KR20150061839A

KR20150061839A - Manufacturing method of composition of enzyme for preventing lose of hair and Composition of enzyme for preventing lose of hair manufactured by the same

Info

Publication number: KR20150061839A
Application number: KR1020130146067A
Authority: KR
Inventors: 이경원
Original assignee: 이경원
Priority date: 2013-11-28
Filing date: 2013-11-28
Publication date: 2015-06-05

Abstract

The present invention relates to a method for producing an enzyme composition for preventing hair loss and an enzyme composition for preventing hair loss, the method comprising: aging a first mixture to prepare a first aged product (step 1); Aging the second mixture to produce a second aged material (step 2); Filtering the first aged material and the second aged material to obtain a first aged liquid and a second aged liquid (step 3); Mixing the first aging solution and the second aging solution to prepare a mixed solution (step 4); Aging the mixed solution (step 5); And culturing the aged mixed solution (step 6); And has the advantage of being useful for a cosmetic composition for preventing hair loss and promoting hair growth even without side effects.

Description

[0001] The present invention relates to a method for producing an enzyme-inhibiting composition for preventing hair loss, and an enzyme-inhibiting composition for preventing hair loss,

The present invention relates to a process for producing an enzyme composition for preventing hair loss and an enzyme composition for preventing hair loss, more particularly, to a process for preparing an enzyme composition for preventing hair loss, which is useful for preventing hair loss and promoting hair growth, And an enzyme composition for preventing hair loss.

Human hair is an important part of the body expressing personality and personality. Unlike other mammals, human hair is considered to be important in terms of aesthetic and sexual rather than biological purposes. About 50-100 of the normal hair fall off naturally, and hair loss is defined as dehydration, which is caused by excessive withdrawal of hair due to various reasons. The most common hair loss is alopecia due to immune system abnormality and male hair loss caused by age. The precise mechanisms of hair growth and hair loss are not fully understood until now. However, the cause of hair loss is the decrease of hair follicle function due to the involvement of male hormone, the decrease of the metabolism function of hair follicle and parental part, the local blood flow obstruction due to scalp tension, Stress, drug side effects, genetic factors, autoimmunity, local infections, chemicals, leukemia, tuberculosis and abuse of hair products. The most typical form of hair loss, androgen alopecia, occurs genetically androgen-hormone dependent. The typical androgen hormones of mammals include testosterone and dihydrotestosterone (DHT), which cause hair loss More patients are reported to have DHT. Here, testosterone is converted to dihydrotestosterone by 5 alpha-reductase, especially dihydrootestosterone converted from testosterone by type 2 5 alpha-reductase in two forms present in 5 alpha-lidocytase, Resulting in progressive miniaturization of the nipple and hair follicles.

In recent years, the remarkable development in molecular biology and cell biology has transformed hair loss research into a more systematic and scientific way. Among them, the development of culture methods of various cells constituting hair follicles and hair follicles is used as a useful evaluation model while providing important clues for the study of hair loss.

According to the research report, the growth of the hair progresses as the epithelial cells surrounding the dermal papilla divide and form the hair shaft, so that the dermal papilla cells regulate the division of the epithelial cells And the role of essential male hormone in the male part of the male pattern baldness is also a part of the male hairpin, the male part of the hairpin has played an important role in hair growth. On the other hand, keratinocytes, which account for about 98% of epidermal cells, have a special function of producing complex filamentous proteins called keratin, which form not only the stratum corneum of the epidermis but also the hair and nail structure proteins Therefore, many attempts have been made to promote hair growth through the proliferation of keratinocytes.

There are many kinds of hair-related products available in the market, but most of them are not enough to prevent hair loss and hair growth, or they are temporary. Therefore, they do not meet the needs of the users and there is insufficient data on efficacy and safety. However, the effects of minoxidil and propecia, which are approved by the US Food and Drug Administration (FDA), are recognized to some extent, but when they are discontinued, it is reported that serious problems such as alopecia are recurred or serious adverse effects are reported It is true. Therefore, it is urgently required to develop an effective drug that has not only been proved by an objective and reproducible evaluation technique, but also has few side effects.

