KR101757469B1 - Compositions for promoting hair growth and inhibiting inflammation comprising mixed extracts of natural products - Google Patents

Abstract

The present invention relates to a hair growth stimulating and antiinflammatory composition comprising a natural product complex extract, and more particularly to a composition containing an alfalfa extract and a colasby extract, and a composition comprising a broccoli extract, And at least one extract selected from the group consisting of at least one extract selected from the group consisting of the following.
The composition of the present invention has an effect of activating a factor promoting hair growth in the skin and increasing the expression, thereby creating a skin environment for promoting hair growth and suppressing skin irritation. And can be useful for developing functional foods and cosmetics that promote hair growth.

Description

Technical Field [0001] The present invention relates to a hair growth promoting and anti-inflammatory composition containing a natural product complex extract,

The present invention relates to a hair growth stimulating and antiinflammatory composition comprising a natural substance complex extract, and more particularly to a composition containing an alfalfa extract and a cola extract, a broccoli extract, a cisterna tulobulus extract and a sea tangle extract Gt; hair growth promoting and anti-inflammatory food or cosmetic composition which additionally comprises at least one extract selected from the group consisting < RTI ID = 0.0 > of: < / RTI >

Hair is a protein filament that grows in the hair follicle of the skin dermis. The hair follicles are composed of a dermal papilla, a matrix surrounding the dermal papilla, and a root sheath. The dermis is mainly composed of connective tissue and capillary blood vessels. It surrounds the hair follicles and supplies nutrients and oxygen. The matrix is composed of epithelial cells and melanocytes, and cell division is active. Here the keratinization of the cleaved cells creates hair and grows out of the skin. The rootstock has inner root sheath and outer root sheath, which protects the growing hair until it is completely cured and reaches the epidermis of the skin. The hair grows periodically through the growing / anagen-catagen-telogen-elimination phase (natural hair loss), repeating the process of growing and falling back. Hair growth begins in the hair follicle, where hair cells grow in the bulge of the external appearance and form a new substrate during the early growth period, and the hair shaft and hair shaft from relatively undifferentiated basal cells A root is formed. The size and length of the hair stem are proportional to the size of the hair follicle and the duration of the hair growth period, respectively.

Alopecia is a condition in which hair is normally abnormally low in density or disappears, and is largely divided into scarring and non-scarring. Cicatric alopecia is a major cause of trauma, burns, tumors, surgery and other scars and infections, as hair follicles are destroyed and restored to fibrous tissue, resulting in permanent hair loss. Non-scarring alopecia is a condition in which the tissue is not fibrous and the hair follicles are preserved, such as alopecia areata, resting / growing alopecia, alopecia areata, male hereditary androgens alopecia. The cause of hair loss is not clear, but the cause of environmental and genetic factors such as air pollution, dietary habits, stress, hormone imbalance, autonomic nervous system and blood circulation disorder, excessive sebum production, hair follicle malnutrition, allergy, It is presumed that physiological factors such as aging are combined. Alopecia has occurred only in middle - aged people in the past, but recently it has occurred frequently in young people in their 20s and 30s.

In order to improve the hair loss phenomenon and to promote hair growth, research and development of cosmetics or pharmaceuticals have been actively carried out. Currently, the drugs of the United States Food and Drug Administration (FDA) are licensed for the treatment of hair loss. Minoxidil, There is finasteride. Minoxidil dilates blood vessels to increase blood flow and nutrients to hair follicles to induce hair growth. Pinasteride is a specific inhibitor of type II 5α-reductase that prevents testosterone from being converted to dihydrotestosterone, It is known to inhibit. These hair growth medicines are effective only in the area where the hair follicle is activated, so they are effective in preventing hair loss. However, there is a drawback in that the growth effect of the hair which is in the resting state for a long time is insignificant or the effect is maintained only during the medication. In particular, drugs that regulate male hormones can cause side effects such as loss of libido and impotence.

Therefore, it is necessary to develop a hair growth promoting technology which can prevent hair loss, promote hair growth, maintain its effect stably, and have less side effects and is safe and economical.

Accordingly, the inventors of the present invention have investigated natural materials that can promote hair growth and inhibit skin irritation that may cause hair loss. Among them, alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Kelp extract of the present invention has excellent hair growth promoting effect and anti-inflammatory effect, thus completing the present invention.

It is therefore an object of the present invention

And a composition for promoting hair growth and containing anti-inflammation, which comprises alfalfa extract and collagen extract.

Another object of the present invention is

The present invention also provides a cosmetic composition for promoting hair growth and anti-inflammation comprising alfalfa extract and collagen extract.

In order to achieve the above object,

A composition for promoting hair growth and for anti-inflammation, which comprises alfalfa extract and columbia extract, is provided.

In order to achieve another object of the present invention,

The present invention provides a cosmetic composition for promoting hair growth and anti-inflammation comprising alfalfa extract and collagen extract.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for accelerating hair growth and anti-inflammatory food comprising alfalfa extract and columbia extract.

The composition for food of the present invention comprises alfalfa extract and cola extract, and in order to further enhance hair growth and anti-inflammatory effect, one or more extracts selected from the group consisting of broccoli extract, It may be additionally included. The alfalfa extract, cola extract, and broccoli extract according to the present invention may be preferably extracted from each bud.

