KR20120129600A - The cosmetic composition for anti-wrinkle comprising the mixture of extract of Allium cepa, Symphytum officinale, Eclipta prostrate, and Theobroma cacao - Google Patents

Abstract

PURPOSE: A composition for enhancing elasticity and wrinkle improvement which contains onion, comfrey, ecliptae herba and cacao extract is provided to increase collagen production and to suppress the activation of collagenase. CONSTITUTION: A composition for enhancing elasticity and wrinkle improvement contains onion, comfrey, ecliptae herba and cacao extract as active ingredients. The onion extract and ecliptae herba extract are contained at the mass rate of 1-10 : 1-10. A cosmetic composition for enhancing elasticity and wrinkle improvement contains onion extract, Ecliptae Herba extract and Symphytum officinale extract as the active ingredient. The onion extract, Ecliptae herba extract, and comfrey extract are contained at the mass rate of 1-10 : 1-10 : 1-10. The extract is extracted as C1-C3 of alcohol, water, or a mixture thereof. [Reference numerals] (AA) Biosynthesis amount of collagen; (BB) Biosynthesis rate of collagen(%); (C1~12) Embodiment 1-12; (DD) Retinoic acid; (EE) Negative control group; (FF) Sample

Description

The cosmetic composition for anti-wrinkle comprising the mixture of extract of Allium cepa, Symphytum officinale, Eclipta prostrate, and Theobroma cacao}

The present invention relates to a cosmetic composition for improving wrinkles, and more particularly to a cosmetic composition for improving wrinkles containing onion, comfrey, nasturtium and cacao extract and mixtures thereof having elasticity enhancing and wrinkle improving effects.

Skin is the body's primary protective layer that protects organs in the body from temperature and humidity changes, ultraviolet rays, pollutants, and other external stimuli, and plays an important role in maintaining body homeostasis. This important skin, like other organs of the human body, gradually ages as the body ages. As a result, skin elasticity loss, keratinization, wrinkle formation, and skin atrophy occur. This phenomenon of skin aging not only causes wrinkles on the surface of the skin but also changes the overall lines of the face.

Wrinkles on the skin are aging-related phenomena that represent a number of structural changes due to deterioration of biological functions. That is, it is classified into endogenous aging (intrinsicaging) that occurs with age and photoaging caused by external factors, in particular, damage caused by ultraviolet rays, in addition to endogenous aging. There may be many external factors, but the effect of ultraviolet rays in sunlight is the greatest. In the endogenous aging process, characteristic clinical skin findings are observed such as fine wrinkles, atrophy of the dermis, and reduction of subcutaneous fat layer.In the photoaging process, rough and deep wrinkles appear, abnormal elastic fiber layer material accumulates, and the skin becomes leathery and loose. .

Many studies have been conducted on the importance and role of collagen directly affecting wrinkles and skin elasticity. When collagen, which accounts for most of the skin, 77% of the skin, and 90% of the dermis, is quantitatively rich and qualitatively healthy, the skin can also be kept healthy and beautiful without wrinkles and sagging. However, the role of collagen known to date is to fill the skin to improve wrinkles and make the skin taut. This known collagen actually refers to type 1 collagen, which is the largest amount of many types of collagen. However, there are other types of collagen in the skin that are lower in content than Type 1 collagen but are clearly responsible for their important functions: Type 3, Type 4, Type 5, Type 7, and Type 17.

Conventionally, collagen was sometimes formulated into cosmetic products, but when collagen is applied to the skin surface as a cosmetic product, there is a problem in that the percutaneous absorption of collagen, which is a polymer, is difficult, and its function cannot be sufficiently expected. In addition, there was a method of injecting collagen directly into the dermis of the skin, but this method was also not a solution to improve the skin wrinkles by side effects.

Recently, retinoic acid, a protein derived from an animal placenta (Japanese Patent Laid-Open No. 8-231370), and the like have been known as substances which promote collagen synthesis. However, retinoic acid is unstable, so the formulation technology is complicated and irritating to the skin, such as the use of stability in terms of stability, animal placenta derived protein had a fatal disadvantage that can be used for the extraction of cows with mad cow disease.