Korean Patent Publication No. 10-1237635 (Feb. 26, 2013) discloses a composition for preventing hair loss and hair growth, comprising shoemaking, shag bamboo leaf, papaya leaf, .

Although the composition has an advantage of improving hair loss, there is a possibility that side effects may occur.

KR 10-1237635 B1 201.02.26.

It is an object of the present invention to provide a process for producing an enzyme composition for preventing hair loss, which is useful for preventing hair loss and promoting hair growth, without side effects, and an enzyme composition for preventing hair loss.

In order to achieve the above object, the present invention provides the following means.

The present invention relates to a process for producing a fermented milk, comprising aging a first mixture to produce a first fermentation product (step 1); Aging the second mixture to produce a second aged material (step 2); Filtering the first aged material and the second aged material to obtain a first aged liquid and a second aged liquid (step 3); Mixing the first aging solution and the second aging solution to prepare a mixed solution (step 4); Aging the mixed solution (step 5); And culturing the aged mixed solution (step 6); 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1 mixture of plantain, 1: 1: 1: 1: 1 in a weight ratio of 1: 1: 1: 1: 1: 1: The present invention also provides a method for producing an enzyme-inhibiting composition for preventing hair loss.

The first aged material is prepared by mixing the first mixture and water in a weight ratio of 1: 1 and aging at 0 ° C to 5 ° C for 720 days to 740 days. The second aged material is mixed with the second mixture and water The mixture is mixed at a weight ratio of 1: 1 and aged at 0 ° C to 5 ° C for 720 days to 740 days.

In the step 5, the mixture is aged at 0 ° C to 5 ° C for 360 days to 370 days.

In the step 6, the aged mixed solution is cultured in sawdust at a temperature of 15 to 25 DEG C for 10 to 14 days.

The present invention also provides an anti-hair-loss enzyme composition produced by the above-described method.

The enzyme composition for preventing hair loss according to the present invention is advantageous in cosmetic composition for hair loss prevention and hair growth promotion without side effects.

Hereinafter, the present invention will be described in detail.

First, a method for producing an enzyme composition for preventing hair loss according to the present invention is provided.

The method for producing an enzyme composition for preventing hair loss,

Aging the first mixture to produce a first aged material (step 1);

Aging the second mixture to produce a second aged material (step 2);

Filtering the first aged material and the second aged material to obtain a first aged liquid and a second aged liquid (step 3);

Mixing the first aging solution and the second aging solution to prepare a mixed solution (step 4);

Aging the mixed solution (step 5); And

Culturing the aged mixed solution (step 6);

.

In the step 1, the first mixture includes plantain, hwanggi, ginseng, sorghum, mackerel, lemongrass, yuukee, cucurbitaceae, rapeseed meal, persimmon, hornblende and clay.

1: 1: 1: 1: 1: 1: 1, 1: 1, 1: 2, : 1: 1: 1.

The plantain used in the present invention is a perennial herb belonging to Plantaginaceae and the young leaves are edible. It is extracted from root in one room and used as a medicine for tea. It is distributed in Korea, Japan, Sakhalin, Taiwan, China, Siberia, Eastern Himalaya, Java and Malaysia. The components of the plantlet outpost are glycosides such as aucubin, plantaginin, homoplantaginin, and tannin, ursolic acid, and phytosterol glycosides, and these sterols Β-sitosterol palmitate, stigmasterol palmitate, and the like are the main subjects of the mucilage puffing substance. Plantaginis Semen contains plantasan, plantago mucilage A (L-arabinose, D-xylose, D-glucuronic acid, D) as mucilage (about 9% -galacturonic acid), auric acid glycosides such as aucubin, genipiosidic acid and flavone glycosides, such as plantagoside, acetoside, acetoside, syringin, choline and other fatty acids.