The inventors of the present invention found that a mixed composition of alfalfa sprout extract and cola non-sprout extract can be used as an anti-inflammatory agent in skin, such as gamma-glutamyl transpeptidase, alkaline phosphatase (ALP), epidermal growth factor (EGF) And enhances the activity and / or expression level of factors that induce hair growth such as growth factor (VEGF), thereby creating a skin environment that promotes hair growth, and has an excellent effect in inhibiting skin inflammation that may cause hair loss and dandruff . Particularly, when the above-mentioned mixed composition further contains a broccoli sprout extract, a cystatin sputum roots extract, and a seaweed extract, hair growth promotion and anti-inflammatory effects are further enhanced, and the present invention is disclosed for the first time. The present invention can be applied to the development of safe and effective hair growth promoting and anti-inflammatory food by using the effect of the plant-algae mixed extract composition according to the present invention.

The hair growth promoting and anti-inflammatory effects of the plant-algae mixed extract composition confirmed by the present inventors are as follows.

In one embodiment of the present invention, in the skin of a mouse to which a mixed composition of an alfalfa bud extract and a colasvir sprout extract has been administered, gamma-glutamyl transpeptidase (gamma GTP), which is known to induce anagen, ). The activity of increasing the γGTP activity was further increased when the alfalfa sprout and the cola sprout extract were further administered with the broccoli sprout extract, the cystatin sprout extract and the sea tangle extract. γGTP is an enzyme that transduces a gamma glutamyl functional group and exists in the hair follicles of the hair follicle and the hair follicle, and the activity is greatly increased in the hair growth period (Kawabe TT et al., J Invest Dermatol , 103 (1): 122-6, 1994).

In another embodiment of the present invention, the activity of alkaline phosphatase (ALP) is remarkably increased in the skin of mice administered with the mixed composition of alfalfa sprout extract and cola sprout extract, and the activity of broccoli sprout extract, It was confirmed that ALP activity was further elevated when Bullosa extract and Sea tangle extract were added. ALP is an enzyme that hydrolyzes phosphate and is present in the mesenchymal and epithelial cells of the hair follicle, and the amount and activity of hair growth in the dermal papilla, especially in the dermal papilla, is greatly increased (Iida M et al., Dev Growth Differ , 49 (3): 185-95, 2007).

In another embodiment of the present invention, the expression amount of epidermal growth factor (EGF), which promotes cell proliferation and differentiation and promotes hair growth in the skin of a mouse administered with a mixed composition of alfalfa sprout extract and collagen sprout extract, , And the amount of EGF expression was significantly increased when Broccoli sprout extract, Sisoteca tubulosa extract, and Sea tangle extract were further administered. EGF is expressed at the early stage of anagen, inducing the hair follicle to grow into the growth phase, promoting the growth of the hair shaft, decreasing the amount of expression in the catagen, (KK et al., J Biol . 1987). In addition, it has been shown that hair regrowth of the hair regrowth regulates the hair growth cycle, Chem. , 278 (28): 26120-6, 2003).

In another embodiment of the present invention, the expression level of vascular endothelial growth factor (VEGF), which promotes neovascularization in the skin of mice administered with the mixed composition of alfalfa sprout extract and collagen sprout extract, In addition, the addition of broccoli sprout extract, cis - tootrobula extract and sea tangle extract increased the VEGF expression level more remarkably. VEGF promotes hair growth and blood circulation in hair follicles, promoting hair growth and increasing hair follicle and hair size (Yano K et al., J Clin Invest , 107 (4): 409-417, 2001).

In another embodiment of the present invention, a mixture of alfalfa sprout extract, colbai sprout extract and broccoli sprout extract has a synergistic effect to inhibit nitric monoxide (NO), an inflammatory mediator produced in macrophage RAW 264.7 cells. Respectively. In particular, the combined extract composition containing all of the five extracts of plants and seaweeds has the effect of remarkably lowering the NO production amount.

In addition, the body (in the present invention in other embodiments of the mixed composition containing the alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cis tanche-to-fire use extracts and / or seaweed extract, etc. vivo anti-inflammatory effects were confirmed by mouse experiments. The amount of exudate produced by skin inflammation induced by subcutaneous air infusion and carageenan, which is an inflammation inducer, in a mouse administered with a mixed composition of alfalfa sprout extract, cola sprout extract or additionally a broccoli sprout extract, , And concentrations of NO and prostaglandin E2 (prostaglandin E2, PGE ₂ ), which are inflammatory mediators in the exudate, were significantly lower. This effect was even better when it included all five plants and seaweed extracts.

The results of the above examples show that the mixture of the alfalfa sprout extract and the cola sprout extract exhibits excellent control of the hair growth factor and anti-inflammatory effect. In particular, in addition to the alfalfa sprout extract and the colubi sprout extract, the broccoli sprout extract, And / or kelp extract. ≪ / RTI > Thus, a combination of alfalfa extract and cola extract, or a combination extract of Broccoli extract, Sisotanthus bulurosa extract and / or sea tangle extract, may be used to promote hair growth and cause hair loss and dandruff It is possible to develop a functional food having an excellent inhibitory effect on inflammation.

Refers to hair, hair, facial hair and / or hair, including hair, eyelashes, eyebrows, mustache, beard, ear hair, nose hair, chest hair, conspiracy, secondary hair, etc. However, It does not.

