KR101189794B1 - Composition for external application to the skin containing Hibiscus cannabinus extract - Google Patents

Abstract

The present invention is a sheep ( Hibiscus) The present invention relates to an external composition for skin containing cannabinus ) extract, and more particularly, by containing a yam extract, in particular a yam extract, a yam extract or a bark extract of a yam stem, an anti-aging skin having an excellent effect of promoting skin cell and collagen biosynthesis. It relates to an external preparation composition.

Description

Composition for external application to the skin containing Hibiscus extract {Composition for external application to the skin containing Hibiscus cannabinus extract}

The present invention relates to a composition for external application for skin containing a yam extract, and more specifically, to aging the skin cells and collagen biosynthesis excellent by containing the extract extracted after drying the yam leaf, yam branch or yam stem shell as an active ingredient It relates to an external composition for skin having a prophylactic effect.

Hibiscus cannabinus is a year-old herbaceous plant belonging to the Rose of Sharon, whose main cultivation areas are West and Southern Africa, East India, Cuba, Thailand, Australia, and India and Thailand in Asia. It was the first time in Korea that it was introduced as a test cultivation of host plants of cotton leg moths, not for textiles. In 1926, there was some cultivation before 1945, but there is currently no cultivation area for fiber production. It is similar in quality and value to the use of jute fibers and juveniles, and its color is good but rough and strong. In addition, the horse is fast growing and rich in fiber, making it suitable as a raw material for paper, various adsorbents and filtration materials, and attracting attention as a plant that can simultaneously solve global environmental problems such as global warming and deforestation due to reduced carbon dioxide emissions. The main components of horses contain excessive amounts of tannins, saponins, polyphenols and alkaloid compounds, and are known to be used in folk remedies in India and Africa for sore throat disorders, bruises and bile control. Recent studies have shown that hemp (kenaf) extracts have immunomodulatory and antimicrobial effects. However, there have been no reports of anti-aging effects on skin aging, and the effects of other parts of horses other than leaves have been studied. There is no situation.

Skin is the body's primary protective film that protects organs in the body from changes in temperature and humidity, irritation from the external environment such as UV and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, excessive physical and chemical stimulation and stress malnutrition from the outside will degrade the normal function of the skin and promote skin aging such as loss of elasticity, keratinization and wrinkle formation. In order to maintain the conventional use of cosmetics reinforced with bioactive substances obtained from various plants, microorganisms, etc., efforts have been made to effectively maintain skin's inherent functions and to effectively inhibit skin aging by activating skin cells. And conventional cosmetic raw materials are mostly ineffective, or cause various problems such as causing skin side effects. Therefore, there is a need for research on raw materials having an anti-aging effect without causing skin side effects.

Korean Patent Publication No. 2007-0111409 discloses a kenaf extract, a novel camperol compound isolated therefrom, and a use thereof as a food thereof, and Korean Patent No. 0840558 discloses a kenaf extract having anti-inflammatory and immunomodulatory activity. Although it is disclosed, it is different from the external composition for skin aging for preventing skin aging containing the sheep extract of the present invention as an active ingredient.

The present invention is derived from the above requirements, in the present invention, by extracting the bark of the leaf of the sheep, the stem of the sheep and the stem of the stem as an active ingredient excellent skin cell and collagen production promoting effect is excellent skin external composition for preventing skin aging The present invention has been completed by the development.

In order to solve the above problems, the present invention provides a skin external preparation composition for preventing skin aging containing the extract of the bark of the sheep, leaf and the stem of the sheep using a solvent as an active ingredient.

In addition, the present invention provides a cosmetic composition for preventing skin aging or improving wrinkles containing a sheep extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing skin aging or improving wrinkles, which contains a sheep extract as an active ingredient.

In addition, the present invention provides a health food for skin aging or anti-wrinkle containing a sheep extract as an active ingredient.

According to the present invention, the skin external preparation composition containing the yam extract has an excellent effect on promoting skin cell and collagen biosynthesis, inhibits skin aging, and is effective for anti-aging and wrinkle improvement, and because it contains physiologically active substances obtained from plants. It can provide an external preparation for skin that is excellent and does not cause side effects.

