KR101888184B1 - composition comprising extract of chrysanthemum zawadskii and Aronia mandschurica - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a mixed extract consisting of perennials and aronia. The cosmetic composition according to the present invention exhibits a very excellent antimicrobial activity against gram negative bacteria, positive bacteria, yeast and mold, It is wide and has excellent preservative ability. It does not affect the stability of the formulation and hardly irritates the skin.

Description

A cosmetic composition comprising a mixture of extracts of chrysanthemum zawadskii and Aronia mandschurica,

The present invention relates to a cosmetic composition containing a mixed extract comprising Rhododendron and Aronia.

In recent years since 1990, in the development of physiologically active substances, other fields of medicine and physiological activity such as immunity, cardiovascular and the like have been studied more important than anticancer and antibacterial fields. The antimicrobial field, which was considered to be the most important in the past, decreased in importance in the late 1980s. In addition, in the case of antibiotics based on β-lactam, which is most frequently used in antimicrobial drugs, the tolerance has increased to a very rapid trend, and it is reported that the overall tolerance rate reaches about 80% in Korea at present. Most of the antifungal agents used for clinical use are azole or allyamine, and both of these antifungal agents have generated many resistant strains over time.

Accordingly, there is a growing interest in the problem of tolerance of antimicrobial agents in the world, and development of new antimicrobial materials that can overcome resistance problems is urgently required. In addition, not only resistance but also pharmacological efficacy will require the discovery of new targets for antimicrobials and the development of specific antimicrobials for new targets.

Antimicrobial activity of alkaloids, flavonoids, phytoalexins, antimicrobial peptides, and organic acids and fatty acids has been reported in studies on natural antimicrobial substances. It is presumed that this is due to the effect of chelate (EI-Shenawy, MA et al., J. Food Protec., 1989, 52 (11), 771-778) (Bizri, JN et al., J. Food Science, 1994 , 59 (1), 130-135).

However, some of the natural antimicrobial raw materials such as spices and herbal medicines have a characteristic of being too dark in color and strong in raw material picking, so that they are limited or unsuitable for use in cosmetics requiring a beautiful product. In the case of some raw materials, The viscosity is lowered and it is difficult to use it as a cosmetic raw material because it affects the emulsification system. In addition, the antimicrobial active ingredient contained in the plant extract has a narrower antimicrobial spectrum than the chemical preservative, and the physicochemical stability against heat, light, and oxygen is very low as compared with the chemical preservative, so that the antimicrobial active ingredient It has a problem that it can not provide enough preservative activity when it is decomposed and actually used by a consumer. Therefore, most of them are not commercialized due to limitations as a natural substance.

HNOKITOL, Megonol, and Grapefruit Seed Extract (DF-100) were developed as commercial antibacterial agents. However, most of them were economical, antibacterial spectrum, restriction of use range Due to problems, more advanced natural antimicrobial agents are needed.

KR 10-2009-0007079 A, January 16, 2009

 Bizri, JN et al., J. Food Science, 1994, 59 (1), 130-135

Accordingly, an object of the present invention is to solve the problems as raw materials for cosmetics such as existing natural antimicrobial agents, and exhibit excellent antimicrobial activity against gram-negative bacteria, gram-positive bacteria, fungi including fungi and yeast, Which is excellent in antiseptic ability, has little influence on the stability of the formulation and is free from skin irritation.

In order to achieve the above object, the present invention provides a cosmetic composition comprising 0.01 to 30% by weight based on the total weight of the composition of Rhododendron japonica extract and Arrnoia mixed extract to produce gram-negative bacterium, positive bacterium, Which is excellent in antiseptic ability due to its broad antibacterial spectrum, does not affect the stability of the formulation and hardly irritates the skin.

Hereinafter, the present invention will be described in detail.

Gujeolcho according to the invention and Chokeberry (Aronia melanocarpa ) mixed extracts have an effect of further enhancing the antimicrobial activity against Gram negative bacteria, positive bacteria, yeast and mold, as compared with individual use.

Chrysanthemum zawadskii var latilobum is a perennial plant of Asteraceae. It can be used as a medicinal product after it is dried in the sun by picking flower ear and leaf stem at the beginning of late autumn flower bloom. Flower ears and hay can be used for pneumonia, bronchitis, cough cold, pharyngitis, headache, or hypertension, and can also be used for the treatment of indigestion or gastrointestinal disorders.

