JPH05229921A - Trichogenous composition - Google Patents

Abstract

PURPOSE:To provide a trichogenous composition containing a brown juice obtained from alfalfa as an active ingredient. CONSTITUTION:Alfalfa is squeezed with a press machine, and steam at 130 deg.C is fed into the obtained green juice to coagulate protein contained in the green juice. The coagulated protein is centrifugally separated, and a brown juice is obtained as the supernatant. The brown juice is added in an amount of 0.0025-0.2% (V/V), preferably 0.065-0.1% (V/V), to the trichogenous composition. The mixture of the green juice with an acid, a liquid obtained by subjecting the green juice to an acidic treatment such as a lactic acid fermentation and subsequently separating the liquid fraction from the solid fraction, and a liquid obtained by subjecting the green juice to heating treatment at 50-95 deg.C can also be used as the brown juice.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for promoting hair growth, more particularly to a composition having a hair growth promoting action, and more specifically, it is produced when a green leaf protein is extracted from alfalfa foliage. The present invention relates to a hair growth promoting composition containing a deproteinized supernatant as an active ingredient.

[0002]

Prior Art and Problems to be Solved by the Invention Alfalfa [Scientific name: Medicago L. , American name: alfalfa, English name: Lucern, medicks, Japanese name: purple palm. ] Is a plant belonging to the legume family of the perennial herb that is eaten as it is or extracted leaf protein from foliage and used as food or concentrated feed. On the other hand, it has been proposed to utilize an extract component obtained by extracting alfalfa seeds with an organic solvent agent or an oil extracted from the seeds as a cosmetic (JP-A-57-16811).

As for the juice obtained by squeezing alfalfa, it is known to use as fermented fertilizer by mixing organic fertilizer with mountain soil and clay (Modern Agriculture, Vol. 67, No. 6). , 170〜173,1988, Modern Agriculture, Vol. 6
7, No. 10, 192-195, 1988). Furthermore, the deproteinized supernatant liquid (hereinafter referred to as brown juice) that is excreted when green juice, which is juice extracted from alfalfa and the residue portion is heated and then filtered to extract green leaf protein, is heated. .) Has been conventionally tried as a yeast cell culture medium, a methane fermentation medium, an alcohol fermentation medium, a method of drying or silage with alfalfa squeezed lees, or a method of fertilizing.

However, the active ingredient of brown juice has not been sufficiently clarified, and the utilization method thereof has not been sufficiently examined, and much more effective for hair growth and hair nourishment. Nothing is known. Generally, a substance used as a hair-growth agent is desired to be safe and to have an excellent hair-growth effect in a small amount.

[0005]

[Means for Solving the Problems] As a result of intensive studies, the present inventors have found that brown
It was found that a composition containing juice as an active ingredient has a hair growth promoting action, and the present invention was completed based on this finding.

[0006] That is, the present invention provides a novel composition for promoting hair growth, which comprises a deproteinized supernatant produced when extracting alfalfa green leaf protein as an active ingredient.

The brown juice used in the present invention is a liquid produced after extracting the green leaf protein from alfalfa used as feed or food, and it is easily available, and there is no problem in safety to humans. ..

As a method for preparing the brown juice used in the present invention, for example, the raw material alfalfa is first squeezed into green juice and press cake, and the obtained green juice is heated and then solid-liquid such as filtration. It is obtained as a liquid after removing the green leaf protein by the separation operation, that is, a deproteinized supernatant. Also, dried alfalfa added with water and squeezed can be used in the same manner. The steps such as extraction of alfalfa and protein extraction from green juice may be carried out according to a conventional method, and conditions such as pressure, temperature and filtration may be appropriately determined without limitation.

The details of preparation examples of brown juice are shown below. In the present invention, the brown juice obtained according to the production method shown in the example of JP-A-3-58905 can be used. For example, the cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at 130 ° C is fed into the juice to coagulate proteins, and then the supernatant obtained by centrifugation is brown. It's juice. In addition, in order to coagulate and remove proteins contained in green juice, an acid is added to green juice, or a liquid obtained by solid-liquid separation after acid treatment such as lactic acid fermentation or the above A liquid obtained by heating green juice at 50 to 95 ° C. can also be used as brown juice. The general composition of brown juice is shown below in Table-1.

[0010]

[Table 1] Table-1 Crude protein 0.20 to 1.50% (W / W) Carbohydrate 0.30 to 2.00% (W / W) Ash content 0.03 to 0.20% (W / W) Crude fat 0.03 to 0.20% (W / W) Dry matter 1.50 to 6.00% (W / W) pH 4.0 to 6.0

The inventors of the present invention used the composition containing the brown juice obtained as described above as an active ingredient, and used the C3H mouse of an experimental animal
The experiment was performed on the back-sheared part of (week-old).
That is, after applying each sample of the experimental group in which brown juice was dissolved in an alcohol solution and the control group containing only the alcohol solution to the shaved back portion for 40 days respectively, the both were visually observed and as a result, they were exposed to the experimental group. A hair promoting effect was confirmed.

