KR20100090514A - Food composition for accelerating the growth - Google Patents

Abstract

PURPOSE: A food composition for the growth promotion is provided to offer the excellent growth promotive effect without side effects, and to provide the composition to children. CONSTITUTION: A food composition for the growth promotion contains a medical herbs extract. The medical herbs extract contains 100 parts of Cervi Parvum Cornu by weight, 10~50 parts of Astragalus membranaceus by weight, 5~45 parts of Atractylodes macrocephala by weight, 5~45 parts of Hoelen alba by weight, 5~45 parts of Aurantii nobilis pericarpium by weight, 5~45 parts of Fructus Hordei germinates by weight, 5~45 parts of Angelica sinensis by weight, and 5~45 parts of Crataegus pinnatifida by weight.

Description

Food composition for growth promotion {FOOD COMPOSITION FOR ACCELERATING THE GROWTH}

The present invention relates to a food composition that provides a growth promoting effect.

Growth in children means not only an increase in height, but also an increase in the anatomical and morphological size and function of each organ, ie the development of function and differentiation into organs.

Recently, the growth and development of children and adolescents has been greatly increased due to continuous economic growth, westernization of eating habits, and improvement of nutritional status. In addition, mass media have created a social atmosphere that favors taller heights, which has resulted in an increase in inferiority in children with smaller heights. As the average height increases, most people are interested in growth.

Known treatments for growth disorders include the administration of growth hormone preparations, and ilizarov surgery. In the case of treatment of growth disorders by the use of growth hormone, various factors related to growth should be taken into account, along with pruritus, seizures, fat atrophy and hypertension, glucose intolerance, pancreatitis, systemic allergic reactions, and growth hormone antibodies. There may be side effects such as benign, cancer development, and symptoms such as feminized breast. In addition, in the treatment of growth disorders by ilizarov surgical therapy, the use of surgical therapy to forcibly lengthen both legs by a short person can be painful, expensive, and unexpected. Side effects may prevent you from walking normally.

Therefore, there is an urgent need for the development of scientifically-proven drugs and foodstuffs that can fundamentally help growth.

Accordingly, it is an object of the present invention to provide a food composition which can bring about an excellent growth promoting effect without side effects.

Therefore, the present invention provides a food composition for growth promotion comprising extracts of Astragalus, Baekchul, Baekbokyeong, Dermis, Malt, Angelica, Sansa and Deer Antler.

The food composition is based on 100 parts by weight of the antler extract, 10 to 50 parts by weight of the Astragalus extract, 5 to 45 parts by weight of the white extract, 5 to 45 parts by weight of the Baekbokyeong extract, 5 to 45 parts by weight of the dermis extract, 5 to 45 parts by weight of the malt extract, 5 to 45 parts by weight of the Angelica extract, and 5 to 45 parts by weight of the hawthorn extract.

At this time, the extract of the Astragalus, the Baekchul, the Baekbokryeong, the dermis, the malt, the Angelica, the hawthorn and the antler may be a hydrothermal extract.

In addition, in the food composition, the antler extract may be fermented with Bacillus sp. Strain, which is obtained by extracting the antler fermented with the Bacillus sp. Strain or after extracting the antler to the strain of Bacillus sp. It may be obtained by fermentation.

Food composition according to the present invention is to increase the bone length, promote the metabolism of growth plate chondrocytes, growth factors IGF-1 (insulin-like growth factor-1) and BMP-2 (bone formation Protein (bone morphogenic protein) -2) can bring about excellent growth promoting effect without side effects, such as increasing the expression of it can be particularly useful as a food material for promoting children's growth.

The food composition for promoting growth according to an embodiment of the present invention includes extracts of herbal medicines such as Astragalus, Baekchul, Baekbokyeong, Dermis, Malt, Angelica, Sansa, and Deer Antler as essential ingredients.

Astragalus membranaceus Bunge var.membranaceus is a perennial herbaceous plant belonging to the legume, or the root of the grass is peeled and dried. Roots are very long, some 80-100 cm long.

Baekchul is a dried medicinal herb of the shovel (Atractylodes ovata (Thunb.) DC. ), Tastes sweet and bitter and warm in nature. have.

Baekbok-ryeong is a white-colored Bokryeong (People's name: Poria cocos (Schw) wolf ), which is a fungal nucleus that grows in roots 3-10 years after logging pine trees. Baekbokryeong is known to be used to urinate well and to control or protect the body, such as biliary edema, swelling and dampness.

Dermis (陳皮) refers to the tangerine ( Cuntrus unshiu Markovich ) or mature rinds of related plants.

Malt pours water on barley to sprout and then dry.

Angelica gigantis Nakai (also known as Angelica gigantis Nakai ) is also known as Seunggeumcho, and is known to be excellent in blood and blood (補血) action.

