KR20110091137A - Cosmetic composition comprising the extract of liriodendron tulipifera as active ingredient - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Liriodendron tulipifera extract is provided to ensure anti-aging, antioxidation, skin regeneration, anti-inflammation, and moisturizing. CONSTITUTION: A cosmetic composition for suppressing sebum secretion or enhancing skin moisturizing, skin regeneration, anti-wrinkling, and antioxidation contains 0.00001-30.0%(w/w) of Liriodendron tulipifera extract as an active ingredient. The extract is isolated by sonication, supercritical extraction, and fermentation using glycerine, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, dichloromethane, hexane, or mixture thereof. The cosmetic composition is manufactured in the form of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, or spray.

Description

Cosmetic composition containing lily extract as an active ingredient {Cosmetic Composition Comprising the Extract of Liriodendron tulipifera as Active Ingredient}

The present invention relates to a cosmetic composition containing the lily extract as an active ingredient.

Skin is composed of epidermal layer, dermis layer and subcutaneous fat layer. The most basic role is to prevent evaporation of moisture from our body and to prevent harmful components from invading from the outside. Since these skins are always exposed to external stimuli, they have a variety of defenses. In addition, skin is a sign of beauty, and it can be said that the health and cleanness of skin is related to human beauty.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into internal aging and photo-aging, and research on this has been actively conducted. Free radicals may be activated by UV rays, stress, disease conditions, environmental factors, wounds, and age. If this condition is exacerbated, it destroys antioxidant defenses in vivo and damages cells and tissues. And aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins is caused by severe cuts in the connective tissues of collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, and so on, resulting in severe over-inflammatory reaction and skin elasticity. If this worsens, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

Therefore, it protects the cell membrane by eliminating the free radicals caused by metabolic processes of the body, irradiation by UV irradiation and inflammatory reactions, and the already damaged cells should be regenerated by active metabolism to proliferate the cells. You can recover quickly and maintain healthy skin.

In recent years, as well as women, men have been interested in various skin problems such as skin aging has been developed a lot of related products. As a person ages, skin aging occurs. The main symptom is wrinkles. Wrinkles are a phenomenon of age, and they appear on our faces. A representative cause of wrinkles is when collagen, which forms a matrix in the dermis of the skin, breaks down and fails to function. Therefore, a representative method for improving wrinkles is to promote the production of collagen constituting the dermal matrix or to inhibit the production or activity of collagen degrading enzyme MMPs (MatrixMetalloProteinase). In the cosmetics industry, a lot of research has been conducted on these contents, and most wrinkle improving functional cosmetics have been applied to products with these effects. In general, conventional substances that promote collagen synthesis include retinoids (RE36068), TGF-β (Transforming growth factor), betulinic acid (JP8-208424), and the like. TGF- inhibits the production of MMP-1. β (Transforming growth factor) is known. In addition, various natural products are known to promote collagen synthesis or inhibit MMP-1 biosynthesis. Therefore, in order to suppress wrinkle formation, a method of promoting collagen synthesis or inhibiting the production or activity of collagenase may be used.

As human skin ages, wrinkles naturally occur on the skin. However, these wrinkles are also exposed to ultraviolet rays or exposed to external pollutants for a long time, the formation rate is accelerated to deepen and increase the wrinkles. The main causes of wrinkles include decreased collagen synthesis and degradation. As a method for suppressing wrinkle formation, there is generally a method of inhibiting wrinkle formation by promoting collagen synthesis, or inhibiting wrinkle formation by inhibiting generation or activity of collagenase.

In addition, the inflammation of the skin is caused by misuse, abused drugs or ultraviolet rays from the sun is known to cause inflammation by the signaling system of the immune response system. In addition, ingredients used in cosmetics may cause an allergic reaction or skin skin irritation in some cases.

Cosmetics are defined as a slight effect of applying to the skin for the protection, beautification and cleansing of the skin. Among the ingredients used in these cosmetics, preservatives, surfactants, pigments, UV absorbers and other ingredients are rather It can cause skin irritation by irritating skin cells or causing allergic reactions.

This stimulus leads consumers to disbelief in cosmetics and have a sense of rejection. Therefore, cosmetics researchers are conducting a lot of research to eliminate such inflammation and skin irritation, and are using pure substances derived from nature.

In order to prevent skin inflammation, allantoin, bisabolol, etc. were used in cosmetics. However, allantoin has a poor solubility in water and oils, and thus it is limited to use in the product. In the case of bisabolol, allantoin is known to have an excellent anti-inflammatory effect but cause side effects.

In order to solve the problem of the skin, a lot of cosmetics using natural products have recently been developed to reduce skin irritation caused by various chemicals. Natural materials have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing.

We tried to find a substance that improves wrinkles caused by aging by using an active ingredient separated from natural products for the purpose of improving skin wrinkles. Lily extract extracted from Magnolia and Liriodendron tulipifera In addition to the anti-wrinkle effect, it has antioxidant, anti-aging, skin cell regeneration, skin irritation, skin moisturizing, anti-inflammatory, anti-ultraviolet damage, sebum secretion, collagen synthesis and collagen production. The present invention has been found to effectively improve and prevent skin conditions.

As described above, the present invention is very effective in improving skin wrinkles, anti-oxidation, anti-aging skin, regenerating skin cells, relieving skin irritation, skin moisturizing, anti-inflammatory, UV damage, and sebum secretion by adding lily extract to cosmetics. Relates to a good cosmetic composition.

Accordingly, it is an object of the present invention to provide a cosmetic composition for improving skin conditions containing a lily extract as an active ingredient.

