KR101013203B1 - An antibacterial composition and a method for preparing the same - Google Patents

Abstract

Antimicrobial compositions and methods of making the same are provided. The antimicrobial composition includes Gujeolcho, Buddha Son, Eoseongcho, Hwanggyeongpi, Ginseng, Dandelion, Prunus bark, Honeysuckle, Triticale, and Pasqueflower extract.

Folk medicine, antibacterial activity, MRSA

Description

An antibacterial composition and a method for preparing the same

The present invention relates to an antimicrobial composition and a method for producing the same, and more particularly, to an antimicrobial composition and a method for producing the same using a natural substance.

In recent years since 1990, in the development of bioactive substances, other fields of biomedical activity such as immunity and cardiovascular are more important than anticancer and antibacterial fields. The antimicrobial field, which was considered as the most important in the past, has become less important for a while in the late 1980s, but the importance of thorns is increasing with the recent increase in infections and infections by resistant bacteria. In addition, in the case of the beta-lactam (β-lactam) antibiotics most commonly used in antimicrobial drugs, the resistance is increasing at a very rapid trend, and currently, it is reported that the overall resistance rate is about 80% in Korea. Most antifungal agents used for clinical use are azole or allyamine based, and the two antifungal agents are very resistant to strains over time.

Therefore, there is an increasing global interest in the problem of resistance of antimicrobial agents, and the development of new antimicrobial material that can overcome the resistance problem is urgently required. In addition, in terms of resistance as well as pharmacological efficacy, the discovery of new targets for antimicrobial agents will be necessary and the development of antimicrobial materials specific for new targets will also be needed.

The problem to be solved by the present invention is to provide an antimicrobial composition having an increased antimicrobial activity and excellent stability.

Another problem to be solved by the present invention is to provide a method for producing an antimicrobial composition having an increased antimicrobial activity and excellent stability.

The antimicrobial composition according to an embodiment of the present invention for achieving the above object includes Gujeolcho, Buddha's hand, Eoseongcho, Hwang Jingpi, Gosam, Dandelion, Prunus bark, Honeysuckle, Triticale, and Pasqueflower extract.

Method for producing an antimicrobial composition according to an embodiment of the present invention for achieving the above another object is to obtain each extract from Gujeolcho, Buddha Buddha, Eoseongcho, Hwanggyeongpi, red ginseng, dandelion, cherry bark, honeysuckle, triticale, and pasqueflower And mixing the respective extracts.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms, and only the present embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the art to which the present invention pertains. It is provided to fully convey the scope of the invention to those skilled in the art, and the present invention is defined only by the scope of the claims. Accordingly, well known techniques in some embodiments are not described in detail in order to avoid obscuring the present invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. As used in this specification, inclusions and / or comprisings are used in the sense that they do not exclude the presence or addition of one or more other elements, steps, other than the components mentioned.

Hereinafter, an antimicrobial composition according to some embodiments of the present invention will be described.

The antimicrobial composition according to some embodiments of the present invention comprises a natural mixture comprising Gujeolcho, Buddha Son, Eochocho, Hwanggyeongpi, Red Ginseng, Dandelion, Prunus bark, Honeysuckle, Triticale, and Pasqueflower Extract.

More specifically, the natural mixture may be composed of a mixture of Gujeolcho, Buddha's hand, Eochocho, Hwangkyeongpi, Ginseng, Dandelion, Prunus bark, Honeysuckle Flower, Trichophyllum and Pasqueflower Extract obtained separately.

In addition, the natural mixture may be a natural concentrated mixture concentrated a mixture of Gujeolcho, Buddha Buddha, Eoseongcho, Hwanggyeongpi, Korean ginseng, dandelion, cherry bark, honeysuckle, triticale, and pasqueflower extract.

The Gujeolcho, Buddha Son, Eoseongcho, Hwanggyeongpi, red ginseng, dandelion, cherry bark, honeysuckle, triticale, and palebloom extract may be, for example, 5 to 15% by volume, but is not limited to the above values, Of course it can be made up by volume.

