KR20070021853A - Composition for skin external application for skin moisturing effect, for reinforcement of skin barrier function, or for induction of skin keratinocytes differentiation comprising kaempferol - Google Patents

Abstract

The present invention relates to an external composition for skin moisturizing, skin barrier function enhancement and skin keratinocyte differentiation induction comprising camphorol as an active ingredient, wherein the camphorol is an acid, base, enzyme, or from a camphorol glycoside. It is obtained by separating camphorol through a decomposition reaction using the microorganism producing the enzyme, wherein the camphorol glycosides are derived from plants, especially green tea, characterized in that the camphorol content is 90% or more.

The camphorol according to the present invention exhibits the effect of promoting the differentiation of keratinocytes of the skin, restoring damaged skin barrier function and increasing skin moisturizing power. Therefore, the external composition for skin containing camphorol is used for skin moisturizing, skin barrier function, and skin keratinocyte differentiation, skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation. It prevents or improves, and may be usefully used as a cosmetic composition or pharmaceutical composition for skin moisturizing, skin barrier function enhancement and skin keratinocyte differentiation induction.

Camphorol, skin, moisturizing, external preparations, glycosides, acids, bases, enzymes, microorganisms, plants, green tea, barrier function, keratinocytes, atopy, cosmetics, pharmaceuticals, compositions.

Description

Composition for skin external application for skin moisturizing, enhancing skin barrier function, and inducing differentiation of keratinocytes containing camphorol as an active ingredient of skin keratinocytes differentiation comprising kaempferol}

1 is a graph showing the effect of camphorol according to the present invention on the recovery of damaged skin barrier.

2 is a graph showing the effect of the camphorol according to the present invention on the skin moisture content.

The present invention relates to an external composition for skin moisturizing, enhancing skin barrier function, and inducing differentiation of keratinocytes, wherein the camphorol promotes the differentiation of keratinocytes and damaged skin barrier. As it has the effect of increasing the recovery and skin moisturizing ability of the skin, the skin moisturizing effect of preventing or improving skin dryness, atopic symptoms, and contact dermatitis caused by incomplete epidermal differentiation by containing the camphorol, The present invention relates to a skin external preparation composition for enhancing skin barrier function and inducing differentiation of keratinocytes from skin.

Skin plays a very important role as a barrier to protect individuals from the outside. The barrier function is to protect against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and to prevent excessive release of moisture through the skin, and this protection function is a stratum corneum composed of keratinocytes. The function can be maintained only when is normally formed.

The outermost stratum corneum (horney layer) is formed from keratinocytes and consists of the differentiated keratinocytes and the surrounding lipid layer (Marcelo CL et al, J. Invest. Dermatol. , 80 , pp37-44, 1983)

Keratinocytes are characteristic cells whose basal cells, which continuously proliferate in the epidermal layer, undergo morphological and functional changes in stages and rise to the surface of the skin. Exfoliated and new keratinocytes take over their function. This repetitive sequence of changes is called "epidermal differentiation" or "keratinization."

In addition, during keratinization, keratinocytes form a natural moisturizing factor (NMF) and intercellular fats (ceramides, cholesterol, fatty acids), forming the stratum corneum, making the stratum corneum firm and flexible, thus creating a skin barrier ( It has a function as a skin barrier.

The stratum corneum can easily lose its function due to excessive face washing, lifestyle factors such as bathing, environmental factors such as dry air pollutants, and endogenous diseases such as atopic or senile skin. Recently, due to a variety of factors, more and more skin complaints and dry skin complaints are increasing in recent years.

Therefore, various studies have been conducted to supply moisture from the outside or to prevent water loss from the body in order to maintain proper skin moisture, and various moisturizers having water retention capabilities have been developed. As skin moisturizers, it is common to use lipid components such as ceramides and substances that can increase the water retention in the stratum corneum such as essential fatty acids and lipid complexes (Rawlings AV et al, J. Invest. Dermatol. , 5, pp731-). 741, 1994)

However, in recent years, the risk factors for the skin are gradually increasing, and the changes in dietary patterns slow down the formation and dropping rate of the stratum corneum, and the amount of moisturizing factors and lipids in the stratum corneum decreases due to the deterioration of the function of keratinocytes. There is a growing number of people with skin that do not function as a skin barrier. This disruption of skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis, and psoriasis. These diseases can be expected to alleviate symptoms only with a conventional moisturizer having a water retention function, but fundamental healing is difficult.

