KR101176522B1 - Cosmetic composition comprising the extract of Cypripedium macranthum as active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a cypripedium macranthum (Cypripedium macranthum) extract as an active ingredient, characterized in that it comprises 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition, the present invention According to the present invention, the lucky bag extract has not only antioxidant effect but also fibroblast proliferation effect, MMP-1 production inhibitory effect, UV-induced MMP-1 production inhibitory effect, collagen synthesis enhancement effect, melanin production inhibitory effect, UV irradiation cell Toxicity mitigation effect, inflammatory cytokine expression suppression effect by ultraviolet irradiation, skin elasticity improvement effect, wrinkle improvement effect and moisturizing effect can provide a variety of functional cosmetics.

With lucky bag, antioxidant, anti-aging, whitening, wrinkle, moisture, skin irritation relief, cosmetics

Description

Cosmetic composition comprising the extract as an active ingredient bokbok bag {Cosmetic composition comprising the extract of Cypripedium macranthum as active Ingredient}

The present invention relates to a cosmetic composition containing an extract of the lucky bag as an active ingredient.

Human skin undergoes various physical and chemical changes during the aging process. The causes are largely divided into intrinsic aging and photo-aging, and research on this has been actively conducted. UV rays, stress, disease conditions, environmental factors, wounds, and free radicals may be caused by aging, which intensifies the body's ability to destroy antioxidant defenses and damage cells and tissues. And aging.

More specifically, lipids, proteins, polysaccharides, nucleic acids, and the like, which are major constituents of skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

In particular, the oxidation of proteins is caused by severe cuts in the skin's connective tissues such as collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, resulting in severe over-inflammatory reactions and skin elasticity. If this becomes worse, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

Therefore, it is necessary to protect the cell membrane by eliminating free radicals generated by metabolic processes of the body, ultraviolet rays, and inflammatory reactions.Also, damaged cells must be regenerated by active metabolism to proliferate the cells. The skin can recover quickly and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. In other words, the synthesis and degradation of extracellular matrix such as collagen in vivo is properly controlled, but collagen synthesis decreases as aging progresses, and the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, promotes the skin. Elasticity is reduced and wrinkles are formed. In addition, such degradation enzymes may be activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to control the amount and activity of MMP expression induced by intracellular natural aging and photoaging.

On the other hand, skin changes due to pigment deposition occur. Factors that determine skin color include basic or partial pigmentation, such as tanning due to blemishes, freckles and ultraviolet exposure, except for race, region, gender and age differences, as well as the distribution of acne, scars and keratin. And blood circulation, stress, and health. Among the above factors, the most important factor is pigmentation.

Pigments that affect skin color include melanin, melanoids, carotene, and hemoglobin, the most important of which is melanin, the most important factor affecting the biosynthesis of melanin is the amount of ultraviolet rays and hormones in the body.

Melanin plays a major role in preventing or damaging the skin from ultraviolet rays by absorbing or scattering ultraviolet rays. There is no special maximum absorption wavelength and it absorbs light in all areas. In addition, the function of removing active oxygen species is excellent, but sometimes melanin itself generates free radicals, and other substances are reduced or oxidized by catechol or quinone in melanin structure, and melanin itself shows the properties of free radicals. Pray.

The biosynthesis of melanin begins with the conversion of tyrosine, an amino acid, into melanosomes of melanocyte-forming melanosomes by tyrosinase and conversion to dihydroxy phenylalanine, followed by a series of oxidation processes to form pheomelanin, eumelanin.

This biosynthesis process is carried out in a special type of brown cells in organelles called melanosomes. Melanosomes, including melanin granules, move from the periphery of the nucleus to the dendritic end of the dendritic projections and into the cytoplasm by the action of keratinocytes. Accumulate around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to the surrounding keratinocytes are partially affected by hormones and ultraviolet rays, and primarily by genetic influences. In addition, it is known that interferon, prostaglandin, histamine, and other metal ions such as cytokines, copper, zinc and iron, which are intracellular regulators involved in the expression of tyrosinase and the synthesis and transfer of melanin.

(Japanese Patent Laid-open No. 4-9320), hydroquinone (Japanese Patent Laid-Open No. 192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Activity, and is used as a whitening cosmetic. However, since it is low in stability in cosmetic formulations, its use is restricted due to decomposition and coloring, generation of odor, efficacy at a living body level, unclear effect and safety problem. The above substances have been shown to have a tyrosinase inhibitory effect, but the effect is low in experiments similar to the actual biological level. Therefore, the evaluation of the inhibitory effect by melanoma cell culture similar to the biological level is required. Hydroquinone is also defined as a carcinogenic substance and its use in cosmetics is prohibited or restricted. Kojic acid and ascorbic acid are very unstable substances. When a cosmetic containing a small amount of the above components is kept at room temperature for several weeks, browning occurs.

In order to solve these various problems, cosmetics using natural products have recently been developed to reduce skin irritation caused by various chemicals. Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has increased recently, the development value of cosmetics as an ingredient is increasing.

Therefore, the present inventors have studied the possibility of applying to cosmetics in a variety of natural products that have not been known so far, by selecting a lucky bag and extract from it, the skin antioxidant effect, fibroblast proliferation effect, MMP-1 production inhibition Effect, inhibiting MMP-1 production by UV induction, enhancing collagen synthesis, inhibiting melanin production, reducing cytotoxicity by UV irradiation, inhibiting inflammatory cytokine expression by UV irradiation, improving skin elasticity, wrinkle improvement As a result of measuring the effect and the moisturizing effect, it was found that the efficacy as a cosmetic can be expected because it is very excellent.

The purpose of the present invention is to contain the extract of the lucky bag, skin antioxidant effect, fibroblast proliferation effect, MMP-1 production inhibitory effect, UV-induced MMP-1 production inhibitory effect, collagen synthesis enhancement effect, skin elasticity improving effect, skin It is to provide a cosmetic composition exhibiting an excellent anti-aging effect, such as wrinkle improvement effect.