Aronia ( Aronia melanocarpa ) is a berries fruit belonging to the Rosaceases family Rosaceae, also known as Black Chokeberry or Black Rowanberry. Originally, it grows in harsh environments such as a cold of 20 degrees in the North America region and intense ultraviolet rays for five months. The flowers last 11 ~ 20 days, such as corn oil, plum, and cherry, and bear red berries from September to November. The fruit has a good taste and aroma, and the juice is used as a drink. Aronia can usually harvest about 10 to 15 kg of fruit per plant.

The present invention provides a cosmetic composition containing a mixture of Rhodiola and Rhodiola as an active ingredient.

In the present invention, the mixed extract may be prepared by mixing the herbicide extract obtained by mixing the herbicide with boiling distilled water, and a herbicidal extract obtained by mixing the herbicidal extract with water, a C1-C4 lower alcohol, glycerol, propylene glycol, 1,3-butylene glycol, ethyl acetate , Benzene, hexane, diethyl ether and dichloromethane are mixed in the same volume ratio in the same amount.

Further, in the present invention, the aronia is characterized in that the extraction solvent is methanol or a mixed solvent of methanol and water.

More specifically, the perennial herb may be mixed with distilled water, for example, mixed at a ratio of 1: 5, for example, about 2 hours at about 100 ° C, and pressed to obtain a perennial extract.

Aronia can be obtained by mixing with an organic solvent, for example, methanol. The methanol may be about 50 to 100%, more specifically, about 80% of methanol may be used. The Aronia extract can be obtained by mixing the aronia with an organic solvent in a ratio of, for example, 1:10, and shaking and pressing, for example, at 25 캜 for about one week at 150 rpm.

Then, the Rhizoma extract and the Aronia extract are mixed. The mixing of the extracts may use, for example, a magnetic hot plate and a magnetic bar.

In the present invention, the composition is characterized in that the Rhizoma extract and the Rhodiola extract are mixed in an equal volume ratio as an active ingredient, and then the mixed extract is concentrated.

In the present invention, the concentration may be performed by evaporating the solvent of the mixed extract and eluting the dried mixed extract with an organic solvent, for example, ethyl alcohol to obtain a natural concentrated mixture.

The composition according to the present invention should be used as an extract obtained by extracting the perennial herbs and the Aronia with the extraction method described above. Also, the extracts of the perennial herbs and the Aronia extracted by the above method should be mixed in the same volume ratio to obtain Gram-positive bacteria, It was confirmed that there is a synergy effect of antibacterial effect on yeast and mold.

In the present invention, it is preferable that the mixed extract of Rhododendron and Rhodiaceae is contained in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition, and when it is used in an amount of less than 0.01% by weight, If it is used in an amount exceeding 30% by weight, it affects the stability of the product and the product.

More preferably, the cosmetic composition of the present invention contains 0.1 to 10% by weight, more preferably 0.5 to 5% by weight of the extract.

The cosmetic composition containing the extract may be prepared in any form conventionally produced in the art, and examples thereof include softening lotion, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, Foams, cleansing water, powders, essences, packs, and the like, and they can be produced according to the purpose of formulation or use of the cosmetic.

The cosmetic composition according to the present invention has excellent antimicrobial activity against Gram negative bacteria, positive bacteria, yeast and mold, broad antimicrobial spectrum, excellent antiseptic ability, little effect on the stability of the formulation and little skin irritation have.

The present invention will be described in more detail with reference to the following examples. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited by these examples in any sense.

Hereinafter, the technical and scientific terms used herein will be understood by those skilled in the art without departing from the scope of the present invention. Descriptions of known functions and configurations that may be unnecessarily blurred are omitted.

[Preparation Example 1] Preparation of Rhizoma extract (water extract)

100 g of Rhus verniciflua was added to 5 L of distilled water and sealed, and the mixture was melted at 100 DEG C for 2 hours and compressed to obtain Rhus javanica extract.

[Production Example 2] Preparation of Rhizoma extract (ethanol extract)

100 g of 100% ethanol was placed in 100 ml of ethanol and sealed, and the mixture was shaken at 25 DEG C and 150 rpm for one week in a shaker to obtain a Rhizoma extract.

[Production Example 3] Preparation of Rhizoma extract (reflux cooled extract)

10 g of distilled water was added to 100 g of Korean persimmon, and the mixture was refluxed for 3 hours at 85 ° C. The supernatant was separated from the precipitate and extracted three times. Each extract was centrifuged, filtered, concentrated, and lyophilized.

