CN109568496B - Detection method of traditional Chinese medicine composition for treating diabetes - Google Patents

Detection method of traditional Chinese medicine composition for treating diabetes Download PDF

Info

Publication number
CN109568496B
CN109568496B CN201910022246.1A CN201910022246A CN109568496B CN 109568496 B CN109568496 B CN 109568496B CN 201910022246 A CN201910022246 A CN 201910022246A CN 109568496 B CN109568496 B CN 109568496B
Authority
CN
China
Prior art keywords
parts
radix
traditional chinese
chinese medicine
medicine composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910022246.1A
Other languages
Chinese (zh)
Other versions
CN109568496A (en
Inventor
陈浩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201910022246.1A priority Critical patent/CN109568496B/en
Publication of CN109568496A publication Critical patent/CN109568496A/en
Application granted granted Critical
Publication of CN109568496B publication Critical patent/CN109568496B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/34—Campanulaceae (Bellflower family)
    • A61K36/344—Codonopsis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/37—Celastraceae (Staff-tree or Bittersweet family), e.g. tripterygium or spindletree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A61K36/718—Coptis (goldthread)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/808—Scrophularia (figwort)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968—Ophiopogon (Lilyturf)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/898—Orchidaceae (Orchid family)
    • A61K36/8984—Dendrobium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
    • A61K9/703—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms characterised by shape or structure; Details concerning release liner or backing; Refillable patches; User-activated patches
    • A61K9/7038—Transdermal patches of the drug-in-adhesive type, i.e. comprising drug in the skin-adhesive layer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography

Abstract

The invention relates to a method for treating diabetes and a traditional Chinese medicine composition used in the method, in particular to a method for treating diabetes by using a traditional Chinese medicine composition, for example, a method for treating diabetes by using a traditional Chinese medicine composition external patch (which can also be called tri-jiao blood sugar reduction moxibustion). The invention also relates to a traditional Chinese medicine composition used in the method for treating diabetes, a preparation method and application of the traditional Chinese medicine composition, and a quality detection method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from medicinal materials such as codonopsis pilosula, astragalus membranaceus, radix scrophulariae, dendrobe, radix ophiopogonis and the like, and can be effectively used for treating or preventing diabetes and avoiding adverse reactions caused by diabetes chemical therapeutic agents.

