KR102236392B1 - A Producing method of cosmetic compostion including extracts of fig leaves - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fig alcohol extract. Specifically, the fig alcohol extract, as further comprising a herbal medicine, persimmon extract, evening primrose seed oil saponified product, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract; The fig leaf alcohol extract has antioxidant, anti-inflammatory and whitening activities; The herbal medicine is made by finely powdering almonds, jullmu, yulpi, doin, baekgangjam, tosaja, mint, baekpyeon, white paper and mugwort; The persimmon extract is extracted from persimmon peel and persimmon, and is obtained by using a distillation method; The evening primrose seed oil saponified product is saponified by treating the evening primrose seed oil with an alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide, and potassium hydroxide; The sorghum powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 10% by weight to 10% by weight of continuous pulverization for 48 to 72 hours without alkali treatment; The ginseng extract refers to a substance in which a specific ginseng saponin substrate is increased by fermenting ginseng; The mung bean extract is obtained by immersing mung bean powder in an aqueous alcohol solution, and contains bitexin and isobitexin as active ingredients. It relates to a cosmetic composition comprising a fig leaf alcohol extract.

Description

A producing method of cosmetic composition including extracts of fig leaves}

The present invention relates to a cosmetic composition comprising a fig leaf alcohol extract, and in particular, a herbal medicine, persimmon extract, evening primrose seed oil saponified product, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract And it relates to a cosmetic composition and a method of manufacturing the same further comprising mung bean extract.

Figs are called figs because they do not bloom, but in reality, flowers bloom inside the fruit, so they do not appear to the outside. The pulp is an alkaline fruit with a lot of protein and fiber, with the development of flower buds and ovaries. Since it is a unit fruit, it does not require moisture. Fruits are mainly reproductive, but they are mainly distributed as canned or dried fruits because they do not have storage and transport properties. Varieties are divided into four lines: Capri type, Sumiruna type, San Pedro type, and normal type. Korea mainly cultivates normal type and San Pedro type.

Fig is a subtropical semi-arid deciduous broad-leaved fruit tree native to the western and Mediterranean coasts of Asia. It is easy to cultivate and has little damage from pests and pests. Cultivation is possible only in areas with an annual average temperature of 15℃ and winter temperatures of -9℃ or higher. The cultivation history in Greece and Italy is long worldwide, and Korea was introduced through Japan and China, and the current cultivation centers are in California, Italy, Turkey, and Portugal, and in particular, Italy and Greece account for about 2/3 of the world's total production. Occupies. Currently, economically cultivated places in Korea are limited to some areas of Jeollanam-do, and they are mainly planted and inhabited at home, and are cultivated in Jeju Island and the southern coastal areas.

These figs contain various nutrients such as niacin, sodium, protein, sugar, retinol, beta-carotene, vitamins A, B1, B2, B6, C, E, dietary fiber, zinc, folic acid, phosphorus, lipids, iron, potassium, and calcium. It is a plant that is in the spotlight for food science and pharmaceuticals. And it has been known quite a bit when these ingredients are extracted and applied to cosmetics.

Herbal medicines such as passerby, adlay, yulpi, doin, baekgangjam, tosaja, peppermint, baekpyeong, white paper, and wormwood have been well used in oriental medicine, and have been proven many times to have a pharmacological effect on the extract, and today not only in oriental medicine. It is also a popular ingredient in the health functional food and cosmetics industry. And there have been cases of using ingredients extracted from these herbal medicines in cosmetics.

Persimmon is an alkaline fruit rich in sugars such as glucose and fructose. It is rich in tannic acid, which gives off astringent taste, and the content of vitamin C is about 5-20 times higher than that of apples in the same amount and rich in minerals. In addition, gamgotji (Diospyros kaki calyx) is a herbal name and is called corpse. In folk remedies, it is known to be effective in treating cough, asthma, chronic bronchitis, stopping scabies, enuresis, and blood pressure enhancement. It is known to have excellent whitening effect by inhibiting expression and inhibiting melanin production.

In addition, the tannin and proanthocyanidin components contained in the persimmon shell are water-soluble substances, and are known for their physiological activities such as antibacterial, antioxidant, anti-tumor and heavy metal removal, and the polyphenol component of the persimmon shell is antioxidant. And it is known to have an antigenotoxic effect.

However, it is common to consume only persimmon flesh and discard persimmons and persimmons, and some are used as feed or secondary processing, but recycled use is only a small fraction, and most persimmons and persimmons A significant amount of silver is being discarded.

Evening primrose oil was used by Native Americans as a medicine to treat wounds or to treat colds, and recently, it is consumed as a cosmetic or nutrient. In addition, evening primrose oil is rich in gammalinolenic acid and linolenic acid, which has various health benefits such as improving vascular disease, lowering cholesterol levels, controlling female hormones, and preventing adult diseases. Gamminolenic acid is a type of unsaturated fatty acid, Omega 6, and it is known that it is not a component produced by our body. If you have skin conditions such as atopy or skin psoriasis, it is also helpful to apply evening primrose oil directly to your skin.

Sorghum is 1m long, butterfly is 5cm long, leaves are large, middle ribs are white and clear, leaves have no leaves, and leaves are blackish brown hwanjangmakpyeon. It is a common plant distributed throughout the country with nappies. It is a food with anticancer and antioxidant effects. It is widely used in the pharmaceutical or cosmetic industry.

Pork potato is a naturalized plant, and although it is not known exactly, it is believed to have been transmitted from Europe to China after the 17th century, and grows wildly in fields and fields. If you dig under the dry stem, you can find tubers of pork potato, and the flowers blooming in autumn are very attractive. Nowadays, as it is known to be good for diabetes, an increasing number of farmers harvest or cultivate wild produce and sell it.

Cacao is also known as cocoa, and is native to the tropical regions of the Americas, with a thick stem and a height of 12m. The fruit has a long oval shape, is about 10cm long, is divided into 5 pieces, and contains 40~60 seeds. Seeds have no endosperm and have enlarged cotyledons. Seeds contain about 2% theobromin, some caffeine, and 50% fat. When seeds are removed from ripe fruits and fermented for several days in a wooden barrel, the seeds turn reddish brown and give off a unique scent. The cacao beans are washed with water and dried, and the cacao paste is roasted and made into powder. Chocolate is hardened by adding sugar, milk, and spices. Cocoa is the fat extracted by compressing cacao paste, and the fat obtained from this is called cacao butter. Cacao butter is transparent yellow and has a unique scent and flavor, so it is used in margarine, pomade, and pharmaceuticals. Theobromine is a type of alkaloid and is used as a diuretic because it has a strong effect on the kidneys. It has been cultivated since BC, and is now cultivated not only in the Americas, but also in tropical regions such as West Africa, Java, India and Sri Lanka.

