KR20090076671A - Method of preparing compositions for preventing depilation and for promoting hair-growing - Google Patents

Abstract

A method for manufacturing a composition for preventing hair loss and promoting hair growth is provided to basically resolve the cause of hair loss without side effect and remove unbalance of hormones. A method for manufacturing a composition for preventing hair loss and promoting hair growth comprises a step of extracting one kind selected from Epimedium sagittatum, Trichosanthes kirilowii, Dalbergia odorifera, Geum japonicum and Uncaria rhynchophylla; a step of extracting polygoni multiflori Radix, black soybean, lycii Fructus, glycyrrhizae radix with low alcohol at 40-90°C or using heat at 85-110°C; a step of mixing the extracts in a weight ratio of 1:1-3; a step of resolving with water or ethylalcohol; and a step of resolving adjuvant ingredient.

Description

Method for producing a composition for preventing hair loss and promoting hair growth {METHOD OF PREPARING COMPOSITIONS FOR PREVENTING DEPILATION AND FOR PROMOTING HAIR-GROWING}

The present invention relates to epimedium sagittatum, Trichosanthes kirilowii, Dalbergia odorifera, Geum japonicum, and Uncaria rhynchophylla, which are oxidative phospholipids. Preparation of a single or complex composition of 1-Palmitoyl-2-Arachidonoyl- sn -Glycero-3-Phosphocholine, OxPAPC), and a composition for preventing hair loss and promoting hair growth, including sewage extract, black bean extract, wolfberry extract and licorice extract It is about a method. In particular, it affects the capillary production of hair root cells, thereby preventing the progression of hair follicle cells' disappearance or atrophy, and has a hair growth effect that makes young hair grow well with new production of hair. It relates to a method for producing a composition for preventing hair loss and promoting hair growth, characterized in that the thickening and durable wool effect.

There are about 100,000 to 150,000 human hairs, and each hair has a different cycle and grows and falls out through the anagen, the catagen, and the telogen. This cycle is repeated over three to six years, with the result that on average 50-100 hairs fall out normally. Generally, the term alopecia refers to the increase in the number of hairs that fall abnormally due to shortening of the hair growth rate in the growth phase and more hair in the degenerative or resting phase. The cause of hair loss is a genetic cause largely, and not only men's problems but also women's hair loss has emerged as a serious modern problem. In addition to genetic factors, hormone imbalance, mental stress in social life, rapid air pollution in modern civilization, ingestion of processed foods, severe illness due to high fever, nutritional imbalances, anticancer and anti-thyroid drugs, oral contraceptives, Various environments such as hair washing habits, shampoos, hair growth walls and the like are known as the main culprit, and various hair loss prevention and hair growth agents have been developed according to these causes. In addition, pharmaceutical companies and companies that make household chemicals are releasing hair loss prevention products one after another, and more natural-friendly products are increasing than chemical products.

Human skin has the effect of converting testosterone, which accounts for most of the male hormone, into dihydrotestosterone (DHT). The change from skin to DHT causes hirsutism as the enzyme's ability to inhibit the process grows. Propecia, sold by MSD, is a hair restorer developed on the principle of inhibiting the conversion to DHT. However, these products have disadvantages such as lowered erectile ability and decreased libido for long-term treatment. Minoxidil, which has been approved by the FDA along with Propecia, is known to have almost no such side effects. Hyundai Mininsil, Hanmi Pharm, Moxidyl, and Sino-Pharma Pharm Boldmin have been developed and marketed with Minoxidil as an external medicine for general medicine.

It is important to minimize the side effects as hair loss treatment requires long-term treatment, and companies are trying to develop products with excellent hair growth effects by taking herbal medicine and natural foods as main ingredients. Adding. Mori Gana Co., Ltd. has developed Mori Gana with minimal side effects with ingredients extracted from black beans, black sesame seeds, walnuts, seaweed and kelp.Pacific Pharmaceuticals has main products consisting of corn oil extract, tocopherol acetate, soluble cystine and ginseng extract. Dr. Mo was developed, and Spela 707, which is sold by Handok Cosmetics, was developed based on herbal extracts such as passersby, Doin, Angelica, Ginseng, and Cordyceps sinensis. In addition, ginseng of Gyeongin Pharm., A mother-grown spring of Chosun Unco., Etc., mainly composed of apricot seed, pearl powder, and tocopherol, has been developed and marketed as a hair loss prevention and hair regrowth agent using natural extract as a raw material. In addition, LG H & H has developed mo and moa containing as its main ingredient: benzyl nicotinic acid, which promotes blood circulation of the scalp, ginseng extracts that regulate male hormone action, right ear extracts, and hinokithiol, a wool component that activates hair follicle cells. CJ Co., Ltd. has developed and marketed the hair-weaving ability that contains PDG (pentadecanoic acid glyceride) as a main ingredient.