In order to solve the above problems, the inventors of the present invention are trying to find a substance exhibiting excellent skin moisturizing effect among natural effective substances, and an acid and a base from camphorol, especially among camphorol glycosides isolated from plants, especially green tea. The present invention was completed by confirming that camphorol obtained through a decomposition reaction using an enzyme or a microorganism producing the enzyme has the above effect.

Accordingly, the technical problem to be achieved by the present invention is the differentiation of keratinocytes of camphorol obtained through a decomposition reaction using an acid, a base, an enzyme or a microorganism producing the enzyme from a camphorol, especially a camphorol glycoside derived from green tea. The purpose of the present invention is to investigate the promoting effect, the restoration of damaged skin barrier function, and the increase of skin moisturizing effect, and to provide the external composition for skin moisturizing, strengthening skin barrier function, and inducing differentiation of keratinocytes. do.

In order to achieve the above object, the present invention provides a skin external preparation composition for moisturizing skin containing camphorol as an active ingredient.

The present invention also provides a skin external preparation composition for enhancing skin barrier function containing camphorol as an active ingredient.

The present invention also provides a topical skin composition for inducing skin keratinocyte differentiation containing camphorol as an active ingredient.

In the external preparation composition for skin, the camphorol is characterized in that the camphorol content is 90% or more.

In the external preparation composition for skin, the camphorol may be obtained by separating camphorol from a camphorol glycoside through a decomposition reaction using an acid, a base, an enzyme, or a microorganism producing the enzyme.

The camphorol glycosides are derived from plants, preferably from green tea.

In the decomposition method, the acid is characterized in that at least one acid selected from the group consisting of hydrochloric acid, sulfuric acid and nitric acid, or a mixed solvent of these acids and at least one alcohol selected from the group consisting of ethanol, methanol and butanol.

In the decomposition method, the base is at least one base selected from the group consisting of sodium hydroxide and potassium hydroxide or a mixed solvent of these bases with at least one alcohol selected from the group consisting of ethanol, methanol and butanol. .

In the decomposition method, the enzyme is characterized in that the exo (glucose) glycolytic enzyme to separate the camphorol by removing the sugar portion from the camphorol glycosides.

In the degradation method, the exo-glucose degrading enzyme is a glucosidase, arabinosidase, rhamnosidase, xylosidase, cellulase, hess Consists of hesperidinase, naringinase, glucuronidase, pectinase, galactosidase and amyloglucosidase It is characterized in that at least one selected from the group.

In the decomposition method, the enzyme-producing microorganisms include the genus Aspergillus, Bacillus, Bacillus, Penicillium, Rhizopus, Rhizomucor, At least one selected from the group consisting of genus talomyces, genus bifidobacterium, genus mortierella, genus cryptoococcus, and genus microbacterium. It is done.

The external preparation composition for skin is characterized in that it prevents or improves skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation.

The external composition for skin is characterized in that the cosmetic composition.

The external preparation composition for skin is characterized in that the pharmaceutical composition.

The external preparation composition for skin is characterized in that it contains camphorol in an amount of 0.0001 to 10% by weight based on the total weight of the composition.

Hereinafter, the present invention will be described in detail.

The camphorol of the present invention is preferably obtained through a decomposition reaction using an acid, a base, an enzyme, or a microorganism producing the enzyme of a green tea extract containing a camphorol glycoside obtained from plants, in particular, green tea.

