KR20090055956A - Cosmetic composition for skin-aging protection and wrinkle improvement comprising the extract of lindera syrychnifolia as active ingredient - Google Patents

Abstract

A cosmetic composition containing Lindera syrychnifolia extract is provided to have the effects of MMP-1(matrix metalo-proteinase 1) generation inhibition, collagen synthesis enhancement, skin irritation relief, and anti-inflammation. A cosmetic composition for antiaging and antiwrinkle effects comprises 0.001-30.0% by weight of Lindera syrychnifolia extract as an active ingredient. The Lindera syrychnifolia extract is obtained by isolating leaf, flower, root, stem, seeds of the Lindera syrychnifolia using a solvent extraction method with water, hydrous or anhydrous low alcohol of 1-4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate or 1,3-butylene glycol, supercritical extraction or sonication extraction. The Lindera syrychnifolia extract is obtained by filtering at room temperature and compression-extraction or freeze-drying. The cosmetic composition is used in a form of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder type foundation, emulsion type foundation, wax type foundation, or spray type foundation.

Description

Cosmetic composition for preventing skin aging and improving skin wrinkles containing ointment extract as active ingredient {Cosmetic Composition for Skin-aging Protection and Wrinkle Improvement Comprising the Extract of Lindera syrychnifolia as Active Ingredient}

The present invention relates to a cosmetic composition containing the ointment as an active ingredient, and more particularly, the present invention relates to a skin composition for preventing skin aging and improving wrinkles containing the ointment extract as an active ingredient.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into internal aging and photo-aging, and research on this has been actively conducted. Free radicals may be activated by UV rays, stress, disease conditions, environmental factors, wounds, and age. If this condition is exacerbated, it destroys antioxidant defenses in vivo and damages cells and tissues. And aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins is caused by severe cuts in the connective tissues of collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, and so on, resulting in severe over-inflammatory reaction and skin elasticity. If this worsens, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

Therefore, it protects the cell membrane by eliminating the free radicals caused by metabolic processes of the body, irradiation by UV irradiation and inflammatory reactions, and the already damaged cells should be regenerated by active metabolism to proliferate the cells. You can recover quickly and maintain healthy skin. Aging involves not only free radicals, but also an enzyme called matrix metalloprotease (MMP) .In vivo, the synthesis and degradation of extracellular substrates such as collagen are properly regulated, but their synthesis decreases as aging progresses. Expression of matrix metalloproteinase (MMP), which is an enzyme that degrades, is promoted, thereby decreasing the elasticity of the skin and forming wrinkles. Ultraviolet irradiation also activates these degrading enzymes. Therefore, there is a need for the development of a substance capable of regulating MMP expression that inhibits activation or inhibiting its activity in cells. Until now, most of the raw materials used as materials for cosmetics simply inhibited enzyme activity. Therefore, we tried to regulate the expression and activity of MMP expression induced by intracellular natural aging and photoaging.

Meanwhile, various chemical substances are used as the cosmetic composition, but recently, cosmetics using natural products have been developed to reduce skin irritation caused by such chemical substances. Natural materials have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing.

The present inventors have studied the possibility of applying to cosmetics in various natural products which have not been known until now, and found that the oak extract, which is a plant of Camphoraceae, has an excellent effect on skin antioxidant effect, collagen synthesis effect, and skin wrinkle relief effect. The invention has been completed.

Therefore, it is an object of the present invention to provide a cosmetic composition for preventing skin aging containing a medicinal extract as an active ingredient.

Another object of the present invention to provide a cosmetic composition for improving skin wrinkles containing oak extract as an active ingredient.

Another object of the present invention is to provide a cosmetic method comprising applying a cosmetic composition containing the oak extract to the human skin.

Other objects and advantages of the invention will be apparent from the following examples and claims.

The present invention relates to a cosmetic composition containing the oak extract as an active ingredient, and more particularly, the present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles containing the oak extract as an active ingredient. The potent extract of the present invention has an excellent anti-aging effect such as antioxidant effect, MMP-1 production inhibitory effect, MMP-1 production inhibitory effect by UV irradiation, collagen synthesis promotion effect, and skin irritation effect and anti-inflammatory effect after UV irradiation. Indicated. In addition, the cosmetic composition containing the oak extract of the present invention is excellent in skin elasticity improving effect and moisturizing effect.

