KR100761660B1 - A composition for promoting hair growth - Google Patents

Abstract

 The present invention relates to a composition containing a mixed extract consisting of sewage, cedar leaf, bone clam, baekbaekpi, earl pear, Angelica, goji berry, yellow, sperm, zelkova, bokbunja and jujube, the composition is safe for human body and prevents hair growth. Excellent promoting effect.

Sewage, Lateral Lobe, Hair Growth

Description

Hair growth promoting composition {A COMPOSITION FOR PROMOTING HAIR GROWTH}

Figure 1 is a photograph showing the results of the first hair growth efficacy test of Example 2-1, each photograph shows the degree of hair growth of the experimental group mice.

Figure 2 is a graph showing the results of the primary hair growth efficacy test of Example 2-1, showing the average hair length of the experimental group mice.

Figure 3 is a photograph showing the secondary hair growth efficacy test results of Example 2-2, each photograph shows the degree of hair growth of the experimental group mice.

Figure 4 is a graph showing the secondary hair growth efficacy test results of Example 2-2, showing the average hair length and thickness of the experimental group mice.

Figure 5 is a photograph showing the secondary hair growth efficacy test results of Example 2-2, each photograph shows the hair thickness and density of the experimental group of the mouse.

Hair is not life-related, but it does provide sex appeal. Hair protects the scalp from sunlight, and eyebrows and eyelashes protect the eyes from sunlight and sweat. The hair in the nose has the effect of filtering out external irritants, and the hair on the folds of the skin reduces friction. Hair is present everywhere on the skin except on the skin of the palms, soles of the feet, the distal skin of the fingers and toes, the borders of the mucous membranes and the glans, but in many parts of the body the hair exhibits different biological and morphological features. Hair, which begins in the fetus and grows into the elderly, is constantly changing in character, and under normal physiological conditions, the same hair follicle continues to produce different types of hair. The four types of hair covering the human skin are as follows.

      1) hair removal (waste, down, lanugo); At birth, a fetus develops in the mother's abdomen, about 20 weeks old, and is the first thin, light-colored hair that appears in life. 2) vellus hair; Delicate hair covering most of the body, and has a rich pigment depending on the part of the human body and is replaced by a thick, strong bell. 3) indeterminate hair, intermediate; It is a hair with an ambiguous distinction between soft hair and long hair. The distinction is based on its diameter rather than its length, most bristles are 20-40 microns in diameter, and most longitudinal hairs are 60-120 microns. 4) seed hair (female, terminal hair); Adult hair, eyebrows, eyelashes, beards, armpits, and pubic hair are all hairs. The extent to which hair hair is replaced by the hair is influenced by genetic predisposition and endocrine organs. For example, the hair on the scalp turns into a bald hair in both men, women, Koreans, and Westerners. The breast hair only turns into a bald hair in some Koreans or more Western male adults, and remains the soft hair in most women. The armpits and vulva also show a female hair in adult men and women of all races, and male plastic baldness, on the contrary, changes from a female hair to a fluffy hair, which varies considerably depending on the individual's endocrine function and genetic predisposition.

Looking at the structure of the hair, the hair follicle looks like a stem growing out of the plant bulb. This bulbous part is called hair follicles. At the bottom center of the hairball, there is a dermal papilla, where capillaries form a web. The area surrounding the breast nipple is called mosquito matrix (毛 基質). Mosquito matrix is composed of mosquito matrix cells and melanocytes and is very active in cell division, corresponding to the hair production plant. Differentiate from the mosquito matrix and migrate to the surface of the skin. The innermost part of this keratinized structure is hair (hair shafts). This hair is composed of three kinds of hair from the center to the outside of the hair, hair, and hair. In addition, this hair is surrounded by a sheath (hair root herb: inner hair root herb (+ 毛 根 墅) + outer hair root herb (外側 毛 根 墅)). There are melanocytes between the mosquito-cells in the hair follicles, and the melanin produced by the melanocytes is later devoured by the hair-forming cells to determine the color of the hair according to the amount of melanin.

      In addition, the human hair is about 100,000 to 150,000 pieces, each hair has a different cycle and grow and drop out through the anagen, catagen, telogen. This cycle is repeated over three to six years, with the result that on average 50 to 100 hairs fall out normally. Generally, the term alopecia refers to the increase in the number of hairs that fall abnormally due to shortening of the hair growth rate in the growth phase and more hair in the degenerative or resting phase. The causes of hair loss have been discussed such as poor blood circulation, excessive male hormone action, excessive sebum secretion, peroxide, bacterial deterioration of the scalp function, genetic factors, aging, stress. However, to date, no clear cause of hair loss is known, and in recent years, a growing number of people are suffering from hair loss due to changes in diet and stress caused by social conditions. It is increasing.