In addition, the object of the present invention is the application of the extract of the lucky bag to the skin cosmetics, melanin production inhibitory effect (whitening effect), cytotoxic alleviation effect, such as skin irritation effect, inflammatory cytokine expression inhibitory effect (skin damage inhibitory effect) and It is to provide a cosmetic composition exhibiting a moisturizing effect.

Another object of the present invention to provide a cosmetic method using the cosmetic composition containing the extract of the lucky bag.

The object of the present invention as described above is achieved by a cosmetic composition containing an extract of the lucky bag as an active ingredient.

Preferably, the cosmetic composition is characterized in that for antioxidant.

In addition, preferably, the cosmetic composition is characterized in that for preventing skin aging.

In addition, the cosmetic composition is characterized in that for improving skin elasticity.

In addition, the cosmetic composition is characterized in that for improving the skin wrinkles.

In addition, the cosmetic composition is characterized in that the skin for whitening.

In addition, the cosmetic composition is characterized in that for inhibiting skin damage.

In addition, preferably, the cosmetic composition is characterized in that for reducing skin irritation.

In addition, the cosmetic composition is characterized in that for moisturizing the skin.

In addition, preferably, the lucky bag extract is characterized in that it contains 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition.

Preferably, the lucky bag extract is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform , Butyl acetate, diethyl ether, dichloromethane, hexane and the extract is extracted using a solvent selected from the group consisting of, or is characterized in that it is extracted by supercritical extraction or ultrasonic extraction.

Also preferably, the cosmetic composition eliminates free radicals, inhibits MMP-1 production and inhibits MMP-1 production by ultraviolet irradiation, enhances collagen synthesis, inhibits melanin biosynthesis, and Alleviates cytotoxicity and inhibits the expression of inflammatory cytokines by UV irradiation, antioxidation effect, skin elasticity improvement effect, skin wrinkle improvement effect, anti-aging effect, whitening effect, cytotoxic effect effect by UV irradiation, skin damage It is characterized by exhibiting an inhibitory effect, a stimulating alleviating effect and a moisturizing effect.

Also, preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray It is characterized by having a formulation selected from the group consisting of.

Another object of the present invention is to apply the cosmetic composition to the human skin, the antioxidant effect, anti-aging effect, skin elasticity improvement effect, skin wrinkle improvement effect, skin whitening effect, skin damage inhibition effect by ultraviolet light, irritation relief It is achieved by a cosmetic method characterized in that the effect or skin moisturizing effect is obtained.

Lucky bag extract according to the present invention has a skin antioxidant effect (Experimental Example 1, 2), fibroblast proliferation effect (Experimental Example 3), MMP-1 production inhibitory effect (Experimental Example 4), MMP-1 production by UV induction Inhibitory effect (Experimental Example 5), collagen synthesis enhancement effect (Experimental Example 6), melanin production inhibitory effect (Experimental Example 7), cytotoxicity relaxing effect (Experimental Example 8), inflammatory cytokine expression inhibitory effect (Experimental Example 9), It has skin elasticity improving effect (Experimental Example 10), skin wrinkle improvement effect (Experimental Example 11) and moisturizing effect (Experimental Example 12).

In more detail, the lucky bag extract of the present invention has an anti-aging effect such as antioxidant effect, fibroblast proliferation effect, MMP-1 production inhibitory effect and MMP-1 production inhibitory effect by UV rays, elasticity improvement effect and after UV irradiation It has an effect of alleviating the skin irritation caused, and has a whitening effect such as melanin production inhibitory effect, which is a substance causing blemishes, freckles and skin pigmentation.

Therefore, the cosmetic composition comprising the extract of the lucky bag according to the present invention, the anti-oxidant effect, the elasticity improvement effect, the whitening effect and alleviate the cytotoxicity by ultraviolet irradiation, and inhibit the expression of inflammatory cytokines by ultraviolet irradiation, excellent skin damage It can be seen that it has an inhibitory effect, a stimulating effect and a moisturizing effect.

The present invention is an anti-oxidation, skin aging prevention, skin elasticity improvement, skin wrinkle improvement, skin whitening, skin damage suppression by ultraviolet rays, skin irritation and skin moisturizing containing the extract as an active ingredient It relates to a cosmetic composition for.

Cypripedium macranthum ( Cypripedium macranthum ) used as an active ingredient in the cosmetic composition of the present invention is also called orchid family dogbyeol alan, lupine flower, a flower of the flowering period and blooms in May-June. The growing environment is often found in halftones and grows wild in Jirisan where Southern and Northern Districts meet. In the private sector, the name of the herbal medicine called "Ok Gong Chil" is used as a medicinal herb including roots.

In the present invention, the lucky bag extract means that obtained by extracting from various organs or parts (for example, leaves, flowers, roots, stems, branches, bark and seeds, etc.) of the lucky bag, preferably leaves, stems , An extract obtained from an eggplant, a bark or a root, more preferably an extract obtained from a leaf, an eggplant or a root, and most preferably an extract obtained from a leaf.

In addition, the bag extract of the present invention can be prepared according to a conventional method known in the art, that is, using a conventional solvent, under the conditions of conventional temperature and pressure, preferably (a) water Anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, Solvent extraction using a solvent selected from the group consisting of dichloromethane, chloroform, hexane and mixtures thereof (b) extraction using supercritical extraction by reduced pressure and high temperature with carbon dioxide or (c) ultrasonic extraction.

In the present invention, a solvent extraction method using an extraction solvent is preferably used.

Lucky bag extract in the present invention is a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butyl It can be obtained using an extraction solvent selected from the group consisting of len glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof, preferably ethanol, 70% ( v / v) obtained using an ethanol aqueous solution or water, most preferably obtained using 70% (v / v) ethanol.

