CN109481663B - Composition for treating alopecia and preparation method thereof - Google Patents
Composition for treating alopecia and preparation method thereof Download PDFInfo
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Abstract
A composition for treating alopecia and its preparation method are provided. It relates to a composition, a preparation method and application thereof, and relates to the field of daily chemicals. The invention aims to solve the technical problems that the alopecia phenomenon is increasingly prominent in people and the trend of youth is presented. The composition for treating alopecia mainly comprises earthworm protein cut-off liquid and fermented extract of fermented soybean. Preparation: firstly, preparing earthworm protein cutting liquid; secondly, fermented soya beans are fermented. The composition for treating alopecia has obvious promotion effect on hair follicle growth and obvious improvement effect on seborrheic alopecia.
Description
Technical Field
The invention relates to a composition and a preparation method thereof, and relates to the field of daily chemicals.
Background
With the increasing pace of life and the increasing pressure of life, the phenomenon of alopecia in people is increasingly prominent and younger. Physiological alopecia refers to the condition of hair that is in dynamic balance between the anagen phase and the catagen phase, so that the normal amount of hair is maintained. Pathological hair loss refers to abnormal or excessive loss of hair, and is mostly considered to be related to the following factors: hereditary factors, excessive secretion of male hormone, mental stress, emotional instability, sleep deficiency, microelement deficiency, stress hypofunction, autoimmune endocrine function disorder, menoxenia and puerperal dysregulation, middle-aged kidney deficiency, etc. These factors cause deterioration of head ecological environment such as unbalance of scalp nutrition supply, poor blood circulation, excessive fat, hair follicle obstruction, etc., thereby preventing nutrient absorption by hair roots, affecting hair growth, and causing alopecia.
Disclosure of Invention
The invention aims to solve the technical problems that the alopecia phenomenon is increasingly prominent in people and the trend of youth is shown, and provides a composition for treating alopecia, a preparation method and application thereof.
The composition for treating alopecia mainly comprises earthworm protein cut-off liquid and fermented extract of fermented soybean.
The composition for treating alopecia further comprises a preservative, a humectant, a nutrition conditioner and a pH regulator;
the preservative accounts for 0.3 to 0.6 percent of the total mass of the composition for treating alopecia, the humectant accounts for 20 to 30 percent of the total mass of the composition for treating alopecia, the nutrition conditioner accounts for 40 to 50 percent of the total mass of the composition for treating alopecia, and the pH regulator accounts for 0.2 to 0.3 percent of the total mass of the composition for treating alopecia.
The preservative is methyl paraben, benzoic acid, benzyl alcohol or potassium sorbate;
the humectant is glycerol, propylene glycol, polyethylene glycol or sorbitol;
the pH regulator is sodium hydroxide or potassium hydroxide;
the nutrition conditioner is one or a mixture of more of higher alcohol, white oil, cetyl alcohol and polyether.
The preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, regulating pH value to 6.5-6.7, centrifuging at 20-30 deg.c in a tubular centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of the composite solution of the bacillus subtilis with the concentration of 107-109CFU/ml and the earthworm protein cut-off solution into every 250g of the fermentation substrate, shaking up, and culturing for 72-120 hours at 4-8 ℃ to obtain the earthworm protein cut-off solution and fermented black bean extract.
And the centrifugal pressure in the second step is 0.25 MPa.
And in the second step, the centrifugal rotating speed is 200 r/min.
The preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, regulating pH value to 6.5-6.7, centrifuging at 20-30 deg.c in a tubular centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of a composite solution of the bacillus subtilis with the concentration of 107-;
fourthly, adding a preservative, a humectant, a nutrition conditioner and a pH regulator into the earthworm protein cut-off liquid and fermented black bean extract to obtain the composition for treating alopecia
The application of the composition for treating alopecia is to prepare a medicament, a shampoo and a scalp massage cream for treating alopecia by adopting the composition for treating alopecia.
The scalp massage cream comprises 15g of stearic acid, 8g of glyceryl monostearate, 8g of white oil, 8g of liquid paraffin, 5g of vaseline, 5g of cetyl alcohol, 0.3g of sodium hydroxide, 30ml of earthworm protein cut liquid and fermented black bean extract, 20ml of propylene glycol, 10ml of astragalus extract and 100ml of distilled water.
Lumbricus, also known as earthworm, has the actions of clearing heat and calming wind, relieving asthma, dredging collaterals, and inducing diuresis. Modern pharmacological research finds that the traditional Chinese medicine composition has the effects of anticoagulation, thrombolysis, platelet aggregation inhibition, arrhythmia resistance, cancer resistance, pain relief, fever relief and asthma relief.