      Alopecia is classified into congenital alopecia and acquired alopecia. Congenital alopecia refers to the absence of hair from birth, and acquired alopecia refers to the loss of hair as it ages. In particular, acquired alopecia can not be found in non-cancerous alopecia and skin lesions, but can be classified into alopecia in pathological skin and scar alopecia.

It can also be classified as androgenetic alopecia, alopecia areata and other alopecia. About 50 to 100 hairs fall a day. Male hair loss is a symptom of a naturally reduced number of hairs that do not regenerate after being lost. This is often referred to as adult or mild alopecia. Most people who complain of alopecia are included in this type. This symptom is usually characterized after 25 years of age, but has recently been noticeably increasing in men around the age of 20. There are many cases of male alopecia, such as a rounded off from the parietal side, an M-shaped prominent head on both sides of the forehead, and a U-shaped forehead. The most common cause of male alopecia is genetic factors. However, not all genes are expressed, and the factor involved in gene expression is also male hormones. In other words, due to the effects of male hormones, men have more baldness than women, and do not become bald before puberty, and there is no baldness to those who castrated before puberty. In addition, there are the following theories as several causes. The cause of androgenetic alopecia is the hormonal theory that it comes from an imbalance between male and female hormones. In other words, the increase in the male hormone inhibiting the growth of the hair in the crown at the forehead, indicating this result. There is a scalp tension theory that the skull grows due to excessive use of the head, but the scalp cannot grow accordingly and the scalp is pulled and thinned, capillaries are compressed and the nutrition of the breast papilla is insufficient and the head is peeled off at the end. Persuasion is losing. There are different theories according to race, but the ratio of hair loss varies according to race. The reason for this is not yet clear, but statistically, Korea has a very low probability of hair loss compared to whites and blacks. But male alopecia is more common in developed countries than in less developed countries. There are reports of alopecia not found in real and primitive peoples, and very few in developing countries.

Alopecia areata is a phenomenon in which hair falls out in a circle. This alopecia areata may be confused with alopecia areata or feminine alopecia, but the cause and the size of the hair fall out are also completely different. Male alopecia is a physiological phenomenon, whereas alopecia areata is classified as pathological. The onset of hair loss usually starts at about 2 to 3 cm in diameter, and increases gradually in number and size as it progresses. Then the whole head may eventually be hair loss. In particular, alopecia areata is also called alopecia areata because it is difficult to treat hair loss phenomenon appearing on the back or side of the head. In alopecia areata, the prevalence rate is below 75% and is more than 75%, and it is a phenomenon that can occur in anyone, regardless of race or ethnic differences, as well as gender differences. Alopecia areata has a different name depending on the extent of hair loss. The widening range is called malignant alopecia areata, and malignant hair loss progresses further and all hair falls out. Frontal alopecia and all hair falling out are called general alopecia. There are many theories about the cause of alopecia areata, but there is no clear orthodoxy. At first, parasitic emergence was suggested, followed by fungal and then bacteria. Since the early 1900s, psychological and neurological causes of alopecia areata have been reported. Recently, it is known as an autoimmune disease.

In addition to the alopecia areata and alopecia areata, hair loss due to endocrine abnormalities, nutritional disorders, hair loss due to metabolic disorders, medications, and inflammation, infection tumors and other pollution, hair loss such as stride are very diverse.

Unfortunately, there is no complete hair loss treatment at present, and most hair loss and hair loss treatments on the market are designed to improve the blood circulation of the skin and to nourish the hair roots. Thus, the middle is still there is no silver bullet satisfactory regarding hair loss that is currently being registered as a pharmaceutical drug recognized to some extent, not only fascia and minoxidil program to obtain FDA approval.

Pharmacia & Upjohn has long developed minoxidil formulations and used them to treat hypertension with vascular relaxation. However, when taking this medicine, as a side effect, it grows up to 2-4cm of hair on the whole body of the head, arms, and legs.It is a hair repellent that is applied to make 2% minoxidil solution and released under the name of Rogaine. As the company's patent expires, other companies make similar products and start selling them cheaply, making 5% of their sales. Although the mechanism of minoxidil's hair growth is not known exactly, it is thought to promote hair growth by nourishing hair roots by increasing blood flow through vasodilating effect. This model of increased blood flow has recently been reported that minoxidil increases the expression of vascular endothelial growth factor (VEGF), a growth factor involved in vasodilation, in the dermal papilla, the main cell of hair follicles (Br. J. of Dermatol., 1998; 138; 407-411). In addition, in addition to the vasodilation effect in the hair growth effect mechanism of minoxidil, research reports that it promotes hair follicle cell activation of hair roots in vitro culture (Skin Pharmacol., 1996; 9; 3-8) and hair follicle growth in hair follicle tissue culture (J. Invest. Dermatol., 1989; 92; 315-320) suggest that minoxidil acts as a direct growth factor on the hair root. However, minoxidil preparations have scalp irritation symptoms such as dry scalp, pruritus and erythema as side effects, mainly due to propylene glycol or liquid base, and when using 2% solution, about 7% and 5% solution There is a lot more happening. Allergic contact dermatitis is also a very important side effect. However, the biggest drawback is that if the topical application is stopped, the hair growth effect is stopped within two months, and hair loss occurs again.