On the other hand, it is apparent to those skilled in the art that the lucky bag extract of the present invention can be obtained extracts having substantially the same effect using not only the above-described extraction solvent but also other extraction solvents.

In addition, the extract of the lucky bag of the present invention includes not only the extract by the above-described extraction solvent, but also the extract through a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with constant molecular weight cut-off values, various chromatography (size, charge, hydrophobicity or affinity) The active fraction obtained through various purification methods additionally carried out, such as separation by sex), is also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cold condensation at room temperature, heat filtration, and further obtained by concentrating the solvent under reduced pressure or lyophilization.

The decompression by carbon dioxide and the supercritical extraction by high temperature mean supercritical fluid extraction. Generally, the supercritical fluid is a liquid having a gas when the gas reaches a critical point under high temperature and high pressure. It is gaseous, chemically similar in polarity to nonpolar solvents, and because of its properties, supercritical fluids are used for extraction of fat-soluble substances (J. Chromatogr. A. 1998; 479: 200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gaseous properties as the pressure and temperature reach a critical point through the operation of a supercritical fluid device, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substance contained in the sample is extracted from the supercritical carbon dioxide.

When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using a supercritical carbon dioxide or a mixed fluid in which a co-solvent is added to carbon dioxide.

The cosolvent may be used one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether.

Most of the extracted samples contain carbon dioxide. Since carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method may be used as a cosmetic composition, and the cosolvent may be removed by a reduced pressure evaporator.

In addition, the ultrasonic extraction method is an extraction method using the energy generated by the ultrasonic vibration, the ultrasonic wave can destroy the insoluble solvent contained in the sample in the water-soluble solvent, the reactants located around due to the high local temperature generated Since the kinetic energy of the particles is increased, sufficient energy required for the reaction is obtained, and the effect of ultrasonic energy is induced to increase the pressure by increasing the mixing effect of the substance and the solvent contained in the sample, thereby increasing the extraction efficiency.

As the extraction solvent that can be used in the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used.

The extracted sample may be vacuum filtered to recover the filtrate and then removed by a vacuum evaporator, and the extract may be obtained through a conventional extract preparation method of lyophilization.

The cosmetic composition of the present invention eliminates free radicals and free radicals, promotes fibroblast proliferation, inhibits the production of MMP-1 and inhibits the production of MMP-1 overproduced by ultraviolet light, promotes biosynthesis of collagen, An effective amount of clothing that inhibits melanin production, relieves cytotoxicity caused by ultraviolet light, inhibits the expression of inflammatory cytokines expressed by ultraviolet light, improves skin elasticity and wrinkles, and is effective for moisturizing. Pouchlan is characterized in that it comprises an extract.

According to the present invention, the lucky bag extract contains 0.001 to 30.0% by weight relative to the total weight of the cosmetic composition, more preferably, the lucky bag extract contains 0.01 to 5% by weight relative to the total weight of the cosmetic composition It is characterized by. When the content of the extract is less than 0.001% by weight, no skin improvement effect is observed, and when the content of the extract is more than 30.0% by weight, the degree of improvement of the skin improvement effect against the increase of the content is insignificant, and there is a problem in the safety and stability of the formulation and economics. It is not an enemy.

The lucky bag extract extracts antioxidant effect (Experimental Example 1, Experimental Example 2), fibroblast proliferation effect (Experimental Example 3), MMP-1 production inhibitory effect (Experimental Example 4) and UV-induced MMP-1 production inhibitory effect (Experimental Example 5), Collagen Synthesis Enhancement Effect (Experimental Example 6), Melanin Production Inhibition Effect (Experimental Example 7), Cytotoxicity Relaxation Effect by Ultraviolet Irradiation (Experimental Example 8), Inflammatory Cytokine Expression Inhibition Effect by Ultraviolet Irradiation (Experimental example 9), skin elasticity improving effect (experimental example 10), skin wrinkle improvement effect (experimental example 11), and moisturizing effect (experimental example 12) are excellent.

Therefore, the cosmetic composition of the present invention comprising an extract of the bag with such a variety of functions is excellent antioxidant effect, skin aging effect, human fibroblast activity effect, skin whitening effect, skin damage relieving effect by ultraviolet rays, irritation relief effect It is characterized by having skin elasticity improving effect, wrinkle improvement effect and moisturizing effect.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above active ingredients as active ingredients, for example, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Phosphorus aids, and carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether. Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention is applied to the skin composition of the cosmetic composition containing the extract of the lucky bag is an active ingredient, the antioxidant effect, fibroblast proliferation effect, MMP-1 production inhibitory effect, MMP-1 production inhibitory effect by UV induction, Collagen synthesis enhancement effect, melanin production inhibitory effect (skin whitening effect), UV radiation remission cytotoxicity effect, UV irradiation inflammatory cytokine expression inhibition effect (skin damage inhibition effect), skin elasticity improvement effect, wrinkle improvement effect and It provides a makeup method characterized in that it has a moisturizing effect.

The cosmetic method of the present invention refers to all cosmetic methods using the cosmetic composition of the present invention. That is, all the methods known in the art using the cosmetic composition belong to the makeup method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.

If the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When carrying out the cosmetic method using the cosmetic composition containing the extract of the lucky bag of the present invention, excellent antioxidant effect, fibroblast proliferation effect, MMP-1 production inhibitory effect, MMP-1 production inhibitory effect by UV induction, collagen synthesis enhancement Anti-aging effects such as melanin production inhibitory effect, anti-cytotoxic effect by UV irradiation, inflammatory cytokine expression by UV irradiation, skin elasticity improvement and skin wrinkle improvement effect, skin whitening effect, skin damage prevention Effect, skin irritant effect and moisturizing effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One: With lucky bag  Extract manufacturer

The dried and dried bags of lucky bags were refluxed three times for 5 hours with 70% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 캜 or lower. The bag extract was prepared by dispersing / dissolving using a 50% butylene glycol solution such that the vacuum concentrate and the freeze-dried were 1% by weight.