Description

Detection method of traditional Chinese medicine composition for treating diabetes
Technical Field
The invention belongs to the technical field of medicines, relates to a method for treating diabetes, in particular to a method for treating diabetes by using a traditional Chinese medicine composition, and for example, relates to a method for treating diabetes by using an external patch (also called triple-jiao blood-sugar-reducing moxibustion) of the traditional Chinese medicine composition. The invention also relates to a traditional Chinese medicine composition used in the method for treating diabetes.
Background
Diabetes is a common disease, and in recent years, the onset of diabetes is on the rise. Diabetes brings great pain to patients and is difficult to treat.
Metformin is a first-line medicament for clinically reducing blood sugar, and is mainly used for treating ineffective type 2 diabetes, particularly obese type 2 diabetes, by simple diet control and physical exercise; for type 1 or type 2 diabetes, the metformin can be used together with insulin, so that the hypoglycemic effect of the insulin can be increased, the insulin dosage can be reduced, and the hypoglycemia can be prevented; the metformin can also be used together with sulfonylureas oral hypoglycemic drugs, and has synergistic effect. However, it is well known that metformin is administered in relatively large daily doses, with a maximum recommended daily dose of 2550 mg for adults, and such large doses often cause the following gastrointestinal adverse effects in clinical practice: diarrhea, nausea, vomiting, stomach distension, weakness, dyspepsia, abdominal discomfort and the like.
At present, for the treatment of diabetes, because of a lot of medicines, but the curative effect is not good, the state of an illness is repeated frequently, western medicines are taken orally frequently, the medicines are inhibited to be more, the damage to a human body is increased rapidly, the adverse reaction is obvious, and the medicines only relieve the blood sugar symptom, can not control the development of the state of the illness of diabetes, and have great side effects.
Therefore, the diabetes can be treated by combining Chinese and western medicines, mainly by using the traditional Chinese medicine, and from the aspect of traditional Chinese medicine. Diabetes is called diabetes in traditional Chinese medicine. Combining the modern famous Chinese medicine theory, diabetes can be controlled fundamentally and prevented from relapse only by improving the upper-jiao, middle-jiao, lower-jiao and regulating endocrine function and constitution.
With the development of traditional Chinese medicines in recent years, a plurality of methods for treating diabetes appear. Compared with western medicines, the Chinese medicinal composition has better treatment effect. However, there are many types of diabetes, and the diabetes should be treated according to different symptoms and causes.
Although some documents report that diabetes can be effectively treated by adopting traditional Chinese medicines, no traditional Chinese medicine treatment method which can treat diabetes to control hyperglycemia and effectively reduce the incidence rate of adverse reactions is available. Accordingly, it would be desirable in the art to provide a method of treating diabetes that achieves one or more of the above objectives.
Disclosure of Invention
The present invention aims to provide a method for treating diabetes by using traditional Chinese medicines, which is expected to treat diabetes to control hyperglycemia and effectively reduce the incidence of adverse reactions. It has been unexpectedly found that the present invention provides a Chinese medicinal composition exhibiting excellent effect of controlling hyperglycemia, and the present invention has been completed based on such findings.
Therefore, the invention provides a traditional Chinese medicine composition in a first aspect, which is prepared from the following traditional Chinese medicines:
radix Codonopsis, radix astragali, radix scrophulariae,
Dendrobium, ophiopogon root, dried rehmannia root,
Bombyx Batryticatus, Coptidis rhizoma, Notoginseng radix,
Rhizoma Dioscoreae, endothelium corneum Gigeriae Galli, cortex Cinnamomi, rhizoma Atractylodis, and ramulus Euonymi.
The traditional Chinese medicine composition according to the first aspect of the invention is prepared from the following traditional Chinese medicines in parts by weight:
5-30 parts of codonopsis pilosula, 15-30 parts of astragalus root, 15-30 parts of figwort root,
15-30 parts of dendrobium, 10-30 parts of radix ophiopogonis, 15-30 parts of radix rehmanniae recen,
5-30 parts of stiff silkworm, 20-30 parts of coptis root, 15-30 parts of pseudo-ginseng,
5-30 parts of Chinese yam, 5-30 parts of endothelium corneum gigeriae galli, 5-30 parts of cinnamon, 10-30 parts of rhizoma atractylodis and 15-30 parts of winged euonymus twig.
The traditional Chinese medicine composition according to the first aspect of the invention is prepared from the following traditional Chinese medicines in parts by weight:
10-25 parts of codonopsis pilosula, 20-25 parts of astragalus root, 20-25 parts of figwort root,
20-25 parts of dendrobium, 15-25 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen,
10-25 parts of stiff silkworm, 20-25 parts of coptis root, 20-25 parts of pseudo-ginseng,
10-25 parts of Chinese yam, 10-25 parts of endothelium corneum gigeriae galli, 10-25 parts of cinnamon, 15-25 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The traditional Chinese medicine composition according to the first aspect of the invention is prepared from the following traditional Chinese medicines in parts by weight:
15-20 parts of codonopsis pilosula, 20-25 parts of astragalus root, 20-25 parts of figwort root,
20-25 parts of dendrobium, 15-20 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen,
15-20 parts of stiff silkworm, 20-25 parts of coptis root, 20-25 parts of pseudo-ginseng,
15-20 parts of Chinese yam, 15-20 parts of endothelium corneum gigeriae galli, 15-20 parts of cinnamon, 15-20 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The traditional Chinese medicine composition according to the first aspect of the invention is prepared from the following traditional Chinese medicines in parts by weight:
18 parts of radix codonopsitis, 23 parts of astragalus root, 23 parts of figwort root,
23 parts of dendrobium, 18 parts of dwarf lilyturf tuber, 23 parts of radix rehmanniae recen,
18 parts of stiff silkworm, 23 parts of coptis root, 23 parts of pseudo-ginseng,
18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis and 23 parts of winged euonymus twig.
The traditional Chinese medicine composition according to the first aspect of the invention further comprises a pharmaceutical adjuvant.
According to the first aspect of the present invention, the pharmaceutical excipients comprise a pharmaceutical base and an absorption enhancer.
According to the first aspect of the present invention, the pharmaceutical base is vaseline.
According to the first aspect of the present invention, the absorption enhancer is azone.
The traditional Chinese medicine composition according to the first aspect of the invention is prepared by a method comprising the following steps:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving the mixture by a 150-mesh sieve, adding the mixed fine powder into a mixed solution of vaseline and azone preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain a Chinese medicinal composition in the form of ointment; optionally, the step of (a) is carried out,
(4) and (4) coating the pasty traditional Chinese medicine composition obtained in the step (3) on non-woven fabric, and covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum. The traditional Chinese medicines are physically crushed and then prepared into an external preparation, so that the effect related to blood sugar reduction recorded by the invention can be effectively realized. In the present invention, the yam used is a yam tablet (as described in the yam item on page 28 of the national pharmacopoeia 2015 edition) unless otherwise specified. The inventor discovers that when the Chinese yam, the dwarf lilyturf tuber, the pseudo-ginseng and the chicken's gizzard-membrane are ground together in the process of preparing the composition, the obtained fine powder can pass through a 150-mesh sieve, and when the Chinese yam is ground alone, the Chinese yam is ground together with the other 13 medicines, the Chinese yam is ground together with the dwarf lilyturf tuber, the Chinese yam is ground together with the pseudo-ginseng or the Chinese yam is ground together with the chicken's gizzard-membrane, the fine powder which can pass through the 150-mesh sieve cannot be obtained, and viscous substances are generated in the grinding. Surprisingly, it has been found that when the four medicines of ophiopogon root, pseudo-ginseng, Chinese yam and chicken's gizzard-skin are pulverized together, the obtained fine powder can smoothly pass through the fine powder of 150 meshes, the phenomena of sticky substances and screen blockage can not occur, and the fine powder can also be smoothly mixed when being mixed with the fine powder obtained by pulverizing the other ten medicines, and the phenomena of sticky substances and screen blockage can not occur after mixing. And it has been found that the Chinese medicinal composition prepared using the method of the present invention exhibits excellent biological effects.
The traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 20 to 40.
The traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 25 to 35.
The traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 30.
the traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 1 to 5.
The traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 2 to 4.
The traditional Chinese medicine composition according to the first aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 3.
further, the second aspect of the present invention provides a method for preparing a Chinese medicinal composition, wherein the Chinese medicinal composition is prepared from the following Chinese medicinal herbs:
radix Codonopsis, radix astragali, radix scrophulariae, herba Dendrobii, radix Ophiopogonis, radix rehmanniae, Bombyx Batryticatus, Coptidis rhizoma, Notoginseng radix, rhizoma Dioscoreae, endothelium corneum Gigeriae Galli, cortex Cinnamomi, rhizoma Atractylodis, and ramulus Euonymi;
the method comprises the following steps:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving with a 150-mesh sieve, adding the mixed fine powder into a pharmaceutical adjuvant (such as a mixed solution containing a pharmaceutical substrate and an absorption enhancer) preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain an ointment-shaped traditional Chinese medicine composition; optionally, the step of (a) is carried out,
(4) and (4) coating the pasty traditional Chinese medicine composition obtained in the step (3) on non-woven fabric, and covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum.
The method according to the second aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
5-30 parts of codonopsis pilosula, 15-30 parts of astragalus membranaceus, 15-30 parts of radix scrophulariae, 15-30 parts of dendrobe, 10-30 parts of radix ophiopogonis, 15-30 parts of radix rehmanniae recen, 5-30 parts of stiff silkworm, 20-30 parts of coptis chinensis, 15-30 parts of pseudo-ginseng, 5-30 parts of Chinese yam, 5-30 parts of endothelium corneum gigeriae galli, 5-30 parts of cinnamon, 10-30 parts of rhizoma atractylodis and 15-30 parts of winged euonymus twig.
The method according to the second aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
10-25 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-25 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 10-25 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 10-25 parts of Chinese yam, 10-25 parts of endothelium corneum gigeriae galli, 10-25 parts of cinnamon, 15-25 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The method according to the second aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
15-20 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-20 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 15-20 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 15-20 parts of Chinese yam, 15-20 parts of endothelium corneum gigeriae galli, 15-20 parts of cinnamon, 15-20 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The method according to the second aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
18 parts of codonopsis pilosula, 23 parts of astragalus membranaceus, 23 parts of radix scrophulariae, 23 parts of dendrobe, 18 parts of radix ophiopogonis, 23 parts of radix rehmanniae recen, 18 parts of stiff silkworm, 23 parts of coptis chinensis, 23 parts of pseudo-ginseng, 18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis and 23 parts of winged euonymus twig.
According to the method of the second aspect of the invention, the pharmaceutical base is petrolatum.
According to the method of the second aspect of the invention, the absorption enhancer is azone.
The method according to the second aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 20 to 40.
The method according to the second aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 25 to 35.
The method according to the second aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 30.
the method according to the second aspect of the present invention, wherein the weight ratio of the powder to azone is 100: 1 to 5.
The method according to the second aspect of the present invention, wherein the weight ratio of the powder to azone is 100: 2 to 4.
The method according to the second aspect of the present invention, wherein the weight ratio of the powder to azone is 100: 3.
further, the third aspect of the present invention provides the use of a Chinese medicinal composition for:
treating or preventing diabetes,
Reduce the incidence of adverse effects of diabetes therapeutic agents such as metformin, or
Reducing the incidence of gastrointestinal side effects of diabetes therapeutic agents such as metformin;
the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines:
radix Codonopsis, radix astragali, radix scrophulariae,
Dendrobium, ophiopogon root, dried rehmannia root,
Bombyx Batryticatus, Coptidis rhizoma, Notoginseng radix,
Rhizoma Dioscoreae, endothelium corneum Gigeriae Galli, cortex Cinnamomi, rhizoma Atractylodis, and ramulus Euonymi.
The use according to the third aspect of the invention, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight:
5-30 parts of codonopsis pilosula, 15-30 parts of astragalus membranaceus, 15-30 parts of radix scrophulariae, 15-30 parts of dendrobe, 10-30 parts of radix ophiopogonis, 15-30 parts of radix rehmanniae recen, 5-30 parts of stiff silkworm, 20-30 parts of coptis chinensis, 15-30 parts of pseudo-ginseng, 5-30 parts of Chinese yam, 5-30 parts of endothelium corneum gigeriae galli, 5-30 parts of cinnamon, 10-30 parts of rhizoma atractylodis and 15-30 parts of winged euonymus twig.
The use according to the third aspect of the invention, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight:
10-25 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-25 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 10-25 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 10-25 parts of Chinese yam, 10-25 parts of endothelium corneum gigeriae galli, 10-25 parts of cinnamon, 15-25 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The use according to the third aspect of the invention, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight:
15-20 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-20 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 15-20 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 15-20 parts of Chinese yam, 15-20 parts of endothelium corneum gigeriae galli, 15-20 parts of cinnamon, 15-20 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The use according to the third aspect of the invention, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight:
18 parts of codonopsis pilosula, 23 parts of astragalus membranaceus, 23 parts of radix scrophulariae, 23 parts of dendrobe, 18 parts of radix ophiopogonis, 23 parts of radix rehmanniae recen, 18 parts of stiff silkworm, 23 parts of coptis chinensis, 23 parts of pseudo-ginseng, 18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis and 23 parts of winged euonymus twig.
The use according to the third aspect of the invention, wherein the traditional Chinese medicine composition further comprises a pharmaceutical adjuvant.
According to the use of the third aspect of the present invention, the pharmaceutical excipients include a pharmaceutical base and an absorption enhancer.
According to the use of the third aspect of the invention, the pharmaceutical base is petrolatum.
According to the use of the third aspect of the invention, the absorption enhancer is azone.
The use according to the third aspect of the present invention, wherein the Chinese medicinal composition is prepared according to a method comprising the steps of:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving the mixture by a 150-mesh sieve, adding the mixed fine powder into a mixed solution of vaseline and azone preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain a Chinese medicinal composition in the form of ointment; optionally, the step of (a) is carried out,
(4) and (4) coating the pasty traditional Chinese medicine composition obtained in the step (3) on non-woven fabric, and covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum.
The use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 20 to 40.
The use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 25 to 35.
The use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 30.
the use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 1 to 5.
The use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 2 to 4.
The use according to the third aspect of the invention, wherein the weight ratio of the medicinal powder to the azone is 100: 3.
in the traditional Chinese medicine composition, the codonopsis pilosula is used as a monarch drug to play a therapeutic role, and as a drug expected to be used in clinic, the quality control of the traditional Chinese medicine composition is required, for example, the identification of the existence of the codonopsis pilosula in the composition is an extremely important quality detection item. Under the item of radix codonopsitis recorded on 281 page of the first edition of Chinese pharmacopoeia 2015, a method for identifying radix codonopsitis by using thin-layer chromatography is provided, and the method in the pharmacopoeia can be used for identifying whether the traditional Chinese medicinal materials are radix codonopsitis or not. In the present invention, it is necessary to identify whether the composition contains codonopsis pilosula, and if codonopsis pilosula is not added as a monarch drug, the biological effect of the composition may be seriously affected. The inventor of the present invention uses the pharmacopeia method to identify whether the codonopsis pilosula is added to the traditional Chinese medicine composition obtained by the present invention, and has surprisingly found that the existence of the codonopsis pilosula can be identified by improving the pharmacopeia method probably due to the existence of azone in the composition, otherwise, the codonopsis pilosula cannot be detected.
Therefore, the fourth aspect of the invention provides a method for detecting the quality of a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines: radix Codonopsis, radix astragali, radix scrophulariae, herba Dendrobii, radix Ophiopogonis, radix rehmanniae, Bombyx Batryticatus, Coptidis rhizoma, Notoginseng radix, rhizoma Dioscoreae, endothelium corneum Gigeriae Galli, cortex Cinnamomi, rhizoma Atractylodis, and ramulus Euonymi;
the method comprises the following steps of identifying whether the codonopsis pilosula is added in the composition or not:
(a) taking a traditional Chinese medicine composition which is equivalent to 1g of codonopsis pilosula marked by 1g, adding 25ml of methanol, carrying out ultrasonic treatment for 30 minutes, filtering, drying the filtrate by distillation, adding 15ml of water into the residue to dissolve the residue, passing the residue through a D101 type macroporous adsorption resin column (the inner diameter is 1.5cm, the column height is 10cm), eluting with 50ml of water, discarding the water solution, eluting with 50ml of 50% ethanol again, collecting the eluent, drying by distillation, and adding 1ml of methanol into the residue to dissolve the residue to obtain a sample solution;
(b) adding methanol into radix Codonopsis alkyne glycoside reference substance to obtain 1mg solution per 1ml as reference substance solution;
(c) performing thin-layer chromatography (0502 of general rules of the four parts of the national pharmacopoeia 2015), sucking 2-4 ul of a sample solution and 2ul of a reference solution, respectively dropping on the same high-efficiency silica gel G thin-layer plate, developing with n-butyl alcohol-glacial acetic acid-water (7: 1: 0.5) as a developing agent, taking out, air drying, spraying with 10% sulfuric acid ethanol solution, heating at 100 ℃ until the spots are clearly developed, and respectively inspecting under sunlight and an ultraviolet lamp (365 nm);
(d) the composition added with radix Codonopsis in the chromatogram of the test sample shows spots or highlight spots with the same color at the corresponding positions of the chromatogram of the control sample; if no corresponding spot is shown, it indicates that no radix Codonopsis has been added to the composition.
The process according to the fourth aspect of the present invention, wherein 5ml of ethyl acetate and 1ml of formic acid are further added together with methanol in the step (a). It has been surprisingly found that the false negative result of the detection of Codonopsis pilosula does not occur after the addition of both ethyl acetate and formic acid with methanol.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
5-30 parts of codonopsis pilosula, 15-30 parts of astragalus membranaceus, 15-30 parts of radix scrophulariae, 15-30 parts of dendrobe, 10-30 parts of radix ophiopogonis, 15-30 parts of radix rehmanniae recen, 5-30 parts of stiff silkworm, 20-30 parts of coptis chinensis, 15-30 parts of pseudo-ginseng, 5-30 parts of Chinese yam, 5-30 parts of endothelium corneum gigeriae galli, 5-30 parts of cinnamon, 10-30 parts of rhizoma atractylodis and 15-30 parts of winged euonymus twig.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
10-25 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-25 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 10-25 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 10-25 parts of Chinese yam, 10-25 parts of endothelium corneum gigeriae galli, 10-25 parts of cinnamon, 15-25 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
15-20 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-20 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 15-20 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 15-20 parts of Chinese yam, 15-20 parts of endothelium corneum gigeriae galli, 15-20 parts of cinnamon, 15-20 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight:
18 parts of codonopsis pilosula, 23 parts of astragalus membranaceus, 23 parts of radix scrophulariae, 23 parts of dendrobe, 18 parts of radix ophiopogonis, 23 parts of radix rehmanniae recen, 18 parts of stiff silkworm, 23 parts of coptis chinensis, 23 parts of pseudo-ginseng, 18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis and 23 parts of winged euonymus twig.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition further comprises a pharmaceutical adjuvant.
According to the method of the fourth aspect of the present invention, the pharmaceutical excipients include a pharmaceutical base and an absorption enhancer.
According to the method of the fourth aspect of the invention, the pharmaceutical base is petrolatum.
According to the method of the fourth aspect of the invention, the absorption enhancer is azone.
The method according to the fourth aspect of the present invention, wherein the Chinese medicinal composition is prepared according to a method comprising the steps of:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving the mixture by a 150-mesh sieve, adding the mixed fine powder into a mixed solution of vaseline and azone preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain a Chinese medicinal composition in the form of ointment; optionally, the step of (a) is carried out,
(4) and (4) coating the pasty traditional Chinese medicine composition obtained in the step (3) on non-woven fabric, and covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum.