Antimicrobial compositions in accordance with some embodiments may be considered as alternative medicine by obtaining from herbs commonly used around life, or traditionally delivered folk remedies. In addition, as shown in the experimental example to be described later, the antimicrobial ability is increased more than the case of using the natural extracts individually, and also to maintain the antibacterial ability against staphylococcus aureus resistant to beta-lactam antibiotics. It has a much better effect.

The method for preparing an antimicrobial composition according to some embodiments of the present invention obtains each extract from Gujeolcho, Buddha's Son, Eochocho, Hwanggyeongpi, Ginseng, Dandelion, Prunus bark, Honeysuckle, Trichophyllum and Pasqueflower, and each of the extracts. It involves mixing.

First, each extract is obtained from Gujeolcho, Buddhason, Eoseongcho, Hwanggyeongpi, Ginseng, Dandelion, Prunus bark, Honeysuckle Flower, Triticale, and Pasqueflower. Each extract may be extracted using distilled water or an organic solvent to suit the properties.

More specifically, Gujeolcho, Buddha-son, Eochocho, hwangpi skin, Gosam, Dandelion, and Prunus bark are individually mixed with distilled water, for example in a ratio of 1: 5, for example at about 100 ° C. Each extract can be obtained by decoction and compression for about an hour.

Honeysuckle, triticale, pasqueflower and the like can be obtained by mixing with an organic solvent such as ethyl alcohol. In this case, the ethyl alcohol may be about 20% to about 100%, more specifically 100% of ethyl alcohol may be used. The extracts may be obtained by mixing the phosphorus flower, triticale, pasqueflower and the like with an organic solvent, for example, in a ratio of 1: 5, for example, shaking and compressing at about 25 ° C. and about 150 rpm for about a week. .

Subsequently, the Gujeolcho, Buddhason, Eoseongcho, hwangpi skin, red ginseng, dandelion, cherry bark, honeysuckle, triticale, and pasqueflower extract are mixed. Mixing the extracts may use, for example, magnetic hot plates and magnetic bars.

Furthermore, the mixed extracts can be concentrated to dryness using an evaporator and eluted with an organic solvent, for example ethyl alcohol, to obtain a natural concentrated mixture.

A more detailed description of the present invention will be described through the following specific experimental examples, and details not described herein will be omitted since those skilled in the art can sufficiently infer technically. However, the following experimental examples are provided to illustrate the present invention, and the present invention is not limited by the experimental examples.

Experimental Example  1-collection of natural materials

Natural materials were collected around folk remedies that are said to be effective in infectious diseases in Korea. Collected folk remedies include:

(1) Chrysanthemum zawadskii var . latilobum ) is a perennial herb of the Asteraceae family, which can be harvested and dried in the sun after harvesting flower ear and leaf stems at the beginning of flowering in late autumn. Flower ear and hay can be medicinal for pneumonia, bronchitis, cough cold, pharyngitis, headache, or high blood pressure, and can also be used for the treatment of indigestion or gastrointestinal diseases.

(2) Selaginella tamariscina ) is an evergreen perennial grass with the Buddha's hand. It grows on dry rocks and grows blue when it rains, and when it is wet, the leaves are dried like a ball. Chronic hepatitis, cirrhosis, jaundice, cough, kidney stones, schizophrenia, various types of cancer, bronchitis, pneumonia, tonsillitis are also effective. In addition, elderly people are said to be energetic when they eat the Buddha's hand when they are weak and have a weak body, and are also known as plants having excellent anticancer effects.

(3) 300 seconds ( Saururus chinensis ( Lour ) Bail ) is a perennial perennial grass about 30-90cm high, root is white, stem is straight, and leaf shape is pointed egg-shaped. Three hundred seconds is an unusual plant that grows green in early summer, turns three leaves under the flower white, and then returns to green. It is effective in eliminating constipation and stool, and is effective in various gynecological diseases. It is used for the treatment of cold chills, uterine inflammation, menstrual irregularities and uterine escape. It also eliminates inflammation and has strong anticancer activity. Three hundred seconds of the roots, leaves, stems, flowers are used as medicine, decoction by tea, or soaked in alcohol can eat.