In addition, the present invention is applied to the skin of the cosmetic composition to improve the skin wrinkles, as well as anti-oxidation, skin aging, skin cell regeneration, skin irritation relief, skin moisturizing, anti-inflammatory, inhibiting damage by UV rays and sebum secretion effect To provide a make-up method to achieve.

Other objects and advantages of the present invention will become apparent from the following solutions, examples and claims.

The present invention relates to a cosmetic composition for improving skin conditions containing a lily extract as an active ingredient. More specifically, the present invention contains a lily extract as an active ingredient, such as skin wrinkle improvement, antioxidant, skin aging prevention, skin cell regeneration, skin irritation relief, moisturizing, anti-inflammatory, inhibiting damage caused by ultraviolet rays, sebum secretion, etc. It is effective, promotes the synthesis of skin cell proteins such as collagen, and effectively inhibits the biosynthesis of collagen degrading enzymes that are increased by ultraviolet rays, thereby improving skin troubles caused by aging and preventing skin aging. It relates to a composition.

The present invention relates to a cosmetic composition containing a lily extract. More specifically, the present invention is a magnolia and a lily tree Liriodendron It relates to a cosmetic composition for improving skin condition containing a lily extract made using an active ingredient extracted from tulipifera ).

Liriodendron tulipifera is a magnolia and plant native to North America. It is about 13m high and its bark is brown with ash and black color. Leaves are alternate, wide, round egg-shaped, about 6-18cm in length and butterfly. Flowers bloom greenish yellow in May-June, with one tulip-like flower about 6cm in diameter. 3 sepals and 6 petals. The base of the petal has an orange pattern. There are many pistils and stamens, and after flowering, the jaws grow about 7cm long. Fruits are lungs, ripen in October-November, with wings and 1-2 seeds. In the United States, because of its rapid growth, it is used as an important spring water (用 材 樹), but in sub-central Korea, it is planted for ornamental purposes.

  In the present specification, the lily tree extract used as an active ingredient of the cosmetic composition means an extract obtained from various parts of the lily tree (eg, stems, branches, roots, leaves, flowers, seeds, etc. of the lily tree). Means an extract extracted from the branch of a lily tree.

The lily extract of the present invention can be prepared using conventional solvents (e.g., water, anhydrous or hydrous lower alcohols) according to conventional methods known in the art, i.e., under conditions of conventional temperature and pressure. , Acetone, ethyl acetate, butyl acetate or 1,3 butylene glycol).

According to a preferred embodiment, the method of producing a lily tree extract of the present invention is as follows. First, the lily branches are washed with purified water, dried and crushed into small pieces, followed by 1 to 10 times the dry weight of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate or 1,3-butylene glycol, etc. are added. After the cooling condenser is installed to prevent the active ingredient is evaporated in the state of extraction for 3 to 20 hours by heating at 40 to 100 ℃, or extracted for 1 to 15 days at 4 to 40 ℃ and completely dried by a rotary vacuum evaporator to prepare . In this case, since 1,3-butylene glycol is difficult to dry using a rotary vacuum evaporator, the extraction loss is directly adjusted to 1% (w / v) and then used in the cosmetic of the present invention.

Meanwhile, the lily tree extract of the present invention includes not only the extract by the above-described extraction method, but also an extract by supercritical extraction or ultrasonic extraction by reduced pressure and high temperature with carbon dioxide.

The decompression by carbon dioxide and the supercritical extraction by high temperature mean supercritical fluid extraction. Generally, the supercritical fluid is a liquid having a gas when the gas reaches a critical point under high temperature and high pressure. It is gaseous, chemically similar in polarity to nonpolar solvents, and because of its properties, supercritical fluids are used for extraction of fat-soluble substances (J. Chromatogr. A. 1998; 479: 200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gaseous properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

After extracting the fat-soluble substance, the supercritical carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction container to extract components that were not extracted with pure supercritical carbon dioxide alone.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using a supercritical carbon dioxide or a mixed fluid in which a co-solvent is added to carbon dioxide. The cosolvent may be used one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

As the extraction solvent that can be used in the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used.

The extracted sample is vacuum filtered to recover the filtrate, and then removed by a vacuum evaporator, the extract can be obtained through a conventional extract preparation method of freeze drying.

In addition, the lily extract of the present invention also includes extracts that have undergone conventional purification and / or fermentation. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also construed to be included in the lily extract of the present invention. In addition, the yeast, lactic acid bacteria, Bacillus genus and a mixture selected from the group of fermented products obtained by fermentation for 1 to 7 days while maintaining a pH of 5 to 7 under a temperature condition of 20 to 40 ℃ also the lily tree of the present invention Interpreted in the extract.

In addition, the lily tree extract of the present invention also includes an extract obtained by using the foaming method. Foaming agent is a pharmaceutical technology that changes the original properties of the medicine by processing the medicine based on the herbal theory. Mainly refers to the process of steaming, boiling, roasting, roasting or roasting or a process in which these are mixed. The treatment conditions are 60-100 ° C. for 30 minutes to 6 hours when boiled, 120 to 180 ° C. for 10 minutes to 2 hours for steaming, and 80-100 ° C. for 10 minutes to 2 hours for baking. In the present invention, the lily tree was steamed and then used to prepare an extract using the same. After extracting by using the foaming method to steam and dry the lily tree can be extracted not only by solvent extraction method, but also by extraction method by supercritical extraction or ultrasonic extraction by reduced pressure with carbon dioxide and high temperature, 70% in the embodiment of the present invention Extraction using ethanol was used for the experiment.

