KR100787640B1 - The composition for natural hair growth promoting agents - Google Patents

Abstract

The present invention relates to a hair growth accelerator composition for percutaneous application of the spray type, and an application product, which is prepared using natural ingredients, in particular ginseng and herbal medicines. Ginseng, licorice, rhubarb and ginseng are used as raw materials.

The hair growth promoter composition of the present invention promotes the production of growth hormone secretion stimulation similar to the growth hormone secretagogue hormone present in the living body using natural ingredients, ie, the production of vascular endothelial growth factor (VEGF), and prevents hair loss by ensuring stability. Of course, it may be used as a hair growth promoting agent to participate in the growth of hair root cells, as well as to delay and prevent aging.

Ginseng, hair growth promoter, VEGF

Description

  The composition for natural hair growth promoting agents

1 is a photograph showing the results of the hair growth experiment of the control group.

Figure 2 is a photograph showing the results of the hair growth experiment of the oral administration group.

Figure 3 is a photograph showing the hair growth test results of the percutaneous application and oral administration group.

Figure 4 is a photograph showing the overall hair growth test results of the control group, oral administration group, and transdermal application and oral administration group.

Figure 5 is a table showing the results of vascular endothelial growth factor (VEGF) measurement of the control group and oral administration over time.

The present invention relates to three types of hair growth promoter compositions and application products for oral administration, shampoo type transdermal application and spray type transdermal application, which are manufactured using natural ingredients, in particular ginseng and herbal ingredients. will be.

Hair loss is known to be caused by unbalanced diet, excessive drinking and smoking, drug use, lack of sleep, stress, childbirth, menopausal disorders, frequent perm and pigmentation, and hormonal imbalances. Hair loss progressed by these various causes increases the number of hair loss as the hair on the crown and forehead begins to thin and lose strength. If this phenomenon persists, hair loss becomes severe enough to be directly observed by the naked eye.

In relation to the hair regrowth agent, Korean Patent Publication No. 2003-93661, 1 ~ 25% by weight of the mixed extract of mugwort, earl pear, Sukjihwang, and Angelica, 25-35% by weight of the mixed extract of Astragalus, Seokchangpo, Ginkgo biloba, It discloses a hair growth promoter composition using a herbal medicine characterized in that 10 to 20% by weight, and 20 to 30% by weight of ginseng leaf extract.

In addition, Korean Patent Registration No. 298647 discloses willow bark, Yugeun bark, oak bark, sewage, bokbunja, licorice, peach seed, cypress leaf, and celadon bark in the order of selection, washing, cutting, sterilization and drying process. And preparing each of the golgoji, and the same amount of extraction solvent in each of the pre-treated willow bark, yugeunpi, oak bark, sesuo, bokbunja, licorice, peach seed, cypress leaf, cheonghodo bark and golgol 20 minutes to 72 hours by immersion at a temperature of 20 to 120 ℃ by adding a juice, extracting and filtration, and each extract 20 to 50% by weight of the willow bark extract, 5 to 20% by weight, extract Bark bark extract 5 to 10% by weight, sewage extract 5 to 30% by weight, bokbunja extract 5 to 20% by weight, licorice extract 5 to 20% by weight, peach seed extract 3 to 10% by weight, cypress leaf extract 5 If 20% by weight, and Chung Ho also a mixture of 3 to 20% by weight of the bark extract and extract from 5 to 20% by weight of Golgi beam disclosed for the preparation of a hair growth promoting agent characterized in that it comprises a step of obtaining a saengyakjae extract.

It is an object of the present invention to promote growth hormone secretion stimulation activity similar to growth hormone secretagogue hormone present in the living body using natural ingredients, that is, to promote the production of vascular endothelial growth factor (VEGF), and to ensure stability to prevent hair loss as well as It is to develop a natural hair growth promoter composition and its application that can be used as a hair growth promoter as well as a hair growth promoting agent by participating in the growth of hair root cells.