Earthworm contains protein with large specific gravity and abundant varieties, accounting for 56% -65% of dry weight, lipid protein, antimicrobial protein, vasoconstriction protein, hemolysis and coagulation protein, calmodulin binding protein, earthworm new calcium binding protein, etc.
The fermented soya beans contain rich nutrient substances, mainly including protein, free amino acid, essential fatty acid, soluble sugar, inorganic salt, vitamins and the like. In addition, semen Sojae Preparatum also contains physiologically active substances such as soybean isoflavone, soyasaponin, brown pigment, semen Sojae Preparatum plasmin, beta-glucosidase, and antibiotics. The active substances in the fermented soybean are partially derived from raw material soybean, and partially derived from various microorganisms and enzymes involved in the fermentation process to carry out complex biochemical reactions such as decomposition, recombination and the like on organic matters in the raw material soybean.
The composition for treating alopecia has obvious promotion effect on hair follicle growth and obvious improvement effect on seborrheic alopecia.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the composition for treating alopecia of the present embodiment mainly comprises a earthworm protein cut-off solution and a fermented extract of fermented soybean.
The second embodiment is as follows: this embodiment differs from the first embodiment in that the composition for treating alopecia further comprises a preservative, a humectant, a nutritional conditioner, and a pH adjuster;
the preservative accounts for 0.3 to 0.6 percent of the total mass of the alopecia treating composition, the humectant accounts for 20 to 30 percent of the total mass of the alopecia treating composition, the nutrition conditioner accounts for 40 to 50 percent of the total mass of the alopecia treating composition, and the pH regulator accounts for 0.2 to 0.3 percent of the total mass of the alopecia treating composition. The rest is the same as the first embodiment.
The third concrete implementation mode: this embodiment differs from the first or second embodiment in that the preservative is methylparaben, benzoic acid, benzyl alcohol, or potassium sorbate; the humectant is glycerol, propylene glycol, polyethylene glycol or sorbitol; the pH regulator is sodium hydroxide or potassium hydroxide; the nutrition conditioner is one or a mixture of more of higher alcohol, white oil, cetyl alcohol and polyether. The other is the same as in the first or second embodiment.
When the nutritional conditioner is a mixture, the components are in any ratio.
The fourth concrete implementation mode: detailed description of the inventiona method for preparing the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, regulating pH value to 6.5-6.7, centrifuging at 20-30 deg.c in a tubular centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of the composite solution of the bacillus subtilis with the concentration of 107-109CFU/ml and the earthworm protein cut-off solution into every 250g of the fermentation substrate, shaking up, and culturing for 72-120 hours at 4-8 ℃ to obtain the earthworm protein cut-off solution and fermented black bean extract.
The fifth concrete implementation mode: the present embodiment is different from the fourth embodiment in that the centrifugal pressure in the second step is 0.25 MPa. The rest is the same as the fourth embodiment.
The sixth specific implementation mode: the fourth or fifth embodiment is different from the fourth or fifth embodiment in that the centrifugal speed in the second step is 200 r/min. The other is the same as the fourth or fifth embodiment.
The seventh embodiment: the preparation method of the composition for treating alopecia according to the second embodiment comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, regulating pH value to 6.5-6.7, centrifuging at 20-30 deg.c in a tubular centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of a composite solution of the bacillus subtilis with the concentration of 107-;
fourthly, adding a preservative, a humectant, a nutrition conditioner and a pH regulator into the earthworm protein cut-off liquid and fermented black bean extract to obtain the composition for treating alopecia;
the preservative accounts for 0.3 to 0.6 percent of the total mass of the alopecia treating composition, the humectant accounts for 20 to 30 percent of the total mass of the alopecia treating composition, the nutrition conditioner accounts for 40 to 50 percent of the total mass of the alopecia treating composition, and the pH regulator accounts for 0.2 to 0.3 percent of the total mass of the alopecia treating composition;
the preservative is methyl paraben, benzoic acid, benzyl alcohol or potassium sorbate;
the humectant is glycerol, propylene glycol, polyethylene glycol or sorbitol;
the pH regulator is sodium hydroxide or potassium hydroxide;
the nutrition conditioner is one or a mixture of more of higher alcohol, white oil, cetyl alcohol and polyether.