The plantain is used as a diuretic, shinhae, gadam, dry, branch, fever, inflammation and tonic because it contains glycoside and tannin called "planaraginin". It activates liver function and is effective for dizziness and headache. Seawater also works. It is also used for the treatment of acute chronic bacterial diarrhea, nephritis, cystitis, and urethritis. It is said that it is good if it puts the life leaf on the boil softly and attaches it to the boil and the toothache stops when the raw leaf is rubbed with salt and it is bitter with the sick. In the present invention, roots of plantain root are used.

The Astragali Radix used in the present invention has been widely used as a perennial herbarium belonging to Leguminosae which is widely distributed in Asia such as Korea and Japan and Europe including Russia and Bulgaria. Diuretics, anemia, anorexia, deprivation, and tonic. In addition, Hwanggi is included in the sacred Maternal Materia Medica. There are hundreds kinds of prescriptions of Hwanggi in Korean herbal medicine, such as guarantee Yiengtang, Hwangjiaojungbang, Hwangjungjaeojang, Is a popular medicine.

The herbal pharmacological actions known so far include hypotensive action, arteriosclerosis action, hepatoprotective action, hypoglycemic action, and immunity-enhancing action. As a physiologically active ingredient of the Hwanggi period, the triterpenoid glycoside system and flavonoid system are dominant, and other saponin and polysaccharide components are contained. In the present invention, roots, leaves and stems of Hwanggi are used as whole.

The ginseng (Panax ginseng CA Meyer. Araliaceae) used in the present invention is one of the medicines traditionally used for the treatment of various diseases in Asian countries such as China, Korea, and Japan. Ginseng saponin (ginsenoside), which is a main active ingredient of ginseng, has various physiological activities such as anti-aging, anti-inflammation, antioxidant activity in the central nervous system and cardiovascular and immune system, antidiabetic activity and antitumor activity . In the present invention, roots of ginseng are used.

The sycamore tree used in the present invention is a deciduous shrub with beans which grows frequently at a height of 2 to 3 m in the mountains and fields and blooms in July-August, and has a unique pharmacological effect and is widely used for medicinal purposes. It is known that the sycamore contains flavonoid, festidine, protein, fat, calcium, vitamin B1, amino acid, saccharide, iron, vitamin C, vitamin K and tannin chlorophyll.

In addition to excellent effect on headache, hypertension, lack of oocyte, lethargy, athlete's foot, eczema, skin disease, eye disease, cough and edema due to waste heat, it is also excellent for back pain, bruise and osteoporosis. In addition, it exerts a whitening effect by suppressing the synthesis of a signal transduction substance related to melanin synthesis, which is a substance that releases waste in the human body and causes spots, freckles and pigmentation of skin, and exerts an effect of skin softening and clarification. In the present invention, the sycamore tree uses roots.

The starfish used in the present invention is a perennial herbaceous plant of the dicotyledonous plant axilla. The leaves are alternate phyllotaxis and have a triangular ovate shape. When young, the central part is reddish with wavy sawtooth on the edge. Flowers are positive, yellowish green, no petal, calyx is divided into 5, 5 stamens and 1 pistil. The fruit is a fungus wrapped with calyx and contains black seeds. Vitamin A, B, and C are amino acids, fatty acids, linoleic acid, etc., such as leucine, betaine, and trigonelline. It also contains a small amount of essential minerals such as chromium, potassium and zinc. In particular, chromium prevents arteriosclerosis, keeps blood lipid levels normal, and metabolizes sugars with insulin. In the case of zinc, it also helps to form insulin in pancreatic cells. In the present invention, the leaves, stalks and fruits of the Japanese apricot are used as whole.