Ginseng is the broadest concept that includes fresh ginseng, white ginseng, red ginseng, wild ginseng, camphor, misam, and won ginseng. Ginseng is a natural material that has been used as a very valuable medicine in oriental medicine. The main functional ingredient of ginseng is ginseng saponin, called "ginsenoside," which is specifically named only ginseng saponin among various saponins in plants. The main components of ginseng are ginsenosides, which are saponins, and ginsenosides Rb1, Rb2, Rc, which are protopanaxadiol, and ginsenosides Re, Rg1, and Rf, which are protopanaxatriol are known. Representative pharmacological actions of these ingredients include anti-cancer activity, anti-inflammatory, and anti-diabetic action. In addition, domestic and foreign studies have recently been conducted on minor ginsenosides such as Rh2, Rg3, C-K, F2, etc., which are considered metabolites of the above ginsenosides. However, since the method to obtain minor ginsenosides is difficult, further studies on the pharmacological effect are insufficient, and the comparison of the pharmacological effects of each of the various ginsenosides is still insufficient.

Mung bean (Vigna Radiata (L.) Wilczek or Mungbean) has been widely used as an antipyretic or detoxifying agent in oriental medicine since ancient times. It is effective in removing spots, removing heat rash that occurs well in summer, and being effective in swelling, boils, and mumps caused by penetration of skin filamentous bacteria into the stratum corneum of the skin. The skin care effect of mung beans is due to vitexin and isovtexin contained in mung beans, and these physiologically active substances are a kind of flavonoid-C-glycoside. Bitexin and isobitaxin components are known to be effective in preventing the accumulation of waste products in the skin and inhibiting aging because they have the function of removing free radicals, which are toxic components in the body, and sebum from the skin. As such studies on bitexin and isobitaxin, a method of separating the active substance contained in the ethanol extract of mung bean or separating the bitexin and isobitaxin contained in the water extract of mung bean has been reported, and other natural substances other than mung bean As a method of separating bitexin from lichens, isobitaxin from rice ethanol extract, and vitaxin and isobitaxin from buckwheat ethanol extract has been reported.

There have been many cases of using ingredients extracted from various natural products, but in the present invention, in particular, with the focus on the antioxidant, anti-inflammatory and whitening activities of fig leaf alcohol extract, synergistic effect when combined with the above various ingredients compared to when used alone. Revealed that there is.

As mentioned above, an object of the present invention is to provide a cosmetic composition containing various natural extract components and a method for producing the same, which have been found out in the prior art, focusing on the antioxidant, anti-inflammatory and whitening activities of the fig leaf alcohol extract.

In addition, it is an object of the present invention to provide a cosmetic composition having a synergistic effect and a method of manufacturing the same when several components are mixed compared to a single composition of various natural extract components disclosed in the prior art.

As a means for solving the above problems, an embodiment according to the present invention is fig leaf alcohol / ethanol extract; Mixing herbal medicines, persimmon extract, evening primrose oil saponification, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract with an emulsifier and additives; Heating the mixture at 60° C. to 70° C. for 2 to 4 hours; And filtering the resultant product through a filter; The fig leaf alcohol extract has antioxidant, anti-inflammatory and whitening activities; The herbal medicine is made by finely powdering almonds, jullmu, yulpi, doin, baekgangjam, tosaja, mint, baekpyeon, white paper and mugwort; The persimmon extract is extracted from persimmon peel and persimmon, and is obtained by using a distillation method; The evening primrose seed oil saponified product is saponified by treating the evening primrose seed oil with an alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide, and potassium hydroxide; The sorghum powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 1 to 10% by weight of an alkali untreated and continuously pulverized for 48 to 72 hours; The ginseng extract refers to a substance in which a specific ginseng saponin substrate is increased by fermenting ginseng; The mung bean extract is obtained by immersing mung bean powder in an aqueous alcohol solution and contains bitexin and isobitexin as active ingredients. It relates to a method for preparing a cosmetic composition comprising a fig leaf alcohol extract.

Preferably, the fig leaf alcohol extract is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that it has antioxidant activity, anti-inflammatory activity and whitening activity.

More preferably, the antioxidant activity is a method for preparing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that the fig leaf alcohol extract is made of 5.0 to 46.7 mg/g polyphenol at a concentration of 0.1 to 1 mg/mL.

More preferably, the antioxidant activity is a method for preparing a cosmetic composition comprising a fig leaf alcohol extract characterized by an ABTS free radical scavenging activity of 3.8 to 5.2% at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract.

More preferably, the antioxidant activity is a method for producing a cosmetic composition comprising a fig leaf alcohol extract characterized by a DPPH free radical scavenging activity of 0.2 to 25.8 mg/g at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract.

More preferably, the anti-inflammatory activity is a fig, characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 ㎍ / ml of fig leaf alcohol extract. This is a method for preparing a composition containing leaf alcohol extract.

More preferably, the anti-inflammatory activity is a fig, characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 ㎍ / ml of fig leaf alcohol extract This is a method for preparing a composition containing leaf alcohol extract.

More preferably, the whitening activity comprises fig leaf alcohol extract, characterized in that the expression inhibitory activity of tyrosinase at a concentration of 0.265 to 2.5 mg/ml of fig leaf alcohol extract is 15.7 to 82.5%. This is a method of manufacturing a cosmetic composition.

As a means for solving the above problem, another embodiment according to the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, Fig leaf as any one formulation selected from the group consisting of hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, and loose powder. It is a method for producing a cosmetic composition containing alcohol extract.

The effect according to the present invention provides a manufacturing method capable of obtaining a cosmetic composition having a more enhanced cosmetic effect by containing various natural ingredients, centering on the antioxidant, anti-inflammatory and whitening activities of the fig leaf composition.

