WO2017052269A1 - Hair growth promoting and anti-inflammatory composition comprising composite extract of natural products - Google Patents

Abstract

The present invention relates to a hair growth promoting and anti-inflammatory composition comprising a composite extract of natural products and, more particularly, to a hair growth promoting and anti-inflammatory food or cosmetic composition comprising a composition including an alfalfa extract and a kohlrabi extract, and additionally further comprising at least one extract selected from the group consisting of a broccoli extract, a cistanche tubulosa extract and a kelp extract. The composition of the present invention can be used usefully for developing functional foods and cosmetics that prevent hair loss and dandruff and promote hair growth since the composition has an effect of activating factors which promote hair growth in skin and increasing the expression thereof, thereby creating a skin environment which promotes hair growth, and having an excellent effect of suppressing skin inflammation.

Description

 【Specification】

 [Name of invention]

 Hair growth promoting and anti-inflammatory composition comprising a natural product complex extract

 The present invention relates to a hair growth promoting and anti-inflammatory composition comprising a natural product complex extract, more specifically, a composition comprising alfalfa extract and Javierbi extract and broccoli extract, sistane tubulosa extract and kelp extract It relates to a hair growth promoting and anti-inflammatory food or cosmetic composition further comprising one or more extracts selected from the group consisting of.

Background Art

This application claims the priority of Korean Patent Application No. 10-2015-0134540, filed on September 23, 2015, the entirety of which is a reference of the present application. Hair is a protein filament that grows in the hair follicle of the skin dermis. Hair follicles are largely composed of dermal papilla, matrix surrounding dermal papilla, and root sheath. The dermal papilla is mainly composed of connective tissue and capillaries, and surrounds the hair root to supply nutrients and oxygen. The matrix is composed of epithelial cells and melanocytes, and actively divides cells. Here, the divided cells are keratinized, causing hair to grow and grow out of the skin. Root sheath has inner root sheath and outer root sheath, and it protects the growing hair until it is fully aerated and reaches the skin epidermis. The hair grows, falls out and regrows periodically through the growth, anagen, catagen, telogen, and defoliation phases. The hair grows in the hair follicles as hair grows, and the fungal cells in the hair follicle bulge are activated to form new substrates during the initial growth phase, and the hair shaft and hair shafts from the relatively undifferentiated basal cells. Myotium herb is produced. The size and length of the hair stem are proportional to the size of the hair follicle and the duration of the hair growth phase, respectively. Alopecia refers to a condition in which the hair density of a part that normally has hair is abnormally low or missing, and is classified into scars and non scars. Scar alopecia is a hair loss that causes hair follicles to break down and recovery to fibrous tissue, resulting in permanent hair loss. Scarring and infection from trauma, burns, tumors, and surgery are the main causes. Non-cancerous alopecia is a condition in which the tissue is not fibrous and hair follicles are preserved, and alopecia areata, resting / growth alopecia, feminine alopecia, and male hereditary androgen alopecia. The cause of hair loss is not known exactly, but environmental and genetic factors such as air pollution, dietary habits, stress, hormonal imbalance, autonomic nervous system and blood circulation disorders, excessive sebum production, malnutrition of hair roots, allergies, bacteria It is estimated that physiological factors such as infection and aging work in combination. Alopecia occurred only in the middle and middle age group in the past, but recently occurs frequently among young people in their 20s and 30s. The cosmetic or pharmaceutical research to improve hair loss and promote hair growth one has the feet is actively ^i, currently receive drug approval commercially available hair loss treatment of the Food and Drug Administration (FDA) has liniment of minoxidil (minoxidi l) and There is an oral finasteride (f inasteride). Minoxidil induces hair growth by expanding blood vessels to increase blood flow and nourish hair roots, while finasteride is a specific inhibitor of Π 5 α -reducing enzymes that prevents testosterone from converting to dihydrotestosterone It is known to suppress. These hair regrowth treatments are effective in preventing hair loss because they are effective only in the area where the hair roots are activated, but the growth effects of hairs that have been in the resting state for a long time are insignificant, or the effects last only during dosing. . In particular, drugs that regulate male hormones may cause side effects such as decreased libido and erectile dysfunction. Therefore, it is necessary to prevent hair loss, promote hair growth, maintain the effect stably, and develop safe and economical hair growth promoting technology with less side effects.

[Detailed Description of the Invention]

 [Technical problem]

Therefore, the inventors of the present invention while researching a natural substance that can promote hair growth and suppress skin inflammation that can cause hair loss, alfalfa sprout extract, cola bud sprout extract, broccoli sprout extract, cystane tubulosa extract ， Kelp Extract The present invention has been found to have an excellent hair growth promoting effect and an anti-inflammatory effect. Therefore, the object of the present invention

 It is to provide a food composition for promoting hair growth and anti-inflammatory, including alfalfa extract and kohlrabi extract.

 In addition, the object of the present invention

 It is to provide a hair growth promoting and anti-inflammatory food composition consisting of alfalfa extract and kohlrabi extract.

 In addition, the object of the present invention

 It is to provide a hair growth promoting and anti-inflammatory food composition consisting essentially of alfalfa extract and kohlrabi extract. Another object of the present invention

 It is to provide a hair growth promoting and anti-inflammatory cosmetic composition comprising alfalfa extract and kohlrabi extract.

 In addition, another object of the present invention

 It is to provide a cosmetic composition for promoting hair growth and anti-inflammatory consisting of alfalfa extract and kohlrabi extract.

 In addition, another object of the present invention

 It is to provide a cosmetic composition for promoting hair growth and anti-inflammatory consisting essentially of alfalfa extract and kohlrabi extract. Another object of the present invention

 It is to provide the use of alfalfa extract and cola rain extract for the preparation of agents for promoting hair growth and anti-inflammatory. Another object of the present invention

 It is to provide a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition comprising an alfalfa extract and a kohlrabi extract.

In addition, another object of the present invention To provide a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition consisting of alfalfa extract and kohlrabi extract.

 In addition, another object of the present invention

 It is to provide a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition consisting essentially of alfalfa extracts and kohlrabi extracts.

Technical Solution

 In order to achieve the above object the present invention

 Provided are food compositions for promoting hair growth and anti-inflammatory, including alfalfa extracts and kohlrabi extracts.

 In addition, in order to achieve the object of the present invention, the present invention

 It provides a hair growth promoting and anti-inflammatory food composition consisting of alfalfa extract and kohlrabi extract.

 In addition, in order to achieve the object of the present invention, the present invention

 It provides a composition for promoting hair growth and anti-inflammatory food consisting essentially of alfalfa extract and kohlrabi extract. In order to achieve the other object of the present invention, the present invention

 It provides a hair growth promoting and anti-inflammatory cosmetic composition comprising alfalfa extract and kohlrabi extract.

 In addition, in order to achieve the other object of the present invention, the present invention

 It provides a hair growth promoting and anti-inflammatory cosmetic composition comprising alfalfa extract and kohlrabi extract.

 In addition, in order to achieve another object of the present invention, the present invention

 It provides a hair growth promoting and anti-inflammatory cosmetic composition comprising alfalfa extract and kohlrabi extract. In order to achieve the other object of the present invention, the present invention

Provided are the use of alfalfa extracts and kohlrabi extracts for the preparation of agents for promoting hair growth and anti-inflammatory. In order to achieve the other object of the present invention,

 It provides a method for promoting hair growth and inhibiting inflammation comprising administering to a subject in need thereof an effective amount of a composition comprising an alfalfa extract and a kohlrabi extract.

 In addition, in order to achieve another object of the present invention, the present invention

 It provides a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition consisting of alfalfa extract and kohlrabi extract.

 In addition, in order to achieve another object of the present invention, the present invention

 It provides a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition consisting essentially of an alfalfa extract and a kohlrabi extract. Hereinafter, the present invention will be described in detail. The present invention provides a composition for promoting hair growth and anti-inflammatory foods comprising alfalfa extract and kohlrabi extract.

 The present invention also provides a composition for promoting hair growth and anti-inflammatory food consisting of alfalfa extract and kohlrabi extract.