Lindera aggregate ; Lindera syrychnifolia ) is a plant of the Camphoraceae, with oval or egg-shaped leaves alternately attached, and small flowers of yellow grass color. It grows in southern China (Fujian, Zhejiang, Jiangsu, Taiwan) and has 0.1 ~ 0.2% essential oil in its roots. Essential oils include terpene compounds such as lindenitol (lindenenol, linestene, and linderalactone), and the dried root is mainly used for medicinal purposes. I'm sick and cold, I eat up, vomiting, bladder cold and pee frequently, used as a intermediate for cerebral hemorrhage, crustal paralysis, head pain [[Medicinal ingredients and use of herbs, science, encyclopedia publishing company / January Seokje / Choi Okja / 1994] In addition, medicinal herbs circulate the body, warm up the stomach, and use it for polyuria, digestive disorders, diarrhea, moonshine, etc. There are 0.1 ~ 0.2% essential oils in the roots, including Lindenol and Linde. It contains terpene compounds such as nin, Lindestrene, Linderan, Linderoxide, etc. It helps to relieve pain It is effective when vomiting due to indigestion and frequent urination. Yuhan Culture four].

As used herein, the term “oak extract” means that it is obtained by extracting from various organs or parts of the oak (eg, leaves, flowers, roots, stems, branches, bark and seeds, etc.), preferably leaves, stems, branches , Bark or rhizome, more preferably leaves, branches or rhizome, most preferably means extracts obtained from rhizome.

The oak extract of the present invention can be prepared according to conventional methods known in the art, i.e., under the conditions of ordinary temperature and pressure, using conventional solvents, preferably purified water, methanol, ethanol, glycerin, Extracted using at least one extraction solvent selected from the group consisting of ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane, more preferably ethanol, 70% ethanol or Extracted using water, most preferably 70% ethanol.

On the other hand, it is apparent to those skilled in the art that the ointment extract of the present invention can obtain an extract having substantially the same effect using not only the above-described extraction solvent but also other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with constant molecular weight cut-off values, various chromatography (size, charge, hydrophobicity or affinity) The active fraction obtained through various purification methods additionally carried out, such as separation by sex)) is also included in the extract of the present invention.

In another aspect, the present invention provides a composition for external application for skin, characterized in that the extract is obtained by cold condensation at room temperature, heat filtration, and further obtained by concentrating the solvent under reduced pressure or lyophilization.

In addition, the oak extract of the present invention is contained 0.001-30.0% by weight, preferably 0.1-10.0% by weight based on the total cosmetic composition. When the content of the extract is less than 0.001% by weight, the antioxidant, anti-aging and skin wrinkle improvement effects are extremely weak. When the content of the extract is more than 30.0% by weight, the effect of increasing the content is insignificant. It is not economical either.

According to the present invention, the oak extract has an antioxidant effect (Experimental Examples 1 and 2), MMP-1 production inhibitory effect (Experimental Example 3), MMP-1 production inhibitory effect by ultraviolet light (Experimental Example 4), collagen synthesis promoting effect ( Experimental Example 5), excellent cytotoxicity and anti-inflammatory effect (ultraviolet) (experimental examples 6, 7), skin elasticity improvement (experimental example 8) and moisturizing effect (experimental example 9).

Therefore, the cosmetic composition of the present invention containing the oak extract having various functions as an active ingredient has an antioxidant effect, an anti-aging effect, a wrinkle improvement and a moisturizing effect.

