KR101702062B1 - Cosmetic composition for Preventing hair loss containing the Fermented extract of Walnuts, Chestnuts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a complex fermented extract of walnuts and chestnuts as an active ingredient.
Since the present invention uses a fermented extract of a natural product as an active ingredient, it is safe for skin, has excellent skin-affinity and absorption power, and is excellent in long-term stability, thus exhibiting an excellent effect for preventing hair loss and promoting hair growth.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for preventing hair loss using a complex fermented extract of walnuts and chestnuts,

The present invention relates to a cosmetic composition for preventing hair loss and promoting hair growth, and more particularly, to a hair loss prevention and hair growth promoting cosmetic composition containing a complex fermentation extract obtained from a mixture of walnuts and chestnuts.

In general, the causes of hair loss include genetic factors caused by hormonal effects, environmental conditions caused by air pollution, progress of aging, mental stress, and the like. In order to prevent, alleviate or treat hair loss, Various types of hair loss treatment agents have been developed by pharmacotherapy with female hormones and blood flow promoters to alleviate the phenomenon. Natural remedies using a herbal product made of natural herbal medicine extracts, far-infrared ray irradiation or physical stimulation to the scalp, Research and development on scalp management therapy to induce hair growth have also been under way.

In the field of cosmetics, the concept of eating cosmetics and functional cosmetics has been introduced, and it has evolved from the concept of simple cosmetics to expand into the field of prevention of aging and treatment of diseases, so that a lot of research has been conducted on functional cosmetics using extracts of natural products and herbal medicines have.

Recently, there has been a growing interest in hair removal products made of natural extracts which are not harmful to the human body, external preparations for preventing or alleviating hair loss by washing waste products of the scalp and supply of artificial nutrients, and natural detergents For example, Korean Patent Registration No. 10-1229762 discloses a liquid, cream, paste, or solid phase composition for improving hair growth and preventing hair loss, which comprises an extract of Hanshiku, Paekbyeop, Korean Patent Registration No. 10-1242736 discloses an external preparation for external use of skin such as lotus root, red ginseng, red ginseng, vitamin tree leaves and stem, persimmon leaves, gingiva, rosemary, black bean, black sesame, aloe, mulberry leaf, Put the herbal medicines including red bean, green tea, pine needles, peppermint, garlic, blueberry, garlic, and garlic in the aging machine at 65 ~ 95 ℃ for 3 ~ 2 Discloses a natural shampoo having a function of preventing hair loss and promoting hair growth by adding a fermentation liquid obtained by adding distilled water or softened water after aging for one day.

The present invention has accomplished the present invention by effectively extracting walnuts, nuts, unsaturated fatty acids and natural vitamins contained in chestnuts, confirming the effect of preventing hair loss and accelerating hair growth, and developing cosmetics containing active ingredients.

The present invention aims at providing a cosmetic composition for prevention of hair loss and promoting hair growth using nuts and walnuts and fermented extract of chestnuts. More specifically, the present invention relates to a walnut The present invention relates to a composition for preventing hair loss and promoting hair growth using a complex fermentation extract of chestnut and chestnut, and more particularly, for the purpose of providing a hair follicle prevention hair growth promoting hair loss prevention and hair growth using a combination fermentation extract of walnuts and chestnuts .

As a solution for achieving the object of the present invention, the cosmetic composition for prevention of hair loss and promotion of hair growth using the complex fermented extract of walnuts and chestnuts comprises 1 to 20% by weight of a fermented extract of walnuts and chestnuts based on the total weight of the cosmetic composition .

The walnut described above is a fruit of a walnut tree (Juglans sinensis), a deciduous tree of the spruce tree, and has a high taste and high nutritional value. In particular, it has been known that the protein content is higher than that of meat, the lipid has a large amount of unsaturated fatty acid, and the essential fatty acid having a function of lowering the serum cholesterol is so many that it prevents unnecessary attachment of cholesterol to blood vessels. And glaze on hair, and is said to be effective in preventing aging.