   In order to achieve the above object, the present invention

One step of processing 20 to 30% by weight of ginseng, 20 to 30% by weight, 20 to 30% by weight of worms and 20 to 30% by weight of fasteners into a powder or piece of 2 to 5 mm in length;

Step 2 to obtain the extract by mixing the raw material processed in the step 1 into 4 ~ 8 times of water in a dry weight ratio (ℓ / kg) for 2 to 10 hours at 60 ~ 100 ℃;

Three steps of centrifugal dehydration or filter pressing to remove solids remaining in the extracts of the two steps;

In order to remove insoluble fine particles and proteins in the extract of the three steps, the enzyme reactions of proteases, peptases, and lipases were induced at 30 to 60 ° C., and then the enzyme activity was removed at a high temperature of 90 ° C. or more, and then 15 ° C. or less. After quenching with four steps to obtain a colored transparent extract using an ultrafast centrifuge of 37,800 ~ 67,200 (g);

5 steps through the activated carbon filter to remove the fat component, phenolic compound, tannin component in the extract obtained in step 4;

6 steps of removing the inert polymer carbohydrates and microorganisms from the extract of step 5 by using a micro filtering system or an ultra filtering system; And

The extract of step 6 is concentrated by desalting several times with reverse osmosis memebrane, frozen at -40 ° C. with a lyophilizer, and then dried at elevated temperature. A hair growth promoter composition for administration is provided.

An amino acid including L-lysine and arginine may be further added to the hair growth promoter composition for oral administration.

Various foods or medicines can be prepared using the hair growth promoter composition for oral administration.

In the present invention, 10 to 20% by weight ginseng, 10 to 30% by weight Seokchangpo, licorice 15 to 25% by weight, rhubarb 15 to 25% by weight, phalanges 10 ~ 25% by weight, 10 ~ 25% by weight of ginseng powder or piece 1 step of processing and mixing;

2 steps to obtain the extract by adding the process-mixed raw materials of the first step into 4 ~ 8 times of water in a dry weight ratio (ℓ / kg) for 2 to 10 hours at 60 ~ 100 ℃;

Three steps of centrifugal dehydration or filter pressing to remove solids remaining in the extract of the two steps;

In order to remove insoluble fine particles and proteins in the extract of the three steps, the enzyme reactions of proteases, peptases, and lipases were induced at 30 to 60 ° C., and then the enzyme activity was removed at a high temperature of 90 ° C. or more, and then 15 ° C. or less. After quenching with four steps to obtain a colored transparent extract using an ultrafast centrifuge of 37,800 ~ 67,200 (g);

5 steps through the activated carbon filter to remove the fat component, phenolic compound, tannin component in the extract obtained in step 4;

6 steps of removing the inert polymer carbohydrates and microorganisms from the extract of step 5 by using a micro filtering system or an ultra filtering system; And

The extract of step 6 is desalted several times using a reverse osmosis membrane, and then concentrated primarily to 13 to 20 brix, and then, 7 steps of secondary concentration to 50 to 75 briquettes in a reduced pressure concentrator. Provided is a hair growth accelerator composition for transdermal coating of a shampoo type.

It provides any one of the hair growth promoter for transdermal application selected from shampoos, soaps, cosmetics or coatable medicines, including the shampoo-type hair growth promoter composition for transdermal application.

In addition, one step of processing 20 to 35% by weight of ginseng, licorice 15 to 30% by weight, 15-30% by weight of yellow lotus, 20 to 35% by weight of red ginseng into a powder or a piece;

Step 2 to obtain the extract by mixing the raw material processed in the step 1 in a dry weight ratio (L / kg) of 1 to 6 times 70% ethanol aqueous solution at 30 ~ 70 ℃ for 6 to 24 hours;

Separating the extract of step 2 by volatilizing alcohol using a vacuum concentrator;

In order to remove insoluble fine particles and proteins in the extract of the three steps, the enzyme reactions of proteases, peptases, and lipases were induced at 30 to 60 ° C., and then the enzyme activity was removed at a high temperature of 90 ° C. or more, and then 15 ° C. or less. After quenching with four steps to obtain a colored transparent extract using an ultrafast centrifuge of 37,800 ~ 67,200 (g);

5 steps through the activated carbon filter to remove the fat component, phenolic compound, tannin component in the extract obtained in step 4;

6 steps of removing the inert polymer carbohydrates and microorganisms using the microfiltration system or the ultrafiltration system using the extract of step 5; And

After desalting the extract of the six steps several times with a reverse osmosis membrane (desalting) to 13 ~ 20 Brix to the first concentration, and then put into a vacuum condenser to a second step to 50 ~ 75 Brix, characterized in that it comprises a seven steps Provided is a hair growth accelerator composition for transdermal application of a spray type.

In addition, the present invention is any one of the spray type selected from the spray-type cosmetics, spray-type soap, spray-type shampoo or spray-type coating medicines prepared by including the spray-type hair growth promoter composition for transdermal application A hair growth promoter for transdermal application is provided.