(4) Taraxacum platycarpum ) was used as an important drug not only in Korea but also in Indians of China, Japan, India, Europe and America. It is a herb that is effective in various diseases so that there are few records of dandelion in many places of the old book. For gastroenteritis such as gastritis, gastric ulcer, chronic hepatitis, liver disease such as fatty liver, and chronic enteritis, for example, it is known that the dandelion leaves can be washed and chewed, or they can be eaten by being thickly rooted and dried in the shade. It is also said to be effective against asthma and cough.

(5) Prunus serrulata var . spontanea ( Max .) Wils ) is a deciduous tree of the Rosaceae family . It is used as a cough medicine called 'protein' by extracting a substance called ' sakuranin ' contained in cherry bark. It does not hurt.

Seawater, cough is known to be effective in taking the cherry bark thickly, and the bark of the cherry tree is said to be effective in food poisoning, fish poisoning, mushroom poisoning, etc. For indigestion or diarrhea, peel off the green skin of a young eggplant, about 3 to 5 cm in diameter, cut it finely, dry it well in the shade, and then eat it by hanging it. In addition, the cherry leaves are also effective for skin diseases, it is known to be effective when applied to the sweat bands, eczema, skin diseases by attaching the dried cherry leaves in the shade.

(6) Lonicera japonica Thunb ) is a semi-evergreen vine plant of the genus Phloxaceae, which blooms two flowers in each node in early summer, and the color of the flowers turns white over time and is called "gold silver". Indongcho is cold and tastes sweet and slightly bitter, very urine, inflammatory, and disinfecting. It is known to be effective in various carbuncles, swellings, scabies, dysentery, fever, and playing windows, and is effective in various inflammatory diseases such as colitis, gastric ulcer, cystitis, pharyngitis, tonsillitis and conjunctivitis.

Honeysuckle flowers can be picked up just before the blossoming and dried in the shade. Leaves and stems can be cut in autumn and dried in the shade. Indong is soaked in alcohol and the effect is faster. After picking up white flowers that bloomed in early summer, they are dried in about 1.8 liters of liquor and about 100g of artificial flowers are ripened in a warm place for a month. It is said to be effective for various boils, swellings, each, syphilis and arthritis.

(7) Houttuynia cordata ) is a perennial grass growing in the shadow of the mountains. It grows in southern Korea, Jeju Island, and Ulleungdo, and is known as a toxic herb. In the sense that fish and fishy smells from the stem and leaves is called Seongchocho (魚腥草), antibacterial and detoxification is known to have anti-cancer action. In addition, it is known to be effective in treating inflammation of the bladder, urethra, uterus, fistula, swelling, redness, acne, pneumonia, otitis media, sinusitis and the like. Eoseongcho about 20 to 30g to 500cc of water until 300cc is divided into three times a day to drink.

(8) sake greens ( Paris verticillata ) is a perennial herb that is effective for asthma, boils and chronic bronchitis, and is known to be used for traumatic bleeding and hemolytic pain.

(9) Ginkgo biloba is a fruit of ginkgo biloba, which is known to have effects such as Jinhae, sputum, etc. It is also taken as a nourishing drug.

(10) Bidens tripartite ) is an annual plant of the Asteraceae family, which is often grown in rice fields or streams. Used to treat tuberculosis, young sprouts are edible.

(11) Pulsatilla cernua var . koreana ) is a perennial herb of the buttercup family, covered with soft and thin hairs all over, with long, straight and dark brown roots. Pasqueflower is not only good for abdominal pain, but is also used as a medicine for headache, swelling, dysentery, heart disease, malaria, gastritis, etc. In particular, it is known to be effective in treating brain diseases. Put about 500g of pasqueflower leaves in about 3 liters of water, and cut it into half. Put a bowl of decoction water and glutinous rice in a jar and cover it for 10 days. Eat this drink three times a day, one glass before meals.