The method according to the fourth aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 20 to 40.
The method according to the fourth aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 25 to 35.
The method according to the fourth aspect of the present invention, wherein the weight ratio of the medicinal powder to the vaseline is 100: 30.
the method according to the fourth aspect of the present invention, wherein the weight ratio of the powder to the azone is 100: 1 to 5.
The method according to the fourth aspect of the present invention, wherein the weight ratio of the powder to the azone is 100: 2 to 4.
The method according to the fourth aspect of the present invention, wherein the weight ratio of the powder to the azone is 100: 3.
the literature cited herein is incorporated by reference in its entirety. Any one or more of the features of any embodiment of any aspect of the invention may be combined in any other embodiment of that aspect, as well as in any embodiment of another aspect, as long as such combination does not contradict.
Various terms used in the present invention have general meanings conventionally understood by those skilled in the art, and in case of non-conformity with the general meanings, the present invention shall control.
The invention treats diabetes through a traditional Chinese medicine composition, and the traditional Chinese medicine composition is prepared into an external emplastrum, which can be called tri-jiao hypoglycemic moxibustion in the invention. The Shenque point is called the root of twelve meridians and the respiratory gate in traditional Chinese medicine; shenque acupoint is located in the middle of umbilicus and is the sea of meridians, so it has special regulation effect on internal organs. The medicine passes through the channels of internal organs, twelve meridians and eight extra meridians via the Shenque point, and reaches the focus without the antidote of liver and kidney. The diabetic patients are mainly characterized by that the triple energizer is obstructed, the functions of the digestive system of the spleen and the stomach and the renal function are weakened, the endocrine is disturbed, the function of the pancreatic island is disordered, and the sugar toxicity is generated. The triple energizer blood sugar reducing moxibustion can eliminate sugar poison and solve the pain point of insulin resistance diabetes. The monarch, minister, assistant and guide formula is a plurality of pure traditional Chinese medicines, and is matched with the nitrogen ketone moxibustion Shenque point for strong absorption, the five viscera and the six abdomens are dredged, the pancreas and the spleen are regulated, the kidney is strengthened, the aims of blood-sea point, thirst quenching, triple warmer dredging and meridian and collateral sugar poison expelling are achieved, and the blood sugar can be lowered safely and effectively.
The traditional Chinese medicine composition of the invention is applied to three important acupuncture points by triple-jiao hypoglycemic moxibustion: shenque, Xiaoke and Xuehai. Shenque acupoint: it is the origin of life, the origin of qi, the meridian qi, the viscera, and the Shenque point, which are connected and communicated with the important meridians, viscera and six viscera of the human body, so that it can play a unique therapeutic role through its particularity. From a modern medical point of view: (1) the umbilical administration does not pass through the digestive system, and less passes through the liver, thus avoiding the stimulation to the digestive tract and the damage of liver metabolism to the medicinal components, and leading the medicament to better exert the curative effect. (2) The medicine stimulates nerve endings in the umbilical region, and influences the nervous system of the body to play a role in regulation. Seen from the anatomical part, the umbilical part is close to the abdominal cavity and the pelvic cavity, and main plexuses of autonomic nerves, such as the abdominal plexus, the mesenteric plexus, the hypogastric plexus, the pelvic plexus and the like, exist in the abdominal cavity and the pelvic cavity; the most important ganglia, such as the abdominal, mesenteric, aorenrenal and mesenteric segments, govern all organs and vessels of the abdominal and pelvic cavities, including the diaphragm, liver, spleen, stomach, intestine, kidney, adrenal gland, ureter, bladder, ovary, uterus (or vas deferens) and all the vessels, so that the umbilicus has strong and rapid absorption capacity and good sensing and conduction functions. When applied to the umbilical region, various medicines suitable for the disease condition can stimulate local nerve endings, regulate the functions of autonomic nerves of the organism through reflection and conduction of a nervous system, improve the physiological activities and pathological changes of internal organs and tissues, and enhance the immunity and disease resistance of the organism, thereby achieving the purposes of building up the body, preventing and treating diseases. Diabetes points: kexing for preventing and treating diabetes (thirst). Xuehai acupoint: remove blood stasis, remove sugar toxicity, promote the growth of new blood, transport and transform blood circulation to the five viscera and six hollow organs.
In the traditional Chinese medicine composition, the codonopsis pilosula, the astragalus and the radix scrophulariae are monarch drugs; the radix codonopsitis can tonify middle-jiao and qi, promote the production of body fluid and nourish blood, the radix astragali can tonify qi and generate yang, and is used for tonifying spleen and reducing blood sugar, and the radix scrophulariae can nourish yin, promote the production of body fluid and reduce blood sugar. Dendrobe, radix ophiopogonis and radix rehmanniae are used as ministerial drugs; the dendrobium is the head of the common sianchau, and has the functions of tonifying kidney, reducing internal heat, promoting the production of body fluid and reducing blood sugar; the dwarf lilyturf tuber moistens the lung, nourishes yin, benefits the stomach, promotes the production of body fluid and regulates blood sugar; dried rehmannia root, radix rehmanniae is used for nourishing yin, nourishing kidney and strengthening body resistance. The adjuvant drugs comprise Bombyx Batryticatus, Coptidis rhizoma, and Notoginseng radix; bombyx Batryticatus has effects of removing toxic substance and resolving hard mass; coptis root, rhizoma Coptidis clears heat and eliminates dampness, purges fire, removes toxic substance and lowers blood sugar; notoginseng radix has effects of promoting blood circulation, removing blood stasis, lowering blood sugar, and preventing and treating complications. The guiding drug is rhizoma Dioscoreae, endothelium corneum Gigeriae Galli, cortex Cinnamomi, rhizoma Atractylodis, and ramulus Euonymi; the Chinese yam has the effects of tonifying qi and yin, and tonifying lung, spleen and kidney; endothelium corneum Gigeriae Galli has effects of moistening spleen, promoting digestion, and lowering blood sugar; cortex Cinnamomi should be used for promoting blood circulation, tonifying fire, supporting yang, and invigorating kidney; rhizoma Atractylodis has the action of astringing spleen and essence, although it is dry, it can be made into short ones and then long ones with the moistening of Shen Xuan, so it has the action of prolonging and reducing blood sugar; ramulus Euonymi has effects of promoting blood circulation, removing blood stasis, and lowering blood sugar. Researches prove that the medicines are used together to reduce blood sugar, reduce internal heat, tonify lung, strengthen spleen and kidney, regulate endocrine disturbance, regulate pancreas and strengthen the transportation and transformation functions of a digestive system. Safe, convenient, green and sugar-reducing without side effect.
Metformin, a hydrochloride salt commonly used in clinic, has a chemical name of 1, 1-dimethylbiguanide hydrochloride. Clinically used for treating ineffective type 2 diabetes, especially obese type 2 diabetes, by simple diet control and physical exercise; for type 1 or type 2 diabetes, the product can be used together with insulin to increase blood sugar lowering effect of insulin, reduce insulin dosage, and prevent hypoglycemia; the product can also be used together with sulfonylureas oral hypoglycemic agents, and has synergistic effect. The common adverse reactions of metformin are gastrointestinal reactions, including diarrhea, nausea, vomiting, gastrectasia, hypodynamia, dyspepsia, abdominal discomfort and the like, and particularly, the gastrointestinal reactions are almost unavoidable as metformin needs to be taken for a long time. Metformin is a biguanide hypoglycemic agent. The concentration of insulin is not increased by stimulating islet cells, but the insulin directly acts on the metabolic process of sugar, promotes the anaerobic glycolysis of sugar, and increases the uptake and utilization of glucose by peripheral tissues such as muscles, fat and the like, thereby protecting the functions of the damaged islet cells from further damage and being beneficial to the long-term control of diabetes. Metformin inhibits the absorption of glucose in the intestinal tract, inhibits hepatic gluconeogenesis, reduces hepatic glucose output, and lowers blood sugar and glycosylated hemoglobin of diabetic patients. Metformin has no function of promoting fat synthesis, and has no obvious effect of reducing blood sugar for normal people. Compared with sulfonylurea hypoglycemic agents, the metformin does not stimulate insulin secretion, rarely causes hypoglycemia, and can play a synergistic role when being combined to improve the curative effect of reducing blood sugar. Metformin is absorbed orally mainly in the small intestine. The absolute bioavailability of 0.5 g of metformin orally administered in an empty stomach state is 50-60%. While eating slightly reduced the rate and extent of absorption of the drug. The pharmacokinetics test result of the oral product in China shows that the mean blood concentration reaching time after oral administration is 2 hours, and the half-life period of the mean plasma drug elimination is about 4 hours. Metformin hardly binds to plasma proteins, and the product can reach steady state plasma concentration within 24-48 hours when orally taken according to the common clinical dosage and administration scheme. The product is mainly excreted by kidney, and 90% of kidney is excreted within 24 hours after the product is orally taken.
Dendrobe (school name: Dendrobium nobile Lindl), also named as herba Dendrobii charm, herba Nerviliae Fordii, herba Hylotelephii Erythrosticti, Zine immortal plant, Chlorophytum comosum, Linlan, Busheng, herba Dendrobii, etc. The stem is upright, the meat is fleshy and thick, the stem is slightly flat and cylindrical, the length of the stem is 10-60 cm, and the thickness of the stem reaches 1.3 cm. The medicinal plants, sweet, light and slightly salty in nature and taste, are cold and enter stomach, kidney and lung meridians. To nourish stomach, promote the production of body fluid, nourish yin and clear heat. Can be used for treating yin deficiency and fluid deficiency, dry mouth, polydipsia, anorexia, retching, asthenic fever after disease, and dim and unclear vision. The dendrobium nobile has elegant, exquisite and lovely flowers, bright colors and aromatic smell, and is liked to be one of four ornamental foreign flowers. The herba Dendrobii contains dendrobine, dendrobiimine, dendrobine, dendrosinotine, dendrobine, 6-hydroxydendrobine, mucilaginous substance, and starch. Dendrobium moniliforme contains dendrobine, dendrobamine and N-methyl dendrobine (quaternary ammonium salt). Dendrobium officinale is the best product of dendrobium, has unique effects of nourishing yin and promoting the production of body fluid, and is popular with past doctors and medical classics. The single-column standard of the dendrobium officinale is newly added in the 2010 version of pharmacopoeia of the people's republic of China from 10 months and 1 day in 2010, and the history that the dendrobium officinale has no national standard is rewritten. In the new pharmacopoeia, inspection items such as dendrobium officinale thin-layer chromatography identification, mannose and glucose peak area ratio, impurities, water, total ash content, extract and the like are added, a polysaccharide and mannose content determination method is formulated, and a content determination method with specificity and controllable quality is formulated. The new standard can not only detect the authenticity of the dendrobium officinale, but also further control and identify the quality of the dendrobium officinale.
Coptis chinensis, school name: coptis chinensis franch, alias: the golden thread, the Chinese Thalictrum root and the rhizome of Chinese Thalictrum belong to Ranunculaceae, the golden thread belongs to perennial herb, the leaves are based and are confounded, the paper is firm, the egg-shaped triangle is formed, the three full cracks are formed, the middle split is egg-shaped diamond, the pinnate is deep cracked, the edge is provided with sharp saw teeth, and the lateral split is not equal to 2 deep cracks; the petiole is 5-12cm long. Wild or cultivated in valley cool and wet shading forest with elevation of 1000-1900 m. Has the effects of clearing heat, eliminating dampness, purging fire and removing toxicity. The taste is extremely bitter in mouth, and the common words of cloud that the user eats coptis chinensis in dumb and cannot speak out exist, namely the taste is achieved. The rhizome of Chinese goldthread is yellow, often branched, and dense with most fibrous roots. The leaves have long handles; the leaves are slightly leather, oval-shaped and triangular, the width of the leaves reaches 10cm, three full clefts are formed, the middle full cleft plate is oval-shaped and rhombic, the length of the middle full cleft plate is 3-8 cm, the width of the middle full cleft plate is 2-4 cm, the top end of the middle full cleft plate is sharp, the middle full cleft plate is provided with a thin handle with the length of 0.8-1.8 cm, 3 or 5 pairs of pinnate deep clefts are formed, the deep clefts are deepest in the lower part, the distance between the deep clefts is 2-6 mm, sharp sawteeth with thin puncture points are formed at the edges, the side full cleft plates are provided with handles with the length of 1.5-5 mm, the side full clefts are oblique; the petiole is 5-12cm long and has no hair. Scape 1-2, height 12-25 cm; the two-part or multi-part parasol inflorescence has 3-8 flowers; the bract is in a needle shape, and the three or five feathers are deeply cracked; the sepals are yellow green, oval in shape, 9-12.5 mm long and 2-3 mm wide; the petal shape or the linear shape is in the shape of a needle, the length is 5-6.5 mm, the top end is tapered, and the center is provided with a honey groove; stamen is about 20, anther is about 1 mm long, and filament is 2-5 mm long; 8-12 of carpel, slightly outward curved style. The length of the bone-whole line is 6-8 mm, and the handle is about the same as the length; 7-8 seeds, oval, about 2mm long, about 0.8 mm wide, brown. Blooming in 2-3 months and bearing fruit in 4-6 months. Rhizoma Coptidis is cool, moist, and shady, and is contraindicated for high temperature and drought. Generally, the air conditioner is distributed in a high mountain area of 1200-1800 m, and a natural environment with low temperature and high air humidity is needed. Cannot withstand strong sunlight, is favored by weak light, and thus requires shading. The soil is shallow in root, distributed in a soil layer of 5-10 cm, suitable for loose and fertile surface soil, rich in humus and deep soil, has the pH value of 5.5-6.5, and is slightly acidic. Growing mountain cold-damp forest at an altitude of 1500-2300 m, and cultivating the forest in wild or sometimes. Distributed in Sichuan, Guizhou, Hunan, Hubei and southern Shaanxi. Wild or cultivated in mountain forests or valley shadings with the altitude of 500-2000 m. Model specimens were collected from the city of Sichuan. The coptis root has the effects of clearing heat, drying dampness, purging fire and removing toxicity. Can be used for treating damp-heat distention, fullness, emesis, acid regurgitation, dysentery, jaundice, hyperpyrexia, coma, excessive heart-fire, vexation, insomnia, hematemesis, hemorrhage, conjunctival congestion, toothache, diabetes, carbuncle, furuncle, etc.; it is used externally to treat eczema, eczema and purulent ear canal. Huang Lian (Chinese goldthread) processed with wine is good at clearing heat in upper energizer. It can be used for treating conjunctival congestion and aphtha. Jiang Huang Lian can clear stomach, harmonize stomach and stop vomiting. Can be used for treating cold and heat accumulation, damp-heat obstruction, fullness and emesis. Yu Huang Lian soothes liver, harmonizes stomach and stops vomiting. Can be used for treating liver and stomach disharmony, emesis and acid regurgitation. The coptis chinensis has the following pharmacological actions: antibacterial, antifungal, antiviral, amebic, antiinflammatory, diarrhea resisting, cardiovascular, antipyretic, blood sugar lowering, blood lipid reducing, antioxidant, blood system influencing, and antiulcer effects. The rhizome of Chinese goldthread is a famous Chinese medicine 'coptis', contains berberine, coptisine, methyl coptisine, palmatine and other alkaloids, and can treat acute conjunctivitis, acute bacillary dysentery, acute gastroenteritis, hematemesis, carbuncle, furuncle, sore and ulcer. The rhizome contains berberine (berberine) 5.56% -7.25%, coptisine (coptisine), epiberberine (epierberine), berberrubine (berberrubine), palmatine (palmatine), african tetrandrine (columbamine), jatrorrhizine (jatrorrhizine), methylparaben (worenine), magnolia bark (magnoflorine), ferulic acid (ferulic acid), phellodendron ketone (obakonone) and phellodendron lactone (obactone).
Cinnamon (Latin scientific name: Cinnamomum cassia Presl) is a medium and large tree of the Lauraceae family, genus Lauraceae, with its bark tan. Leaves are alternate or nearly opposite, oblong to nearly acicular, leathery, edge cartilaginous, involuted, green, glossy, hairless, and thick and strong petioles. Axicon or near apical growth. White, split, soft and flat, long and round in the shape of anther oval, and ovate in the ovary. Oval fruit, dark purple at maturity, hairless, light cupped fruit tray. The flowering period is 6-8 months, and the fruit period is 10-12 months. Native China, India, Laos, Vietnam to Indonesia, etc. Warm climate is favored, moist is favored, and water accumulation is avoided, but excessive rain can cause rotten roots and leaves. Seedlings like yin, and mature trees can grow normally only under more sunlight. The micro-acidic or acidic sandy loam or loam with deep soil layer, loose texture, good drainage and strong permeability is required. The propagation modes comprise shoot propagation, cutting propagation and seed propagation. Bark of cinnamon is commonly used as a spice, a cooking material and a medicinal material. The wood can be used for manufacturing furniture, and the species can also be used as a landscaping tree species. Cinnamon, medium arbors; the bark is grey brown, and the old bark is as thick as 13 mm. The annual branches are cylindrical, black brown, have longitudinal fine stripes, are slightly short and soft, and the annual branches are more or less quadrangular, yellow brown, have longitudinal fine stripes and are densely covered with grayish yellow short villi. The terminal bud is small, the length is about 3 mm, the bud scale is wide and oval, the tip is tapered, and the bud scale is densely covered by gray yellow short villi. Leaves are grown alternately or nearly oppositely, the shape of an oblong to nearly needle-shaped body is long, the length is 8-16(34) cm, the width is 4-5.5(9.5) cm, the tip is sharp slightly, the base is sharp, leathery, the edge cartilaginous bone, the inner roll is green, glossy and unhairing, the lower part is light green, tarnished, dredged by yellow short villus, three branches from the base, the lateral veins are nearly oppositely, the leaf grows from 5-10 mm of the leaf base, slightly bends upwards to extend to the lower part of the leaf end and gradually disappears, the middle vein is sunken obviously above the middle vein, the lower part is protruded completely, a plurality of branch veins are arranged at one side of the leaf margin, the branch veins are arched and connected in the leaf margin, the transverse veins are wavy and nearly parallel, are 3-4 mm away, the upper part is not obvious, the lower part is protruded, and the branch veins are connected by the; the petiole is thick and 1.2-2 cm long, the ventral surface is flat or the lower part is slightly provided with a groove, and the petiole is covered by yellow short villi. The cinnamon is used as a medicine, the name of the medicinal materials is different, the bark is called cinnamon, the branches are transversely cut and called cassia twig, the twig is called cinnamon tip, the petiole is called cinnamomum zeylanicum, the fruit support is called cinnamon cup, the fruit is called cassia seed, and the primary fruit is called osmanthus fragrans or cassia bud. Cinnamon has the functions of warming spleen and stomach, tonifying kidney, dispelling cold and relieving pain, and is used for treating cold pain in waist and knees, deficiency cold stomachache, chronic dyspepsia, abdominal pain, vomiting and diarrhea, and amenorrhea due to cold. The cassia twig has the functions of inducing sweat, expelling pathogenic factors from muscles, warming and dredging channels and collaterals, and is used for treating exogenous cold, and aching pain of shoulder, arm and limb joints, and the cassia twig decoction has obvious antibacterial effect on staphylococcus aureus, typhoid bacillus and human type mycobacterium tuberculosis. Gui Zi can treat deficiency-cold stomachache. The medicinal quality of cinnamon varies according to the place and variety of origin, such as Qinghua cinnamon produced in northern Vietnam, which is better in quality, and cinnamon chews have a spicy and then sweet feeling, while most cinnamon chews produced in China have a sweet and then spicy feeling, which may be related to the difference of chemical components contained in the cinnamon chews. "Shen nong Ben Cao Jing" (Shen nong's herbal medicine) carries: the medicine has pungent and warm taste, mainly treats all diseases, nourishes spirit, harmonizes color, benefits joints, and tonifies middle-jiao and Qi. The medicines are engaged for relieving the bowels firstly, and the spirit is relieved after long-term administration, so that the body is light and not old. The face is bright and beautiful, and the eyebrows are as good as children. "compendium of materia Medica": to strengthen the muscles and bones and to activate blood vessels, all the herbs should be led, so long-term administration is not so old. "