In addition, by first extracting the horses in a polar solvent and then re-extracted with a low polar solvent, the extracts are sequentially extracted by changing the polarity to maximize the active ingredient content and remove impurities to improve the stability and at the same time maximize the efficacy It can be added to external skin preparations such as softening water using solubilization technology, nutrient cosmetic water applying cream technology, cream, essence and the like.

In order to achieve the object of the present invention, the present invention provides a composition for external application to the skin containing a yam extract as an active ingredient.

The sheep (kenaf) extract has an anti-aging or anti-wrinkle effect, and the anti-aging or anti-wrinkle effect may be a skin cell proliferation effect or collagen biosynthesis promoting effect.

The yam extract of the present invention may preferably be extracted from the bark of the yam leaf, the yam branch (part between the leaves and the stem) or the yam stem.

The yam extract

(a) drying and pulverizing the sheath leaf, the yam branch or the yam stem shell;

(b) extracting the pulverized product by cooling or warming with an extraction solvent; And

(c) The extract may be prepared by concentrating under reduced pressure.

The extraction step of (b) may be first extracted using a polar solvent and then reextracted using a low polar organic solvent. The polar solvent may be water, anhydrous or hydrous lower alcohol (methanol, ethanol, etc.), acetone, It may be one or a mixture of 1,3-butylene glycol, normal propanol, isopropanol and normal butanol, but is not limited thereto. In addition, the low polar organic solvent may be any one or a mixture of ethyl acetate, diethyl acetate, chloroform, diethyl ether, benzene, and hexane, but is not limited thereto.

Referring to the method for producing a sheep extract of the present invention in more detail as follows. Finely grind the leaves of the hemp (kenaf), the eggplant (the part between the leaves and the stem) or the bark of the stem, and water, lower alcohol (methanol, ethanol), Polar solvents such as a mixture of water and lower alcohol, acetone, 1,3-butylene glycol, normal propanol, isopropanol, and normal butanol, and mixed solvents thereof were added by 5-20 times by volume, followed by immersion extraction and filtration. Concentrate under reduced pressure. Water is added to the concentrated solution to disperse the same, and then the same amount of low polar organic solvent such as ethyl acetate, chloroform and diethyl ether is added to the dispersion and shaken. The organic layer is separated and concentrated under reduced pressure to obtain an excellent extract. Extraction method is about 12-96 hours for cold acupuncture, or it is left to mature for 3-20 days at room temperature of 4-25 ℃ using anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or diethyl ether as a solvent. You can also extract In the case of a warm needle, it is different depending on the type and temperature of the solvent and the like to be used, but it is preferable to perform about 5-24 hours at a temperature close to the reflux temperature of the solvent.

The external preparation composition for skin containing the extract of horseshoe according to the present invention preferably contains 0.0001 to 10% by weight of the sheepskins extract based on the total weight of the composition, but is not limited thereto.

The present invention also provides a cosmetic composition for preventing skin aging or improving wrinkles, containing a sheep extract as an active ingredient. The cosmetic composition of the present invention is softening cosmetics, astringent makeup, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil And it may have a formulation selected from the group consisting of essences, but is not limited thereto.

In addition, the cosmetics, in addition to the sheep extract of the present invention, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic field, such as any other ingredient.

The present invention also provides a pharmaceutical composition for preventing skin aging or improving wrinkles, which contains a sheep extract as an active ingredient.

The external preparation composition for skin according to the present invention may be a pharmaceutical composition, in which case, the extract of horseskins may be used in medicine as it is or in the form of a pharmaceutically acceptable salt. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , Benzene sulfonic acid, toluene sulfonic acid, naphthalene sulfonic acid and the like can be used. In addition to acid addition salts, base addition salts such as sodium hydroxide, potassium hydroxide, triethylamine, tert-butylamine can also be used.

In the external preparation composition for skin according to the present invention, in the pharmaceutical formulation, the content of the yam extract may vary widely depending on the formulation, and according to a conventional method.

The external preparation for skin pharmaceutical composition according to the present invention may be formulated by adding various auxiliaries used in medicine, such as a carrier or other additives such as stabilizers, emollients, emulsifiers, etc. as necessary, so long as they do not adversely affect the active ingredient. .

In addition, the composition containing the horseshoe extract according to the present invention can be administered parenterally, orally. As a parenteral route, transdermal administration is preferred, and topical application is most preferred. The formulation may be any formulation suitable for pharmaceutical preparations, including ointments, creams, injections, powders, granules, tablets and the like.