Lily tree extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to a more preferred embodiment of the present invention, the lily extract of the present invention has an antioxidant effect (Experimental Example 1), cell regeneration effect (Experimental Example 2), cell stimulating effect (Experimental Example 3), cytotoxicity by ultraviolet irradiation The mitigating effect (Experimental Example 4), anti-inflammatory effect (Experimental Example 5), MMP-1 production inhibitory effect (Experimental Example 6), and collagen synthesis enhancing effect (Experimental Example 7) are excellent.

As used herein, the term "improvement of skin condition" refers to the improvement of various skin conditions, which improve skin conditions, for example, anti-aging, skin wrinkle improvement, skin moisturization, etc. Secondary skin conditions to prevent skin aging, such as suppressing or improving skin allergies and skin inflammatory responses, such as anti-inflammatory, UV-inhibiting skin damage, cell regeneration and suppressing sebum secretion, or inhibiting collagen degrading enzymes and enhancing collagen synthesis Includes all improvements. In addition, as the scalp also corresponds to the skin, the scalp moisturizing, antioxidant, anti-inflammatory, cell renewal, sebum secretion inhibitory effect is also included in improving the skin condition. Therefore, the cosmetic for improving the skin condition of the present invention is for skin wrinkle improvement, anti-oxidation, skin cell regeneration, anti-inflammatory, anti-skin damage, anti-aging, sebum secretion and skin moisturizing. , Cosmetics.

Such a lily extract of the present invention can obtain the effect of improving the skin condition as described above even when manufactured in a cosmetic composition.

According to a preferred embodiment of the present invention, the content of the lily tree extract of the present invention is 0.00001 to 30.0% by weight, preferably 0.0001 to 20.0% by weight relative to the total weight of the cosmetic composition. At this time, when the total weight of the lily extract is less than 0.00001% by weight, the effect is less likely to appear, when it exceeds 30.0% by weight, it is highly likely to cause irritation to the skin and may have a great influence on the stabilization of the formulation.

The components included in the cosmetic composition of the present invention is an active ingredient, in addition to the lily extract, various components that maintain the activity of biological components, such as preservatives, osmotic agents, pH adjusting agents, buffers, stabilizers, aluminum hydroxide, aluminum phosphate, interfacial Active agents, liposomes, thickeners, and the like, and ingredients commonly used in cosmetic compositions such as antioxidants, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments, and flavors, and carriers, and the like. Do not.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing It may be formulated as, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More preferably, it may be prepared in the formulation of basic cosmetics such as flexible lotion (skin), astringent lotion, nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, pack.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. Fatty acid esters of glycol, 1,3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester as carrier components , Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In case the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. This can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro Propellant such as carbon, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, or a sarcosinate. Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters, and the like.

According to a more preferred embodiment of the present invention, a cosmetic containing the lily extract of the present invention was prepared as an active ingredient, the cosmetic of the present invention has a skin wrinkle improvement effect (Experimental Example 8), skin elasticity improvement effect (Experimental Example) 9), sebum secretion inhibitory effect (Experimental Example 10) and skin moisturizing effect improvement effect (Experimental Example 11), as well as antioxidant effect (Experimental Example 1), cell regeneration effect ( Experimental Example 2), cell stimulation alleviation effect (Experimental Example 3), photoaging prevention effect (Experimental Example 4), anti-inflammatory effect (Experimental Example 5), and inhibition of MMP-1 production (Experimental Example 6) and enhancement of collagen synthesis (Experimental Example) 7) anti-aging effect can be expected.

The present invention also provides a cosmetic method comprising applying a cosmetic composition containing the active ingredient of the present invention to human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap or oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel or cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water or cleansing gel, but is not limited thereto.

According to a preferred embodiment of the present invention, when the cosmetic method of the present invention is applied directly to the skin of a person, an excellent skin wrinkle improvement effect; Antioxidative effect; Cell regeneration effect; Skin cell stimulating alleviation effect; Cytotoxic alleviation effect by ultraviolet irradiation; Anti-inflammatory effect; Improving skin elasticity; Inhibiting collagenase, enhancing collagen synthesis and preventing skin aging; Sebum skin moisturizing effect; And skin moisturizing effects.

< Example >

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Lily Tree Extract Preparation I

To 200 g of dried lily-tree branches, 1000 ml of 70% ethanol with the ratio of water and ethanol adjusted to 7: 3 was added and leached at room temperature for 3 days. It was filtered and the filtrate was concentrated in vacuo to give a dry extract, the extraction yield was 44.8g / 200g. The dry extract was dissolved in 50% 1,3-butylene glycol solution to 1% (w / v) concentration and filtered and then subjected to the experiment of the present invention.

Example 2-16. Preparation of Lily Tree Extract II

Examples 2 to 16 were extracted in the same manner as in Example 1 except that the extraction solvent of Table 1 was used to obtain a lily tree extract. In addition, Example 15, Example 16 obtained the lily extract through the extraction using conventional supercritical extraction, ultrasonic and conventional methods. However, in the case of Example 15, olive oil was used as the cosolvent and is a liquid.