[0012] As described above, the alcohol solution containing brown juice as an active ingredient has a significant effect on promoting hair growth from the initial application, as compared with the control alcohol solution.
It has been found to be useful as a composition for promoting hair growth.

The hair growth-promoting composition of the present invention can be in various forms. For example, brown juice is purified water, higher alcohols, emulsifiers, glycols, glycerol, and liquid which are commonly used as components of hair restorers. It can be easily mixed with one or more components such as paraffin, surfactants, oils, flavors, antioxidants, etc.
Using these, a composition containing brown juice as an active ingredient can be prepared and used in a usual form such as hair oil, hair cream, hair lotion, emulsion, hairnic, shampoo.

Further, the active ingredients of the existing hair nourishing agents, for example, vitamins, amino acids, blood circulation promoters, stimulants, hormones, anti-inflammatory agents, bactericides, etc., may be appropriately mixed and used. it can. The content of brown juice which is an active ingredient in the composition of the present invention is usually 0.0
025% (v / v) to 0.2% (v / v), preferably 0.
It is suitable to set it to 05% (v / v) to 0.1% (v / v).

Hereinafter, the present invention will be specifically described with reference to test examples and the like. Test Example 1 Confirmation of hair growth promoting effect [Test method] A composition containing brown juice as an active ingredient was added to a 75% aqueous alcohol solution at a concentration of 0.0.
Test solutions were prepared by adding 25%, 0.05%, 0.1% and 0.2%. On the other hand, only a 75% alcohol solution was used as a control. As the animals used in the experiment, 45-day-old C3H mice were divided into 3 groups of 10 animals so as to have almost the same weight, and the back hair of each animal was shaved.

Next, only the test solution containing brown juice at a predetermined concentration or the control 75% alcohol solution was applied once a day for 40 days continuously to the shaved part of the back of each group of experimental animals. And compared. As a comparison method, the area value of the shaved area before application of the sample and the sample application 9 days, sample application 16 days, and sample application 23 were performed for each animal.
After 30 days from the application of the sample, 40 days after the application of the sample, the value of the area where regrowth was observed was calculated, and the ratio of the area where regrowth was recognized to the sheared area was calculated. The effect of promoting hair growth was evaluated by observing the effect of.

[Test Results] A group containing 0.025% of a composition containing brown juice as an active ingredient,
Table-2 shows the concrete change values of the ratio of the area where regeneration was observed to the shaved area of the group added with 0.05%, the group added with 0.1%, the group added with 0.2% and the control group. It was

[0018]

[Table 2]

As a result, in the group to which 0.025% of the composition containing brown juice as an active ingredient was added, a hair growth effect was recognized 40 days after application as compared with the control group. Also,
0.0% of a composition containing brown juice as an active ingredient
In comparison between the group to which 5% was added and the group to which 0.1% was added, and the control group, a hair growth effect appeared 9 days after application, and a hair growth effect was recognized even 40 days after application. However, in the group to which 0.2% of the composition containing brown juice as an active ingredient was added, although the hair growth effect was recognized at the initial stage of application, application 2
On the contrary, no effect was observed after 3 days.

From the above, the composition containing brown juice as an active ingredient is confirmed to be effective from the initial application stage to the latter stage of application, and 0.05 to 0.1% is the additive amount of brown juice. Was found to be the most preferable.

Regarding the hair cycle of the C3H mouse used in this test, it is known that it has telogen hair between 45 and 95 days after birth and human type hair that does not have an island-like independent hair cycle. And is often used as one of the evaluation methods for hair nourishing and hair-growth agents. Therefore, from the above results, it is considered that the composition containing the deproteinized supernatant produced when extracting a small amount of alfalfa green leaf protein as an active ingredient is also effective for promoting human hair growth.

[0022]

INDUSTRIAL APPLICABILITY According to the present invention, a composition containing, as an active ingredient, a brown juice obtained from alfalfa, which is easily available and used as food, concentrated feed, etc., is provided as a hair restorer. .. This product has no problem in terms of safety and can exhibit excellent effects when used at a low concentration.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Masahiro Fujimori Inventor Masahiro Fujimori 2-17, Horisaki-cho, Handa-shi, Aichi Corp Nomura Handa No. 2 Building No. 304 (72) Sumio Akita 1-23 Takada-cho, Mizuho-ku, Nagoya-shi, Aichi 3 (72) Inventor Yoshiya Kawamura 132 Furutawa, Kochino-cho, Konan-shi, Aichi

Claims (1)

[Claims] 1. A composition for promoting hair growth, which comprises, as an active ingredient, a deproteinized supernatant produced when alfalfa green leaf proteins are extracted.