KR102316334B1 - Food or Cosmetic Composition For Improving Hair Health Containing Complex Natural Product Extract - Google Patents

Abstract

The present invention relates to a composition for improving hair health containing, as an active ingredient, a complex herbal extract including 5 to 25% of a Siberian chrysanthemum extract, 65 to 85% of a peppermint extract, and 5 to 15% of a licorice extract, based on the weight of a dry composition.

Description

Food or Cosmetic Composition For Improving Hair Health Containing Complex Natural Product Extract for improving hair health containing natural product complex extract

The present invention relates to the use of a complex extract comprising gujeolcho, peppermint and licorice extract for improving hair health.

Hair loss refers to a state in which there is no hair in the area where hair should normally exist, and generally refers to the loss of hair on the scalp. Unlike soft hairs that have no color and are thin, if they fall out, it can cause cosmetic problems. Koreans, whose hair density is lower than that of Westerners, have about 100,000 hairs and can lose about 50 to 100 hairs per day.

To date, various causes have been suggested as the cause of hair loss. Genetic causes and androgens, which are male hormones, are considered to be important factors in the occurrence of androgenetic alopecia, and it is estimated that some of female pattern hair loss occurs in the same path as male pattern hair loss, but there is a clinical difference in the pattern. Alopecia areata is thought to be an autoimmune disease. Alopecia at telogen is temporary hair loss that occurs after severe physical and mental stress such as endocrine disease, nutritional deficiency, drug use, childbirth, fever, and surgery.

Gujeolcho is a dicotyledonous perennial plant of the order Asteraceae of the order Asteraceae. It grows in grassy fields at the foot of the mountain. It is about 50cm high, and the underground stem extends long sideways and reproduces. The shape is similar to that of Sangujeolcho, and the leaves on the root and the leaves at the bottom are divided into pinnate once. The leaves are egg-shaped, with a flat or heart-shaped lower part, and the upper edge is split like a wing. Gujeolcho, a herbal medicine, is the dried stem and leaves, and in oriental medicine and folk medicine, the entire flowered grass is prescribed for toothache, gynecological disease, and gastrointestinal diseases. Similar species, such as Sangujeolcho and Bawigujeolcho, are also used medicinally. It is distributed in Korea, Japan, China and Siberia.

Peppermint is a dicotyledonous perennial plant in the order Lamiaceae, and essential oil is contained in the leaves. If you prune frequently, it will grow into large stems, and if you cut the stem with 8~10cm left during the summer flowering period, it can be harvested in the fall. Raw leaves or stems dried in a well-ventilated shade are also used as cooking ingredients.

Licorice is called Ural licorice, bent licorice, and grows in Siberia, Iran, Afghanistan, Pakistan, China and Mongolia, and is also cultivated in Korea. European licorice is distributed in Southern Europe, Central Asia, and China. Variants of European licorice include Russian licorice, Persian licorice, and Iranian licorice, but they are not medicinal. In Korea and Japan, parts of the roots and stems of licorice (Glycyrrhiza uralensis Fischer), European licorice ( Glycyrrhiza glabra L.), or other flora and fauna are used with or peeled off. In China, the dried root of Glycyrrhiza uralensis Fischer (甘草), Glycyrrhiza glabra L. and Glycyrrhiza inflata Batal is used.

On the other hand, in the case of a complex extract derived from a natural product known for preventing hair loss or promoting hair growth, Korean Patent Application Laid-Open No. 2016-0136982 discloses a composition for preventing hair loss and promoting hair growth of a complex extract including Gujeolcho, and a method for producing the same, Korean Patent Application Laid-Open No. 10-2009-0076671 discloses a method of preparing a composition for preventing hair loss and promoting hair growth of a complex extract including peppermint, and Korean Patent Publication No. 10-2018-0096919 discloses a complex extract containing licorice for preventing hair loss and A composition for promoting hair growth is disclosed.

However, there is no disclosed a composition for preventing hair loss and promoting hair growth comprising the gujeolcho, peppermint and licorice as an active ingredient in a specific mixing ratio of the present invention.