Extraction Yield of Lily Tree Extract Extraction solvent From 1 kg of lily
Weight of extract obtained in g Example 2 Purified water 40.5 Example 3 25% ethanol 42.6 Example 4 50% ethanol 43.8 Example 5 100% ethanol 45.8 Example 6 80% methanol 46.7 Example 7 100% methanol 43.3 Example 8 Acetone 44.1 Example 9 Diethyl ether 43.3 Example 10 Hexane 26.3 Example 11 Normal-propanol 23.5 Example 12 Isopropanol 26.5 Example 13 Normal-butanol 25.7 Example 14 Ethyl acetate 23.5 Example 15 Supercritical Extraction 146.8 Example 16 Ultrasonic extraction 36.3

Example 17. Preparation of Lily Tree Ferment Extract

After washing with purified water and drying the dried branch of a lily tree was made fine by using 300 mesh. This was added to the yeast culture (Potato Dextrose Broth, Accumedia, United States) to 10 g / L. Saccharomyces cerevisiae was used as a yeast strain for fermentation, and was added at 50,000 cfu / L per culture. Cultivation was carried out using 5L fermenter for 7 days, maintained at 30 ℃, pH 5-7. The cultured lily fermentation broth was centrifuged to remove cultures first, and then filtered through 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days in a low temperature warehouse at 4 ° C., and then the filtrate was filtered again using a filter of 0.25 μM and used in the present invention. Called. In addition, the extraction yield by this method was 402.8 g / 1 kg. The lily fermentation extract used in the experiment below was used to maintain a final concentration of 1g / 100mL.

Example 18. Preparation of the extract of the lily tree by the preparation method

In the embodiment of the present invention to obtain a sire extract of the lily tree was processed by steaming and drying. In other words, after washing the dried lily tree powder with purified water and finely made by using a 300 mesh, steaming was carried out under a condition of 60 ℃ ~ 150 ℃, 10 minutes ~ 2 hours and then dried. Thus prepared extract of the lily tree was extracted using additional 70% ethanol, the result was obtained 46.8g / 1kg extract.

Experimental Example 1. Antioxidative Effect of Lily Tree Extract

In this experiment, the positive effect of the vitamin C, a substance known to have an antioxidant effect, in order to confirm the antioxidant effect of the lily extract obtained in Examples 1, 15, 16, 17, and 18, Active oxygen scavenging rate).

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by NBT method, and the test substance (lily extract, vitamin C) removes free radicals, that is, the free radical scavenging rate ( %) Was evaluated. do. Free radicals are reacted with Nitro Blue Tetrazolium (NBT) to determine the percentage of active oxygen scavenging (%) by measuring the resulting blue at wavelength 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to the vial, and add 0.1ml of sample solution to it and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

(4) A blank of the sample solution is measured in the same manner as in the case of measuring the absorbance St, except that purified water is used instead of the above 6, and the absorbance So is measured.

⑤ The absorbance test is performed in the same manner as in the case of measuring the absorbance St, except that the purified water is used instead of the sample solution.

⑥ The blank of the blank test was measured in the same manner as when the absorbance St was measured except for using the sample solution and purified water instead of 6, and the absorbance Bo was measured.

Active oxygen scavenging rate (%) was calculated by Equation 1, the results are shown in Table 2.

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme in sample solution

Bo: absorbance at 560 nm before reaction in the absence of enzyme in blank test solution

Antioxidant Effect of Lily Extract division Concentration (%, w / v) Antioxidative effect(%) Example 1 (70% Ethanol Extract of Lilium) 1.0 73.8 Example 15 (Supercritical Extract of Lilium) 1.0 60.8 Example 16 (Ultrasonic Extract of Lily) 1.0 70.2 Example 17 (Iliax Ferment Extract) 1.0 75.6 Example 18 (extract of the lily tree by the foaming method) 1.0 77.8 Vitamin c 1.0 66.8

As can be seen from the results of the active oxygen scavenging rate of Table 2, the lily extract of the present invention obtained through various extraction methods is similar to vitamin C (1% by weight) known to have an antioxidant effect by any method or It was found to have a much better antioxidant effect.

Experimental Example  2. Lily tree  Cell Regeneration Effect of Extracts

37 ° C humidified by inoculating HaCaT keratinocytes (German Cancer Research Institute, Germany) with DMEM (Dulbeco's Modified Eagle's Medium, Gibco) with 10% FBS at a density of 2 × 10 ⁵ cells / well , 1 day incubation in a 5% CO ₂ incubator. After exchanging with serum-free DMEM, samples of the lily extracts were treated with 1.0% (v / v) and incubated for 3 days. At this time, the control (negative) was carried out using saline instead of lily extract. After culturing, the production of cells was compared and evaluated using MTT assay, and TGF-β 10ng / mL was used as a positive control, and the results are shown in Table 3 below.

Cell Proliferation Effect of Lily Extract division  Abs. at 570nm Control group 0.954 Example 1 (70% Ethanol Extract of Lilium) 1.268 Example 15 (Supercritical Extract of Lilium)  1.254 Example 16 (Ultrasonic Extract of Lily)  1.084 Example 17 (Iliax Ferment Extract)  1.055 Example 18 (extract method extract of lily of the valley)  1.268 Positive control substance (TGF-β (10ng / ml))  1.468

As can be seen from the results of the cell activity test of Table 2, it was confirmed that the lily extract of the present invention obtained through various extraction methods has an excellent cell regeneration effect by any method.