The term " hair growth promotion " as used herein refers to promoting hair growth, facilitating structural stabilization of hair follicles and facilitating nutrient and oxygen supply, faster induction of growth period of new hair cycle, prolongation of growth period of hair cycle, Or an increase in hair density, which includes, but is not limited to, inducing hair growth and making it more visible to the eye.

The 'anti-inflammatory' in the present invention inhibits the molecular cellular inflammatory reaction such as the invasion of immune cells, the proinflammatory cytokine, the secretion of proinflammatory factors such as prostaglandin and nitric oxide (NO), and the increase of erythema, , Congestion, fever, pain, etc. to prevent, mitigate or improve. Excessive inflammation in the skin can cause hair loss and dandruff.

In the present invention, 'alfalfa ( Medicago sativa ) is a perennial plant of soybean which is also called " lucidum " in Korea and widely cultivated as edible, medicinal and mainly livestock feed crops. The alfalfa in the present invention may be a bud of alfalfa or alfalfa.

In the present invention, kohlrabi (kohlrabi), the des (Brassica oleracea with Brassica genus gongil oleracea spp Gongylodes), also known as german turnip or turnip cabbage. Cobra leaves and pale green or purple bulbs are used for food. In the present invention, the cola bean may be a cola bean or a cola bean sprout.

In the present invention, 'broccoli' is a plant belonging to Brassica oleracea , also called green flower cabbage. One of the cabbages to eat the flower part began to be improved in Italy and became breeded to suppress the development of the flower, resulting in a present appearance with numerous floret from a thick stalk. Both stem and flower buds are edible. The broccoli in the present invention may be broccoli or broccoli sprouts.

In the present invention, the term " Cistanche " tubulosa 'is a parasitic plant of the desert belonging to the genus Cistanche , also called mandarin breeding . It does not have chlorophyll and grows by absorbing water and nutrients from host plants. Before the germination, the stem of the cystanthus tubulosus was different from the other plant in the cistern body, Cistanche salsa , Cistanche < RTI ID = 0.0 > deserticola , and Cistanche ambigua , and have been used as medicinal herbs for infertility, recreation and the like.

In the present invention, 'kelp ( Saccharina japonica ) is a perennial giant algae distributed in the Pacific coast. According to the old classification method, Laminaria japonica , Laminaria ochotensis and so on. Fucoidan, a polysaccharide, and inorganic salts such as iodine are abundant.

The extracts of alfalfa, cola, broccoli, cysteine, and sea tangle can be prepared by selecting appropriate extraction solvents and extracting them by acid / base extraction, hot water extraction, room temperature stirring extraction, cold extraction, Pressure extraction, hot-pressure extraction, low-temperature high-pressure extraction, enzyme-treatment extraction, solvent extraction, and the like. Examples of the extraction solvent include alcohols having 1 to 6 carbon atoms such as water, methanol, ethanol, alcohol, propanol, isopropanol, butanol, pentanol and butoxyethanol, ethylene glycol, acetone, ether, diethyl ether, known conventional solvents such as petroleum ether, chloroform, ethyl acetate, methylene chloride, n-hexane, cyclohexane, acetone, benzene, or a mixed solvent thereof. Can be extracted.

Preferably, the extract of the present invention may be extracted with any extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, alcohol, and a mixed solvent thereof, and most preferably, water or an aqueous ethanol solution is used as an extraction solvent can do.

In addition, the extract of the present invention may be an extract firstly extracted by an extraction method known in the art, or may be re-extracted after first extraction and then mixed with first extract to enhance extraction efficiency. In addition, the extract of the present invention can be purified and purified by chromatographic separation, diatomic filtration, ultrafiltration or the like in accordance with methods known in the art in order to remove impurities and increase the concentration of the active ingredient . The final extract can be concentrated using known concentration methods such as precipitation concentration, evaporation concentration, azeotropic concentration, concentration under reduced pressure, distillation concentration, centrifugation, reverse osmosis, etc., and concentrator, and can be concentrated by a method such as freeze drying, spray drying, To remove the solvent and to solidify it into a powder form.

The extract of the present invention can be obtained by, for example, extracting water or an aqueous solution of ethanol as an extraction solvent, removing the impurities by filtering the extract, removing the extraction solvent using a vacuum evaporator, Followed by hot-air drying to obtain an extract powder. The specific method of producing the extract used in the examples of the present invention is as described in the experimental method of the examples.

The food composition of the present invention preferably contains alfalfa extract and columbia extract at a weight ratio of 1: 0.1 to 10 for promoting hair growth and anti-inflammatory effect.

In addition, in order to further promote hair growth and anti-inflammatory effects, the food composition of the present invention may further contain one or more extracts selected from the group consisting of broccoli extract, sistant extract, and sea tangle extract in addition to alfalfa extract and cola extract . When the composition comprises the alfalfa, collavi, broccoli, cisterna tubulosa, and sea tangle extract, the combined extract composition contains the extract of each individual alone at a ratio of 1: 0.1-10: 0.1-5: 0.1-30: 0.1-10 It is preferable that it is included in a weight ratio. In addition, even when one or two kinds of extracts of Broccoli extract, Sistant body tobacco extract and Sea tangle extract are additionally contained, the weight ratio of each extract can be applied as it is in the weight ratio of the above-mentioned complex extract composition (for example, If the composition of the present invention contains alfalfa, cola, and broccoli extract, the weight ratio of each may include 1: 0.1 to 10: 0.1 to 5).

As described above, the alfalfa extract, the collavi extract and the broccoli extract may be preferably extracted from each bud.