Experimental Example  One: With lucky bag  Extract NBT Measure antioxidant effect

Experimental Example 1 is a comparison group of vitamin C and BHT (Butylated Hydroxytoluene), a substance known to have an antioxidant effect, in order to confirm the antioxidant effect of the extract of the lucky bag obtained in Preparation Example 1, the antioxidant effect by the NBT method (Active oxygen scavenging rate) was measured.

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by NBT method, and the test substance (Lucky bag extract, vitamin C, BHT) removes free radicals, that is, active oxygen Evaluate% erase. Free radicals are reacted with Nitro Blue Tetrazolium (NBT) to determine the percentage of active oxygen scavenging (%) by measuring the resulting blue at wavelength 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to the vial bottle and add 0.1ml of sample solution to it and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

④ The blank of the sample solution is measured in the same manner as in the case of measuring the absorbance St, except that distilled water is used instead of the above 6 to measure the absorbance So.

⑤ The absorbance test is performed in the same manner as in the case of measuring the absorbance St, except that distilled water is used instead of the sample solution.

⑥ The blank of the blank test was measured in the same manner as when the absorbance St was measured except that the sample solution and 6 were used instead of distilled water, and the absorbance Bo was measured.

Active oxygen scavenging rate (%) was calculated by Equation 1, the results are shown in Table 1.

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme in sample solution

Bo: absorbance at 560 nm before reaction in the absence of enzyme in blank test solution

Name of sample Treatment concentration (%, w / v) Antioxidative effect(%) Lucky bag extract
(Example 1) 0.01 99.2 vit-C 0.01 93.1 BHT 0.01 90.2

Experimental results were able to confirm the excellent antioxidant effect in all samples tested at 0.01% concentration as shown in Table 1. Among them, the bag extract of the present invention was confirmed to have a better antioxidant effect compared to vitamin C, BHT at the same concentration.

Experimental Example  2: With lucky bag  Extract DPPH Measure antioxidant effect

Experimental Example 2 is an antioxidant effect (free radical scavenging rate) using the DPPH method with a vitamin C, a substance known to have an antioxidant effect, in order to measure the antioxidant effect of the extract of the lucky bag obtained in Preparation Example 1 ) Was measured.

The DPPH method measures the antioxidant effect by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The free radical scavenging rate (%) is measured at a wavelength of 560 nm by comparing the degree of DPPH reduction by the test substance (Lack bag extract, vitamin C) to reduce the absorbance.

The reagent used was 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) as 61.88 mg in methanol to make 100 ml.

As a measuring method

① Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and incubate for 10 minutes at 25 ° C.

(2) Then measure the absorbance St at 560nm.

③ The blank of the sample solution is measured in the same manner as when the absorbance St is measured except that methanol is used instead of the 0.1 mM DPPH solution to measure the absorbance So.

④ The blank test is performed in the same manner as in the case of measuring the absorbance St, except that distilled water is used instead of the sample solution to measure the absorbance Bt.

⑤ Blank in the blank test was measured in the same manner as when the absorbance St was measured except that methanol was used instead of the 0.1 mM DPPH solution and distilled water was used instead of the sample solution.

The result of free radical scavenging rate (%) was calculated by Equation 2, and the results are shown in Table 2 below.

St: absorbance at 560 nm after free radical scavenging of sample solution

Bt: absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction without free radicals in sample solution

Bo: absorbance at 560 nm before reaction without free radicals in blank test solution

Name of sample Treatment concentration (%, w / v) Antioxidative effect(%) Lucky bag extract (Production example 1) 0.01 98.9 vit-C 0.01 92.5

As a result, as shown in Table 2, it is confirmed that the lucky bag extract has a better antioxidant effect than the antioxidant effect of vitamin C, a substance known to have an antioxidant effect at a concentration of 0.01%.

Experimental Example  3. With lucky bag  Fibroblast Proliferation Effect of Extracts

Fibroblasts (Korean Cell Line Bank, South Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, United States) and 37 ° C. After 24 hours of incubation in the serum-free DMEM medium to which the bag extract of Preparation Example 1 was added to the final concentration of 100 ㎍ / ㎖ and added in a serum-free DMEM medium containing no bags extract as a control for 48 hours Incubated with

 After incubation, MTT (Sigma, US) solution (3 mg / ml) was added to compare cell viability, and cell viability was measured at 570 nm using ELISA READER (Molecular Devices, USA). The fibroblast proliferation rate (%) was calculated according to the experimental results are shown in Table 3 below. TGF-β was used as a positive control.

Fibroblast Proliferation Effect of Extracts from Lucky Bags Test substance concentration (%) Fibroblast proliferation rate (%) TGF-β (10 ng / ml) 146.0 Lucky bag extract 172.5

As shown in the results of Table 3, the extract of the lucky bag of the present invention showed a growth rate of 172.5% when the fibroblast proliferation rate was measured at a concentration of 100 μg / ml. Even considering the difference between the concentration of TGF-β (10 ng / ml), which is known to have a fibroblast proliferation effect, and the concentration of the extract of the lucky bag (100 ㎍ / ml) of the present invention, the level of the extracts mixed with various substances The fibroblast proliferation rate as described above shows that the lucky bag extract of the present invention has a fibroblast proliferation effect.

Experimental Example  4. With lucky bag  Extract MMP -1 suppression effect

This Experimental Example 4 is to determine whether or not the lucky bag extract obtained in Preparation Example 1 has an effect of inhibiting the production of collagen degrading enzyme MMP-1, which has the effect of inhibiting the production of MMP-1 TGF-β (10 ng / ml, Roche, United States), a known substance, was used as a comparison group to determine the effect of inhibiting the production of MMP-1.