To date, many types of hair repellent-related patents have been published or registered in Korea, and related patent contents are as follows. Republic of Korea Patent No. 10-0775668 relates to pine needles, sewage, Angelica, black sesame, raw green, baekhyeop, Sukjihwang, black soybean tea, kelp, seaweed and spinach extract composition, Republic of Korea Patent No. 10-0761660 is sewage, baekyeop, bone chain , Baekbaekpi, Earl of Peony, Angelica, Hwangjeong, Sukjihwang, kelp, Bokbunja and jujube extract are active ingredients. Republic of Korea Patent No. 10-0668878 is red ginseng, red pepper, medicinal herbs, Seokchangpo, larvae, baekbaekpi, upper leaf, baekbaek leaf, ginseng, licorice, pine needles, peony, phoenix, fennel, cornus, Uiin, ginger (health), garlic, black bean, black sesame Its main ingredient is spinach, carrots, kelp, seaweed and peppermint extract. In addition, there is a hair regrowth agent for topical coatings prepared by mixing the extract of mulberry leaf, side white leaf, black sesame, kelp, seaweed of the Republic of Korea Patent No. 10-0476731, a hair regrowth composition containing the natural situation mushroom extract of Republic of Korea Patent No. 10-0348155 And there is a manufacturing method thereof, there is a hair regrowth composition consisting of extracts of Baekdusan, Daesan, gold having a hair follicle bactericidal function of the Republic of Korea Patent No. 10-0487275. However, the above-mentioned conventional technology can be expected to have a slight thickening of hair or healthy hair roots due to nourishment when it is used for a long time, and the effect of sprouting fine hair such as fluffy hair can be expected to effectively prevent hair loss and to promote breakthrough hair growth. Was not. In addition, such a hair loss prevention and hair growth promoting effect is temporary, the use of the material for a long time because the hair immediately falls out and the hair growth phenomenon stops when the use of the material is stopped.

In the world, the FDA-recognized hair regrowth agent consists of two components, minoxidil, an external skin preparation, and propane, an oral skin, and competition for product development is actively taking place. While the development of hair regrowth agents for medicines based on minoxidil and propecia is active in the United States, the development of products based on natural products is being actively conducted. In Japan, which is the most active market development in the global hair growth market, after the successful success of the minoxidil ingredient RiUP, Gao's Sutce is a medicinal hair active substance and pepper sprouts extract. It was developed as a complex prescription product, and Kanebo's medicinal auto-developing Z was developed based on cloves and Eugenol derivatives, and Shiseido's medicinal bulliolim was developed mainly on painting trees and Japanese tea, Yamacha. In addition, the development of products of natural products derived hair regrowth was also active. The hair growth market is expected to expand further, with various types of products such as pharmaceuticals, quasi-drugs, cosmetics, and health foods, which are expected to expand further. However, even if such chemicals and natural products are available on the market, there is still no development of excellent formulations with certain efficacy. The domestic hair loss population is estimated at 3.5 million, or 23%, based on men over 20 years old.Including women and pediatric hair loss populations, there is a much greater need for high-efficiency preparations. Can be.

Therefore, the inventors have conducted experiments on various natural extracts with a more scientific approach to natural compositions, and as a result, a single natural or complex composition of oxidized phospholipids and / or oxidized phospholipids is excellent for hair growth. It was confirmed that the function.

Angiogenesis is a requirement that must be involved in the wound healing process, which is essential for the rejuvenation of wounded tissue. In the early stages of the wound, cells die and an inflammatory reaction occurs due to the destruction of blood vessels. After this inflammatory reaction, devascularization of blood components, activation of platelets, blood coagulation, biological media such as kallikrein, thrombin, plasmin, etc. We go through a series of processes called the formation of. Eventually, new tissue is formed at the wounded site, and the synthesis and reconstitution of the extracellular matrix occurs, so that the wounded tissue is healed to function again. Looking at the process of inflammation, it causes a complex reaction by endothelial cells or immune-related granulocytes, mast cells and platelets, and performs a defense mechanism that inhibits foreign substance penetration into the wound site. In addition to these immunological defense mechanisms, epidermal cells, macrophages, endothelial cells, fibroblasts, etc. secrete a variety of growth factors to promote cell division for the formation of new tissue, and at the same time epidermal cells, endothelial cells, The generation of cell matrix by the matrix proteins of various cells secreted by fibroblasts, etc., completes the healing of wounds and forms new tissues at the wound site. An important phenomenon associated with this complex wound healing response is the formation of blood vessels. Angiogenesis forms new tissues during wound healing, supplying them with appropriate oxygen and nutrients, allowing the tissues to regenerate and function as part of the normal body.