The composition for food using the complex extract composition according to the present invention includes all forms such as functional food, nutritional supplement, health food and food additives. These types can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, the food composition itself of the present invention may be prepared in the form of tea, juice, and drink and then consumed, granulated, encapsulated, and powdered. In addition, the food composition of the present invention can be prepared in the form of a composition by mixing together with a known substance or active ingredient known to have an effect of promoting hair growth and anti-inflammation.

Functional foods also include beverages (including alcoholic beverages), fruits and their processed foods (such as canned fruits, bottled, jam, maalmalade, etc.), fish, meats and processed foods such as ham, (Such as udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, , Vegetable protein, retort food, frozen food, various kinds of seasoning (for example, soybean paste, soy sauce, sauce, etc.).

The preferred content of the food composition according to the present invention is not particularly limited, but is preferably 0.01 to 50% by weight based on the total weight of the final food product. In order to use the food composition of the present invention in the form of a food additive, it may be used in the form of powder or concentrate.

The present invention Alfalfa  Extract and Cobrabi  For promoting hair growth including extracts and for anti-inflammation Cosmetics  Lt; / RTI >

It is preferable that the alfalfa extract and the cola extract are contained in the cosmetic composition at a weight ratio of 1: 0.1 to 10 for promoting hair growth and anti-inflammatory effect.

In addition, the cosmetic composition according to the present invention may further comprise one or more extracts selected from the group consisting of broccoli extract, cystathanthin flourosa extract and kelp extract to further enhance hair growth promotion and anti-inflammatory effects, in addition to alfalfa and cola extract . When all three of the above extracts are included, the alfalfa extract, the cola sprout extract, the broccoli extract, the sicutan tuloblusa extract and the sea tangle extract are mixed at a ratio of 1: 0.1-10: 0.1-5: 0.1-30: 0.1-10 It is preferable that it is contained in cosmetics in a weight ratio. In addition, even when one or two kinds of extracts of Broccoli extract, Sistant body tobacco extract and Sea tangle extract are additionally contained, the weight ratio of each extract can be applied as it is in the weight ratio of the above-mentioned complex extract composition (for example, If the composition of the present invention contains alfalfa, cola, and broccoli extract, the weight ratio of each may include 1: 0.1 to 10: 0.1 to 5).

As described above, the alfalfa extract, the collavi extract and the broccoli extract may be preferably extracted from each bud.

The method of extracting the above extract or the extraction solvent is as described in the food composition of the present invention.

The cosmetic composition using the extract composition according to the present invention can be prepared into any formulation conventionally produced in the art. In addition to the above extract, in addition to the dermatologically acceptable medium, base, adjuvant, excipient and the like, (Foundation, lipstick, mascara, make-up base), composition for scalp or hair (such as shampoo, hair, Rinse, hair conditioner, hair gel, hair cream, hair pack, hair mask, hair essence) and soap. The cosmetic composition according to the present invention can be preferably prepared as a composition for hair or scalp such as eyebrows, eyelashes, hair, and the like.

The excipient includes, but is not limited to, an emollient, a skin permeation enhancer, a colorant, a fragrance, an emulsifier, a softener, a thickener, a thickener, a gelling agent, a preservative, a stabilizer, a foaming agent, Active agents, fillers, and solvents. In addition, it may further include perfumes, coloring matters, bactericides, antioxidants, preservatives, adjuvants, and moisturizers, and may include thickeners, inorganic salts, and synthetic high molecular substances for the purpose of improving physical properties. For example, when the cleanser and the soap are prepared using the cosmetic composition of the present invention, the complex extract composition according to the present invention can be easily added to a common cleanser and soap base. In the case of producing a cream, it can be prepared by adding the extract of the present invention to a cream base of a typical underwater type (O / W). A synthetic or natural material such as a flavor, a chelating agent, a coloring matter, an antioxidant, an antiseptic, and a protein, a mineral, and a vitamin for the purpose of improving a physical property may be further added.

Formulations of suitable cosmetic compositions include, for example, solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in water, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) In the form of a foliar dispersant, in the form of a cream, a skin, a lotion, a powder, an ointment, a spray or a conceal stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The content of the cosmetic composition according to the present invention is not limited thereto, but it is preferably 0.001 to 50% by weight based on the total weight of the final cosmetic composition. When the content of the extract is less than 0.001% by weight based on the weight of the total cosmetics, it is not preferable since the desired hair growth promotion and anti-inflammatory effect can not be sufficiently attained. If the content exceeds 50% by weight, And it is not preferable because of the disadvantage that the appearance of the cosmetic or the cosmetic effect can not be sufficiently exhibited when the cosmetic is used.

Accordingly, the present invention relates to a composition for promoting hair growth and anti-inflammation and a composition for cosmetic composition, which further comprises at least one extract selected from the group consisting of alfalfa extract, cola extract, broccoli extract, cis-tootrobula extract and sea tangle extract . The composition of the present invention has an effect of increasing the activity and / or expression of a factor promoting hair growth in the skin, thereby creating a skin environment for promoting hair growth and an excellent effect of suppressing skin inflammation that can cause hair loss and dandruff .