Sansa is the fruit of the hawthorn ( Crataegus pinnatifida Bunge for.Pinnatifida ).

Deer antler is a medicinal herb that has been harvested and processed into young horns, which have been coagulated (not yet hardened into bone) of various buckles, such as deer and maroc.

Each herbal extract as described above in the food composition according to an embodiment of the present invention is added to about 5 to 10 times the weight of each, for example by mixing each of the raw materials of the herbal medicine or its dried product or mixed with 70 It may be obtained by hot water extraction method, alcohol extraction method or the like, which is heated at 100 ° C. for 2 to 12 hours and then filtered and the filtrate is concentrated under reduced pressure. In this case, each herbal extract is not particularly limited as long as it is by a method capable of extracting the active ingredient as much as possible, and may be in the form of an extract obtained by various extraction methods.

In particular, the antler extract in the food composition according to an embodiment of the present invention may be a fermentation product fermented with Bacillus sp . That is, the antler extract in the food composition according to an embodiment of the present invention, for example, is added to the antler 5 to 10 times the weight of water and heated at 70 to 100 ℃ for 2 to 12 hours and then filtered and the filtrate The strain of the genus Bacillus to the fermentation, for example for about 25 to 40 hours at about 25 to 40 ℃ and then filtered, the filtrate may be a fermentation product concentrated under reduced pressure, for example, a fermentation method as described above for example antler After fermentation, it may be, for example, a fermented product extracted through the extraction method as described above. As described above, in the case of fermented antler, it is possible to more easily extract the active ingredient of the antler using microorganisms in the process of fermentation, not only to activate the active ingredient, but also to facilitate digestion and absorption in the body Use efficiency can be improved.

Food composition according to an embodiment of the present invention, based on 100 parts by weight of the antler extract, 10 to 50 parts by weight of Astragalus extract, 5 to 45 parts by weight of white extract, 5 to 45 parts by weight of Baekbokyeong extract, 5 to 45 parts by weight of dermis extract 5 to 45 parts by weight of malt extract, Angelica extract 5 to 45 parts by weight, and 5 to 45 parts by weight of hawthorn extract, preferably, based on 100 parts by weight of deer antler extract, 20 to 40 parts by weight of Astragalus extract, 10 to 30 parts by weight of white extract, 10 to 30 parts by weight of Baekbokyeong extract, 10 to 30 parts by weight of dermis extract, 10 to 30 parts by weight of malt extract, 10 to 30 parts by weight of Angelica extract, and 10 to 30 parts by weight of hawthorn extract. Can be.

By mixing each herbal extract to fall within the above-mentioned range, a food composition capable of achieving the effect as desired in the present invention can be obtained.

In addition, the food composition of the present invention may further include various food additives as needed.

Food composition according to an embodiment of the present invention contains a specific combination of complex herbal extracts to grow bone length, promote the metabolism of growth plate chondrocytes, increase the expression of growth factors IGF-1 and BMP-2 It can bring about an excellent growth promoting effect without side effects such as can be especially useful as a food material for promoting children's growth.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated more concretely based on an Example. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Complex Herbal Extract-(1)

Astragalus, Baekchul, Baekbokryeong, Dermis, Malt, Angelica and Sansa were selected and washed to remove foreign substances and dried. Dried, Astragalus 26.67g, Baekchul 13.33g, Baekbokyeong 13.33g, Dermis 13.33g, Malt 13.33g, Angelica g 10g, and 10g Sansa were added to the extractor, and water was added at about 5-10 times the total weight of the raw material. After extracting by heating for a period of time, the extract was filtered with a 1 μm cartridge filter, and the filtrate was concentrated under reduced pressure for 6 hours while maintaining the filtrate at 50 ° C. or lower to 50brix. A mixed extract was obtained.

Subsequently, 100 g of the washed and dried antler was crushed and placed in an extractor, and water was added at about 5-10 times the weight of the antler, followed by heating at 100 ° C. for 2 hours, followed by filtering the extract with a 1 μm cartridge filter. Bacillus strains were added to the filtrate, fermented at 37 ° C. for 30 hours, filtered using a 0.25 μm filter, and the filtrate was concentrated under reduced pressure to 10brix to obtain a antler fermentation extract.

The mixed extract and antler fermentation extract obtained in the above was mixed in a weight ratio of 1: 1 to prepare a composite herbal extract.

Example 2 Preparation of Complex Herbal Extract-(2)

A crude herbal extract was prepared in the same manner as in Example 1, except that Astragalus, Baekchul, Baekbokyeong, Dermis, Malt, Angelica and Sansa were separately extracted and mixed. At this time, Astragalus, Baekchul, Baekbokryeong, Dermis, Malt, Angelica and Sansa extract, and antler fermentation extracts were mixed at a ratio of about 0.268: 0.133: 0.133: 0.133: 0.133: 0.1: 0.1: 1.