Radix Ophiopogonis (Ophiopogon japonica (Linn. f.) Ker-Gawl.) is a perennial evergreen herb of Ophiopogon genus of Liliaceae family, with thick root, small root with oval or spindle-shaped middle or near end, short stem, clump formed by leaf base, leaf-shaped, bract-shaped, tip-tapered, spherical seed, flowering period of 5-8 months, and fruiting period of 8-9 months. The radix Ophiopogonis is also distributed in China, Japan, Vietnam and India. The cultivation is carried out in the southern China and other places. It is grown in the shady and humid place, under forest or beside stream of hillside with elevation below 2000 m. The small root tuber of dwarf lilyturf tuber is a traditional Chinese medicine and has the effects of promoting the production of body fluid, quenching thirst, moistening lung and relieving cough. The root of ophiopogon japonicus is thicker, and the middle or near-end part of ophiopogon japonicus is usually expanded into small root tuber in an oval or spindle shape; the small root is 1-1.5 cm long or longer, 5-10 mm wide and light brown yellow; the rhizome is slender, the diameter is 1-2 mm, and the node is provided with a membranous sheath. The stem is short, the leaf base forms a cluster, the shape of the grass is leaf-shaped, the length is 10-50 cm, a few is longer, the width is 1.5-3.5 mm, the stem has 3-7 veins, and the edge has fine saw teeth. Scape is 6-15(-27) cm long, usually much shorter than leaf, and the total inflorescence is 2-5 cm long, or sometimes longer, with several to more than ten flowers; the flowers grow singly or in pairs in the axilla of the bract; the bract is in a needle shape, the tip is gradually sharp, and the length of the lowest part can reach 7-8 mm; the pedicel is 3-4 mm long, and the joint is positioned above the middle part or near the middle part; the comforter piece often slightly sags but does not unfold, is needle-shaped, is about 5mm long, and is white or light purple; the anther is in a triangular shape and is in a shape of needles, and the length of the anther is 2.5-3 mm; the length of the flower pillar is about 4 mm, the width is about 1 mm, the base is wide, and the flower pillar is gradually narrow upwards. The seeds are spherical and have a diameter of 7-8 mm. The flowering period is 5-8 months, and the fruit period is 8-9 months. Therefore, the plant morphology changes greatly, for example, the density of the leaf cluster, the width of the leaf 4 and the length of the leaf are obviously different; however, the structure of the flowers is not changed much, particularly, the flower quilt sheet is only slightly opened when the flowers are full, the flower pillar base is wide, generally thick and short, and the characters such as a slightly conical shape are very consistent, and the characteristics are the main characteristics for identifying the seed. The Shennong Ben Cao Jing (Shen nong's herbal Jing) listed Ophiopogon japonicus as the top grade product for nourishing yin and moistening lung, said to be "taking for a long time to lighten body, not old but not hunger". The term "Mai Dong" from Ben Cao fen is sweet, slightly bitter and slightly cold. Moisten lung and clear heart, purge heat and promote fluid production, resolve phlegm and stop vomiting, cure cough and promote diuresis. The book of the medical science Zhongzhao xi Lu says that: it can enter stomach to nourish gastric juice, stimulate appetite, and enter spleen to help spleen to disperse essence in lung, relieve asthma and relieve cough. According to the traditional Chinese medicine, the dwarf lilyturf tuber is sweet and slightly bitter in taste and slightly cold in nature, enters the stomach, lung and heart channels, has the effects of nourishing yin and moistening lung, tonifying stomach and promoting fluid production, and clearing away heart fire and relieving restlessness, and is used for treating dry cough due to lung dryness, phthisis cough due to yin deficiency, pharyngitis and pharyngalgia, thirst due to body fluid consumption, internal heat and diabetes, insomnia due to vexation, constipation due to intestinal dryness and the like. Modern pharmacological research shows that radix ophiopogonis mainly contains ophiopogonin, steroid saponin, alkaloid, sitosterol, glucose, amino acid, vitamin and the like, and has the effects of resisting fatigue, removing free radicals, improving cellular immune function and reducing blood sugar. In addition, radix Ophiopogonis has effects of tranquilizing, hypnotizing, anti-myocardial ischemia, anti-arrhythmia, anti-tumor, etc., and especially has many effects on promoting the health of the elderly. In addition, ophiopogon root also has the functions of promoting the recovery of islet cell function, increasing hepatic glycogen and reducing blood sugar, and is a common product in Tangyou prescription. Radix Ophiopogonis can be used as substitute for tea. Proper amount of radix ophiopogonis is taken and soaked in boiled water for several times a day, so that the symptom of thirst can be effectively relieved. Some diabetics have deficiency of both qi and yin, so when drinking the radix ophiopogonis water, the radix codonopsis can be matched with a little radix codonopsis, and the effect of tonifying qi can be achieved.
Bombyx Batryticatus, named as Chinese medicine, is a lethal dry body of Beauveria bassiana (Bals.) Vuillant infected (or artificially inoculated) with larva of 4-5 th instar of Bombyx mori Linnaeus of Bombycidae. It is distributed in Jiangsu, Zhejiang, Sichuan and Guangdong provinces. Has the effects of calming endogenous wind, relieving spasm, dispelling pathogenic wind, relieving pain, eliminating phlegm and resolving masses. It is commonly used for treating liver wind with phlegm, infantile convulsion, infantile convulsion, tetanus, apoplexy, wry mouth, wind-heat headache, conjunctival congestion, pharyngalgia, rubella pruritus, and parotitis. The part of the bombyx batryticatus used as a medicine is a dry body which is killed by infecting (or artificially inoculating) white muscardine fungi to the larva of 4-5 th instar of bombyx mori. The product is slightly cylindrical and is bent and shrunk. The length is 2-5 cm, and the diameter is 0.5-0.7 cm. The surface is grayish yellow and is coated with white efflorescent hyphae and conidia. The head is round, the feet are 8 pairs, the body segment is obvious, and the tail is slightly bifurcated. Hard and brittle, easy to break, flat in section, white in outer layer, and 4 bright brown or bright black silk gland rings in the middle. Slight fishy smell. Slightly salty in taste. Bombyx Batryticatus has mild nature, salty and pungent taste. It enters liver, lung and stomach meridians. Bombyx Batryticatus has effects of calming endogenous wind, relieving spasm, dispelling pathogenic wind, relieving pain, eliminating phlegm, and resolving hard mass. Can be used for treating liver wind with phlegm, convulsive epilepsy, convulsion, infantile acute convulsion, tetanus, apoplexy, wry mouth, wind-heat headache, conjunctival congestion, pharyngalgia, rubella pruritus, and parotitis. In the application aspect, the medicine is used for treating facial paralysis and hemiplegia due to apoplexy: rhizoma Typhonii, Bombyx Batryticatus, and Scorpio by dividing into equal parts (unprocessed); grinding into fine powder; when the patient takes the medicine for one time, the patient takes the medicine with the wine and regulates the medicine with the wine, and the patient is not restricted by the wine (Qianzheng san from Yang Jia cang Fang). For treating infantile convulsions: bombyx Batryticatus and Scorpio, and radix Aconiti lateralis Preparata are processed with small amount; grinding into fine powder; when one character or half of Qian is taken, fresh ginger is added with warm water for irrigation (Ben Cao Yan Yi). For infants to gather their mouths and have trismus: two silkworm larva; is powder; it is administered with Mel and applied to the lips of children (formula of uterus Qi of children). Treating wind syndrome: dividing Bombyx Batryticatus (removing silk and mouth), rhizoma Alpiniae Officinarum, etc.; grinding into fine powder; half of the money is taken each time, the white plum tea is taken with the regulation, and the medicine is taken at the moment (a hundred-choice prescription). For acute laryngopathy complicated with swelling and pain: bombyx Batryticatus and rhizoma arisaematis, Bombyx Batryticatus and rhizoma arisaematis, and cortex Phellodendri, and cortex; grinding into fine powder; after taking one word, the powder is concocted with rhizoma Zingiberis recens natural juice, and is thrown with boiled water and quadawn to spit phlegm quickly, and it is taken occasionally (Bombyx Batryticatus powder from Weishi Jia cang Fang).
Codonopsis pilosula (trade name; Codonopsis pilosula (Franch.) Nannf.) of Campanulaceae, perennial herbs and milky. The stem base has a plurality of nodular stem marks, the root is usually hypertrophic and is spindle-shaped or spindle-shaped cylindrical, the stem is wound, sterile or the tip is attached with flowers, yellow green or yellow white, the leaves are grown on the main stem and the side branches, the leaf stems have short and thin bristles, the leaves are oval or narrow oval, the edges have wavy blunt saw teeth, the upper side is green, the lower side is gray green, the flower buds are grown on the branch ends and are grown or nearly grown with the leaf stems, the flower crowns are upward, the flower buds are broad bell-shaped, the split pieces are regular triangular, the anthers are long, the seeds are majority, the shape is oval, and the flowers and the fruits can be bloomed in 7-10. Elevation 1560-. Radix codonopsitis is a commonly used traditional tonifying medicine in China, and the radix codonopsitis produced in Shanxi Shandangshen area is used as a superior product in ancient times, so that the radix codonopsitis has the effects of tonifying middle-jiao and Qi, and tonifying spleen and lung. Radix Codonopsis has effects of enhancing immunity, dilating blood vessel, lowering blood pressure, improving microcirculation, and improving hemopoiesis function. In addition, the composition has an effect of improving leucocyte reduction caused by chemotherapy and radiotherapy. Codonopsis pilosula is sweet and neutral, and has no toxicity. Enter the channels and qi system of hand and foot. It enters spleen and lung meridians. Radix Codonopsis has effects of invigorating spleen, invigorating qi, and promoting fluid production. It can be used for treating weakness of spleen and stomach, deficiency of both qi and blood, asthenia, anorexia, thirst, chronic diarrhea, and proctoptosis. "the source of the meridian": clearing away the lung-heat. The herbal medicine from New year: tonify middle-jiao and qi, harmonize spleen and stomach, and relieve polydipsia. "gang mu shiyi" (supplement): for lung deficiency, it can tonify lung qi. The scientific folk medicine and grass are carried: a blood-enriching agent. It is suitable for treating chronic anemia, chlorosis, leukemia, adenosis, and rickets. The handbook of Chinese medicinal materials includes: it is used to treat consumptive disease, internal cold in stomach, dysentery, asthma, thirst, fever, spontaneous perspiration, female metrorrhagia, and puerperal diseases. The clinical application of the codonopsis pilosula is as follows: bazhen Tang (Ruizhutang Jing empirical prescription) is indicated for deficiency of both qi and blood. Pale or sallow complexion, dizziness, lassitude of the limbs, shortness of breath, no speaking desire, palpitation, poor appetite, pale tongue with thin and white coating, thready and weak pulse or weak and weak pulse. 30g of angelica sinensis, 30g of ligusticum wallichii, 30g of prepared rehmannia root, 30g of white peony root, 30g of codonopsis pilosula, 30g of liquorice, 30g of poria cocos and 30g of bighead atractylodes rhizome. Take 3X each time, and take orally. In the formula, radix Codonopsis is matched with radix rehmanniae Preparata, and is sweet in taste, warm in nature, capable of replenishing qi and enriching blood, and is used as a monarch drug. Decoct-quan Dabu Tang (prescription for Tranling and Congratulations) to treat deficiency of both qi and blood. Yellow complexion, lassitude, anorexia, dizziness, short breath, palpitation, spontaneous perspiration, night sweat, cold limbs, pale tongue, thready and weak pulse, metrorrhagia, menoxenia, unhealed sores and ulcers, etc. 6g of codonopsis pilosula, 9g of bighead atractylodes rhizome, 9g of white poria, 9g of white peony root, 12g of astragalus, 6g of ligusticum wallichii, 12g of dry prepared rehmannia root, 9g of angelica, 3g of liquorice, 3 pieces of ginger and 2 jujubes. Take 3X each time, remove the residue and warm it for any time. In the formula, radix Codonopsis is sweet in flavor and warm in property, is used for replenishing qi and enriching blood, and is used as monarch drug. The Chinese book Jing Yuan carries radix codonopsis pilosulae: clearing away the lung-heat; shang Dang ren Shen has the action of clearing lung-heat, though it has no sweet, warm and drastic tonifying action, and is not like Sha Shen which is cold in nature and specially used for purging lung-qi. The formula of the prepared herbal medicine carries radix codonopsitis: adding radix Codonopsis to obtain radix astragali and radix Stephaniae Tetrandrae, mixing with herba Ecliptae for relieving dysentery, adding radix Angelicae sinensis for promoting blood circulation, and adding semen Ziziphi Spinosae for tonifying heart; mixing the lung tonifying honey and steaming; for tonifying spleen and fear, several fractions of sang Pi or Guang Pi can be added. The Chinese herbal medicine Zhengyi carries radix codonopsis pilosulae: the codonopsis pilosula has the effects of tonifying spleen and nourishing stomach, moistening lung and promoting fluid production, and tendons transport qi of middle-jiao, and is not far away from ginseng; especially, the traditional Chinese medicine has the advantages of invigorating spleen, transporting without dryness, nourishing stomach yin without dampness, moistening lung without cold or cool, nourishing blood without greasy feeling, encouraging and clearing yang, vibrating middle-jiao without the defect of dryness; the ginseng has stronger strength than that of Liaoshen, but is less likely to be cloudy and soft, while Korean ginseng has strong smell and slight liability to be hard, and is especially suitable for five zang organs to accept the nutrition, but not suitable for five zang organs; the special strength is weak and can not last for a long time, and people who take two or three money after illness and stop the foot from vibrating their spirit every day are believed to be of moderate and normal scale; because of nourishing Zhongzhou to moisten the four corners, there are not all the ginseng used in ancient and modern prescriptions, i.e. the ginseng used for Bai Zheng, nor the codonopsis pilosula used for Bai Zheng.
Endothelium corneum Gigeriae Galli, named as Chinese medicine, is the inner wall of dry sand sac of Gallusgalussd domestic chickens Brisson of Phasianidae. After killing the chicken, taking out the chicken gizzards, immediately peeling off the inner walls, cleaning and drying. The product is in the form of irregular rolled sheet with thickness of about 2 mm. The surface is yellow, yellow green or yellow brown, is thin and semitransparent, and has obvious stripe wrinkles. It is crisp, brittle, and has cuticular and lustrous appearance. Slightly fishy in smell and slightly bitter in taste. Nature and taste: sweet and neutral. Meridian tropism: it enters spleen, stomach, small intestine and bladder meridians. The efficacy is as follows: strengthening the stomach to promote digestion, arresting seminal emission and relieving enuresis, and treating stranguria and removing calculus. The main treatment is as follows: can be used for treating dyspepsia, emesis, dysentery, infantile malnutrition, enuresis, nocturnal emission, stranguria with stone, pain, gallbladder distention, and hypochondriac pain. In the application aspect, the thirst-quenching drink is one of the following: equally dividing spina bifolium and endothelium corneum Gigeriae Galli into powder; rice is drunk for one money and three days (experience prescription). An incision sore: the effect is immediately achieved when the chicken's gizzard-membrane is burnt to make grey plaster (New book of live children). Malnutrition of vulva: the endothelium corneum Gigeriae Galli (without falling into water) is cleaned, and the new tile is baked to be crisp and becomes fire-toxin, and is pulverized into fine powder. The sore is washed with rice swill and then applied. It is also indicated for aphtha (empirical prescription). Endothelium corneum gigeriae galli carried by Ben Cao gang mu: for removing alcohol accumulation, it is taken with bean powder pill. The Yunnan herbal medicine carries endothelium corneum gigeriae galli: relieving epigastric distention, invigorating spleen, promoting digestion, and grinding stomach; it is indicated for children's food stagnation, abdominal distention, muscular stiffness, and malnutritional stagnation. "Benjing jeep" (original meridian) carries endothelium corneum gigeriae galli: it is indicated for nebula.
The yam is also called yam, dioscorea opposita and dioscorea opposita, which are the herb collected from Chinese materia medica, and the medicinal source is the dried rhizome of dioscorea opposita of the family dioscoreaceae. Rhizoma Dioscoreae has effects of nourishing and strengthening body, promoting digestion, astringing asthenic perspiration, and relieving diarrhea, and can be used for treating spleen deficiency diarrhea, lung deficiency cough, diabetes, frequent micturition, nocturnal emission, female leukorrhagia and chronic enteritis due to dyspepsia. The Chinese yam is most suitable for being matched with the lucid ganoderma for taking, has the function of preventing and treating diabetes, and has great development prospect in the food industry and the processing industry. The yam is sweet in taste and neutral in nature, enters spleen, lung and kidney channels, and has the effects of tonifying spleen and nourishing stomach, promoting fluid production and benefiting lung, and tonifying kidney and arresting seminal emission. It is used for treating spleen deficiency, chronic diarrhea, lung deficiency, kidney deficiency, leukorrhagia, and frequent micturition. Chinese yam, which is eaten by humans from ancient times, is one of the earliest plants eaten by humans. There was a famous sentence for "filled-with-intestines" already in poem of san du pu, down ward poetry. The tuber of the Chinese yam is thick, juicy, sweet, soft and sticky, and is delicious when eaten raw or hot. According to the test result of yam by the Shandong province academy of agricultural sciences, the tuber of the yam contains 14.48 percent of crude protein, 3.48 percent of crude fiber, 43.7 percent of starch, 1.14 percent of sugar, 2.62 percent of potassium, 0.2 percent of phosphorus, 0.2 percent of calcium, 0.14 percent of magnesium, 5.51 percent of ash, 53.57ppm of iron, 29.22ppm of zinc, 10.58ppm of copper and 5.38ppm of manganese on average. The 18 amino acids needed by human beings include 16 Chinese yams. The Chinese yam has the effects of tonifying spleen and stomach and helping digestion, the content of diastase which can decompose starch in the Chinese yam is 3 times that in radish, and the Chinese yam has the function of promoting digestion when being eaten in gastrectasia, can remove uncomfortable symptoms, is beneficial to improving the digestion and absorption functions of spleen and stomach, and is a good product for tonifying the spleen and the stomach; nourishing kidney and replenishing vital essence, the Chinese yam contains mucoprotein, amylase, saponin, free amino acid, polyphenol oxidase and other substances, has rich content and nourishing effect, is a good product for rehabilitation after illness and has the effects of strengthening the body and nourishing kidney and replenishing vital essence; tonifying lung to relieve cough, rhizoma Dioscoreae contains saponin and mucus, has effects of lubricating and moistening, and can be used for invigorating lung qi, nourishing lung yin, and treating cough due to lung deficiency and phlegm; preventing cardiovascular diseases, rhizoma Dioscoreae contains little fat, and mucin can prevent fat deposition in cardiovascular system and prevent arteriosclerosis. The rhizoma Dioscoreae contains saponin, and can reduce cholesterol and triglyceride, and improve hypertension and hyperlipemia; the Chinese yam can increase T lymphocytes of a human body, enhance the immune function and delay cell aging; the Chinese yam has the sedative effect and can resist hepatic coma; the Chinese yam has multiple trace elements, abundant vitamins and mineral substances, particularly high potassium content and relatively low heat, and has the functions of losing weight and building body after being eaten frequently. Modern scientific analysis shows that Chinese yam is characterized by containing a large amount of mucin. Mucin is a mixture of polysaccharide and protein, has special health promotion effect on human body, can prevent fat from depositing on cardiovascular system, maintain blood vessel elasticity, and prevent atherosclerosis from occurring too early; can reduce subcutaneous fat accumulation; can prevent connective tissue atrophy and prevent collagen diseases such as rheumatoid arthritis and scleroderma. Dopamine contained in the composition has important functions of expanding blood vessels and improving blood circulation, and the composition plays an important role in treatment. The Chinese yam has tender meat quality and contains rich nutrient health-care substances. Shen nong Ben Cao Jing is called "mainly strengthen the middle-jiao and tonify deficiency, remove pathogenic cold and heat, tonify middle-jiao and strengthen strength, strengthen muscles, and improve hearing and eyesight after long-term administration"; the Chinese yam has the functions of inducing the generation of interferon and enhancing the immunologic function of human bodies, and the contained choline and lecithin are favorable for improving the memory of people, and the Chinese yam can build up the body and strengthen the body and delay senility after being frequently eaten, is a health-care good product favored by people Stopping lumbago, relieving vexation and fever, invigorating heart-qi deficiency, opening heart orifice, keeping a number of records, invigorating kidney-qi, invigorating spleen and stomach, relieving diarrhea and dysentery, moistening fur, and treating swelling and hard toxin; in Yuye decoction and Zibei decoction in Yi Xue Zhong Can xi Lu, rhizoma Dioscoreae combined with radix astragali can be used for treating diabetes, consumptive disease and dyspnea, and often combined with fructus Lycii, Mori fructus and other medicinal and edible Chinese medicinal materials as tea, and can tonify kidney, strengthen body constitution, enhance resistance, and have good health promotion effect. The Chinese yam has the following functions: the blood pressure lowering Chinese medicine six-ingredient rehmannia bolus, eight-ingredient rehmannia bolus, Guishao rehmannia bolus, etc. are prepared with Chinese yam and are used in treating kidney deficiency, hypertension, diabetes, asthma, neurasthenia, lumbago, etc. 2. Modern science for delaying senescence proves that the yam can obviously reduce the enzymatic activity for accelerating organism senescence; the pill of eight ingredients with rehmannia containing Chinese yam is mainly used for treating postpartum sweating due to debility; baoyuan qingjiang decoction and Baoyuan hanjiang decoction, which can be used for hematemesis and epistaxis; the Hanlin decoction and Ganling decoction can be used for treating stranguria; rhizoma Dioscoreae can also be used for treating pulmonary tuberculosis, typhoid fever and gynecological diseases, which are all beneficial for prolonging life. 3. The Chinese yam tuber with the anti-tumor effect is rich in polysaccharide and can stimulate and regulate the human immune system; therefore, the compound is often used as a health-care medicine for enhancing the immunity; the yam polysaccharide has an antagonistic effect on the cellular immune suppression caused by cyclophosphamide, and can partially or completely restore the suppressed cellular immune function to normal; the Chinese yam can also strengthen the phagocytosis of white blood cells; for example, LIUWEIDIHUANG pill can be used for treating chronic nephritis, hypertension, diabetes, and neurasthenia; the pill can be used for treating ankylosing spondylitis and gynecological vaginal hemorrhage, pudendal pruritus, and amenorrhea; guishao Dihuang Wan can be used for treating otodynia, tinnitus, spontaneous perspiration due to yin deficiency, etc. 4. The allantoin contained in the yam for treating the skin diseases has the effects of anesthesia, analgesia, epithelial growth promotion, inflammation diminishing and bacteriostasis, and is commonly used for treating rhagadia manus et pedis, ichthyosis and various keratoderma diseases.