Finasteride (Propecia) is a treatment used by men to treat prostatic hypertrophy as they age. However, when the drug was prescribed and taken, it was reported that a new hair was found as a side effect. In the second half of 1997, the US Food and Drug Administration (FDA) recognized the efficacy and safety of the treatment of patients with male alopecia. It became. Propecia, developed by Merck, is an inhibitor of 5α-reductase, 1 mg of finasteride, which prevents testosterone from converting to 5α-dihydroxytestosterone (DHT). However, side effects such as impotence, decreased libido, and decreased ejaculation have been reported. To date, safety has not been established for women and children, and its use in pregnant women and women with possible pregnancy is strictly prohibited.

In addition, currently commercially available hair regrowth, Cheil Sugar's "weaving hair growth power" generates more than 10 billion won annually, its main ingredient is PDG (pentadecanoic acid glyceride) as a single substance. This is followed by “Spella 707” developed by Handok Cosmetics Hair Research Institute. It contains 12 kinds of herbal extracts such as almonds, Angelica, Ginseng and Cordyceps sinensis. In addition, the following hair growth and hair growth promoters are distributed in the market, but their efficacy is far below expectations. Nandamo (Indian soap ingredients), Dr. Mo (Pacific chemistry, Cornus extract), Morinaga (consisting of natural foods such as Daewon Pharmaceutical, black soybeans, and perilla), Grovis Beauty (Gyeongin Pharmaceuticals, cypress leaves and natural fatty acids), Solpyo Mosaengcheon 012 (Chosun fragrance, ginseng, pine needles, chitosan extract main ingredient) and NBH 958 (Costa World Co., Ltd., manufactured with fermented herbal extract).

In addition, many types of hair repellent-related patents have been published and registered in Korea. Representative recently disclosed patents are as follows.

Publication No. 2005-0119525 relates to compositions containing ellagic acid or derivatives thereof, and Publication No. 2004-0016331 discloses snow tongues (Nasturtium flowers), black horse, apple rock, gardenia, mint, mulberry and health. Extract is an active ingredient. Publication No. 2005-0064581 contains 12 kinds of Chinese medicinal herbs in ginseng leaf extract, which are rich, young yanghyang, Man Hyungja, Cheonggung, Baekji, Cheoncho, Sukjihwang, medicinal herb, Cornus, Mokdanpi, Taeksa, and Baekbokyeong. Publication No. 2005-0050321 relates to a composition in which natural copper fine powder is mixed with safflower oil, and Publication No. 2004-0062238 relates to a composition composed of placenta extract, ginseng extract, cheongung extract and garlic extract. Publication No. 2005-0040652 is based on Zahager Extract, Ginseng Extract and Cornus, and Publication No. 2004-0011864 is composed of Angelica, Chrysanthemum, Mint and Ginseng. Publication No. 2004-0001683 relates to extract extracts of pine needles, ginkgo biloba, mulberry root and the like, and Publication No. 2003-0080496 relates to preparations containing gossypol or derivatives thereof, such as cotton.

However, even when such chemical products and natural products are on the market, there is still no development of excellent formulations having certain efficacy. The domestic hair loss population is estimated at 3.5 million, or 23% of adults over 20 years of age, and even more, including women and pediatric hair loss populations, is an urgent need for good formulation.

The present invention provides a new hair growth promoting composition having excellent efficacy for preventing hair loss, promoting hair growth, and overall hair growth, without any side effects on the human body.

In one aspect, the present invention relates to a novel hair growth promoting composition. In particular, the present invention relates to a hair growth promoting composition containing a mixed extract consisting of sewage, baekhyeop, bone chain, baekbaekpi, Baekjak, Angelica, wolfberry, yellow, sage, seaweed, bokbunja and jujube.