Fibroblasts (Korean Cell Line Bank, South Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, United States) and 37 ° C. After 24 hours of incubation in the serum-free DMEM medium to which the bag extract of Preparation Example 1 was added to the final concentration of 100 ㎍ / ㎖ and added in a serum-free DMEM medium containing no bags extract as a control for 48 hours Incubated with

After incubation, the supernatant of each well was collected and the amount of ng / ml of newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA), and MMP-1 production according to Equation 4 below. The percent inhibition was calculated and the results are shown in Table 4.

Test substance Inhibition rate of MMP-1 production (%) Lucky bag extract (production example 1) 72.5 TGF-β 70.1

As shown in the results of Table 4, the extract of the lucky bag of the present invention showed an inhibition rate of 72.5% when the inhibition rate of MMP-1 production was measured at a concentration of 100 µg / ml. In view of the difference between the concentration of TGF-β (10 ng / ml), which is known to have an inhibitory effect on the production of MMP-1, and the concentration of the extract of the lucky bag (100 ㎍ / ml) of the present invention, The above-described degree of inhibition of MMP-1 at the level of extract shows that the extract of the lucky bag of the present invention has an inhibitory effect on MMP-1 production.

Experimental Example  5. With lucky bag  By UV induction of extract MMP -1 suppression effect

In general, MMP-1 is known to increase its amount by ultraviolet light. Therefore, the experiment was performed to determine whether the bags of the present invention inhibit the amount of MMP-1 that is increased by the ultraviolet light production. MMP-1 production inhibition effect measurement by UV induction was carried out in the same manner as in Experiment 4 except that a certain amount of UVB treatment prior to sample treatment. Lucky bag extract used in this experiment was obtained in Preparation Example 1, but is not limited thereto.

MMP-1 production inhibition rate (%) of the extract of the lucky bag was calculated according to Equation 4, the results are shown in Table 5 below. At this time, TGF-β (10ng / ml, Roche, United States of America), a substance known to have an inhibitory effect on MMP-1 production, was used as a comparison group, and the extract of lucky bag was carried out at a concentration of 0.01% (w / v). It was.

Test substance Inhibition rate of MMP-1 production (%) Lucky bag extract (production example 1) 75.9 TGF-β 65.2

Table 5 shows the results of measuring the amount of collagen degrading enzyme MMP-1 secreted in the culture medium to which the cultured fibroblasts were added UVB irradiation and TGF-β culture medium after UVB irradiation. Lucky bag extract of the present invention exhibited an inhibition rate of 75.9% when the MMP-1 production inhibition rate was measured at a concentration of 100 µg / ml. Even considering the difference between the concentration of TGF-β (10 ng / ml), which is known to have an inhibitory effect on MMP-1 production, and the concentration (0.01% (w / v)) of the extract of the lucky bag of the present invention, The expression of MMP-1 inhibition at the level of the mixed extract shows that the lucky bag extract of the present invention has an inhibitory effect on MMP-1 production.

Experimental Example  6. With lucky bag  Enhancement of Collagen Synthesis by Extracts

Fibroblasts, human normal epithelial cells, were seeded in 48-well microplates at 1 × 10 ⁶ cells per well and incubated in DMEM medium for 24 hours. The bags were further incubated for 48 hours in serum-free DMEM medium added to the final concentration of 100 ㎍ / ㎖ and serum-free DMEM medium containing no bags bag extract as a control. After incubation, the supernatant of each well was collected and measured in ng / ml by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a collagen kit (Takara, Japan). Measured.

In the comparison group, TGF-β (10 ng / ml, Roche, United States), a substance known to have an effect of enhancing collagen synthesis, was used as a comparison group, and the extract of lucky bag was 0.01% (w / v). The experiment was conducted. Collagen biosynthesis increase rate (%) was calculated according to the following equation (5), the results are shown in Table 6.

Test substance Increase in collagen biosynthesis (%) Lucky bag extract (production example 1) 90.2 TGF-β 89.6

Table 6 shows the results of measuring the collagen biosynthesis enhancement effect of the culture medium containing the lucky bag extract and TGF-β using fibroblasts. According to Table 6, it can be seen that the lucky bag extract of the present invention shows a biosynthesis rate of 90.2% when the collagen biosynthesis growth rate is measured at a concentration of 0.01%. Even considering the difference between the concentration of TGF-β (10 ng / ml) and the concentration of the extract of the present invention (0.01% (w / v)), a substance known to enhance collagen synthesis, several kinds of substances are mixed. As shown in the collagen biosynthesis rate as described above at the extracted extract level, the lucky bag extract of the present invention shows that the collagen synthesis enhancing effect.

Experimental Example  7: With lucky bag  Inhibitory Effects of Extracts on Melanin Production on B16F10 Melanogenic Cells

Experimental Example 7 is to determine the whitening effect by looking at the degree of inhibition of melanin production for B16F10 melanin forming cells in order to confirm the whitening effect of the bag extract obtained in Preparation Example 1. B16F10 melanin forming cells used in Experimental Example 7 are cell strains derived from mice, cells that secrete a black pigment called melanin, and the degree of reduction of melanin black pigment is reduced by treating a sample during artificial culture of these cells. It was. The B16F10 melanin forming cells used in the present experimental example were used from the American Type Culture Collection (ATCC).

The melanin biosynthesis inhibitory effect of B16F10 melanin forming cells was measured as follows.