Preferred dosages of the pharmaceutical compositions according to the invention are 0.001 to 1000 mg / Kg? Day.

The compositions according to the invention can be administered alone or in combination with other agents or together to assist other agents.

In addition, in the external preparation composition of each formulation, other components other than the above-mentioned essential ingredient of the horsetail extract can be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use of other external preparation, in which case when applied simultaneously with other raw materials Synergistic effects may occur.

The present invention also provides a health food for anti-aging or wrinkle improvement containing the extract of sheep as an active ingredient.

When the sheep's horse extract of the present invention is used as a food additive, the sheep's horse extract can be added as it is or used with other food or food ingredients, and can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, in the preparation of food or beverages, the composition of the present invention is added in an amount of up to 15 parts by weight, preferably up to 10 parts by weight based on the raw materials. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the yam extract of the present invention includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, And carbonating agents used in carbonated drinks. In addition, the yam extract of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the configuration and effect of the present invention will be described in more detail with reference to Examples and Experimental Examples. However, the present invention is not limited to these examples.

Example  One: Sheep  Preparation of Leaf Extract

After washing the horses (kenaf) leaves with purified water and drying, 100 g of the finely ground horseshoe (kenaf) leaf powder was put in 1 L of 50% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with 300 mesh filter cloth. The filtrate was left to mature at 4-25 ° C. for 7 days and then filtered through Whatman No. 2 filter paper. Add the extract to a 3 L separatory funnel, add 1 L of ethyl acetate, shake well, mix well, separate, and completely separate into two layers, take the upper layer (ethyl acetate layer), and place the lower layer (water layer) again with the separatory funnel. Extracted once more. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 15.1 g.

Example  2: Sheep  Preparation of Eggplant Extract

Wash the eggplant (the part between the leaves and the stem) of the sheep (kenaf) with purified water, dry it, add 100 g of the finely ground sheep eggplant powder to 1 L of 50% ethanol aqueous solution, boil it for 12 hours in an extractor with a cooling condenser, and extract 300 The filtrate was filtered through a mesh filter cloth, and the filtrate was aged for 7 days at 4-25 ° C., and then filtered using Whatman No. 2 filter paper. Add the extract to a 3 L separatory funnel, add 1 L of ethyl acetate, shake well, mix well, separate the mixture into two layers, take the upper layer (ethyl acetate layer), and lower the funnel (water layer) again. Extracted twice more. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 10.5 g.

Example  3: Sheep  Preparation of Stem Bark Extract

The stem bark of the yams (kenaf) was washed with purified water and dried, and then 100 g of the finely chopped sheep eggplant flour was put in 1 L of 50% ethanol aqueous solution, extracted by boiling in an extractor with a cooling condenser for 12 hours, and filtered through a 300 mesh filter cloth. The filtrate was left to mature at 4-25 ° C. for 7 days and then filtered through Whatman No. 2 filter paper. Put the extract in a 3 L separatory funnel, add 1 L of ethyl acetate, shake well, mix well, separate completely into two layers, take the upper layer (ethyl acetate layer), and place the lower layer (water layer) again with the separatory funnel. Extracted once more. The combined upper layers were combined, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 8.7 g.

Test Example  1: wrinkle improvement effect

As a wrinkle improvement effect of the extract of the sheep (Kenaf), in order to see the skin cell proliferation effect, human normal fibroblasts were added to each well of a 96-well microplate. Inoculated to 10 × ⁴ cells were incubated for 24 hours in DMEM (dulbecco's Modified Eagle's Medium) medium. After the cultivation, the amount of the yam (kenaf) leaf extract of Example 1, the yam (kenaf) eggplant extract of Example 2, and the yam (kenaf) stem bark extract of Example 3 were prepared so as to have a final concentration of 500 ㎍ / ml. Incubated for 24 hours more after replacement with adjusted serum-free DM medium. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium (MTT: (3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium: 5 mg / ml) was added 10 μl and left for 4 hours, and then the medium portion was discarded, and 100 μl of dimethyl sulfoxide solution was added to each well, and the absorbance at 570 nm was measured with a microplate reader. The cell proliferation effect was calculated from Equation 1 and the results are shown in Table 1.

