KR101566089B1 - a cgt composition with the prevention and treatment for atopic dermatitis - Google Patents

Abstract

The present invention is directed to a technique which is effective for atopic dermatitis using herbal medicine or herbal medicine and more specifically to atopic dermatitis produced by blending herb, ginseng, ginseng, sage, guinea, incheon, ≪ / RTI >
The present invention provides a composition having efficacy for atopic dermatitis, which is a mixture of a herb extract, a ginger extract, a ginseng extract, a sorbitol extract, a prick extract, a Prunus persica extract, a Huang Baek extract and a licorice extract.
The present invention also relates to a herbicidal composition comprising 80 to 120 parts by weight of a ginseng extract, 80 to 120 parts by weight of a ginseng extract, 60 to 80 parts by weight of a ginseng extract, 60 to 80 parts by weight of a guinea root extract, 60 to 80 parts by weight of a ginseng extract, 20 to 40 parts by weight of a yellowish white extract, and 10 to 20 parts by weight of a licorice extract.
The present invention also provides a pharmaceutical composition comprising the above-mentioned composition effective for atopic dermatitis.

Description

[0001] The present invention relates to a composition for the prevention and treatment of atopic dermatitis,

The present invention is directed to a technique which is effective for atopic dermatitis using herbal medicine or herbal medicine and more specifically to atopic dermatitis produced by blending herb, ginseng, ginseng, sage, guinea, incheon, ≪ / RTI >

Atopic dermatitis (AD) is a typical refractory disease among allergic diseases, which means inflammation of the skin surface causing persistent pruritus. Usually closely related to dry skin, itching, accompanied by inflammation, asthma, allergic rhinitis, personal and family history of atopic eczema, ^{2, 3).} Most of them are caused by genetic factors, but various environmental factors such as changes in dietary habits due to urbanization and pollution, skin dryness, house dust mites, animal hair and secretions also act as inducers.

Among these, the environmental factors such as changes in environmental pollution and food intake are expected to become worse and worse, and the prevalence of AD is expected to increase even more. Actually, the prevalence of AD has been reported to increase continuously in the last few years. In addition, if adequate treatment for AD in infancy is not made, 2 out of 3 patients are reported to be converted to asthma or allergic rhinitis and appropriate treatment is required.

There are various risk factors depending on the type of dermatitis, but genetic causes are common causes in each disease. Th2 immunosensitivity in AD is the greatest risk factor and affects serum IgE levels in dermatitis patients. In immunological mechanisms, the balance between Th1 and Th2 cytokines is broken by antigens, and the over-produced Th2 cytokines IL-4, 5, 9, and 13 stimulate B cells to increase immunoglobulin E (IgE) . Increased IgE binds to the Fc e RI receptor on the mast cell surface (cross-linked), and when the allergen penetrates back into the human body, allergen and allegen-specific IgE on the Fc e RI receptor It binds and sensitizes mast cells to secrete histamine and other chemicals stored in these cells. These chemicals irritate blood vessels and skin, causing red spots, swelling and itching on the skin and causing or exacerbating AD. In conclusion, one of the greatest features of AD is that serum IgE levels are higher than normal people, and IgE is known to act as a marker for AD.

Recently, inflammatory cells such as CD4 + T lymphocytes as well as IgE have been reported to play an important role in the development of AD. T lymphocytes differentiate into Th1 and Th2 cells and Th2 cells with interleukin (IL-4, IL-5 , IL-9, and IL-13, and Th1 cells produce IL-2, IFN-, and TNF-a. Th2 cytokines play an important role in the development of atopy.

However, glucocorticoids and calcinulin inhibitors FK-506 (tacrolimus) have been widely used in ointments for the treatment of atopic dermatitis. Antihistamines, steroids, cyclosporins, and antimetabolites have been used for systemic administration , There is a demand for the development of a novel therapeutic agent having safety and excellent efficacy owing to various side effects.

As a result of the above-mentioned demands, atopic treatments using natural materials or herbs have been developed.

