KR102092254B1 - Cosmetic Compositions for Anti-aging Comprising Extract of Setaria viridis - Google Patents

Abstract

The present invention relates to an antiaging cosmetic composition comprising a Setaria viridis extract as an active ingredient. Since the extract provided from the present invention has excellent DPPH scavenging activity, collagen generation effect and collagenase inhibition effect, the extract can be used as a cosmetic composition for antiaging and wrinkle alleviation.

Description

Cosmetic Compositions for Anti-aging Comprising Extract of Setaria viridis}

The present invention relates to an anti-aging cosmetic composition comprising an extract of ragweed ( Setaria viridis ) as an active ingredient.

Active oxygen introduced from outside the body or generated in the body may promote aging of the body or cause cancer. Therefore, research is being conducted on antioxidants that inhibit oxidation by free radicals, and antioxidants are known as phenolic compounds, flavonoids, tocopherols, vitamin C, and selenium. However, when it is applied to the skin, antioxidants existing in nature cannot be expected to have a substantially sufficient effect. On the other hand, synthetic antioxidants, which have excellent antioxidant power and are inexpensive, have limitations in their use due to safety concerns such as human side effects.

The causes of wrinkle formation can be largely divided into the generation of wrinkles by natural aging and the generation of wrinkles by UV exposure (photoaging). The mechanism of wrinkle formation by two causes is not yet known. Common phenomena found in connection with skin aging due to natural aging and photoaging are reduced and modified collagen and elastin, the major constituent proteins in the dermis, and reduced elasticity of the skin due to reduced hyaluronic acid, the matrix of the dermis. In particular, according to research results so far, it is known that the reduction and modification of collagen and elastin, the main proteins in the dermis, are directly involved in the formation of wrinkles and the decrease in elasticity of the skin. Collagen is a major matrix protein produced by the fibroblasts of the skin. It is present in the extracellular epilepsy, and its important functions are mechanical firmness of the skin, resistance to connective tissue and tissue, support for cell adhesion, cell division and differentiation (organism Induction of growth or wound healing) is known. It is known that this collagen decreases with age and photoaging by ultraviolet irradiation, which is closely related to the formation of wrinkles on the skin. In addition, in recent years, the results of extensive research on skin aging have been reported, revealing the important function of collagen in the skin. Active ingredients are known that promote collagen synthesis and show wrinkle improvement effects. For example, retinoic acid, transforming growth factor (TGF), protein derived from animal placenta, betulinic acid, chlorella extract, and the like are known as collagen synthesis accelerators.

On the other hand, dogweed ( Setaria viridis ) is a perennial herbaceous plant of the rice plant, a monocotyledonous plant. It is also called dogtail, and it is called Gumicho in Chinese characters. It grows on roadsides or fields. The stems are 20 ~ 70cm in size, branched, without hairs, and the nodes are somewhat long. The length of the leaves is 5 ~ 20cm, the width is 5 ~ 20mm, and the bottom part becomes the leaf sheath, and there are hairs protruding from the leaves and strings at the edges. The flowers bloom in mid-July-August in mid-summer, and the circumferential spikes are 2-5 cm long and are light green or purple. The small branch spreads 6 ~ 8mm long and looks like a thorn. Seeds were used as old-fashioned plants, and in the private sector, roots were cut in September and used for tapeworms. In the oriental medicine, outposts are collected in the summer and dried are used for medicinal purposes. It is distributed nationwide. There are about 100 species in the tropics, and in Korea, similar species are the long, dense hairs of the twigs, and the grains are not clear and grow on the beach. Scourge is a hybrid of the cochlea. The purple dogweed is purple with the hair hanging on the ear, and is not discriminated and treated as a dogweed. Geumgang puppy, wrinkled puppy, autumn puppy, and fine leaf puppy are growing.

As a cosmetic composition technology using ragweed extract, in the case of Korea, Korean Patent Registration No. 10-1913235, "Method of manufacturing a cosmetic composition containing a mixed extract of baby champandi, ragweed, sky hawk claw and crimson fern", Republic of Korea Publication No. 10-2015-0061839 discloses a method of preparing an enzyme composition for preventing hair loss and an enzyme composition for preventing hair loss produced thereby.

However, there has not been a study to develop a cosmetic composition having anti-oxidant and anti-wrinkle effects using ragweed extract.

Thus, the present inventors found that the extract of ragweed ( Setaria viridis ) exhibits excellent DPPH scavenging activity, collagen production and collagenase inhibitory effect, and can simultaneously impart antioxidant and anti-wrinkle effects.

Republic of Korea Registered Patent Publication No. 10-1913235 Republic of Korea Patent Publication No. 10-2015-0061839

An object of the present invention is to provide an anti-aging cosmetic composition derived from natural products.