The ointment extract of the present invention can achieve its effect by topical application or spraying on the skin. Therefore, according to one preferred aspect of the present invention, the composition of the present invention may be prepared in the form of cosmetic compositions such as creams, lotions and softening water or pharmaceutical compositions such as sprays and ointments.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing It may be formulated as, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystalline as carrier components Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention provides a cosmetic method characterized in that the cosmetic composition containing the oak extract of the present invention is applied to human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic method including the oak extract of the present invention is applied to a human skin, a cosmetic method may be used to obtain anti-aging, wrinkle improvement and moisturizing effects of the skin.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1 Preparation of Ojami Extract

The root diameter of the thinly-sprayed ointment was refluxed three times for 5 hours with an aqueous 70% (V / V) ethanol solution, and the mixture was cooled and then filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. The extract was prepared by dispersing / dissolving in a 50% butylene glycol solution under reduced pressure and freeze-dried 1%.

Experimental Example 1 Experiment of Measuring Antioxidant Effect Using NBT Method

In order to confirm the antioxidant effect of the oak extract obtained in Preparation Example 1, a comparative sample of other antioxidants, such as retinol and BHT (Butylated Hydroxytoluene), was tested under laboratory conditions. Was measured.

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by the NBT method, and the effect of the test substance removing the active oxygen, that is, the active oxygen scavenging effect, is evaluated. Free radicals are produced by xanthine and xanthine oxidase. This active oxygen reacts with Nitro Blue Tetrazolium (NBT) to measure the active oxygen scavenging rate by measuring the blue color produced by this at a wavelength of 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to Bayer's bottle and add 0.1ml of sample solution to it and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

④ For the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

⑤ Also, the blank of the sample solution is operated in the same manner using distilled water instead of 6 to measure the absorbance Bo.

The reactive oxygen scavenging ratio was calculated by Equation 1, and the results are shown in Table 1.

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction when enzyme is not added to sample solution

Bo: Absorbance at 560 nm before reaction when enzyme is not added

Sample Name Treatment concentration (%) Antioxidative effect(%) Ojami extract (Manufacturing example 1) 0.01 94.3 Retinol 0.01 93.1 BHT 0.01 90.2

As shown in the results of Table 1, both the ointment extract, retinol and BHT of Preparation Example 1 was able to confirm an excellent antioxidant effect at a concentration of 0.01%, and the ointment extract of the present invention is much better than the known retinol and BHT. It was confirmed that it has excellent antioxidant power.

Experimental Example 2 Experiment of Measuring Antioxidant Effect Using DPPH Method

In this Experimental Example, to measure the antioxidant effect of the oak extract obtained in Preparation Example 1, the antioxidant activity such as vitamin C was compared in the laboratory conditions and the antioxidant activity was measured using the DPPH method.

The DPPH method measures the antioxidant activity by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The free radical scavenging rate is measured at a wavelength of 560 nm by comparing the degree to which the DPPH is reduced by the test substance to reduce the absorbance.

As a reagent used, 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) 0.1 mM solution was dissolved in methanol to make 100 ml.

As a measuring method,

① Add 0.15ml of sample solution to 0.15ml of 0.1mM DPPH solution in 96-well plate, stir rapidly, and incubate for 10 minutes at 25 ℃.

(2) Then measure the absorbance St at 560nm.

③ In the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

④ Again, the blank of the sample solution was operated in the same manner using methanol instead of the 0.1 mM DPPH solution to measure the absorbance Bo.

The result of the effect was calculated by Equation 2, the results are shown in Table 2.

St: absorbance at 560 nm after free radical scavenging of sample solution

Bt: absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction without free radicals in sample solution

Bo: absorbance at 560 nm before reaction without free radicals in blank test solution

Sample Name Treatment concentration (ppm) Antioxidative effect(%) Ojami Extract (Manufacturing Example 1) 10 31.38 Ojami Extract (Manufacturing Example 1) 20 56.16 Ojami Extract (Manufacturing Example 1) 50 91.16 Ojami Extract (Manufacturing Example 1) 100 92.78 Ojami Extract (Manufacturing Example 1) 200 93.23 Ojami Extract (Manufacturing Example 1) 400 94.14 Ojami Extract (Manufacturing Example 1) 800 94.85 Vitamin c 0.01 89.1

As shown in the results of Table 2, it was confirmed that the oak extract of the present invention has a much better free radical scavenging ratio than vitamin C at a concentration of 50 ppm.