The angiogenesis process results in the release of fibroblast growth factor (FGF) from wounded cells to stimulate vascular endothelial cells in the walls of blood vessels around the wound to release plasminogen activating factor and procollagenase. Begins. Plasminogen activator activates plasminogen as prasmin and procollagenase as collagenase, and these two enzymes break down the basement membrane surrounding the stimulated vascular endothelial cells. Vascular endothelial cell inducers such as heparin or fibronectin fragments leaked from the wound stimulate the vascular endothelial cells to provoke the gastric limbs and move through the breakdown of the basement membrane to connective tissue. After migration, the basement membrane is resynthesized to form a stable structure in the new dermis. Once the capillaries extend from existing blood vessels, lumens are formed and blood can flow. Continual division of vascular endothelial cells results in the expansion of capillaries. The tissues that are damaged by the supply of nutrients by the newly formed capillaries and by various factors are restored to normal tissues through reorganization.

Oxidized phospholipid (OxPAPC) has been shown to promote endothelial cell formation and to enhance capillary production in mice. Oxidative phospholipids also affected angiogenesis by increasing the expression of vascular endothelial growth factors (VEGFs), IL-8 and COX-2, which are neovascularization promoters (Valery N. et al., Circulation Research). 99 (2006) 900-90). On the basis of these results, oxidative phospholipid was used as a substance for promoting blood vessel generation of hair follicle cells, or a single or a combination of whole leaf ulcers, fly, Kangjinhyang, snake radish and light bulbs, which promote the production of neovascularization, was added. Anterior lobe sulcus, fly leaf, and gangjinhyang showed angiogenic activity in CAM assay and in vitro bovine aortic endothelial cell culture (S. Wang et al., Life Sciences 74). (2004) 2467-2478). Rosaceae snakes have been reported to promote angiogenesis in the regeneration of myocardial infarction (Clinical Chemistry 52 (2006) 1460-1468). In addition, Jo, et al. Showed the effect of promoting blood vessel production both in vitro and in vivo. The gene and protein expression levels of VEGF and bFGF (Basic Fibroblast Growth Factor) increased according to the concentrations of the bulbs, which were confirmed by in vivo Matrigel assay (Biol. Pharm. Bull. 28 (12). (2005) 2248-2252).

Sewage, one of the constituents of the mixed extract, is known to have black hair when taken from ancient times, and has a tonic action and energetic enhancement effect. Lump roots are collected in October-November and contain anthraquinone derivatives, mainly chrysophanol and emodin, followed by lane and physcion, and lecithin. It contains the back, which nourishes the hair roots and promotes melanin production, making the hair blacker. It also helps to grow hair well and combs hair well, leaving it black and lustrous. Sewage is good for blood donation and helps to protect the liver and kidneys, so taking it smoothes the face, makes the skin elastic and shiny.

Goji are mature fruits of goji or movie goji, sweet taste, and flat nature. Betaine, polysaccharides, fats, proteins, thiamine, riboflavin, nicotinic acid, vitamin C and trace elements such as potassium, iron, phosphorus, copper, zinc, etc. are suitable for hair products. The addition of goji berry extract to the hair regrowth prevents and treats hair loss, and rapidly produces melanin pigment, making the hair black and shiny. Therefore, due to the lack of trace elements necessary for the human body is excellent in treating the symptoms of turning yellow or white. It is also effective for exfoliation and protects the skin from UV rays and harmful oxygen from the skin. Therefore, when used in cosmetics, the skin is softened, moisturized and nourish the skin.