1 is a standard curve using gallic acid as the total polyphenol content of the fig leaf EtOH extract; B is the total polyphenol content (mg gallic acid quivalent/g sample) of fig leaves derived using the standard curve.
Figure 2 is about the ABTS free radical scavenging activity content of the fig leaf ethanol extract, A is a standard curve using ascorbic acid; B is the scavenging activity of ABTS radicals in fig leaves (mg ascorbic acid quivalent/g sample) derived using the standard curve.
Figure 3 relates to the DPPH free radical scavenging activity content of the fig leaf ethanol extract, where A is a standard curve using ascorbic acid and B is the DPPH radical scavenging activity of fig leaves derived using the standard curve (mg ascorbic acid quivalent/ g sample).
4 is a view of the toxicity evaluation of the fig leaf EtOH extract on RAW 264.7 cells, A is a sample concentration of 1 ~ 1,000 μg / mL; B is 10-50 μg/mL.
Figure 5 shows the activity of inhibiting nitric oxide production of the ethanol extract of fig leaf in RAW 264.7 cells induced by LPS.
Figure 6 shows the (A) IL-6 and (B) IL-12 production inhibitory activity of fig leaf EtOH extract in LPS-induced RAW 264.7 cells.
Figure 7 shows the effect of fig leaf inhibiting tyrosinase activity and inhibiting LDOPA oxidation reaction.
Figure 8 is a diagram confirming the cytotoxicity of a mixture of fig leaf EtOH extract and arbutin.
9 shows the inhibition of melanin synthesis by a mixture of fig leaf EtOH extract 0.5% and arbutin 0.5mg/ml.
10 shows the effect of inhibiting tyrosinase activity by a mixture of fig leaf EtOH extract 0.5% and arbutin 0.5mg/ml.
11 shows the effect of reducing the expression of tyrosinase mRNA by a mixture of 0.5% of fig leaf EtOH extract and 0.5mg/ml of arbutin.
FIG. 12 shows the effect of reducing the expression of tyrosinase protein by a mixture of 0.5% of fig leaf EtOH extract and 0.5mg/ml of arbutin.
Figure 13 shows the effect of inhibiting the activity of mushroom-derived tyrosinase by a mixture of fig leaf EtOH extract 0.5% and arbutin 0.5mg/ml.

Figs are a long-standing traditional food that does not use any pesticides, and is a completely pollution-free fruit. It has excellent food value due to its pharmaceutical effect to prevent adult diseases, and various antimicrobial activities such as ficine and minerals contained in figs. It is known that there are many substances.

Figs are also used as athlete's foot and anthelmintic repellent, and various active substances exist in fig leaves. However, there is insufficient research on extracting fig leaves or fruits, separating pharmacologically active substances, measuring the active effect on each active substance, and using it as a cosmetic composition.

Therefore, the inventors have come to invent a cosmetic composition including fig leaf alcohol extract, but in which conventionally known substances are mixed to enhance the cosmetic effect.

Hereinafter, specific examples and related descriptions of the invention are as follows.

According to an embodiment of the present invention, as a means for solving the above problem, an embodiment according to the present invention includes fig leaf alcohol/ethanol extract; Mixing herbal medicines, persimmon extract, evening primrose oil saponification, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract with an emulsifier and additives; Heating and stirring the mixture at 50° C. to 70° C. for 2 to 4 hours; And filtering the resultant product through a filter; The fig leaf alcohol extract has antioxidant, anti-inflammatory and whitening activities; The herbal medicine is made by finely powdering almonds, jullmu, yulpi, doin, baekgangjam, tosaja, mint, baekpyeon, white paper and mugwort; The persimmon extract is extracted from persimmon peel and persimmon, and is obtained by using a distillation method; The evening primrose seed oil saponified product is saponified by treating the evening primrose seed oil with an alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide, and potassium hydroxide; The sorghum powder extract is 0.5 to 5.0% by weight; The pork potato powder extract is 0.5 to 5.0% by weight; The cacao bean pulverized product is 1 to 10% by weight of an alkali untreated and continuously pulverized for 48 to 72 hours; The ginseng extract refers to a substance in which a specific ginseng saponin substrate is increased by fermenting ginseng; The mung bean extract is obtained by immersing mung bean powder in an aqueous alcohol solution, and contains bitexin and isobitexin as active ingredients. It provides a method for preparing a cosmetic composition comprising a fig leaf alcohol extract.

The herbal medicines are finely powdered almonds, adlay, yulpi, doin, baekgangjam, tosaja, peppermint, white grade, white paper and mugwort, and exhibit a whitening effect, as well as remove blemishes and itchiness, and feel moist and clean after use. To be there.

The herbal medicines listed above, that is, passersby are spots, freckles, and whitening. Skin cleanliness; Yulmu promotes skin metabolism, skin recovery, whitening, skin redox, melasma, warts; Doin has spots, freckles, moisturizing, whitening; White sensation removes spots, freckles, appearance, digestion, and itching; Tosa is blemishes, freckles, acne, blemishes, skin moisture; Peppermint is a boil, swelling, itching, skin disease, headache, neuralgia, migraine, toothache, promotion of blood circulation, fever; White paper pore contraction, skin moisture, whitening, itching removal; Wormwood is effective in cell regeneration, antibacterial, anti-inflammatory, melasma, freckles, and acne.

The fig leaf alcohol extract is most preferably extracted using edible ethanol. In the case of ethanol, it is easy to extract both hydrophilic and hydrophobic components, and it is advantageous that it does not generate harmful gases and is edible. Ethanol is about 40 to 80% pure ethanol. Preferably, about 70% ethanol (ethanol:water=7:3) is used.

Fig leaf alcohol extraction is performed by mixing the fig leaf crushed product and ethanol in a ratio of about 1:10 to 1:20 based on weight, and then extracting for 4 days using a low-temperature decompression method. In low-temperature decompression extraction, the object to be extracted is put into a vacuum extractor, heated to 50 to 80°C, and then the concentrator is reduced to -0.06 MPa to maintain a vacuum. After adjusting the temperature of the concentrator to about 40℃ while maintaining the vacuum, it is concentrated for 4 days under reduced pressure. When the operation of the extractor is started while the vacuum is applied in this way, the bubbles rise first, so the extraction is performed after adjusting the degree of decompression.

On the other hand, the herbal medicine used in the present invention is washed with purified water and dried, and then 100% by weight of ethanol is filled in the inside of an onggi jar, and then the dried herbal medicines listed above are immersed therein, and the top of the onggi jar is tightly sealed with the outside. Sealed pottery jars are stored in a warehouse out of sunlight for 2 to 5 days to sterilize the herbal medicines listed above, and when sterilized, remove the herbal medicines listed above from the pottery jar and filter them with gauze. The dried herbal medicines are finely powdered to 170-200 mesh using a separate grinder to obtain herbal medicines.