B16F10 melanin-forming cells were divided into 6-well plates at a concentration of 2 X 10 ⁶ per well, and the cells were attached and incubated for 72 hours by treating the extract of the bags according to Preparation Example 1 at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, and the cells were counted and centrifuged to recover the cells. Quantification of intracellular melanin was performed with a slight modification of the method of Rotan: Cancer Res., 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin and the absorbance of melanin was measured at 405 nm with a microplate reader. The inhibition rate (%) of melanin formation was measured. The melanin production inhibition rate (%) of B16F1 melanin forming cells was calculated by the following Equation 6, the results are shown in Table 7. At this time, hydroquinone and arbutin, which are known to have melanin production inhibitory effects, were used as a comparison group.

A: Melanin amount in wells without sample

B: Amount of melanin in the well to which the sample was added

Name of sample Treatment concentration
(%, w / v) Melanin production inhibition rate (%) Lucky bag extract
(Manufacture example 1) 0.1 0.05 Hydroquinone 0.1 0.08 Arbutin 0.1 0.03

As can be seen from the results of Table 7, the lucky bag extract of the present invention can be seen that significantly inhibit the production of melanin in B16F10 melanin forming cells. Lucky bag extract has a melanin production inhibition value of 0.05% at 0.1% concentration, melanin is relatively lower than hydroquinone when compared to arbutin and hydroquinone, substances known to have a whitening effect at the same concentration Although it showed a production inhibitory effect, it showed an excellent melanin production inhibitory effect than arbutin. Therefore, it can be seen that the cosmetic composition comprising the extract of the lucky bag of the present invention has an excellent effect on inhibiting melanin production.

Experimental Example  8: With lucky bag  Cytotoxicity-Reducing Effect of Extracts by UV Irradiation

Experimental Example 8 was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the extract of the lucky bag obtained in Preparation Example 1.

Fibroblasts (fibroblasts) were placed in a 24-well test plate 5 x 10 ⁴ pieces and attached for 24 hours. Each well was washed once with PBS and 1000 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10 mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B15W, Sankyo Dennki, Japan), after which PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. The bags were treated with the extract and incubated for 24 hours. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed and 500 μl of isopropanol-HCl (0.04N) was added thereto. The cells were lysed by shaking for 5 minutes and 100 μl of supernatant was transferred to a 96-well test plate, followed by measurement of 565 nm absorbance in a microplate reader. TGF-β was used as a positive control.

Cell viability (%) was measured by Equation 7 below, and cytotoxicity relaxation rate (%) by UV irradiation was calculated by Equation 8.

Bo: Absorbance at 565 nm of the well of the cell culture medium alone

Bt: Absorbance at 565 nm of a well that underwent chromogenic reaction in a sample not treated with the sample

St: 565 nm absorbance of the wells that reacted the sample treated wells

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: Cell viability of wells irradiated with ultraviolet light and not treated with the sample

St: Cell survival rate of wells treated with UV

Name of sample Treatment concentration (%, w / v) Cytotoxic Relaxation Rate (%) Lucky bag extract 0.0125 52 0.025 74 0.050 93 TGF-β 10 ng / ml 85

According to Table 8, the cytotoxicity relaxation rate by UV at the concentration of 0.0125% of the extract of the lucky bag was 52%, 74% at 0.025% concentration, 93% at 0.050% concentration. The lucky bag was shown to increase the cytotoxicity reduction rate in a concentration-dependent manner as the treatment concentration of the extract increases, it can be seen that it effectively protects the cytotoxicity caused by ultraviolet rays. In addition, TGF-β, a substance known to have a cytotoxic effect, exhibits 85% cytotoxicity reduction at a concentration of 10 ng / ml, and thus, the lucky bag extract of the present invention effectively prevents cell damage caused by UV irradiation even at low concentrations. I could know the defense.

Experimental Example  9: With lucky bag  Inhibitory Effects of Extracts on Inflammatory Cytokine Expression by Ultraviolet Irradiation

Experimental Example 9 is to evaluate the inhibitory effect of the inflammatory cytokine expression expressed by ultraviolet irradiation of the extract of the lucky bag obtained in Preparation Example 1, 24-bit fibroblasts isolated from human epidermal tissue 5 x 10 ⁴ pieces were placed in a well test plate and attached for 24 hours.

Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with 10 mJ / cm 2 of UV light using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), after which PBS was removed and FBS was not added to the cell culture medium (DMEM). Medium) 350 μl was added.

It was incubated for 5 hours after the treatment of the extract of the lucky bag to be evaluated here. 150 µl of the culture supernatant was taken to quantify IL-1α, and thus, the effect of inhibiting the inflammatory cytokine expression of the bag extract was determined. The amount of IL-1α was quantified using an enzyme-linked immunosorbent assay, and ketoprofen, known as an IL-1α inhibitor, was used as a positive control. The percent inhibition of expression of inflammatory cytokines (IL-1α) was calculated by the following equation (9).

Bo: IL-1? Production in wells without UV irradiation

Bt: IL-1? Production in wells irradiated with ultraviolet light and not treated with the sample

St: IL-1α production amount of wells that were irradiated with UV light

Name of sample Treatment concentration (%, w / v) Inflammatory cytokine
% Inhibition of expression Lucky bag extract 0.0125% 55 0.025% 62 0.050% 81 Ketoprofen 100 μg / mL 48

According to the above Table 9, the bag extract of the present invention inhibits the production of the inflammatory cytokine IL-1α by the ultraviolet ray at 55% at 0.0125% concentration, and inhibits the production of IL-1α when treated at a concentration of 0.025% When it was 62% inhibited and 0.050% concentration was processed, it turned out that 81% of IL-1 (alpha) production was suppressed. Given the difference in concentration of ketoprofen (100 μg / mL), a substance known to have an inhibitory effect on the production of inflammatory cytokines, IL-1α, 48% inhibition showed that the lucky bag extract of the present invention was induced by ultraviolet rays. It can be seen that it effectively protects the occurrence of inflammation even at low concentrations.