Hereinafter, the present invention will be described in more detail.

Ginseng, white sesame, tofu, fast protein, and polysaccharide are composed of active sugar, which is a unit substance that promotes hormone secretion and metabolic function of human body, and saponin of ginseng and asper of white sesame, tofu, and fast And saponin-like substances increase the blood flow of the human body to facilitate the nutrient supply to blood vessels in the scalp, as well as amino acids combined with the growth hormone secretion hormone stimulating growth hormone secretion activity and promote the growth hormone secretion in the anterior pituitary gland. These growth hormones produce insulin-like growth factor type (IGF-1) or vascular endothelial growth factor (VEGF) in liver or peripheral tissues, thereby promoting hair growth by promoting hair growth. It is to let. It is found that Baek Ha Shou, Toh Choong, and Sok Dan, including ginseng used in the composition of the present invention, are already toxic to the human body as herbal medicines usable as food on the Korean Food Code.

Therefore, the composition of the onset of the existing hormonal agent system that prevents hair loss by inhibiting enzymatic reaction in the scalp or an external coating hair-repellent or testosterone that helps prevent hair loss and promotes hair growth by simply nourishing existing scalp. Unlike eating hair loss inhibitors, it is possible to increase the amount of vascular endothelial growth factor, a hair follicle cell growth-promoting factor, which decreases the amount of human hair, thereby preventing the natural decline of hair root cells with a life span of about 5 years. .

In order to prepare a hair growth promoter composition for oral administration, first, raw materials of ginseng, white sewage, tofu and soybean are cut into suitable sizes, for example, 2 to 5 mm. At this time, the content of each component is 20 to 30% by weight, 20 to 30% by weight Baek Sewao, 20 to 30% by weight and two to 20 to 30% by weight fast it is preferable to use, in particular 1 to 5 in Table 1 It is most preferable to use it in five ratios.

Table 1

Preferably, the raw material is a dried material, and extracts the active ingredients in the raw material, for example, saponins and protein polysaccharides, and puts the raw material cut into the appropriate size in water and the temperature is 60 ~ 100 ℃ To match. At this time, the ratio of water and the raw material is suitable for 4 ~ 8Liter of water per 1kg of raw material, the stirring time is suitable for the range of 2 to 8 hours.

In the extract obtained after the above process, as well as the liquid from which the active ingredient is extracted, solids of ginseng, white sewage, tofu, and soybean remain as they are, and to remove them, the solids are separated using a centrifugal dehydrator or filter press.

After separating the solids, the enzyme reaction of proteases, peptases, lipases was induced between 30 ~ 60 ℃ in order to remove insoluble fine particles and proteins in the extract, and then removed the activity of the enzyme at a high temperature of more than 90 ℃ 15 ℃ After quenching to below, the solids were completely separated using an ultra-high speed centrifuge (rotating speed: 37,800 to 67,200 (g)) to obtain a colored transparent extract.

Again, an activated carbon filter is passed through the extract to remove fatty components, phenolic compounds, tannins, and the like.

Permeate and retentate are passed through an activated carbon filter using a micro filterins system and / or an ultra filtration system to remove purified polymer carbohydrates and microorganisms. After descaling several times with a nano-filtering system (nano-50, nano-90 membrane, etc.) using a reverse osmosis membrane, and concentrated after freezing at -40 ℃ with a freeze drying apparatus, the water temperature is more than 99% water soluble It is possible to produce bioactive substances (saponins, protein polysaccharides, etc.) for oral administration having a high purity hair growth promoting function, it can be used to manufacture various forms of food or medicine. It is also possible to add amino acids such as L-lysine, arginine and the like to prepare foods or pharmaceuticals.

Hereinafter will be described with respect to the shampoo-type transdermal hair growth promoter composition of another embodiment of the present invention. In the case of the shampoo-type percutaneous application hair growth promoter composition, the raw materials used are ginseng, Seokpo, licorice, rhubarb, phalanges, and ginseng. To prepare a shampoo-type percutaneous application hair growth promoter composition, dried ginseng, stone cloth, licorice, rhubarb, phalanx, and ginseng are processed into powder or piece of about 2 to 5 mm in length, and then 10 to 20% by weight of ginseng 10 to 30% by weight, licorice 15 to 25% by weight, 15 to 25% by weight of yellow lotus, 10 to 25% by weight of phalanges, 10 to 25% by weight of red ginseng is used. It is preferable that each said material uses a dried state.