Patent Application No. 10-0742502 ("Atopic treatment herbal composition", hereinafter referred to as "prior art") is used to treat and obtain "herbal medicine, herbal medicine, malt, ginkgo, And atrophy containing powder obtained by fermenting the material mixed with pouring water, malt, ginkgo, ginseng, gyeongryeong, noujin, newgok, mungbean, broccoli into brown sugar, koji and pouring water for 48 hours, Therapeutic herbal composition ".

However, such prior art also has problems in that it is inferior in efficacy as an atopy treatment agent,

The present invention is intended to solve the problems of the prior art and the prior art described above.

Accordingly, the present invention provides a composition which is effective for atopic dermatitis using a natural material having little side effects.

The present invention also provides a composition of a natural material which is simple in its production method and is effective for prevention and treatment of atopic dermatitis.

In order to solve the above problems and needs,

Wherein the composition is effective for atopic dermatitis, which is a mixture of Ganoderma lucidum extract, Ganoderma lucidum extract, Ganoderma lucidum extract, Ganoderma lucidum extract, Chrysanthemum extract, Prunus persimilis extract,

The present invention also relates to a herbicidal composition comprising 80 to 120 parts by weight of a ginseng extract, 80 to 120 parts by weight of a ginseng extract, 60 to 80 parts by weight of a ginseng extract, 60 to 80 parts by weight of a guinea root extract, 60 to 80 parts by weight of a ginseng extract, 20 to 40 parts by weight of a yellowish white extract, and 10 to 20 parts by weight of a licorice extract.

The present invention also provides a pharmaceutical composition comprising the above-mentioned composition effective for atopic dermatitis.

The composition according to the present invention produces no side effects such as skin toxicity.

In addition, the composition according to the present invention exhibits an effect that toxicity is not exerted on liver and kidney even when administered.

In addition, the composition according to the present invention exhibits a remarkably high anti-inflammatory effect of atopic dermatitis.

In addition, the composition according to the present invention shows a remarkably high effect for prevention and treatment of atopic dermatitis.

1 shows the result of cytotoxicity test of the composition according to the present invention.
FIG. 2 shows the results of measuring the anti-inflammatory effect (total nitric oxide (NO) production inhibitory effect) of the composition according to the present invention.
Fig. 3 shows the results of measuring the anti-inflammatory effect (cytokine production amount) of the composition according to the present invention.
FIG. 4 shows the results of measurement of IgE in the serum of the experiment of the effect of the composition according to the present invention on the serum immunoglobulin production.
FIG. 5 shows the results of measurement of IgG1 in the serum of the experiment of the effect on the amount of immunoglobulin produced in the serum of the composition according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition having efficacy in atopic dermatitis prepared by mixing a herb extract, a ginger extract, a ginseng extract, a sour extract, a prince extract, a herb extract, a yellowish white extract, and a licorice extract.

(The composition having an effect on atopic dermatitis according to the present invention described above will be described as Chenggihaedok-tang or "CGT" in the following description of experiments and the like)

The composition of the present invention is characterized in that 80 to 120 parts by weight of Ganoderma lucidum extract, 80 to 120 parts by weight of ginseng extract, 60 to 80 parts by weight of saury extract, 60 to 80 parts by weight of guinea extract, 20 to 40 parts by weight of a yellowish white extract, and 10 to 20 parts by weight of a licorice extract.

The composition of the above extract may be either a method of extracting the raw materials together or a method of extracting and mixing them separately.

In the present invention, the Japanese chestnut is also called a tongue, and it grows in the grasses of the mountains. The roots are thick and purple, deeply penetrating into the ground. The stem is straight, the branches are split, the height is 30 ~ 70cm, and there are many hairs on the whole upward. Leaves are alternate phyllotaxis, 3 ~ 7cm long, basal or long elliptical, with pointed end, narrow bottom, petiole, and coarse hairs. The front surface of the leaf has deep wrinkles along the veins, the edges are flat, and there are no petiole.

Flowers bloom in May to June and hang on the end of stems and branches with inflorescence. Bract is leaf-shaped, calyx is green, and is deeply divided into 5 pieces. The split piece is wide-lined and has a dull end and longer than the tubular part of the corolla.