(12) Songjin (松 津) is a secretion produced when a pine tree is damaged. It is known to make new flesh, stop pain, and have strong sterilization. To use it as a medicine, cut pine tree bark into the water, boil it down, boil it, filter it with a cloth, and put it in cold water. Used as an external medicine for eczema, burns and scabies. This is known because the essential oil component of rosin has skin irritation, antibacterial action and bactericidal action.

(13) White is Bletilla striata (Thunberg) Reichenbach fil.) is a dried herb. The white is odorless and tastes a bit bitter and mucus, while the weakness is bitter and sweet and cool. It is used as a hemostatic drug used for hemostasis caused by lung fever and hemorrhage caused by stomach fever. In trauma, powder is applied to affected areas. It is also used for surgical diseases such as boils and is known to have the effect of boosting new flesh. In addition, local bleeding hemostasis, stomach, duodenum treatment, fungi, blood pressure increase, anticancer activity has been reported.

(14) Betula platyphylla var . japonica ) is a deciduous arborescent and grows in deep mountain suns. Birch bark, also called baekhwapi (白樺 皮) is known to be used for diuresis, pain, fever.

(15) Hemerocallis fulva ) is a perennial herb, also known as a fern. In addition to nutrients such as protein, glucose, fat, pollen, vitamins, and minerals, cones contain adenine, corinine, and arginine, which are effective in treating diuresis, fever, antitussives, and pain, and for the treatment of anemia and boils. Dried flowers are known to be good for nourishing tonic and fatigue when dipped in alcohol.

(16) Phellodendron amurense is a deciduous arboreous tree 10m in height and its bark is light gray. It is known to have bile secretion and anti-inflammatory effects. Berberine, an alkaloid in the cortex, is known to have antibacterial, sedative, and analgesic effects on pathogenic microorganisms, including Escherichia coli and erythrocytes. . Bacterial dysentery, diarrhea, oral ulcers, arthralgia, etc., 10g of the dermis and decoction three times a day is said to be effective.

(17) Sophora flavescens grows in sunny grasses with perennial grasses. Boiled red ginseng roots and chronic hepatitis is known to be effective.

(18) Liriope platyphylla ) is a perennial herbaceous plant that grows mainly in the shade. Macmundong is said to be effective in strengthening the lungs, invigorating and preventing coughing and asthma. It is also effective in Jinhae, mind and body anxiety, expectoration and nourishing tonic.

Experimental Example  2-extraction of natural substances

Among the folk medicines collected as described above, drugs that are consumed in water are extracted with distilled water, and drugs that are soaked in alcohol are extracted with ethyl alcohol. Specifically, 10 g of gujeolcho was sealed in 50 ml of distilled water, and sealed at 2O < 0 > C for 2 hours to obtain gujeolcho extract. In the same manner for each of Buddha hand, dandelion, cherry bark, eoseongcho, yellow cortex, and red ginseng, each extract was obtained.

The extract of three hundred seconds was obtained by putting 10 g of three hundred seconds into 50 ml of 100% ethyl alcohol and sealing, shaking for 1 week at 25 ℃, 150rpm in a shaker. In the same manner, respectively, a honeysuckle and pasqueflower extract were obtained.

Cherry leaves, honeysuckle, honeysuckle, saponflower, ginkgo, ginkgo biloba, cauliflower, rosin, baekbaek, birch, cone stem, mackmundong, etc. were individually sealed by 10g each for 50ml of distilled water and 50ml of ethyl alcohol. Subsequently, the material mixed with distilled water was pressed for 2 hours at 100 ° C., and the material mixed with ethyl alcohol was shaken for 1 week at 25 ° C. and 150 rpm in a shaker.

