하기 표 3에 나타내었다.After reaction for 10 minutes to process the 2U / ml trypsin (Trypsin) or 5μM PAR-2 activation peptide (SLIGKV) and was measured in Ca 2 + concentration in the cell 80 seconds. In Ca 2 + concentration in the measurement cell presentation flex 3: was used (FlexStation 3 Molecular Device, USA) . After the soy root extract, soybean extract and 2U / ml Trypsin or 5μM PAR-2 active peptide (SLIGKV) treatment, the flex was measured for 80 seconds, and the difference between the minimum and maximum values was obtained. The percent inhibition against intracellular influx of calcium ions compared to the difference between the minimum and maximum values treated with 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV) is shown in Table 3 below.
농도Test substance
density
상기 표 3에서 알 수 있듯이, 트립신 또는 PAR-2 활성 펩타이드(SLIGKV)에 의한 세포 내 칼슘 이온들의 유입이 콩뿌리 추출물의 처리에 따라 감소되며, 콩뿌리 추출물의 농도를 높여줌에 따라 세포 내 칼슘 이온들의 유입이 현저하게 감소됨을 확인할 수 있었다. 또한 콩 추출물과 비교 하여도 현저히 높은 칼슘 이온 유입 억제 효능을 확인 할 수 있다.As can be seen in Table 3, the influx of intracellular calcium ions by trypsin or PAR-2 active peptide (SLIGKV) is reduced with the treatment of soybean root extract, and the intracellular calcium ions as the concentration of soybean root extract is increased. It was confirmed that the influx of them was significantly reduced. In addition, compared to the soybean extract it can be confirmed that the effect of inhibiting calcium influx significantly higher.
따라서, 본 발명의 콩뿌리 추출물을 함유하는 피부 외용제 조성물은 가려움을 유발시키는 PAR-2 활성을 효과적으로 억제함으로써 우수한 항소양 효과를 제공할 수 있다.
Therefore, the external preparation composition for skin containing the soybean root extract of the present invention can provide an excellent antipruritic effect by effectively inhibiting PAR-2 activity causing itching.
[[ 시험예Test Example 3] 여드름균에 대한 항균력 시험 3] Antibacterial test for acne
하기 표 4에 나타낸 성분 및 함량(중량%)에 따라 제형예 1 및 비교제형예 1~3를 제조하였다. 구체적으로 설명하면, 제형예 1은 콩뿌리 추출물을 배합시킨 것이고, 비교제형예 1은 여드름 피부 개선의 유효성분을 전혀 포함시키지 않은 것이며, 비교제형예 2는 항균력에 대한 기준으로 삼을 표준물질로서, 여드름 치료제로 많이 사용하는 에리스롬마이신(erythromycin)을 함유시킨 것이다. 비교제형예 3은 콩 추출물을 배합시킨 제형이다.Formulation Example 1 and Comparative Formulation Examples 1 to 3 were prepared according to the ingredients and the contents (% by weight) shown in Table 4 below. Specifically, Formulation Example 1 is a blend of soybean root extract, Comparative Formulation Example 1 does not contain any active ingredients for acne skin improvement, Comparative Formulation Example 2 as a reference material to be used as a reference for antimicrobial activity It contains erythromycin, a popular acne treatment. Comparative Formulation Example 3 is a formulation containing a soybean extract.
제형예 1 및 비교제형예 1~3의 제조 방법은 다음과 같다. 하기 표 4의 A상의 성분들을 완전 용해시키고 별도의 용해조에서 B상의 성분들을 완전 용해시킨 다음, B상을 A상에 첨가하여 혼합 가용화 시켰다. 여기에 C상의 성분들을 표 4에 기재된 배합비율에 따라 첨가하여 혼합 균일화시킨 다음 여과시켜 본 조성물들을 제조하였다.The preparation method of Formulation Example 1 and Comparative Formulation Examples 1-3 is as follows. The components of phase A in Table 4 were completely dissolved and the components of phase B were completely dissolved in a separate dissolution bath, followed by mixing solubilization by adding phase B to phase A. The ingredients of phase C were added thereto according to the blending ratios described in Table 4 to homogenize the mixing and then filtered to prepare the compositions.