The present invention also provides a composition for promoting hair growth and anti-inflammatory foods consisting essentially of alfalfa extract and kohlrabi extract. Food composition of the present invention comprises alfalfa extract and kohlrabi extract, at least one extract selected from the group consisting of broccoli extract, cystane tubulosa extract and kelp extract to further enhance hair growth and anti-inflammatory effect It may be to further include. Alfalfa extract, kohlrabi extract ᅳ and broccoli extract according to the present invention may be preferably extracted from each sprout. The present inventors have found that a combination composition of alfalfa sprout extract and kohlrabi sprout extract can be used in the skin gamma-glutamyl transpeptidase -glutamyl transpeptidase, alkaline phosphatase (ALP), epidermal growth factor (EGF), vascular endothelial cell growth. Hair growth as a factor (VEGF) Increasing the amount of activity and / or expression of the inducing factor to create a skin environment that promotes hair growth, it was found to have an excellent effect in inhibiting skin inflammation that can cause hair loss and dandruff. In particular, when the broccoli sprout extract, the sistan sibubula extract, the kelp extract is further included in the mixed composition, the hair growth promoting and anti-inflammatory effects are found to be further increased and disclosed for the first time in the present specification. By using the effects of the mixed extract composition of the plant and seaweed according to the present invention can be used to develop a safe and effective food for promoting hair growth and anti-inflammatory. Hair growth promoting and anti-inflammatory effects of the mixed extract composition of the plant and seaweed identified by the inventors are as follows.

In one embodiment of the present invention gamma-glutamyl transpeptidase -glutamyl transpeptidase known to induce hair growth (anagen) in the skin of mice administered a mixture of alfalfa sprout extract and kohlrabi sprout extract GTP) activity is markedly increased, and the effect of increasing the Y GTP activity is confirmed to increase even more when alfalfa sprouts and kohlrabi sprout extracts are added to broccoli sprout extracts, cystane tubulosa extracts, and kelp extracts. It was. _Y GTP is an enzyme that transfers gamma glutamyl functional groups, which are present in hair follicles and hair roots of hair follicles and greatly increase activity in hair growth phase (Kawabe TT et al., J Invest Dermatol, 103 (1): 122-6 , 1994). In another embodiment of the present invention, the activity of alkaline phosphatase (ALP) is markedly increased in the skin of a mouse administered with a combination composition of alfalfa sprout extract and kohlrabi sprout extract, broccoli sprout extract, cystan It was confirmed that the ALP activity was further increased when the body tubulosa extract, kelp extract was further administered. ALP is an enzyme that hydrolyzes phosphate groups, which is present in mesenchymal cells and epithelial cells of hair follicles, and greatly increases expression and activity in hair growth phase, especially in dermal papilla (I ida M et al. , Dev Growth Differ, 49 (3): 185-95, 2007). In another embodiment of the present invention, the expression of epidermal growth factor (EGF) that promotes hair growth by promoting cell proliferation and differentiation in the skin of mice to which a combination composition of alfalfa sprout extract and kohlrabi sprout extract is administered. Remarkably In addition, EGF expression was significantly increased when broccoli sprout extract, cystane tubulosa extract, and kelp extract were additionally administered. EGF increases the expression level in hair roots at the beginning of the hair growth period, induces hair follicles to progress to the growth phase, promotes the growth of the hair shaft (hai r shaft), and decreases the expression level in the catagen, resulting in hair growth phase. It plays an important role in the overall regulation of the hair growth cycle, such as allowing it to escape from the retrogression and subsequent hair growth cycles (Mak KK et al., J Biol Chem, 278 (28): 26120-6, 2003). In another embodiment of the present invention, the expression level of vascular endothel ial growth factor (VEGF) that promotes angiogenesis is remarkable in the skin of mice to which a combination composition of alfalfa sprout extract and kohlrabi sprout extract is administered. Increasingly, especially when broccoli sprout extract, cystane tubulosa extract, and kelp extract were added together, the expression of VEGF was significantly increased. VEGF promotes angiogenesis and blood circulation in hair follicles, thereby promoting hair growth and increasing hair follicle and hair size (Yano K et al., J Clin Invest, 107 (4): 409-417, 2001). Meanwhile, in another embodiment of the present invention, a mixture of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, and the like inhibits nitric acid monoxide (NO), an inflammatory mediator produced in macrophage RAW 264.7 cells. It was confirmed that there is a synergistic effect. In particular, the composite extract composition containing all five extracts, such as plants and seaweeds, had the effect of significantly lowering the NO production. In another embodiment of the present invention, the anti-inflammatory effect of the complex composition including alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and / or kelp extract Confirmed by mouse experiment. In mice treated with alfalfa sprout extracts, kohlrabi sprout extracts, or additionally broccoli sprout extracts, single doses of exudate produced by subcutaneous air infusion and skin inflammation caused by carageenan, an proinflammatory agent Excessive decrease in the number of mice treated with the exudation, the concentrations of inflammatory mediators N0 and prostaglandin E2 (prostaglandin E2, PGE ₂ ) in the exudates were also significantly lower. This effect was even better when it included all five plant and algae extracts. Experimental results of the above examples show that the combination of alfalfa sprout extract and kohlrabi sprout extract shows excellent hair growth factor regulation and anti-inflammatory effect, in particular, alfalfa sprout extract and kohlrabi sprout extract, ¥ brobroccoli sprout extract, sistane tubulosa extract And / or kelp extracts show that the effect is even more elevated. Therefore, a combination of alfalfa extract and kohlrabi extract or complex extract composition further comprising broccoli extract, cystane tubulosa extract, and / or kelp extractol may cause hair growth promoting effect and cause hair loss and dandruff. It can be seen that it is possible to develop functional foods with excellent anti-inflammatory effects. As used herein, "hair" refers to hair, hair, facial hair and / or hair, which includes hair, eyelashes, eyebrows, mustache, beard, ear hair, nose hair, breast hair, pubic hair, auxiliary hair, and the like. It is not limited. 'Promoting hair growth' of the present specification promotes hair follicle formation, structural stabilization of hair follicles and smoothing of nutrient and oxygen supply, faster induction of new hair growth phase, extension of hair growth phase, increase of hair growth rate, and / Or increases in hair density, including but not limited to induction of hair growth and making it more visible to the eye. 'Inflammation' in the present invention inhibits the cellular cellular inflammation response such as immune cell infiltration, secretion of pro-inflammatory cytokines, pro-inflammatory progelandine and pro-inflammatory factors such as nitric oxide (NO), erythema, keratinization To prevent, alleviate or improve symptoms such as increased skin thickness, stratification, fever and pain. Excessive inflammatory reactions on the skin can cause hair loss and dandruff. In the present invention, 'al fal fa (Medicago sa va)' is a perennial plant of legumes also called alfalfa in Korea, and is widely cultivated as food, medicinal and mainly animal feed crops. Alfalfa in the present invention may be alfalfa or sprouts of alfalfa. In the present invention 'kohl rabi' (kohl rabi) is a gonzloides in Brassica leracea (Brass j ^' ca oleracea spp Gongy l odes) A plant belonging to the group, also known as German turnip or turni p cabbage. Kola leaves and light green or purple bulbs (tube stems) are used for food. The kohlrabi in the present invention may be kohlrabi or sprout of kohlrabi. In the present invention, 'broccol i (broccol i)' is a plant belonging to the Brassica oleracea OSrass / ca, also called green cauliflower. One of the cabbage-eating cabbages began to be bred in Italy, where it was bred to suppress the development of flowers, giving it a present appearance with numerous f lorets from thick stems (st alk). Both stem and flower eyes are eaten. Broccoli in the present invention may be broccoli or sprout of broccoli. In the present invention, 'cystane tubulosa tubulosaY is a parasitic plant of the desert belonging to the genus Cistanche, also referred to as mandarin breeding. It lacks chlorophyll, so it grows by absorbing water and nutrients from host plants. The stems of the sistanche tubulosa before germination were cistanche salsa, Ystanz desert desert ico la, and the sistanche ambigua

Along with ambigua, it is a herbal medicine name for breeding and has been used as an herbal medicine for infertility and nursing care. In the present invention, 'Damashima (5ac / 2a japonicaY is a marine perennial large seaweed distributed on the Pacific coast. It was also called Laminaria japonica, Laminaria ochotensis, etc. according to the previous classification method. It is rich in inorganic salts such as iodine, etc. The alfalfa, kohlrabi, broccoli, cystane tubulosa and kelp extracts are selected from the appropriate extraction solvents, and are known in the art for acid / base extraction, hot water extraction and room temperature extraction. , Extraction, reflux, angle extraction, ultrasonic extraction, pressurized heating extraction, low temperature and high pressure extraction, enzymatic extraction, solvent extraction, etc. can be used without limitation, etc. As extraction solvent, water, methane, Alcohols having 1 to 6 carbon atoms, such as ethanol, alcohol, propanol, isopropane, butanol, pentane, butoxyethane, ethylene Recall, acetone, ether, diethyl ether, petroleum ether (pet rol eum ether), chloroform, ethyl acetate It can be extracted according to a known extraction method using any one or a mixed solvent thereof selected from known conventional solvents such as ethylene, methylene chloride, n-nucleic acid (hexane), cyclonucleic acid, acetone, benzene.