Astragalus membranaceus, also known as astragalus membranaceus. Perennial herbs with a height of 50-100 cm. The main root is thick, woody, branched and grey-white. The stem is upright, the upper part is multi-branched, and the stem has fine edges and is white and soft. Perennial herbs with a height of 50-100 cm. Produced in inner Mongolia, Shanxi, Gansu, Heilongjiang, etc. The astragalus root has been used for more than 2000 years, and has the functions of enhancing the immunologic function of organisms, protecting the liver, promoting urination, resisting aging, resisting stress, reducing blood pressure and having wider antibacterial action. But excessive superficial pathogenic factors, qi stagnation and dampness obstruction, food stagnation, initial carbuncle and deep-rooted carbuncle or excessive heat-toxicity after ulceration, and yin deficiency and yang hyperactivity are prohibited. Huang Qi is sweet in flavor and slightly warm in nature, and enters lung, spleen, liver and kidney meridians. Radix astragali is rich in calycosin (calycosin) and 3-hydroxy-9, 10-dimethoxy rosewood alkane as flavonoid components, and also contains astragaloside I, V, III (astragaloside I, V, III). The medicinal value of astragalus root mainly includes the following aspects: 1. spontaneous sweating due to exterior deficiency: it is often used for spontaneous sweating due to weakness and weak exterior; for example, if exterior qi is not firm and sweating occurs, Huang Qi combined with Bai Zhu and Fang Feng should be used for treating chronic diseases; as for jade screen powder; it can also be combined with light wheat and radix Ephedrae. 2. Night sweat due to yin deficiency: it is combined with yin-nourishing herbs such as Sheng Di and Mai Dong, etc. 3. Edema due to acute nephritis: it is indicated for edema of deficiency type due to insufficiency of yang qi, and often combined with Fangji, Fu Ling and Bai Zhu, for instance, Fang Ji Huang Tang. 4. Edema due to chronic nephritis, deficiency of spleen and kidney: it is often combined with Dang Shen, Bai Zhu and Fu Ling. 5. Deficiency of yang-qi: it can be used for treating sore and ulcer without rupture and invagination, and has effects of promoting rupture and limiting. For long-time abscess and deep-rooted carbuncle without penetrating head, it is often combined with pangolin scales, Chinese honeylocust spine, Dang Gui and Chuan Xiong. 6. Ulceration of sores: for long-term unhealing, it is usually combined with Yin Hua, Zao Ci and Di Ding, etc., to promote tissue regeneration and heal up. For pus cleansing, it is combined with Dang Shen and Rou Gui. 7. Lung qi deficiency syndrome: cough and dyspnea with a long time, shortness of breath, mental fatigue, phlegm obstructing in the lung and failing to expectorate. It is combined with herbs of warming lung and relieving asthma and strengthening lung qi, such as Zi Wan and Fang Dong, etc. Spleen producing phlegm and lung storing phlegm, so it is good at strengthening taiyin to dispel phlegm, and Huang Qi is good at tonifying qi to treat qi deficiency. 8. Deficiency of vital energy: lassitude, hypodynamia, or sinking of the middle-warmer, rectocele, and prolapse of uterus. For tonifying qi and strengthening spleen, it is often combined with Dang Shen and Bai Zhu; for qi-tonifying and yang-raising with sinking of qi, it is often combined with Dang Shen, Sheng Ma, chai Hu and gan Cao-Zhi. The dietary therapy value of astragalus mainly comprises the following aspects: astragalus membranaceus is a pure natural product which is frequently eaten by people, and people have a smooth running of 'frequently drinking astragalus soup, preventing diseases and protecting health', which means that astragalus membranaceus is frequently used for decocting or soaking in water to replace tea for drinking, and the astragalus membranaceus has good disease prevention and health care effects. Radix astragali and ginseng both belong to good qi-tonifying drugs, and ginseng is mainly used for tonifying primordial qi and restoring yang from collapse, and is commonly used for treating acute diseases such as collapse, shock and the like with good effect. While Huang Qi is mainly tonifying deficiency, it is commonly indicated for chronic weakness, low speech and weak pulse. Some people are easy to catch cold when they meet weather change, the traditional Chinese medicine is called as 'exterior insecurity', astragalus can be used for strengthening exterior, and frequent taking of astragalus can avoid frequent cold. Modern medical research shows that astragalus has the functions of enhancing the immunity of organisms, protecting the liver, promoting urination, resisting aging, resisting stress, reducing blood pressure and resisting a wide range of bacteria. Can eliminate albuminuria caused by experimental nephritis, enhance myocardial contraction force, and regulate blood sugar content. Radix astragali can not only dilate coronary artery, improve myocardial blood supply, and enhance immunity, but also delay cell aging process. The astragalus root is convenient to eat and can be used for decocting soup, decocting paste, soaking wine, adding into dishes and the like.
Pseudo-ginseng, the name of traditional Chinese medicine, is produced mainly in wenshan, Yunnan, so the name wenshan pseudo-ginseng, also the name wenzhou pseudo-ginseng, is the dried root and rhizome of Panax notoginseng Panax notogeng (Burk.) f.h.chen, an araliaceae plant. Digging before blossom in autumn, cleaning, separating main root, branch root and rhizome, and drying. The rootlets are called "ribs" and the rootstocks are called "cut". The main root of pseudo-ginseng is cone-like or cylindrical and 1-6 cm long,the diameter is 1-4 cm. The surface is grayish brown or grayish yellow, with intermittent longitudinal wrinkles and rootage marks. The top has a stem scar and the periphery has a tumor-like protrusion. Heavy, firm, grey-green, yellow-green or grey-white cross section, and the wood parts are arranged slightly radially. Light smell, bitter and sweet taste. The sanchi ribs are cylindrical or conical, the length of the sanchi ribs is 2-6 cm, the diameter of the upper end of the sanchi ribs is about 0.8cm, and the diameter of the lower end of the sanchi ribs is about 0.3 cm. The cut of the pseudo-ginseng is in irregular shrunken blocks or strips, the surface of the cut is provided with a plurality of obvious stem marks and ring lines, the center of the cross section is grayish green or white, and the edge of the cross section is dark green or gray. The notoginseng contains multiple saponins, 9.75-14.90% of the total content, and is similar to the saponins contained in ginseng, but mainly contains dammarane saponin, and ginsenoside Rb1、Rb2、Rc、Rd、Re、Rg1、Rg2、Rh1And notoginsenoside R1～4、R6Etc.; in addition, it also contains heptanine (dencic) and a small amount of flavone as hemostatic active ingredient. Notoginseng is warm in nature, sweet and slightly bitter in taste, and enters liver and stomach meridians. Notoginseng radix has effects of removing blood stasis, stopping bleeding, subsiding swelling and relieving pain, and can be used for treating hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic hemorrhage, thoracico-abdominal pain, and traumatic injury with swelling and pain. The compendium of materia medica carries pseudo-ginseng: stopping bleeding, dissipating blood and relieving pain, and stopping bleeding when the patient suffers from incised wound, traumatic injuries, canker sore and continuous bleeding or is chewed or not mixed; it is also indicated for hematemesis, epistaxis, bloody dysentery, metrorrhagia, profuse menstruation, postpartum lochiorrhea, blood-moving pain, red eye, carbuncle and swelling, and snake bite. The Chinese herbal medicine is called true, and the pseudo-ginseng is loaded: is specially used for liver and stomach; it also enters heart and large intestine. Also known as Chinese lacquer; time is rare; or cloud energy alloy sores; such as paint stickers. The herbal medicine of the new origin carries pseudo-ginseng: dissipating blood and relieving pain; for hematemesis and epistaxis; bloody dysentery with metrorrhagia; blood-shot eyes, abscess and swelling. Carrying radix notoginseng (1765) in compendium of materia Medica, supplement of panax notoginseng: ginseng, radix Ginseng and Notoginseng are the most valuable Chinese herbs because they tonify qi the first and tonify blood the first, and both the flavor and action of Notoginseng are the same. 1912 edition "Chinese medicine dictionary" records: (1) pseudo-ginseng has the functions of enriching blood, removing stasis and bleeding, stopping bleeding and dredging the function, has the best effect and is the most precious of the medicines; pseudo-ginseng is eaten raw, stasis is removed, tissue regeneration is promoted, swelling is reduced, pain is relieved, bleeding is stopped, blood stasis is not left, and new blood is not injured when blood flows; can be taken after being cooked to tonify and strengthen the body; (2) the function of expanding blood vessels; (3) has strong effectRelieving pain, and has effects of relieving fatigue, and improving learning and memory ability; (4) anti-inflammatory effects; (5) has the function of immunomodulator, can restore overhigh or overlow immune response to normal, but does not interfere normal immune response of the organism; (6) anti-tumor effect, inhibiting scar hyperplasia; (7) anti-aging and anti-oxidation effects; (8) reducing blood lipid and cholesterol; (9) protecting liver.
Ramulus Euonymi, also called Euonymus Alata Sieb, herba Bidentis Bipinnatae, herba Rabdosiae Glaucocalycis, rhizoma Farfarae, herba Ricini, herba seu radix Kadsurae Heteroclitae, fructus Caricae, radix Euphorbiae Pekinensis, herba Pileae Scriptae, and herba Ephedrae. Winged branches or winged appendages of euonymus species of the family Celastraceae. Harvesting all the year round, cutting branches, removing twigs and leaves, and drying in the sun; collecting the wing-shaped materials, and drying in the sun. Blood breaking and menstruation promoting; detoxication and detumescence; the efficacy of killing insects. The Euonymus alatus is listed as the middle-quality product in Ben Jing, beginning with the name of Wei Sha Shi. Ceramic hong Jingyun: its stem has three feathers. "compendium" means: between the stones of the ghost arrow, the small plants form clumps, the spring is long and tender, and the four sides of the strip are provided with feathers like arrow feathers, which are regarded as if three feathers. The green leaves are like wild tea, are opposite, and have sour and astringent taste. And thirdly, in April, the flowers are broken and are yellow green. Semen livistonae chinensis is added for fruit setting. "in accordance with its description and with the drawings shall refer to this species. The plant famous entity picture examination income wood class carries: "Wei Sha Yu, that is, ramulus Euonymi. Hunan rustic is called Liuyuelian Ling for swelling and toxicity. The feathered leaves of the ghost arrows contain friedelin alcohol, friedelin, quercetin and dulcitol; the seed oil contains saturated fatty acid (20%), oleic acid, linoleic acid, linolenic acid, caproic acid, acetic acid, benzoic acid, etc.; also contains oxalacetic acid. Ramulus Euonymi is bitter in taste, pungent and cold in nature. The functional indications are as follows: breaking blood and stimulating the menstrual flow; detoxication and detumescence; killing insects; the major symptoms are lumps; pain in the heart and abdomen; amenorrhea; dysmenorrhea; collapse, middle and leakage; postpartum abdominal pain due to stasis; persistent lochia; hernia; arthralgia of the calendar section; sores and swelling; traumatic injury and pain; abdominal pain due to parasitic infestation; scald and burn; bite by poisonous snake. The winged euonymus twig has the function of reducing blood sugar: the sodium oxaloacetate extracted from the euonymus decoction has the effects of reducing blood sugar and urine sugar and increasing weight of normal or alloxan diabetic rabbits; for normal anesthetized dogs, intravenous drip can cause significant blood glucose drop; the oral administration of 5-10 mg/day for 40 days in rats can cause hypoglycemia, proliferation of islet cells, pancreatic beta-cell proliferation and pancreatic alpha-cell atrophy, which indicates that sodium oxaloacetate can stimulate beta-cells, regulate abnormal metabolic process and enhance insulin secretion; the medicine is orally taken by a diabetic patient, and has certain effect when the dosage is 100-1000 mg/day; the Euonymus alatus seeds of the same genus contain cardiac glycoside, which is similar to Convolvulin, but has moderate effect of lowering blood pressure, and can increase coronary artery flow; and can shrink the intestine of guinea pig. The winged euonymus twig has the function of regulating blood fat: the flemingia guttata decoction liquid is 3.6g/(kg.d) and is perfused for 60d, has certain function of regulating blood fat for Japanese quail fed with high cholesterol, can reduce cholesterol and Total Cholesterol (TC) in blood plasma, increase HSL2-c, increase the ratio of HSL2-c/HSL3-c, and increase the acyl-velocity transfer of Lecithin and Cholesterol (LCAT) activity, thereby regulating lipid metabolism and relieving the lesion degree of Atherosclerosis (AS).
Atractylodes Lancea (academic name: Atractylodes Lance a (Thunb.) DC.): atractylodes lancea L.of Compositae is a perennial herb. The rhizome lies horizontally or ascends obliquely, and the root is not fixed. The stem is upright, the height can reach 100 cm, the single stem or a few stems grow into clusters, and the flowering phase of the leaves at the base part falls off; the stem leaves at the middle and lower parts are almost stem-free, round, inverted oval, oblique oval, oval or elliptical, the stem leaves at the upper part or only the upper part are not split, the stem leaves at the middle part are inverted oval, oblong or oblong, all the leaves are hard paper, the two sides are green and hairless, and the edges or the edges of split leaves are provided with needling-shaped hair edges or triangular thorn teeth or heavy thorn teeth. The top of the single stem of the cephalic inflorescence is in a general bract bell shape, and the bract needle is in a pinnate shape and is completely or deeply cracked. White flowers, inverted oval-shaped lean fruits, thick white long straight hair attached in the forward direction, brown or dirty white bristles of crown hair, blossoming and fruiting in 6-10 months. Rhizoma Atractylodis rhizome, as a spleen-activating herb, has the actions of drying dampness, resolving turbidity and alleviating pain. The medicine has many tradenames, such as rhizoma Atractylodis and lance atractylodes rhizome; however, the names of the various products can be roughly divided into two categories, namely north rhizoma atractylodis produced in the north and south rhizoma atractylodis produced in the south.
Rhizoma Atractylodis is pungent and bitter in flavor and warm in nature; it enters spleen, stomach and liver meridians. Rhizoma Atractylodis eliminates dampness and strengthens spleen, dispels wind and cold, improves eyesight and avoids filth. Dampness affecting the spleen and stomach; lassitude for sleeping; distention and fullness in the abdomen; loss of appetite; vomiting and diarrhea; phlegm-fluid retention; edema due to dampness; dampness of exterior syndrome and exterior syndrome; heavy and painful head and body; arthralgia syndrome with warm nature; aching and painful extremities; atrophy of the cutis and the plica; night blindness. Can be used for treating abdominal pain, diarrhea, edema, rheumatalgia, tinea pedis, paralysis 36484m, common cold due to wind-cold evil, and bromhidrosis. In Ben Cao Yan Yi (Yan Yi of materia Medica), cang Zhu is pungent and drastic in flavor. In the pearl sac, cang Zhu is sweet and pungent. Yang and yin. Foot yangming and taiyin. In the recorded of the original source of medical science, cang Zhu is sweet in temperature and flavor. Cang Zhu in Jing Hui Jing Yao (Jing Hui Jing Yao) (rhizoma Atractylodis with bitter and sweet taste) has mild and mild properties. Thick taste and thin smell, yin-yang also. Smelly and nontoxic. Cang Zhu in gang mu is sweet and pungent with warm and dry property, and yin-middle yang can ascend and descend. Enter the channels of foot Taiyin, Yangming, hand Taiyin and Sun. The cang Zhu from Ben Cao Xin Hui (New weaving of materia Medica) enters foot Yangming and Taiyang meridians. The cang Zhu enters spleen and liver meridians in Ben Cao Xin (materia Medica regeneration).
Radix scrophulariae (Scrophyllum nivensis Hemsl.) is a herbal plant of Scrophulariaceae, and can reach more than 1 meter. The branch root is several, the spindle shape or carrot shape is expanded, the thickness can reach more than 3 cm. It grows in bamboo groves, creeks, jungles and tall grass jungles with the elevation below 1700 m. Hebei (southern), Henan, Shanxi, Shaanxi (southern), Hubei, Anhui, Jiangsu, Zhejiang, Fujian, Jiangxi, Hunan, Guangdong, Guizhou and Sichuan. Radix scrophulariae has effects of clearing heat and cooling blood, nourishing yin and lowering fire, and removing toxic substance and resolving hard mass. Heat in disease and nutrient-blood are mainly warmed; fever; polydipsia; a deep-red tongue; carrying out spot formation; bone steaming, strain cough; restlessness due to deficiency in the middle energizer; constipation due to body fluid impairment; eyes are astringent and dim; swollen and sore throat; scrofula and subcutaneous nodule; abscess, deep-rooted carbuncle and sore. Radix scrophulariae mainly contains iridoids, phenylpropanoid glycosides, phytosterol, organic acids, flavonoids, triterpenoid saponin, volatile oil, saccharides, alkaloids, trace monoterpene and diterpene components and the like. The iridoid component of figwort is classified into 4 types: cyclopentane type, 7, 8-cyclopentene type and 7, 8-epoxycyclopentane type of nine-carbon skeleton and variant iridoids. About phenylpropanoid glycosides, 11 phenylpropanoid glycoside compounds are separated from figwort root: astroceroside A (i.e. 6-O-trans-cinnamoyl-1-O-alpha-D-fructosyl-8-D-glucose, sibirioside A, XS-4), Staphylin F (i.e. 4-O-caffeoyl-3-O-alpha-L-rhamnosyl-D-glucose, cistanoside F, XS-5), angoroside C (XS-8), Staphylin D (cistanoside D, XS-9), verbascoside (acteoside, XS-10), decaffeoylverbascoside (decaffeoylacteoside, XS-11), 3-O-acetyl-2-O-feruloyl-alpha-L-rhamnose (ninoposide A, XS-12), 4-O-acetyl-2-O-acetyl-alpha-L-rhamnose- Rhamnose (ning poside B, XS-13), 3-O-acetyl-2-O-p-hydroxycinnamoyl- α -L-rhamnose (ning poside C, XS-14), 4-O- (p-methoxycinnamoyl) - α -L-rhamnose and 3-O-acetyl-2-O-p-methoxycinnamoyl- α -L-rhamnose (ning poside D). In addition, radix scrophulariae also contains sterol and its glycosides such as G-sitosterol, daucosterol, etc. In addition, radix scrophulariae also contains organic acids such as cinnamic acid, 4-hydroxy-3-methoxybenzoic acid, ferulic acid, p-methoxycinnamic acid, and succinic acid; 5-hydroxymethylfurfural; diterpene cryptomerin alcohol; triterpenes ursolic acid, asparagine, fructose, sucrose, glucose, triterpenoid saponin, flavonoid aglycone, alkaloid, volatile oil, etc. In the aspect of pharmacological action of figwort, 1, influence on cardiovascular system: (1) coronary artery dilation: the figwort alcohol extract aqueous solution can obviously increase the coronary flow of isolated rabbit heart, and has slight inhibition on heart rate and heart contractility; radix scrophulariae can significantly increase myocardial nutritional blood flow of mice, and has significant effect on resisting coronary contraction caused by hypophysin of mice. (2) The function of reducing blood pressure: pharmacological research shows that the figwort water extract, the figwort alcohol extract and the decoction all have the function of reducing blood pressure. Intravenous injection of radix scrophulariae alcoholic extract can lower blood pressure of anesthetized cat by 40.5% on average; the decoction has more obvious effect of reducing blood pressure of the renal hypertension dogs. The initial analysis of the antihypertensive effect shows that the figwort has no effect on the alpha-adrenergic receptor and has no obvious influence on the pressure-increasing reflex caused by blocking the carotid blood flow, and the antihypertensive mechanism may be related to the vasodilatation and needs to be further researched. (3) Anti-platelet aggregation effect: phenylpropanoid glycosides XS-8, XS-10 and iridoid glycosides XS-6, XS-7 have anti-platelet aggregation effect at 0.5mmol/L, but the phenylpropanoid glycosides have strong effect. Phenylpropanoid glycoside XS-8 has a reducing effect on both TXB2 and 6-keto-PGF10 in plasma, but has a more obvious reducing effect on TXE2, and is probably a mechanism for resisting platelet aggregation. The lipophilic component of radix scrophulariae also has the effect of inhibiting platelet aggregation, and the inhibition rates of radix scrophulariae ether extraction, alcohol extract and water extract are respectively 55.5%, 40.5% and 51.9% under the same dosage condition, and the three components all have the effect of remarkably reducing the platelet aggregation rate. (4) Fibrinolysis promoting effect: the radix scrophulariae ether, alcohol and water extracts have obvious effect of reducing PAI-1, wherein the radix scrophulariae petroleum ether extract has the strongest effect, and has obvious difference compared with a normal saline control group and a salvia miltiorrhiza water extract control group with the same dose, and also has obvious difference compared with an aspirin group. (5) Improving the rheological property of blood: the figwort ether, alcohol and water extract have no obvious influence on the whole blood viscosity, the whole blood reduction viscosity, the plasma viscosity and the hematocrit of a rat, but the figwort extract can obviously improve the cortex CBF (P percent 0.05) after 2 hours of ischemia, wherein a 5mg/kg dose group has obvious effect on the blood flow improvement (P <0.05) at each ischemia time point. (6) The effect of resisting cerebral ischemia injury is as follows: the radix scrophulariae extract has a protective effect on cerebral ischemia of rats, cerebral infarction volume of rats 24h after ischemia can be obviously reduced by tail vein injection of the radix scrophulariae extract, nerve functions are obviously improved, and the effect may be related to improvement of cerebral blood flow. 2. The analgesic effect is as follows: the figwort oral liquid has obvious inhibition effect on mouse writhing reaction caused by acetic acid after being taken for 1 hour, and the effect and the dosage have certain dependence relationship. 3. Anti-inflammatory action: radix scrophulariae has anti-inflammatory effect, and can be used for treating gingivitis, tonsillitis, pharyngolaryngitis, etc. Radix scrophulariae has obvious inhibiting effect on mouse concha swelling caused by inflammation of croton oil, rat toe swelling caused by induction of egg white, carrageenan and cobra venom, and mouse granuloma formation. 4. The antibacterial effect is as follows: the radix scrophulariae and folium Ginseng have weak bactericidal effect, and the minimum bactericidal concentration is 50mg or more per 1 ml. The XUANSHENYE has stronger antibacterial effect than root, especially on Staphylococcus aureus, and has inferior effect on diphtheria, typhoid bacillus, and beta-streptococcus, but is weaker than rhizoma Coptidis. 5. Immunopotentiating activity: the hypodermal injection of harpagoside can restore the immune function inhibited by yin deficiency mice; the harpagide and harpagoside can promote the proliferation of splenic lymphocytes in mice with yin deficiency. Both radix scrophulariae and radix astragali can increase leukocyte count and thymus index under physiological conditions and the condition of cyclophosphamide induced immune function inhibition. 6. Liver protection: phenylpropanoid glycoside in radix scrophulariae has liver protecting effect. Researches find that the phenylpropanoid glycoside XS-10 has obvious protective effect on hepatic cell injury caused by D-galactosamine and can inhibit the hepatic cell apoptosis. The anti-liver injury apoptosis may be related to the regulation of hepatocyte apoptosis related genes. 7. Antioxidation: the antioxidant activity of phenylpropanoid glycosides in radix scrophulariae is significantly stronger than that of iridoids. Phenylpropanoid glycosides XS-8 and XS-10 exert significant repairing effects on deoxynucleotide hydroxyl addition free radicals, while iridoid glycosides XS-6 and XS-7 do not exert significant effects under the same conditions. Has obvious inhibiting effect on four kinds of red blood cell oxidative hemolysis, and the former two are obviously stronger than the latter two.