Sewage, which is one of the constituents of the mixed extract, is known to have black hair when taken from ancient times, and has a tonic action and energy-enhancing effect, and anthraquinoids, chrysopamol, and emodin are known as main components. have. It also contains lecithin, which has the effect of improving blood circulation in the brain. The ephedra leaves the beard and eyebrows, treats hemorrhage, dysentery, etc., inhibits pathogenic trichomonas or gonorrhea, and has an effect of smoothing blood flow. Angelica protects the liver and kidneys in one shot, improves blood flow to the scalp, clears blood, and has the effect of healthy hair and hair roots. Sukjiwang blackens the gray hair, strengthens the bone marrow, bones and tendons, improves the circulation of the scalp, helps the growth of the scalp and hair, and prevents hair loss. Kelp is rich in carbohydrates and alginic acid in addition to pigments such as chlorophyll and carotene, and is rich in amino acids such as iodine, vitamin B _{2 and} glutamic acid. Alginic acid helps to release heavy metals and cholesterol in vitro, while iodine is also a major source of thyroid hormones for hair growth. Bokbunja is rich in organic acids, sugars, vitamin C, etc., nourish hair roots and improve blood circulation. The rest of the herbs further amplify the effect due to synergy. In other words, the hair growth promoting composition of the present invention contains components such as sewage, white leaf, white peony, bone clam, donkey, baekbaekpi, wolfberry, yellow, etc., which act on hair follicle epithelium and peripheral blood vessels to activate hair cells. There are phytoestrogens that treat endocrine incompatibility and contain isoflavone-rich epidermis.

The mixed extract of the present invention is an alcohol extract or hot water extract of mixed medicinal herbs, such as sewage, ephedra leaves. The alcohol extract is more preferable, and it is thought that alcohol extraction selectively extracts the active ingredient, while hot water extraction reduces the efficacy of unit gram sugar by extracting not only the active ingredient but also other non-active ingredients such as lignin.

As another embodiment, the present invention relates to a hair growth promoting composition further comprising an auxiliary component, in addition to twelve medicinal herbs such as sewage and ephedra. Auxiliary ingredients include vitamins, minerals, blood circulation improvers, scalp penetrants, skin irritants and / or moisturizers.

First, vitamins B ₁ , B ₂ , B ₆ , B ₁₂ , C, calcium pantothenate, nicotinic amide, folic acid, and biotin, and minerals such as zinc, iron, calcium, and magnesium may be added. Mixing and using these vitamins and minerals is preferable because it can enhance the bioactive effect. At this time, the vitamin and minerals are preferably contained in 0.1 to 10% by weight relative to the total weight of the final composition, in particular vitamins B1, B2 and C 0.01 to 3% by weight, 0.01 to 3% by weight relative to the total weight of the final composition And 0.01 to 4% by weight.

Blood circulation improving agent is added to improve the blood circulation and to enhance the biological activity effect, gamma linoleic acid, EPA, tocopherol and the like can be used. At this time, the blood circulation improving agent is based on the total weight of the final composition. It is preferably included in 0.1 to 0.5% by weight.

Scalp penetrants use dimethyl sulfoxide (DMSO), Triton-X or povidone, and are used to enhance the penetration of pharmaceutical components into the dermal layer deep into the scalp. In particular, the addition of menthol, a skin stimulant, increases skin irritation, which further enhances the effect of the pharmaceutical ingredient on the dermal layer of the skin. At this time, the scalp penetration agents such as dimethyl sulfoxide, triton-X, and povidone are each contained in an amount of 1 to 20% by weight based on the total weight of the final composition, and skin irritants such as menthol are preferably included in an amount of 1 to 5% by weight of the total weight of the final composition. In particular, when dimethyl sulfoxide, Triton-X and povidone, which are scalp penetrants, are used separately, 1.0 to 20% by weight of dimethyl sulfoxide, 1.0 to 10.0% by weight of Triton-X and 1.0 to povidone, based on the total weight of the final composition. It is preferably included in the 5.0 wt%. Especially, Older scalp layers, such as baldness, are difficult to penetrate into the usual active ingredients, so many kinds of hair growth and hair growth may be a major cause of their ineffectiveness. Among them, dimethyl sulfoxide (DMSO) is preferable as a scalp penetrant, and DMSO is a low molecular compound, which has a property of passing through a biological membrane well and increasing cell membrane permeability of other substances. DMSO also has an antioxidant action that removes hydroxy radicals (OH '). DMSO has the advantage of dissolving water, alcohol and almost all organic and biochemicals, and is widely used as a solvent for biopolymers in nuclear magnetic resonance analyzers. The mechanism by which DMSO helps infiltrate the scalp has not been elucidated, but it is believed to be due to the ability to instantly dissolve polymers such as proteins with excellent dissolving power.