These micronized herbal medicines are preferably 3 to 5% by weight. If it is less than 3% by weight, it is difficult to expect a whitening effect and a removal effect such as blemishes and itchiness, and if it is more than 6% by weight, a whitening effect and removal effect such as blemishes and itchiness are excellent, but cleaning power is lowered and it is uneconomical. More preferably, it is 5% by weight.

The persimmon extract is extracted from persimmon peels and persimmons that have excellent skin whitening and antibacterial functions and has a skin exfoliating function, and is obtained by distillation. After pulverizing the persimmons and persimmons, the pulverized product is oiled. And mixed with caustic soda, washed, dried, and then distilled to obtain an extract.

The tannin and proanthocyanidin components contained in the persimmon bark and persimmon extracts are water-soluble substances and have antibacterial, antioxidant, anti-tumor and heavy metal removal effects, resulting in skin troubles due to excellent antibacterial and antioxidant activities. Can be prevented.

It is preferable to add 0.5 to 3% by weight of the extract obtained from the persimmon shell. When the pulverized persimmon shell is added in an amount of less than 0.5% by weight, the scrub effect of the soap is lowered, which is not preferable, and when it is added in excess of 3% by weight, odor may occur, which is not preferable.

The extract obtained from the persimmon chinensis is preferably 2 to 30% by weight. If it is added in less than 2% by weight, the whitening function of the extract is not preferable, and if it is added in more than 30% by weight, the color of the soap becomes excessively dark and a odor may occur, which is not preferable.

The evening primrose seed oil saponified product is saponified by treating the evening primrose seed oil with an alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide, and potassium hydroxide.

The evening primrose seed oil refers to an oil component obtained by physically pulverizing evening primrose seeds and then juiced, and the saponification is a reaction of hydrolyzing ester bonds in fats and oils contained in the evening primrose oil to produce carboxylic acid and alcohol. In other words, it means the reverse reaction of the esterification reaction, and the "saponifiable" refers to a fatty acid alkali salt hydrolyzed by treating the ester group bonds in the fat or oil component contained in the evening primrose oil with a "saponification reagent". Examples of the "saponification reagent" that can be used include alkali metal hydroxides such as sodium hydroxide, potassium hydroxide, or lithium hydroxide.

The evening primrose seed oil saponified product has an excellent skin whitening effect through inhibition of the production of melanin pigment, and is effective in treating and preventing skin pigmentation disorders caused by excessive production of melanin pigment.

The evening primrose seed oil saponified product is prepared by mixing evening primrose seed oil and a 2.5M KOH solution dissolved in ethanol at a volume ratio of 2:1 and heating to 80°C to prepare an evening primrose seed oil saponification product.

In order to confirm whether the evening primrose seed oil saponified product reduces the production of melanin pigment, melanoma cells are treated with Isobutylmethylxanthine (IBMX) to create an environment that promotes melanin production, and the evening primrose seed oil saponified product was compared with a control to reduce melanin production. It was confirmed to decrease.

Human melanoma cells were treated with IBMX at a concentration of 0.1 mM to promote the production of melanin pigment in melanoma cells, and saponified evening primrose oil was treated at a concentration of 0 to 100 ug/ml. Human melanoma cells treated with the evening primrose seed oil saponification product were cultured for 3 days.

In order to measure the melanin pigment content of the cultured melanoma cells, they were washed with physiological saline, dissolved in lysis buffer, and then treated with 20% TCA solution. After washing the precipitate twice with 10% TCA solution, it was again sequentially treated with a mixed solution of ethanol and diethyl ether (3:1), and a diethyl ether solution, dried in air, and then 0.85 M potassium hydroxide. It was dissolved into a solution and heated for 15 minutes. After cooling, the absorbance was measured at 440 nm. To correct the difference in melanin pigment content according to the number of cells, protein was quantified by the Bradford Method (Anal Biochem 1976;72:248-54), and the measured amount of melanin pigment was divided by the protein amount per 1 mg of protein. The measured amount of melanin pigment production (measured amount of melanin pigment/protein) is calculated.

As a result, the non-saponified evening primrose oil treatment control group did not significantly decrease the amount of melanin pigment produced even when the dose was increased to a concentration of 100 ug/ml.

On the other hand, in the group treated with the evening primrose seed oil saponified product, the amount of melanin pigment produced in cells was decreased depending on the treatment dose, and it was observed that melanin production was suppressed to about 90% at a concentration of 100.0ug/ml.

As a result of cell experiments as described above, it was found that the saponified evening primrose oil has an excellent effect in inhibiting the production of melanin pigments in actual cells.

The sorghum powder extract and the pork potato powder extract are 0.5 to 5.0% by weight, and phenol compounds, proanthocyanins, and vitamin B, which improve skin moisture, are extracted.

Using the sorghum powder extract and pork potato powder extract as raw materials, a cosmetic soap composition in the form of solid soap was prepared by extruding, cutting, and molding in a soap manufacturing machine, and skin irritation and skin moisturization were tested.

The skin irritation test was conducted in a series of evaluation criteria (0: no response to the skin; 1: partial skin pleura; 2: overall skin pleura; 3: skin pleura) and skin Is swollen; 4: skin pleura occurs and the skin is severely cracked), and the results are averaged.

In the above skin moisturizing experiment, since makeup residues and wastes changed the moisture level, it was measured with a moisture meter 2 hours after washing. Then, when the moisture meter is pressed against the skin area to be measured, the moisture content, that is, the skin moisture level, is checked on the LCD panel that detects the electronic action. The display unit is %, and if the moisture is less than 37%, it is very dry, and the average is 37 to 47%. The judgment was conducted on 20 subjects, and the results were averaged.

As a result of checking the degree of skin irritation, it was found that there was no reaction to the skin, or skin thorax occurred partially. And, as a result of checking the skin moisture level with a moisture meter after 2 hours after washing, it was checked as 55 to 78%, which is higher than the skin moisture level of 37 to 47% of a normal person.

The cacao bean pulverized product is 1 to 10% by weight of continuous pulverization for 48 to 72 hours without alkali treatment, and the cacao bean contains a large amount of polyphenol, which is an anti-aging and antioxidant component, and 72 to 72 hours by a crusher. It is to be pulverized continuously until time, but immediately after roasting the raw cacao beans at 200°C for 20 to 30 minutes, the cacao beans are shelled, and then pulverized immediately without mixing with an alkali agent to be used.