Korean Patent Publication No. 2016-0136982 Korean Patent Publication No. 10-2009-0076671 Korean Patent Publication No. 10-2018-0096919

As a result of diligent efforts to develop natural products useful for hair health, the present inventors have found that the complex extract prepared by mixing gujeolcho, peppermint and licorice in a specific mixing ratio has excellent effects on preventing hair loss or improving hair growth, and that it is also excellent in anti-inflammatory and antioxidant effects. It was confirmed that the present invention was completed.

Accordingly, it is an object of the present invention to provide a hair health improvement use of gujeolcho, peppermint and licorice complex extract.

In addition, it is an object of the present invention to provide an anti-inflammatory or antioxidant use of the complex extract.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and the problem to be solved by the present invention can be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention provides a complex herbal extract comprising a gujeolcho extract, a peppermint extract and a licorice extract. The complex extract includes gujeolcho extract, peppermint extract and licorice extract as active ingredients, and 5-25% of gujeolcho extract, 65-85% peppermint extract and 5-15% of licorice extract based on the weight of the active ingredient. characterized. The complex extract may be usefully utilized for hair health. Preferably, the complex extract may include 10 to 20% Gujeolcho extract, 70 to 80% peppermint extract, and 5 to 15% licorice extract.

In the present invention, the extract refers to a material obtained by separating from gujeolcho, peppermint or licorice, and specifically may be an extract of a specific solvent, a crude extract, or a concentrate thereof.

The extraction solvent used to obtain the gujeolcho, peppermint or licorice extract of the present invention may be used without limitation, but preferably water, C ₁ to C ₄ lower alcohol or a mixture thereof. For example, the solvent used to obtain the gujeolcho and licorice extract among the complex extracts of the present invention is water, and the solvent of the peppermint extract may be alcohol, but is not limited thereto.

In the present invention, the term “alcohol” refers to an aqueous ethanol solution, for example, an edible ethanol solution used in food. According to the purpose of manufacturing the complex extract of the present invention, the content of ethanol contained in the alcohol of the present invention may be, for example, 10 to 90%, 35 to 65%, but is not limited thereto.

The extract of the present invention may include, without limitation, not only the extract by the water extraction solvent, but also the extract that has undergone a conventional purification process thereafter. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity, or affinity), etc. The fraction obtained through the process is also included in the extract of the present invention. In addition, the extract of the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

The complex extract comprising the gujeolcho extract, peppermint extract and licorice extract of the present invention as an active ingredient may be prepared through the following process, but is not limited thereto.

1) drying and grinding Gujeolcho, peppermint and licorice, respectively;

2) obtaining an extract by extracting Gujeolcho, peppermint and licorice, respectively;

3) filtering each extract and then concentrating under reduced pressure; and

4) drying each extract;

5) Mixing each extract with 5 to 25% of Gujeolcho extract, 65 to 85% of peppermint extract, and 5 to 15% of licorice extract based on dry weight.

Gujeolcho, peppermint and licorice used in step 1) can be used without limitation, those that are naturally grown, those that are grown directly, or those that are commercially available.

For the extraction in step 2), immersion (cold-chim, warm-chim) extraction, hot water extraction, ultrasonic extraction, supercritical extraction, or reflux extraction may be used without limitation. For example, the extraction temperature of the extraction may be 40 to 100° C., the extraction time may be 1 hour to 10 hours, and the number of extractions may be 1 to 5, but is not limited thereto.

Here, gujeolcho, peppermint and licorice can be obtained extracts under the same or different extraction conditions, respectively. Specifically, the gujeolcho extract can be obtained by extraction with water 5 to 50 times the weight of the dried gujeolcho extract at 60 to 100° C. for 2 to 12 hours. The peppermint extract can be obtained by extracting 5 to 50 times the weight of the dried peppermint extract with alcohol (eg, 50% aqueous ethanol solution) at 40 to 80° C. for 2 to 12 hours. Licorice extract can be obtained by extraction with 2 to 50 times the weight of dry licorice at 40 to 80° C. for 2 to 12 hours.

In step 3), each extract may be concentrated under reduced pressure at a temperature of 60° C. or less.

In one aspect, the present invention provides a composition for preventing hair loss or improving hair growth, comprising as an active ingredient a complex herbal extract comprising 5 to 25% of gujeolcho extract, 65 to 85% of peppermint extract, and 5 to 15% of licorice extract. The composition may be a food or cosmetic composition.