Chestnut is a chestnut tree (Castanea crenata Siebold & Zucc), which has many nutrients that can be used as food for the wilderness. Fruits contain starch, carbohydrates, fats, minerals, vitamins and lipases, It contains polyphenolic tannin, which is a polyphenolic compound that is attracting attention as a substance. It has a useful effect for anti-aging, disease prevention, fatigue recovery, and adult diseases. Especially, catechin is contained and free radical It is known that the elimination action significantly reduces the incidence of lung cancer and the like.

The combined fermentation extract of walnuts and chestnuts according to the present invention was prepared by inoculating Ganoderma lucidum into an extract obtained by extracting a mixture of walnuts and chestnuts with an organic solvent such as ethanol and fermenting at 25 ° C and pH 7 for 7 days Wherein the mixture of walnuts and chestnuts is mixed at a weight ratio of 1: 2 to 2.5 in a weight ratio of walnuts to chestnuts in a mixture of walnuts and chestnuts, and the fermented extract is prepared by separating gingko mushrooms mycelia from the resulting fermented products. .

The combined fermentation extract of walnuts and chestnuts of the present invention can be used in combination with known cosmetics with good compatibility with known cosmetics. For example, amino acids and proteins such as lecithin, Lysine PCA, sericin and water soluble collagen , Seaweed extracts such as seaweed, kelp, plant extracts such as beech tree, gingko leaf, marigold extract, humectant, and Carbopol resin Premulen resin.

The formulation of the hair loss prevention and hair growth promoting cosmetic composition using the walnut and chestnut fermentation extract of the present invention is composed of a hair shampoo or a hair toner and is manufactured according to a usual cosmetic preparation method, , A skin permeation enhancer, a colorant, a fragrance, an emulsifier, a thickener, a solvent, and the like.

The cosmetic composition for prevention of hair loss and promotion of hair growth using the complex fermented extract of walnuts and chestnuts according to the present invention is safe for the skin because of using the fermented extract of natural material as an active ingredient and has excellent skin-affinity, absorption power and long- And exhibits a very excellent effect on promotion of hair growth.

1 is an analysis graph of the fermented extract of the present invention.
Figure 2 is a chart of growth of mouse hair in all test groups after 14 days of testing.
FIG. 3 is a photograph of the growth of mouse hair in all the test groups from the 2nd week to the 4th week.
Fig. 4 is a chart of growth of hair growth on day 49 of the mouse. Fig.
Fig. 5 is a photograph of the tissue change of the skin follicles such as the second week of the mouse. Fig.
FIG. 6 is a photograph of the tissue changes of the skin follicles on the 49th day of the mouse. FIG.

Hereinafter, the present invention will be described in detail with reference to examples and test examples, but the present invention is not limited by the examples described below.

≪ Example 1 >

a). Preparation of extracts from a mixture of walnuts and chestnuts

The shredded walnuts and chestnuts (3: 7 in a mixing ratio by weight ratio) were refluxed for 3 hours in a 70% (v / v) aqueous ethanol solution for 3 hours, cooled, and filtered through Whatman # 5 filter paper The isolated extract was concentrated under reduced pressure at 50 캜 or lower and lyophilized to obtain an extract.

b). Preparation of complex fermented extract of walnuts and chestnuts

After 5% (v / v) of the mushroom mycelia was inoculated into a medium mixed with the extract obtained from the mixture of walnuts and chestnuts obtained above and yeast-malt juice (0.3-1.0% (w / v) The mixture was incubated for 6 days at a temperature of 25 캜, a rotation speed of 150 rpm, a ventilation rate of 1.5 vm, and a pH of 7.0. After cultivation, the fermented extract was prepared by removing the mycelia of the myrrh.

The mushroom mycelium was sliced on a potato dextrose agar medium, stored at 4 ° C, and subcultured every month.