Rehmanniae radix, REHMANNIAE RADIX, which is fresh or dried root tuber of rehmannia glutinosa libosch of Scrophulariaceae family. Collected in autumn, removed rhizoma Phragmitis, fibrous root and silt, and slowly baked radix rehmanniae to about eighty percent dry, which is the radix rehmanniae. Rehmannia belongs to perennial herbaceous plants, the height can reach 30 cm, the rhizome is fleshy, the rehmannia is fresh yellow, and the stem is purple red under the cultivation condition. The diameter can reach 5.5 cm, the leaves are oval to oblong, the veins are sunken on the upper surface, flowers are slightly arranged into a general inflorescence at the top of the stem, the corolla is purplish red, the interior is yellowish purple, the medicine chamber is rectangular and circular, the capsule is oval to oblong, and the flowering period is 4-7 months. Fresh rehmannia root is sweet, bitter and cold in nature; dried rehmannia root is sweet in taste and cold in nature; prepared rehmannia root, radix rehmanniae Praeparata is sweet in taste and slightly warm in nature. Rehmannia enters heart, liver and kidney meridians. Rehmannia is cool in nature and sweet and bitter in taste, and has the effects of nourishing yin, tonifying kidney, nourishing blood, enriching blood and cooling blood. It is beneficial to be taken by patients with yin deficiency, blood deficiency and kidney deficiency. In addition, rehmanniae radix has effects of tonifying heart, promoting diuresis, relieving fever, relieving inflammation, promoting blood coagulation and reducing blood sugar. Fresh rehmannia root: clear heat and promote fluid production, cool blood and stop bleeding. Can be used for treating yin impairment due to fever, crimson tongue, polydipsia, macula, eruption, hematemesis, epistaxis, and sore throat. Dried rehmannia root: clear heat and cool blood, nourish yin, promote the production of body fluid. Can be used for treating fever with deep-red tongue, polydipsia, yin deficiency, internal heat, bone steaming, internal heat, diabetes, hematemesis, epistaxis, macula, and eruption. Prepared rehmannia root: to nourish yin, enrich blood, replenish essence and replenish marrow. Can be used for treating deficiency of liver-yin and kidney-yin, soreness of waist and knees, hectic fever, night sweat, nocturnal emission, internal heat, diabetes, blood deficiency, sallow complexion, cardiopalmus, severe palpitation, menoxenia, metrorrhagia, metrostaxis, hemorrhage, giddiness, tinnitus, and early white beard and hair. The chemical components of rehmanniae radix are mainly glycosides, and iridoid glycosides are mainly contained therein. Iridoid glycosides obtained from fresh rehmannia glutinosa have the following properties: leonuride (leonuride), aucubin (aucubin), catalpol (catalpol), rehmanniside (rehmanniside) A, B, C, D, meilitoside (melittoside), victoride (melittoside), dianiside (melittioside), 8-epilogeninic acid (8-epiloganic acid), boat ostearic acid (ajugaside), 6-O-E-feruloyl ajugal (6-O-E-feruloyl ajugal), 6-O-Z-feruloyl ajugal (6-O-Z-feruloyl 6L ajugal), 6-O-vanillyl ajugal (6-O-vanillylo ajugal), 6-O-p-coumaroyl ajugal (6-O-p-coumaroyl ajugal), 6-O- (4 '-O-alpha-pyrane L) (6-O-4' -cumaroyl ajugal) (4-rhamnogalacturonol- α -L-rhamnopyranosyl) vanillonyl ajugol ], digitonin (genioglutaside) A, B, etc.; the content of catalpol is highest. And also contains saccharides: d-glucose (D-glucose), D-galactose (D-galactose), D-fructose (D-fructose), sucrose (sucrose), raffinose (raffinose), stachyose (stachyose), mannotriose (amn-ninotcMLIBiose), verbascose (verbascose) in the amount of up to 64.9% of stachyose. And further contains lysine (lysine), histidine (histidine), arginine (arginin), aspartic acid (aspartic acid), glutamic acid (glutamic acid), threonine (threonine), serine (serine), glycine (glycine), alanine (alanine), valine (valine), isoleucine (isoeucine), leucine (leucine), tyrosine (tyrosine), phenylalanine (phenylalanine), gamma-aminobutyric acid (gamma-aminobutyric acid), and the like amino acids, and glucose (glucosamine), D-glycoside dew alcohol (D-mannitol), phosphoric acid (phosphonic acid), beta-sitosterol (beta-sitosterol), daucosterol (1-ethyl-beta-D-glucosamine), adenosine (adenosine), inorganic elements, and the like. The root contains rehmanniae radix glycoside A, B, C, D, dihydrocatalpol glycoside (dihydrocatalpol), aucubin (aucubin), catalpol glycoside (catalpol), melittoside and leonuride. The fresh root contains catalpol glycoside 0.11%. The fresh root alcohol extract also contains beta-sitosterol, mannitol, carosterol (daucosterol), 1-ethyl-beta-D-galactoside and sucrose. The water soluble component contains several kinds of sugar, including stachyose with the highest content of 32.1-48.3%, amino acids with the highest content of arginine of 2-4.2%, amino butyric acid and phosphoric acid. The rehmannia root product has small amount of beta-sitosterol, stigmasterol and campesterol. The dried rehmannia root is divided into fatty acid, beta-sitosterol, palmitic acid, succinic acid, daucosterol and S8 cyclic compound. The aerial part contains monomelitoside, leonuride, aucubin, eukoside, and dichloroside. The pharmacological action of rehmannia mainly comprises the following aspects: the effective part (R-BP-F) of the Huaiqing rehmannia is injected in the abdominal cavity, and has the function of reducing blood sugar of mice experimental diabetes caused by alloxan. The hemostasis is achieved: the blood coagulation time of mice can be shortened by intragastric administration of radix rehmanniae, radix rehmanniae Preparata decoction, radix rehmanniae preparata and radix rehmanniae Preparata preparata (capillary tube method). Resistance to disseminated intravascular coagulation: the rehmanniae radix 70% methanol extract can inhibit platelet aggregation of rat caused by ADP, has antithrombin effect, and can resist diffuse intravascular coagulation of rat caused by endotoxin. In addition, it also has anti-inflammatory, immunity enhancing, and liver injury resisting effects. Fourth, hepatitis treatment: in clinical reports, the combination of rehmannia root and liquorice has a certain effect on infectious hepatitis whether intramuscular injection or oral administration, promotes the recovery of liver function, and particularly has obvious ALT reduction and no local and systemic adverse reactions. Fifthly, treating diphtheria: takes radix rehmanniae as the main material and is matched with diphtheria resistant mixture of forsythia, scutellaria, ophiopogon root and figwort, and the fever can be abated within 4 days after the taking, the false membrane disappears and the sore throat is improved.
The invention can obtain the excellent biological effect by preparing fourteen medicines such as the codonopsis pilosula into a preparation form for external application and pasting the preparation form on three acupuncture points.
Detailed Description
The invention is further illustrated by the following specific examples/experimental examples, but it should be understood that these examples and experimental examples are intended only for the purpose of more detailed description and are not intended to limit the invention in any way.
The present invention has been described generally and/or specifically with respect to materials used in testing and testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible. It will be apparent to those skilled in the art that the materials and methods of operation used in the present invention are well known in the art, unless otherwise specified.
Preparation example 1: preparation of a Chinese medicinal composition
The formula is as follows: 18 parts of codonopsis pilosula, 23 parts of astragalus membranaceus, 23 parts of radix scrophulariae, 23 parts of dendrobe, 18 parts of radix ophiopogonis, 23 parts of radix rehmanniae recen, 18 parts of stiff silkworm, 23 parts of coptis chinensis, 23 parts of pseudo-ginseng, 18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis, 23 parts of winged euonymus twig, 30 percent of vaseline (by total weight of the medicinal powder) and 3 percent of azone (by total weight of the medicinal powder.
The preparation method comprises the following steps:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving the mixture by a 150-mesh sieve, adding the mixed fine powder into a mixed solution of vaseline and azone preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain a Chinese medicinal composition in the form of ointment; optionally, the step of (a) is carried out,
(4) and (3) coating the soft paste traditional Chinese medicine composition obtained in the step (3) on non-woven fabrics, and covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum (each plaster equivalently contains 0.15 g or 0.3g of codonopsis pilosula, which can be called blood sugar reduction moxibustion or triple-jiao blood sugar reduction moxibustion, and of course, can contain larger doses of medicines when needed).
Because the traditional Chinese medicines of the compositions of preparation examples 1-7 are directly crushed into preparations without extraction, the obtained fine powder can pass through a 100-mesh sieve or even a smaller sieve, such as 150-mesh sieve, and has important significance for ensuring the biological effect of the composition.
In preparation examples 1-7 of the invention, radix ophiopogonis, pseudo-ginseng, Chinese yam and endothelium corneum gigeriae galli are crushed together in the preparation step (1), fine powder which can pass through a 150-mesh sieve can be successfully obtained, and the sieve does not adhere to the sieve and does not block the sieve pores; the fine powder which can pass through a 150-mesh sieve can be obtained by crushing in the preparation step (2), and the sieve can not be bonded and can not block the sieve; the two kinds of powder in the preparation step (3) can be mixed smoothly and can pass through a 150-mesh sieve without generating the phenomena of viscous substances and blocking the sieve. In a supplementary test, referring to the formulas and the preparation methods of preparation examples 1 to 7, the inventor of the present invention found that when yam was pulverized alone, or yam was pulverized together with the other 13 kinds of medicines, or yam was pulverized together with ophiopogon root, or yam was pulverized together with panax notoginseng, or yam was pulverized together with endothelium corneum gigeriae galli, fine powder that could pass through a sieve of 80 mesh was not obtained, and a sieve of 100 mesh or 150 mesh was not obtained, and each material generated sticky substances and clogged the sieve during the pulverization, which was completely unacceptable.
Test example 1: therapeutic Effect test of the composition of the present invention-Observation of clinical efficacy of the composition for diabetes
1. General data
The number of patients with type 2 diabetes mellitus who are diagnosed in hospitals and are orally administered with metformin hydrochloride according to the prescription of doctors to control blood sugar is 180 in total, the patients are 52-67 years old, 58.3 years old on average, 96 men and 84 women, all the patients control diet according to the medical advice before being included in the test and can satisfactorily control blood sugar before the test, but all the patients suffer from one or more adverse reactions of diarrhea, nausea, vomiting, gastrectasia, hypodynamia, dyspepsia and abdominal discomfort, and all the patients maintain the diet controlled at ordinary times after entering the test.
2. Grouping and administration of drugs
Patients are divided into three groups at random, each group comprises 32 men and 28 women, group A continues to take metformin hydrochloride orally according to the prescription of a doctor, group B reduces the dosage of metformin hydrochloride by half and sticks to the codonopsis pilosula 0.15 g of the preparation example 1 for 1 time every day, group C interrupts metformin hydrochloride and sticks to the codonopsis pilosula 0.3g of the preparation example 1 for 2 times every day. The test was conducted for 30 days, and blood glucose was measured and gastrointestinal reactions were observed daily, and the blood glucose control status and the incidence of gastrointestinal reactions of all patients within 10 days before the test were counted on days 10, 20 and 30.
The plaster method of blood sugar-reducing moxibustion comprises the following steps: sterilizing Shenque acupoint, Xuehai acupoint and Xiaoke acupoint with 75% alcohol, applying blood sugar lowering moxibustion, removing after 9 hr (applying blood sugar lowering moxibustion once per day for 8 hr, removing after the same time the next day, applying blood sugar lowering moxibustion twice per day for 8 hr, removing after the rest for 4 hr, and rest for 4 hr).
3. Results
The score of the blood sugar control condition is calculated by 10 points, the higher the score is, the better the control is, the 10 points are completely controlled, and the 0 point is not effectively controlled; the gastrointestinal tract reaction rate is calculated by 100%, and the higher the percentage is, the higher the gastrointestinal tract reaction rate is, and the range is 0-100%. The glycemic control status and incidence of gastrointestinal response for each group were expressed as the mean of all patient scores and the results were as follows: the 10 th-day blood sugar control scores of the group A, the group B and the group C are respectively 9.7, 7.8 and 3.7, and the gastrointestinal tract reaction incidence scores are respectively 96.3%, 64.7% and 13.4%; the 20 th day blood sugar control scores of the group A, the group B and the group C are respectively 9.4, 8.9 and 5.9, and the gastrointestinal tract reaction incidence scores are respectively 94.7%, 20.5% and 1.6%; the 30 th-day blood sugar control scores of the group A, the group B and the group C are respectively 9.8, 9.3 and 8.3, and the gastrointestinal tract reaction incidence scores are respectively 97.8%, 14.7% and 0.
Of the above results, 60 patients in each group had a mean or percentage of 600 observed values over a 10 day observation period. The results show that group a patients who continued to take metformin still maintained good glycemic control, while gastrointestinal reactions were reported with an incidence of close to 100%; the B group which reduces the dosage of the metformin and gives the blood sugar-reducing moxibustion has obviously reduced blood sugar control effect and reduced adverse reaction incidence rate at the initial stage, and the blood sugar control effect is gradually improved and the adverse reaction incidence rate is gradually reduced to about 15 percent along with the medication; after metformin is taken intermittently, the blood sugar control of group C subjected to blood sugar reduction moxibustion is remarkably reduced and the adverse reaction is reduced to below 15% in the first 10 days, and then the adverse reaction is gradually reduced to 0 and the blood sugar control is gradually improved along with the medication until the third 10 days is more than 8 minutes. The results show that the composition has excellent effect of controlling blood sugar, and has no adverse reaction, particularly no gastrointestinal adverse reaction; according to the results of group B, the compositions of the present invention even have the effect of reducing the incidence of adverse reactions to metformin. In addition, in some supplementary experiments of the present inventors, referring to a composition prepared by the formulation and preparation method of preparation example 1, which is different from the composition prepared by the formulation and preparation method only without Bombyx Batryticatus, it was found that such a composition without Bombyx Batryticatus could not effectively control blood glucose when tested by the formulation method of B, C described above, for example, the blood glucose control scores on the 10 th day, the 20 th day and the 30 th day were 2.3, 0.2 and 0.1, respectively, when referring to group C, indicating that the composition of the present invention could not achieve hypoglycemic effect without Bombyx Batryticatus.
Test example 2: quality testing of the compositions of the invention
In the traditional Chinese medicine composition, the codonopsis pilosula is used as a monarch drug to play a therapeutic role, and as a drug expected to be used in clinic, the quality control of the traditional Chinese medicine composition is required, for example, the identification of the existence of the codonopsis pilosula in the composition is an extremely important quality detection item. Under the item of radix codonopsitis recorded on 281 page of the first edition of Chinese pharmacopoeia 2015, a method for identifying radix codonopsitis by using thin-layer chromatography is provided, and the method in the pharmacopoeia can be used for identifying whether the traditional Chinese medicinal materials are radix codonopsitis or not. In the present invention, it is necessary to identify whether the composition contains codonopsis pilosula, and if codonopsis pilosula is not added as a monarch drug, the biological effect of the composition may be seriously affected. The inventor of the present invention uses the pharmacopeia method to identify whether the codonopsis pilosula is added to the traditional Chinese medicine composition obtained by the present invention, and has surprisingly found that the existence of the codonopsis pilosula can be identified by improving the pharmacopeia method probably due to the existence of azone in the composition, otherwise, the codonopsis pilosula cannot be detected.
In this test example 2, the TLC method is used to identify whether codonopsis pilosula is added to the composition, which includes the following steps:
(a) taking a traditional Chinese medicine composition which is equivalent to 1g of codonopsis pilosula marked by 1g, adding 25ml of methanol, 5ml of ethyl acetate and 1ml of formic acid, carrying out ultrasonic treatment for 30 minutes, filtering, evaporating filtrate to dryness, adding 15ml of water into residue to dissolve the residue, passing the residue through a D101 type macroporous adsorption resin column (the inner diameter is 1.5cm, the column height is 10cm), eluting with 50ml of water, discarding water solution, eluting with 50ml of 50% ethanol, collecting eluent, evaporating to dryness, adding 1ml of methanol into residue to dissolve the residue to obtain a sample solution;
(b) adding methanol into radix Codonopsis alkyne glycoside reference substance to obtain 1mg solution per 1ml as reference substance solution;
(c) performing thin-layer chromatography (0502 of general rules of the four parts of the national pharmacopoeia 2015), sucking 2-4 ul of a sample solution and 2ul of a reference solution, respectively dropping on the same high-efficiency silica gel G thin-layer plate, developing with n-butyl alcohol-glacial acetic acid-water (7: 1: 0.5) as a developing agent, taking out, air drying, spraying with 10% sulfuric acid ethanol solution, heating at 100 ℃ until the spots are clearly developed, and respectively inspecting under sunlight and an ultraviolet lamp (365 nm);
(d) the composition added with radix Codonopsis in the chromatogram of the test sample shows spots or highlight spots with the same color at the corresponding positions of the chromatogram of the control sample; if no corresponding spot is shown, it indicates that no radix Codonopsis has been added to the composition. The TLC method is used for detecting the traditional Chinese medicine composition obtained in the preparation examples 1-7, the existence of the codonopsis pilosula can be detected, and the spot size and the color depth of a sample to be detected are basically the same as those of a codonopsis pilosula medicinal material which is equivalent to the theoretical codonopsis pilosula in the composition, so that the codonopsis pilosula can be detected by the method, and the amount of the detected codonopsis pilosula is consistent with the theoretical feeding amount.
In a supplementary test of the present invention, referring to the TLC method of test example 2 above, except that neither ethyl acetate nor formic acid was added in step (a), the compositions obtained in preparation examples 1 to 7 were tested, and the results showed that the presence of Codonopsis pilosula was not detected, and no spots were shown on the thin layer plate of the test article at the position of Codonopsis tangshen alkynoside. In a supplementary test of the present invention, the compositions obtained in preparation examples 1 to 7 were tested with reference to the TLC method of test example 2 described above, except that formic acid was not added in step (a), and the results showed that the presence of Codonopsis pilosula was not detected, and no spots were shown on the Codonopsis pilosula alkynoside on the thin layer plate of the test article. In a supplementary test of the present invention, the compositions obtained in preparation examples 1 to 7 were tested with reference to the TLC method of test example 2 described above, except that ethyl acetate was not added in step (a), and the results showed that the presence of Codonopsis pilosula was not detected and no spots were shown on the thin layer plates of the test articles. In a supplementary test of the present invention, referring to the formulations and the preparation methods of preparation examples 1 to 7, except that azone was not added, a composition was obtained; the TLC method of test example 2 was used to determine the compositions without azone, and the results showed that the presence of Codonopsis pilosula was detected, and the same spots were observed on the tested thin layer plates. In a supplementary test of the present invention, referring to the formulations and the preparation methods of preparation examples 1 to 7, except that azone was not added, a composition was obtained; referring to the TLC method of test example 2 above, except that neither ethyl acetate nor formic acid was added in step (a), these compositions without azone were tested and showed the presence of Codonopsis pilosula was detected, and the same spots were shown on the thin layer plates of the test article at the position of Codonopsis pilosula alkynol.
Preparation example 2: preparation of a Chinese medicinal composition
The formula is as follows: 15 parts of codonopsis pilosula, 25 parts of astragalus membranaceus, 20 parts of radix scrophulariae, 25 parts of dendrobe, 15 parts of radix ophiopogonis, 25 parts of radix rehmanniae recen, 15 parts of stiff silkworm, 25 parts of coptis chinensis, 20 parts of pseudo-ginseng, 20 parts of Chinese yam, 15 parts of endothelium corneum gigeriae galli, 20 parts of cinnamon, 15 parts of rhizoma atractylodis, 25 parts of winged euonymus twig, 25 percent of vaseline (by total weight of the medicinal powder) and 4 percent of azone (by total weight of the medicinal powder.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
Preparation example 3: preparation of a Chinese medicinal composition
The formula is as follows: 20 parts of codonopsis pilosula, 20 parts of astragalus membranaceus, 25 parts of radix scrophulariae, 20 parts of dendrobe, 20 parts of radix ophiopogonis, 20 parts of radix rehmanniae recen, 20 parts of stiff silkworm, 20 parts of coptis chinensis, 25 parts of pseudo-ginseng, 15 parts of Chinese yam, 20 parts of endothelium corneum gigeriae galli, 15 parts of cinnamon, 20 parts of rhizoma atractylodis, 20 parts of winged euonymus twig, 35 percent of vaseline (calculated on the total weight of the medicinal powder) and 2 percent of azone (calculated on the total weight.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
Preparation example 4: preparation of a Chinese medicinal composition
The formula is as follows: 25 parts of codonopsis pilosula, 20 parts of astragalus membranaceus, 25 parts of radix scrophulariae, 20 parts of dendrobe, 25 parts of radix ophiopogonis, 20 parts of radix rehmanniae recen, 25 parts of stiff silkworm, 20 parts of coptis chinensis, 25 parts of pseudo-ginseng, 25 parts of Chinese yam, 10 parts of endothelium corneum gigeriae galli, 25 parts of cinnamon, 15 parts of rhizoma atractylodis, 25 parts of winged euonymus twig, 40% of vaseline (by total weight of the medicinal powder) and 1% of azone (by total weight of the medicinal powder.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
Preparation example 5: preparation of a Chinese medicinal composition
The formula is as follows: 10 parts of codonopsis pilosula, 25 parts of astragalus membranaceus, 20 parts of radix scrophulariae, 25 parts of dendrobe, 15 parts of radix ophiopogonis, 25 parts of radix rehmanniae recen, 10 parts of stiff silkworm, 25 parts of coptis chinensis, 20 parts of pseudo-ginseng, 10 parts of Chinese yam, 25 parts of endothelium corneum gigeriae galli, 10 parts of cinnamon, 25 parts of rhizoma atractylodis, 20 parts of winged euonymus twig, 20 percent of vaseline (by total weight of the medicinal powder) and 5 percent of azone (by total weight of the medicinal powder.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
Preparation example 6: preparation of a Chinese medicinal composition
The formula is as follows: 30 parts of codonopsis pilosula, 15 parts of astragalus membranaceus, 30 parts of radix scrophulariae, 15 parts of dendrobe, 30 parts of radix ophiopogonis, 15 parts of radix rehmanniae recen, 30 parts of stiff silkworm, 20 parts of coptis chinensis, 30 parts of pseudo-ginseng, 5 parts of Chinese yam, 30 parts of endothelium corneum gigeriae galli, 5 parts of cinnamon, 30 parts of rhizoma atractylodis, 15 parts of winged euonymus twig, 35 percent of vaseline (calculated by the total weight of the medicinal powder) and 2 percent of azone (calculated by the total weight.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
Preparation example 7: preparation of a Chinese medicinal composition
The formula is as follows: 5 parts of codonopsis pilosula, 30 parts of astragalus membranaceus, 15 parts of radix scrophulariae, 30 parts of dendrobe, 10 parts of radix ophiopogonis, 30 parts of radix rehmanniae recen, 5 parts of stiff silkworm, 30 parts of coptis chinensis, 15 parts of pseudo-ginseng, 30 parts of Chinese yam, 5 parts of endothelium corneum gigeriae galli, 30 parts of cinnamon, 10 parts of rhizoma atractylodis, 30 parts of winged euonymus twig, 25 percent of vaseline (by total weight of the medicinal powder) and 4 percent of azone (by total weight of the medicinal powder.
The preparation method comprises the following steps: the procedure was as described in preparation example 1.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims (8)