Experimental Example  3-antibacterial assay

In order to assay the antimicrobial effect of each extract obtained in Experimental Example 2, Kirby-Bauer test was performed on 22 bacteria including S. aureus, which are stored frozen in a clinical pathology department of Masan University microbiology laboratory. Specifically, each bacterial culture was inoculated on Müller-Hinton agar medium, 100 μl of each extract per paper disk was dispensed and dried and placed on the medium. The antimicrobial activity was observed with the inhibitory rings formed around each paper disk, and the results are shown in Table 1. The strain names used in this experiment were as follows.

a. Staphylococcus aureus (Gram positive cocci)

b. Shigella sonnei (Gram negative bacilli)

c. Staphylococcus xylosus (Gram positive cocci)

d. Klebsiella pneumonia (Gram negative bacilli)

e. Proteus vulgaris (Gram negative bacilli)

f. Morganella morganii (Gram negative bacilli)

g. Streptococcus agalactiae (Gram positive cocci)

h. Streptococcus pyogenes (Gram positive cocci)

i. Salmonella paratyphi B (Gram negative bacilli)

j. Salmonella enterica serovar Typhi (Gram negative bacilli)

k. Alcaligenes xylosoxidans (Gram negative bacilli)

l. Enterobacter arengenes (Gram negative bacilli)

m. Enterobacter cloacae (Gram negative bacilli)

n. Pseudomonas aeruginosa (Gram negative bacilli)

o. Escherichia coli O 157 (Gram negative bacilli)

p. Escherichia coli (Gram negative bacilli)

q. Serratia marcescens (Gram negative bacilli)

r. Enterobacter faecalis (Gram positive cocci)

s. Stenotrophomonas maltophilia (Gram negative bacilli)

t. Bacillus cereus (Gram positive bacilli)

u. Corenebacterium spp . Gram positive bacilli

v. Bacillus licheniformis (Gram positive bacilli)

TABLE 1

Strain a b c d e f g h i j k l m n o p q r s t u v Gujeolcho + - - - - - - - - - - - - - - - - - - - - - Buddha hand + - + - - - - - - - - - - - - - - - - - - - Three hundred seconds + - + - - - - - - - - - - - - - - - - - - - dandelion + - + - - - - - - - - - - - - - - - - - - - Cherry bark + - + - - - - - - - - - - - - - - - - - - - Cherry tree leaves - - - - - - - - - - - - - - - - - - - - - - Honeysuckle - - - - - - - - - - - - - - - - - - - - - - Honeysuckle + - + - - - - - - - - - - - - - - - - - - - Honeysuckle leaf - - + - - - - - - - - - - - - - - - - - - - Eoseongcho + - + - - - - - - - - - - - - - - - - - - - Spat greens - - - - - - - - - - - - - - - - - - - - - - Bank - - - - - - - - - - - - - - - - - - - - - - Ginkgo leaves - - - - - - - - - - - - - - - - - - - - - - Clogs - - - - - - - - - - - - - - - - - - - - - - pasqueflower + - - - - - - - - - - - - - - - - - - - - - Rosin - - + + - - - - - - - - - - - - - - - - - - White - - - + - - - - - - - - - - - - - - - - - - Birch Trees - - - - - - - - - - - - - - - - - - - - - - Conical stem - - + - - - - - - - - - - - - - - - - - - - Yellow cortex (skin) + - + - - - - - - - - - - - - - - - - - - - Gosam + - - - - - - - - - - - - - - - - - - - - - Macmundong - - - - - - - - - - - - - - - - - - - - - -

Looking at Table 1, each natural extract showed some effects against Gram-positive bacteria, in particular S. aureus and pneumococci. In the above table, + represents the case of showing the antimicrobial activity for each strain,-represents the case of not.

Experimental Example  4-Preparation of Antimicrobial Compositions

From the results of Experimental Example 3, 25 to 30 ml of Gujeolcho, Buddha's hand, three hundred seconds, dandelion, cherry bark, honeysuckle, Echocho, pasqueflower, hwangjupi, and red ginseng extract showing antibacterial activity against S. aureus in 500 ml beaker And mixed for about 20 minutes using a magnetic bar on a magnetic hot plate. The mixture of extracts was dried using an evaporator until the solvent was completely evaporated. Subsequently, the concentrated and dried mixture was poured into 20 ml of 100% ethyl alcohol and eluted to obtain a natural concentrated mixture.