Licorice is a perennial herb of the dicotyledonous plant Rosaceae, and its main components are glycyrrhizin, glabraic acid, flavonoids and chaponaretin. Licorice has functions such as anti-inflammatory, tissue repair, sebum control, detoxification, and weakening (neutralizing agent), especially keeps the scalp healthy. Root and rhizome of legume licorice, sweet taste, flat nature. Glycyrrazine is a 2-glucuronide of glycyrrhexane (glycyrrhetic acid) and is a sweet component of licorice. Licorice extract has a function of inhibiting the action of tyrosinase and has an effect of interfering with melanin synthesis, and as a pigment remover, it has been found to be much more effective than kojic acid or vitamin C. Licorice is a natural antibiotic that removes detoxification and inflammation. It is good for acne and eczema. It has cell regeneration, anti-inflammatory and sedative effects, so it does not fall into the raw materials of functional herbal cosmetics. It is also used for high quality hair and soothes, protects and nourishes the skin. It neutralizes and removes or lowers toxic substances in cosmetics and harmonizes with all drugs. In addition, it relaxes the muscles and stabilizes the nerves.

Black bean (black soybean) is a perennial perennial of legumes, generally known to promote blood circulation, detoxification, and lipolysis. Isoflavin among the components of the black bean to regulate the male hormone to suppress the cause of hair loss, in particular, it is effective for alopecia areata.

The present invention is a hair follicle containing capillary production promoting material of hair follicle cells, anterior lobe sulcus, fly, Kang Jin-hyang, snake radish and light bulb, single or complex composition of oxidized phospholipid (OxPAPC), and sewage, wolfberry, black bean, licorice It provides a hair growth agent composition having a blood vessel production of cells, excellent hair loss prevention, hair growth effect, and a method for preparing a hair external composition for hair growth.

In order to solve the above problems, according to a preferred embodiment of the present invention, (A) at least one selected from the group consisting of anterior lobe erosion, sky flies, Kangjinhyang, snake radish, and the like, and (B) sewage, black beans, wolfberry and Licorice extracting alcohol using lower alcohol at 40 to 90 ° C. or hot water extraction at 85 to 110 ° C .; Mixing the (A) and (B) extracts so that the weight ratio is 1: 1 to 3; Dissolving the mixture in water or ethyl alcohol to prepare a mixed solution; And it provides a method for producing a composition for preventing hair loss and hair growth comprising the step of further dissolving the auxiliary component in the mixed solution.

According to another suitable embodiment of the present invention, there is provided a method for producing a composition for preventing hair loss and promoting hair growth, further comprising the step of dissolving phospholipid in the mixed solution.

According to another suitable embodiment of the present invention, the adjunct ingredient is a blood circulation improving agent consisting of vitamin, no substrate, gamma linolenic acid, EPA, tocopherol and mixtures thereof; Scalp penetrants consisting of dimethyl sulfoxide, Triton-X and povidone; Glycerin, a skin moisturizer; And it provides a method for producing a composition for preventing hair loss and hair growth, characterized in that the skin stimulant consisting of menthol and peppermint.

According to another suitable embodiment of the present invention, the auxiliary component provides a method for producing a composition for preventing hair loss and promoting hair growth, characterized in that the surfactant, pH adjuster and thickener.

According to another suitable embodiment of the present invention, the composition of the present invention may be provided in the form of a spray, a coating, an ointment, a cream or a shampoo.

Oxygen phospholipid (OxPAPC), whole leaf ulcer, sky fever, kangjinhyang, snake radish, crude composition, etc., the composition of the present invention containing sewage, black soybean, wolfberry, licorice has no side effects, It fundamentally eliminates the cause of hair loss and smoothes the scalp's blood circulation, eliminating the imbalance of hormones, resulting in excellent hair loss prevention and hair production.

In order to achieve the above object, the present invention is excellent angiogenesis, containing essentially single or complex composition of oxidized phospholipid (OxPAPC) and sewage, wolfberry, black soybean, licorice It provides a method for preparing a composition for preventing hair loss and hair growth having a hair loss prevention, hair growth effect.

The method for producing a composition for preventing hair loss and promoting hair growth of the present invention includes: (A) at least one member selected from the group consisting of anterior lobe erosion, flyweed, gangjinhyang, snake radish, and crude composition; And (B) extracting sewage, black beans, wolfberry and licorice using 40 alcohol at 40 to 90 ° C. or hot water extraction at 85 to 110 ° C .; Mixing the (A) and (B) extracts so that the weight ratio is 1: 1 to 3; Dissolving the mixture in water or ethyl alcohol to prepare a mixed solution; And further dissolving an auxiliary component in the mixed solution. In addition, the phospholipid may be dissolved in the mixed solution.