[Production Example 4] Preparation of an extract of Aronia (methanol extract)

1 liter of 80% methanol was added to 100 g of dried and pulverized Aronia melanocarpa , and the mixture was sealed. The mixture was shaken at 25 DEG C and 150 rpm for one week in a shaker, and filtered to obtain a pure extract. Lornia extract was obtained.

[Preparation Example 5] Preparation of an extract of Aronia (methanol + water extract)

200 g of 80% methanol was added to 100 g of dried and crushed Aruonia, and the mixture was extracted with cold water three times each for one week. The extract was concentrated under reduced pressure to obtain a methanol extract.

[ Manufacturing example  6] Aronia  Extract preparation ( Reflux cooling  extract)

10 g of distilled water was added to 100 g of Aronia and the mixture was refluxed and cooled at 85 ° C for 3 hours. The supernatant and the precipitate were separated and extracted three times. Each extract was centrifuged, filtered, concentrated, and lyophilized.

[ Manufacturing example  7] Aronia  Extract preparation (water extract)

100 g of Aronia was put in 5 L of distilled water and sealed, and the mixture was weighed at 100 DEG C for 2 hours and pressed to obtain an Aronia extract.

[ Manufacturing example  8] Aronia  Extract preparation (ultra high pressure extract)

100 g of Aronia was placed in a vacuum, and 900 g of water was added thereto, followed by vacuum treatment using genuine air (IS-100, Namwoong, Korea). After that, it was subjected to ultra-high pressure treatment in a double liquid phase for 1 hour at 100 MPa (1,000 bar), 25 ° C, and water (buffer solvent) using an ultrahigh pressure apparatus (TFS-2L, TOYO KOATSU CO., LTD. After the ultrahigh pressure treatment was completed, the extract was centrifuged at 8,000 MPa and 15 ° C for 20 minutes using a centrifuge (Supra 22K, Hanil, Korea) to obtain an extract of Aronia.

[ Example  One]

The combined extracts obtained by mixing the Rhizoma extract of Preparation Example 1 and the Aronia extract of Preparation Example 4 in the same volume were concentrated using a rotary vacuum evaporator, followed by lyophilization, followed by extraction with ethyl alcohol, 65 g of an extract was obtained.

[ Example  2]

The combined extracts obtained by mixing the Rhizoma extract of Preparation Example 1 and the Aronia extract of Preparation Example 5 in the same volume were concentrated using a rotary vacuum evaporator, lyophilized, and then extracted with ethyl alcohol to prepare a mixture of rhizome and aronia 65 g of an extract was obtained.

[ Comparative Example  One]

The combined extracts obtained by mixing the Rhizoma extract of Preparation Example 1 and the Aronia extract of Preparation Example 6 in the same volume were concentrated using a rotary vacuum evaporator, lyophilized and then extracted with ethyl alcohol, 65 g of an extract was obtained.

[ Comparative Example  2]

The combined extracts obtained by mixing the Rhizoma extract of Preparation Example 1 and the Aronia extract of Preparation Example 7 in the same volume were concentrated using a rotary vacuum evaporator, lyophilized and then extracted with ethyl alcohol, 65 g of an extract was obtained.

[ Comparative Example  3]

The combined extracts of the Rhizoma extract of Preparation Example 1 and the Aronia extract of Preparation Example 8 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of a mixture of Rhizoma extract and Aronia.

[ Comparative Example  4]

The combined extracts of the Rhizoma extract of Preparation Example 2 and the Aronia extract of Preparation Example 4 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  5]

The combined extracts of the Rhizoma extract of Preparation Example 2 and the Aronia extract of Preparation Example 6 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  6]

The combined extracts of the Rhizoma extract of Preparation Example 2 and the Aronia extract of Preparation Example 7 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  7]

The combined extracts of the Rhizoma extract of Preparation Example 2 and the Aronia extract of Preparation Example 8 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  8]

The combined extracts of the Rhizoma extract of Preparation Example 3 and the Aronia extract of Preparation Example 4 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  9]

The combined extracts of the Rhizoma extract of Preparation Example 3 and the Aronia extract of Preparation Example 5 were concentrated using a rotary vacuum evaporator and then lyophilized and then extracted with ethyl alcohol to obtain Rhizoma extract 65 g of mixed extract was obtained.