FIG. 1 shows the activity of γ-glutamyl transpeptidase measured on mouse skin treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract, seaweed extract alone or mixture for 14 days. * Indicates a statistically significant difference ( p < 0.05) compared to the control (Normal).
FIG. 2 shows the activity of alkaline phosphatase measured on mouse skin treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract, seaweed extract alone or mixture for 14 days. * Indicates a statistically significant difference ( p < 0.05) compared to the control (Normal).
FIG. 3 shows the expression levels of EGF measured on alfalfa bud extract, cola sprout extract, broccoli sprout extract, cystatin tetuburosa extract, kelp extract alone or in mixture with mouse skin for 14 days. * Indicates a statistically significant difference ( p < 0.05) compared to the control (Normal).
FIG. 4 shows the expression levels of VEGF expressed in alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract, kelp extract alone or in mixture with mouse skin for 14 days. * Indicates a statistically significant difference ( p < 0.05) compared to the control (Normal).
Figure 5 shows the concentrations of NO measured in RAW 264.7 cell culture medium treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract, seaweed extract alone or mixture thereof and stimulated with IFN gamma and LPS. * Indicates a statistically significant difference ( p < 0.05) compared to the positive control (IFN-y + LPS) compared to the control.
Fig. 6 shows the amount of exudate produced by inflammation induced in mouse skin treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract, seaweed extract alone or mixture thereof. * Indicates a statistically significant difference ( p < 0.05) compared to the positive control (carageenan) when compared to the normal group.
FIG. 7 shows the concentration of NO measured in exudate produced by inflammation induced in mouse skin treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, cystatin sputum extract and seaweed extract alone or in combination . * Indicates a statistically significant difference ( p < 0.05) compared to the positive control (carageenan) when compared to the normal group.
Fig. 8 is a graph showing the effect of PGE ₂ on exudate produced by inflammation induced in mouse skin treated with alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Respectively. * Indicates a statistically significant difference ( p < 0.05) compared to the positive control (carageenan) when compared to the normal group.

Hereinafter, the present invention will be described in detail.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

<Experimental Method>

Extract preparation

In this example, alfalfa, cola and broccoli were used for each bud. The extracts of alfalfa sprouts and cola sprouts were prepared by solvent extraction with 70% ethanol aqueous solution, sterilization, concentration and drying. Broccoli sprout extracts were prepared by washing broccoli sprouts, drying, pulverizing, solvent extraction with 70% ethanol aqueous solution at 40 ℃, sterilization, concentration and drying. The extract of Sisotanthus tuberosum was extracted with 80% ethanol aqueous solution, sterilized and dried to prepare powder. The kelp extract was washed, dried and pulverized, subjected to hot water extraction at 60 ° C for one day, filtered through a 0.45 μm membrane, sterilized, and concentrated and spray dried to prepare a powder.

Hair Growth Animal Experiment

Five week old male C57BL / 6 mice were received from Orient Bio (Gapyeong, Korea) and used for about 1 week after purification process. Animals were housed in cages (220 x 200 x 145 mm) for four mice. The environment of the animal laboratory was controlled at a temperature of 23 ± 2 ° C, a relative humidity of 55 ± 10%, a ventilation frequency of 12 times / hour, a lighting cycle of 12 hours, and an illumination of 150-300 Lux. Purina Rat Chow, a pellet type solid feed for laboratory animals, was supplied and received from Biopia (Gunpo, Korea), and sterilized purified water was allowed to drink freely. A dorsal part of a 6-week-old mouse (n = 8 / group) was epilated with an electric razor to induce telogen hair roots to anagen. That is, when pink hair was observed until the epilating skin was uniformly pinkish, the remaining hair was lightly shaved so as not to injure the skin with a razor.

The shampooed mice were orally administrated with each of the extracts prepared individually or as a mixture daily for 14 days, and the synergistic effect between the test material compositions was evaluated. As a positive control, minoxidil (MXD) was applied to the epilation area at a dose of about 0.2 mL every day for 14 days by using a brush from the day after epilation. The doses of the single extracts were as follows (unit: mg / mouse body weight in extracts): alfalfa sprout extract (54 mg / kg); Cobra sprout extract (54 mg / kg); Broccoli sprout extract (27 mg / kg); (54 mg / kg as extract 1; 162 mg / kg as extract 2); Sea tangle extract (18 mg / kg as extract 1; 54 mg / kg as extract 2). The doses of the mixed extracts were as follows: Mixture of alfalfa and cola sprout extract composition (mouse dose of 54 mg / kg respectively); Alfalfa sprout, cola sprout and broccoli sprout extract composition (54 mg / kg of alfalfa and colla sprout extract and 27 mg / kg of broccoli sprout extract); (Composition 1 containing alfalfa and colla sprout extracts of 18 mg / kg, broccoli sprout extracts of 9 mg / kg, sheath tincture extract, and sea tangle extract), as well as alfalfa sprout, colla sprout, broccoli sprout, 54 mg / kg of alfalfa and collabi sprout extract, 27 mg / kg of broccoli sprout extract, 162 mg / kg of cystathanthus bulurosa extract and 54 mg / kg of seaweed extract as composition 2, kg).

γ- Glutamyl transpeptidase (? GTP ) And alkaline 포스화제 ( ALP ) Activity measurement

The skin tissue extracted from the mouse was immediately frozen in liquid nitrogen, and then homogenized with 4 times of phosphate buffer (pH 7.4). The homogenate was centrifuged at 3,000 rpm for 20 minutes, and the supernatant was used for γ-GTP and ALP activity measurements. γ-GTP and ALP activities were measured by enzyme rate assay (GSCC) using p-nitrophenolphosphate (PNPP) -DEAV.