Meanwhile, onion (Allium cepa) is a perennial herbaceous plant of the genus Liliumaceae. Onion varieties are divided into rounded and flat rounded scales, with red, yellow and white scales. Onions are mainly used for edible scales, and the peculiar smell of scales is known to be due to compounds such as propyl disulfide and allyl sulfide, and these compounds physiologically promote the secretion of digestive fluids and have effects such as excitement, sweating or diuresis. Reported to be present. In addition, the onion scaly stem is rich in polyphenols, and contains various vitamins, minerals such as calcium and phosphoric acid, and active ingredients such as quercetin, and is known to have excellent physiological effects such as removing harmful substances in the blood.

Also, Eclipse prostrate is a perennial herb of the Asteraceae, which is 10 ~ 70 cm high. It is a plant that occurs a lot in soybean fields in Jeju, South, and Central of Korea. It is known that the effect of promoting blood flow, analgesic, immunity, dermatitis, hair growth promotion and skin cracking dermatitis has long been known.

In addition, Symphytum officinale is a dental clinic, native to Europe, and grown for medicinal or feed purposes. Comfrey is a green vegetable that is rich in protein, vitamins, and minerals, compared to animals' livers. General ingredients are similar to other vegetables, but special ingredients include organic germanium and allantoin, which is beneficial to patients with liver disease, and has been reported to have an anticancer effect. In oriental medicine, leaves and roots are used as a medicine called gambu-ri (甘富利), which improves digestion, and is reported to be used for excessive stomach acid, gastric ulcer, anemia, boil, swelling, and dermatitis.

The cacao (Theobroma cacao) is a tree with a dicotyledonous plant mallow wall paulownia tree is native to the tropical Americas. Cacao contains a lot of polyphenols. In particular, cacao (CB) -derived polyphenols are known to be stored mainly in pigment cells of cotyledon and contain about 10% of polyphenols by weight of cacao. These cacao polyphenols are mainly composed of flavan-3-ol, such as epicatechin, procyanidin, and catechin polymers. Recent cacao-derived poly-cacao-derived polysaccharides have been shown to reduce LDL oxidation and lipoxidase activity, dose-dependent effects of chocolate consumption on plasma oxidative damage, and inhibitory effects of cocoa flavanol and procyanidine on free radical-induced red blood cell hemolysis. Research on antioxidants of phenols is being actively conducted.

Thus, the present inventors completed the present invention by finding a natural extract and its mixed composition that are effective in improving wrinkles and have no side effects.

An object of the present invention is to provide a cosmetic composition for improving elasticity and wrinkles containing onion extract and nasturtium extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for improving elasticity and wrinkles containing onion extract, nasturtium extract, comfrey extract and cacao extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for preventing skin aging containing onion extract, nasturtium extract, comfrey extract and cacao extract and mixtures thereof as an active ingredient.

In order to achieve the above object, one embodiment of the present invention provides a cosmetic composition for improving elasticity and wrinkles containing onion extract and nasturtium extract as an active ingredient.

The extract can be prepared using conventional methods known in the art, i.e., under conventional temperature and pressure conditions, using conventional solvents. Extraction solvents that can be used to prepare the onion or nasturtium extract of the present invention include, for example, extraction solvents such as water, lower alcohols having ₁ to ₃ carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3 butylene glycol May be used alone or in combination. A suitable amount of the extraction solvent is about 1 to 15 times the dry weight of onion or nasturtium, may be 5 to 12 times, and may be 10 times, but is not limited thereto. In addition, the extraction method may be used, such as heat extraction, cold needle extraction, reflux cooling extraction and ultrasonic extraction, it can be used by extracting repeatedly one or several times. In addition, the extraction temperature is not particularly limited as long as the effective activity of onion or nasturtium useful ingredient is not removed, but is preferably extracted by depositing at room temperature.

The onion extract and nasturtium extract may be used alone as cosmetics for improving wrinkles because they have a collagen production effect and a collagenase inhibitory effect. However, when the onion extract and the nasturtium extract are mixed, they have better collagen production and collagenase inhibitory effects, and therefore, it is preferable to use the mixture together (Table 3, Table 4, FIGS. 1 and 2).