≪ Test Example 1 > Measurement of total polyphenol content and total flavonoid content

The total polyphenol content and the total flavonoid content were measured to confirm the active ingredient in the biotransformed fermentation extract obtained in Example 1, and the results are shown in [Table 1] below and attached as [Figure 1]

a). Total polyphenol content measurement

To the total polyphenol content, 10 ml of distilled water was added to 1 ml of the fermented extract (sample) prepared in Example 1, 2 ml of Folin-Ciocalteu phenol reagent was added and mixed, and the mixture was reacted at room temperature for 5 minutes 2 ml of 20% sodium carbonate was added to the reaction mixture, and the mixture was reacted at room temperature for 1 hour, and the absorbance at 725 nm was measured and quantified. At this time, tannic acid was used as the indicator material.

b). Total flavonoid content measurement

To the total flavonoid content, 0.1 ml of 10% aluminum nitrate, 0.1 ml of 1 M potassium acetate and 4.3 ml of ethanol were added to 0.5 ml of the fermented extract (sample) prepared in Example 1, followed by reaction at room temperature for 40 minutes Absorbance was measured at 415 nm and quantified. At this time, quercetin was used as an indicator substance.

sample Total polyphenol content
(占 퐂 / ml) Total flavonoid content
(占 퐂 / ml) extract
(Before Biotransformation)
585
107.7 Complex fermentation extract
(After Biotransformation)
1021
589.4

As shown in the above Table 1, the total polyphenol content and total flavonoid content of the walnut and chestnut combined fermented extracts after the bioconversion increased to about 1.75 and 5.5 times, respectively, compared to the walnut and chestnut extracts before the bioconversion I can tell.

<Test Example 2> commercial and stability tests

In order to test the compatibility and stability of the complex fermented extract prepared in Example 1 above, the compatibility test was conducted with cosmetic raw materials. The compatibility was evaluated by measuring precipitation, separation, color change And the change in solubility were observed. The results are shown in Table 2 below. The temperature stability of the fermented extract was determined by measuring changes in properties and pH at various temperature conditions (room temperature, 4 ° C., 42 ° C.) After 8 weeks, it was confirmed that there was no sedimentation or denaturation, and the results are shown in Table 3 below.

Kinds of cosmetic ingredients  Raw material name Compatibility Amino acids and proteins Lecithin, Lysine PCA, sericin, water soluble collagen, etc. ○ Seaweed Seaweed, kelp, and other seaweed extracts ○ AHA series AHA, BHA, willow bark ext. ○ Other plant extracts Beech tree, gingko leaf, marigold extract, etc. ○ Other moisturizer Hyaluronic acid, etc. ○ Carbopol resin Carbopol 940, 934, 941, etc. ○ Premulen resin Permulen TR 2 ○

Temperature condition Appearance pH Other Room temperature No change 6.51
Stable without sedimentation or denaturation 42 ° C No change 6.52 4 ℃ No change 6.51

&Lt; Test Example 3 > Evaluation test for promoting hair growth

a). Test Methods

The dorsal hairs of 10-week-old C57BL / 6 mice were shortly removed with an animal clipper, and the testes were inoculated with 1% testosterone in ethanol to inhibit the hair growth of all experimental animals. After weekly application. After the testosterone transdermal application, the samples prepared as shown in [Table 4] were applied once a day to the back skin (0.2 ml / day), and the control group was washed several times with a certain amount of distilled water to give the same stress, A certain amount was applied.

Name of sample ingredient 1% TXN (Negative control) 3% MXD minoxidil (positive control) 1% TXN_EN-A Night fermented extract 1% TXN_EN-B Fermented walnut extract 1% TXN_EN-C Walnut chestnut fermented extract

On the 0th, 14th, and 49th days after the start of the experiment, photographs were taken after anesthetizing with ether to visually check the state of hair growth. The length of the hair growth was judged by the naked eye on the day 49 after the sample administration, Were measured and numerated as shown in [Table 5] below and compared with the hair growth rate. The results are shown in [FIG. 2] to [FIG. 4].