Experimental Example 3. Relaxation effect of cellulite extract of lily tree extract

Fibroblasts (Korea Cell Line Bank, South Korea), which are human skin cells, were cultured in T-75 flasks (Falcon, United States) until they grew about 80%. This was again transferred to 3 × 10 ⁴ cells / well in a 96-well plate (Falcon, USA) and incubated for 24 hours. After culturing, the microscope confirmed whether the cells were completely attached and grew well. The experiment was performed using lactic acid as a source of skin cell stimulation. At this time, lactic acid was used 2.0%. That is, 200 μl of DMEM (Sigma, United States) medium containing 2.0% lactic acid was added to each of 96 wells, and 1.0% (v / v) of the lily extract or fermentation broth of Examples 1, 15, 16, 17, and 18 was added. Adjusted to). At this time, all of the lactic acid and lily extract were not added as a control (negative), and only the lactic acid was added as a comparison group. In order to compare the viability of the cells 12 hours after the addition of the test substance, the cell viability was measured by adding MTT (Sigma, US) solution (3 mg / ml) at 570 nm using ELISA READER (Molecular Devices, USA). And cell viability (%) was calculated according to the following equation 2 and the experimental results are shown in Table 4 below.

Cell Stimulation Relaxation Effects of Lily Tree Extracts division Cell survival rate (%) Control group 100.0 Lactic acid 2.0% 12.6 Lactic Acid 2.0% + Example 1 (70% Ethanol Extract of Lily of the Valley) 56.8 Lactic Acid 2.0% + Example 15 (Supercritical Extract of Lilium) 62.5 Lactic Acid 2.0% + Example 16 (Ultrasonic Extract of Lily) 66.4 Lactic acid 2.0% + Example 17 67.5 Lactic acid 2.0% + Example 18 (extract of the lily tree by the formulation method) 60.2

As can be seen from the results of Table 4, the lily tree extract of the present invention obtained through a variety of extraction methods by any extraction method to alleviate the cytotoxicity caused by lactic acid treatment significantly increased the cell survival rate, the difference between the extraction method The effect of the extract on the cell stimulation was found to be excellent without significant difference.

Experimental Example 4. Cytotoxicity-Reducing Effect of Lily Extracts by UV Irradiation

This experiment was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the lily tree extract obtained in Examples 1, 15, 16, 17 and 18. In brief, fibroblasts were placed in a 24-well test plate 5 × 10 ⁴ and attached for 24 hours. Each well was washed once with PBS and 1000 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B15W, Sankyo Dennki, Japan), after which PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. It was incubated for 24 hours after the lily tree extract treatment. At this time, the lily extract used in the experiment was used in each concentration of 1.0%. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed and 500 μl of isopropanol-HCl (0.04N) was added thereto. The cells were lysed by shaking for 5 minutes and 100 μl of supernatant was transferred to a 96-well test plate, followed by measurement of 570 nm absorbance in a microplate reader. At this time, TGF-β (10ng / ml) was used as a positive control, and saline was used as a negative control instead of a lily tree extract. Cytotoxicity relaxation rate (%) by ultraviolet irradiation was calculated by the following equation (3), the results are shown in Table 5.

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: cell viability of wells that were not irradiated with UV light

St: Cell survival rate of wells treated with UV

Cell Protection Effect of Ultraviolet Radiation from Lily Extract division Cell survival rate (%) Control (negative) 12.8 Example 1 (70% Ethanol Extract of Lilium) 62.8 Example 15 (Supercritical Extract of Lilium) 66.4 Example 16 (Ultrasonic Extract of Lilium) 60.7 Example 17 (Iliax Ferment Extract) 62.6 Example 18 (Yellow extract by the formulation) 60.4 Positive control (TGF-β (10ng / ml)) 80.6

According to the cytotoxicity relaxation results of Table 5, the lily extract of the present invention obtained through various extraction methods was found to have an excellent cytotoxicity relaxation effect by any method. In addition, each cell viability has a cell survival rate close to the value of TGF-β which is known to have a good cytotoxic mitigating effect, it can be seen that the lily extract of the present invention effectively protects against cell damage by UV irradiation there was.

Experimental Example 5. Inhibitory Effect of Lily Tree Extract on Inflammatory Cytokine Expression

This experiment is to evaluate the inhibitory effect of inflammatory cytokine expression expressed by ultraviolet irradiation to the lily tree extract obtained in Examples 1, 15, 16, 17, and 18, and fibers isolated from human epidermal tissue. Fibroblasts were placed in 5 × 10 ⁴ cells in 24-well test plates and then attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with 10 mJ / cm 2 of UV light using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), after which PBS was removed and FBS was not added to the cell culture medium (DMEM). Medium) 350 μl was added. Here, the lily extract to be evaluated was treated with 1.0% (w / v) and incubated for 5 hours. 150 µl of the culture supernatant was taken to quantify IL-1α to determine the effect of inhibiting the inflammatory cytokine expression of the lily extract. The amount of IL-1α was quantified using an enzyme-linked immunosorbent assay, and ketoprofen, known as an IL-1α inhibitor, was used as a positive control. Inhibition rate (%) of inflammatory cytokine (IL-1α) was calculated by Equation 4 below, and the results are shown in Table 6 below.

Bo: IL-1α production in wells without UV irradiation

Bt: IL-1α production in wells that were not irradiated with UV light

St: IL-1α production amount of wells that were irradiated with UV light

Anti-inflammatory Effect of Lily Extract division Inflammatory cytokines
% Inhibition of expression Example 1 (70% Ethanol Extract of Lilium) 56.3 Example 15 (Supercritical Extract of Lilium) 55.2 Example 16 (Ultrasonic Extract of Lily) 50.8 Example 17 (Iliax Ferment Extract) 48.6 Example 18 (extract of the lily tree by the foaming method) 45.4 Positive control (ketoprofen (100 μg / ml)) 66.8

According to the results of Table 6, the lily extract of the present invention obtained through various extraction methods, it can be seen that by any extraction method, inhibits the production of inflammatory cytokines IL-1α by ultraviolet light. In particular, compared with ketoprofen, a positive control known to have an inhibitory effect on the production of the inflammatory cytokine IL-1α, the above-described excellent inhibition rate was observed even when the ketoprofen concentration (100 µg / ml) was different. It can be seen that the lily extract of the present invention effectively protects the occurrence of inflammation caused by ultraviolet rays even at low concentrations.