Hereinafter, on the basis of the results of the above experimental examples, to present a cosmetic composition containing the extract as an active ingredient with the lucky bag, but the formulation of the present invention is not necessarily limited thereto.

Formulation Example  1. Flexible Cosmetics

Formulation examples of the flexible longevity was prepared as shown in Table 10 according to a conventional method.

ingredient Content (unit: weight%) Lucky bag extract
glycerin
1.3-butylene glycol
PEG 1500
Allantoin
DL-panthenol
E.T.A.-2NA
Benzophenone-9
Sodium hyaluronate
ethanol
Octicdodeceth-16
Polysorbate 20
Preservative, fragrance, coloring
Purified water 3.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.1
a very small amount
Balance Sum 100

Formulation Example  2. Converging Cosmetics

Formulation examples of convergent longevity was prepared as shown in Table 11 in accordance with a conventional method.

ingredient Content (unit: weight%) Lucky bag extract
glycerin
1.3-butylene glycol
Allantoin
DL-panthenol
E.T.A.-2NA
Benzophenone-9
Sodium hyaluronate
ethanol
Polysorbate 20
Witch hazel extract
Citric acid
Preservative, fragrance, coloring
Purified water 1.5
2.0
2.0
0.2
0.2
0.02
0.04
3.0
15.0
0.3
2.0
a very small amount
a very small amount
Balance Sum 100

Formulation Example  3. Nutritional Cosmetics

Formulation examples of nutrient lotion was prepared as shown in Table 12 in a conventional manner.

ingredient Content (unit: weight%) Lucky bag extract
Stearyl alcohol
lanolin
Polysorbate 60
Sorbitan stearate
Cured Vegetable Oil
Mineral oil
Squalane
Trioctanoine
Dimethicone
Tocopherol Acetate
Carboxy Vinyl Polymer
glycerin
1.3-butylene glycol
Sodium hyaluronate
Tri ethanolamine
Preservative, fragrance, coloring
Purified water 1.5
1.5
1.5
1.3
0.5
1.0
5.0
3.0
2.0
0.8
0.5
0.12
5.0
3.0
5.0
0.12
a very small amount
Balance Sum 100

Formulation Example  4. Nutrition Cream

Formulation of nutrition cream was prepared according to the conventional method as shown in Table 13.

ingredient Formulation Example 4 Comparative Formulation Example 1 Comparative Formulation Example 2 Content (unit: weight%) Lucky bag extract
Adenosine
Lipophilic monostearate
Cetearyl Alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Cured Vegetable Oil
Squalane
Mineral oil
Trioctanoine
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaluronate
Preservative, fragrance, coloring
Purified water 3.0
-
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance -
-
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance -
3.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100 100 100

Formulation Example  5. Massage Cream

Formulation example of the massage cream was prepared according to the conventional method as shown in Table 14.

ingredient Content (unit: weight%) Lucky bag extract
Chin type glycerin monosuccinic acid
Stearyl alcohol
Stearic acid
Polysorbate 60
Sorbitan stearate
Isostearyl Isosterate
Squalane
Mineral oil
Dimethicone
Hydroxyethyl Cellulose
glycerin
Triethanolamine
Preservative, fragrance, coloring
Purified water 3.0
1.5
1.5
1.0
1.5
0.6
5.0
5.0
5.0
0.5
0.12
6.0
0.7
a very small amount
Balance Sum 100

Formulation Example  6. Essence

Formulation examples of the essence was prepared according to the conventional method as shown in Table 15 below.

ingredient Content (unit: weight%) Lucky bag extract
glycerin
Betaine
Fiji 1500
Allantoin
DL-panthenol
E.T.A.-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaluronate
Carboxy Vinyl Polymer
Triethanolamine
Octyldodecanol
Octyldodeceth -16
ethanol
Preservative, fragrance, coloring
Purified water 5.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
Balance Sum 100

Formulation Example  7. Pack

Formulation examples of the pack was prepared as shown in Table 16 in the usual manner.

ingredient Content (unit: weight%) Lucky bag extract
Polyvinyl alcohol
Cellulose gum
glycerin
Fiji 1500
Cyclodextrin
DL-Panthenol
Allantoin
Monomethylammonium Glycyric Acid
Nicotinamide
ethanol
Fiji 40 Cured Castor Oil
Incense, pigment, preservative
Purified water 3.0
15.0
0.15
3.0
2.0
0.15
0.4
0.1
0.3
0.5
6.0
0.3
a very small amount
Balance Sum 100

Experimental Example  10. With lucky bag  Effect of Extract on Skin Elasticity

Clinical experiment was conducted on the skin elasticity improving effect of the cosmetic bag containing the extract of the present invention. Clinical trials were evaluated through the actual use test of each cosmetic.

The experiment was carried out by comparing the nutrient cream of Formulation Example 4, which contains the extract of lucky bag and extract, and the cream of which the lucky bag extract was replaced with purified water as Comparative Formulation Example 1, adenosine which is known to have an anti-wrinkle effect. In Comparative Formulation Example 2, the effect of improving skin elasticity due to the use of cosmetics in humans was confirmed and compared.

Twenty experimenters (20-35 year old females) were divided into two groups, one group for Formulation 4 on the right side of the face, Comparative Formulation Example 1 for the left side of the face, and the other. Example 2 was applied twice a day for 8 consecutive weeks.

The skin elasticity improvement effect after the completion of the experiment was measured by using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months, respectively, and the experimental results are shown in Table 17 below. It is represented by the ΔR8 value of SEM 575. This? R8 value indicates the nature of the viscoelasticity of the skin.