In the mixed content ratio of the extracts (A) and (B), when the ratio is less than 1: 1, the pharmacological effect of the component is not sufficiently obtained, and when it is used in excess of 1: 3, The action is so strong that rather side effects may occur. Therefore, the composition is preferably prepared by mixing in a ratio of 1: 1 to 3, and more preferably, by mixing in a ratio of 1: 1-2.

Specifically, the composition of the present invention is 1 to 15% by weight of phospholipid (OxPAPC), 5 ~ 20% by weight of foliar erosion, 5 ~ 20% by weight, 5 ~ 20% by weight of Kangjinhyang, 5 ~ 20% of radish It may have a composition ratio of 5 to 20% by weight of a single or a composite composition, such as weight%, light bulbs, and 10 to 15% by weight of sewage, 6 to 10% by weight of black beans, 8 to 15% by weight of wolfberry, and 8 to 15% by weight of licorice.

The natural product mixed extract of the present invention is characterized in that the alcohol extract or hot water extract of at least one selected from sesame leaves, sky flies, kangjinhyang, snake radish and crude composition and sewage, wolfberry, black beans, licorice. Alcohol extract is more preferable because alcohol extraction selectively extracts the active ingredient, while hot water extraction reduces the efficacy of unit gram sugar by extracting not only the active ingredient but also non-active ingredients such as lignin.

In another embodiment, the present invention provides hair growth comprising sewage, wolfberry, black soybean, and licorice as a main ingredient in a single or complex composition of anterior lobe vulva, flyweed, kangjinhyang, bamboo radish, and crude phospholipid and / or oxidized phospholipid. It relates to a facilitation composition. Supplementary ingredients are vitamins, minerals, blood circulation improvers, scalp penetrants, skin irritants and / or moisturizers.

First, vitamins such as vitamins B1, B2, B6, B12, C, calcium pantothenate, nicotinic acid amide, folic acid and biotin, and minerals such as zinc, iron, calcium, and magnesium may be added. The use of such vitamins and minerals is preferred because it can enhance the bioactive effect. At this time, the vitamin and minerals are preferably contained in 0.1 to 10% by weight relative to the total weight of the final composition, in particular vitamins B1, B2 and C 0.01 to 3% by weight, 0.01 to 3% by weight relative to the total weight of the final composition And 0.01 to 4% by weight.

Blood circulation improving agent is added to improve blood circulation and to enhance the biological activity effect, gamma linolenic acid, EPA (eicosapentaenoic acid), tocopherol and the like can be used. At this time, the blood circulation improving agent is preferably included in 0.1 to 0.5% by weight relative to the total weight of the final composition.

Scalp penetrants use dimethyl sulfoxide (DMSO), Triton-X or povidone, and are used to enhance the penetration of pharmaceutical components into the dermal layer deep into the scalp. In particular, the addition of menthol, a skin stimulant, increases skin irritation, which further enhances the effect of the pharmaceutical ingredient on the dermal layer of the skin. At this time, the scalp penetrants such as dimethyl sulfoxide, Triton-X, povidone, etc. are each contained in an amount of 1 to 20% by weight based on the total weight of the final composition, and skin irritants such as menthol are included in 1 to 5% by weight of the total weight of the final composition. Preferred, especially when the scalp penetrants dimethyl sulfoxide, Triton-X and povidone are used separately, 1.0 to 20% by weight of dimethyl sulfoxide, 1.0 to 10.0% by weight of triton-X and povidone relative to the total weight of the final composition It is preferably included in 1.0 to 5.0% by weight. In particular, the old scalp layer, such as bald head is difficult to penetrate the usual drug ingredients, many kinds of hair growth, hair growth agent is also a major cause that does not exhibit its efficacy. Among them, dimethyl sulfoxide (DMSO) is preferable as a scalp penetrant, and DMSO is a low molecular compound, which has a property of passing through a biological membrane well and increasing cell membrane permeability of other substances. DMSO also has an antioxidant action that removes hydroxy radicals (OH '). DMSO has the advantage of dissolving water, alcohol and almost all organic and biochemicals, and is widely used as a solvent for biopolymers in nuclear magnetic resonance analyzers. The mechanism by which DMSO helps infiltrate the scalp has not been elucidated, but it is believed to be due to the ability to instantly dissolve polymers such as proteins with excellent dissolving power.