Experimental Example 6. Inhibitory Effect of Lily Extract on MMP-1 Production

This experiment was conducted using the sample obtained in the above example. Each sample was dissolved in 1% (w / v) dry weight in 50% 1,3-butylene glycol solution and used in this experiment. The experiment proceeded as follows. First, human normal fibroblasts were seeded to 1 × 10 ⁵ cells in each well of a 48-well microplate (Nunc, Denmark) and incubated for 24 hours at 37 ° C. in DMEM medium (Sigma, USA). Subsequently, the experiment of replacing the serum-free DMEM medium with the final concentration of the lily-tree extract at 1.0% and the control group replaced with the serum-free DMEM medium not containing the lily extract was further incubated for 48 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, US), and then the amount of MMP-1 was converted to ng / ml. TGF-β (10 ng / ml, Roche, United States) was used as a positive control, MMP-1 production inhibition was calculated according to the following equation (5). The results are shown in Table 7.

Inhibitory Effect of Lily Extract on MMP-1 Production division MMP-1 production inhibitory effect (%) Example 1 (70% Ethanol Extract of Lilium) 75.8 Example 15 (Supercritical Extract of Lilium) 74.5 Example 16 (Ultrasonic Extract of Lily) 77.2 Example 17 (Iliax Ferment Extract) 74.6 Example 18 (extract of the lily tree by the foaming method) 70.6 Positive Control TGF-β (10 ng / ml)  76.8

As can be seen from the results of Table 7, the lily extract of the present invention obtained through various extraction methods, by any extraction method, excellent activity similar to TGF-β, a positive control in MMP-1 production inhibition rate I could see that.

Experimental Example 7 Collagen Synthesis Enhancement Effect of Lily Tree Extract

Human normal fibroblasts were seeded to 1 × 10 ⁵ cells in each well of a 48-well microplate and incubated for 24 hours at 37 ° C. in DMEM medium. Subsequently, the experimental group replaced with serum-free DMEM medium at 50 μg / ml and the control group replaced with serum-free DMEM medium containing no lily extract were further incubated for 24 hours. After incubation, the supernatant of each well was collected and the amount of newly synthesized collagen was measured using a procollagen type I C-peptide (PICP) kit (Takara, Japan). PICP amount was converted to ng / ml, and TGF-β (10ng / ml) was used as a positive control. Collagen biosynthesis increase rate was calculated by the following equation 6, the results are shown in Table 8.

Enhancement of Collagen Synthesis by Lily Extract division Collagen Synthesis Effect (%) Example 1 (70% Ethanol Extract of Lilium) 160.8 Example 15 (Supercritical Extract of Lilium) 158.6 Example 16 (Ultrasonic Extract of Lily) 156.7 Example 17 (Iliax Ferment Extract) 160.8 Example 18 (extract of the lily tree by the foaming method) 158.4 Positive Control TGF-β (10 ng / ml) 166.8

As can be seen from the results of Table 8, the collagen synthesis enhancing effect of the lily tree extract of the present invention has a similar effect to the positive control TGF-β, and, in addition to the conventional extraction method using various extraction solvents, supercritical It was found that any extract extracted by the extraction method, the ultrasonic extraction method, the fermentation extraction method or the foaming method has a collagen synthesis enhancing effect of similar activities.

<Prescription>

The following prescription examples are given, but the present patent is not limited to these prescription examples. In addition, the lily extract was used to dissolve the 1% (w / v) of the lily extract prepared in Example 1 in 50% 1,3-butylene glycol.

Formulation Example 1. Soft Cosmetics

Prescription examples of flexible cosmetics in cosmetics containing a lily extract are as follows.

ingredient Content (unit: weight%) Prescription Example 1 Comparative Prescription 1 Comparative Prescription 2 Lily Tree Extract 2.0 - - White bran extract - Purified water 2.0 Purified water - - 2.0 glycerin 5.0 5.0 5.0 1.3-butylene glycol 3.0 3.0 3.0 Fiji 1500 1.0 1.0 1.0 Allantoin 0.1 0.1 0.1 DL-Panthenol 0.3 0.3 0.3 E.T.A.-2NA 0.02 0.02 0.02 Benzophenone-9 0.04 0.04 0.04 Sodium hyaruronate 5.0 5.0 5.0 ethanol 10.0 10.0 10.0 Octicdodeceth-16 0.2 0.2 0.2 Polysorbate 20 0.2 0.2 0.2 Preservative, fragrance, coloring a very small amount a very small amount a very small amount Purified water Balance Balance Balance Sum 100 100 100

Prescription Example 2. Converging Cosmetics

Prescription examples of astringent cosmetics in cosmetics containing a lily extract are as follows.

ingredient Content (unit: weight%) Lily Tree Extract 1.0 glycerin 2.0 1.3-butylene glycol 2.0 Allantoin 0.2 DL-Panthenol 0.2 E.T.A.-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch hazel extract 2.0 Citric acid a very small amount Preservative, fragrance, coloring a very small amount Purified water Balance Sum 100

Prescription Example 3. Nutritional Cosmetics

Prescription examples of nutrient cosmetics in cosmetics containing a lily extract are as follows.