Experimental product Skin elasticity effect (△ R8) Formulation Example 4 (Lucky bag extract) 0.22 Comparative Formulation Example 1 (purified water) 0.09 Comparative Formulation Example 2 (Adenosine) 0.20

As shown in Table 17, the cream of Formulation Example 4 containing the extract of the lucky bag as an active ingredient is about 2.4 times more than the cream of Comparative Formulation Example 1 does not contain the lucky bag extract extracts It was shown that the cosmetic composition containing the lucky bag with the extract of the present invention has an excellent skin elasticity improvement effect compared to the cosmetic composition of Comparative Formulation Example 2 containing adenosine.

Experimental Example  11. With lucky bag  Skin Wrinkle Improvement Effect of Extracts

Wrinkle improvement effect of the cosmetic bag containing the extract of the present invention was evaluated through a practical test. The test group was divided into three groups, each of 20 groups of 60 females in the Comparative Formulation Example 1 treatment group, Comparative Formulation Example 2 treatment group, and Formulation Example 4 experiment group. The degree of wrinkle improvement was confirmed by the evaluation. In this case, the cream that replaced the lucky bag extract shown in Table 13 with purified water as Comparative Formulation Example 1, 3% (v / v) of the lucky bag extract with 3% (v / v) of adenosine In Comparative Formulation Example 2, a cream containing 3% (v / v) of lucky bag extract was used as Formulation Example 4. The results of the wrinkle improvement effect based on this evaluation are shown in Table 18 below.

Wrinkle improvement effect of nutrition cream containing extract of lucky bag (visual evaluation) Wrinkle improvement Effective rate (%) Great slightly none Formulation Example 4 (Lucky bag extract) 18 One One 52 Comparative Formulation Example 1 (purified water) 0 One 19 3 Comparative Formulation Example 2 (Adenosine) 15 4 One 45

As can be seen from the results of Table 18, the nutritional cream of Formulation Example 4 containing the bag extract of the present invention showed a higher wrinkle improvement effect than the cream of Comparative Formulation Example 1 does not contain the bag extract It was found that the wrinkle improvement effect was superior to that of the cream of Comparative Formulation Example 2 containing adenosine. In addition, skin irritation could not be observed in most of the subjects who applied the cosmetic containing the extract of the lucky bag of the present invention to the skin.

Experimental Example  12: With lucky bag  Containing extract Cosmetic  skin Moisturizing  Improvement effect

12-1. Device evaluation

In order to determine the effect of improving the skin moisturizing effect of the cosmetic bag containing the extract of the present invention was carried out as follows. Divided into two sets of twenty people per trillion for 25 people in their 20s to 40s without skin diseases, the flexible cosmetic composition containing the extract of the lucky bag of the present invention for each group by the composition shown in Table 19 Formulation Example 8 At this time, for comparison, a control bag (Comparative Formulation Example 3) containing no bags extract was prepared together, and was applied to the face and forearm twice a day for 1 month.

ingredient Formulation Example 8
(Lucky bag extract) Comparative Formulation Example 3
(Purified water) Lucky bag extract 3.0 - glycerin 5.1 5.1 Propylene glycol 4.2 4.2 Tocopheryl acetate 3.0 3.0 Liquid paraffin 4.6 4.6 Triethanolamine 1.0 1.0 Squalane 3.1 3.1 Macadamia Nut Oil 2.5 2.5 Polysorbate 60 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 Propylparaben 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

Measure the skin conductivity using the corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ° C, 40% humidity) before starting the application. Skin conductivity was measured to assess the rate of increase. The results are shown in Table 20 below.

Skin conductivity increase rate (%) sample 1 week later after 2 weeks 4 weeks later Formulation Example 8
(Lucky bag extract) 57 63 58 Comparative Formulation Example 3
(Purified water) 11 13 12

As shown in the results of Table 20, in the case of Formulation Example 8, which is a cosmetic containing the extract of the lucky bag of the present invention, the skin conductivity increase rate was very excellent compared to Comparative Formulation Example 3. In general, since the skin conductivity is proportional to the skin moisture content, it can be seen from the above results that the cosmetic composition containing the extract of the lucky bag in the present invention maintains the skin moisture content higher than that of the cosmetic water containing purified water. Therefore, the cosmetic composition containing the extract of the lucky bag of the present invention is excellent in improving the skin moisturizing power.

12-2. Clinical evaluation

Clinical moisturizing effect on the cosmetic composition prepared according to the above prescription example was measured as follows. The questionnaire selected 20 healthy men and women who feel dry skin, divided the upper arm into 10 sites, and applied each sample twice a day for 4 weeks. The moisturizing effect was evaluated using Corneometer CM 820 (Corage + Kazaka, Germany) every two weeks from the start of the practical test and after the end. The experimental results are shown in Table 21 below.

Improved skin moisturizing effect of lucky bag extract division Before use 2 weeks after use 4 weeks after use Formulation Example 8 (Lucky bag extract) 32.5 48.6 58.8 Comparative Formulation Example 3 (purified water) 32.6 33.6 34.6

As shown in Table 21, after two weeks of use and four weeks after use, the result of measuring the effect of improving the moisturizing power, using the cosmetic bag containing the extract of the lucky bag of the present invention, the effect of improving the moisturizing power than using purified water instead of the extract Was found to be very excellent.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (10)

Lucky bag is an antioxidant cosmetic composition containing the extract as an active ingredient. Cosmetic bag for preventing skin aging containing the extract as an active ingredient. Cosmetic bag for moisturizing the skin containing the extract as an active ingredient. Cosmetic bag for improving skin wrinkles containing an extract as an active ingredient. Cosmetic bag for skin whitening containing an extract as an active ingredient. A cosmetic composition for inhibiting skin damage containing an extract as an active ingredient. Cosmetic bag for skin irritation relief containing an extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 7, wherein the extract of the lucky bag is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to any one of claims 1 to 7, wherein the lucky bag extract is (a) water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, Solvent extraction using an extraction solvent selected from the group consisting of chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof, (b) supercritical extraction or (c) ultrasonic extraction. Cosmetic composition. delete