Moisturizers are added to increase the moisturizing effect of the final composition to enhance the sustainability of the drug, with glycerin being preferred and added at 0.5 to 5% by weight relative to the total weight of the final composition.

In addition, the hair growth promoting composition of the present invention includes a solvent for dissolving extracts of sewage, ephedra, and the like, and various components such as various vitamins, minerals, and scalp penetrants, and such solvents may be water or ethyl alcohol. have.

On the other hand, the hair growth promoting composition of the present invention may be used directly extracts, it is preferable to be processed and prepared with a pharmaceutically acceptable carrier in accordance with pharmaceutical preparation methods known in the art. Examples of such carriers include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, Polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil. In addition, the hair growth promoting composition of the present invention may further comprise a filler, anti-coagulant, lubricant, wetting agent, fragrance, emulsifier or preservative.

Hair growth promoters according to the present invention may be formulated using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration. The formulations may be in the form of tablets, pills, granules, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders and the like. When prepared in the form of granules, liquids, etc. to enhance the taste, natural flavors such as plum, lemon, pineapple or herb, or natural pigments such as fruit juice, chlorphyllin and sweeteners such as fructose, honey, Sugar alcohols, sugars and the like can be mixed with an acidulant such as citric acid and sodium citrate.

In addition, the hair growth promoting composition according to the present invention can be administered through a variety of routes including scalp application, oral, etc., it is most preferred to use a method for administering the scalp application.

The daily scalp application amount of the final composition used as the hair growth promoter according to the present invention is in the range of 10 g to 300 g. However, the actual application amount should be determined in consideration of various related factors such as the application method, the age, sex, weight of the patient and the severity of the patient, and the application amount is not intended to limit the scope of the present invention in any aspect.

The composition of the present invention showed an excellent hair growth promoting effect as compared to the current commercially available control drugs, as confirmed in the examples below, and also used the formulation prepared in Example 1 to evaluate toxicity. As a result of measuring acute skin toxicity, 50% lethal dose (LD ₅₀ ) in ICR mice, the composition of the present invention was found to be very safe because it appeared to be more than 5g per kg body weight.

As another embodiment, the present invention relates to a method for preparing the hair growth promoting composition of the present invention.

The present invention is 1) Sewage, white leaf, bone chain, baekryepi, Baekjak, Angelica, Gugija, Hwangjeong, Sukjihwang, kelp, bokbunja and jujube medicinal herb extracted by heating under reflux for 1 to 48 hours at 40 ℃ to 90 ℃ After filtration, 2) drying the filtrate obtained in step 1) under reduced pressure to prepare a concentrated extract, and 3) dissolving the concentrated extract obtained in step 2) in a solvent, hair growth of the present invention. A method for preparing a facilitation composition.

More specifically, after washing each of the natural medicines cleanly, lower alcohols such as methanol and ethanol, preferably 70-95% ethanol, 3 to 8 times by weight ratio, 40 ℃ to 90 ℃, preferably Preferably at 60 ° C. to 85 ° C., heated to reflux extraction for 1 to 48 hours, preferably 2 to 12 hours, and the solids are removed using a filter paper or a conventional filtration apparatus in a hot state without cooling. The filtered filtrate is dried under reduced pressure using a rotary evaporator or a conventional concentrator to prepare the final alcohol extract. Normally, 2.0 Kg of natural products are extracted and 750 g of concentrated extract is obtained after decompression. The final alcohol extract thus obtained is dissolved in a solvent such as water or ethyl alcohol to prepare a composition of the present invention.

Alternatively, the present invention is 1) sewage, baekryeop, bone chain, baekbaekpi, Baekjak, Angelica, Goji berry, yellow jungjak, Sukjihwang, kelp, bokbunja and jujube medicinal herbs at 85 ℃ to 110 ℃ with water of 3 to 8 times Filtering by heating at reflux for 1 to 48 hours; 2) adding about half of the amount of water used in step 1) to the remaining solids after filtration in step 1) and filtering by heating under reflux for 1 to 48 hours at 85 ° C. to 110 ° C .; 3) preparing a concentrated extract by heating and concentrating the filtrate obtained in steps 1) and 2), and 4) promoting hair growth of the present invention, comprising dissolving the concentrated extract obtained in step 3) in a solvent. It relates to a method of preparing the composition.