ingredient Content (unit: weight%) Lily Tree Extract 1.5      Glyceryl Stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxy Vinyl Polymer 0.12 glycerin 5.0 1.3-butylene glycol 3.0 Sodium hyaluronate 5.0 Tri ethanolamine 0.12 Preservative, fragrance, coloring a very small amount Purified water Balance Sum 100

Prescription Example  4. Nutrition Cream

Prescription example of nutrition cream among cosmetics containing lily extract is as follows.

ingredient Content (unit: weight%) Prescription Example 4 Comparative Prescription 3 Comparative Prescription 4 Lily Tree Extract 3.0 - - Purified water - 3.0 - Adenosine - - 3.0 Lipophilic monostearate 2.0 2.0 2.0 Cetearyl alcohol 2.2 2.2 2.2 Stearic acid 1.5 1.5 1.5 Beeswax 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 Sorbitan stearate 0.6 0.6 0.6 Hardened vegetable oil 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 Mineral oil 5.0 5.0 5.0 Trioctanoine 5.0 5.0 5.0 Dimethicone 1.0 1.0 1.0 Sodium magnesium silicate 0.1 0.1 0.1 glycerin 5.0 5.0 5.0 Betaine 3.0 3.0 3.0 Triethanolamine 1.0 1.0 1.0 Sodium hyaluronate 4.0 4.0 4.0 Preservative, fragrance, coloring a very small amount a very small amount a very small amount Purified water Balance Balance Balance Sum 100 100 100

Prescription 5. Massage Cream

A prescription example of a massage cream in cosmetics containing a lily extract is as follows.

ingredient Content (unit: weight%) Lily Tree Extract 2.0     Lipophilic monostearic acid glycerin 1.5     Stearyl alcohol 1.5     Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservative, fragrance, coloring a very small amount Purified water Balance Sum 100

Prescription Example 6. Essence

Prescription example of the essence of the cosmetic containing the lily extract is as follows.

ingredient Content (unit: weight%) Lily Tree Extract 2.5 glycerin 10.0 Betaine 5.0 Fiji 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 E.T.A.-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth -16 0.4 ethanol 6.0 Preservative, fragrance, coloring a very small amount Purified water Balance Sum 100

Prescription 7.Pack Formulation

A prescription example of a pack of cosmetics containing a lily extract is as follows.

ingredient Content (unit: weight%) Lily Tree Extract 3.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 Fiji 1500 2.0 Cyclodextrin 0.15 DL-Panthenol 0.4 Allantoin 0.1 Monomethylammonium Glycyric Acid 0.3 Nicotinamide 0.5 ethanol 6.0 Fiji 40 Cured Castor Oil 0.3 Incense, pigment, preservative a very small amount Purified water  Balance Sum 100

Experimental Example 8. Evaluation of the wrinkle improvement effect of the cosmetic containing the lily extract

The skin wrinkle improvement effect of the cosmetic of this invention was evaluated through the actual use test. The nutrition cream containing 3% (v / v) of the lily tree extract of Formulation Example 4 and the cream obtained by replacing the lily tree extract with purified water in Formulation Example 4 were compared to Formulation Example 3, and the lily tree extract of Formulation Example 4 Was replaced with adenosine, a substance known to have an anti-wrinkle effect, was prepared in Comparative Preparation Example 4. Among 30 30-40 year old females, women who had a lot of external activities were randomly divided into three groups. It was corrected continuously for 2 months. Wrinkle improvement effect of each subject was evaluated through visual observation, and the effective rate (%) was obtained by dividing the number having excellent or slightly skin wrinkle improvement effect by the total number of subjects as a percentage, and the results are shown in Table 16 below. It was.

Skin Wrinkle Improvement Effect of Cosmetics Containing Lily Extract (Visual Evaluation) Skin wrinkle improvement Effective rate (%) Great slightly none Cream of the prescription example 4 (lily tree extract) 24 One 5 83.3 Cream (purified water) of the comparative example 3 4 3 23 23.3 Cream (adenosine) of the comparative example 4 25 2 3 85.3

According to the results of Table 16, the cosmetics containing the lily extract of the present invention not only showed an excellent skin wrinkle improvement effect compared to the cream of Comparative Formula 3, but also contains adenosine, a component known to have an anti wrinkle effect. It showed a similar effect to the cream of Comparative Example 4, and skin irritation could not be observed in the subjects to which the cosmetic of the present invention was applied to the skin.

Experimental Example 9. Improvement of Skin Elasticity of Cosmetics Containing Lily Extract

A clinical experiment was conducted on the skin elasticity improving effect of the cosmetics containing the lily of the valley extract of the present invention. Clinical trials were evaluated through the actual use test of each cosmetic.

In other words, Table 12, Comparative Example 3, Lily tree extract that replaced the lily extract with purified water in the nutritional cream containing 3% (v / v) of the lily tree extract of Formulation Example 4, respectively Was replaced with adenosine, a substance known to have an anti-wrinkle effect, and the cream of Comparative Formula 4 was used in the experiment.

The experiment group was divided into two categories for 20 experimenters (women aged 20 to 35 years). Example 4 was applied twice a day for 8 consecutive weeks. The skin elasticity improvement effect after the completion of the experiment was measured by using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months, respectively, and the experimental results are shown in Table 17 below. It is represented by the ΔR8 value of SEM 575. This ΔR8 value indicates the nature of the viscoelasticity of the skin.