[Test Example] Sample administration and experimental group setting

SD rats were randomly divided into 10-12 rats in each group, and then the normal diet group (control group), herbal extract (herbal extract) and low concentration group and high concentration group were set up. Each sample was added orally to the feed daily. In the case of the control group, distilled water was orally administered, and in the case of the herbal extract administration group, the complex herbal extract prepared in Example 1 was divided orally into 80 mg / kg (low concentration group) and 400 mg / kg (high concentration group). It was.

Test Example 1 Weight Change of Rat

The same visual weight was measured every day from the start of the experiment to the end of the experiment. Weight data is used as an indicator of overall metabolism and growth. Before administration, each animal had a weight range of 92 ± 2 g in rats. The weight change of the rats of the normal diet group, herbal extract low concentration group and high concentration group, respectively, is shown in FIG. 1. As a result, the normal diet group and the herbal extract group showed steady weight gain with growth during the administration period of the test substance, and there was no statistically significant difference in body weight between the experimental groups.

Test Example 2 Effect on Iliac Length Growth

To measure the efficacy of herbal extracts on bone growth in rats, Hansson's first attempt was used. This is a method for observing normal bone metabolism by using the phenomenon that the fluorescent factor is deposited on the bone through calcium and chelate bonds. When the fluorescent factor is administered in the growth stage where bone formation is most active, the administered fluorescent factor is most deposited on the bone neoplasia at the lower part of the bone growth plate to form lines. The length of the growth was observed by fluorescence microscopy. In all experimental groups, calcein (calcein: 10 mg / kg, i.p .: Sigma, U.S.A.) was administered as a fluorescent factor 4 days after the start of the experiment.

Rats were dissected 1 day after fluorescein administration. The left and right tibia of the rats were removed and fixed in 0.1 M phosphate-buffered formalin fixative for 2 hours, then demineralized and left for 2-3 days, then protected against freezing. Soaked in 30% sucrose and kept overnight at 4 ° C. After freezing the fixed bone tissue, a sagittal section of the tibia proximal part was collected every 60 μm using a sliding microtome (HM440E, Zeiss, Germany). The collected sections were placed on a slide glass and dried, and then the length between the growth plate and the growth plate formed by the deposition of fluorescent factors in bone tissue was measured using a fluorescence microscope.

As can be seen from Figure 2, the control group 379.4 ± 7.6 μm, the herbal extract high concentration (400 mg / kg) administration group 426.5 ± 11.2 μm, the herbal extract low concentration (80 mg / kg) administration group shows a growth of 402.2 ± 7.5 μm In addition, the herbal extract-treated group had better length-promoting effect than the control group, and the high herbal extract group showed statistical significance (p <0.05).

Test Example 3 Effect on Growth Plate Chondrocyte Metabolism

As described above, every 60 μm collected sections were placed on a slide glass, dried, and stained with cresyl violet, and then the height of the growth plate was measured. The height change of the growth plate of the rat is shown in FIG. 3.

As can be seen from Figure 3, the growth plate height was 552.3 ± 9.3 μm for the control group, 613.0 ± 9.6 μm and 604.9 ± 8.9 μm for the herbal extract (400 and 80 mg / kg) administration group, respectively.

In addition, the fluorescence microscope picture of the growth plate is shown in Figure 4, A picture is the growth plate picture of the control group, B picture is the growth plate picture of the herbal extract high concentration group. Growth plate is a special tissue that explosively produces bone tissue, which is essential for growth only in infants and adolescents. Therefore, in order for growth to be promoted, the function of the growth plate should be promoted, and along with this, the structure of the growth plate is also multiplied to increase the size of the growth plate. In the upper arrow of the photograph A, there is a growth plate chondrocyte in the resting phase waiting for the growth plate chondrocytes to proliferate, and below it, the growth plate chondrocytes of the proliferative part in which the flat nucleus is clustered together are shown. Below it, the growth plate cartilage cells can be seen 2-5 times larger, which is the hypertrophy. From the lower part to the lower part of the arrow is a osteoporosis (oskeleton) is formed in the chondrocytes ossified bone. In this part, bone is formed, and after chondrocytes are removed through cellular natural death process, only bone remains and bone growth is completed. Bone length growth depends entirely on the function of the growth plate. As shown in the photograph of Figure 4, it can be seen that when the herbal extract according to the present invention (in case of B) the growth plate border is increased compared to the case of A by the proliferation of chondrocytes in the growth plate.

[Test Example 4] Effect on the expression of IGF-1 (insulin-like growth factor-1) in the growth plate

Sagittal sections proximal the tibia were collected every 40 μm using a sliding microtom (HM440E, Zeiss, Germany). For immunostaining, IGF-1 antibody (Santa Cruz Biotech, USA) was used as the primary antibody and anti-goat antibody (Vector Lab, USA) was used as the secondary antibody, respectively. Vectastain elite ABC kit (Vector Lab, USA) was used.