In particular, it is most preferable to use the mixture in five ratios as shown in Table 2 below.

TABLE 2

In the extraction conditions suitable for the physical properties of the useful and biosynthetic components of each raw material, water is mixed at a dry weight (l / kg) ratio of about 4 to 8 times, and the heating temperature is between 60 and 100 ° C. for 2 to 10 hours. By stirring inside, ginseng mixed with the primary and useful components (saponins, protein polysaccharides, etc.) extract of each herbal medicine can be obtained.

In order to remove the solids of ginseng and herbal medicines remaining in the primary water extract using centrifugal dehydrator or filter press, the solids are separated and removed, and then proteases, peptases and lipases are used to remove insoluble particulates and proteins in the extracts. After inducing the enzyme reaction between 30 ~ 60 ℃, remove the activity of the enzyme at a high temperature of more than 90 ℃, then quenched to 15 ℃ or less, using a high-speed centrifuge (rotating speed: 37,800 ~ 67,200 (g)) The solids are separated completely to obtain a colored transparent extract.

Again, an activated carbon filter is passed through the extract to remove fatty components, phenolic compounds, tannins, and the like.

Permeate and retentate are passed through an activated carbon filter using a micro filterins system and / or an ultra filtration system to remove purified polymer carbohydrates and microorganisms. After separation, the colored transparent extract was desalted several times with a nano-filtering system using a reverse osmosis membrane and concentrated firstly up to 13-20 brix, and then put into a reduced pressure concentrator until it reached 50-75 brix. By concentrating tea, the hair growth promoter composition for transdermal application of the shampoo type can be produced.

The hair growth accelerator for transdermal application of the shampoo type can be prepared using the shampoo, soap, cosmetic or applicative drug-type hair growth promoter for the transdermal application. The manufacturing method will be omitted.

In another embodiment of the present invention to prepare a hair growth accelerator composition for the percutaneous coating of the spray type ginseng, licorice, rhubarb, ginseng 20 to 35% by weight after processing into a powder or piece of about 2 to 5mm in length, Licorice 15 to 30% by weight, sulfur ratio 15 to 30% by weight, Gosam ginseng 20 to 35% by weight of the mixture. In particular, the five ratios of Table 3 below are most preferred, and each material is preferably used in a dried state.

TABLE 3

As the extraction conditions suitable for the properties of useful ingredients and biosynthetic components of each raw material, 70% ethanol aqueous solution between 30 ℃ and 70 ℃ was introduced at 1 to 6 times by dry weight (l / kg) ratio and circulated by reflux extraction. Extract ethanol for 24 hours.

Induces the enzymatic reaction of proteases, peptases and lipases between 30 and 60 ° C to remove insoluble particulates and proteins in the extracts, which were separated by evaporating alcohol using a vacuum condenser to separate the alcohol components from the alcohol extracts. After removing the activity of the enzyme at a high temperature of 90 ℃ or more, and then quenched to 15 ℃ or less, using a high-speed centrifuge (rotating speed: 37,800 ~ 67,200 (g)) to completely separate the solid content of the colored transparent extract liquid Get

Again, an activated carbon filter is passed through the extract to remove fatty components, phenolic compounds, tannins, and the like.

Permeate and retentate are passed through an activated carbon filter using a micro filterins system and / or an ultra filtration system to remove purified polymer carbohydrates and microorganisms. And then desalting several times with a nanofiltration system using a reverse osmosis membrane to concentrate firstly up to 13-20 briquettes, and then into a reduced pressure concentrator and secondly concentrate until 50-75b briquettes are reached. A hair growth promoter composition for transdermal application can be obtained.

Spray type dermal application hair growth of any one of the spray-type cosmetics, spray-type soap, spray-type shampoo or spray-type coating medicines, including the spray-type hair growth promoter composition of the spray-type Provide accelerators.

Hereinafter, the present invention will be described through examples, and the scope of the present invention is not limited to the examples described below.

Example

(1) experimental animals

25 mice of 6 weeks old (C57BL-6) were used for experiments after adapting to breeding conditions for 1 week. The maintenance and management of the experimental animals until the experimental period was maintained under optimal conditions in the independent animal laboratory.

(2) hair growth promoter composition

A. Preparation of Hair Growth Promoter Composition for Oral Administration

Table 4

① medicinal herbs dried in the ratio of Table 4 was roughly crushed to 2 ~ 5mm thickness and mixed and put into the stirring tank.