 Preferably, the extract of the present invention may be extracted with any one extraction solvent selected from the group consisting of water, alcohol having 1 to 6 carbon atoms, alcohol and mixed solvents thereof, and most preferably water or ethanol aqueous solution as the extraction solvent. can do. In addition, the extract of the present invention may be an extract extracted primarily by the extraction method known in the art, or may be mixed with the primary extract by re-extracting the extraction residue after the primary extraction in order to increase the extraction efficiency. In addition, the extract of the present invention is purified by fractionation and separation by chromatography, ultrafiltration, ultrafiltration (membrane separation, ultrafilteration) or the like according to methods known in the art to remove impurities and increase the concentration of the active ingredient. It may be a filtration process. The final extract can be concentrated using well-known concentration methods such as precipitation concentration, evaporation concentration, azeotropic concentration, reduced pressure concentration, distillation concentration, centrifugation, reverse osmosis method, and concentrator. The solvent can be removed and solidified to form a powder. For example, the extract of the present invention is extracted using an aqueous solvent of water or ethanol as an extraction solvent, the extract is filtered to remove impurities, and then the extraction solvent is removed using a vacuum evaporator, followed by column chromatography. Partial purification using and hot-air dried may be prepared through a process of obtaining the extract powder. Specific preparation method of the extract used in the embodiment of the present invention is as described in the experimental method of the embodiment. The food composition of the present invention preferably contains an alfalfa extract and a kohlrabi extract in a weight ratio of 1: 0.1 to 10 for hair growth promotion and anti-inflammatory effects. Specifically, for example, the mixing ratio of the alfalfa extract and the kohlrabi extract, 1: 0.1,

1: 0.2, 1: 0.3, 1: 0.4, 1: 0.5, 1: 0.6, 1:: 0.7, 1: 0, .8, 1:: 0.9, 1:: 1.0, 1: 1.1, 1: 1.2

1: 1.3, 1: 1.4, 1: 1.5, 1: 1.6, 1: 1.7, 1: 1.8, 1: 1 .9. 1: 2.0 ， 1 ：: 2.1 ， 1: 2.2 ， 1: 2.3 '

1: 2.4, 1: 2.5, 1: 2.6, 1: 2.7, 1 ： 2.8, 1 :： 2.9, 1: 3, .0 ， 1 :： 3.1 ， 1 :： 3.2, 1: 3.3 ， 1: 3.4 ，

1: 3.5, 1: 3.6, 1: 3.7, 1: 3.8, 1: 3.9, 1:: 4.0, 1: 4, .1, 1:: 4.2, 1:: 4.3, 1: 4.4, 1: 4.5,

1: 4.6, 1: 4.7, 1: 4.8, 1: 4.9, 1: 5.0, 1:: 5.1, 1: 5,, 2, 1:: 5.3, 1: 5.4, 1: 5.5, 1: 5.6 1: 5.7 '1: 5.8, 1: 5.9, 1: 6.0, 1: 6.1, 1: 6.2, 1: 6.3, 1: 6.4, 1: 6.5, 1: 6.6, 1: 6.7, 1: 6.8, 1: 6, 9, 1: 7.0, 1: 7.1, 1: 7.2, 1: 7.3, 1: 7.4, 1: 7.5, 1: 7.6, 1: 7.7, 1: 7.8, 1: 7.9, 1: 8.0, 1: 8.1, 1: 8.2, 1: 8.3, 1: 8.4, 1: 8.5, 1: 8.6, 1: 8.7, 1: 8.8, 1: 8.9, 1: 9.0, 1: 9.1, 1: 9.2, 1: 9.3, 1: 9.4, 1: 9.5, 1: 9.6, 1: 9.7, 1: 9.8, 1: 9.9, 1:10 may be in an increase ratio, it is not limited to the above numerical value within the range of 1: 0.1 to 10 by weight. Preferably, alfalfa extract and kohlrabi extract are included in a 1: 1 fertilization ratio. In addition, in order to further promote hair growth and anti-inflammatory effect, the food composition of the present invention is one or more extracts selected from the group consisting of alfalfa extracts and kohlrabi extracts, broccoli extracts, cystane tubulosa and kelp extracts. It may further include. When the alfalfa, kohlrabi, broccoli, cystanche tubulosa and kelp extracts are included, the composite extract composition has a single extract of 1: 0.1-10: 0.1-5: 0.1-30: 0.1-10 It is preferably included in the weight ratio. Specifically, when alfalfa, kohlrabi, broccoli, cystane tubulosa and kelp extracts are all included, for example, the ratio of the complex extract composition, the kohlrabi extract is 0.1, 0.2, 0.3, 1 part by weight of alfalfa extract. 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, alfalfa extract 1 If it is within the range of 0.1 to 10 parts by weight relative to parts by weight, the number is not limited.

 In addition, broccoli extract is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, relative to 1 part by weight of alfalfa extract. 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, It may be 4.6, 4.7, 4.8, 4.9, 5.0, it is not limited to the above numerical value within the range of 0.1 to 5 parts by weight relative to 1 part by weight of alfalfa extract.

In addition, the cystane tubulosa extract is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1 part by weight of alfalfa extract. 1.8, 1.9 2.0, 2.1, 22, 2.3, 2.4 2.5, 2.7, 2.8 3.0 3.2,

3.3, 3.4 3.5, 3.6, 3 7, 3.8, 3.9 4.0, 4.2, 4.3 4.5 4.7,

4.8, 4.9 5.0, 5.1, 52, 5.3, 5.4 5.5, 5.7, 5.8 6.0 6.2,

6.3, 6.4 6.5, 6.6, 6 7, 6.8, 6.9 7.0, 7.2, 7.3 7.5 7.7,

7.8, 7.9 8.0, 8.1, 8 2, 8.3, 8.4 8.5, 8.7. 8.8 9.0 9.2,

9.3, 9.4 9.5, 9.6, 9.7, 9.8, 9.9, 10.0. 10.1. 10.2. 10. 10. 10.5. 10.6.

10.7 10 8. 10 9. 11.0 11 11 11.3 11.4. 11.5. 11 11 11.8 11.9, 12.0 12 12, 12 12. 12, 12 12, 12, 12 13, 13.1 13.2, 13.3 13 13. 13 13, 13, 13 14, 14, 14 14, 14.4 14.5, 14.6 14 14, 14 15, 15 15, 15 15, 15 15, 15.7 15.8, 15.9 16 16. 16 16. 16, 16. 16, 16. 16 16. 17.0 17.1, 17.2 17 17. 17 17, 17, 17, 17, 18. 18 18, 18.3 18.4, 18.5 18 18. 18 18, 19, 19 19, 19. 19 19, 19.6 19.7, 19.8 19 20. 20 20. 20, 20, 20. 20. 20 20, 20.9 21.0, 21.1 21 21 21 21, 21. 21, 21, 21, 22 22, 22.2 22.3, 22.4 22 22. 22 22. 22, 23, 23, 23. 23 23, 23.5 23.6, 23.7 23 23. 24 24. 24, 24, 24, 24, 24 24. 24.8 24.9, 25.0 25, 25. 25 25. 25. 25. 25. 25. 25, 26. 26.1 26.2, 26.3 26, 26. 26 26. 26. 26. 27. 27. 27 , 27. 27.4 27.5, 27.6 27, 27. 27 28. 28. 28. 28. 28. 28, 28. 28.7 28.8, 28.9 29. 29. 29 29. 29.4 29. 29. 29. 29. 29. 30.0 It can be combined, if the range of 0 to 30 parts by weight relative to 1 part by weight of alfalfa extract is not limited to the above numerical value.