When making foods such as chocolate using cacao beans, cacao beans are used by mixing with an alkali agent to remove the skin and neutralize taste such as acidity immediately after roasting, but in the present invention, cacao beans are pulverized immediately without mixing an alkali agent. Maximize the polyphenol content of soybeans.

The polyphenol content was tested by varying the roasting temperature and time.

Total polyphenols from Sample 1 (Roasted at 150°C for 20 minutes), Sample 2 (Roasted at 150°C for 30 minutes), Sample 3 (Roasted at 200°C for 25 minutes), and Sample 4 (Roasted at 200°C for 30 minutes) The contents were 14.5mgGAE/g, 15.3mgGAE/g, 16.8mgGAE/g, and 15.9mgGAE/g, respectively. Therefore, the total polyphenol content of cacao beans roasted at 200°C for 25 minutes was the highest.

The cacao bean pulverized product is 1 to 10% by weight, preferably, it is sufficient to add in the range of 5 to 8% by weight. If the content is out of these contents, it is difficult to obtain the effect intended in the present invention, and if the content of each component is exceeded, it is difficult to obtain the desired effect, and there is a concern that it may adversely affect the skin by irritating the skin. Is insufficient, so the desired result cannot be obtained.

The ginseng extract refers to an increase in a specific ginseng saponin substrate by fermenting ginseng, thereby suppressing skin aging and improving wrinkles.

The fermented ginseng extract is characterized in that it is mixed in an amount of 0.01 to 50% by weight.

The fermented ginseng extract is prepared by mixing and culturing ginseng-derived bacteria active in beta-glucosidase, malt, bran and water to ferment and extract ginseng.Food, pharmaceutical and cosmetic compositions are used to inhibit skin aging. It can be formulated into foods, medicines and cosmetics for skin moisturizing, skin wrinkle improvement, skin whitening and pore reduction, sebum reduction, and skin moisturizing.

The fermented ginseng extract was prepared by culturing the bacteria isolated from yeast in a medium in which potato dextrose broth (PDB) and corn meal broth were mixed. Prepared ginseng was prepared by mixing with rice flour, typically grains, and then put into a fermentation tank. 100g of prepared rice flour, 100g of ginseng, 100g of water, and 50g of strain suspension were mixed and put in a fermenter, and fermented at a temperature of 30°C and 70% moisture to obtain an extract.

And as a control, an extract was obtained using ginseng without any treatment.

Sensory evaluation was performed using the fermented ginseng extract and the pill made using the control. 20 healthy women and men from 20 to 50 years of age were to take pills made with fermented ginseng 3 times a day for 2 weeks. After 2 weeks, the degree of skin aging inhibition and the degree of wrinkle improvement were evaluated through a questionnaire survey of subjects. Subjects judged that there were no improvement, slight improvement, and significant improvement in three stages. As a result, for the inhibition of skin aging, considerably improved (9 persons), slight improvement (7 persons), no improvement (4 persons); Regarding the degree of skin wrinkle improvement, it was counted as significantly improved (10 persons), slightly improved (8 persons), and no improvement (2 persons), and it was confirmed that the effect of suppressing skin aging and improving wrinkles when the fermented ginseng extract was taken.

The mung bean extract is obtained by immersing mung bean powder in an alcoholic aqueous solution, and contains bitexin and isobitaxin as active ingredients, and has excellent antioxidant activity, so it is effective in removing skin blemishes such as spots and freckles, and improving wrinkles.

The mung bean powder is prepared by first washing mung beans with clean water, removing impurities, and drying them, and sufficiently crushing the dried mung beans.

Thereafter, the crushed green beans were weighed to 1 kg, and then precipitated in an aqueous solution in which alcohol and water were mixed at a ratio of 7:3 and left for 5 days. Preferably the alcohol is ethanol.

The left sediment was filtered using a 300 mesh filter cloth, and the filtrate was concentrated at 70° C. using a vacuum concentrator to obtain a mung bean extract.

In order to examine whether the concentrated mung bean extract has an antioxidant effect, that is, removing blemishes such as spots and freckles, and improving wrinkles, as a control group, a mung bean extract from which bitexin and isobitexin are removed was separately prepared, and the concentrated mung bean extract And sensory evaluation was performed using a mask pack made using the control group. A mask pack made using the concentrated mung bean extract and the control group was used for 2 weeks once a day for 20 healthy women and men from 20 to 50 years old. After 2 weeks, the effects of removing blemishes such as spots and freckles and improving wrinkles were evaluated through a questionnaire survey of subjects. The test subject judged that there were no improvement, slight improvement, and significant improvement in three stages.As a result, the effect of removing skin blemishes from the concentrated mung bean extract was significantly improved (10 persons), slight improvement (8 persons), no improvement. (2 people); Regarding the degree of skin wrinkle improvement, it was counted as significantly improved (11 people), slightly improved (5 people), and no improvement (4 people), and antioxidant activity on the concentrated mung bean extract, that is, removing blemishes such as spots and freckles, and wrinkle improvement It was confirmed that there is an effect such as.

The fig leaf alcohol extract has antioxidant, anti-inflammatory and whitening activities.

The antioxidant activity is due to polyphenols of fig leaf alcohol extract, preferably 5.0 to 46.7 mg/g of polyphenol at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract.

There are over thousands of types of polyphenols, and in general, polyphenols are well known to prevent aging because they have antioxidant effects that can remove free radicals from our body. In addition, it has been reported to lower the risk of various diseases due to its excellent function of protecting DNA, cell-forming proteins and enzymes damaged by exposure to free radicals. It is also known to prevent heart disease with anticancer action.

In order to analyze the content of polyphenols contained in the fig leaf alcohol extract, the present inventors developed a color from blue to yellow when the Folin-Ciocalteus reagent was reduced by the polyphenol compound of the sample under alkaline conditions. It was measured using the principle of being.

_{That is, 2 mL of 2% Na 2} CO ₃ was added to 100 μL of the sample to form an alkaline condition, followed by reaction for 3 minutes, and 100 μL of 50% Pauline-Ciocalteus reagent was added to react for 30 minutes, and then 750 nm The total polyphenol content, which is an antioxidant component, can be confirmed by measuring at. A calibration curve was prepared using gallic acid as a standard material for quantification, and the total content of polyphenol compounds in the sample was equivalent to milligrams of gallic acid (mg gallic acid equivalents (GE)/g). Indicated.