1. A method for detecting a traditional Chinese medicine composition for treating diabetes mellitus is provided, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines: 5-30 parts of codonopsis pilosula, 15-30 parts of astragalus membranaceus, 15-30 parts of radix scrophulariae, 15-30 parts of dendrobe, 10-30 parts of radix ophiopogonis, 15-30 parts of radix rehmanniae recen, 5-30 parts of stiff silkworm, 20-30 parts of coptis chinensis, 15-30 parts of pseudo-ginseng, 5-30 parts of Chinese yam, 5-30 parts of endothelium corneum gigeriae galli, 5-30 parts of cinnamon, 10-30 parts of rhizoma atractylodis and 15-30 parts of winged euonymus twig, and the traditional Chinese medicine composition is prepared by the following steps:
(1) pulverizing radix Ophiopogonis, Notoginseng radix, rhizoma Dioscoreae and endothelium corneum Gigeriae Galli into 150 mesh fine powder;
(2) pulverizing the rest ten Chinese medicinal materials into 150 mesh fine powder;
(3) uniformly mixing the fine powder obtained in the step (1) and the step (2), sieving the mixture by a 150-mesh sieve, adding the mixed fine powder into a mixed solution of vaseline and azone preheated to 55-60 ℃ under stirring, and uniformly stirring to obtain a Chinese medicinal composition in the form of ointment; wherein the weight ratio of the medicinal powder to the vaseline is 100: 20-40, wherein the weight ratio of the medicinal powder to azone is 100: 1-5;
(4) coating the pasty traditional Chinese medicine composition obtained in the step (3) on non-woven fabrics, covering an isolating membrane to obtain the traditional Chinese medicine composition in the form of emplastrum,
the method comprises the following steps of identifying the codonopsis pilosula in the composition:
(a) taking a traditional Chinese medicine composition which is equivalent to 1g of codonopsis pilosula marked by 1g, adding 25ml of methanol, 5ml of ethyl acetate and 1ml of formic acid, carrying out ultrasonic treatment for 30 minutes, filtering, evaporating filtrate to dryness, adding 15ml of water into residue to dissolve the residue, passing the residue through a D101 type macroporous adsorption resin column with the inner diameter of 1.5cm and the column height of 10cm, eluting with 50ml of water, discarding water solution, eluting with 50ml of 50% ethanol, collecting eluent, evaporating to dryness, and adding 1ml of methanol into the residue to dissolve the residue to obtain a sample solution;
(b) adding methanol into radix Codonopsis alkyne glycoside reference substance to obtain 1mg solution per 1ml as reference substance solution;
(c) according to a thin-layer chromatography test recorded by the general rule 0502 of the four parts of the national pharmacopoeia 2015 year edition, sucking 2-4 mu l of a sample solution and 2 mu l of a reference substance solution, respectively dropping the samples on the same high-efficiency silica gel G thin-layer plate, and measuring the samples in a volume ratio of 7: 1: developing with 0.5 n-butanol-glacial acetic acid-water mixed solution as developing agent, taking out, air drying, spraying 10% sulphuric acid ethanol solution, heating at 100 deg.C until spots are clearly developed, and inspecting under sunlight and 365nm ultraviolet lamp respectively;
(d) the composition added with radix Codonopsis in the chromatogram of the test sample shows spots or highlight spots with the same color at the corresponding positions of the chromatogram of the control sample; if no corresponding spot is shown, it indicates that no radix Codonopsis has been added to the composition.
2. The method according to claim 1, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight: 10-25 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-25 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 10-25 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 10-25 parts of Chinese yam, 10-25 parts of endothelium corneum gigeriae galli, 10-25 parts of cinnamon, 15-25 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
3. The method according to claim 1, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight: 15-20 parts of codonopsis pilosula, 20-25 parts of astragalus membranaceus, 20-25 parts of radix scrophulariae, 20-25 parts of dendrobe, 15-20 parts of radix ophiopogonis, 20-25 parts of radix rehmanniae recen, 15-20 parts of stiff silkworm, 20-25 parts of coptis chinensis, 20-25 parts of pseudo-ginseng, 15-20 parts of Chinese yam, 15-20 parts of endothelium corneum gigeriae galli, 15-20 parts of cinnamon, 15-20 parts of rhizoma atractylodis and 20-25 parts of winged euonymus twig.
4. The method according to claim 1, wherein the Chinese medicinal composition is prepared from the following Chinese medicines in parts by weight: 18 parts of codonopsis pilosula, 23 parts of astragalus membranaceus, 23 parts of radix scrophulariae, 23 parts of dendrobe, 18 parts of radix ophiopogonis, 23 parts of radix rehmanniae recen, 18 parts of stiff silkworm, 23 parts of coptis chinensis, 23 parts of pseudo-ginseng, 18 parts of Chinese yam, 18 parts of endothelium corneum gigeriae galli, 18 parts of cinnamon, 18 parts of rhizoma atractylodis and 23 parts of winged euonymus twig.
5. The method of claim 1, wherein the weight ratio of the powder to the petrolatum is 100: 25 to 35.
6. The method of claim 1, wherein the weight ratio of the powder to the petrolatum is 100: 30.
7. the method of claim 1, wherein the weight ratio of powder to azone is 100: 2 to 4.
8. The method of claim 1, wherein the weight ratio of powder to azone is 100: 3.
CN201910022246.1A 2019-01-10 2019-01-10 Detection method of traditional Chinese medicine composition for treating diabetes Active CN109568496B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910022246.1A CN109568496B (en) 2019-01-10 2019-01-10 Detection method of traditional Chinese medicine composition for treating diabetes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910022246.1A CN109568496B (en) 2019-01-10 2019-01-10 Detection method of traditional Chinese medicine composition for treating diabetes