The kelp extract is 0.1, 0.2 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7 1, .8, 1.9 with respect to 1 part by weight of alfalfa extract. , 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2 3, .3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3 , 4.4, 4.5, 4.6, 4.7 4. .8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2 6, .3, 6.4, 6.5, 6.6 , 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0 , 9.1, 9.2 9. .3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, alfalfa extract 1 part by weight ᄇ ^' ] limited to the above values within the range of 0.1 to 10 parts by weight. It doesn't work. Preferably, the composite extract composition comprising all of the extracts of alfalfa, kohlrabi, broccoli, cystane tubulosa and kelp is used as the single extract of each of the extracts from 1: 0.1 to 10: 0.1-5: 10.1-30. : 0.01 to 10 by weight, more preferably 1: 1: 0.5: 3: 1 by weight.

 In addition, even if the broccoli extract, cystane tubulosa extract, any one or two extracts of kelp extract further comprises the weight ratio corresponding to each extract in the weight ratio of the complex extract composition (for example For example, when the composition of the present invention contains alfalfa, kohlrabi and broccoli extract, the weight ratio of each may be included in 1: 0.1-10: 0.1-5).

As described above, the alfalfa extract, kohlrabi extract and broccoli extract may be preferably extracted from each sprout. The composition for food using the composite extract composition according to the present invention includes all forms such as a functional food (funct ional food), nutri t ional supplement and health food (heal th food). These types can be prepared in various forms according to conventional methods known in the art. For example, as a health food, the food composition itself of the present invention may be prepared by drinking tea, juice and drink, or granulated, capsulated and powdered. In addition, the food composition of the present invention can be prepared in the form of a composition by mixing with known substances or active ingredients known to have the effect of promoting hair growth and anti-inflammatory. Functional foods also include beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned foods, jams, marmalade, etc.), fish, meat and processed food products (e.g. hams, sausages). Cornflakes, etc.), breads and noodles (e.g. udon, soba noodles, ramen, spaghetti, macaroni, etc.), fruit juices, various drinks, cookies, candy, dairy products (e.g. butter, cheese), edible vegetable oils, It can be prepared by adding the food composition of the present invention to margarine, vegetable protein, retort food, copper food, various seasonings (for example, miso, soy sauce, sauce, etc.). The preferred content of the food composition according to the present invention is not limited thereto. Preferably from 0.01 to 50 weight ¾ of the total weight of the food finally produced. In order to use the food composition of the present invention in the form of a food additive, it may be prepared and used in powder or concentrate form. The present invention provides a composition for promoting hair growth and anti-inflammatory cosmetics comprising alfalfa extract and kohlrabi extract.

 The present invention also provides a cosmetic composition for promoting hair growth and anti-inflammatory consisting of alfalfa extract and kohlrabi extract.

 In another aspect, the present invention provides a hair growth promoting and anti-inflammatory cosmetic composition consisting essentially of alfalfa extract and kohlrabi extract. The alfalfa extract and the kohlrabi extract are preferably included in the cosmetic composition in a weight ratio of 1: 0.1 to 10 for the effect of promoting hair growth and anti-inflammatory. As a specific volume _o, for example, the mixing ratio of palpa extract and kohlrabi extract is 1: 0.1.

1: 0. 2, 1 0 3, 1: 0.4, 1: 0.5, 1: 0.6, 1: 0.7, 1 0 .8, 1: 0.9, 1 1.0, 1: 1.1, 1: 1.2,

1: 1. 3, 1 1 4, 1: 1.5, 1: 1.6, 1: 1.7, 1: 1.8 '1 1 .9. 1: 2.0 ， 1 2.1, 1: 2.2 ， 1: 2.3 ，

1: 2. 4, 1 2 5, 1: 2.6, 1: 2.7, 1: 2.8, 1: 2.9, 1 3.0, 1: 3.1, 1 3.2, 1: 3.3, 1: 3.4,

1: 3. 5, 1 3 6, 1: 3.7, 1: 3.8, 1: 3.9, 1: 4.0, 1 4.2, 1 4.2, 1 4.3, 1: 4.4, 1: 4.5,

1: 4. 6, 1 4 7, 1: 4.8, 1: 4.9, 1: 5.0, 1: 5.1, 1 5 .2, 1 5.3, 1 5.4, 1: 5.5, 1: 5.6

1: 5. 7, 1 5 S, 1: 5.9, 1: 6.0, 1: 6.1, 1: 6.2, 1 6, 3, 1 6.4, 1 6.5, 1: 6.6, 1: 6.7,

1: 6. s, 1 6 9, 1: 7.0, 1: 7.1, 1: 7.2, 1: 7.3, 1: 7: 4, 1 7.5, 1 7.6, 1: 7.7, 1: 7.8,

1: 7. 9, 1 8 0, 1: 8.1, 1: 8.2, 1: 8.3, 1: 8.4, 1: 8.5, 1 8.6, 1 8.7, 1: 8.8, 1: 8.9,

1: 9.0, 1: 9.1, 1: 9.2, 1: 9.3, 1: 9.4, 1: 9.5, 1: 9.6, 1: 9.7, 1: 9.8, 1: 9.9, 1:10 If it is in the range of 0.1 to 10 weight ratio, it will not restrict | limit to the said numerical value. Preferably, alfalfa extract and kohlrabi extract are mixed in a 1: 1 witness ratio. In addition, the cosmetic composition according to the present invention further comprises at least one extract selected from the group consisting of broccoli extract, cystane tubulosa extract and kelp extract in addition to alfalfa and kohlrabi extract to further maximize the effect of hair growth and anti-inflammatory. There is even more to include. When all three extracts are included, alfalfa extract, kohlrabi sprout extract, broccoli extract, cystane tubulosa extract, and kelp extract are 1: 0.1-10: 0.1-5: 0.1-30: 0.1- It is preferable to be contained in cosmetics by the weight ratio of ten. Specifically, alfalfa, kohlrabi, broccoli, poems If it contains both extracts of stanche tubulosa and kelp, the ratio of the composite extract composition is 예로 cotton cola sa ᄅ L Ξ. -0.1, 0.2, 0.3

0.4, 0, 5, 0, 6, 0, .7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3 _; , 1.4, 1.5, 1.6, 1.7, 1.8,

1.9, 2.0, 2, • 1, 2, .2, 2.3, 2.4, 2.5, 2.6 '2.7, 2.8 _; , 2.9, 3.0, 3.1, 3.2, 3.3 ，

3.4, 3, .5, 3, .6, 3. .7, 3.8, 3.9, 4.0, 4.1, 4.2, 4, .3 _; , 4.4, 4.5, 4.6, 4.7, 4.8,

4.9, 5, .0, 5, .1, 5,, 2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8,, 5.9, 6.0, 6.1, 6.2, 6.3,

6.4, 6, .5, 6, 6, 6, 7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3 _; , 7.4, 7.5, 7.6, 7.7, 7.8,

7.9, 8.0, 8, .1, 8, .2, 8.3, 8.4, 8.5, 8.6, 8.7 _: '8, 8,, 8.9, 9.0, 9.1, 9.2, 9.3,

9.4, 9., 5, 9. 6, 9. 7, 3, 9.8, 9.9, 10.0, compared to one increase of alfalfa extract

If it is within the range of 0.1 to 10 parts by weight, it is not limited to the above numerical value.

 In addition, broccoli extract is 0.1, 0.2, 0.3, 0.4, compared to 1 part by weight of alfalfa extract

0.5, 0.6 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, and 0.1 to 1 part by weight of alfalfa extract. If it is in the 5 weight part range, it will not restrict | limit to the said numerical value.

 In addition, cystane tubulosa extract is 0.1, 0.2,

0.3, 0.4, 0.5, 0.6, 0, .7, 0.8 _; , 0 .9, 1.0, 1.1, 1.2, 1 • 3, 1. Λ, 1. 5, 1.6, 1.7

1.8, 1.9, 2.0, 2.1, 2, .2, 2.3 _: '2 • 4, 2.5, 2.6, 2.7, 2 • 8, 2. 9, 3. 0, 3.1', 3.2

3.3, 3.4, 3.5, 3.6, 3, .7, 3.8 _; , 3 • 9, 4.0, 4.1, 4.2, 4 • 3, 4. 4, 4. 5, 4.6,, 4.7,

4.8, 4.9, 5.0, 5.1, 5.2, 5.3 _:, 5 • 4, 5.5, 5.6, 5.7. , 5 .8, 5. 9, 6. 0, 6.1, 6, .2,

6.3, 6.4, 6.5, 6 • 6, 6. .7, 6.8 _; , 6 • 9, 7.0, 7.1, 7.2,, 7 • 3, 7. 4, 7. 5, 7.6, 7, .7,

7.8, 7.9, 8.0, 8.1, 8. .2, 8.3,, 8.4, 8.5, 8.6, 8.7,, 8 • 8, 8. 9, 9. 0, 9.1, 9, .2,

9.3, 9.4, 9.5, 9. 6, 9.7, 9.8, 9.9, 10.0. 10.1. 10. 2. 10. 3. 10. .4. , 10.5. 10., 6.