&Quot; (1) "

% Cell proliferation effect = [(absorbance-control absorbance at extract treatment) / absorbance of control] × 100

Wrinkle improvement effect Sample (500 μg / ml) Absorbance (570 nm) Cell proliferation effect (%) Control group 0.310 - Example 1 0.450 45.1% Example 2 0.414 33.5% Example 3 0.494 59.3%

As shown in Table 1, it was found that all three extracts of horses (Kenaf) showed normal fibroblast cell proliferation effect. In particular, the effect of the stem bark extract was the best.

Test Example  2: collagen biosynthesis promoting effect

In order to investigate the collagen biosynthesis-promoting effect of the hemp (Kenaf) extract, human normal fibroblasts were inoculated to be 1 × 10 ⁴ cells in each well of a 96-well microplate. Incubated for 24 hours in DMEM (dulbecco's Modified Eagle's Medium) medium. After cultivation, the final extract of bark (kenaf) leaf extract of Example 1, the bark extract of the yeast (Kenaf) eggplant extract of Example 2 and the bark extract of the stem of the barn (Kenaf) stem of Example 3 were obtained at a final concentration of 500 ㎍ / ml. Incubate for 48 hours more after replacement with DM medium without serum. Collagen synthesis was promoted by adding 50 μg / ml of ascorbic acid before the last 24 hours of culture. After incubation, each well was washed, replaced with DM medium without serum, and further incubated for 24 hours. After incubation, the supernatant of each well was collected, and the amount of newly synthesized collagen was measured by the amount of PICP using a kit (Kit, Takara, Kyoto, Japan) using procollagen type I-peptide (PICP). PICP amount is shown in Table 2 below to synthesize the collagen in terms of ng / 2 × 10 ⁴ cells.

Collagen biosynthesis promoting effect Sample (500 μg / ml) Collagen Synthesis Amount (ng / 2 × 10 ⁴ ) Control group 140 Example 1 220 Example 2 189 Example 3 190

As shown in Table 2, all three sheep (Kenaf) extract was found to exhibit normal fibroblast cell proliferation effect. In particular, the yam extract (Kenaf) leaf extract was the most effective than the other extracts.

Hereinafter, on the basis of the results of the above test example, by containing at least one of the extract of the yam, the extract of the yam branch and the bark extract of the yam stem, various formulations having an anti-aging effect excellent in promoting the promotion of skin cells and collagen biosynthesis Present the composition of the cosmetics. However, the composition of the present invention is not limited only to the following examples.

Formulation Example  1: Softener (skin lotion)

Softening Cosmetic Ingredient Compounding ingredient weight% Purified water To 100 Hemp leaf extract, hemp eggplant extract and / or bark stem extract 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount

Formulation Example  2: nutrition cosmetics Milk lotion )

Nutritional Cosmetic Ingredients Compounding ingredient weight% Purified water To 100 Hemp leaf extract, hemp eggplant extract and / or bark stem extract 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

Formulation Example  3: Nourishing cream

Nutrition Cream Ingredients Compounding ingredient weight% Purified water To 100 Hemp leaf extract, hemp eggplant extract and / or bark stem extract 0.1 Wax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

Formulation Example  4: massage cream

Massage Cream Ingredients Compounding ingredient weight% Purified water To 100 Hemp leaf extract, hemp eggplant extract and / or bark stem extract 0.1 Wax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

Formulation Example  5: Pack

Pack compounding ingredient Compounding ingredient weight% Purified water To 100 Hemp leaf extract, hemp eggplant extract and / or bark stem extract 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingzi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount

Claims (13)

Ethanol was added to the ground bark stalks, extracted for 12 hours, and the filtered filtrate was left to mature at 4-25 ° C. for 7 days, aged, filtered and mixed with ethyl acetate. After taking the upper layer, the separated upper layer was concentrated under reduced pressure at 50 ℃ cosmetic composition having a wrinkle-improving effect containing the sheep extract as an active ingredient. delete The cosmetic composition according to claim 1, wherein the wrinkle improvement effect is a skin cell proliferation effect. delete delete delete delete delete The cosmetic composition according to claim 1, wherein the sheep's horse extract is contained in an amount of 0.0001 to 10% by weight based on the total weight of the cosmetic composition. delete According to claim 1, wherein the cosmetic is a softening cream, astringent makeup, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream Cosmetic body, characterized in that it has a formulation selected from the group consisting of body oil and body essence. delete delete