When the nutrition conditioner is a mixture, the components are in any ratio
The specific implementation mode is eight: the composition for treating alopecia according to one of the first to seventh embodiments is used for preparing a medicine for treating alopecia, a shampoo and a scalp massage cream for treating alopecia.
The following examples were used to demonstrate the effect of the invention:
example 1:
fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in water for 10-18 hr, sterilizing at 120 deg.C, and culturing at 4-8 deg.C for 72-120 hr to obtain semen Sojae Preparatum;
the scalp massage cream comprises 15g of stearic acid, 8g of glyceryl monostearate, 8g of white oil, 8g of liquid paraffin, 5g of vaseline, 5g of cetyl alcohol, 0.3g of sodium hydroxide, 30ml of fermented soya beans, 20ml of propylene glycol, 10ml of astragalus extract and 100ml of distilled water.
Example 2:
the preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, adjusting pH to 6.5, centrifuging at 20 deg.C by tube centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of Bacillus subtilis and Lumbricus protein cut-off solution with Bacillus subtilis concentration of 107CFU/ml into 250g of fermentation medium, shaking, and culturing at 4-8 deg.C for 72 hr to obtain Lumbricus protein cut-off solution and fermented semen Sojae Preparatum extract.
The scalp massage cream comprises 15g of stearic acid, 8g of glyceryl monostearate, 8g of white oil, 8g of liquid paraffin, 5g of vaseline, 5g of cetyl alcohol, 0.3g of sodium hydroxide, 30ml of earthworm protein cut-off liquid and fermented black bean extract, 20ml of propylene glycol, 10ml of astragalus extract and 100ml of distilled water.
Example 3:
the preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, adjusting pH to 6.5, centrifuging at 20 deg.C by tube centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 1-4 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of Bacillus subtilis and Lumbricus protein cut-off solution with Bacillus subtilis concentration of 107CFU/ml into 250g of fermentation medium, shaking, and culturing at 4-8 deg.C for 72 hr to obtain Lumbricus protein cut-off solution and fermented semen Sojae Preparatum extract.
The scalp massage cream comprises 15g of stearic acid, 8g of glyceryl monostearate, 8g of white oil, 8g of liquid paraffin, 5g of vaseline, 5g of cetyl alcohol, 0.3g of sodium hydroxide, 30ml of earthworm protein cut-off liquid and fermented black bean extract, 20ml of propylene glycol, 10ml of astragalus extract and 100ml of distilled water.
Example 4:
the preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, adjusting pH to 6.5, centrifuging at 20 deg.C by tube centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 1-5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of Bacillus subtilis and Lumbricus protein cut-off solution with Bacillus subtilis concentration of 107CFU/ml into 250g of fermentation medium, shaking, and culturing at 4-8 deg.C for 72 hr to obtain Lumbricus protein cut-off solution and fermented semen Sojae Preparatum extract.
The scalp massage cream comprises 15g of stearic acid, 8g of glyceryl monostearate, 8g of white oil, 8g of liquid paraffin, 5g of vaseline, 5g of cetyl alcohol, 0.3g of sodium hydroxide, 30ml of earthworm protein cut-off liquid and fermented black bean extract, 20ml of propylene glycol, 10ml of astragalus extract and 100ml of distilled water.
Example 5:
inhibition experiment for seborrheic mouse alopecia
1. Experimental Material
Testosterone propionate injection 25mg/1ml, purchased from Ningbo second hormone factory, production batch number: 11025
Salad oil 90 ml/bottle, Jinlongyu Limited
Adult male B6CBAF1/J mice, 18-22g in weight, clean grade, purchased from beijing vindolizhua experimental animal technology ltd [ license No.: SCXK (Jing) 2017-.
2. Experimental methods
2.1 Experimental groups
10 mice of B6CBAF1/J are randomly selected from 70 male mice as an experimental blank control group, and the other 60 mice of B6CBAF1/J are taken as experimental mice and are raised in cages. Namely, the alopecia model group and the scalp massage cream group (scalp massage creams prepared in examples 1 to 4 were used).
2.2 animal model preparation and administration methods
The hair loss animal model adopts an injection molding mode, the injection part is subcutaneous of the back of the mouse parallel to the spinal area, the injection dose is 5 mg/kg.d, and the injection time is 4 weeks except the blank control group which is injected with normal saline and the rest 6 groups which are injected with testosterone propionate.