More specifically, after washing each of the natural medicinal herbs, the same as the alcohol extraction method using pear to 8 times of water, but at 85 ℃ to 110 ℃, for 1 to 48 hours, preferably After 2 hours to 12 hours of primary extraction, the remaining solids were filtered, and then half the amount of water was added again, followed by secondary extraction under the same conditions as above. After filtering the two extracts to remove solids, the filtrate can be concentrated by heating to prepare a final hydrothermal extract. Normally, 2.0 Kg of natural product is extracted, and after the decompression process, 700 g of concentrated extract is obtained. The final hydrothermal extract thus obtained is dissolved in a solvent such as water or ethyl alcohol to prepare a composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to these embodiments.

Example  1: Preparation of Hair Growth Promoting Composition

      According to the composition ratio of Table 1 was prepared by purchasing the pharmaceutical raw material (purchased at the Kyungdong Market Pharmacy).

Composition ratio of raw material Natural medicine Composition ratio (wt%) Shouao 12.0 Temporal lobe 12.0 Bone Crest 10.0 White skin 10.0 Earl 10.0 Donkey  8.0 Wolfberry  8.0 Imperial  8.0 Hungjiwang  8.0 Kelp  6.0 Bokbunja  5.0 Jujube  3.0

1-1: Alcohol Extract Manufacturer

After washing and finely cutting the total weight of each natural medicinal herb, the total weight of the leaves or berries, finely divided into lower alcohols, such as methanol and ethanol, preferably 70-95% ethanol in a ratio of 4 times by weight to 75 in a round bottom flask. A reflux condenser was attached at 4 ° C. for 4 hours and then heated to reflux extraction. The solids were removed using a filter paper (No. 1001100 manufactured by Whatman) or a conventional filtration apparatus in a hot state without cooling the extract. The filtered filtrate was dried under reduced pressure using a rotary evaporator or a conventional concentrator to prepare 375 g of the final extract.

1-2: Hydrothermal  Preparation of Extract

Except for using 4 times the water, the same as the alcohol extraction method, but after the first extraction for 4 hours at 90 ℃, filtered and added to the remaining solids half the amount of the second extraction according to the same conditions as described above It was. The two extracts were filtered to remove solids, and then the filtrate was concentrated by heating. At this time, 1Kg of natural product was extracted and heated and concentrated to obtain 350g of concentrated extract.

1-3: Preparation of Alcohol Extract-Containing Hair Growth Promoting Composition Added Supplementary Components

As the alcohol extract prepared in Example 1-1 and the auxiliary component, the components shown in Table 2 were added at the composition ratios described to prepare an alcohol extract-containing hair growth promoting composition to which the auxiliary component was added.

In addition, an alcohol extract-containing hair growth promoting composition to which an auxiliary component (excluding the scalp penetrant) was added was prepared in the same manner as above except that the penetrant was excluded from the auxiliary components described in Table 2.

1-4: supplementary ingredients added Hydrothermal  Preparation of Extract-Containing Hair Growth Promoting Composition

An auxiliary component was added to the hydrothermal extract prepared in Example 1-2 in the same manner as in Example 1-3 to prepare a hydrothermal extract-containing hair growth promoting composition to which the auxiliary component was added.

The proportion of components of the final composition    Ingredient classification         ingredient Composition ratio (wt%)  Natural Ingredients Shou-O's Lateral Lobe and 10 other 18.0  vitamin VB ₁ , B ₂ , B ₆ , B ₁₂ , VC and Biotin 3.0  mineral Zinc, iron, calcium and magnesium 1.0 Blood circulation improvers Gamma Linoleic Acid, EPA and Tocopherols 1.0 Scalp Penetrant Dimethylsulfoxide and Povidone 8.0 Skin irritant menthol 1.0 Moisturizer glycerin 3.0 Solvent (Base) Purified Water and Ethyl Alcohol  65.0

Example  2: Animal efficacy test of hair growth promoting composition

2-1: Primary hair growth promoting efficacy test

2-1-1: Test group  Set

Animals were tested for the efficacy of the hair growth promoting composition of the present invention. 40 male 6-week-old C3H / HEN mice (Samtaco Korea) were acclimated for 7 days in a laboratory at 21 ± 3 ° C, 50 ± 5% relative humidity and 200-300 lux conditions, and only 30 healthy animals. Was used for testing. Distilled water was freely consumed as drinking water and experimental animal feed was freely consumed. After weighing animals that had no health problems during the adaptation period, 20 animals near the mean weight were selected. The selected animals were randomly divided into four groups of five animals. The first group was a negative control group applying only 10% ethanol solution, and the second group was a minoxidil 3% product (Hyundai Pharm. ), The third group is a group that does not include the scalp penetrant, wherein the scalp penetrant is excluded from the alcohol growth-containing hair growth promoting composition to which the auxiliary component prepared in Examples 1-3 of the present invention is added. The fourth group is formulated as a group to apply the hydrothermal extract-containing hair growth promoting composition to which the auxiliary component prepared in Example 1-3 (containing the scalp penetrant) Example 1-4 is added. It was.