Specifically, the tissue sections were soaked in 0.1 M PBS (phosphate-buffered saline, pH 7.2) for 5 minutes and twice in Triton-X 100 solution (Sigma, USA) for 15 minutes. After washing twice in 15 minutes in 0.1 M PBS + 0.5% BSA (Bovine Serum Albumin, Sigma, USA) for 15 minutes, and reacted with the primary antibody overnight at room temperature. The next day, rinse twice for 15 minutes in 0.1 M PBS + 0.5% BSA, then react the sections with the secondary antibody for 60 minutes, and rinse twice again for 15 minutes with 0.1 M PBS + 0.5% BSA, followed by ABC (avidin-biotin- Peroxidase (avidin-biotin-peroxidase) complex was added at a concentration of 1:50 and reacted at room temperature for 60 minutes. Each tissue section was washed twice with 0.1M PBS for 15 minutes, and then finally 0.1M with 0.05% DAB (3.3-diaminobenzidine, Sigma, USA) and 0.03% hydrogen peroxide. Reaction with PBS. After color development, each tissue section was placed in a new 0.1 M PBS to stop the reaction and observed as a slide specimen.

The fluorescence micrograph of the rat tissue section is shown in Figure 5, the left (a, b, c, d) is a picture of the control group, the right (e, f, g, h) is a photograph of a high concentration of herbal extracts. When the expression of IGF-1 in the growth plate tissue was compared between the control group and the herbal extract group, the IGF-1 expression was weakly expressed in the resting part (a and e) and the proliferating part (b and f) in both the control group and the administration group. In (c and g) and ossification (d and h), the immunostaining was increased in the herbal extract-administered group.

[Test Example 5] Effect on the expression of BMP-2 (bone morphogenic protein-2) in growth plate

Observation was carried out in the same manner as in Test Example 4, and the primary antibody used for immunostaining was BMP-2 antibody (Santa Cruz Biotech, USA), and the secondary antibody was anti-chlorine antibody (Vector Lab, USA).

The fluorescence micrograph of the rat tissue section is shown in Figure 6, the left (a, b, c, d) is a picture of the control group, the right (e, f, g, h) is a photograph of a high concentration of herbal extracts. When the expression of BMP-2 in the growth plate tissue was compared between the control group and the herbal extract group, BMP-2 expression was weakly expressed in the resting part (a and e) and the proliferating part (b and f) in both the control group and the administration group. In (c and g) and ossification (d and h) it was observed that the BMP-2 expression was increased due to increased immunostaining in the herbal extract group.

1 is a graph of weight change over time for 5 days of rats measured in Test Example 1.

2 is a graph showing the degree of long bone growth of rats measured in Test Example 2.

3 is a graph showing the change in height of the growth plate of the rat measured in Test Example 3.

Figure 4 is a fluorescence microscope picture of the rat growth plate observed in Test Example 3, A picture is a picture of the control group, B picture is a photograph of the high concentration group of herbal extracts.

5 is a fluorescence micrograph of tissue sections observed in Test Example 4 to measure the degree of IGF-1 expression in rat growth plate, the left (a, b, c, d) is a picture of the control, the right (e, f , g, h) is a photograph of the high concentration group of herbal extracts.

6 is a fluorescence micrograph of tissue sections observed in Test Example 5 to measure the degree of BMP-2 expression in the rat growth plate, the left (a, b, c, d) is a picture of the control, the right (e, f , g, h) is a photograph of the high concentration group of herbal extracts.

Claims (5)

A food composition for growth promotion comprising extracts of Astragalus, Baekchul, Baekbokryeong, Dermis, Malt, Angelica, Sansa and Deer Antler. The method of claim 1, Based on 100 parts by weight of the antler extract, 10 to 50 parts by weight of the Astragalus extract, 5 to 45 parts by weight of the white extract, 5 to 45 parts by weight of the Baekbokyeong extract, 5 to 45 parts by weight of the dermis extract, 5 to 5 parts by weight of the malt extract. 45 parts by weight, 5 to 45 parts by weight of the Angelica extract, and 5 to 45 parts by weight of the hawthorn extract, food composition for growth promotion. The method of claim 1, The extract of the Astragalus, the Baekchul, the Baekbokryeong, the dermis, the malt, the Angelica, the hawthorn and the antler are hot water extracts, food composition for growth promotion. 4. The method according to any one of claims 1 to 3, The antler extract is fermented with Bacillus sp . The method of claim 4, wherein The antler extract is obtained by extracting the antler fermented with the strain of the genus Bacillus or after extracting the antler and fermented with the strain of the genus Bacillus, growth promoting food composition.

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