The onion extract and the nasturtium extract may be contained in a weight ratio of 1 to 10: 1 to 10, and preferably contained in a weight ratio of 2 to 8: 2 to 8. However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

In addition, one embodiment of the present invention provides a cosmetic composition for improving elasticity and wrinkles containing onion extract, nasturtium extract and Comfrey extract as an active ingredient.

The Comfrey extract can also be extracted by the same extraction method to obtain an extract of onion and nasturtium.

Since the onion extract, the nasturtium extract and the extract extract each have a collagen production effect and collagenase inhibitory effect alone, it may be used alone as a cosmetic for improving wrinkles. However, when the onion extract, Nasturtium extract, and the Comfrey extract have more excellent collagen production effect and collagenase inhibition effect, it is preferable to use the mixture together (Table 3, Table 4, FIGS. 1 and 2).

The onion extract, nasturtium extract and Comfrey extract may be contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10, preferably contained in a weight ratio of 2 to 8: 2: 2 to 8: 2 to 8. . However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

In addition, one embodiment of the present invention provides a cosmetic composition for improving elasticity and wrinkles containing onion extract, nasturtium extract, Comfrey extract and cacao extract as an active ingredient.

The cacao extract may also be extracted by the same extraction method to obtain an extract of onion and nasturtium.

The onion extract, nasturtium extract, comfrey extract and cacao extract may be used alone as a cosmetic for improving wrinkles because they have collagen production effect and collagenase inhibition effect. However, when the onion extract, Nasturtium extract, and the Comfrey extract have more excellent collagen production effect and collagenase inhibition effect, it is preferable to use the mixture together (Table 3, Table 4, FIGS. 1 and 2).

The onion extract, nasturtium extract, comfrey extract and cacao extract may be contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10, and 2 to 8: 2 to 8: 2 to 8: 2: It is preferable to be contained in the weight ratio of -8. However, the content ratio may be mixed in various weight ratios according to the characteristics of the product.

In addition, one embodiment of the present invention provides a cosmetic composition for preventing skin aging containing onion extract and nasturtium extract as an active ingredient.

In addition, one embodiment of the present invention provides a skin anti-aging cosmetic composition containing an additional Comfrey extract to the onion extract and nasturtium extract, and also provides a skin anti-aging cosmetic composition containing an additional cacao extract. .

The onion extract, nasturtium extract, comfrey extract and cacao extract are also effective for enhancing skin elasticity alone. However, when the onion extract, Nasturtium extract, and the Comfrey extract have a more excellent elasticity promoting effect, it is preferable to use the mixture together (Table 5). Since skin aging can be prevented by the skin elasticity enhancing effect, the yanggi extract, nasturtium extract, comfrey extract and cacao extract alone or a mixture thereof may be usefully used as a cosmetic composition for preventing skin aging.

In addition, one embodiment of the present invention provides a cosmetic for improving wrinkles and skin elasticity containing a mixed extract of onion extract, nasturtium extract, comfrey extract and cacao extract. The mixed extract may be contained in an total weight ratio of 1 to 30% by weight, may be contained in 5 to 20% by weight.

In addition, the cosmetic composition comprising the extract of the present invention may include, in addition to the extract, conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and may be formulated with a carrier. have. Cosmetic compositions of the invention can be prepared in any formulation conventionally prepared in the art and include, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing It may be formulated as a cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, hair tonic, shampoo, rinse, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition containing onion, nasturtium, comfrey and cacao extract and mixtures thereof according to the present invention not only inhibits the activity of collagenase, but also increases collagen production, and thus can be usefully used as a cosmetic composition for improving wrinkles. have. In addition, since it is effective in enhancing skin elasticity, it may be usefully used as a cosmetic composition for improving wrinkles and enhancing elasticity.

1 is a view showing the degree of collagen biosynthesis of onion extract, nasturtium extract, comfrey extract and cacao extract and mixtures thereof.
2 is a diagram showing the degree of collagenase activity inhibition of onion extract, nasturtium extract, comfrey extract and cacao extract and mixtures thereof.