[ Comparative Example  10]

The combined extracts of the Rhizoma extract of Preparation Example 3 and the Aronia extract of Preparation Example 6 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  11]

The combined extracts of the Rhizoma extract of Preparation Example 3 and the Aronia extract of Preparation Example 7 were concentrated using a rotary vacuum evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Comparative Example  12]

The combined extracts of the Rhizoma extract of Preparation Example 3 and the Aronia extract of Preparation Example 8 were concentrated using a rotary evaporator and then lyophilized to obtain 65 g of Rhizoma extract and Rhizoma extract.

[ Experimental Example  1] Antimicrobial activity test

In order to investigate the antibacterial activity of the embodiments 1 to 2 and Comparative Examples 1 to 12 of the mixed extract prepared above, Staphylococcus aureus (Staphylococcus aureus ATCC 6538P), Escherichia coli coli ATCC 8739) and Pseudomonas aeruginosa ATCC 9027), yeasts such as Candida albicans ATCC 10231), and Kirby-Bauer test for Aspergillus niger ATCC 22343 as a fungus . Specifically, each bacterial culture was inoculated on a Mueller-Hinton agar medium, and 100 쨉 l of each extract was dispensed per paper disc, dried and placed on a culture medium. The antimicrobial activity was observed by the inhibitory ring around each paper disk, and the results are shown in Table 1 below.

In the following table, '+' indicates antibacterial effect, and '+' indicates the degree of antibacterial activity. That is, '++' means that the antimicrobial activity is stronger than that of '+'. In addition, '±' means that the antimicrobial activity is weaker than that of '+', and '-' means that the antimicrobial activity is not observed.

As shown in Table 1, it was confirmed that the diameter of the inhibitory rings of the mixed extracts of Examples 1 and 2 according to the present invention was larger than that of the inhibitory rings of the mixed extracts of Comparative Examples 1 to 12.

The above results indicate that the mixed extracts of Examples 1 and 2 of the present invention, that is, the Rhizoma extract extracted from the mixture of distilled water and boiling distilled water, and the mixed extract of Aronia extract obtained by mixing Aronia with methanol or a mixed solvent of methanol and water It can be said that it means that the antibacterial ability is superior to the respective natural extracts or the mixed extracts extracted and mixed by the method different from the present invention.

[Experimental Example 2] Skin patch test (patch test)

The skin patch test was conducted to examine the skin irritation of the mixed extract of the example of the present invention. Using the Examples of Example 1 and Example 2 and Comparative Example 1 and Comparative Example 9, which had excellent antibacterial activity from the Experimental Example 1, 20 subjects (mean age 29 years, age range 23 to 39 years) Were applied to the skin patch test. However, those who are taking psoriasis, eczema, other skin lesions, pregnancy, breastfeeding or contraceptive, and antihistamines are excluded from this experiment.

First, the test site was wiped with 70% ethanol, dried, and 15 μg of the prepared test substance was dripped into a Finn chamber (Finx chamber, 100 × 10, EPITEST, Finland). After 24 hours of application and removal of the patches, the test area was marked with a pen. After the labeling, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) after 1 hour and 24 hours, respectively, to observe erythema and edema.

The skin response was determined according to the rules of the International Contact Dermatitis Research Group (ICDRG), and the mean skin response was calculated according to the following formula and the results are shown in Table 3 below.

As can be seen from the results of Table 3 above, the cosmetic composition containing Examples (1) and (2) according to the present invention showed almost no irritation to the skin, unlike the cosmetic compositions containing Comparative Examples (1 and 9) I could confirm.

[Formulation Example 1] Softening lotion (skin lotion)

The number of softening times was calculated according to a conventional method in the composition shown in Table 4 below.

Claims (7)

The present invention relates to a cosmetic composition comprising, as an active ingredient, a mixed extract of Rhododendron japonica extract and Aronia melanocarpa extract,
The Rhizoma extract is extracted with boiling water,
Wherein the Aronia extract is obtained by extracting Aronia with a methanol solvent or a mixed solvent of methanol and water.
Cosmetic composition. delete delete The method according to claim 1,
Wherein the mixed extract is contained in an amount of 0.01 to 30% by weight based on the total weight of the cosmetic composition. 5. The method according to any one of claims 1 and 4,
The composition is prepared by mixing the Rhizoma extract and Rhizoma extract with an equivalent volume ratio,
Wherein the mixed extract is concentrated. 6. The method of claim 5,
The concentration
The solvent of the mixed extract was evaporated to dryness,
Wherein the dried mixed extract is eluted with an organic solvent. The method according to claim 6,
Wherein the organic solvent is ethyl alcohol.