Moisturizers are added to increase the moisturizing effect of the final composition to enhance the sustainability of the drug, with glycerin being preferred and added at 0.5 to 5% by weight relative to the total weight of the final composition.

In addition, the hair growth promoting composition of the present invention includes extracts of sewage, wolfberry, and the like, and solvents for dissolving all the various components such as various vitamins, minerals, and scalp penetrants, and such solvents may be water or ethyl alcohol. have.

Hereinafter, an alcohol extraction method will be described. After washing each of the natural medicines cleanly, lower alcohols such as methanol and ethanol, preferably 70-95% ethanol, 3 to 8 times by weight, 40 to 90 ℃, preferably 60 to At 85 ° C., it is heated to reflux for 1 to 48 hours, preferably 2 to 12 hours, and the solids are removed using a filter paper or a conventional filtration apparatus in a hot state without cooling. The filtered filtrate is dried under reduced pressure using a rotary evaporator or a conventional concentrator to prepare the final alcohol extract. Normally, 1.0Kg of natural product is extracted and the decompression process is completed to obtain 400g of concentrated extract. The final alcohol extract thus obtained is dissolved in a solvent such as water or ethyl alcohol to prepare a composition of the present invention.

As a hot water extraction method, after washing each of the natural medicinal herbs cleanly, using the water of 3 to 8 times the same as the alcohol extraction method, but at 85 ℃ to 110 ℃, for 1 to 48 hours, preferably Preferably, after the first extraction for 2 hours to 12 hours, the remaining solid content is filtered, and then half the amount of water is added again to perform secondary extraction according to the same conditions as described above. After filtering the two extracts to remove solids, the filtrate can be concentrated by heating to prepare a final hydrothermal extract. Normally, 1.0 Kg of natural product is extracted, and after the decompression process, 400 g of concentrated extract is obtained. The final hydrothermal extract thus obtained is dissolved in a solvent such as water or ethyl alcohol to prepare a composition of the present invention.

The composition of the present invention can be used for the purpose of improving the alopecia disease aimed at by the present invention, furthermore, for the treatment and prevention, and the formulation is not particularly limited. In addition, it may be provided as a functional external preparation composition for the purpose of improving the symptoms of the skin diseases, it may be provided as a pharmaceutical composition for the purpose of treatment and prevention of the hair loss disease.

When the present invention is provided as a functional external preparation composition, it may be formulated into a transdermal dosage form, for example, lotions, ointments, gels, creams, patches or sprays, shampoos and the like.

In addition, when the present invention is provided in a pharmaceutical composition, the herbal extract may be used as it is or in the form of a pharmaceutically acceptable salt. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid, acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , Acid addition salts formed by benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid and the like can be used. In addition to acid addition salts, base addition salts such as sodium hydroxide, potassium hydroxide, triethylamine, tert-butylamine can also be used.

The pharmaceutical composition according to the present invention can be formulated by adding various auxiliaries used in medicine, such as carriers or other additives such as stabilizers, emollients, emulsifiers and the like, as long as they do not adversely affect the active ingredient.

In the pharmaceutical composition according to the present invention, the content of the herbal extracts described above may vary widely depending on the formulation and is in accordance with conventional methods.

Pharmaceutical compositions according to the invention may be administered parenterally, transdermal administration is preferred, in particular topical application is most preferred. It may be formulated in a conventional manner using solid or liquid excipients or diluents suitable for topical application. For example, it may be any suitable formulation such as ointment, cream, injection, powder, granule, tablet, spray.

Hereinafter, the present invention will be described in detail through examples and comparative examples using the above materials and methods, but the examples presented are not intended to limit the scope of the present invention.

Example 1 Preparation of Hair Growth Promoting Composition

According to the composition of Table 1 it was prepared by purchasing the raw material for medicine.

[Table 1] Composition of raw materials

Raw material name Composition ratio (wt%) One 2 Natural product composition 1 (one or two or more of whole leaf erosion, sky fly, kangjinhyang, snake radish Flying star 20 Sky / Gangjinhyang 10/10  Natural composition 2 Shouao 12 12 Black Beans 10 10 Wolfberry 10 10 licorice 8 8

Example 1-1 Preparation of Alcohol Extract

After washing and finely cutting 1 kg of the total weight of each natural medicinal herb, leaf or fruit, the lower alcohol, such as methanol and ethanol, preferably 70-95% ethanol was added four times by weight in a round bottom flask. A reflux condenser was attached at 75 ° C. for 4 hours and then heated to reflux extraction. The solids were removed using a filter paper or a conventional filtration device in the hot state without cooling the extract. The filtered filtrate was dried under reduced pressure using a rotary evaporator or a conventional concentrator to prepare 400 g of the final extract.