Experimental Example 3. Effect of Inhibiting MMP-1 Production

Fibroblasts (Korean Cell Line Bank, South Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. After 24 hours of incubation, the bacterium extract of Preparation Example 1 was further incubated for 48 hours in a serum-free DMEM medium to which the final concentration was added at a final concentration of 100 μg / ml and a serum-free DMEM medium not containing the bacterium extract as a control. After incubation, the supernatant of each well was collected and the amount of ng / ml newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA), and MMP-1 production was performed according to Equation 3 below. Inhibition rate was calculated and the results are shown in Table 3. At this time, TGF-β (10 ng / ml, Roche, United States) was used as a positive control of MMP-1 production inhibition rate.

Inhibition Rate of MMP-1 Production by Ojak Extract (%) Sample Name Treatment concentration (ppm) MMP-1 Production Inhibition Rate (%) Ojami Extract (Manufacturing Example 1) 10 10.3 Ojami Extract (Manufacturing Example 1) 20 30.5 Ojami Extract (Manufacturing Example 1) 50 60.3 Ojami Extract (Manufacturing Example 1) 100 68.5 Ojami Extract (Manufacturing Example 1) 200 73.5 Ojami Extract (Manufacturing Example 1) 400 80.1 Ojami Extract (Manufacturing Example 1) 800 89.3 TGF-β 0.01 68.5

As shown in the results of Table 3, the oak extract of the present invention showed a much higher MMP-1 production inhibition rate than TGF-β at a very low concentration.

Experimental Example 4. Inhibition of MMP-1 Production by Induction of Ultraviolet Radix Extract

In general, MMP-1 is known to increase its amount by ultraviolet light. Therefore, the experiment was conducted to determine whether the oak extract of the present invention inhibits the amount of MMP-1, which is increased by UV light. The experiment was performed in the same manner as in Experiment 3 except that a certain amount of UVA was treated before sample treatment. The ointment extract used in this experiment was used as the extract of Preparation Example 1, but the results of this experiment are not limited thereto. MMP-1 production inhibition rate was calculated according to Equation 3, the results are shown in Table 4 below.

Inhibition Rate of MMP-1 Formation by Opaque Extracts after UV Induction (%) Sample Name Treatment concentration (ppm) MMP-1 Production Inhibition Rate (%) Ojami Extract (Manufacturing Example 1) 10 13.1 Ojami Extract (Manufacturing Example 1) 20 33.6 Ojami Extract (Manufacturing Example 1) 50 63.8 Ojami Extract (Manufacturing Example 1) 100 68.3 Ojami Extract (Manufacturing Example 1) 200 75.9 Ojami Extract (Manufacturing Example 1) 400 87.4 Ojami Extract (Manufacturing Example 1) 800 89.2 TGF-β 0.01 75.3

As shown in the results of Table 4, the inhibition rate of MMP-1 production after UV induction of the oak extract of the present invention was better than TGF-β.

Experimental Example 5. Collagen Synthesis Enhancement Effect of Ojak Extract

Fibroblasts, human normal epithelial cells, were seeded in 48-well microplates at 1 × 10 ⁶ cells per well and incubated in DMEM medium for 24 hours. Serum-free DMEM medium (control group) and the serum-free DMEM medium (control group) containing no oak extract were further cultured for 48 hours. After incubation, the supernatant of each well was collected and measured in ng / ml by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a collagen kit (Takara, Japan), and the amount of collagen synthesized therefrom was measured. It was. TGF-β and retinol were used as positive controls in this experiment. Collagen biosynthesis increase rate was calculated according to the following equation (4), the results are shown in Table 5.

Collagen Synthesis Enhancement Effects of Oja Extracts Sample Name Treatment concentration (ppm) MMP-1 Production Inhibition Rate (%) Ojami Extract (Manufacturing Example 1) 10 - Ojami Extract (Manufacturing Example 1) 20 10.3 Ojami Extract (Manufacturing Example 1) 50 30.3 Ojami Extract (Manufacturing Example 1) 100 45.5 Ojami Extract (Manufacturing Example 1) 200 63.7 Ojami Extract (Manufacturing Example 1) 400 75.7 Ojami Extract (Manufacturing Example 1) 800 80.3 Retinol 400 68.4 TGF-β 200 nM 70.3

As shown in the results of Table 5, it was confirmed that the collagen synthesis enhancing effect of the oak extract of the present invention has an excellent effect when compared with TGF-β and retinol.