Although this invention is demonstrated in more detail based on the following Example and test example, this invention is not limited to the following Example or test example.

< Example  1> Preparation of Onion Extract

5 L was added to 1 kg of dried and chopped onions, and extracted three times at 90 ° C. to 95 ° C. for 3 hours. This was filtered through a paper filter Watman # 2 to remove solids, and the filtrate was concentrated using a reduced pressure distillation machine (EYELA, JP / N-1000) and freeze-dried to obtain 126 g of onion hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  2> Preparation of Nasturtium Extract

5 liters of tertiary distilled water was added to 500 g of dried nasturtium and heated in a bath at 75 ° C. for 24 hours. This was filtered through a paper filter Watman # 2 to remove solids, and the filtrate was concentrated using a reduced pressure distillation machine (EYELA, JP / N-1000) and then lyophilized to obtain 42 g of geothermal hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  3> Preparation of Comfrey Extract

5 L of tertiary distilled water was added to 500 g of dry compriri and heated at 75 ° C. for 24 hours. This was filtered through a paper filter Watman # 2 to remove solids, and the filtrate was concentrated using a reduced pressure distillation machine (EYELA, JP / N-1000) and then lyophilized to obtain 42 g of geothermal hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  4> Preparation of Cacao Extract

5 L of tertiary distilled water was added to 1 kg of skim cacao powder, and extracted three times at 90 ° C. to 95 ° C. for 3 hours. This was filtered through a paper filter Watman # 2 to remove solids, and the filtrate was concentrated using a reduced pressure distillation machine (EYELA, JP / N-1000) and freeze-dried to obtain 114 g of onion hydrothermal extract. The prepared extract was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy).

< Example  5-12> Preparation of Mixed Extract of Onion, Nasturtium, Comfrey and Cacao

Onion, Nasturtium, Comfrey and cacao extracts were obtained by mixing in a weight ratio as shown in Table 1 below. Specifically, each extract obtained in Examples 1 to 5 was dissolved in tertiary distilled water at a concentration of 20 mg / ml, and then mixed extracts were prepared by mixing respective extract aqueous solutions so that the weight ratio of each extract was shown in Table 1.

Weight ratio (%) of each extract component in mixed extract Example onion Nasturtium Comfrey cacao 5 50 50 0 0 6 50 0 50 0 7 50 0 0 50 8 0 50 50 0 9 0 50 0 50 10 0 0 50 50 11 35 35 30 0 12 25 25 25 25

< Example  Lotion containing mixed extract of onion, nasturtium, comfrey and cacao

To the lotion of Examples 13 to 16 was prepared in the composition of Table 2.

Compounding ingredients (content: wt%) Example 13 Example 14 Example 15 Example 16 (Control group) Purified water Balance Balance Balance Balance Example 5 Composition 10 - - - Example 11 Composition - 10 - - Example 12 Compositions - - 10 - Vegetable hydrogenated oil 1.5 Stearic acid 0.60 0.60 0.60 0.60 Glyceryl stearate 1.0 1.0 1.0 1.0 Cetearyl Alcohol 2.0 2.0 2.0 2.0 Polyglyceryl-10 Pentastearate & Behenyl Alcohol 1.0 1.0 1.0 1.0 Cetearyl Alcohol & Cetearyl Glucoside 2.0 2.0 2.0 2.0 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.5 1.5 1.5 1.5 Caprylic / Capric Triglycerides 11.0 11.0 11.0 11.0 Cyclomethicone 6.0 6.0 6.0 6.0 Preservative, incense a very small amount a very small amount a very small amount a very small amount Triethanolamine 0.1 0.1 0.1 0.1

< Experimental Example  1> Collagen biosynthesis promoting effect experiment

In order to confirm the effect of the onion, nasturtium, comfrey and cacao mixed extract used in the present invention as a skin wrinkle improver, collagen biosynthesis effect in human fibroblasts using retinoic acid as a positive control Was compared in the following manner.

First, human fibroblasts were planted on a plate with 6 wells, and then cultured for 24 hours after planting a certain number of cells. The culture was incubated for 16 hours in a serum-free medium, and 2 μg / ml, 10 μg / ml and 20 μg of test substance were added to each well containing 0.05% fetal bovine serum (FBS). After treatment at a concentration of μg / ml, it was incubated for 48 hours again.

After 48 hours, cell medium was collected and 100 μl of each sample was placed in each well of an antibody coated plate and incubated at 37 ° C. for 2 hours. Each well was washed with 400 μl of phosphate buffer solution (PBS), 100 μl of Antibody-Peroxidase conjugate solution (I) was added to each well, incubated at 37 ° C. for 1 hour, and each well again. Washed with 400 μl PBS. Here, 100 μl of substrate solution (TMBZ, 3, 3 ', 5, 5'-Tetramethyl benzidine) was added to each well and incubated for 15 minutes at room temperature. 100 μl of 1 N sulfuric acid was added to each well, followed by well mixing, followed by detection by ELISA reader and ELISA (enzyme-linked immunosorbant assay) at 450 nm. The results obtained in comparison with phosphorus retinoic acid are shown in Table 3 and FIG. 1.

In Figure 1, the negative control (negative control) when the wrinkle improving material is not added, the collagen production amount based on 100% and the relative effects on the test material onion, nasturtium, comfrey, cacao mixed extract and positive control retinoic acid It is shown.

As shown in Figure 1, the retinoic acid used showed an increase effect of 21.6% at a concentration of 10 ㎍ / ㎖. On the other hand, the mixed extract of onion and nasturtium, onion, nasturtium and comfrey mixed extract, onion, nasturtium, comfrey and cacao mixed extract showed 18.6%, 22.7%, and 28.2% increase effect at 10 ㎍ / ㎖, than retinoic acid. It was confirmed that it further promotes collagen biosynthesis.

Collagen biosynthesis promotion measurement
Collagen amount (% of control) 2 Μg / ml 10 Μg / ml 20 Μg / ml Example 1 103.2 107.6 114.7 Example 2 102.3 106.2 113.4 Example 3 101.1 105.4 113.3 Example 4 102.6 108.7 116.2 Example 5 105.2 118.6 128.5 Example 6 103.2 108.4 118.2 Example 7 102.5 109.4 119.6 Example 8 102.3 106.4 115.2 Example 9 102.1 107.6 117.3 Example 10 103.3 107.2 118.4 Example 11 105.4 122.7 138.3 Example 12 107.5 128.2 145.7 Retinoic acid 106.2 121.6 136.5 Negative control group 100 100 100

< Experimental Example  2> Collagenase ( collagenase ) Activity inhibition test

In order to confirm the effect of the onion, nasturtium, comfrey and cacao mixed extract used in the present invention as a skin wrinkle improving agent, the inhibitory effect of collagenase to break down collagen and promote wrinkle formation was tested. In order to measure the degree of inhibition of collagenase (MMP-1, matrix metal protease-1) activity, the antibody of collagenase was used. In this test, a type I collagenase assay kit was used. Absorbance was measured by ELISA using the test method described below.

First, human fibroblasts were incubated in a suitable medium at 37 ℃, 5% carbon dioxide conditions. After the test, a certain number of cells were dispensed into different culture vessels and cultured for a certain time to confirm cell attachment, and then cultured in a serum-free culture for 16 hours. Tumor necrosis factor-α (TNF-alpha; TNF-alpha; a substance that activates 2 µg / ml, 10 µg / ml, 50 µg / ml and collagenase by varying concentrations of onion, nasturtium, comfrey and cacao mixed extracts in cell culture medium; tumor necrosis factor) was added and cultured for 24 hours. Collect 100 μl of the cell medium into a 96 well tissue culture plate (Corning, USA), add enzymes as detection reagents to each well, incubate at 37 ° C. for 90 minutes, and then use an ELISA reader. Measurement was made at 450 nm. In FIG. 2, collagenase inhibition efficiency (%) was shown according to an increase in the concentration of onion, nasturtium, comfrey and cacao mixed extracts compared to when no wrinkle improvement raw material was added as a negative control group.