Epidermal growth factor ( EGF ) And vascular endothelial growth factor ( VEGF ) Expression level measurement

EGF and VEGF were measured by enzyme-linked immunosorbent assay (ELISA) kits (R & D Systems, Minneapolis, USA). That is, the production of two growth factors was quantitated by sandwich enzyme immunoassay using polyclonal antibodies specific for mouse EGF and VEGF according to the manufacturer's guide.

Cell culture and NO  dose

RAW 264.7 cells (1 × 10 ⁶ cells / mL) were treated with each extract and the composite composition for 5 minutes and then treated with interferon-γ (IFN-γ, 10 U / mL) and lipopolysaccharide (LPS, 10 μg / mL) was added and cultured for 24 hours. Nitrite (NO ² ), an oxidizing agent of NO, was measured as an indicator of NO. The culture was mixed with the same amount of Griess reagent (1% sulfanilamide, 0.1% naphthylethylenediamide in 2.5% phosphoric acid) and reacted at room temperature for 10 minutes. NO content was measured at 540 nm and quantified using sodium nitrite (NaNO ₂ ) standard quantitative curve.

Anti-inflammatory animal experiment

Seven week old male ICR mice were received from Korea BioLink (Korea, Voice) and used for about 1 week after purification process. Animals were housed in cages (220 x 200 x 145 mm) for four mice. The environment of the animal laboratory was adjusted to 23 ± 2 ℃, 55 ± 10% of relative humidity, 12 times / hour of ventilation, 12 hours of illumination period, and illumination of 150-300 \ Lux. Purina Rat Chow, a pellet type solid feed for laboratory animals, was supplied and received from Biopia (Gunpo, Korea), and sterilized purified water was taken freely for drinking water.

Alfalfa sprout extract, broccoli sprout extract, cystathanthin bulb extract, seaweed extract alone, or mixtures thereof were orally administered to mice (n = 8 / group) daily for 7 days. Administered alone or in combination, the dose being the same as in the hair growth animal experiment. And to evaluate the maximum synergistic effect of the extracts on the test substances. On the day of administration of the test substance, 10 mL of sterile air was injected subcutaneously under the back of the mouse to form an air-pouch. After 2 and 5 days, 5 mL of air was further infused to keep the bladder stable and 1 mL of carrageenan (1% in saline; Sigma) or only solvent was injected into the bladder Respectively.

Exudate analysis

After 6 hours of Carrageenan injection, the bladder was rinsed with 1 mL cold saline and the net volume of the lavage fluid was recorded. The inflammatory mediators NO and PGE ₂ were measured by enzyme immunoassay (EIA) using Griess reagent (Sigma) and Correlate-EIA kit (Assay Designs, Ann Arbor, Ann Arbor, USA).

Statistical processing

All measurements were expressed as mean ± standard error. When the significance was observed after one-way ANOVA analysis, significance was determined at the level of p <0.05 through Turkey's test.

< Example  1>

Effect of Combined Extract on Hair Growth

Example  &Lt; 1-1 > Glutamyl transpeptidase  Activity measurement

Glutamyl transpeptidase (γ-GTP) activity of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, systannium tubuloosa extract and seaweed extract was investigated. γ-GTP is an important enzyme for hair growth known to induce hair follicles into anagen in hair follicles. According to the experimental conditions, each extract was orally administered to mice orally for 14 days, and γ-GTP activity in mouse skin was measured.

As shown in Fig. 1 , when the alfalfa sprout extract, the cola sprout extract, the broccoli sprout extract, the cystatin sputum extract and the sea tangle extract were separately treated, the γGTP activity tended to increase as compared with the control (normal) . On the other hand, when alfalfa sprout extract and cola sprout extract were administered together (alfalfa + cola bean) and alfalfa sprout extract, cola sprout extract and broccoli sprout extract (alfalfa + cola + broccoli) In comparison, γGTP activity was statistically significantly increased. When the combined extracts (Composite Compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotec body tubulosa extract, and sea tangle extract were administered, each single extract, alfalfa sprout extract, The extracts of non - sprout and broccoli sprout showed the highest increase of γGTP activity. Especially, compared to Minoxidil (3%) which is a conventional hair growth agent, it showed similar level.

The above experimental results show that γGTP activity is increased in a mixed composition of alfalfa sprout extract and cola sprout extract. Especially, when the alfalfa and colla sprout extracts further include broccoli sprout extract, sistant body tulobos extract and kelp extract It can be seen that the γGTP activity is maximized due to the synergistic effect due to the mixing of the extract.

Example  <1-2> Akaline 포스화제  Activity measurement

The effects of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisoteca tubulosa extract and sea tangle extract alone or in mixtures on the activity of akaline phosphatase (ALP) were investigated. ALP is known to be an animal's skeletal growth and angiogenesis index. ALP activity was measured in mouse skin administered orally for 14 days, either alone or in combination, according to the experimental conditions.