First, from about 1 to 6 times, preferably about 3 times, water to green tea leaves or green tea seeds to prepare green tea extracts, which are plant extracts containing camphorol glycosides, using water or organic solvents from plants, especially green tea. One or more organic solvents selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, and chloroform, or a mixed solvent of these organic solvents with water are extracted, degreased by stirring 1-5 times at room temperature, and then degreased. About 1 to 8 times, preferably about 4 times of water or the organic solvent is added to the dried plants, and the mixture is extracted under reflux 1 to 5 times, and then deposited at 10 to 20 ° C. for 1 to 3 days, followed by filtration and centrifugation. The residue and the filtrate were separated through the mixture, and the extract obtained by concentrating the separated filtrate under reduced pressure was suspended in water, and then the dye was removed using ether, etc. After extraction 1 to 5 times using an organic solvent, the obtained organic solvent layer was concentrated under reduced pressure to obtain the organic solvent extract, dissolved in a small amount of methanol, etc., and then a large amount of ethyl acetate was added to dry the resulting precipitate. In this way, the green tea extract containing the camphorol glycoside of the present invention can be obtained.

Then, the green tea extract containing the camphorol glycoside is hydrolyzed using an acid, a base, an enzyme, or a microorganism producing the enzyme to prepare camphorol derived from green tea.

At this time, when using an acid, to the green tea extract is added 0.1 to 2N concentration, preferably 1N concentration of acid or a mixed solvent of acid and alcohol (preferably 50% ethanol mixed solvent), 50 to 100 ℃, Preferably, the reaction solution may be obtained by hydrolysis by heating to reflux for 0.5 to 2 hours in an 80 ° C. water bath.

The acid may be one or more acids selected from the group consisting of hydrochloric acid, sulfuric acid and nitric acid, or a mixed solvent of these acids with one or more alcohols selected from the group consisting of ethanol, methanol and butanol.

In this case, when the base is used, the green tea extract is dissolved, and then a base or a mixed solvent (preferably 50% butanol mixed solvent) of 0.1N to 2N concentration, preferably 1N concentration, is added to 50 to 100 ° C. For example, the reaction solution may be hydrolyzed by heating to reflux for 1 to 12 hours in a 100 ° C. water bath.

The base may be one or more bases selected from the group consisting of sodium hydroxide and potassium hydroxide, or a mixed solvent of these bases with one or more alcohols selected from the group consisting of ethanol, methanol, butanol.

At this time, when using the enzyme, the green tea extract is dissolved in an acid buffer solution of 5 to 20 times, preferably about 10 times, then the enzyme is added and stirred for about 1 to 60 hours in a water bath of about 37 ℃, thin layer chromatography When the substrate is completely disappeared by the graph, the substrate is completely disappeared, and then heated in hot water (80 to 100 ° C.) for 5 to 15 minutes to terminate the hydrolysis reaction to obtain a reaction solution.

When utilizing the microorganism to produce the enzyme, the green tea extract is dissolved in 5 to 10 times, preferably about 10 times in ionized water, sterilized at 121 ℃ for 30 minutes, cooled to 30 ℃ and then pre-cultured microorganism liquid After inoculating with 5 to 10% of the amount and incubated at 30 ° C. for 2 to 5 days, the precipitate recovered by centrifugation at 5,000 to 10,000 rpm may be washed three times with distilled water and then centrifuged to obtain a reaction solution as a precipitate.

The enzyme or microorganism producing the enzyme may be an exo-glycolytic enzyme or a microorganism producing the exo-glycolytic enzyme, and the enzyme may separate the camphorol by removing the sugar portion from the camperol glycoside. Can be.

After hydrolysis using an acid, a base, an enzyme, or a microorganism producing the enzyme as described above, the reaction solution is concentrated under reduced pressure to remove the solvent, and alcohol is added to the residue, followed by stirring 1 to 5 times. The obtained filtrate was removed by filtration, and the filtrate was concentrated under reduced pressure to give a crude product. The crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to obtain a predetermined amount of camphorol. (High purity camphorol having a camphorol content of 90% or more) can be obtained.

In order to examine the moisturizing effect of camphorol obtained by the above method, the effect of promoting the differentiation of keratinocytes of camphorol obtained through the acid and base hydrolysis, enzymatic digestion, and microbial decomposition method, damaged skin barrier The effect of restoring the function and increasing the skin moisturizing amount was measured.

The camphorol according to the present invention can be used in a topical skin composition, which is not particularly limited in its formulation. For example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, body It may be formulated into cosmetic compositions such as essences, makeup bases, foundations, hair dyes, shampoos, rinses or body cleansers or pharmaceutical compositions such as ointments, gels, creams, patches or sprays.

Each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by the inventors.