Another object of the present invention is to provide a method for producing an anti-aging cosmetic composition derived from natural products.

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition comprising an extract of ragweed ( Setaria viridis ) as an active ingredient.

In the present invention, the extract is characterized in that it is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin and mixed solvents thereof.

In the present invention, the extract is characterized in that contained 0.001 to 20% by weight relative to the total weight of the composition.

In the present invention, the extract is characterized by having DPPH scavenging activity, collagen production and collagenase inhibitory efficacy.

In addition, the present invention provides a cosmetic comprising the composition.

Since the extract provided from the present invention has excellent DPPH scavenging activity, collagen production, and collagenase inhibitory efficacy, it can be used as a cosmetic composition for antioxidant and wrinkle improvement.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art.

Throughout the present specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

According to an embodiment of the present invention, the present invention relates to an anti-aging cosmetic composition comprising an extract of ragweed ( Setaria viridis ) as an active ingredient.

The extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention belongs, and specifically, from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, propylene glycol, glycerin and mixed solvents thereof. It may be extracted with a selected solvent.

Specifically, the content of the extraction solvent may be 2 to 100 times the weight of the plant solids, more preferably 5 to 50 times, and most preferably 10 to 20 times.

In addition, the extract can be extracted at room temperature or by heating under conditions in which the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and ultrasonic extraction, cold extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction, etc. may be used depending on the type and extraction method.

Filtration is a process of removing suspended solid particles from the extract, but it is possible to filter out the particles using cotton, nylon, paper, etc., or use an ultrafiltration method, a freezing filter method, or a centrifugation method.

The step of concentrating and drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of grinding the final dried extract may be added.

According to an embodiment of the present invention, the extract may be processed and used in various forms, such as a fermentation broth, a powder obtained by drying the extract, and a concentrate obtained by concentrating the extract.

According to an embodiment of the present invention, the ragweed ( Setaria viridis ) extract obtained from the above method may be included in an amount of 0.001 to 20% by weight based on the total weight of the composition, and more preferably 0.1 to 1% by weight. If the extract is less than 0.001% by weight relative to the total weight of the composition, the function of the composition is poor because the effect of improving wrinkles, anti-oxidation, and wrinkles cannot be sufficiently obtained. Not only is it uneconomical because it can exhibit efficacy, it is also undesirable because it is difficult to use as a cosmetic product due to the reduced stability and spreadability of the formulation.

The extract is characterized by having DPPH scavenging activity, collagen production and collagenase inhibitory efficacy. That is, since it is possible to simultaneously impart antioxidant and anti-wrinkle effects, it has an effect of not only simply skin beauty, but also fundamental skin health improvement.

As described above, since the extract has excellent antioxidant and wrinkle improvement efficacy, it can be used as a composition for antioxidant and wrinkle improvement.

According to an embodiment of the present invention, when used as the cosmetic composition, external ointment for skin, cream, softening lotion, convergent makeup, nutrient makeup, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, spray , Gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, It may have any one formulation selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, titanium oxide, etc. may be used as a carrier component. You can.

When the formulation of the present invention is a body gel, in addition to the above-mentioned active ingredients, carbomer, triethanolamine, menthol, ethanol, glycerin, disodium ID, A, methylparaben, fragrance, etc. This can be used. Here, carbomer, triethanolamine acts as a thickener, menthol acts as a fragrance component, ethanol functions to impart a refreshing feeling, glycerin acts as a moisturizer, and disodium IDEA chelates metal components It functions as, and methylparaben is added for its function as a preservative. In addition, various scents such as herb scent may be added to the scent in addition to the menthol flavor.

In addition to the specific components described above, the production of a composition by applying well-known materials widely used in the art to achieve the functions of the thickener, refreshing feeling, fragrance component, and moisturizer described above is also within the scope of the present invention. Can be included.

When the formulation of the present invention is a powder or a spray (spraying agent), lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be added as a carrier component, especially in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is added as a carrier component, examples of which are water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan and the like.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide ether as carrier components Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

According to one embodiment of the present invention, the anti-aging cosmetic composition may be prepared with a composition such as body gel, body lotion, body cream, body oil for promoting fat decomposition by adding a conventional additive, and also manufactured as an aerosol type Can be. In this case, the cosmetic composition is preferably used as a method of transdermal administration to be applied or sprayed directly on the skin.

According to one embodiment of the present invention, the amount of the anti-aging cosmetic composition can be appropriately adjusted according to individual differences, formulations, and forms, such as age and skin fat condition, and is useful as a composition for antioxidant and anti-wrinkle effects. Can be used.