(hydrogenated castor oil)PEG-60 Cured Castor Oil
(hydrogenated castor oil)
제형예 1 및 비교제형예 1~3의 조성으로 제조된 각각의 화장료 조성물을 가지고 여드름 원인 균주인 프로피오니박테리움 아크네스(ATCC 6919: 배지-BHI 브로스(broth))에 대하여 항균력을 시험하였다.The antimicrobial activity was tested against propionibacterium acnes (ATCC 6919: medium-BHI broth), which is an acne causing strain, with each cosmetic composition prepared in the formulation of Formulation Example 1 and Comparative Formulation Examples 1-3.
여드름균에 대한 항균력 시험 방법은 다음과 같았다.The antibacterial test method for acne bacteria was as follows.
(1) 시험 균액 준비(1) Test bacteria preparation
프로피오니박테리움 아크네스는 BHI 브로스에 접종하여 혐기 배양한 배양액을 사용하였다.Propionibacterium acnes was used as a culture broth inoculated in BHI broth and anaerobic culture.
(2) 희석 용액 준비(2) dilution solution preparation
BHI 브로스(pH 6.8) 또는 LB 브로스(pH 4.5) 15ml에 상기 시험 균액을 0.15ml 첨가하여 잘 혼합한 것을 희석용액으로 사용하였다.0.15 ml of the test bacteria was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well as a dilution solution.
(3) 시료 준비(3) sample preparation
제형예 1 및 비교제형예 1~3로부터 제조된 화장료 조성물 원액 그대로를 시료로 사용하였다.Cosmetic compositions prepared from Formulation Example 1 and Comparative Formulation Examples 1 to 3 were used as samples.
(4) 항균력 시험(4) antimicrobial activity test
1) 96웰의 세포 배양관(96 well plate) 1번 행에 출발 농도에 맞도록 시료를 넣고 희석용액으로 총량을 200μl씩을 넣는다.1) Insert the sample to the starting concentration in the first well of 96-well cell culture tube (96 well plate) and add 200 μl of the total amount into the dilution solution.
2) 1번 행의 혼합액을 잘 섞어준 다음 100μl를 취하여 2번 행에 넣고 잘 섞어준 다음, 다시 100μl를 취하여 3번 행에 넣는 방식으로 이중 희석(double dilution)을 행한다.2) Mix the mixed solution in the first row well, take 100μl, put it in the second row, mix well, and then take 100μl and put it in the third row to perform double dilution.
3) 32℃에서 24시간 및 48시간 정치 배양한 후 현탁된 정도로 균의 증식 유무를 판단하여 균의 증식이 없는 최소농도를 MIC(최소저지농도; Minimum Inhibitory Concentration) 값으로 결정한다. 만약 혼합액이 불투명하여 균의 증식 유무를 판단하기 어려우면 현미경 관찰을 통하여 확인한다.3) After standing incubation for 24 hours and 48 hours at 32 ℃, determine the growth of bacteria to the extent of suspension to determine the minimum concentration without growth of bacteria as MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.
여드름균에 대한 항균력 시험 결과를 하기 표 5에 나타내었다. MIC는 제형에 함유된 유효성분의 농도로 환산하여 표기하였다.The antibacterial activity test results for acne bacteria are shown in Table 5 below. MIC is expressed in terms of the concentration of the active ingredient contained in the formulation.
MIC에서 ppm 농도가 작을수록 여드름균에 대한 항균력에 대하여 유효한 물질이라고 할 수 있는데, 제형예 1의 경우 공지의 여드름 치료제인 에리스롬마이신을 사용한 비교제형예 2보다 ppm 농도가 현저하게 작게 나와 콩뿌리 추출물을 함유하는 조성물은 시험균에 대하여 훨씬 우수한 항균력을 가짐을 확인할 수 있다. 또한 콩 추출물을 함유한 비교제형예 3에 비해 서도 현저히 높은 항균 효과를 확인 할 수 있다. The smaller the ppm concentration in MIC, the more effective the antimicrobial activity against acne bacteria. In the case of Formulation Example 1, the concentration of ppm was significantly lower than that of Comparative Formulation Example 2 using erythromycin, a known acne treatment. The composition containing the extract can be confirmed to have a much better antimicrobial activity against the test bacteria. In addition, compared to Comparative Formulation Example 3 containing soybean extract can be confirmed significantly higher antibacterial effect.