The external preparation composition for skin of the present invention is characterized by containing the camphorol in an amount of 0.0001 to 10% by weight, respectively, based on the total weight of the composition. When the content is less than 0.0001% by weight, the skin moisturizing effect, skin barrier function, and skin keratinocyte differentiation inducing effect by the above components cannot be obtained, and when the content is 10% by weight or more, This is because the increase in effect is not large.

The formulation of the external preparation composition for skin of the present invention is as follows.

The external preparation composition for skin of the present invention is a cosmetic composition, characterized in that the amount of camphorol is contained in an amount of 0.0001 to 10% by weight based on the total weight of the total cosmetic composition.

In addition, the cosmetic composition according to the present invention, in addition to the above-mentioned camphorol, preferably containing other ingredients and the like that can give a synergistic effect to the main effect within the range that does not impair the main effect of the present invention. It's okay.

Although camphorol according to the present invention is preferably prepared in each cosmetic formulation and applied as it is, it can also be used as a general external formulation.

The cosmetic composition of the present invention can be used in a variety of cosmetics for skin moisturizing effect, skin barrier function strengthening effect, and skin keratinocyte differentiation induction effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, and essences.

Cosmetics to which the composition containing the camphorol of the present invention is added may take the form of a solution, an emulsion, a viscous mixture, or the like.

That is, the cosmetic of the present invention is not particularly limited in the formulation, for example, latex, cream, lotion, essence, pack, gel, powder, makeup base, foundation, lotion, ointment, patch, beauty liquid, cleansing foam, cleansing Formulations such as creams, cleansing water, body lotions, body creams, body oils, body essences, shampoos, rinses, body cleansers, soaps, hair dyes, sprays and the like.

In the cosmetic composition of each formulation, other ingredients in addition to the above camphorol may be appropriately selected by those skilled in the art without difficulty according to the formulation or use purpose of the other cosmetics.

The above-mentioned preparations may contain a skin absorption promoting substance to increase skin moisturizing effect, skin barrier function enhancing effect, and skin keratinocyte differentiation inducing effect.

In addition, the cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments and flavorings. , Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, any of the above components can be mix | blended within the range which does not impair the objective and effect of this invention.

The topical skin composition of the present invention is a pharmaceutical composition, wherein the amount of camphorol is 0.0001 to 10% by weight of the total pharmaceutical composition for skin moisturizing, enhancing skin barrier function, and inducing skin keratinocyte differentiation. do.

The pharmaceutical composition comprising the camphorol of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

As the dosage form, the pharmaceutical composition comprising camphorol according to the present invention may be used in any form suitable for pharmaceutical preparations, including external preparations such as ointments, gels, creams, patches, or sprays according to conventional methods. have.

The dosage of the preparation is different depending on the subject's age, sex, weight, symptoms, and method of administration, but it is preferable to apply it at 1.0 to 3.0 ml per day and apply it 1 to 5 times a day for more than one month.

On the other hand, the camphorol of the present invention has little toxicity and side effects, so can be used with confidence even for long-term use for the purpose of prevention.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the present invention is not limited only to these examples.

Example 1 Preparation of Green Tea Seed Extract

6 kg of hexane was added to 2 kg of green tea seeds, stirred and extracted three times at room temperature, and then degreased. 4 L of 80% methanol was added to 1 kg of degreased green tea seeds, and refluxed three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the extract obtained by concentrating the separated filtrate under reduced pressure was suspended in water, extracted five times with 1 L of ether to remove the pigment, and the aqueous layer was 1-butanol. Extracted three times with 500 mL. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain 1-butanol extract, which was dissolved in a small amount of methanol, and then added to a large amount of ethyl acetate, and the resulting precipitate was dried to give 250 g of green tea seed extract. .

Example 2 Preparation of Green Tea Leaf Extract

6 kg of hexane was added to 2 kg of green tea leaves, stirred and extracted three times at room temperature, and then degreased. 4 L of 80% methanol was added to 1 kg of degreased green tea leaves, and the mixture was extracted under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the extract obtained by concentrating the separated filtrate under reduced pressure was suspended in water, extracted five times with 1 L of ether to remove the pigment, and the aqueous layer was 1-butanol. Extracted three times with 500 mL. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain 1-butanol extract, which was dissolved in a small amount of methanol and then added to a large amount of ethyl acetate, and the resulting precipitate was dried to give 150 g of green tea leaf extract. .