As a result, the content of the total polyphenol compound of the fig leaf alcohol extract was measured in the concentration range of 0.1 to 1 mg/mL, showing a concentration-dependent trend and a content corresponding to 5.0 to 46.7 mg/g (Fig. One). This was confirmed to be a total polyphenol content of about 4.7 to 5.0% when converted to an amount corresponding to the gallic acid used as a standard material.

In addition, the antioxidant activity has a scavenging activity of ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) free radicals of fig leaf alcohol extract, but preferably at a concentration of 0.1 to 1 mg/mL It is characterized by having an ABTS free radical scavenging activity of 3.8 to 5.2% at.

In addition, the antioxidant activity is characterized by a DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity of 0.2 to 25.8 mg/g at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract.

DPPH and ABTS, which are chemically stabilized free radicals, give electrons when reacting with antioxidants, and the radicals disappear and change color.Thus, DPPH and ABTS are applied to the experiment by three types of fig leaf solvent-based fractions. The degree of lightening of the color was measured to indicate the antioxidant power.

To measure antioxidant activity using ABTS radicals, 7.4 mM of ABTS solution and 2.6 mM of oxidized _{potassium disulfate (K 2} S ₂ O ₈ , potassium persulphate) solution were left in the dark for more than 12 hours to form blue-green ABTS radicals. , Was used after diluting so that the absorbance value was 1.5 at 734 nm. Thereafter, 50 μL of a sample was added to the solution and allowed to stand at room temperature for 60 minutes to measure the change in absorbance value of the reaction solution. capacity, AEAC).

For the measurement of antioxidant activity using DPPH free radicals, 50 μL of a sample was added to a 0.2 mM DPPH radical solution and reacted at room temperature for 60 minutes to measure the change in absorbance of the reaction solution at 517 nm. The results were shown by comparing and calculating ascorbic acid equivalent antioxidant capacity (AEAC) to ascorbic acid, which is a standard substance, similar to ABTS scavenging activity.

As shown in FIG. 2, the ABTS free radical scavenging activity of fig leaf alcohol extract was measured in a concentration range of 0.1 to 1 mg/mL, showing a concentration-dependent trend, and it was found to have an antioxidant power of about 3.8 to 5.2%. This corresponds to an antioxidant power of 38-52 mg/g when converted to an amount equivalent to ascorbic acid used as a standard material.

Meanwhile, as shown in FIG. 3, in the antioxidant power using DPPH, which is another kind of radical, the activity of the fig leaf alcohol extract showed a concentration-dependent tendency in the concentration range of 0.1 to 1 mg/mL. When converted to the amount equivalent to the ascorbic acid used as a standard substance, fig leaf alcohol extract was found to have an antioxidant activity of 0.2-25.8 mg/g.

The results showed the best DPPH free radical scavenging activity in the EtOAc layer, similar to the ABTS free radical scavenging activity (107.1 mg ascorbic acid equivalent/g; about 10.7% antioxidant power compared to ascorbic acid), the H2O layer and the BuOH layer. Ascorbic acid showed 37.7 mg/g (3.8%) and 30.9 mg/g (3.1%) antioxidant power, respectively.

From the polyphenol content and the results of the two types of radical scavenging activity, the fig leaf alcohol extract can be estimated to have good results in the anti-inflammatory and whitening activity to be carried out later.

The anti-inflammatory activity is characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 μg/ml of fig leaf alcohol extract.

In addition, the anti-inflammatory activity is characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 ㎍ / ml of fig leaf alcohol extract.

To evaluate the anti-inflammatory activity, toxicity evaluation was performed on the RAW 264.7 cell line, which is a murine macrophage cell line.

The cell line was pre-sold from the Korean Cell Line Bank, and DMEM supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (P/S) In a medium (Dulbecco's modified Eagle's medium), it was subcultured once every 2-3 days under conditions of 37°C and 5% CO2 and used in the experiment.

First, in order to evaluate the toxicity of the sample to the cells, the cells were plated on a 96-well plate at 2×10 6 /well and incubated for 12 hours in an incubator at _{37°C and 5% CO 2 to stabilize the cells.} Subsequently, the culture medium was removed, and 160 µl of fresh medium and 20 µl of an appropriately diluted sample were added and incubated for 30 minutes. After administration of lipopolysaccharide from Escherichia coli (LPS) as an inflammatory substance, 24 It was re-cultivated over time. After removing all of the culture medium, 100 µl of EZ-cytox (Daeil Labservice) solution dissolved in PBS at a concentration of 10% was added to measure the absorbance at 450 nm to determine the cytotoxicity of the sample to the saline control group. It was expressed in terms of cell viability (%).

The inflammatory reaction in the body is a defense function against harmful stimuli such as microbial infection, endotoxin, and tissue damage, and this is a reaction that occurs essentially to restore normal tissue structure and function. The normal inflammatory response has a regulatory process in which the inflammatory response is limited by itself as the production of pro-inflammatory mediators decreases and anti-inflammatory mediators increase over time. Macrophage, one of the cells involved in the inflammatory response in the body, plays an important role in the inflammatory response by being activated by exposure to stimulation such as LPS, which is a cell membrane component of Gram-negative bacteria, and activated macrophages are interferon-γ (IFN-γ), It is known to promote the production of inflammatory cytokines such as IL-1β, IL-6, IL-12 and TNF-α.

In addition, through the expression of enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX) in addition to pro-inflammatory cytokines, inflammation mediating molecules such as nitric oxide (NO) and prostaglandin E2 (PGE2) are generated. It is known that excessive production contributes to the onset of various chronic inflammatory diseases. Research using RAW 264.7, a macrophage cell line, has been actively conducted in order to examine the availability as a health functional material capable of preventing or delaying the onset of inflammatory diseases. Examine the inflammatory activity.

Prior to the experiment, the toxicity of the sample to RAW 264.7 cells was evaluated. As shown in Figure 4A, the fig leaf ethanol extract did not show cytotoxicity in the concentration range of 1 to 100 μg/mL, but when the concentration was increased to 1,000 μg/mL, it showed about 55% cytotoxicity compared to the LPS-treated group. Confirmed.

Therefore, in the present invention, a concentration range of 10 to 50 μg/mL was selected as a sample concentration range in which cytotoxicity did not appear through retesting, and the anti-inflammatory activity was subsequently evaluated in the concentration range in which toxicity did not appear as shown in FIG. 4B.