The composition obtained from the above method can provide excellent anti-oxidation and anti-wrinkle cosmetics by exhibiting DPPH scavenging activity, collagen production, and collagenase inhibitory efficacy.

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to explain the present invention in more detail, and according to the gist of the present invention, the scope of the present invention is not limited by these examples to those skilled in the art to which the present invention pertains. It will be self-evident.

<Example 1> Preparation of ragweed extract

1L of 75% (v / v) ethanol aqueous solution was added to 100 g of ragweed (gathering), followed by warm extraction at 60 ° C for 3 hours. After extraction, it was filtered with filter paper, the extraction process was repeated twice, and then concentrated under reduced pressure to prepare a final powdered ragweed extract.

<Example 2> Preparation of ragweed extract

To 10 g of hedgehog extract obtained in Example 1, 400 mL of 75% (v / v) ethanol aqueous solution was added to dissolve again, and pectin hydrolysis enzyme (Pectinex) from Novozyme, Denmark was added, followed by shaking culture at 40-70 ° C. Did. After incubation and concentration under reduced pressure, a final pectin hydrolase treated ragweed extract was prepared.

<Example 3> Preparation of ragweed extract

100 mL of distilled water was added to 10 g of pectin hydrolase-treated extract of ragweed obtained in Example 2, and then 100 mL of ethyl acetate was added, followed by fraction concentration to prepare an EA fraction of the final pectin hydrolase-treated ragweed extract. Did.

<Experimental Example 1> Antioxidation test: DPPH scavenging activity

Free radical scavenging activity was evaluated in order to investigate the antioxidant effect using the extracts of ragweeds of Examples 1 to 3.

The free radical scavenging activity experiment is a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299 ~ 303, 1993), a stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl) , Sigma) reagent was used. First, 1 mL of 0.2 mM DPPH solution was diluted with methanol to the appropriate concentration in Examples 1 to 3, mixed with 2 mL, reacted for 10 minutes at room temperature, and absorbed at 517 nm with a microplate reader (Synergy, BioTek, USA). Was measured. On the other hand, the control in the free radical scavenging test was measured in the same way by adding methanol instead of the sample solution, and methanol was substituted for the DPPH solution to obtain color correction values for each. The experiment was performed three times each, and expressed as an average value ± standard deviation, and the free radical elimination rate was calculated using Equation 1 below.

[Equation 1]

Free radical scavenging rate (%) = 100- (absorbance of each extract sample / control absorbance) x 100

division Erase rate (%) 0.25 mg / mL 0.50 mg / mL 1.00 mg / mL Example 1 17.75 ± 2.15 31.61 ± 3.81 58.28 ± 0.56 Example 2 25.32 ± 1.83 48.56 ± 2.67 72.56 ± 6.14 Example 3 28.22 ± 3.28 52.68 ± 5.41 85.21 ± 7.05

As a result, as can be seen in Table 1, all of Examples 1 to 3 exhibited antioxidant effects, and among them, it was confirmed that Examples 3 showed better antioxidant effects.

<Experimental Example 2> Cytotoxicity test: MTT assay

Cytotoxicity experiments were performed using fibroblasts (HS68 cells) to confirm that they had toxicity to the cells using the extract of ragweeds of Examples 1 to 3.

Human-derived fibroblasts (HS68 cells) were distributed from the American Type Culture Collection (ATCC), and contained in 10% FBS (fetal bovine serum, Gibco BRL, USA) DMEM medium (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) It was incubated under 37 ℃, 5% CO ₂ using. The cultured HS68 cells were divided into 96-well plates at 1x10 ⁴ cells / mL per well, and then cultured for 24 hours. After cultivation, the ragweed extracts of Examples 1 to 3 were replaced with DMEM medium without serum, diluted by concentration, and cultured for 24 hours. After 24 hours, after adding CCK-8 (Cell Counting Kit, Dojindo, Japan) solution of 1/10 of the cell culture solution, and incubating at 37 ° C. for 2 hours, absorbance at 450 nm was measured using a Microplate reader. . The experiment was performed three times each, and expressed as an average value ± standard deviation, and was calculated using Equation 2 below.

[Equation 2]

Cell viability (%) = 100- (absorbance of each extract sample / control absorbance) x 100

division Cell viability (%) 0 μg / mL 10 μg / mL 20μg / mL 50μg / mL 100 μg / mL Example 1 100 106.03 ± 3.98 108.44 ± 5.15 117.22 ± 1.41 76.09 ± 4.20 Example 2 100 103.86 ± 1.81 106.75 ± 4.99 106.48 ± 1.75 108.70 ± 2.17 Example 3 100 115.98 ± 4.29 127.04 ± 2.87 130.30 ± 6.03 74.38 ± 10.98

As a result, as can be seen in Table 2, Example 2 did not show cytotoxicity to a concentration of 100 μg / mL, and Examples 1 and 3 showed cytotoxicity at a concentration of 100 μg / mL or more. Therefore, Example 2 was carried out with 100 μg / mL and Examples 1 to 3 with 50 μg / mL concentration as the highest concentration.