When the B6CBAF1/J mouse alopecia animal model is successfully modeled at 4 weeks, the drug treatment is started, 1ml of scalp massage cream matrix is smeared on the back depilated part of each of the blank control group and the alopecia model group, 1ml of scalp massage cream is respectively smeared on the same depilated part of each of the scalp massage cream groups of examples 1 to 4, and the treatment administration is carried out for four weeks. Observing the hair growth condition of the mouse at the moulting part of the mouse, and counting the moulting weight of the mouse; and calculating the ratio of terminal hair/vellus hair under a microscope in combination with pathological observation, and calculating the average.
3. Statistical analysis of data
SPSS20.0 software is adopted for data processing, the difference between groups is analyzed by adopting one-factor variance, and the significance difference with P less than 0.05 is considered to have statistical significance.
4 results of the experiment
(1) Determination of dehairing weight
As can be seen from Table 1: compared with a blank control group, the depilation phenomenon of other experimental mice occurs to different degrees, and the significant difference (P <0.01) exists in other groups except the significant difference of example 2 (P < 0.05); compared with the model group, the depilation phenomenon of the mice in the groups of examples 2 and 4 is improved, wherein the depilation phenomenon of the mice in the group of example 2 is improved obviously (P <0.01) compared with the model group, and the depilation phenomenon of the mice in other groups of examples has no significant difference and has no improvement trend on alopecia.
(2) Determination of the Final Hair/vellus Hair ratio in mice
As can be seen from table 2, the terminal hair/vellus hair ratio of the model group was significantly decreased (P <0.01) compared to the blank control group; examples 1, 3 did not change the terminal hair/vellus hair ratio compared to the model group, and examples 2, 4 both increased the terminal hair/vellus hair ratio, with the improvement effect of example 2 being more pronounced (P < 0.01).
(3) The scalp massage cream of the invention, example 2 (earthworm (50000) + fermented soya beans), has a significant improvement effect on seborrheic alopecia, and example 4 also has an improvement effect, but the effect is not as significant as that of example 2.
TABLE 1 mouse depilation weight determination results
P <0.05 compared to placebo, p <0.01 compared to placebo;
Δ <0.05 compared to model group, and Δ <0.01 compared to model group.
TABLE 2 determination of the ratio of terminal hair/vellus hair in mice
P <0.05 compared to placebo, p <0.01 compared to placebo;
Δ <0.05 compared to model group, and Δ <0.01 compared to model group.
Experimental example 6: influence of scalp massage cream on hair follicle number of mice in anagen phase
1. Experimental Material
Rosin was purchased from Shenzhen Jitian chemical Co., Ltd.; paraffin wax was purchased from (opav glue wax products); adult female C57BL mice, weighing 18-22g, clean grade, were selected as pink skin and the hair was of resting age. Purchased from beijing weitonglihua experimental animal technology ltd [ license number: SCXK (Jing) 2017-0149 ], with a room temperature of 23 ℃, a humidity of 50-60%, ventilation for 2 times per day, each time lasting 1 hour, and free movement, drinking and eating of mice according to their natural life pattern.
2. Experimental methods
2.1 Experimental groups
60 female C57BL mice were randomly divided into 6 groups, negative control group, scalp massage cream group examples 1-4, and were housed in cages, and the negative control group was coated with a blank medium.
2.2 animal model preparation and administration methods
Mixing rosin and paraffin wax 1:1, heating to melt, uniformly coating on the back of a mouse, wherein the coating area is about 2cm multiplied by 3cm, cooling to solidify, and then removing the hair to remove back hair (the growth period of the hair is confirmed to be in the resting period). The next day after depilation, topical skin is directly applied with cotton swab for administration, and the administration is performed 1 time per day with dosage of 1ml each time, and is continuously performed for 20 days. On day 20, the same location of skin tissue blocks on the backs of each group of mice were taken, fixed with 10% formalin, paraffin embedded sections approximately 4-5 microns thick, HE stained, high power field (× 400) number of hair follicles averaged, and statistically processed.
3. Statistical analysis of data
SPSS20.0 software is adopted for data processing, the difference between groups is analyzed by adopting one-factor variance, and the significance difference with P less than 0.05 is considered to have statistical significance.
4 results of the experiment
(1) Effect of Hair follicle Density
The number of hair follicles in each group of mice on day 20 of the experiment is shown in Table 3. Examples 1, 3 had no effect on the number of hair follicles in C57BL mice compared to the blank control group (P > 0.05); however, examples 2 and 4 can increase the number of hair follicles in the blank mice (P <0.01), wherein the effect of example 2 is very obvious, and the hair follicles in the C57BL6 mice can be induced to enter the anagen phase, the anagen phase is prolonged, and the catagen phase is delayed, so that the hair follicle growth promoting effect is realized.