Example 1-2 Preparation of Hot Water Extract

Except for using 4 times the water, the same method as the alcohol extraction method, but the first extraction for 4 hours at 90 ℃, filtered and added to the remaining solids half the amount of the second extraction according to the same conditions as described above It was. The two extracts were filtered to remove solids, and then the filtrate was concentrated by heating. At this time, 1Kg of natural product was extracted and heated and concentrated to obtain 400 g of concentrated extract.

Example 2 Preparation of Hair Regrowth Composition Using Alcohol Extract

In the preparation examples of the composition below, the active ingredient is a lyophilisate and / or phospholipid of the complex extract obtained according to Example 1 above.

Example 2-1. Hair Regrowth Composition (Spray Agent)

Using the following components, the alcohol extract prepared in Example 1-1 and the auxiliary ingredient were added to the composition shown in Table 2 at the composition ratio described to prepare an alcohol extract-containing hair growth promoting composition to which the auxiliary ingredient was added, thereby preparing a spray agent. (Composition 1, Composition 2).

TABLE 2

Ingredient Classification ingredient Composition ratio (% by weight) Composition 1 Composition 2 Phospholipid OxPAPC 10 Natural Ingredients Alcohol / Heat Extract of Whole Leaf Eumseong / Gangjinhyang, Sewao, Black Bean, Wolfberry, Licorice 20 30 vitamin Vit B1, B2, B6, B12, Vit C Biotin, Calcium Pantothenate, Niacinamide, and Folic Acid 3 3 mineral Zinc, iron, calcium and magnesium One One Blood circulation improver Gamma Linoleic Acid, EPA and Tocopherols One One Scalp Penetrant Dimethylsulfoxide and Povidone 7 7 Skin irritant Menthol, peppermint One One Moisturizer glycerin 3 3 Solvent (Base) Purified Water and Ethyl Alcohol 54 54

Example 2-2 Hair Regrowth Composition (Shampoo)

Using the following ingredients, the alcohol extract prepared in Example 1-1 and the auxiliary ingredient were added to the composition shown in Table 3 at the composition ratio described to prepare an alcohol extract-containing hair growth promoting composition to which the auxiliary ingredient was added, thereby preparing a shampoo. .

TABLE 3

Ingredient Classification ingredient Composition ratio (% by weight) Composition 1 Composition 2 Phospholipid OxPAPC 10 Natural Ingredients Alcohol / Heat Extract of Whole Leaf Eumseong / Gangjinhyang, Sewao, Black Bean, Wolfberry, Licorice 20 30 Surfactants ALES 26 26 ALS 16 16 pH regulator NaOH 0.25 0.25 Thickener NaC1 One One Solvent (Base) Purified Water and Ethyl Alcohol 6 6

Example 3 Preparation of Hair Regrowth Composition Using Hot Water Extract

Example 3-1 Hair Regrowth Composition (Spray Agent)

The auxiliary component was added to the hydrothermal extract prepared in Example 1-2 in the same manner as in Example 2 to prepare a hydrothermal extract-containing hair growth promoting composition to which the auxiliary component was added, to obtain a spray agent (composition 3, composition 4). .

Example 3-2 Hair Regrowth Composition (Shampoo)

The auxiliary component was added to the hydrothermal extract prepared in Example 1-2 in the same manner as in Example 2 to prepare a hydrothermal extract-containing hair growth promoting composition to which the auxiliary component was added, to prepare a shampoo.

Comparative Example 1

Minoxyl 5% (Main ingredient: minoxidil 5%)

Ingredient Classification ingredient chief ingredient Minoxidil chief ingredient Salicylic acid chief ingredient Nicotinamide chief ingredient Tocopherol Supplementary Ingredients menthol

Experimental Example 1 Clinical Experiment of Hair Growth Promoting Effect

In the experiment used directly on the human body, hair growth composition (spray agent) and 5% formulation of minoxil according to the compositions of Examples 2 and 3 (composition 1 to 4) were tested on three patients with hair loss symptoms (Comparative Example 1). To apply 2cc twice every morning and evening. Three months after application, the number of hairs in a square of 1cm in width and 1cm in length was examined as a sample, and the average increase rate of the number of hairs was observed by comparing the number of hairs before use and the number of hairs after use. Shown in

TABLE 4

Average growth rate of hair Composition 1 Composition 2 Composition 3 Composition 4 Comparative Example 1 3 months 65% 66% 70% 72% 52%

In addition, 25% of the 12 patients were visually confirmed to have a hair growth effect after 1 month of applying the external composition for hair regrowth of the present invention, and 70% of hair loss patients visually confirmed the hair growth effect after 2 months. It became. In addition, after 3 months, the hair growth effect was visually confirmed in 20 hair loss patients.