Example 3 Preparation of Camperol by Acid Hydrolysis Method

20 g (v / w) of 1 N HCl-50% methanol solution (v / v) was added to 10 g of the green tea seed extract obtained in Example 1, heated to reflux for 8 hours in an 80 ° C water bath, and camellia After hydrolysis of the sugars bound to seed A and camellia seed B, the reaction solution was concentrated under reduced pressure to remove the solvent, and ethanol (200 mL) was added to the residue, followed by stirring (3 times). After removal through, the filtrate was concentrated under reduced pressure to afford the crude product. Thereafter, the obtained crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to obtain 0.95 g of camphorol.

Example 4 Preparation of Camperol by Base Hydrolysis Method

10 g of the green tea seed extract obtained in Example 1 was dissolved in dry pyridine (500 ml), and sodium methoxide (powder, 10 g) was added thereto, and the mixture was heated and refluxed in a water bath for 8 hours to give a camellia. After hydrolysis of the sugars bound to seed A and camellia seed B, the reaction solution was concentrated under reduced pressure to remove the solvent, and ethanol (200 mL) was added to the residue, followed by stirring (3 times). Removed through. The filtrate was concentrated under reduced pressure to give a crude product. Thereafter, the obtained crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to give 0.25 g of camphorol.

Example 5 Preparation of Camperol by Enzymatic Digestion Method

10 g of the green tea seed extract obtained in Example 1 was dissolved in 100 ml of 0.1 M acetic acid buffer solution (pH 4.5), and 2.5 g of enzyme (0.5 g of hesperidinase, 0.5 g of naringinase, cellulase) was added thereto. 0.5 g, β-glucuronidase 0.2 g, β-galactosidase 0.5 g, amyloglucosidase 0.3 g; manufactured by Sigma) was added and thin layer chromatography was stirred for 48 hours in a 37 ° C water bath. When the substrates (camelliaside A and camelliaside B) were completely lost, the reaction mixture was heated for 10 minutes in hot water (80-100 ° C.) to terminate the reaction. The reaction solution was concentrated under reduced pressure to remove the solvent, Ethanol (200 mL) was added and stirred (three times), and then the precipitate was removed by filtration, and the filtrate was concentrated under reduced pressure to give a crude product. Thereafter, the obtained crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to obtain 1.02 g of camphorol.

Example 6 Preparation of Camperol Using Microorganisms

10 g of the green tea leaf extract obtained in Example 2 was dissolved in 100 ml of ionized water, sterilized at 121 ° C. for 30 minutes, cooled to 30 ° C., and then pre-cultured Aspergillus niger KCCM 11885 was liquid. 5 to 10% of the inoculum was incubated for 5 days at 30 ℃, and then confirmed the scavenging rate of the substrate by thin layer chromatography to terminate the reaction when the substrate is completely lost, the culture is recovered by centrifugation at 5,000 to 10,000 rpm A precipitate was washed three times with distilled water and centrifuged to obtain a reaction solution as a precipitate. The precipitate was then stirred by adding ethanol (200 mL) (three times), and the precipitated salts were removed by filtration, and then filtered. The filtrate was concentrated under reduced pressure to give the crude product. The obtained crude product was then separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to give 0.62 g of camphorol.

Experimental Example 1 Keratinocyte Differentiation Promoting Effect

In order to examine the differentiation promoting effect of keratinocytes of camphorol obtained from Examples 3 to 6, the amount of CE (Cornified Envelop) generated during the differentiation of keratinocytes was measured using absorbance.

First, the keratinocytes of the primary cultured humans isolated from the epidermis of the newborn were put in a culture flask and adhered to the bottom, and then the test substance of Table 1 was treated at 1 ppm concentration in the culture medium. It was incubated for 5 days until it grew about%. At this time, the low calcium (0.03mM) treated group and the high calcium (1.2mM) treated group were used as negative and positive controls, respectively. Then, the cultured cells were harvested and washed with PBS (Phophate buffered saline), followed by 10 mM Tris-HCl buffer (Tris-HCl, containing 2% SDS (sodium dodecyl sulfate) and 20 mM Dithiothreitol (DTT). pH 7.4) After adding 1 ml of sonication, boiling, and centrifugation, the precipitate was again suspended in 1 ml of PBS to measure absorbance at 340 nm. Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a reference when evaluating the degree of cell differentiation. The results are shown in Table 1 below.