Corolla is 6 ~ 7mm in length and 5 pieces in end, and cracked pieces are round and spread horizontally. There are 5 stamens and 1 pistil. The fruit is a branch and is grayish white, smooth and shiny.

In one room, the roots are used as herbal medicine called 草 草. It is effective for bleeding, nosebleeds, hemorrhage and measles, and it is externally used as a disinfectant for burns, frostbite, eczema, rash, skin ulcers. In the past, it was used as a purple dye. It is distributed in Korea, Japan, China and Amur.

Gold silver flower is the flower bud of Lonicera japonica Thunberg or its varieties in Korea. Same in China and Japan

The name "Indong" is derived from the fact that the vine is alive and does not faint even during the cold winter. The first name of the gin-ehwa is the name of the first blooming flower, but gradually turned yellow.

This medicine has a peculiar smell, flavor is sweet and quality is cold. [甘寒]

Gwanghwasa is used when fever is reduced, when the chest is frustrating and when there is a thirst. It is good for inflammation, it produces boils, skin that has torn skin, inflammation of organs, and nausea. It is also used for dermatological necrosis caused by dysentery, digestion, mastitis. Colitis, stomach ulcers, cystitis, sore throat, tonsillitis, bronchitis, conjunctivitis and swelling, high fever due to mumps, and purulent infections.

The pharmacological actions were antimicrobial, antiinflammatory, antipyretic, increased leukocyte phagocytosis, central nervous stimulation, serum cholesterol lowering, and ulcer prevention.

Its appearance is a small rod or a funnel-shaped bud and often a lips-shaped flower. The outer surface is yellowish-white or rusty-white. The longer it is stored, the darker the color. On the magnifying glass, the light brown hairs are dense, the calyx is green, the ends are divided into 5 pieces, the split pieces have hairs, and the length is about 2 mm. There are 5 stamens, yellow stamen, 1 pistil, and no hair in the stomach.

Other names include 忍冬 花, 爱 花, 银花, 双花, 金花, 金 equal flower, gold silver), (鴛鴦 藤), Rosa (鷺 藤), Noongsu (老翁 須), the left (纏 藤), Gold Chae (金 髪 股), Tongyeongcho ).

Gosam (苦 蔘) is also known as the sperm tree of a thief's stick, pork, or snake. It grows in a sunny grass. It is green with a height of 80 ~ 100cm, but it is black when it is young. The stem is straight, leaves are alternate, odd numbered compound leaf. Small leaves are 15 ~ 40, long oval or long ovate, 2 ~ 4cm long, 7 ~ 15mm butterfly. The petiole is long and the edge is flat.

A flower stem of about 20cm comes out from June to August at the tip of a branch, and a butterfly-shaped light yellow flower blooms in a gunshot. Flower length is 15 ~ 18mm. Calyxes are like tubs, with hairy outer surface and shallow ends with 5 ends. The fruit is a bead-like shape. It is short and has a length of 7-8cm and ripens in September-October.

In one room, dried roots are called roasted roasted roasted roasted roasted roasted roasted roasted ginseng. In the private sector, it is also used as an insecticide by dying stem or leaf. They are the same plants and have mountain peaks. They look very similar. They are distributed in Korea, Japan, China, and Siberia.

It is a perennial plant of rosemary rosaceae and is grown in grasses or rice fields.

The roots fall from the node with the vine hanging sideways. Leaves are alternate, leaves on roots are leaves with 3 small leaves, small leaves are egg-shaped or egg-shaped, 2 ~ 3.5cm long, 1 ~ 3cm wide. This shape has serrations on the edges of the leaves and long hairs on the back. The stipule is egg-shaped and the edge is flat.

Flowers bloom from April to May in yellow, long flower stalks come out from the axil of leaves, and one flower runs at the end. Calyx is ovate, calyx is divided into 5 pieces and again divides into 3 shallows. The petals are wide ovate and 5 ~ 10mm long. Fruits are achene, ripened in June, round, rounded to 1cm in diameter and can be eaten. It is distributed in Korea, China, Japan, Malaysia and India.

Injin refers to a medicinal plant (Korea) that has been dried above the ground of Artemisia capillaris Thunb. The Japanese have used the headscarf of Japanese apricot. The Chinese have used A. capillaris Thunb. And Artemisia scoparia Waldst . et Kit .: 濱 蒿) is using the above ground. A. iwayomogi is used in Korea as Han-In-Jin

Hwaseongcho (魚 腥草) is the underside of the flowering period of Houghtuynia cordata Thunberg in Korea. In Japan, the same plant is called 약 薬 (十 药). In China, the same plants are used, but they are called outposts or overheads.

It is a name attached because it is fishy fish in the leaf. In ancient China's Qin country, it was called the author (菹 子), a smelly vegetable plant, which was called Sujebi, which is similar to the (()).

This medicine has a fishy smell when it rubs the leaves and rubs the leaves, and the taste is spicy and the quality is a little cold. [辛 寒]

Hwasungcho is excellent for diarrhea and drainage. It is used for coughing caused by lung abscesses, pneumonia, pneumonia, acute bronchitis, enteritis, urinary tract infections, boils, and it is used when you can not see urine.

Antimicrobial activity, immune enhancement, antiinflammatory, diuretic, and calming action have been reported by pharmacological actions.

Appearance has vertical wrinkles on the stem and the nodes are clear. The lower nodes have thin roots, and the vagina is thin and easy to break. Leaves are dried or shrunk, but they are heart-shaped when opened. Leaves are pointed with sharp ends, dark yellowish green or tan on the upper side, and gray or gray brown on the underside.

Hwangbaek is a medicinal herb that is derived from the bark of an acorn tree. It is a deciduous broad-leaved arboreous tree with a height of 10m. The shell has developed cork and the inner skin is yellow. Leaves are opposite to each other, with double leaf petiole, 5 ~ 13 petioles, egg-shaped lanceolate or long round. There are small sawtooth and soft hairs on the edge of the leaf, and there are some long hairs on the base of the middle vein. Yellow flowers bloom in May-June, which is a monotony of diatoms. The conical shape is a long circular shape, the male flower has 5 stamens, and the female flower has degenerated stamens. It has 5 chambers, style is short and stigma is divided into 5 branches. The fruit has a lot of flesh of 黄果 相 (果果 状), it has a hard core in the center, and when it is cooked in round shape, it has a black color. It is distributed in all regions except Jeju and Jeonnam regions in Korea. It is distributed in Japan, Manchuria, China, Amur, and Sochi, and grows in forests or mountain valleys.

Peel off the trunk from the trunk before and after removing the bark (rough skin) and cut and dry in the sun. This shell is used as a medicine to lower blood sugar. It also inhibits growth of pneumococcal bacteria, Mycobacterium tuberculosis, Staphylococcus aureus, etc., inhibits the growth of tumor cells, and has a sterilizing action. When taking it, the stimulation of the gustatory reflex promotes the secretion of gastric juice, and it also leads to an increase in appetite.

Licorice is licorice (Glycyrrhiza uralensis Fischer), European licorice (Glycyrrhiza glabra L.) or other root and stem parts of plants are peeled or peeled in Korea and Japan. In China, dried roots of licorice (Glycyrrhiza uralensis Fischer: licorice), licorice (Glycyrrhiza glabra L .: light 果 licorice), licorice (Glycyrrhiza inflata Batal: 果 甘草) are used.

Licorice harmonizes the toxicity of all medicines and makes the medicinal effect appear, regulates the fever and morale of the book, makes good communication of all blood vessels, and strengthens the muscles and bones.

Pharmacological action is effective in detoxification, hepatitis, urticaria, dermatitis, eczema. Jinhae, geomung, muscle relaxation, diuretic, anti-inflammatory action and suppress peptic ulcer.

The present invention is characterized in that 80 to 120 parts by weight of a ginger extract, 80 to 120 parts by weight of a ginseng extract, 60 to 80 parts by weight of a ginger extract, 60 to 80 parts by weight of a guinea extract, 60 to 80 parts by weight of a ginger extract, 25 to 40 parts by weight of a yellowish white extract, and 10 to 20 parts by weight of a licorice extract, in an amount effective for atopic dermatitis.