As used in the present invention, it is an annual plant of branches, and it is a native plant of many parts of Korea, usually in fields, roads and grasslands. It grows around the roads and fields of the lowlands and is 20 ~ 90㎝ high. The branches spread widely and the ridgelines appear slightly on the main stem. The herbal medicine is called yonggye (dried outpost), the outpost and fruit have been used as medicines to treat boils and malignant swelling in the civilization since ancient times. The Dongdeok Dictionary published in North Korea and the old literature in Korea and China said, "Taste, poison, heat, and circulate well. It makes urine come out well, eliminates inflammation and inhibits tumor And it is used for chemotherapy. " In addition, the outbreak of lambs has the action of killing typhoid bacteria, dysentery bacteria, Escherichia coli, red germ and pseudomonas aeruginosa, and also has a function of lowering blood sugar. In the present invention, leaf, stem, and fruit of lobster are used as whole.

The rhizome of the present invention is a bark of the elm (Ulmus davidiana var) root which has been traditionally widely used and has been widely used in the form of a health food. As for its components, it has been known to contain flavonoids, saponins, tannins, mucilages (Resin), β-sitosterol, phytosterol, sigmasterol, tannin, And Fathyoil. It is known that the medicinal efficacy and application of the medicinal products such as fungi, species, individual improvement, coughing, convergence, anti-inflammation, antiparasitic, antibacterial, diuretic, It has been known that folk remedies have been effective in repairing boils and swellings, and it has been known to exert excellent effects on various ulcers including gastric ulcers and duodenal ulcers in one or the other.

The Cudrania tricuspidata (Carr.) Bureau ex Lavallee used in the present invention is a bivalve tree belonging to the genus Chrysanthemum bisporus. , Rou- tin, and flavonoids, and is known to be effective for hypertension, diabetes, cholesterol improvement, improvement of bowel activity, antioxidant activity, and anti-cancer. The root bark, tree vine, bark, and leaves of the cedarwood tree contain a variety of ingredients effective for the human body, and thus the cedarwood tree from its origin can be used as an antihypertensive agent for tuberculosis, tuberculosis, stem, leaf, bark, It is used as medicines such as medicines, antipyretics, dry cough, expectorants, diuretic agents, hemostatic agents, and gustatory agents, and is used for athlete's foot as an antifungal agent and is used for chronic indigestion due to weakness of digestive organs. In the present invention, the roots of trees are used.

The Setaria viridis BEAUV. Used in the present invention is an annual herbaceous plant of the alomonaceae plant. It is also called a dog's tail, and it is called a umbrella. They grow on the roads. The stem is 20 ~ 70㎝ long, with branches, no hairs, and the nodes are somewhat longer. In one room, the outpost is taken in the summer and the dried one is used as medicine. It is distributed nationwide. In the present invention, the roots of the grasshopper are used.

The Wa-song (Orostachys japonicus A. Berger) used in the present invention is a perennial food of Crassulaceae that grows on old tiled roofs or rocks in deep mountains. The leaves are fleshy, It grows in a bunch. Wassong grows on the tile roof and resembles pine leaves and flowers.

Recently, phytochemicals such as sterols, triterpenoids, flavonoids and phenolics have been isolated from the liver and have been widely used for the treatment of hepatitis, It has been known that cancer of the system is effective, and researches on it have been actively conducted. In the present invention, the leaves and stems of the wafers are used as whole.

The Humulus japonicas Sieboid & Zucc. Used in the present invention is a plant belonging to the genus Neeta. It is also called a ginseng vine. The male flower is dioecious, and the male flower has 5 calyxes and stamens. . The stem that grows on the vines has a hook-shaped backgauge facing downward and sticks to other objects. Leaves on opposite sides are palmate and split into 5-7 pieces with regular sawtooth on the edge and rough hairs on both sides. The fruit is oak and ripens to yellowish brown.

The hornblende has the effect of releasing fever, urinating well, eliminating eosinophilia, and releasing poison from the body. It also treats urinary discomfort, schizophrenia, diarrhea, dysentery, pulmonary tuberculosis, lung abscess, and pneumonia. In the present invention, the roots of the hornblende are used.

The terra alba used in the present invention is also called white pine, white pine and kaolin, and is white clay used for making pottery. The main ingredient is composed of kaolinite and halloysite.