Publications (2)

Publication Number Publication Date
CN109568496A CN109568496A (en) 2019-04-05
CN109568496B true CN109568496B (en) 2021-03-30

Family

ID=65916517

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910022246.1A Active CN109568496B (en) 2019-01-10 2019-01-10 Detection method of traditional Chinese medicine composition for treating diabetes

Country Status (1)

Country Link
CN (1) CN109568496B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692920A (en) * 2004-06-22 2005-11-09 周明忠 Medicine for treating diabetes
CN103610903A (en) * 2013-11-13 2014-03-05 李跃 Plaster for treating type II diabetes mellitus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102138964A (en) * 2010-02-03 2011-08-03 天津中新药业集团股份有限公司隆顺榕制药厂 Quality control method of Tongbaole chewing tablet
CN104407092A (en) * 2014-08-29 2015-03-11 湖南九典制药有限公司 Quality detection method of traditional Chinese medicinal composition with efficacy of appetizing and invigorating spleen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1692920A (en) * 2004-06-22 2005-11-09 周明忠 Medicine for treating diabetes
CN103610903A (en) * 2013-11-13 2014-03-05 李跃 Plaster for treating type II diabetes mellitus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张惠琴.中医药治疗糖尿病的体会.《中原医刊》.1998, *
莫娜.中医药治疗2型糖尿病胰岛素抵抗用药分析.《陕西中医》.2009, *

Also Published As

Publication number Publication date
CN109568496A (en) 2019-04-05

Similar Documents

Publication Publication Date Title
CN102784363A (en) Traditional Chinese medicine composition for treating pancreatic cancer and preparation method thereof
CN106359720A (en) Health protection tea for clearing heat from throat and moistening lung and preparation method thereof
CN112336815A (en) Anti-aging composition for strengthening body, preventing cancer and conditioning hypertension, hyperglycemia and hyperlipidemia and application thereof
CN103479963A (en) Traditional Chinese medicine capsules for treating rheumatoid arthritis and preparation method thereof
Li Chinese herbal medicine
CN108813501B (en) Health-preserving honey paste with functions of clearing heat, moistening lung, relieving cough, reducing phlegm, relieving asthma and regulating human body functions
CN104306659B (en) A kind of Chinese medicine composition for treating diabetes B
CN106177477A (en) A kind of health composition for blood sugar lowering, blood fat reducing and blood pressure lowering
CN105343593A (en) Spica prunellae health oral liquid capable of decreasing blood pressure and blood lipid as well as preparation method of spica prunellae health oral liquid
KR101028073B1 (en) Natural herb medicine composition improving blood circulation and immune activity of human body and its process of production
CN107812115A (en) A kind of Chinese medicine composition for treating diabetes
CN109568496B (en) Detection method of traditional Chinese medicine composition for treating diabetes
CN103861041B (en) A kind of Chinese medicine composition for the treatment of acute and chronic rhinitis and preparation method thereof
Roy et al. Herbs Used In Peptic Ulcer: A Review.
CN105561098A (en) Medicine for treating erosive beriberi
CN105617268A (en) Medicine for treating scale keratinization type beriberi
CN109528984A (en) A kind of cream formula and preparation method by smearing breast treatment mammary gland disease
CN104606570A (en) Consumptive thirst tea substitute drink suitable for diabetes patients and preparation method of consumptive thirst tea substitute drink
CN109646665A (en) Cortex Eucommiae clearing lung-heat anticancer solid tea and preparation method thereof
CN108714167B (en) A Chinese medicinal preparation for external use for treating vitiligo, and its preparation method
CN114712470B (en) Medicine for treating deficiency of kidney of men and preparation method thereof
CN107115456A (en) Yin-nourishing pill for smoothing intestines and preparation method thereof
CN106213514A (en) A kind of chrysanthemum health-care oral liquid improving immunity and preparation method thereof
CN106039075A (en) Traditional Chinese medicine composition and method for treating lung and stomach heat accumulated type chronic pharyngitis
CN105560694A (en) Medicine composition for treating diabetic nephropathy and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant