JPH09249578A - Clinical application of crude drug extract using tyrosinase activity as index - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a crude drug extract, having stronger activating actions on tyrosinases and useful as an antileukopathic or an antipoliotic therapeutic agent by carrying out the extraction from specific 56 kinds of crude drugs. SOLUTION: This crude drug extract comprises extracts of crude drugs composed of leaf, stem, seed, fruit, flower, bulb, herb, root, rhizome or the like of Acanthopanax Cortex, Eucommiae Cortex, Lycii Fructus, Lycii Folium, steamed and sun-dried Rehmanniae Radix, Mume Fructus, Ecliptae Herba, Prunellae Spica, Mori Fructs, Inulae Flos, Cimicifugae Rhizoma, Pyrrosia lingua (Thunb.) Farwell, Forsythiae Fructus, Polygoni avicularis Herba, Foeniculi Fructs, Raphani Semen, Raphanus sativus L., Helianthus annuus L., Xanthii Fructus, Valeriana officinalis L., Mentha haplocalyx Briq., Rosmarinus officinalis L., Solidago virga-aurea L. var. leiocarpa (Benth.) A., Artemisia capillaris Thunb., Foeniculum vulgate Mill., Cucumis sativus L., Phlomis tuberosa L., Solanum melongena L., Hypericum performatum L., Fagoprum esculentum Moench., Andrographis paniculata, Stachys japonica Miq., Helianthus annuus L., Arctium lappa L., Daucus carota L. var. sativa DC., Crataegi Fructus, Crataegus pinnatifida Bunge, Polypodium niponicum Mett., Lithospermum zollingeri A. DC., Thymus vulgaris L., Elaegnus angustifolia, Dononaea viscosa L., Melilotus suaveolens Ledeb., Xanthium sibiricum Patr. ex Widd., Convolvulus arvensis L., Citrus limonia Osbeck, Caryophylli Flos, Melilotus suaveolens, petasites japonicus Fr. Schmidt, Pheonix dactylifera, of Eucommia ulmoides Oliver, Scabiosa comosa Fisch., Lamium barbatum Sieb. et Zucc., Cimicifuga simplex Wormsk., Allium cepa L. and Polygonum amphibium L. Extracts obtained with a 50% ethanol are preferred due to excellence thereof in activating activities for tyrosinases in the case of all the crude drugs.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a tyrosinase activator using one or more of the following 56 herbal extracts, and an anti-white spot therapeutic agent and / or anti-white hair containing at least one of them. Regarding therapeutic agents. Table 1 shows the names of these crude drugs
Are listed.

[Table 1]

[Table 2]

[Table 3]

[0002]

2. Description of the Related Art The present inventors have previously described caffeic acid.
acid) and its derivatives have a tyrosinase activating effect and are effective for the treatment of anti-white spots and anti-hair graying.
However, they were also found to have the following disadvantages. Therefore, the present inventors have aimed to develop a more effective therapeutic drug.

[0003]

[Problems to be Solved by the Invention] For the tyrosinase activating effect of caffeic acid or chlorogenic acid (Chlorogenic acid) and their derivatives, a concentration-action curve is required between certain concentrations, but the action decreases rather than a certain concentration. To do. That is, there is a maximum value in the action, and a stronger action cannot be obtained. This development was intended to break the bottleneck.

[0004]

[Means for Solving the Problems] Extracts of crude drugs contain a number of complex components, and have a different action from the individual actions of these components or a powerful action far exceeding the individual actions. It is known to present. Based on this experience
We turned to the tyrosinase activating action of extracts of crude drugs. Many crude drugs having caffeic acid, chlorogenic acid and their derivatives are known. The present inventors have completed the present invention by discovering that the tyrosinase activating action of these extracts is so strong that it cannot be explained by the action of caffeic acid, chlorogenic acid or its derivatives.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be specifically described with reference to the embodiments of the present invention.

Example 1 A measurement system for tyrosinase activation activity was set as shown in Table 2.
As tyrosinase, mushroom-derived Sigma manufactured by Sigma was used.

[0007]

[Table 4] * Ec: Control absorbance at A475, A580nm A2min.-A0min. Es: Sample absorbance at A475, A580nm A2min.-A0min.

Example 2 The tyrosinase activating effect of a crude drug extract containing a derivative of caffeic acid / chlorogenic acid was measured. A part of them is summarized as a graph in FIG. Extraction is 50% ethanol, 10
It was carried out with 3 solvents of 0% ethanol and ethyl acetate.
The extract with 50% ethanol was the most effective for all crude drugs.

Example 3 The activating effect of these crude drug extracts was compared with that of caffeic acid and chlorogenic acid. And the hypothesis was substantiated.
See [Table 3].

[0010]

[Table 5] * All sample concentrations were 0.1%.

Example 4 An anti-alcoholic disease therapeutic agent was prepared according to the following formulation. <Tonic> Continuous 50% ethanol extract 0.5% Carpronium chloride 1.0% Benzyl nicotinate 0.05% 50% Ethanol / buffer ^* Proper amount 100ml * Sodium citrate / HCl buffer (pH 4.8 ~ 5.0)

Example 5 An anti-baldew treatment agent was prepared according to the following formulation. <Tonic> Labyrinth 50% ethanol extract or summer hay extract 0.5% Benzyl nicotinate 0.05% Cemblyx extract 0.1% Side Kashiwa leaf extract A 0.1% (Carpronium chloride 0.1%) 50% Ethanol / buffer ^* Proper amount 100ml * Citric acid Sodium / HCl buffer (pH 4.8 ~ 5.0)

Example 6 An anti-white spot therapeutic agent was prepared according to the following formulation. <Liquid formulation> 50% ethanol extract or 0.5% carpronium chloride 3 to 5.0% 1,3-butylene glycol 40.0% 20% ethanol / buffer solution ^* Proper amount 100 ml * Sodium citrate / HCl buffer solution (pH 4.8) ~ 5.0)

Example 7 An anti-white spot disease therapeutic agent was prepared according to the following formulation. <Ointment> 50% ethanol extract with continuous scent or camouflage scent 0.5g Carpronium chloride 3.0g 1,3-Butylene glycol 20.0g Hydrophilic poloid 100g

[Brief description of drawings]

FIG. 1 is a graph showing the results of measuring the tyrosinase activation effect of crude drug extracts in Examples.

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Claims (2)

[Claims] 1. Extracts of the following 56 kinds of crude drugs having tyrosinase activating action as a powerful property: [External 1] 2. One or two of the crude drug extracts according to claim 1.
Anti-white spot and / or containing at least one species
Anti-grey hair treatment agent.