The major components are SiO 60.85 ~ 67.63%, AlO 15.64 ~ 21.68%, FeO 1.65 ~ 3.84%, MgO 2.93 ~ 3.94%, CaO 0.20 ~ 1.11%, NaO 2.94 ~ 3.2% and ignition loss 3% The most fundamental effect is the far-infrared rays that are emitted from clay.

In particular, white clay removes toxins from the human body and is also used as an antidote and antidote, and the beneficial infrared rays are radiated and absorbed by the human body, thereby facilitating metabolism, thereby preventing aging, promoting metabolism, preventing chronic fatigue, And it is known that it has an effect of preventing red tide phenomenon.

Preferably, the first aged material is aged at 0 ° C to 5 ° C for 720 days to 740 days after mixing the first mixture and water at a weight ratio of 1: 1. If the aged material is aged at less than 0 ° C, , There is a risk of decomposition if aged at a temperature higher than 5 ° C.

It is preferable to use the water which is snow-melted, the water which is lower in the pollution degree than the general water and contains a lot of nutrients, and the snow melted in the winter is used.

In the step 2, the second mixture includes an omija, a spiraea, a persimmon, a lobster, a dog peach and clay.

Preferably, the second mixture is prepared by mixing 1: 1: 1: 1: 1: 1 by weight of Omija, Daria, Persimmon,

The Schizandra chinensis used in the present invention is a deciduous plant of the genus Schizandra (Schizandra chinensis). The fruit is mostly circular, ripe in August to September, 1 to 2 seeds, strong in sour taste, contains medicinal ingredient, It contains ingredients such as Schizandrin, Gomisin, Citral, Malic acid and Citric acid which strengthens the heart and lowers the blood pressure and strengthens the immune system. It is known that it has the effect of treating cough and thirst by strengthening the pulmonary function, and having the action of chinhwa and geodam.

Such an omija has been used as a food such as tea, green tea, ceramics, and liquor, and has been used as a medicinal medicine of oriental medicine since ancient times. The above-mentioned Omija refers to the fruit of Schizandra chinensis.

Actinidia arguta used in the present invention belongs to Actinidiaceae and originates in Siberia, northern China, and Korea. More than 30 different species have been reported, including A. colomikta, A. polygama, A. rupa, and generally known as kiwi, A. chinensis or A. deliciosa) are also belonging to the family Acanthaceae. It is used as a medicinal herb named Mifudo. It contains fruits of fruit trees and related plants, has a high quality and is not toxic, and has been prescribed for the treatment of liver disease and gastrointestinal diseases or urological stone treatment. The above-mentioned variety means the fruit of the blueberry tree.

The ginseng used in the present invention is one of nutrients having high nutritional value which has been used in various forms in civil use since the past. It contains a large amount of calcium and is effective for diuretic action. The content of vitamin C is richer than oranges and apples It has excellent effect to increase the resistance to the disease and the immune system. It also has a high content of vitamin A, which makes the skin strong and elastic. For this reason, it has been reported that hypertension, arteriosclerosis, resolution of hangover, prevention of cold, anti-aging and anti-cancer effect. In the present invention, a native feeling is used.

In the present invention, a tortoise is a fruit of a rapeseed, which is much smaller and harder than a normal pear, and is thick and thin.

The peaches used in the present invention refers to wild peaches inhabited in a natural state, which is also called a stone peach. Dog peaches and ordinary peaches can be said to be the same, but dog peaches are wild in nature, whereas ordinary peaches are improved by humans and cultivated by using chemical fertilizers. , And there is a significant difference in the ingredients contained.

The peach is a representative medicinal substance found in main medical books such as the main herbarium and Dongbokgam, and it treats the clogged blood because it does not have blood circulation and blood circulation, and eliminates bad aura. It also helps constipation, diarrhea, edema, and vengeance. It is effective to cool the skin, freckles, spots, menstrual irregularities, menstrual coughs, coughs, arthritis, athlete's foot, eczema, facial nerve paralysis Cure. In the present invention, peach fruit is used.