As shown in Figure 2, as the concentration of onion, Nasturtium, Comfrey and cacao mixed extract increased to 2 μg / ml, 10 μg / ml, 50 μg / ml, the activity of collagenase was inhibited. The highest collagenase inhibitory effect of 65.4% at ㎍ / ㎖, it was confirmed that the extract of onion, nasturtium, Comfrey and cacao inhibited the activity of collagenase.

Collagenase  Activity inhibition effect measurement
Collagenase  Inhibition of activity (%) 2 μg / ml 10 μg / ml 50 [mu] g / ml Example 1 4.3 8.1 18.4 Example 2 4.4 7.8 17.5 Example 3 2.1 7.1 15.9 Example 4 4.8 8.3 19.8 Example 5 9.6 19.7 48.7 Example 6 5.7 9.1 22.2 Example 7 5.9 9.8 23.4 Example 8 6.7 10.1 24.7 Example 9 6.9 10.5 25.2 Example 10 5.1 8.8 18.2 Example 11 11.2 23.6 54.6 Example 12 15.3 28.8 65.4 TGF -β (50 ng / Ml) 56.4%

< Experimental Example  3> improve skin elasticity

In order to test the effect of improving elasticity in the skin of a real person, the following test was carried out using the compositions of Examples 1 to 12. Eighty females in their 30s and 40s divided into four groups of 20 people each, twice a day, every morning and evening, and applied to the face for twelve weeks. Was measured, and the results are shown in Table 5 below. The results in Table 5 refer to the properties of skin viscoelasticity of the skin elasticity tester. In the case of the extract of Example 15 it was confirmed that the skin elasticity effect is significantly improved compared to the control.

Skin elasticity improvement effect measurement Experimental group Skin elasticity effect Example 13 0.69 Example 14 0.78 Example 15 0.89 Example 16 (Control) 0.12

In addition, at the end of the test, subjects were asked to fill out a questionnaire, and the subjective efficacy evaluation was conducted at the same time as the mechanical evaluation. The results are shown in Table 6.

Skin Elasticity Improvement Survey Results Applicator
Respondents (sign) Very good Good usually Inadequate Example 13 3 12 5 0 Example 14 6 11 3 0 Example 15 8 10 2 0 Example 16 (Control group) One 8 11 2

< Experimental Example  5> Evaluation of Skin Safety

In order to confirm the skin safety of the composition of the present invention, a patch test of 20 adult females and 20 males was applied to evaluate the skin safety of the composition of the present invention.

In the measurement method, the patch was removed 28 hours after the patch was attached, and the first reading was performed after 30 minutes, and the second reading was performed after 96 hours. In order to determine the intensity of skin irritation of the sample, weights were assigned according to the degree of positive reaction of the skin to obtain an average skin reactivity to visually determine the skin irritation of the sample. The results are shown in Table 7 below.

Test substance Average responsiveness Judgment grade Example 13 0.00 No stimulation Example 14 0.00 No stimulation Example 15 0.00 No stimulation Example 16 0.00 No stimulation

As shown in Table 7, it can be seen that Examples 13 to 15 and Example 16 (Control) do not irritate the skin. Therefore, the cosmetic composition of this invention can be judged to be excellent in safety with respect to skin.

< Formulation example  1> flexible lotion

According to the present invention, a preparation example of a nourishing lotion containing onion, comfrey, cacao and nasturtium mixed extract was prepared as shown in Table 8.

ingredient Content (% by weight) Onion, Comfrey, Cacao and Nasturtium Mixed Extract 4.0 Polyoxyethylene Cured Castor Oil 0.4 Glycine 3.5 Dipotassium glycyrrhizate 0.1 1,3-butylene glycol 3.0 Sodium hyaluronate 0.1 ethanol 5.0 Antioxidant 0.1 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example  2> nutrition lotion

According to the present invention, a preparation example of a nourishing lotion containing onion, comfrey, cacao and nasturtium mixed extract was prepared as shown in the following Table 9.