As shown in Figure 5, the fig leaf alcohol extract did not show the inhibitory activity of LPS-induced nitric oxide (NO) in the range of 10 to 50 μg/mL, which is a concentration where cytotoxicity did not appear (p<0.05 ).

In measuring the inhibitory ability of inflammatory cytokines IL-6 and IL-12, fig leaf alcohol extract showed a concentration-dependent inhibitory activity effect, and this trend was statistically significant (FIG. 6).

In the case of IL-6 inhibitory activity (FIG. 6A), at a concentration of 10 to 50 μg/mL, the inhibitory activity was about 25.3 to 32.5% compared to the LPS treatment group, and all of these showed p<0.05 ( 95% confidence level).

The IL-12 inhibitory activity (Fig. 6B) also showed a concentration-dependent inhibitory effect at a concentration of 10 to 50 μg/mL, which was found to have an inhibitory ability of about 26.1 to 35.4% compared to the LPS treatment group. All were found to be statistically significant in the range of p<0.01 (99% confidence level) compared to the LPS treatment group.

The whitening activity is characterized in that it inhibits the expression of tyrosinase, and preferably, the activity of inhibiting the expression of tyrosinase at a concentration of 0.1 to 0.5% of fig leaf alcohol extract is 21.4 to 60.5%. It is characterized.

The tyrosinase inhibitory activity was tested in accordance with the Ministry of Food and Drug Safety's [Guideline for Efficacy Evaluation of Functional Cosmetics I], and the numerical value was one-way ANOVA- using Turkey's method (GraphPad Software, Inc., USA). Ordinary analysis was performed, and statistical significance was determined when the P value was less than 0.05.

The inhibition rate of tyrosinase activity and inhibition rate of L-DOPA oxidation reaction by the ethanol extract sample of fig leaf were measured as follows (FIG. 7). For comparison, the inhibition rate of arbutin 0.5mg/ml was investigated and shown.

Fig leaf ethanol extract (%) 0 0.1 0.25 0.5 Arbutin
0.5mg/ml Tyrosinase
Active average (%) 100.0 60.5 30.1 21.4 29.1 Standard Deviation 0.0 1.4 1.0 1.2 1.3

In the case of a sample of 0.5% ethanol extract of fig leaf, the inhibitory effect was 7.7%p higher than that of arbutin 0.5mg/ml.

Fig leaf ethanol extract (%) 0 0.1 0.25 0.5 Arbutin
0.5mg/ml L-DOPA oxidation reaction
Active average (%) 100.0 68.5 53.4 36.0 71.7 Standard Deviation 0.0 4.2 3.1 4.6 3.0

In the case of a sample of 0.5% ethanol extract of fig leaf, the inhibitory effect was 35.7%p higher than that of arbutin 0.5mg/ml.

8 to 13 show the effect of a mixture of fig leaf ethanol extract and arbutin 0.5mg/ml.

First, FIG. 8 shows the cytotoxicity of a mixture of fig leaf ethanol extract and arbutin 0.5 mg/ml according to the concentration of fig leaf ethanol extract, and 90 when treated with arbutin 0.5 mg/ml and fig leaf ethanol extract (A Extract) 0.5%. Since the cell viability was shown in %, the efficacy test was conducted at a concentration below that.

9, in the case of a mixture of 0.5mg/ml of arbutin and 0.5% of fig leaf ethanol extract (A Extract), it was found that the inhibitory effect of melanin synthesis was improved by about 31%.

According to FIG. 10, in the case of a mixture of 0.5mg/ml of arbutin and 0.5% of fig leaf ethanol extract (A Extract), the inhibitory effect of tyrosinase activity was improved by about 13%.

According to FIG. 11, in the case of a mixture of 0.5mg/ml of arbutin and 0.5% of fig leaf ethanol extract (A Extract), the effect of reducing tyrosinase mRNA expression was improved by about 47%.

According to FIG. 12, in the case of a mixture of 0.5 mg/ml of arbutin and 0.5% of fig leaf ethanol extract (A Extract), the effect of reducing tyrosinase protein expression was improved by about 55%.

13, in the case of a mixture of 0.5mg/ml of arbutin and 0.5% of fig leaf ethanol extract (A Extract), the inhibitory effect of mushroom-derived tyrosinase activity was improved by about 65%.

In the cosmetic composition of the present invention, in addition to the fig leaf alcohol extract, which is an essential component, other ingredients that are usually blended in cosmetics can be blended as needed, and such blending ingredients include fats and oils, moisturizers, emollients, surfactants, and emulsifiers. , Organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, limiting agents, and purified water.

In addition, according to an embodiment of the present invention, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence , Pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, and any one formulation selected from the group consisting of loose powder to obtain a cosmetic composition containing fig leaf alcohol extract I can.

According to an embodiment of the present invention, after diluting the fig leaf alcohol extract in purified water at a ratio of about 0.01 to 0.25%, mixing other ingredients and an emulsifier, etc., and dispersing and emulsifying a cosmetic composition in a cream form can be prepared. have.

Into a cream formulation, other additional active ingredients and/or ingredients that can be conventionally mixed may be mixed to prepare a cosmetic composition.

On the other hand, in the cosmetic composition comprising the fig leaf alcohol extract according to the present invention; A soap prepared by further comprising herbal medicines, persimmon extract, evening primrose oil saponified product, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract, and the combination of each component It was confirmed that it has a higher synergistic effect than the simple sum.

Specifically, each component of fig leaf alcohol extract, herbal medicine (all those listed as herbal medicines above), persimmon extract, evening primrose seed oil saponified product, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract Soap (each control group) and soap (experimental group) in which all of the above ingredients were combined were prepared, and this was prepared for 9 groups with 20 adults in their 20s to 50s as a group, and 9 control groups and experimental groups. Distributed to each one group and repeated washing three times a day for one week, a questionnaire survey was conducted on the changes over two weeks.

More specifically, the present invention, the fig leaf alcohol extract is 5 to 15% by weight, preferably 10% by weight, the herbal medicine is 10 to 20% by weight, preferably 15% by weight, the persimmon extract is 5 to 10% by weight %, preferably 7% by weight, the evening primrose seed oil saponified product is 5 to 15% by weight, preferably 10% by weight, the sorghum powder extract is 0.5 to 5.0% by weight, preferably 2.5% by weight, the pork potato powder The extract is 0.5 to 5.0% by weight, preferably 2.5% by weight, and the pulverized cacao bean is 1 to 10% by weight. Preferably, 5% by weight, and those containing bitexin and isobitexin as the mung bean extract are 8 to 12% by weight, preferably 10% by weight.