Test group used for primary hair growth promoting efficacy test Applied medication First group 10% ethanol 2nd group Minoxidil 3% 3rd group Hair Growth Promoting Composition Containing Alcohol Extract Added Supplementary Ingredients (except Scalp Penetrant) 4th group Alcohol Extract-Containing Hair Growth Promoting Composition Fifth group Hot Water Extract-Containing Hair Growth Promoting Composition

2-1-2: Test Drug Application

Hair from the back of the C3H / HEN mouse to the tail of the mouse is first removed with an animal electric hair clipper (CHO-A, model name SM-127), and then a depilatory agent of thioglycolic acid (Taeguk Pharm., Viceroy). 10 minutes after the application of the cream), the hair was completely removed with a soft tissue. Since the depilatory agent is slightly toxic, the mice coated with the depilatory agent so as to prevent oral ingestion of the mice were put in a cage and then depilated. After 3 days of depilation, each test drug was applied.

In the first group, which was a negative control, 10% ethanol was uniformly applied to the dorsal side of the mouse depilated with a cotton swab. Similarly, the second group, the positive control group, was applied to the dorsal skin of 5 ml of product M each mouse, using a cotton swab. The third group was applied to the alcohol extract-containing hair growth promoting composition to which the auxiliary component (except scalp penetration agent) of the present invention was added 5ml per mouse using a cotton swab, and the fourth group was the alcohol extract-containing hair to which the auxiliary component was added The growth promoting composition was applied using a cotton swab at 5 ml per mouse. A hydrothermal extract-containing hair growth promoting composition to which the auxiliary agent of the fifth group was added was applied with a cotton swab 5 ml per mouse. The application was continued for 25 days in the same amount and in the same manner, and photographs were taken 14 days after the application of the time when the difference was most noticeable to the naked eye (FIG. 1). In the middle, 1cm above the tail), hair length was measured by magnifying glass to compare hair growth efficacy (Table 4, FIG. 2).

Average length of each group of mouse hair Experimental group Average length (cm) First group 5.66 2nd group 6.27 3rd group 8.60 4th group 10.08    Fifth group 8.95

As a result, the third group, the fourth group and the fifth group using the composition of the present invention showed an excellent hair growth promoting effect compared to the control group. In addition, the hair length of the penetrant-containing group was increased by 17% compared to the group without the penetrant, as can be clearly seen from the comparison between the third group (without penetrant) and the fourth group (with penetrant). This shows that when the hair growth promoting composition of the present invention contains a scalp penetrant, it can exhibit a more excellent effect. Compared to Group 4 and Group 5, the alcohol extract of natural products showed about 18% increase in hair length compared to the hydrothermal extract, because alcohol extraction effectively extracts the active ingredient. do.

2-2: Secondary hair growth promoting efficacy test

2-2-1: Test group  Set

30 male 6-week-old C3H / HE Slc mice (Samtaco Korea) were acclimated in a laboratory with a temperature of 21 ± 3 ° C., a relative humidity of 50 ± 5%, and a roughness of 200-300 lux for 7 days. Only those were selected and used for the test. Distilled water was freely consumed as drinking water and experimental animal feed was freely consumed. The animals were divided into 6 groups by randomly selecting the selected animals. The group 1 was negative control group applying only 10% ethanol solution, the group 2 was applied with weaving hair force (CJ, product J), and the third group was spela. 707 (Handok Cosmetics, product S below) is a positive control group, wherein the fourth group does not contain a scalp penetrant, and promotes hair growth containing alcohol extracts containing the auxiliary components prepared in Examples 1-3 of the present invention. In the composition to which the composition without the scalp penetrant is applied, the fifth group is an alcohol extract-containing hair growth promoting composition to which the auxiliary component (containing the scalp penetrant) prepared in Examples 1-3 is added, and the sixth group Was formulated as a group to which the hydrothermal extract-containing hair growth promoting composition to which the auxiliary component prepared in Example 1-4 was added was applied.