(2) The scalp massage cream of the present invention, example 2 (earthworm (50000) + fermented soya beans), had a significant effect on hair follicle growth, and the improvement of example 4 also had an improvement effect, but the effect was not significant as in example 2.
TABLE 3 mouse depilation weight determination results
Comparison with blank control group p<0.05，**Comparison with blank control group P<0.01
Claims (2)
1. A preparation method of a composition for treating alopecia is characterized in that the composition for treating alopecia comprises earthworm protein cut-off liquid and fermented black bean extract;
the preparation method of the composition for treating alopecia comprises the following steps:
weighing Lumbricus, adding secondary distilled water 3 times of Lumbricus, homogenizing to obtain homogenate, adjusting pH to 6.5-6.7, centrifuging at 20-30 deg.C by adding into secondary distilled water centrifuge via tubular mode, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of bacillus subtilis with the concentration of 10 in every 250g of fermentation substrate7-109Mixing CFU/ml composite solution of Bacillus subtilis and Lumbricus protein solution, shaking, and culturing at 4-8 deg.C for 72-120 hr to obtain Lumbricus protein solution and fermented semen Sojae Preparatum extract.
2. The preparation method of the composition for treating alopecia is characterized in that the composition for treating alopecia also comprises a preservative, a humectant, a nutrition conditioner and a pH regulator;
the preservative accounts for 0.3 to 0.6 percent of the total mass of the alopecia treating composition, the humectant accounts for 20 to 30 percent of the total mass of the alopecia treating composition, the nutrition conditioner accounts for 40 to 50 percent of the total mass of the alopecia treating composition, and the pH regulator accounts for 0.2 to 0.3 percent of the total mass of the alopecia treating composition;
the preparation method of the composition for treating alopecia comprises the following steps:
weighing earthworm, adding secondary distilled water, homogenizing for several times, regulating pH value to 6.5-6.7, centrifuging at 20-30 deg.c in a tubular centrifuge, and collecting supernatant;
filtering the homogenate supernate obtained in the step two by adopting an ultrafiltration membrane with the molecular weight cutoff of 5 ten thousand to obtain earthworm protein cut-off liquid;
thirdly, fermented soya beans fermentation:
cleaning semen glycines, draining, soaking in the solution of Lumbricus protein for 10-18 hr, and sterilizing at 120 deg.C to obtain fermentation medium;
inoculating 2ml of bacillus subtilis with the concentration of 10 in every 250g of fermentation substrate7-109Mixing CFU/ml composite solution of Bacillus subtilis and Lumbricus protein solution, shaking, and culturing at 4-8 deg.C for 72-120 hr to obtain Lumbricus protein solution and fermented semen Sojae Preparatum extract;
fourthly, adding a preservative, a humectant, a nutrition conditioner and a pH regulator into the earthworm protein cut-off liquid and fermented black bean extract to obtain the composition for treating alopecia;
the preservative is methyl paraben, benzoic acid, benzyl alcohol or potassium sorbate;
the humectant is glycerol, propylene glycol, polyethylene glycol or sorbitol;
the pH regulator is sodium hydroxide or potassium hydroxide;
the nutrition conditioner is one or a mixture of more of higher alcohol, white oil, cetyl alcohol and polyether.
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| KR20090076671A (en) * | 2008-01-09 | 2009-07-13 | 홉킨스바이오연구센터(주) | Method of preparing compositions for preventing depilation and for promoting hair-growing |
| CN106138708A (en) * | 2016-08-02 | 2016-11-23 | 苏州莱奥生物技术有限公司 | A kind of Anti-hair loss Chinese medicine nursing liquid |
| CN104739956B (en) * | 2015-02-27 | 2018-01-30 | 武汉健民药业集团股份有限公司 | A kind of purposes of Chinese medicine composition in antiandrogen medicine is prepared |
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| KR20090076671A (en) * | 2008-01-09 | 2009-07-13 | 홉킨스바이오연구센터(주) | Method of preparing compositions for preventing depilation and for promoting hair-growing |
| CN104739956B (en) * | 2015-02-27 | 2018-01-30 | 武汉健民药业集团股份有限公司 | A kind of purposes of Chinese medicine composition in antiandrogen medicine is prepared |
| CN106138708A (en) * | 2016-08-02 | 2016-11-23 | 苏州莱奥生物技术有限公司 | A kind of Anti-hair loss Chinese medicine nursing liquid |
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