② 6 times the volume (ℓ / g) of water compared to the dry weight of the mixed herbal medicines was added to the stirring tank and the extraction temperature was set to about 95 ℃ and the extraction process was carried out for 6 hours from the time the extraction temperature is reached.

③ When the extraction was completed, wait until the temperature of the stirring tank fell below 40 ℃, and separated the extract liquid and solid content using a filter press.

(4) A high speed centrifuge rotating at 37,800 (g) or more was passed to remove suspended solids and particulate solids remaining in the extract.

⑤ In order to remove oil components, microorganisms, heavy metals, etc. remaining in the extract, it was passed through an activated carbon filter, a micro filter and finally a nano filter, concentrated, freeze-dried and powdered.

⑥ The freeze-dried herbal extract powder and commercially available rat feed were mixed at a ratio of 30:70 to prepare pellets for easy eating.

B. Shampoo Transdermal  Preparation of hair growth promoter composition for application

Table 5

① medicinal herbs dried in the ratio of Table 5 was roughly crushed to 2 ~ 5mm thick and mixed and added to the stirring tank.

② 6 times the volume (ℓ / g) of water compared to the dry weight of the mixed herbal medicines was added to the stirring tank and the extraction temperature was set to about 95 ℃ and the extraction process was carried out for 6 hours from the time the extraction temperature is reached.

③ When the extraction was completed, wait until the temperature of the stirring tank fell below 40 ℃, and separated the extract liquid and solid content using a filter press.

(4) A high speed centrifuge rotating at 37,800 (g) or more was passed to remove suspended solids and particulate solids remaining in the extract.

⑤ To remove oil, microorganisms and heavy metals remaining in the extract, it was concentrated to 65 Brix using an activated carbon filter, a micro filter and a vacuum concentrator finally passed through the reverse osmosis membrane.

⑥ The herbal extract concentrate was mixed with distilled water at a ratio of 35:65 to prepare a sample for transdermal application of shampoo type.

 C. Preparation of hair growth accelerator composition for transdermal application of spray type

Table 6

① The medicinal herbs dried in the ratio of Table 5 were roughly crushed to a thickness of 2 to 5 mm and then put in an extracting cloth and added to a reflux tank.

② 2.5% volume (ℓ / g) 70% ethanol aqueous solution is added to the reflux tank and the extraction temperature is set to about 40 ℃, and the extraction process is carried out for 6 hours after the extraction temperature is reached. It was.

③ The ethanol was removed while maintaining the separation temperature of 75 ℃ using a vacuum condenser in order to separate the ethanol component from the ethanol aqueous solution is extracted.

(4) A high speed centrifuge rotating at 37,800 (g) or more was passed to remove suspended solids and particulate solids remaining in the extract.

⑤ To remove oil components, microorganisms and heavy metals remaining in the extract, it was concentrated to 65 brix using an activated carbon filter, a micro filter and a vacuum condenser finally passed through the nano filter.

(6) The herbal extract concentrate was mixed with ethanol at a ratio of 10:90 to prepare a spray type tread accelerator composition for transdermal application.

(3) Sample administration method

In the experiment, a total of 25 animals, 9, 8, and 8 animals were used as the control, oral, transdermal and oral groups. As each hair growth promoter composition, the hair growth promoter composition for oral administration prepared above, the hair growth promoter composition for transdermal application of the shampoo type, and the hair growth promoter composition for transdermal application of the spray type were used.

Oral administration group is a group administered the hair growth promoter composition for oral administration of the present invention, oral administration method was prepared by pellets by mixing the hair growth promoter composition for oral administration of the present invention in normal mouse feed. (60Kg adults 1 The daily dose was administered in proportion to the 20g rat weight, and the dose was weighed in the same amount as the control group so that the intake was the same (based on 5g / horse / day).

The percutaneous application and oral administration group is an experimental group to which the hair growth promoter composition for oral administration and the percutaneous application hair growth promoter composition (both shampoo type and spray type) of the present invention are applied together, and the oral administration method is the oral administration method of the oral administration group described above. In the same manner as in the above, the method of transdermal application of the shampoo-derived hair growth promoter composition of the shampoo type prepared above once a day, and then washed several times with distilled water after 5 minutes, the spray type prepared above A suitable amount of the hair growth promoter composition sample for transdermal application was sprayed and applied to the skin.

The control group and the oral administration group was washed several times with a certain amount of distilled water in order to give the same stress, and then applied a certain amount of ethanol with a spray.