Test substance Differentiation capacity in keratinocytes (%) Low calcium (0.03 mM) solution (negative control) 100 High calcium (1.2 mM) solution (positive control) 210 Camperol of Example 3 142 Camperol of Example 4 137 Camperol of Example 5 140 Camperol of Example 6 143

As a result, as shown in Table 1, the camphorol obtained in Examples 3 to 6 was found to promote differentiation of keratinocytes.

Experimental Example 2 Measurement of Skin Barrier Function Recovery and Skin Moisturizing Effect in Hairless Mouse Skin

In order to measure the effect of the camphorol obtained from Examples 3 to 6 on the recovery of the damaged skin barrier function due to skin damage and the increase in skin moisturizing power, the following experiment was performed.

First, acetone is applied periodically to the back of hairless mice (Charles River, Japan) 8 to 10 weeks after birth for 5 days twice a day to induce a loss of barrier function of skin such as experimental animals, and then with an evaporimeter. Vehicle containing a mixture of propylene glycol and ethanol at a ratio of 7: 3 as a test substance for experimental animals with skin measured by TEWL (Transepidermal water loss) and having a transdermal water loss of 40 g / m ² / hr or more. A sample containing 1% each of Vehicle (vehicle treated) and each camphorol (camphorol treated group) obtained from Examples 3 to 6 was used twice a day for 3 days at a dose of 200 μl per 5 cm ² of skin area. TEWL was measured at regular intervals during continuous application and the results are shown in FIG. 1. In addition, by using a coronometer (Corneometer, Courage Khazaka, Germany) simultaneously measured the skin moisture content is shown in Figure 2 the results. At this time, the skin moisture content of the untreated group was also measured.

As a result, as shown in FIG. 1, barrier damage was recovered more rapidly in the camphorol treated groups obtained from Examples 3 to 6 than in the vehicle treated group. In addition, as shown in Figure 2, also in the skin moisture content of the camphorol treatment group obtained from Examples 3 to 6 was higher than the vehicle treatment group and the non-treatment group, it can be seen that the skin moisture content increases with the recovery of barrier damage. there was.

From the above results, camphorol obtained from green tea-derived camphorol glycosides using acid, base or enzyme or microorganisms producing this enzyme promotes the differentiation and proliferation of skin cells, the differentiation of keratinocytes, and repairs damaged skin barrier. And it was confirmed that the effect is very excellent in increasing the skin moisturizing power.

Examples of the formulation of the composition will be described below, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1 Soap Preparation

Camperol of Example 3 ... 1.00 (%)

Maintenance .....................

Sodium hydroxide ...............

Sodium Chloride ...............

Spices ..................................

The whole amount was 100 with purified water, and soap was prepared by the above blending ratio.

Formulation Example 2 Lotion Preparation

Camperol of Example 4 ...... 3.00 (%)

L-ascorbic acid-2-phosphate magnesium salt ......... 1.00

Water Soluble Collagen (1% Aqueous Solution) ................. 1.00

Sodium Citrate ............... 0.10

Citric Acid ........................... 0.05

Licorice Extract ......... 0.20

1,3-butylene glycol ............ 3.00

The whole amount was 100 with purified water, and a lotion was prepared by the above blending ratio (%).

Formulation Example 3 Cream Preparation

Camperol of Example 5 ............. 1.00 (%)

Polyethylene Glycol Monostearate ............ 2.00

Self-emulsifying glycerin monostearate ............ 5.00

Cetyl Alcohol ... 4.00

Squalene ............... 6.00

Glyceryl tri2-ethylhexanoate ......... 6.00

Sphingose Glucose Lipid ......... 1.00

1.3-Butylene Glycol ............ 7.00

The total amount was 100 with purified water, and a cream was prepared at the blending ratio (%).