10.7. 10. 8. 10., 9. 11 .0,. 11.1. 11. 2. 11.3. 11.4. , 11 .5. 11 .6. 11 .7, 11.8, 11, .9,

12.0, 12. 1, 12. ■ 2, 12 .3 _:, 12.4, 12. 5, 12.6, 12.7,, 12 .8, 12 • 9, 13.0, 13.1, 13,, 2,

13.3, 13. 4, 13. 5, 13 .6 _; , 13.7, 13. 8, 13.9, 14.0,, 14, .1, 14, .2, 14 .3, 14.4, 14. ^, 5,

14.6, 14. 7, 14. 8, 14 .9 _; , 15.0, 15. 1, 15.2, 15.3,, 15, .4, 15, .5, 15 .6, 15.7, 15., 8,

15.9, 16. 0, 16. 1, 16 .2 _; , 16.3, 16. 4, 16.5, 16.6,, 16, .7, 16, .8, 16 .9, 17.0, 17. .1 '

17.2, 17. 3, 17. 4, 17 .5,, 17.6, 17. 7, 17.8, 17.9, 18, ■ 0, 18. .1, 18 .2, 18.3, 18. 4,

18.5, 18. 6, 18. 7, 18 .8,, 18.9, 19. 0, 19.1, 19.2, 19. ■ 3, 19. .4, 19 .5, 19.6, 19.

19.8, 19. 9, 20. 0, 20 • 1,, 20.2, 20. 3, 20.4, 20.5, 20. .6, 20. .7, 20 .8, 20.9, 21. 0, 21.1, 21.2,, 21..3, 21.4, 21.5, 21, 6, 21.Ί, 21.8, 21.9, 22..0, 22.1, 22,, 2, 22.3,

22.4, 22 .5 _; , 22. .6, 22.7, 22.8, 22 • 9, 23. 0, 23 .1, 23.2, 23, .3, 23. 4, 23. .5, 23.6,

23.7, 23 .8 _:, 23, .9, 24.0, 24.1, 24 .2, 24. 3, 24 • 4, 24.5, 24. .6, 24. 7, 24, .8 '24.9,

25.0, 25 .1 _:, 25, .2, 25.3, 25.4, 25 .5, 25. 6, 25 .7, 25.8, 25, .9, 26. 0, 26., 1, 26.2,

26.3, 26 Λ,, 26., 5, 26.6, 26.7, 26 .8, 26. 9, 27 .0, 27.1, 27. .2, 27. 3, 27. .4, 27.5,

27.6, 27 .1,, 27, .8, 27.9, 28.0, 28 .1, 28. 2, 28 .3, 28.4, 28.. 5, 28.6, 28., 7, 28.8,

28.9, 29 .0 _; , 29. .1, 29.2, 29.3, 29 .4, 29. ⁵ , 29 .6, 29.7, 29. .8, 29. 9, 30. .0.

 丁. It is not limited to the above numerical value within the range of 0.1 to 30 parts by weight ᄋ 1 relative to 1 part by weight of alfalfa extract.

 It is also 0.1, 0.2, 0.3 ， 0.4 ， 1 weight part of kelp "extract", "U", alfalfa "extract", "water"

0.5, 0 • 6, 0.7 ，. 0.8, 0.9, 1.0, 1.1,, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9,

2.0, 2 • 1, 2.2, 2.3, 2.4, 2, .5, 2.6,, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4,

3.5, 3 • 6, 3.7, 3.8, 3.9, 4, .0, 4.1,, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9,

5.0, 5 • 1, 5.2, 5.3, 5.4, 5, .5, 5.6,, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2 _:, 6.3, 6.4,

6.5, 6.6, 6.7, 6.8, 6.9, 7, .0, 7.1,, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7 _; , 7.8, 7.9,

8.0, 8.2, 8.2, 8.3 _; , 8.4, 8, .5, 8.6,, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2 _: '9.3, 9.4,

9.5, 9. 6, 9.7, 9.8, 9.9, 10. 0 days

丁 There are: 0, .1 to 10 parts by weight relative to 1 part by weight of palpa extract ¹ side is not limited to the above values.

 Preferably, the composite extract composition comprising all of the extracts of alfalfa, kohlrabi, broccoli, cystane tubulosa, and kelp has a single extract of 1: 0.1-10: 0.1-5: 0.1-30: 0.1-10 It is included in the weight ratio of, More preferably, it is contained in 1: 1: 1: 0.5: 3: 1 weight ratio.

 In addition, even if the broccoli extract, cystane tubulosa extract, kelp extract any one or two extracts further comprises the weight ratio corresponding to each extract in the weight ratio of the composite extract composition can be applied as it is (for example For example, when the composition of the present invention comprises alfalfa, kohlrabi and broccoli extract, each weight ratio may be included in 1: 0.1-10: 0.1-5).

As described above, the alfalfa extract, kohlrabi extract and broccoli extract may be preferably extracted from each sprout. The extraction method and the extraction solvent of the extract are as described in the food composition of the present invention. The cosmetic composition using the extract composition according to the present invention may be prepared in any formulation commonly prepared in the art, and in addition to the extract, dermatologically acceptable media, bases, supplements, excipients, etc. Commercially prepared basic cosmetic compositions (such as face water, cream essences, cleansing foams and cleansing water, face packs, body oils), color cosmetic compositions (foundation, lipstick, mascara, makeup base), product compositions for scalp or hair ( Shampoo, conditioner, hair conditioner, hair gel, hair cream, hair pack, hair mask, hair essence) and soap. The cosmetic composition according to the present invention may preferably be prepared as a composition for hair or scalp such as eyelids, eyelashes, and hair. Examples of the excipient include, but are not limited to, emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, emollients, thickeners, thickeners, gelling agents, suspending agents, stabilizers, foaming agents, interfaces Active agents, layering agents, and solvents. In addition, fragrances, pigments, fungicides, antioxidants, preservatives, adjuvants and moisturizers may be further included, and may include thickeners, inorganic salts, synthetic polymers and the like for the purpose of improving physical properties. For example, when the face wash and soap are manufactured with the cosmetic composition of the present invention, the complex extract composition according to the present invention may be easily added to a conventional face wash and soap base. When the cream is prepared, it can be prepared by adding the extract of the present invention to a cream base of a general oil-in-water type (0 / W). To this, fragrances, chelating agents, pigments, antioxidants, preservatives and the like, synthetic or natural materials such as proteins, minerals, vitamins for the purpose of improving the physical properties can be added. Formulations of suitable cosmetic compositions include, for example, solution gels, solid or pasty anhydrous products, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), nonionics obtained by dispersing an oil phase in an aqueous phase. They may be provided in the form of vesicular dispersants, creams, skins, lotions, powders, ointments, sprays or conceal sticks. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. The content of the cosmetic composition according to the present invention is not limited thereto, but is preferably 0.001 to 50% by weight based on the total weight of the finally prepared cosmetic. The weight If the content of the extract is less than 0.001 weight ¾ of the total amount of the cosmetics, it is not preferable to achieve the desired hair growth promotion and anti-inflammatory effects sufficiently, and if it exceeds 50% by weight, the stability of the cosmetics is a problem. It may cause, it is not preferable because of the disadvantage of not expressing the cosmetic effect or sufficient cosmetic effect of the use cosmetics. The present invention provides the use of alfalfa extracts and kohlrabi extracts for preparing hair growth promoting and anti-inflammatory agents. The present invention provides a method for promoting hair growth and inhibiting inflammation, comprising administering to a subject in need thereof an effective amount of a composition comprising an alfalfa extract and a kohlrabi extract. The term 'effective amount' of the present invention refers to the amount of hair growth promoting and inflammation improvement, treatment, prevention, detection, and diagnostic effect when administered to an individual, and the term 'individual' refers to an animal, preferably a mammal, particularly a human. It may be an animal including, cells, tissues, organs and the like derived from the animal. The subject may be a patient in need of the effect. The formulation or composition of the present invention may be in the form of a food composition, cosmetic composition, pharmaceutical composition, etc., the food composition and the cosmetic composition is the same as described above.