Experimental Example 2 Clinical Experiment of Hair Loss Prevention Effect

In experiments used directly on the human body, the hair-repellent composition according to the hair regrowth composition (spray agent) of the composition 1 to composition 4 of Example 2 and 3, and the 5% formulation of minoxyl (Comparative Example 1) in each of three people with hair loss symptoms To apply 2cc twice every morning and evening. The average reduction rate of the hair loss is shown in Table 5 by comparing the number of hair loss before use and the number of hair loss after use after 3 months of application after the application of the number of hairs dropped immediately after the hair is closed.

TABLE 5

Average rate of hair loss Composition 1 Composition 2 Composition 3 Composition 4 Comparative Example 1 3 months 71% 68% 75% 73% 43%

In addition, it was confirmed visually that the hair loss prevention effect after 1 week to 2 weeks after the application of the composition for applying the external preparation for skin regrowth of the present invention.

Experimental Example 3 Skin Response Evaluation Experiment

Evaluation of skin reaction was judged using toxicology test criteria such as pharmaceuticals. In addition, the degree of irritation to the skin was assessed by Draize's P.I.I (Primary Irritation Index) calculation method.

In order to determine the degree of skin irritation with respect to the composition according to the present invention, the skin patch test was performed on four subjects with respect to the compositions (spray agents) according to Examples 2 and 3. Observation determined the skin reaction after 24 hours or 48 hours of patching. The skin reaction rate, which is a test result, is shown in Table 6 below.

Table 6 Skin Response Rate

Reaction Rate (%) 24 hours 48 hours Subject 1 Subject 2 Subject 1 Subject 2 Composition 1 (Example 2) 0.2 0.3 0.2 0.2 Composition 2 (Example 2) 0.2 0.2 0.2 0.2 Composition 3 (Example 3) 0.2 0.2 0.2 0.2 Composition 4 (Example 3) 0.2 0.2 0.1 0.2 Comparative Example 1 0.5 0.5 0.4 0.5

After applying the composition for hair regrowth (spray agent) of the present invention to a patient without hair on the head of the head, and comparing the number of hairs before use and the number of hairs after use, only two months have passed since the composition of the present invention was applied. In addition, hair loss prevention and hair growth promoting effect can be confirmed, and after 3 months, it can be confirmed that the hair loss is considerably disappeared.

As described above, the present invention is effective in preventing hair loss and promoting hair growth by combining phospholipids and herbal medicines that promote capillary production in relation to atrophy and disappearance of hair follicle cells. Will have an effect.

Claims (5)

(A) at least one member selected from the group consisting of anterior lobe vinegar, flyweed, gangjin-hyang, snake radish, and crab; And (B) extracting sewage, black beans, wolfberry and licorice using 40 alcohol at 40-90 ° C. or hot water extraction at 85-110 ° C .; Mixing the (A) and (B) extracts so that the weight ratio is 1: 1 to 3; Dissolving the mixture in water or ethyl alcohol to prepare a mixed solution; And A method for producing a composition for preventing hair loss and promoting hair growth, further comprising dissolving an auxiliary component in the mixed solution. The method of claim 1, further comprising dissolving phospholipid in the mixed solution. According to claim 1, wherein the auxiliary component is a blood circulation improving agent consisting of vitamins, minerals, gamma linolenic acid, EPA, tocopherol and mixtures thereof; Scalp penetrants consisting of dimethyl sulfoxide, triton-X and povidone; Glycerin, a skin moisturizer; And A method for producing a composition for preventing hair loss and promoting hair growth, characterized in that the skin stimulant consisting of menthol and peppermint. The method of claim 1, wherein the auxiliary component is a surfactant, a pH adjusting agent and a thickener, the method for producing a composition for preventing hair loss and promoting hair growth. A coating agent, ointment, cream, spray or shampoo comprising a composition for preventing hair loss and promoting hair growth prepared by the method of claim 1.