Example 6 Cytotoxicity Reduction Effect by UV Irradiation

This example was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the oak extract obtained in Preparation Example 1. Fibroblasts (fibroblasts) were placed in a 24-well test plate each ⁵ × ¹ × ¹⁰ attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B15W, Sankyo Dennki, Japan), and then PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. After treatment with the oak extract to be evaluated incubated for 24 hours. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed, and 500 μl of isopropanol-HCl (0.04N) was added thereto. After shaking for 5 minutes, the cells were lysed and 100 μl of the supernatant was transferred to a 96-well test plate, and the absorbance was measured at 565 nm using a microplate reader. The cell viability (%) was measured by the following Equation 5, and the cytotoxicity relaxation rate by ultraviolet rays was calculated by the following equation (6). The results are shown in Table 6.

Bo: 565 nm absorbance of wells that only developed cell culture media

Bt: 565 nm absorbance of the wells that developed the colorless reaction wells

St: 565 nm absorbance of the wells that reacted the sample treated wells

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: cell viability of wells that were not irradiated with UV light

St: Cell survival rate of wells treated with UV

Cytotoxic alleviation rate (%) Sample Name Treatment concentration Cytotoxic alleviation rate (%) Ointment extract 0.010% 65 Ointment extract 0.020% 73

As shown in the results of Table 6, the oak extract of the present invention suppresses cytotoxicity caused by ultraviolet rays at 65% at 0.010% concentration, and inhibits 73% at 0.020%, effectively protecting cytotoxicity caused by ultraviolet light. Could know.

Experimental Example 8: Inhibitory Effect of Inflammatory Cytokine Expression by Ultraviolet Irradiation

This Example is to evaluate the inhibitory effect of inflammatory cytokine expression expressed by UV irradiation of the oak extract obtained in Preparation Example 1 Fibroblasts isolated from human epidermal tissue (Fbroblast) in a 24-well test plate 5X10 ⁴ One by one and attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki Co., Japan), and then PBS was removed and cell culture medium (FMEM was not added to DMEM). Medium) 350 μl was added. After treatment with the oak extract to be evaluated incubated for 5 hours. 150 μl of the culture supernatant was taken to quantify IL-1α to determine the inhibitory effect of inflammatory cytokine expression of the oak extract. The amount of IL-1α was quantified using Enzyme-linked Immunosorbent Assay, and the production rate of IL-1α was calculated by Equation 7. The results are shown in Table 7.

Bo: IL-1α production in wells without UV irradiation

Bt: IL-1α production in wells that were not irradiated with UV light

St: IL-1α production amount of wells that were irradiated with UV light

Sample Name Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Ointment extract 0.010% 63% Ointment extract 0.020% 72%

As shown in the results of Table 7, the oak extract of the present invention inhibits the production of inflammatory cytokines, IL-1α, by 58% at a concentration of 0.010%, and inhibits 72% at 0.020%, resulting in a low treatment. It can be seen that the concentration effectively protects against inflammation caused by ultraviolet rays.

Hereinafter, the formulation was used for the oak extract prepared from Preparation Example 1 of the present invention, but is not limited thereto.

Formulation Example 1 Flexible Lotion (Skin)

Formulation examples of the flexible lotion (skin) in the cosmetic composition containing the ointment extract of the present invention is as follows:

ingredient Content (% by weight) Ojak extract of Preparation Example 1 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2. Nutrients (Milk Lotion)

Formulation examples of nutrient cosmetics (milk lotion) in the cosmetic composition containing the oak extract of the present invention is as follows:

ingredient Content (% by weight) Ojak extract of Preparation Example 1 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3. Nutrition Cream