Formulation Example 4 Pack Preparation

Camperol of Example 6 ............. 5.00 (%)

Polyvinyl Alcohol ........................ 13.00

L-ascorbic acid-2-phosphate magnesium salt .............. 1.00

Lauroylhydroxyproline ......... 1.00

Water Soluble Collagen (1% Aqueous Solution) ... 2.00

1,3-butylene glycol ...... 3.00

Ethanol ......................... 5.00

The total amount was 100 with purified water, and a pack was prepared at the blending ratio (%).

Formulation Example 5 Preparation of Cosmetic Solution

Camperol of Example 3 ........ 2.00 (%)

Hydroxyethylene Cellulose (2% Aqueous Solution) ......... 12.00

Xanthan gum (2% aqueous solution) ........................ 2.00

1,3-butylene glycol ............... 6.00

Concentrated Glycerin ... 4.00

Sodium hyaluronate (1% aqueous solution) ........ 5.00

The whole amount was 100 with purified water, and a cosmetic liquid was prepared at the blending ratio (%).

As described above, the camphorol according to the present invention exhibits the effect of promoting differentiation of keratinocytes, restoring damaged skin barrier function and increasing skin moisturizing power. Therefore, the external composition for skin containing camphorol is for skin moisturizing, preventing or improving skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation, and for moisturizing skin and enhancing skin barrier function. And it can be usefully used as a cosmetic composition or pharmaceutical composition for inducing skin keratinocyte differentiation.

Claims (16)

A skin external preparation composition for moisturizing skin containing camphorol as an active ingredient. Skin external preparation composition for enhancing skin barrier function containing camphorol as an active ingredient. Skin external preparation composition for inducing skin keratinocyte differentiation containing camphorol as an active ingredient. The composition for external application for skin according to any one of claims 1 to 3, wherein the camphorol has a camphorol content of 90% or more. According to any one of claims 1 to 3, wherein the camphorol is obtained by separating the camphorol from the camphorol glycoside through a decomposition reaction using an acid, a base, an enzyme, or a microorganism producing the enzyme Skin external preparation composition. 6. The composition for external application for skin according to claim 5, wherein the camphorol glycoside is derived from a plant. 7. The composition for external application for skin according to claim 6, wherein the camphorol glycoside is derived from green tea. 6. The skin according to claim 5, wherein the acid is at least one acid selected from the group consisting of hydrochloric acid, sulfuric acid and nitric acid, or a mixed solvent of these acids with at least one alcohol selected from the group consisting of ethanol, methanol and butanol. External preparation composition. The method of claim 5, wherein the base is at least one base selected from the group consisting of sodium hydroxide and potassium hydroxide or mixed solvent of these bases with at least one alcohol selected from the group consisting of ethanol, methanol and butanol Skin external preparation composition. 6. The external preparation composition for skin according to claim 5, wherein the enzyme is an exo sugar-binding degrading enzyme that separates the camphorol by removing the sugar portion from the camphorol glycoside. The method of claim 10, wherein the exo-glucose degrading enzyme is a glucosidase, arabinosidase, rhamnosidase, xylosidase, cellulase, hess Group consisting of hesperidinase, naringinase, glucuronidase, pectinase, galactosidase and amyloglucosidase Skin external composition, characterized in that at least one selected from. According to claim 5, wherein the microorganism producing the enzyme is genus Aspergillus, Bacillus, Bacillus, Penicillium, Rhizopus, Rhizomucor, At least one selected from the group consisting of genus talomyces, genus bifidobacterium, genus mortierella, genus cryptoococcus, and genus microbacterium. The external preparation composition for skin. The external preparation composition for skin according to any one of claims 1 to 3, wherein the external preparation composition for skin prevents or improves dry skin, atopic dermatitis, contact dermatitis or psoriasis caused by incomplete epidermal differentiation. The skin external composition according to any one of claims 1 to 3, wherein the external composition for skin is a cosmetic composition. The external preparation composition for skin according to any one of claims 1 to 3, wherein the external preparation composition for skin is a pharmaceutical composition. The skin external composition according to any one of claims 1 to 3, wherein the external composition for skin contains camphorol in an amount of 0.0001 to 10% by weight based on the total weight of the composition.

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