Preferably, the second aged material is aged at 0 ° C to 5 ° C for 720 days to 740 days after mixing the second mixture and water in a weight ratio of 1: 1. If the aged material is aged at less than 0 ° C, , There is a risk of decomposition if aged at a temperature higher than 5 ° C.

It is preferable to use water in which the snow is melted.

Step 3 is a step of filtering the first aged material to remove solids to obtain a first aged liquid, filtering the second aged material to remove solids, and obtaining a second aged liquid.

Step 4 is a step of mixing the first aging solution and the second aging solution in a weight ratio of 1: 1 to prepare a mixed solution.

In the step 5, it is preferable that the mixture is aged at 0 ° C to 5 ° C for 360 days to 370 days. When it is aged at 0 ° C or less, it is not fermented, and if it is aged at 5 ° C or more, it is corroded.

In the step 6, the aged mixed solution is preferably cultured in sawdust at a temperature of 15 to 25 ° C for 10 to 14 days. If the agar mixed liquid is cultured at a temperature of less than 15 ° C, , There is a possibility that the enzyme is rapidly grown and weakened by the cold.

In addition, the present invention provides an enzyme composition for preventing hair loss, which is produced by the above-mentioned production method.

The total content of the inventive anti-hair loss enzyme composition contained in the hair loss preventing cosmetic composition is preferably 0.001 to 10% by weight based on the total weight of the cosmetic composition. If the content of the enzyme preparation for preventing hair loss is less than 0.001% by weight, it can not sufficiently achieve the intended hair loss prevention. If the content is more than 10% by weight, the stability of the cosmetic may be problematic , There is a disadvantage that sufficient cosmetic effect can not be exhibited when the appearance and use of the cosmetic material are used.

The cosmetic composition for preventing hair loss is not limited to the moisturizing agent, the thickener, the surfactant, the oil base agent, the preservative, the antioxidant, the alcohol, the fragrance, the pH adjuster and the natural extract, The scalp and hair enhancement component of the composition can be formulated in suitable amounts.

Meanwhile, the cosmetic composition prepared by containing the enzyme composition for preventing hair loss of the present invention can be manufactured in various formulations such as cosmetic lotion, essence, skin, lotion, cream, shampoo, Phase, pasty, and the like, and can be produced using a conventional cosmetic preparation method.

Claims

Aging the first mixture to produce a first aged material (step 1);
Aging the second mixture to produce a second aged material (step 2);
Filtering the first aged material and the second aged material to obtain a first aged liquid and a second aged liquid (step 3);
Mixing the first aging solution and the second aging solution to prepare a mixed solution (step 4);
Aging the mixed solution (step 5); And
Culturing the aged mixed solution (step 6);
, &Lt; / RTI &
The first mixture contains 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1 ratio of plantain, hanagi, ginseng, sorghum, 1: 1: 1: 1,
Wherein the second mixture is prepared by mixing 1: 1: 1: 1: 1: 1 weight ratio of Omija, Darya, persimmon, peper, dog peach and clay.

The method according to claim 1,
The first aged material is prepared by mixing the first mixture and water in a weight ratio of 1: 1 and aging at 0 ° C to 5 ° C for 720 days to 740 days,
The second aged material is prepared by mixing the second mixture and water in a weight ratio of 1: 1 and aging at 0 ° C to 5 ° C for 720 days to 740 days,
Wherein the water comprises water dissolved in an eye.

The method of claim 1, wherein step (5)
And aging the mixed solution at 0 ° C to 5 ° C for 360 days to 370 days.

The method of claim 1, wherein step (6)
Wherein the aged mixed solution is cultured in sawdust at a temperature of 15 to 25 DEG C for 10 to 14 days.

An enzyme inhibitor for hair loss prevention, which is produced by the production method of any one of claims 1 to 4.