The 'pharmaceutical composition' is a pharmaceutically acceptable carrier commonly used in the preparation of carbohydrate compounds (e.g., lactose, amylose, dextrose, sucrose, sorbbi, manni, starch, cellulose, Acacia rubber, calcium phosphate, alginate, gellatin, calcium silicate, microcrystalline cellulose, polyvinylpyridone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oil (e.g. Corn oil, cotton seed oil, soybean oil, olive oil, coconut oil), polyethylene glycol, methyl cellulose, methylhydroxy benzoate, propylhydroxy ¾ Zoate, talc, magnesium stearate and mineral oil. And, may include, but are not limited to, lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives and the like. Suitable pharmaceutically acceptable carriers and formulations are described in Remington's Pharmaceutical Sciences (19th ed., 1995). It is described in detail in.

 In addition, the pharmaceutical composition may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous infusion, subcutaneous injection, intramuscular injection, skin application, and the like. According to a method readily carried out by a person with a vision, it can be prepared in unit dose form or by incorporation into a multi-dose container by formulating with a pharmaceutically acceptable carrier and / or excipient. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or aqueous medium, or may be in the form of extracts, powders, granules, tablets or capsules, and may further include a dispersing or stabilizing agent. The 'inhibition of inflammation' of the present invention, like the anti-inflammatory described above, inhibits the cellular cellular inflammation response such as infiltration of immune cells, secretion of proinflammatory factors such as proinflammatory cytokines, prostaglandins and nitric oxide (NO), Erythema, keratinization, skin thickness increase ᅳ Prevents, alleviates or improves symptoms such as stratification, fever and pain. The term 'compr i sing' of the present invention is used in the same way as 'containing' or 'characteristic', and any additional ingredient element or method step not mentioned in the composition or method, etc. Does not exclude The term 'consi sting of' means to exclude additional elements, steps or components, etc., unless otherwise specified. The term 'essent i al ly cons i sting of' refers to a component element or step that is described in addition to a component element or step that does not substantially affect its basic properties, and the like. It means to include.

[Favorable effect]

Therefore, the present invention is an alfalfa extract and kohlrabi extract and additionally broccoli extract, sistane tubulosa extract and kelp extract further comprises one or more extracts selected from the group for hair growth promoting and anti-inflammatory food compositions and cosmetics To provide a composition. The composition of the present invention has the effect of increasing the activity and / or expression of factors that promote hair growth in the skin to create a skin environment to promote hair growth, and excellent effect of inhibiting skin inflammation that can cause hair loss and dandruff There is. 【Brief Description of Drawings】

 Figure 1 shows the activity of γ-glutamyl transpept idase measured in the skin of mice treated with alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a mixture for 14 days. Indicates. * Indicates statistically significant difference? <0.05) compared to the control group (Normal). Figure 2 shows the activity of alkal ine phosphatase measured in the mouse skin administered alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination for 14 days. * Indicates statistically significant difference ^ 0.05) compared to the control (Normal). Figure 3 shows the expression level of EGF measured in the mouse skin administered alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination for 14 days. * Indicates statistically significant difference (/<0.05) compared to control (Normal).

 Figure 4 shows the expression level of VEGF measured in the skin of mice treated with alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination for 14 days. * Indicates a statistically significant difference τ <0.05) compared to the control (Normal).

 Fig. 5 shows the concentration of N0 measured in RAW 264.7 cell cultures treated with alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or mixture and IFN Y and LPS stimulated. Indicates. * Indicates a statistically significant difference (0.05) compared to the control group, # compared with the positive control group (IFN—γ + LPS).

 Figure 6 shows the amount of exudate produced by inflammation caused in the skin of the mouse administered alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination Indicates. * Indicates a statistically significant difference (FB05) compared to a normal control (#) compared to a normal control (carageenan).

7 is measured in the exudate produced by inflammation caused in the skin of the mouse administered alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination The concentration of N0 is shown. * Is statistically compared with normal compared with #, with carageenan positive. Significant difference (<0 · 05) is indicated.

Figure 8 is measured in the exudate produced by inflammation caused in the skin of the mouse alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or a combination The concentration of PGE ₂ is shown. * Indicates a statistically significant difference (^ 0.05) when compared with a normal control (#) compared with a positive control (carageenan).

[Form for implementation of invention]

 Hereinafter, the present invention will be described in detail.

 However, the following examples are merely to illustrate the present invention, the content of the present invention is not limited to the following embodiments.

Experimental Method

 Extract manufacturer

In this example, alfalfa, kohlrabi and broccoli were used for each shoot. Alfalfa sprout extract and kohlrabi sprout extract were sterilized by solvent extraction with 70% ethanol aqueous solution, concentrated and dried to prepare a powder. Broccoli sprout extract was prepared by washing the broccoli sprout, dried and pulverized, solvent extraction with 70% ethanol aqueous solution at 40 ^° C, sterilization, concentration and drying. Cystane Tubulosa extract was prepared by disinfection with 80% ethane in an aqueous solution, sterilized and dried. Kelp extract was washed, dried and crushed kelp, extracted with hot water at 60 ^° C for one day, filtered through a membrane of 0.45 μ ηι, sterilized, concentrated and spray dried to prepare a powder. Hair Growth Animal Experiment

Five-week-old male C57BL / 6 mice were supplied from Orient Bio (Gapyeong, Korea) and used for about one week after acclimation. Animals were housed 4 in a mouse cage (220 × 200 × 145 mm 3). The environment of the animal laboratory was controlled at a temperature of 23 ± 2 ° C, a relative humidity of 55 ° C, 10%, a ventilation rate of 12 times / hour, a lighting cycle of 12 hours, and an illuminance of 150-300Lux. Purina Rat Chow, a pel let solid feed for experimental animals, was supplied from Biopia (Gunpo, Korea), and drinking water was freely consumed with sterile purified water. The dorsal area of 6-week-old mice (n = 8 / group) was epilated with an electric razor The hair roots were allowed to be induced into anagen. In other words, wait until the depilated skin has a uniform pink color, and when the pink color is confirmed, the remaining hair is lightly shaved so as not to damage the skin with the razor.

 To the shaved mice, the extracts prepared above were administered orally daily for 14 days, either alone or in combination, and the synergistic effect between the test substance compositions was evaluated. As a positive control, minoxidil (MXD) was applied to the epilation site once daily for 14 days from the day after epilation using a brush. The dosage of the extract alone is as follows (unit is mg mg / kg body weight): alfalfa sprout extract (54 mg / kg); Kolbi Sprout Extract (54mg / kg); Broccoli Sprout Extract (27mg / kg); Cystane tubulosa extract (54 mg / kg as extract 1; 162 mg / kg as extract 2); Doses of kelp extract (18 mg / kg as extract 1; 54 mg / kg as extract 2) / combination extract are as follows: alfalfa and kohlrabi sprout extract combination composition (mouse dose 54 mg / kg each); Alfalfa sprout, kohlrabi sprout and broccoli sprout extract combination composition (alpapa and kohlrabi sprout extract each 54 mg / kg and broccoli sprout extract 27 mg / kg); A composite composition containing all five extracts, including alfalfa sprouts, kohlrabi sprouts, broccoli sprouts, cystane tubulosa and kelp extracts (composition 1, alfalfa and kohlrabi sprout extracts each 18mg / kg, broccoli sprout extracts 9mg / kg, 5½ g / kg of cisantebulus extract and 18 mg / kg of kelp extract; 54 mg / kg of alfalfa and kohlrabi sprout extracts as composition 2, 27 mg / kg of broccoli sprout extract, 162 mg / kg of sistem tubulo extract and 5½ g of kelp extract / kg). Calculation of y -Glutamyl transpeptidase (γ -GTP) and alkaline phosphatase (ALP) activities

 Skin tissues extracted from the mice were immediately frozen in liquid nitrogen, and added with 4 times phosphate buffer (H 7.4) and homogenized. The homogenate was centrifuged at 3,000 rpm for 20 minutes, and the supernatant was used for measuring Y-GTP and ALP activity. Y-GTP and ALP activities were measured by enzyme rate assay (GSCC) using p-nitrophenolphosphate (PNPP) -DEAV.

Epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) expression levels

EGF and VEGF were measured using enzyme-1 inked immunosorbent assay (ELISA) kits (R & D Systems, Minneapolis, USA). In other words, follow the instructions of the weeder The production of two growth factors was quantified by sandwich enzyme immunoassay using polyclonal antibodies specific for EGF and VEGF. Cell culture and NO quantification

To assess the effect of the composite composition on NO production, 画 264.7 cells (lxl0 ⁶ cells / mL) were treated with respective extracts and complex compositions for 5 minutes, followed by interferon-γ (IFN-γ, lOU / mL) and 1 Ipopolysaccharide (LPS, lOyg / mL) was added and incubated for 24 hours. Nitrite (N0 ² ), an oxidation retardant of NO, was measured as an indicator of NO.The culture medium was equally charged with Griess reagent (1% sulfanilamide, 0.1% naph t hy 1 et hy 1 ened i am i de in 2.5% phosphoric acid). After reaction with, the mixture was reacted at room temperature for 10 minutes. The NO content was measured by absorbance at 540 nm and quantified using sodium nitrite (NaN0 ₂ ) standard quantitative curve.

Anti-inflammatory animal experiment

A 7-week-old male ICR mouse was supplied from Daehan Biolink (Voice, Korea) and used for about 1 week after acclimation. Animals were housed 4 in a mouse cage (220 × 200 × 145 mm). The environment of the animal laboratory was controlled at a temperature of 23 ± 2 ^° C, a relative humidity of 55 ± 10%, a number of ventilations per hour / hour, a lighting cycle of 12 hours, and an illuminance of 150-300Lix. Purina Rat Chow, a pellet-type solid feed for experimental animals, was supplied from Biopia (Gunpo, Korea), and negative water was allowed to ingest sterile purified water freely.

 Mice (n = 8 / group) were administered orally with alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane tubulosa extracts, kelp extracts alone, or a combination of these. Black alone was administered in combination and the dose was as in hair growth animal experiments. The purpose of this study was to evaluate the highest synergistic effect among test substances according to the mixing of extracts. An air pouch was made by injecting 10 mL of sterile air into the dorsal subcutaneous area on the start of test substance administration. After 2 and 5 days, 5 mL of air was additionally injected to keep the air bag stable, and 1 mL of carrageenan () in saline; Sigma) or solvent only was injected into the air bag. Exudate Analysis

After 6 hours of Carrageenan injection, the air sacs were washed with lmL cold saline The net volume of the lavage f luid was recorded. Inflammatory mediators NO and PGE ₂

It was measured by enzyme immunoassay (EIA) using Griess reagent (Sigma) and Correlate-EIA kit (Assay Designs, Ann Arbor, Ann Arbor, USA). Statistical processing

 All measurements are expressed as mean standard error. If significance was observed after one-way AN0VA analysis, Turkey's test was used to test the significance between groups at the level of τ 0.05.

<Example 1>

 Effect of Complex Extracts on Hair Growth Example <1-1> γ-Glutamyl transpept idase Activity

The effect of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, or the extract of kelp extract alone or in combination on the activity of γ-glutamyl transpept idase (Y-GTP) was investigated. γ-GTP is an important enzyme for hair growth known to function in the hair follicles to induce hair follicles into anagen. Depending on the experimental conditions, each extract was administered orally to mice alone or in combination for 14 days, and Y-GTP activity in the skin of the mice was measured. As shown in Figure 1, alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract kelp extract, respectively, when treated alone, compared to the control (Normal) tends to increase the _Y GTP activity Indicated. On the other hand, when alfalfa sprout extract and kohlrabi sprout extract are administered together (alfalfa + kohlrabi), and when alfalfa sprout extract, kohlavi sprout extract and broccoli sprout extract are administered together (alfalfa + kohlrabi + broccoli) Compared with the control group, Y GTP activity showed a statistically significant increase. In addition, when a complex extract (composite composition 1 and 2) containing all alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane tubulosa extracts, and kelp extracts, each of the single or alfalfa sprout extracts ， Kolbi sprout extract ， Broccoli sprout extract showed the most excellent effect of increasing the Y GTP activity even compared to the composition. In particular, compared with the existing hair regrowth minoxidil (3%) showed similar levels. The results of the above experiments have the effect of increasing the Y GTP activity in the mixed composition of alfalfa sprout extract and kohlrabi sprout extract, especially broccoli sprout extract, cystane tubulosa extract and kelp extract in alfalfa and kohlrabi sprout extract. If further included, it can be seen that the Y GTP activity is maximized due to the synergistic effect of the combination of the extracts. Example <1-2> Akal ine phosphatase activity measurement

 The effects of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract alone or in combination were investigated on the activity of akal ine phosphatase (ALP). ALP is known as an indicator of skeletal growth and angiogenesis in animals. Depending on the experimental conditions, ALP activity was measured in mouse masses which were orally administered for 14 days with each extract alone or in combination. As shown in Fig. 2, when alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract were treated alone, the ALP activity showed a tendency to increase slightly compared to the control group. It was. On the other hand, when alfalfa sprout extract and kohlrabi sprout extract were administered together (alfalfa + collabi) and broccoli sprout extract was added (alfalfa + collabi + broccoli), ALP activity was statistically significant compared to the control group. Showed an increase. In addition, when a complex extract (composite composition 1 and 2) containing all alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane tubulosa extracts, and kelp extracts, each of the individual extract or alfalfa sprout extracts ， The highest increase in ALP activity was observed compared to the mixed composition of Kolbi sprout extract and broccoli sprout extract, and showed similar effect to the minoxidil (Minoxidil, 3%), an existing hair restorer.

 The results of the above experiments have the effect of increasing ALP activity in the combination composition of alfalfa sprout extract and kohlrabi sprout extract, and especially broccoli sprout extract, cystane tubulosa extract and kelp extract on alfalfa and kohlrabi sprout extract. In addition, it can be seen that the ALP activity is maximized due to the synergistic effect of the combination of the extracts. Example <1-3> Epidermal growth factor expression level measurement

Alfalfa Sprout Extract ， Kolbi Sprout Extract ， Broccoli Sprout Extract ， Sistanche We investigated the effect of TBULUSA extract, kelp extract alone or the combination on the expression level of epidermal growth factor (EGF). EGF is known to promote hair growth. Depending on the experimental conditions, each extract was administered orally to mice alone or in combination for 14 days, and the amount of EGF expression in the skin of the mice was measured. As shown in Figure 3, when the alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone, respectively, compared to the control (Normal), the amount of EGF expression is slightly increased. The trend was shown. On the other hand, if alfalfa sprout extract and kohlrabi sprout extract are administered together (alfalfa + collabi), and alfalfa sprout extract, kohlavi sprout extract and broccoli sprout extract are administered together (alfalfa + collabi + broccoli) In comparison, the expression level of EGF was statistically significant. In addition, when a complex extract (composite composition 1 and 2) containing all alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane tubulosa extracts, and kelp extracts, each of the individual extracts, alfalfa sprout extracts, It showed the most significant EGF expression weighting effect than the mixed composition of non-sprout extract and broccoli sprout extract. In particular, it was observed at a similar level compared to the minoxidil (Minoxidil, 3%) of the existing hair regrowth.

 The results of the above experiments have the effect of increasing the expression level of EGF in the combination composition of alfalfa sprout extract and kohlrabi sprout extract, especially alfalfa and kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract and kelp extract Including, it can be seen that the increase in the amount of EGF expression is maximized due to the synergistic effect of the combination of extracts. Example <1-4> Expression of Vascular endothel ial growth factor expression

The effects of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract alone or in combination were investigated on the expression levels of vascular endothel ial growth factor (VEGF). VEGF is an angiogenesis factor. Depending on the experimental conditions, each extract was administered orally to mice alone or in combination for 14 days, and VEGF expression in the skin of the mice was measured. As shown in Fig. 4, alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract each treated alone Compared with the right control group (Normal), VEGF expression did not change significantly. On the other hand, if alfalfa sprout extract and kohlrabi sprout extract are administered together (alfalfa + collabi), and alfalfa sprout extract, kohlavi sprout extract and broccoli sprout extract are administered together (alfalfa + collabi + broccoli) Compared with the control group, VEGF expression was statistically significant. In addition, a combination of alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane tubulosa extracts, and kelp extracts (composite compositions 1 and 2), when administered alone, alfalfa sprout extracts, cola It showed the most remarkable effect of increasing the expression of VEGF than the mixed composition of non-sprout extract and broccoli sprout extract. In particular, it was observed at a similar level compared to the existing hair growth agent, minoxidil (3%).