<Experimental Example 3> Wrinkle improvement efficacy test: Measurement of collagen production effect

Collagen production effect using fibroblasts was measured in order to investigate the wrinkle improvement effect using the extracts of ragweeds of Examples 1 to 3.

First, cultured fibroblasts (HS68 cells) cells were dispensed at 1x10 ⁵ cells / mL per well into a 24-well plate, and then cultured for 24 hours. After cultivation, the ragweed extracts of Examples 1 to 3 were replaced with DMEM medium without serum, diluted by concentration, and cultured for 24 hours. The amount of collagen synthesis was measured using a collagen measurement kit (Procollagen Type IC peptide EIA kit, Takara, Japan) to measure the amount of procollagen type I C-peptide (PICP). The absorbance of the non-sampled cell culture solution was taken as 100% of the control, and the experiment was performed three times each to represent the mean value ± standard deviation.

division PIP (%) 0 μg / mL 10 μg / mL 20μg / mL 50μg / mL 100 μg / mL Example 1 100 123.3 ± 6.0 127.3 ± 8.7 131.0 ± 8.5 - Example 2 100 113.8 ± 5.3 117.0 ± 4.0 110.1 ± 3.4 112.4 ± 5.9 Example 3 100 113.1 ± 4.6 137.9 ± 8.4 142.2 ± 5.7 -

As a result, as can be seen in Table 3, all of Examples 1 to 3 were excellent in the collagen production effect, and among them, in Example 3, the collagen production effect was excellent, confirming that the wrinkle improvement effect was remarkably high.

<Experiment 4> wrinkle improvement efficacy test: collagenase (MMP-1) inhibition measurement

The collagenase (MMP-1) inhibitory efficacy using fibroblasts was evaluated in order to investigate the wrinkle improvement effect using the extracts of ragweeds of Examples 1 to 3.

First, the cultured fibroblasts (HS68 cells) cells were dispensed at 1x10 ⁵ cells / mL per well into a 24-well plate, and then cultured for 24 hours. After incubation, UV A was irradiated to induce the generation of collagenase, and the ragweed extracts of Examples 1 to 3 were replaced with DMEM medium without serum, diluted by concentration, and cultured for 48 hours. For the collagenase inhibitory effect, the amount of collagenase was measured using a collagenase measurement kit (MMP-1, Human, Biotrak ELISA System, GE Healthcare, USA). The absorbance of the non-sampled cell culture solution was taken as the control 100%, and the experiment was performed three times each to represent the mean value ± standard deviation.

division MMP-1 (%) 0 μg / mL 10 μg / mL 20 μg / mL 50 μg / mL Example 1 100 97.7 ± 0.2 97.2 ± 2.9 81.5 ± 0.5 Example 2 100 120.4 ± 2.4 101.3 ± 11.6 93.6 ± 9.1 Example 3 100 83.6 ± 4.6 54.3 ± 0.4 19.1 ± 1.3

As a result, as shown in Table 4, it was confirmed that in Example 3, an excellent collagenase inhibitory effect was exhibited and the wrinkle improvement effect was remarkably high.

Since the specific parts of the present invention have been described in detail above, it will be apparent to those of ordinary skill in the art that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

(A) treating the ethanol extract of hedgehog with pectin hydrolase; And
(B) obtained by a method comprising the step of fractionating the pectin hydrolase treatment solution with ethyl acetate
Antioxidant and anti-wrinkle cosmetic composition containing ragweed ( Setaria viridis ) extract as an active ingredient.


delete The method of claim 1, wherein the extract is foxtail (Setaria viridis) Antioxidant and anti-wrinkle cosmetic composition containing the extract as an active ingredient, characterized in that comprises 0.001 to 20% by weight relative to the total weight of the composition.
According to claim 1, The extract is DPPH scavenging activity, collagenase and collagenase inhibitory efficacy, characterized in that it has a dogweed ( Setaria viridis ) extract, as an active ingredient, antioxidant and anti-wrinkle cosmetic composition.
Cosmetics comprising the composition of any one of claims 1, 3 and 4.

(A) treating the ethanol extract of hedgehog with pectin hydrolase; And
(b) A method for producing an extract of prickly pear ( Setaria viridis ) having antioxidant and anti-wrinkle effects, comprising the step of fractionating the pectin hydrolase treatment solution into ethyl acetate.