As shown in FIG. 2 , when ALPHA sprout extract, cola sprout extract, broccoli sprout extract, STITCHUTE TOBULOSA extract, and sea tangle extract were individually treated, the ALP activity tended to increase somewhat in comparison with the control group. On the other hand, ALP activity was statistically significantly increased in alfalfa + cola bean extract plus broccoli sprout extract (alfalfa + cola bean) and cola sprout extract (alfalfa + cola + broccoli) Respectively. When the combined extracts (Composite Compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotec body tubulosa extract, and sea tangle extract were administered, each single extract, alfalfa sprout extract, The highest ALP activity was observed in the mixed composition of non-bud extract and broccoli sprout extract, and the increase was similar to that of minoxidil (3%), a conventional hair growth agent.

These results indicate that ALP activity is increased in a mixed composition of alfalfa sprout extract and cola sprout extract. In particular, when the alfalfa and colla sprout extracts further include broccoli sprout extract, sistant body sprout extract and seaweed extract It can be seen that the ALP activity is maximized due to the synergistic effect of mixing the extracts.

Example  <1-3> Epidermal growth factor  Measurement of expression level

We examined the effects of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, seaweed extract alone or in mixtures on the expression level of epidermal growth factor (EGF). EGF is known to promote hair growth. According to the experimental conditions, each extract was orally administered to mice orally for 14 days, and the amount of EGF expression in mouse skin was measured.

As shown in FIG. 3 , when the alfalfa sprout extract, the cola sprout extract, the broccoli sprout extract, the cystatin sputum extract, and the sea tangle extract were individually treated, the amount of EGF expression was slightly increased compared with the control (normal) Respectively. On the other hand, when alfalfa sprout extract and cola sprout extract were administered together (alfalfa + cola bean) and alfalfa sprout extract, cola sprout extract and broccoli sprout extract (alfalfa + cola + broccoli) And the amount of EGF expression was statistically significantly increased. When the combined extracts (Composite Compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotec body tubulosa extract, and sea tangle extract were administered, each single extract, alfalfa sprout extract, The extracts of non-bud and broccoli sprouts showed the most remarkable effect of increasing EGF expression. Especially, compared with the conventional hair growth agent, minoxidil (3%), similar level was observed.

The above experimental results show that the effect of increasing the expression amount of EGF in a mixed composition of alfalfa and colla sprout extracts, especially the extracts of broccoli sprout, cystatin tulobarosa and kelp extract in alfalfa and collabi sprout extract The increase in the expression level of EGF is maximized due to the synergistic effect due to the mixing of the extracts.

Example  <1-4> Vascular endothelial growth factor  Measurement of expression level

The effects of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, systannicum tubulosa extract and kelp extract alone or in mixtures on the expression level of vascular endothelial growth factor (VEGF) were investigated. VEGF is an angiogenic factor. Depending on the experimental conditions, each extract was orally administered to mice orally for 14 days, and the amount of VEGF expression in mouse skin was measured.

As shown in FIG. 4 , when the alfalfa sprout extract, the cola sprout extract, the broccoli sprout extract, the cystatin sputum extract and the sea tangle extract were separately treated, the amount of VEGF expression significantly changed compared to the control (normal) I did not. On the other hand, when alfalfa sprout extract and cola sprout extract were administered together (alfalfa + cola bean) and alfalfa sprout extract, cola sprout extract and broccoli sprout extract (alfalfa + cola + broccoli) The VEGF expression level was statistically significantly increased. When the combined extracts (Composite Compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotec body tubulosa extract, and sea tangle extract were administered, each single extract, alfalfa sprout extract, VEGF expression was more remarkable than that of non-bud extract and broccoli sprout extract. Especially, compared with the conventional hair growth agent, minoxidil (3%), similar level was observed.

The above experimental results show that the effect of increasing the expression amount of VEGF in the mixed composition of alfalfa sprout extract and cola sprout extract is enhanced, and particularly, the extract of broccoli sprout, sistant body tulobosa extract and sea tangle extract is further added to the alfalfa and colla sprout extract The increase in the amount of VEGF expression is maximized due to the synergistic effect due to the mixing of the extracts.

< Example  2>

Effect of Combined Extract on Inflammatory Response

Example  <2-1> IFN γ and LPS Macrophage-induced NO  Production amount measurement

To investigate the effect of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, systannium tubulo extract and kelp extract alone or mixture on inflammatory reaction, the production of NO in macrophages induced by IFN-γ and LPS . Each extract was treated with RAW 264.7 macrophage cells for 5 minutes, stimulated with IFN-y (10 U / ml) and LPS (10 ug / ml), and the amount of NO produced was measured .

As shown in FIG. 5 , when the alfalfa sprout extract, the cola sprout extract, the broccoli sprout extract, the sistant body tulobos extract and the sea tangle extract were separately treated, NO production was lower than that of the control (IFN-y + LPS) . On the other hand, the addition of alfalfa sprout extract, cola bean extract (alfalfa + cola bean) or broccoli extract (alfalfa + cola + broccoli) resulted in further reduction of NO production compared to the positive control. On the other hand, when the combined extracts (compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, sistant body tulobos extract and sea tangle extract were treated, NO reduction effect was the largest.

The above results show that the effect of inhibiting NO production can be remarkably increased by mixing the single extract.

Example  <2-2> Measurement of exudation of skin inflammation

To investigate the effects of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, and sea tangle extract, respectively, on the inflammatory reaction, Were measured. Depending on the experimental conditions, each extract was orally administered to mice orally for 7 days. In order to induce inflammation, sterile air was injected into the skin such as mice on the beginning of the experiment and on the 2nd and 5th days to form an air bag, and carageenan (1%, 1 ml) was injected into the air bag on the last day. And the volume of the effluent produced was measured.