Therefore, the experimental group is the fig leaf alcohol extract 10% by weight, the herbal medicine 15% by weight, the persimmon extract 7% by weight, the evening primrose seed oil saponified product 10% by weight, the sorghum powder extract 2.5% by weight, the pig potato powder extract 2.5% by weight %, 5% by weight of the crushed cacao bean, and 10% by weight of the mung bean extract were prepared from soaps containing soap paste and the like.

On the other hand, the control group has the same weight% as the experimental group, but the rest is used as a soap prepared by using a soap paste or the like.

As a result, as a result of the questionnaire survey for the first group that used the experiment group with soap (control group) made with only fig leaf alcohol extract as a single raw material, significantly improved (10 people), slight improvement in the experimental group compared to the control group. It was counted as (6 people) and no change (4 people).

As a result of a questionnaire survey on the second group that used the experimental group and soap made with only herbal medicines (all those listed as herbal medicines above) as a single raw material, significantly improved (9 persons) in the experimental group compared to the control group, slightly Was counted as improvement (5 people) and no change (4 people).

As a result of a questionnaire survey on the soap (control group) made with only persimmon extract as a single raw material and the third group to use the experimental group, significantly improved (7 people), slight improvement (5 people) in the experimental group compared to the control group, It was counted as no change (8 people).

As a result of a questionnaire survey for the fourth group that used the experimental group and the soap (control group) made with only evening primrose seed oil saponified product, compared to the control group, there was a significant improvement (7 people) and a slight improvement (5 people). ), no change (8 people).

As a result of a questionnaire survey of soap (control group) made with only sorghum powder extract as a single raw material and the 5th group that used the experiment group, significantly improved (6 people), slight improvement (6 people) in the experimental group compared to the control group , It was counted as no change (8 people).

As a result of a questionnaire survey for the 6th group that made use of the soap (control group) and the experimental group, which was made with only pork potato powder extract, significantly improved (5 people), slight improvement (5 people) in the experimental group compared to the control group. ), no change (10 people).

As a result of a questionnaire survey on soap (control group) made with only cacao bean crushed product as a single raw material, and the 7th group that used the experiment group, significantly improved (6 people), slight improvement (3 people) in the experimental group compared to the control group. ), no change (11 persons).

As a result of a questionnaire survey of soap (control group) made with only ginseng extract as a single raw material and the 8th group that used the experiment group, significantly improved (6 people), slight improvement (5 people) in the experimental group compared to the control group. , It was counted as no change (11 persons).

As a result of a questionnaire survey on soap (control group) made with only mung bean extract as a single raw material and the 9th group that used the experiment group, significantly improved (7 people), slight improvement (7 people) in the experimental group compared to the control group, It was counted as no change (6 persons).

As a result of this survey, in the cosmetic composition comprising the fig leaf alcohol extract; Herbal medicine, persimmon extract, evening primrose seed oil saponified product, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract further included are significantly improved compared to those used alone as one of these ingredients. Confirmed that there is.

Claims (8)

As a method for producing a cosmetic composition comprising a fig leaf alcohol extract;
Fig leaf alcohol extract; Mixing herbal medicines, persimmon extract, evening primrose oil saponification, sorghum powder extract, pork potato powder extract, cacao bean crushed product, ginseng extract and mung bean extract with an emulsifier and additives;
Heating and stirring the mixture at 60°C to 70°C for 2 to 4 hours; And
As a method for producing a cosmetic composition comprising the step of filtering the resultant product through a filter;
The fig leaf alcohol extract has antioxidant, anti-inflammatory and whitening activities;
The herbal medicine is made by finely powdering almonds, jullmu, yulpi, doin, baekgangjam, tosaja, peppermint, baekpyeon, white paper and mugwort;
The persimmon extract is extracted from persimmon peel and persimmon, and is obtained by using a distillation method;
The evening primrose seed oil saponified product is saponified by treating the evening primrose seed oil with an alkali metal hydroxide selected from the group consisting of sodium hydroxide, lithium hydroxide, and potassium hydroxide;
The sorghum powder extract is 0.5 to 5.0% by weight based on the total weight of the cosmetic composition;
The pork potato powder extract is 0.5 to 5.0% by weight based on the total weight of the cosmetic composition; The cacao bean pulverized product is 1 to 10% by weight obtained by continuous pulverization for 48 to 72 hours without alkali treatment, wherein the weight% is based on the total weight of the cosmetic composition;
The ginseng extract means that ginseng is fermented to increase a specific ginseng saponin substrate;
The mung bean extract is obtained by immersing mung bean powder in an aqueous alcohol solution, and contains bitexin and isobitexin as active ingredients;
A method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that. The method of claim 1,
The fig leaf alcohol extract is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that it has antioxidant activity, anti-inflammatory activity and whitening activity. The method of claim 2,
The antioxidant activity is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that the fig leaf alcohol extract at a concentration of 0.1 to 1 mg/mL and 5.0 to 46.7 mg/g of polyphenol. The method of claim 2,
The antioxidant activity is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized by the ABTS free radical scavenging activity of 3.8 to 5.2% at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract. The method of claim 2,
The antioxidant activity is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized by a DPPH free radical scavenging activity of 0.2 to 25.8 mg/g at a concentration of 0.1 to 1 mg/mL of fig leaf alcohol extract. The method of claim 2,
The anti-inflammatory activity includes fig leaf alcohol extract, characterized in that the IL-6 inhibitory activity is 25.3 to 32.5% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 μg/ml of fig leaf alcohol extract. A method for producing a cosmetic composition. The method of claim 2,
The anti-inflammatory activity includes fig leaf alcohol extract, characterized in that the IL-12 inhibitory activity is 26.1 to 35.4% compared to the anti-inflammatory activity (inflammatory cytokine) LPS at a concentration of 10 to 50 μg/ml of fig leaf alcohol extract. A method for producing a cosmetic composition. The method of claim 2,
The whitening activity is a method for producing a cosmetic composition comprising a fig leaf alcohol extract, characterized in that the expression inhibitory activity of tyrosinase (tyrosinase) is 21.4 to 60.5% at a concentration of 0.1 to 0.5% of fig leaf alcohol extract.

Images