 The results of the above experiments have the effect of increasing the expression level of VEGF in the mixed composition of alfalfa sprout extract and kohlrabi sprout extract, and especially broccoli sprout extract, cystane tubulosa extract and kelp extract on alfalfa and kohlrabi sprout extract. Including, it can be seen that the increase of VEGF expression is maximized due to the synergistic effect of the combination of extracts.

<Example 2>

 Effect of Complex Extracts on Inflammatory Reaction Example <2-1> Measurement of NO Production of Macrophages Induced by IFN Y and LPS

Production of NO in macrophages induced by IFN-γ and LPS to investigate the effects of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, kelp extract alone or in combination The effect on the was measured. Depending on the experimental conditions, each extract was treated alone or in combination with RAW 264.7 macrophage line for 5 minutes, and stimulated with IFN-γ (10U / ml) and LPS (10ug / ml) to determine the amount of NO produced. . . As can be seen in Figure 5, alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, sistane tubulosa extract, kelp extract when treated alone ^ compared to the control (IFN- Y + LPS) production There was a tendency to decrease. On the other hand, when alfalfa sprout extract, kohlrabi extract (alfalfa + collabi) or broccoli extract were added, (alfalfa + collabi + broccoli) NO production was further reduced compared to the positive control group. Turned on. On the other hand, when a combination of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cistanche turbulosa extract, and seaweed extract (compositions 1 and 2) were treated, the NO reduction effect was greatest.

 The above results show that the NO production inhibitory effect can be significantly increased by mixing the single extract. Example <2-2> Skin inflammation exudates

 To determine the effects of alfalfa sprout extracts, kohlrabi sprout extracts, broccoli sprout extracts, cystane bulrosa extracts, kelp extracts, or the combinations on the inflammatory response, the amount of effusion produced by the inflammatory response in mice Was measured. Depending on the experimental conditions, each extract was administered orally to mice alone or in combination for 7 days. In order to induce inflammation, sterilized air is injected into the skin such as mice on the 2nd and 5th day of the experiment, and the airbag is filled with carageenan (l%, 1ml) on the last day. The volume of the resulting exudates was measured. As can be seen in Figure 6, alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, sistane tubulosa extract, kelp extract when administered alone, compared to the control (Carageenan) has a significant effect of significantly reducing the production of exudates Was observed. When alfalfa sprout extract and kohlavi extract were administered together (alfalfa + cola ratio) or further treated with broccoli extract (alfalfa + kohlrabi + broccoli), the production of exudates was further reduced. On the other hand, when the alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and the complex extract (compositions 1 and 2) containing all of the kelp extract were treated, the effect of inhibiting the exudate was shown to be greatest. In particular, the exudates of the mice treated with the complex extract were found to be similar to or lower than the normal group.

 The above experimental results show that the synergistic effect of inhibition of exudate production due to inflammation in the body can be obtained by mixing the single extract. Example 2 Measurement of NO Concentration of Skin Inflammation Exudate

To investigate the effects of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract on the inflammatory response Inflammation was induced in the mice, and the amount of NO produced by the reaction reaction was confirmed. According to the experimental conditions obtained in Example <2-2>, the concentration of NO present in the effusion of the air sacs of the mice to which each extract was administered alone or in combination was measured, and each experimental group and the control group were compared. . As can be seen in Figure 7, alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, sistane tubulosa extract, kelp extract when administered alone, compared to the control (Carageenan) has a significantly lowered NO production effect Was observed. When alfalfa sprout extract and kohlavi extract were administered together (alfalfa + bulavi) or broccoli extract added (alfalfa + collabi + broccoli), NO concentration in the exudates was further reduced. On the other hand, when the alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract were all treated, the composition (composition 1 and 2) had the lowest NO concentration in the effluent.

Therefore, it can be seen that the synergistic effect of the inhibition of NO production in the exudate as well as the inhibition of the exudate generation due to inflammation in the body by mixing the extract alone. Example <2-4> Determination of PGE ₂ Concentration in Skin Inflammatory Effusions To determine the effect of alfalfa sprout extract, kohlrabi sprout extract, broccoli sprout extract, cystane tubulosa extract, and kelp extract on inflammatory reaction, Inflammation was induced, and production of? 0¾, another inflammatory mediator, was confirmed. According to the experimental conditions obtained in Example <2-2>, the concentration of PGE ₂ present in the effusion of the air sacs of the mice administered alone or in combination with each extract was measured, and each experimental group and the control group were compared. It was. As can be seen in Figure 8, alfalfa sprout extract, Klavi sprout extract, broccoli sprout extract, sistane tubulosa extract, kelp extract when administered alone, compared to the control (Carageenan) significantly lower the production of PGE ₂ has an effect Was observed. When alfalfa sprout extract and kohlrabi extract (alfalfa + collabi) or broccoli extract were administered together (alfalfa + collabi + broccoli) further reduced PGE ₂ concentration in the effusion. Alfalfa Sprout Extract ， Kolbi Sprout Extract, Broccoli Sprout When the composite composition (compositions 1 and 2) containing all of the extract, cystane tubulosa extract, and kelp extract were observed, the concentration of PGE _{2 in} the effusion was lowest.

The results of the above experiments show that by mixing the extract alone, the synergistic effect of inhibiting the production of inflammatory mediators such as NO, PGE _{2 in the} effusion, as well as the inhibition of the production of effusion due to inflammation in the body can be obtained.

Industrial Applicability

 As described above, the present invention provides a composition comprising alfalfa extract and kohlrabi extract, and further comprising one or more extracts selected from the group consisting of broccoli extract, cystane tublosa extract, and kelp extract. Provided are an anti-inflammatory food composition and a cosmetic composition. The composition of the present invention has the effect of activating a factor that promotes hair growth and increasing expression in the skin, creating a skin environment that promotes hair growth, and having an excellent effect of inhibiting skin inflammation, thus preventing hair loss and dandruff. And it can be useful for developing functional foods and cosmetics to promote hair growth.

Claims

[Range of request]

 [Claim 1]

 Hair growth promoting and anti-inflammatory food composition comprising alfalfa extract and kohlrabi extract.

[Claim 2]

 The composition according to claim 1, wherein the composition comprises alfalfa extract and kohlrabi extract in a weight ratio of 1: 0.1-10.

[Claim 3]

 The composition of claim 1, wherein the composition further comprises one or more extracts selected from the group consisting of broccoli extract, cystane tubulosa extract, and kelp extract.

[Claim 4]

 The composition of claim 3, wherein the composition comprises alfalfa extract, kohlrabi extract, broccoli extract, cystane tubulosa extract, and kelp extract at a weight ratio of 1: 0.1-10: 0.1-5: 0.1-30: 0.1-10 A composition comprising a.

[Claim 5]

 The composition according to any one of claims 1 to 4, wherein the extract is extracted with water, an alcohol having 1 to 6 carbon atoms, any one solvent selected from the group consisting of spirits and mixed solvents thereof. .

[Claim 6]

 Hair growth promoting and anti-inflammatory cosmetic composition comprising alfalfa extract and kohlrabi extract.

[Claim 7]

The composition of claim 6, wherein the composition further comprises one or more extracts selected from the group consisting of broccoli extract, cystane tubulosa extract, and kelp extract.

[Claim 8]

 According to claim 7, wherein the composition is alfalfa extract, kohlrabi extract, broccoli extract, cystane tubulosa extract and kelp extractol 1: 1: 0.1 ~ 10: 0.1-5: 0.1-30: 0.1 to 10 by weight ratio A composition comprising a.

[Claim 9]

 Use of alfalfa and cola extracts for the preparation of agents for promoting hair growth and for anti-inflammatory.

[Claim 10]

 A method for promoting hair growth and inhibiting inflammation comprising administering to a subject in need thereof an effective amount of a composition comprising an alfalfa extract and a kohlrabi extract.