[[ 시험예Test Example 4] 모공축소 효과 4] pore reduction effect
본 발명의 조성물에 의한 조성물을 화장료로 사용하기 위하여, 콩뿌리 추출물과 콩 추출물을 함유하는 조성물을 하기 표 6에 명시된 조성으로 크림을 제조하였다.In order to use the composition according to the composition of the present invention as a cosmetic, a composition containing a soybean root extract and a soybean extract was prepared with a cream having the composition specified in Table 6 below.
베헤닐 알코올 & 소디움 스테아로일 락틸에이트Polyglyceryl-10 Pentastearate &
Behenyl Alcohol & Sodium Stearoyl Lactylate
1. 콜라겐 생합성 촉진을 통한 모공 축소 효과1. Pore reduction effect through promoting collagen biosynthesis
본 발명에 의한 콩뿌리 추출물의 콜라겐 생합성 촉진 효과를 TGF-β와 비교하여 측정하였다. 먼저, 섬유아세포(fibroblast)를 24 공(well)에 1 공 당 105개씩 파종(seeding)하여 90% 정도 자랄 때까지 배양하였다. 이를 24시간 동안 무혈청 DMEM 배지로 배양한 후 무혈청 배지에 녹인 본 발명의 콩뿌리 추출물, 콩 추출물과 TGF-β를 각각 10㎎/㎖씩 처리하고 CO2 배양기에서 24시간 동안 배양하였다. 이들의 상층액을 떠내어 프로콜라겐 형(I) ELISA 키트(procollagen type(I))를 이용하여 프로콜라겐 (procollagen)의 증감여부를 보았다. 그 결과를 하기 표 7에 나타내었으며, 콜라겐의 합성능은 비처리군을 100으로 하여 대비하였다.Collagen biosynthesis promoting effect of the soybean root extract according to the present invention was measured in comparison with TGF-β. First, fibroblasts were seeded by 10 5 per hole in 24 wells and cultured until 90% growth. After incubation for 24 hours in serum-free DMEM medium and treated with soybean root extract, soybean extract and TGF-β of the present invention dissolved in serum-free medium, respectively 10mg / ㎖ and CO 2 The incubator was incubated for 24 hours. These supernatants were removed and procollagen increased or decreased using procollagen type I ELISA kit (procollagen type (I)). The results are shown in Table 7 below, and the synthetic ability of collagen was compared with the non-treated group as 100.
상기 표 7의 결과에서, 본 발명에 의한 콩뿌리 추출물은 양성대조군인 TGF-β 과 비슷한 콜라겐 합성능을 나타내는 것을 확인할 수 있었다. 또한 콩 추출물에 비해서도 현저한 콜라겐 합성 효과를 확인 할 수 있다. 따라서, 본 발명에 의한 콩뿌리 추출물이 모공 주변의 콜라겐 생성량을 증가시켜 넓어진 모공을 축소시킬 수 있음을 확인하였다.In the results of Table 7, the soybean root extract according to the present invention was confirmed to exhibit a collagen synthesis similar to the TGF-β positive control group. In addition, compared to the soybean extract can be confirmed a significant collagen synthesis effect. Therefore, it was confirmed that the soybean root extract according to the present invention can reduce the enlarged pores by increasing the amount of collagen production around the pores.
2. 모공 축소 효과2. Pore Reduction Effect
제형예 2 및 비교제형예 4~5의 모공 축소 효과를 알아보기 위하여 다음과 같이 평가하였다. 모공 크기가 넓은 피험자 남녀 30명을 선정하여 10명씩 3개 군으로 나누고 각 군별로 얼굴에 제형예 2 및 비교제형예 3의 영양크림을 4주간 매일 바르게 하였다. 모공 축소의 효과에 대한 판정은 실험 전과 4주 후 사진을 찍어서 전문가들의 육안 평가로 이루어졌다. 그 결과는 하기 표 8에서 나타내었다. (평가 등급: 0 - 전혀 축소 되지 않았다; 5 - 매우 축소되었다)In order to determine the pore reduction effect of Formulation Example 2 and Comparative Formulation Examples 4 to 5 were evaluated as follows. Thirty male and female subjects with large pore sizes were selected and divided into three groups of ten, and the nutritional creams of Formulation Example 2 and Comparative Formulation Example 3 were applied to the face daily for 4 weeks. Determination of the effect of pore reduction was made by visual assessment by experts, taking pictures before and after 4 weeks of the experiment. The results are shown in Table 8 below. (Rating: 0-did not shrink at all; 5-very reduced)
상기 표 8의 결과로부터, 비교제형예 4~5은 모공 축소 효과가 거의 없지만, 제형예 2의 경우에는 육안으로 확인 가능할 정도의 모공 축소 효과를 나타내어 본 발명에 의한 콩뿌리 추출물은 모공의 크기를 감소시키는 효과가 우수함을 알 수 있었다.From the results of Table 8, Comparative Formulation Examples 4 to 5 have little pore shrinkage effect, but in the case of Formulation Example 2 exhibited a pore shrinkage effect that can be seen with the naked eye, soybean root extract according to the present invention is the size of the pores It was found that the reducing effect is excellent.
이하, 본 발명에 따른 조성물의 제형 예를 설명하나, 약학 조성물 및 화장료 조성물은 여러 가지 제형으로 응용 가능하며, 이는 본 발명을 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, the formulation examples of the composition according to the present invention, but the pharmaceutical composition and cosmetic composition is applicable to a variety of formulations, which is intended to explain in detail only, not intended to limit the present invention.
[제제예 1] 연질캅셀제Preparation Example 1 Soft Capsule
콩뿌리 추출물 150 mg, 팜유 2 mg, 팜경화유 8 mg, 황납 4 mg 및 레시틴 6 mg을 혼합하고, 통상의 방법에 따라 1 캡슐당 400 mg씩 충진하여 연질캅셀을 제조하였다.Soybean root extract 150 mg, palm oil 2 mg, palm hardened oil 8 mg, lead 4 mg and lecithin 6 mg were mixed, and 400 mg per capsule was filled according to a conventional method to prepare a soft capsule.
[제제예 2] 정제Preparation Example 2 Tablet
콩뿌리 추출물 150 mg, 포도당 100 mg, 홍삼추출물 50 mg, 전분 96 mg 및 마그네슘 스테아레이트 4 mg을 혼합하고 30% 에탄올을 40 mg 첨가하여 과립을 형성한 후, 60℃에서 건조하고 타정기를 이용하여 정제로 타정하였다.150 mg of soybean root extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules. Tableting.
[제제예 3] 과립제Preparation Example 3 Granule
콩뿌리 추출물 150 mg, 포도당 100 mg, 홍삼추출물 50 mg 및 전분 600 mg을 혼합하고 30% 에탄올을 100 mg 첨가하여 과립을 형성한 후, 60℃에서 건조하여 과립을 형성한 다음 포에 충진하였다. 내용물의 최종 중량은 1 g으로 하였다.150 mg of soybean root extract, 100 mg of glucose, 50 mg of red ginseng extract, and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules. The granules were dried at 60 ° C. to form granules. The final weight of the content was 1 g.
[제제예 4] 드링크제[Example 4] Drinks
콩뿌리 추출물 150 mg, 포도당 10 g, 홍삼추출물 50 mg, 구연산 2 g 및 정제수 187.8 g을 혼합하고 병에 충진하였다. 내용물의 최종 용량은 200 ml로 하였다.150 mg of soy root extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled into bottles. The final dose of the contents was 200 ml.
[제제예 5] 건강 식품의 제조Preparation Example 5 Preparation of Health Food
콩뿌리 추출물 .............................. 1000 ㎎ Bean Root Extract .............. 1000 mg
비타민 혼합물 Vitamin mixtures
비타민 A 아세테이트.......................70 ㎍ Vitamin A Acetate ......... 70 μg
비타민 E ............................................ 1.0 ㎎ Vitamin E ......................................... 1.0 mg
비타민 B1........................................... 0.13 ㎎ Vitamin B1 ..................... 0.13 mg
비타민 B2 .......................................... 0.15 ㎎ Vitamin B2 ......................................... 0.15 mg
비타민 B6........................................... 0.5 ㎎ Vitamin B6 ......................................... 0.5 mg
비타민 B12......................................... 0.2 ㎍ Vitamin B12 ......................... 0.2 μg
비타민 C............................................. 10 ㎎ Vitamin C ......................................... 10 mg
비오틴.................................................. 10 ㎍ Biotin ... 10 ㎍
니코틴산아미드.................................. 1.7 ㎎ Nicotinic Acid Amide ...................................... 1.7 mg
엽산...................................................... 50 ㎍ Folic Acid ... ..... 50 ㎍
판토텐산 칼슘.................................... 0.5 ㎎ Calcium Pantothenate ............... 0.5 mg
무기질 혼합물 Mineral mixture
황산제1철.......................................... 1.75 ㎎ Ferrous Sulfate ......................................... 1.75 mg
산화아연.............................................. 0.82 ㎎ Zinc Oxide ......................................... 0.82 mg
탄산마그네슘...................................... 25.3 ㎎ Magnesium Carbonate ......................................... 25.3 mg
제1인산칼륨.......................................... 15 ㎎ Potassium monophosphate ......................................... 15 mg
제2인산칼슘.......................................... 55 ㎎ Dibasic Calcium Phosphate ......................................... 55 mg
구연산칼륨............................................ 90 ㎎ Potassium Citrate ......................................... 90 mg
탄산칼슘.............................................. 100 ㎎ Calcium Carbonate ......................................... 100 mg
염화마그네슘..................................... 24.8 ㎎ Magnesium Chloride ......................................... 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 건강식품에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강식품 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강식품 조성물 제조에 사용할 수 있다.Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.
[제제예 6] 건강 음료의 제조 Preparation Example 6 Preparation of Healthy Drinks
콩뿌리 추출물............................ 1000 ㎎ Soybean Root Extract ...................................... 1000 mg
구연산..................................................... 1000 ㎎ Citric Acid ... .... 1000 mg
올리고당..................................................... 100 g oligosaccharide................................................. .... 100 g
매실농축액..................................................... 2 g Plum concentrate ..................... ..... 2 g
타우린............................................................ 1 g Taurine ... ........... 1 g
정제수를 가하여 전체......................... 900 ㎖ Purified water is added to the whole ........... 900 ㎖
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1시간 동안 85℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2ℓ용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 건강음료 조성물 제조에 사용한다.After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained by sterilization in a sterilized 2 L container sealed sealed sterilized and then stored in the present invention For the preparation of healthy beverage compositions.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만 수요계층이나, 수요국가, 사용 용도 등 지역적, 민족적 기호도에 따라서 그 배합비율을 임의로 변형 실시하여도 무방하다. 본 발명이 속한 기술 분야에서 통상의 지식을 가진 자라면 상기 내용을 바탕으로 본 발명의 범주 내에서 다양한 응용 및 변형을 행하는 것이 가능할 것이다. Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose. Those skilled in the art to which the present invention pertains will be able to perform various applications and modifications within the scope of the present invention based on the above contents.
[제형예 3] 유연화장수(스킨로션)[Formulation Example 3] Softening Cosmetic (Skin Lotion)
[제형예 4] 영양화장수(밀크로션)Formulation Example 4 Nutrients (Milk Lotion)
[제형예 5] 영양크림Formulation Example 5 Nutrition Cream
[제형예 6] 마사지 크림Formulation Example 6 Massage Cream
[제형예 7] 팩[Formulation Example 7] Pack
[제형예 8] 연고Formulation Example 8 Ointment
이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적 기술은 단지 바람직한 실시태양일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.As described above in detail the specific parts of the present invention, it is apparent to those skilled in the art that such specific description is merely a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
Claims (15)
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