In the present invention, it is preferable to dry and use the above-mentioned material, but it is also possible to use it without drying.

The extract of the present invention can be obtained by immersing the aforementioned materials in water and heating them to obtain an extract.

Further, in the present invention, in order to obtain a higher concentration of the extract, the above materials are thoroughly washed with distilled water, filled with distilled water of the same volume, and extracted with hot water using a reaction electric heater.

The filtrate was filtered using 0.45 μm and 0.1 μm filter paper continuously, and the inorganic salts in the filtrate were removed by precipitation with pharmacopoeial salt, adjusted to pH 7.3 with citric acid and Na 3 PO 4, Can be used.

In addition, the present invention can obtain an extract by adding the material to alcohol (alcohol) and refluxing for 2 to 4 hours.

The present invention relates to a herbal composition comprising 80 to 120 parts by weight of a ginger extract, 80 to 120 parts by weight of a ginseng extract, 60 to 80 parts by weight of a ginseng extract, 60 to 80 parts by weight of a guinea extract, 60 to 80 parts by weight of a ginseng extract, 25 to 40 parts by weight of a yellowish white extract and 10 to 20 parts by weight of a licorice extract are mixed to provide an antioxidant composition (CGT) effective for atopic dermatitis.

The present invention also provides a pharmaceutical composition comprising an antioxidant composition (CGT) effective for atopic dermatitis.

The above pharmaceutical composition means a drug for the prevention and treatment of arthritis, which means that the herbal detoxification composition (CGT) of the present invention is included therein.

The present invention was found to be effective in the prevention and treatment of atopic dermatitis by animal experiment with the herbal detoxification composition (CGT) prepared by the above method.

Animal experiments to be performed were as follows: a mixture of 30 g of prime mallow, 30 g of ginger, 30 g of ginseng, 20 g of syrup, 20 g of persimmon, 20 g of ginseng, 10 g of yellowtail, and 10 g of licorice (CGT composition) obtained by refluxing for 3 hours through 80% alcohol (1.2 liters).

Thus, the above extract was lyophilized to 50 ml using a rotary evaporator, concentrated, and the completely dried CGT composition (20.5 g) was stored frozen and used as a sample.

1. Animal experiments

NC / Nga Mice (16 ~ 22g), male, 4 weeks of age, was supplied from Orient Bio and fully supplied with solid feed (Purina) and water until the day of the experiment and maintained at a temperature of 22 ± 2 ° C and a humidity of 55 ± 15% , And 12 hours-12 hours (light-dark cycle). This experiment was conducted in accordance with the animal ethics code of the Animal Experimental Ethics Committee of the Great War of the Antarctic (Animal Use Ethics Committee Approval Number - DJUARB 2013-028).

2. Cytotoxicity experiment

(1) Raw 264.7 cells were seeded at 2 × 10 ⁴ cells / well in a 96-well plate and cultured for 24 hours. Before the experiment, the culture medium was replaced with fresh culture medium, CGT was treated at a concentration of 1, 10, 100 (μg / ml) for 24 hours. After incubation, 10 μl of WST solution was added and reacted in a cell incubator (37 ° C, 5% CO ₂ ) for 30 minutes. After the reaction, the absorbance at 450 nm was measured and the cell survival rate of the control group was expressed as a percentage.

(2) RAW 264.7 cell survival rate was 112.1 ± 3.9%, 111.6 ± 1.3%, and 103.0 ± 1% at the concentrations of 1, 10 and 100 μg / ml, respectively, when the control group was 100.0 ± 1.0% And 4.1% cell viability (Fig. 1).

3. Anti-inflammatory efficacy measurement

(1) Nitric oxide (NO) measurement

NO concentration was measured by griess reagent system. Raw 264.7 cells were plated at 2 × 10 ⁴ cells / well in a 96-well plate and cultured for 24 hours. After cultivation, the culture medium was replaced with fresh culture medium, and CGT was treated with a concentration of 1, 10, 100 (μg / ml) and 1 μg / ml of LPS, respectively, for another 24 hours. N1 buffer (50 μl) was added to each well and reacted at room temperature for 10 min. Then, 50 μl of N2 buffer was added to each well and reacted for 10 min. After the reaction, the absorbance was measured at 540 nm. The concentration of NO in the culture medium was determined using the standard curve of concentration of nitrite standard.