(4) hair growth equipment bridge

Before the experiment, the dorsal hair was removed by short hair removal with an animal clipper. In order to uniformly suppress hair growth of all experimental animals, 1% testosterone (in ethanol) was soaked in cotton and applied to the hair removal site for 1 week. Three testers of the present invention were administered after testosterone transdermal application and liquid samples were started to be applied. The length of the grown hair was visually determined on the 49th day after sample administration and quantified by measuring the hair growth index (see Table 7). In addition, digital images were secured and visually distinguished.

   TABLE 7

Hair Growth Index 0 One 2 3 4 No growth Slight hair growth 1/3 hair growth 2/3 or more hair growth Full hair growth

According to the above criteria, the hair growth growth comparison experiment was performed for each experimental group, and the results shown in Table 8 below were obtained.

Table 8

In addition, it is shown in Table 9 when the percentage of the mouse individuals that have completely grown hair.

Table 9

In addition, photographs of each experimental group capable of confirming the results are as shown in FIGS. 1 to 4. 1 is a control group, FIG. 2 is an oral administration group, FIG. 3 is a transdermal coating and oral administration group, and FIG. 4 is an overall photograph showing all experimental groups.

   (5) Measurement of vascular endothelial growth factor (VEGF)

      Blood was collected at two hour intervals from the orally administered group and the control group's heart by direct blood collection, and the final collection time was performed after 10 hours (not tested for the transdermal and oral administration groups). Quantitative measurement of vascular endothelial growth factor (VEGF) of the collected samples was measured according to the protocol provided by purchasing an EIA (enzyme immunoassay) kit manufactured and sold by the US DSL (Diagnostic System Laboratory).

In the experiment with CHBL-6 mouse, the number of completely grown hairs increased from 4 to 5 times as shown in the attached chart, and the hair growth index was increased by 2 times (100%) as a result of comparing the hair growth index. And it was found that the hair growth promoting effect of the hair growth promoter composition of the present invention extracted in the herbal medicine is significantly.

Ginseng, Baekshou, Tobacco, and Sokcho extracts of the hair growth promoter composition for oral administration of the present invention promote growth hormone secretion stimulating activity similar to growth hormone secretagogues present in the living body, ie, production of vascular endothelial growth factor (VEGF). As it is used as a natural medicine for a long time, the stability is ensured, and it is used as a hair growth promoting agent by preventing hair loss as well as growing hair root cells. In addition, extracts of ginseng, Seokchangpo, licorice, yellow lotus, phalanx, and ginseng extracts, which are the hair growth promoting ingredients for transdermal application, remove the inflammation of the scalp and the foreign substances in the pores and the scalp, thereby removing the inflammation of the scalp cells. It can be used as a functional shampoo or cosmetics to prevent hair loss and promote hair growth by showing the effect of promoting the maintenance and growth of thick and healthy hair by supplying useful ingredients necessary for preventing hair loss and hair growth as well as making smooth contact with the hair. .

Claims (2)

20 to 35% by weight of ginseng, 15 to 30% by weight licorice, 15 to 30% by weight of rhubarb, 20 to 35% by weight of ginseng in one step of processing and mixing the powder or piece; Step 2 to obtain the extract by mixing the raw material processed in the step 1 in a dry weight ratio (L / kg) of 1 to 6 times 70% ethanol aqueous solution at 30 ~ 70 ℃ for 6 to 24 hours; Separating the extract of step 2 by volatilizing alcohol using a vacuum concentrator; In order to remove insoluble fine particles and proteins in the extract of the three steps, the enzyme reactions of proteases, peptases, and lipases were induced at 30 to 60 ° C. After quenching with four steps to obtain a colored transparent extract using an ultra-high speed centrifuge of 37,800 ~ 67,200 (g); 5 steps through the activated carbon filter to remove the fat component, phenolic compound, tannin component in the extract obtained in step 4; 6 steps of removing the inert polymer carbohydrates and microorganisms from the extract of step 5 by using a micro filtering system or an ultra filtering system; And After desalting the extract of the six steps several times with a reverse osmosis membrane (desalting) to 13 ~ 20 Brix to the first concentration, and then put into a vacuum condenser to a second step to 50 ~ 75 Brix, characterized in that it comprises a seven steps Hair growth accelerator composition for transdermal application of a spray type. The hair growth promoter for the percutaneous application of any one of the spray type selected from shampoos, soaps, cosmetics or applicative medicines, comprising the hair growth accelerator composition for the percutaneous application of the spray type of claim 1.

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