Experimental Example  5-Antibacterial assay of natural concentrated mixtures

In order to confirm the antimicrobial activity of the natural concentrated mixture obtained in Experimental Example 4, the Kirby-Bauer method carried out in Experimental Example 3 was performed. Specifically, Gujeolcho, Buddha's hand, 300 seconds, Dandelion, cherry bark, honeysuckle, Echochome, Pasqueflower, Hwangkyungpi, and Ginseng on Müller-Hinton Agar medium cultured with Methicillin Resistance Staphylococcus Aureus (MRSA) A paper disk was prepared by dispensing 100 µl of the extract alone and drying, and a paper disk obtained by dispensing 100 µl of the natural concentrated mixture. The antimicrobial activity was observed based on the inhibitory rings generated around each paper disk, and the results are shown in Table 2 and FIGS. 1 to 4. 1 to 4 are photographs showing the antimicrobial activity assay for MRSA of Gujeolcho, Buddha's hand, honeysuckle, pasqueflower, and natural concentrated mixture using Kirby-Bauer method.

TABLE 2

Natural concentrated mixture Gujeolcho Buddha hand Three hundred seconds dandelion Cherry bark Honeysuckle Eoseongcho pasqueflower Yellow cortex Gosam Antimicrobial activity ++ + + ± ± ± ± ± ± + ±

Looking at the Table 2 and Figures 1 to 4, it can be seen that the diameter of the inhibitory ring of the natural concentrated mixture is larger than that of the inhibitory ring of each extract. This can be said that the natural concentrated mixture is superior to each extract antibacterial ability. That is, it can be seen that the antimicrobial activity of the natural concentrated mixture is further increased. At this time, '+' indicates that there is an antimicrobial effect, the number of '+' indicates the degree of antimicrobial activity. In other words, '++' means that the antibacterial activity is stronger than that of '+'. In addition, '±' means that the antimicrobial activity is weaker than the case of '+'.

In addition, since MRSA is one of the staphylococcus aureus resistant to beta-lactam antibiotics, the fact that the natural concentrated mixture has better antimicrobial activity than that of each extract against MRSA indicates that the antimicrobial activity of the natural concentrated mixture is excellent in stability. It can be said.

Although the embodiments of the present invention have been described above with reference to the accompanying drawings, those skilled in the art to which the present invention pertains may implement the present invention in other specific forms without changing the technical spirit or essential features thereof. I can understand that. Therefore, it is to be understood that the embodiments described above are exemplary in all respects and not restrictive.

1 to 4 are photographs of antimicrobial activity assay for MRSA of Gujeolcho, Buddha, Panaxia, Pasqueflower, and natural concentrated mixture using Kirby-Bauer method.

Claims (12)

Gujeolcho, Buddha Son, Eoseongcho, Hwanggyeongpi, Gosam, Dandelion, Prunus bark, Honeysuckle, Trichophyllum and Pasqueflower were added to each extract in 0.2 g / ml in water or ethanol to obtain respective extracts. Method for producing an antimicrobial composition comprising mixing each extract of the same amount. According to claim 1, To obtain each of the extracts The Gujeolcho, Buddha Son, Eoseongcho, Hwanggyeongpi, Ginseng, Dandelion, and cherry bark are separately extracted by mixing with boiling distilled water, The method of producing an antimicrobial composition comprising extracting the phosphorus flower, triticale, and pasqueflower individually with ethanol at room temperature. The method of claim 2, The gujeolcho, budsonson, eoseongcho, hwangkyeongpi, ginseng, dandelion, and cherry tree bark extracted by mixing with boiling distilled water is extracted for 2 hours. The method of claim 2, The extract of the phosphorus flower, triticale, and pasqueflower individually mixed with ethanol at room temperature to extract the extract while shaking for 7 days. According to claim 1, After mixing the same amount of each extract, Method for producing an antimicrobial composition further comprises concentrating the mixed extract. 6. The method of claim 5, Concentrating the mixed extract, The solvent of the mixed extract is evaporated to dryness, Method for producing an antimicrobial composition comprising eluting the dried mixed extract with an organic solvent. The method according to claim 6, Wherein said organic solvent is ethyl alcohol. The antimicrobial composition manufactured by the manufacturing method of any one of Claims 1-7. delete delete delete delete

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