The NO production of RAW 264.7 cell line was 34.7 ± 3.2% in control group, 100.0 ± 3.1% in control group, 104.9 ± 2.0% in 1 μg / ml group and 10 μg / ml concentration in CGT group 103.1 ± 1.0%, and 92.8 ± 2.6% at the concentration of 100 μg / ml, respectively, and showed a significant decrease (***: P <0.001) at the concentration of 100 μg / ml compared to the control (FIG. 2).

(2) Measurement of cytokine production amount

Luminex was used to measure inflammatory cytokines in the cells. Raw 264.7 cells were plated at a density of 2 × 10 ⁵ cells / well in a 12-well plate and cultured for 24 hours. The CGT extract was treated with a concentration of 1, 10, 100 (μg / ml) 1 μg / ml, and cultured in a cell culture incubator (37 ° C, 5% CO ₂ ) for 24 hours. After centrifugation, IL-1β, IL-6 and TNF-a were measured as supernatant.

&Lt; Influence on IL-1β production amount>

In the RAW 264.7 cell line, the amount of IL-1β produced by the CGT extract was 12.59 ± 4.47 pg / ml in the control group, 5.85 ± 0.26 pg / ml in the normal group, and 17.45 ± 4.46 (pg / ml), 14.92 ± 2.88 (pg / ml) at 10 μg / ml and 13.37 ± 1.12 (pg / ml) at 100 μg / ml concentration (FIG.

4. Effect on serum immunoglobulin production

(1) IgE levels in the serum after the end of CGT administration were 31.1 ± 0.7 (ng / ml) in the 7th week, 33.5 ± 1.1 (ng / ml) in the 9th week and 37.9 ± 0.3 (ng / ml) at 9th week, 41.1 ± 1.8 (ng / ml) at 13th week, 32.7 ± 1.0 (ng / ml) at 9th week and 226.1 ± 11.1 (ng / ml) at 9th week, 219.7 ± 7.8 (ng / ml) at 13th week, 33.4 ± 1.2 ng / ml at 7th week, 256.9 ± 3.2 (ng / ml) at 13 weeks, 31.9 ± 0.8 (ng / ml) at 7th week, 252.9 ± 10.7 (ng / ml) at 9th week, and 128.7 ± 0.5 (ng / ml) and 95.6 ± 4.2 (ng / ml) at 13th week, showing a significant decrease (***: P <0.001) compared to the control group (FIG.

(2) IgG1 was measured in the serum after the end of CGT administration. As a result, in the normal group, 2217.1 ± 176.1 μg / ml, 4913.8 ± 141.2 μg / ml in the 7th week and 2783.9 ± 98.8 μg / ml), and CGT-treated group showed 3012.4 ± 41.7 (μg / ml), showing a significant decrease (**: P <0.01) compared to the control group (FIG.

The composition of the present invention is effective for prevention and treatment of atopic dermatitis without side effects such as cytotoxicity as described above.

The present invention provides an antioxidant composition and a pharmaceutical composition containing the same, which are highly effective for preventing and treating atopic dermatitis without side effects.

The present invention is very useful in an industry for producing, processing, distributing and selling functional foods and medicines for treating atopic dermatitis using herbal medicines and herbal medicines.

Claims (3)

Ginseng extract, ginseng extract, ginseng extract, chrysanthemum extract, persica extract, mandwon extract, persimmon extract, licorice extract,
80 to 120 parts by weight of Ganoderma lucidum extract, 80 to 120 parts by weight of ginseng extract, 60 to 80 parts by weight of saury extract, 60 to 80 parts by weight of guinea extract, 60 to 80 parts by weight of Rhizoma extract, 40 parts by weight of a licorice extract and 10-20 parts by weight of a licorice extract.
delete A pharmaceutical composition comprising an effective amount of atopic dermatitis according to claim 1.

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