Examples of the formulation of the nourishing cream in the cosmetic composition containing the oak extract of the present invention are as follows:

ingredient Example 1 Comparative Example 1 Ointment extract 3.0 - Lipophilic monostearate 2.0 2.0 Cetearyl Alcohol 2.2 2.2 Stearic acid 1.5 1.5 Beeswax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan stearate 0.6 0.6 Cured Vegetable Oil 1.0 1.0 Squalane 3.0 3.0 Mineral oil 5.0 5.0 Trioctanoine 5.0 5.0 Dimethicone 1.0 1.0 Sodium magnesium silicate 0.1 0.1 glycerin 5.0 5.0 Betaine 3.0 3.0 Triethanolamine 1.0 1.0 Sodium hyaluronate 4.0 4.0 Preservative, fragrance, coloring a very small amount a very small amount Distilled water Remaining amount Remaining amount Sum 100 100

* The content units in the table are by weight.

Formulation Example 4 Massage Cream

Examples of the formulation of the massage cream in the cosmetic containing the oak extract of the present invention are as follows:

ingredient Content (% by weight) Ojak extract of Preparation Example 1              0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5 Pack

Examples of the formulation of the pack in the cosmetic containing the oak extract of the present invention are as follows:

ingredient Content (% by weight)  Ojak extract of Preparation Example 1 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

Experimental Example 9. Improvement of skin elasticity of cosmetics containing ointment extract

The skin elasticity improvement effect of the cosmetics of the present invention was evaluated through a practical use test. The following experiment was performed using the nutrition cream (Example 1) of the formulation example 3 containing the oak extract of this invention, and the comparative example 1 of the formulation example 3 containing no oak extract. 20 experimenters (20-35 year old female) were applied to the right side of the face cream of Example 1 and the left side of the face of the cream of Comparative Example 1 was applied twice a day for three consecutive months. The skin elasticity improvement effect after the completion of the experiment was measured using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months. The experimental results are described in Table 13 below as the ΔR8 value of the cutometer SEM 575, where the R8 value represents the nature of the viscoelasticity of the skin.

Experimental product Skin elasticity effect (△ R8) Example 1 0.26 Comparative Example 1 0.11

 As shown in the results of Table 13, it can be seen that Example 1 containing the oak extract of the present invention is about two times better skin elasticity improvement effect than Comparative Example 1 does not contain the oak extract.

Experimental Example 9. Moisturizing effect of cosmetics containing ointment extract

In order to evaluate the moisturizing power of the cosmetics prepared in Formulation Example 3 (Example 1 and Comparative Example 1), the moisture retention capacity of the skin to which each cosmetic was applied was measured as follows.

In the constant temperature and humidity room of 22 ° C. and 45% RH, each of the cosmetics of Example 1 and Comparative Example 1 was divided into half of 24 test subjects, and the product of Example 1 was applied to one side and the product of Comparative Example 1 to the other. The water content was measured 4 weeks later (twice / day).

As a measuring device, a moisture content measuring device (Corneometer CM825, Courage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 14 below.

Moisture Content (mg / cm ² / hr) Use week Example 1 Comparative Example 1 0 Week 23.61 ± 9.34 23.44 ± 11.36 4 Week 34.61 ± 7.09 27.38 ± 5.58 4 Week-0 Week 11.00 ± 2.25 3.94 ± 5.78

As shown in the results of Table 14, the cosmetic composition containing the oak extract of the present invention (Example 1) was significantly superior to the skin moisturizing effect compared to the cosmetic composition (Comparative Example 1) containing no oak extract.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (6)

Cosmetic composition for preventing skin aging containing the oak extract as an active ingredient. Cosmetic composition for improving skin wrinkles containing the oak extract as an active ingredient. The cosmetic composition according to claim 1 or 2, wherein the oak extract is contained in an amount of 0.001-30.0% by weight based on the whole composition. The group of claim 1 or 2, wherein the oak extract comprises (a) a group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butylacetate and 1,3-butylene glycol Cosmetic composition, characterized in that extracted using the solvent extraction method, (b) supercritical extraction method or (c) ultrasonic extraction method using an extraction solvent selected from. 3. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation And a formulation selected from the group consisting of sprays. The cosmetic method according to any one of claims 1 to 5, wherein the cosmetic composition is applied to human skin.