Test group used for secondary hair growth promoting efficacy test Applied medication First group 10% ethanol 2nd group Weaving hair 3rd group Spela 707 4th group Hair Growth Promoting Composition Containing Alcohol Extract Added Supplementary Ingredients (except Scalp Penetrant) Fifth group Alcohol Extract-Containing Hair Growth Promoting Composition 6th group Hot Water Extract-Containing Hair Growth Promoting Composition

2-2-2: Hair Growth Composition Application

In the same manner as in Example 2-1-2, hairs from the back of the neck to the tail of the C3H / HE Slc mouse were completely removed. After hair removal was stabilized for 3 days, 5 ml of each of the corresponding experimental medicines were uniformly applied to the entire hair of the mice and the like by using a cotton swab. The application was continued for 25 days, in the same amount and in the same manner, and photographs were taken 15 days after the application with the greatest visual difference (FIG. 3), on the last day the hair of the mouse was in the middle of each part (2 cm behind the neck, etc., Hair growth was measured by measuring the hair thickness and length with a magnifying glass (Table 6 and FIG. 4), and measuring the thickness of the hair and its density (gap between hair growth layers) using an electron microscope (SEM). Efficacy was compared (FIG. 5).

Average thickness and length of each experimental mouse hair Hair thickness (μm) Hair length (mm) First group 26.2 8.5 2nd group 36.2 9.5 3rd group 42.8 11.5 4th group 45.0 14.5 Fifth group 53.8 16.0 6th group 46.5 15.5

As a result, the fourth group, the fifth group, and the sixth group using the hair growth promoting composition of the present invention showed a great effect on hair length growth and thickness improvement, as compared with other controls, and as shown in FIG. 5, The hair interlayer spacing of the mouse to which the hair growth promoting composition of the present invention was applied was dense. This means that the density of the hair is high, which indicates that the hair of the same length has high hair loss resistance.

As described above, the composition of the present invention containing a mixed extract consisting of sewage, white leaf, bone chain, lettuce, white pear, Angelica, goji berry, yellow jung, sage, seaweed, bokbunja and jujube has no side effects on the human body and causes hair loss. It removes hormonal imbalance while radically eliminating and smoothing the scalp and shows excellent hair growth effects. In addition, when the scalp penetration agent is added to the composition of the present invention, it may exhibit an improved effect.

Claims (9)

A hair growth promoting composition comprising a mixed extract consisting of sewage, cedar leaf, bone clam, lettuce, bark pill, Angelica, goji berry, yellow goji, sage goji, kelp, bokbunja and jujube. The method of claim 1, wherein the mixed extract is 12% by weight of sewage, 12% by weight of ephedra, 10% by weight of bone clavicle, 10% by weight of lettuce, 10% by weight of Baekjak, 8% by weight of Angelica, 8% by weight of Gojiberry, 8% by weight of yellow, Hair growth promoting composition consisting of 8% by weight of Sukji sulfur, 6% by weight of kelp, 5% by weight of bokbunja and 3% by weight of jujube. The method of claim 1, further comprising: a vitamin; Mineral; Blood circulation improving agents consisting of gamma linoleic acid, EPA, tocopherol and mixtures thereof; Scalp penetrants consisting of dimethyl sulfoxide, Triton-X and povidone; Glycerin, a skin moisturizer; Menthol, a scalp stimulant; Or a hair growth promoting composition further comprising a combination thereof. The hair growth composition according to claim 3, further comprising a scalp penetration agent in the range of 1 to 20% by weight based on the total weight of the hair growth composition. The hair growth promoting composition according to claim 1, which is a coating agent. The hair growth promoting composition of claim 1, wherein the extract is an alcohol extract or a hydrothermal extract. 1) Sewage, white leaf, bone clam, baekbaekpi, Baekjak, Angelica, Gugija, Hwangjeong, Sukjihwang, Kelp, Bokbunja and jujube medicinal herb extracted under heating at reflux for 1 to 48 hours at 40 ℃ to 90 ℃ with methanol or filtered step, 2) drying the filtrate obtained in step 1) under reduced pressure to prepare a concentrated extract, and 3) A method for preparing the hair growth promoting composition of claim 1, comprising dissolving the concentrated extract obtained in step 2) in a solvent. 1) Sewage, baekhyeop, bone clam, Sangbaekpi, Baekjak, Angelica, Gugija, Hwangjeong, Sukjihwang, Kelp, Bokbunja and Jujube heated to reflux for 1 to 48 hours at 85 ℃ to 110 ℃ with 3 to 8 times the amount of medicinal herbs. Extracting and filtering; 2) adding about half of the amount of water used in step 1) to the remaining solids after filtration in step 1) and filtering by heating under reflux for 1 to 48 hours at 85 ° C. to 110 ° C .; 3) heating and concentrating the filtrate obtained in steps 1) and 2) to prepare a concentrated extract, and 4) A method for preparing the hair growth promoting composition of claim 1, comprising dissolving the concentrated extract obtained in step 3) in a solvent. The method of claim 7 or 8, wherein the solvent is water or ethyl alcohol.

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