Skin elasticity improvement effect Skin elasticity improvement effect (△ R8) Cream of Prescription Example 4 (Juliea Extract) 0.884 Cream (purified water) of comparative prescription example 3 0.268 Cream (adenosine) of comparative prescription example 4 0.864

As can be seen from the results of Table 17, the cream of Formulation Example 4 containing a lily extract as an active ingredient is about three times better skin elasticity improvement effect than the cream of Comparative Formulation Example 1 containing no lily extract It was shown that, compared with the cosmetic composition of Comparative Formulation Example 2 containing adenosine shows a similar skin elasticity improving effect, it can be seen that the cosmetic composition containing the lily extract of the present invention has an excellent skin elasticity improving effect.

Experimental Example 10 Sebum secretion inhibitory effect of the cosmetic containing the lily extract

In order to confirm the sebum secretion inhibitory effect of the cosmetic containing the lily extract of the present invention, the following experiment was performed by dividing three groups of 30 men and women with a large amount of sebum secretion.

 Experiment was prepared in the composition of the softening water containing the lily extract obtained in Example 1 in the composition of Formulation Example 1 of Table 9, wherein the lotion containing purified water instead of lily extract for comparison of sebum secretion inhibitory effect ( Comparative Prescription Example 1) Extract of white buckwheat extract known to have sebum secretion effect instead of lily extract (1.0% (w / v) white bran extract extracted with 70% ethanol and dissolved in 50% 1,3-butylene glycol) Experiment using the formulation of this containing lotion (Comparative Formulation Example 2). That is, in Group A, the flexible lotion of Formulation Example 1 containing a lily extract, Group B in Comparative Formulation 1, which does not contain a lily extract, and in Group C, the extract of Sesame Seeds is known to have sebum secretion effect. Comparative Comparative Example 2 contained was to be used every morning, evening after washing for 4 weeks. Sebum measurement was stabilized in a constant temperature and humidity room for 30 minutes after washing the face, and then sebum was measured on the forehead by using a sebumeter. The sebum amount was set to an initial value before using the flexible lotion for the test, and the change in sebum amount was measured once a week for 4 weeks while using the flexible lotion continuously. The change of sebum amount by the lily tree extract of the present invention was determined whether the sebum inhibitory effect compared to the initial sebum amount, the results are shown in Table 18.

Sebum secretion inhibitory effect division Sebum secretion (g / ㎠ / h) Initial value 2 weeks 4 Weeks Prescription Example 1 (70% Ethanol Extract of Lily of the Valley) 208.8 160.4 146.2 Comparative prescription example 1 (purified water) 210.6 200.4 198.2 Comparative Prescription 2 (white oyster extract) 206.8 172.6 150.6

As can be seen from the results of Table 18, it was confirmed that the sebum secretion amount of Formulation 1 treatment group to which the lily extract of the present invention is added as time passes. This result shows that the extract of the lily of the valley can be used as a cosmetic having a function of inhibiting pore dilatation by adjusting sebum secretion.

Experimental Example 11. Improvement of Skin Moisturizing Effect of Cosmetics Containing Lily Extract

In order to find out the effect of improving the skin moisturizing power of the cosmetic composition containing the lily of the valley of the present invention was carried out as follows.

It is divided into two groups of 20 people per trillion for 40 people in their 20s to 40s without skin diseases. Compared. That is, a lotion (prescription example 1) containing a lily extract of the present invention, a lotion (comparative example 1) containing purified water instead of a lily extract is applied twice a day for 1 month to the face and forearm to apply TEWA meter It was shown in Table 19 to evaluate the degree of improvement by measuring the skin moisturizing condition using.

Skin moisturizing effect improvement (device evaluation) division TEWL (g / h / m ² ) Initial value 2 weeks 4 Weeks Prescription Example 1 (70% Ethanol Extract of Lily of the Valley) 18.4 16.5 16.4 Comparative prescription example 1 (purified water) 18.6 18.2 18.3 Comparative Formulation Example 2 (white bran extract) 18.3 17.0 16.8

As shown in the results of the evaluation of the effect of improving skin moisturizing power by measuring the apparatus of Table 19, the cosmetic containing the lily extract of the present invention (prescription example 1) was added to the cosmetic (comparative prescription example 1) containing no lily extract. Compared to the skin moisturizing effect was very excellent. In addition, as a result of measuring the effect of improving the moisturizing power after 2 weeks and 4 weeks of use, similarly, using the cosmetics containing the lily extract of the present invention was confirmed that the skin moisturizing effect is improved and excellent.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (4)

Cosmetic composition for preventing skin aging, antioxidant, skin cell regeneration, skin wrinkle improvement, anti-inflammatory, sebum secretion or skin moisturizing containing lily extract as an active ingredient. The cosmetic composition according to claim 1, wherein the lily extract contains 0.00001 to 30.0% (w / w) based on the total weight of the cosmetic. The method of claim 1, wherein the extract of the lily tree is a lily, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, butyl acetate, chloroform, diethyl ether, Any one selected from a conventional extraction method, an ultrasonic extraction method, a supercritical extraction method, a fermentation method and an extraction method using a solvent extraction method using an extraction solvent selected from the group consisting of dichloromethane, hexane and mixtures thereof. It is obtained by the method, The cosmetic composition characterized by the above-mentioned. The cosmetic composition of claim 1 is applied to human skin to improve skin wrinkles, antioxidants, regenerate skin cells, alleviate cell irritation, inhibit skin damage caused by ultraviolet light, anti-inflammatory, prevent skin aging, improve skin elasticity, inhibit sebum secretion and moisturize skin. Cosmetic method, characterized in that to obtain any one of the skin condition improvement effect selected from.