Hair growth index 0 One 2 3 4 No Growth A little hair About 1/3 hair growth More than 2/3 hair growth Full hair growth

b). Evaluation results

As shown in FIG. 2, the gonadal growth index was increased to 28.5% in the 3% MXD treated group and 28.5% in the 1% TXN control group on day 49, (P <0.05) was 3.8%, which was significantly higher than that of the control group (35.7%). In the other 1% TXN_EN-A, 1% TXN_EN-C test group, there was no difference from the control group. On the 14th day, 3% MXD treated group showed a significant increase in the growth of the hair growth index and 1% TXN_EN-B (p <0.01) in the natural application group showed a significant increase in the hair growth index compared with the 1% TXN control group And it was observed that hair growth was observed in the mice of all test groups including the 1% TXN control group after 14 days (two weeks) of the test as observed by [Figure 3].

Fig. 4 is a graph showing changes in hair growth on the 49th day based on the gross changes in the test group and the control group. Two hairs were drawn from each back of the mice and the results were measured with a burner caliper to 0.1 mm. (P <0.001) and 1% TXN_EN-C (p <0.001) in the test group, which was significantly increased more than 2 times compared to the 1% TXN control group p <0.001). As a result, 1% TXN EN-B and 1% TXN EN-C showed more hair growth promoting effect than the control group in terms of gross observation and hair growth length. . &Lt; / RTI &gt;

<Test Example 4> Histological analysis of wool

In order to observe the effect of the natural hair growth test material on the growth of the hair follicles and hair follicles after the start of the experiment, the samples were applied to the back and examined at 14 days and 49 days for histological observation of the hair growth effect. Fig. 5] and [Fig. 6].

As shown in FIG. 5, the number and size of hair follicles were increased and increased in the 3% MXD, 1% TXN_EN-B, and 1% TXN_EN-C application groups as compared with the control group. According to FIG. 6, , 3% MXD and 1% TXN_EN-B application group showed larger number and size of hair follicles than control group.

&Lt; Formulation Example 1 >

Each raw material component was separately weighed according to the composition ratios as shown in the following [Table 6], and then the components A were sequentially added to a mixing tank and stirred at 1500 rpm for 10 minutes to homogenize them. The mixture is homogenized by stirring at 50 rpm, and then charged into a container which is defoamed.

Raw material name Capacity (wt%) Remarks Walnut Noodle Complex Fermentation Extract 10.0


Component A Panthenol 0.2 Ammonium laurylethoxysulfate 30.0 Cocamidopropyl betaine 5.0 Jojoba oil 0.8 Ethylene glycol distearate 3.0 Purified water 31.46 Disodium iodide 0.03


Component B Sodium hydroxide 0.20 Ammonium lauryl sulfate 15.0 Polyquaternium-10 0.06 Mixture of methylchloroisothiazolinone / methylisothiazolinone 0.05 Combination fragrance 0.7 Cocamide dihey 3.5 Sum 100

<Formulation Example 2> Toner Hair

Each raw material component was weighed separately according to the composition ratios as shown in the following [Table 7], and the components A were sequentially added to the container and dissolved while stirring. The components B and C were sequentially added to separate vessels to homogenize the mixture. Then, a mixed solution of component C was added to the mixed solution of component B to homogenize the solution. Then, a mixed solution of component A was added thereto and dissolved while stirring. Charge the container.

Raw material name Capacity (wt%) Remarks Purified water 74.8

Component A Glycoprotein 1.50 Butylene glycol 3.00 Walnut Noodle Complex Fermentation Extract 15.0 Allantoin 0.10 PIGGY-60 Hydrogenated Castor Oil 0.20
Component B Burkwood oil 0.20 Vitamin acetate 1.00 ethanol 4.00
Component C Methylparaben 0.20 Sum 100

Claims (5)

(Ganoderma lucidum) was inoculated to the extract of the mixture of the mixture of chestnut and chestnut at a weight ratio of 3: 7, which was extracted with an organic solvent, at 25 ° C and pH 7 A method for preventing hair loss and promoting hair growth using a composite fermented extract of walnuts and chestnuts, which comprises 1 to 20% by weight of a composite fermentation extract of walnut and chestnut produced by separating the mycelium of Ganoderma lucidum from the fermentation product obtained by fermentation for 7 days Composition. delete delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is prepared from a hair follicle or a hair toner.

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