ingredient Content (% by weight) Onion, Comfrey, Cacao and Nasturtium Mixed Extract 4.0 glycerin 7.0 Sorbitan stearate sucrose cocoate 2.0 Mineral oil 4.0 Trioctanoine 1.0 Stearic Acid 1.0 Glyceryl stearate 0.5 Sorbitan monostearate 1.0 Dimethicone 0.5 Antioxidant 0.2 Triethanolamine 0.1 Carbomer 0.2 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example  3> nutrition essence

According to the present invention, a preparation example of a nutritional essence containing onion, compri, cacao and nasturtium mixed extract was prepared as shown in Table 10.

ingredient Content (% by weight) Onion, Comfrey, Cacao and Nasturtium Mixed Extract 7.0 glycerin 5.0 1,3-butylene glycol 2.0 Polyethylene glycol 2.0 Carbomer 1.0 Sodium hyuronate 0.1 Glycine 3.0 Polyacrylamide 2.0 Hydroxyethyl Cellulose 0.2 ethanol 3.0 Polyoxyethylene Cured Castor Oil 1.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example  4> nutrition cream

According to the present invention, a preparation example of a nutrient cream containing onion, comfrey, cacao and nasturtium mixed extract was prepared as shown in Table 11 below.

ingredient Content (% by weight) Onion, Comfrey, Cacao and Nasturtium Mixed Extract 10.0 1,3-butylene glycol 3.0 glycerin 3.0 Hydrogenated Lecithin 1.0 Octyldodecanol 3.0 Trioctanoine 2.0 Stearic Acid 1.5 Cetostearyl alcohol 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 2.0 Dimethicone 3.0 Antioxidant 0.3 Xanthan gum 0.2 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

< Formulation example  5> pack

According to the present invention, a preparation example of a pack containing onion, comfrey, cacao and nasturtium mixed extract was prepared as shown in Table 12.

ingredient Content (% by weight) Onion, Comfrey, Cacao and Nasturtium Mixed Extract 8.0 glycerin 7.0 1,3-butylene glycol 3.0 Squalene 3.0 Dimethicone 3.0 Sodium hyuronate 0.1 Glycine 2.0 Polyacrylamide 5.0 Antioxidant 0.3 Triethanolamine 0.1 EDTA 0.1 Spices 0.05 Purified water Balance

Claims (12)

Cosmetic composition for improving elasticity and wrinkles containing onion extract and nasturtium extract as an active ingredient. According to claim 1, wherein the onion extract and nasturtium extract is a cosmetic composition for improving elasticity and wrinkles is contained in a weight ratio of 1 to 10: 1 to 10. Cosmetic composition for improving elasticity and wrinkles containing onion extract, nasturtium extract and Comfrey extract as an active ingredient. According to claim 3, wherein the onion extract, nasturtium extract and Comfrey extract is contained in a weight ratio of 1 to 10: 1 to 10: 1 to 10 of the cosmetic composition for improving elasticity and wrinkles. Cosmetic composition for improving elasticity and wrinkles containing onion extract, nasturtium extract, comfrey extract and cacao extract as an active ingredient. According to claim 5, The onion extract, nasturtium extract, Comfrey extract and cacao extract is contained in a 1 to 10: 1 to 10: 1 to 10: 1 to 10 weight ratio cosmetic composition for improving elasticity and wrinkles. The cosmetic composition according to claim 1, wherein the extract is extracted using a solvent selected from the group consisting of water, C ₁ to C ₃ alcohols, and mixtures thereof. . Cosmetic composition for preventing skin aging containing onion extract, nasturtium extract as an active ingredient. The cosmetic composition for preventing skin aging according to claim 8, further comprising a Comfrey extract. The cosmetic composition for preventing skin aging according to claim 9, further comprising cacao extract. A cosmetic for improving wrinkles, comprising the composition of claim 1. According to claim 11, wherein the cosmetic cosmetics for enhancing elasticity and wrinkles is any one selected from the group consisting of soft cosmetics, nourishing cosmetics, nutrition essence, nutrition cream and pack.

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