As shown in FIG. 6 , when alfalfa sprout extract, colbai sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, and sea tangle extract were separately administered, the effect of significantly lowering the effusion compared to the control (Carageenan) Respectively. The addition of alfalfa sprout extract and cola bean extract (alfalfa + cola bean) or the addition of broccoli extract (alfalfa + cola bean + broccoli) further reduced exudate production. On the other hand, when the combined extracts (compositions 1 and 2) including alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotec tufurosa extract, and sea tangle extract were treated, Especially, the effusion of the mice treated with the combined extracts was found to be similar or lower than that of the normal group.

The above experimental results show that the synergistic effect of suppression of exudate formation due to inflammation in the body can be obtained by mixing the above-mentioned single extracts.

Example  &Lt; 2-3 > NO  Concentration measurement

In order to investigate the effect of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, and sea tangle extract on inflammatory reaction, the amount of NO produced by inflammation was determined by inducing inflammation in mice . The concentrations of NO in the exudates of the bladder of the mice administered with each extract alone or mixed according to the experimental conditions obtained in Example <2-2> were measured, and the experimental group and the control group were compared.

As can be seen from Fig. 7 , when alfalfa sprout extract, colbai sprout extract, broccoli sprout extract, cisterna tubulosa extract, and sea tangle extract were individually administered, the effect of significantly lowering NO production compared to the control (Carageenan) Respectively. The addition of alfalfa sprout extract and cola bean extract (alfalfa + cola bean) or broccoli extract (alfalfa + cola + broccoli) further reduced NO concentration in the effluent. On the other hand, when the composite composition (compositions 1 and 2) containing both alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisotanchus tubulosa extract and sea tangle extract was treated, the NO concentration in the effusion was lowest.

Therefore, it can be seen that a synergistic effect of inhibiting NO production in the effluent as well as suppression of exudate formation due to inflammation in the body can be obtained by mixing the extract alone.

Example  &Lt; 2-4 > PGE ₂  Concentration measurement

To investigate the effect of alfalfa sprout extract, cola sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, and sea tangle extract on inflammatory responses, inflammation was induced in mice and the amount of PGE ₂ , another inflammatory mediator, was determined . The concentration of PGE ₂ present in the exudate of the bladder of the mice administered with the respective extracts alone or mixed according to the experimental conditions obtained in Example <2-2> was measured, and the experimental group and the control group were compared .

As can be seen from FIG. 8 , when alfalfa sprout extract, colbai sprout extract, broccoli sprout extract, Sisoteca tubulosa extract, and sea tangle extract were individually administered, the effect of significantly lowering PGE ₂ production as compared with the control (Carageenan) . The alfalfa sprout extract and alfalfa + cola bean extracts (alfalfa + cola bean) or broccoli extract (alfalfa + cola + broccoli) also reduced PGE ₂ concentration in the effluent. On the other hand, when the composite composition (compositions 1 and 2) containing both the alfalfa sprout extract, the cola sprout extract, the broccoli sprout extract, the systannium tubulosa extract and the sea tangle extract was treated, the concentration of PGE _{2 in} the effusion was lowest Respectively.

As a result of the above experiments, it is shown that the above-mentioned single extracts can be used to obtain a synergistic effect of inhibiting the production of inflammatory mediators such as NO and PGE _{2 in the} effusion as well as suppression of exudate formation due to inflammation in the body.

As described above, the present invention relates to a composition comprising alfalfa extract and cola extract, and further comprising at least one extract selected from the group consisting of broccoli extract, suthethin tofurosa extract and kelp extract, A composition for inflammation and a cosmetic composition are provided. The composition of the present invention has an effect of activating a factor promoting hair growth in the skin and increasing the expression, thereby creating a skin environment for promoting hair growth and suppressing skin irritation. And can be useful for developing functional foods and cosmetics that promote hair growth.

Claims (8)

A composition for promoting hair growth and anti-inflammatory food comprising alfalfa sprout extract and cola sprout extract.
The composition of claim 1, wherein the composition comprises alfalfa sprout extract and cola sprout extract at a weight ratio of 1: 0.1-10.
The composition according to claim 1, wherein the composition further comprises one or more extracts selected from the group consisting of broccoli extract, cis-tootrobula extract and sea tangle extract.
[4] The composition according to claim 3, wherein the composition comprises alfalfa sprout extract, colbu sprout extract, broccoli extract, Sistant body tulobos extract, and sea tangle extract in a weight ratio of 1: 0.1-10: 0.1-5: 0.1-30: &Lt; / RTI &gt;
The composition according to any one of claims 1 to 4, wherein the extract is extracted with any one solvent selected from the group consisting of water, an alcohol having 1 to 6 carbon atoms, alcohol, and a mixed solvent thereof.
A cosmetic composition for accelerating hair growth and antiinflammation comprising alfalfa sprout extract and cobra sprout extract.
[Claim 7] The composition according to claim 6, wherein the composition further comprises at least one extract selected from the group consisting of broccoli extract, cisterna tulobulosa extract and sea tangle extract.
[8] The composition according to claim 7, wherein the composition comprises alfalfa sprout extract, colbu sprout extract, broccoli extract, sistant body tulobos extract and seaweed extract at a weight ratio of 1: 0.1-10: 0.1-5: 0.1-30: &Lt; / RTI &gt;

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