The complex extract of the present invention is characterized in that it has a hair cell proliferation promoting effect. As a result of the treatment of the complex extract of the present invention on the hair cells, the present inventors showed that the hair cell proliferation promoting effect was improved in comparison with the single plant extract constituting the complex extract and as compared to the complex extract with different compounding ratios for each plant extract. Excellent was found for the first time (FIG. 1).

Hair generation in hair follicles has repeated cycles of anagen, catagen, and telogen, and alopecia occurs by shortening the anagen phase or lengthening the catagen and telogen phases. Testosterone, a male hormone, works with 5α-reductase (5-AR) in dermal papilla cells to produce dihydrotestosterone (DHT). The generated DHT binds to androgen receptors in dermal papilla cells, thereby shortening the growth phase of hair follicles, causing androgenetic alopecia. Currently, finasteride and minoxidil are the hair loss treatments approved by the U.S. Food and Drug Administration. The finasteride inhibits the activity of 5α-reductase to delay the progression of hair loss and induce hair growth. Minoxidil is known to induce a hair growth effect by increasing nutrient supply through vasodilation and opening potassium channels. However, minoxidil cannot treat the root cause of alopecia, so the product must be used permanently, and hair loss appears again when use is stopped.

The composition according to the present invention is characterized in that it inhibits the expression of 5α-reductase. The present inventors treated the complex extract of the present invention on testosterone-treated human follicle dermal papilla cells (HFDPC). It was discovered for the first time that the effect of inhibiting the expression of 5α-reductase was excellent compared to the complex extracts having different compounding ratios for each extract (FIG. 5). In addition, it was confirmed that the complex extract according to the present invention can induce androgen receptor inhibitory effects and desirable morphological changes in cells in testosterone-treated HFDPC. Therefore, the complex extract according to the present invention can be usefully used as a composition for preventing hair loss or improving hair growth.

In one aspect, the present invention provides an anti-inflammatory or antioxidant composition containing as an active ingredient a complex herbal extract comprising 5 to 25% of gujeolcho extract, 65 to 85% of peppermint extract, and 5 to 15% of licorice extract.

The composition is characterized in that it has an inhibitory effect on the production of reactive oxygen species in immune cells. As a result of investigating the effect of the extract on the increased ROS level on immune cells stimulated by LPS, the present inventors compared the single plant extract constituting the complex extract in the complex extract of the present invention, and the compounding ratio for each plant extract It was found for the first time that the effect of reducing the level of ROS was excellent in comparison with the complex extracts different from each other (FIG. 2). Therefore, the composition of the present invention can be usefully utilized as an antioxidant composition.

The composition is characterized in that it has a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability. As a result of measuring the DPPH radical scavenging ability of the extract according to the present invention, the present inventors first discovered that the DPPH radical scavenging effect was excellent in the complex extract of the present invention compared to the complex extract having different compounding ratios for each plant extract (FIG. 3) . In addition, it was confirmed that the complex extract of the present invention has excellent ABTS radical scavenging ability in addition to DPPH radical scavenging ability. Therefore, the composition of the present invention can be usefully utilized as an antioxidant composition.

The composition is characterized in that it has an inhibitory effect on nitric oxide production in immune cells. As a result of examining the effect of the extract on the production amount of NO in immune cells, the present inventors compared the single plant extract constituting the complex extract in the complex extract of the present invention, and compared with the complex extract with different compounding ratios for each plant extract It was first discovered that the effect of inhibiting NO production was excellent ( FIG. 4 ). In addition, the ability to inhibit the production and expression of PEG2, iNOS, COX-2, IL-6 and IL-1β in the complex extract according to the present invention was confirmed. Therefore, the composition of the present invention can be usefully used as an anti-inflammatory or antioxidant composition.

The composition may be prepared in the form of a pharmaceutical composition, health functional food, general food, quasi-drugs or cosmetics. Specifically, the complex extract of the present invention can be utilized as a cosmetic or health functional food composition.

To the composition of the present invention, one or more selected from a thickener, an ionic surfactant, a solubilizer, a preservative, an essence and a functional additive may be added within a conventional range based on the complex extract.

When the complex extract of the present invention is used as an external composition for skin external application for preventing hair loss or improving hair growth, additionally fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or external preparations for skin It may contain adjuvants commonly used in the related field, such as any other ingredients commonly used for In addition, the components may be included in an amount conventionally used in the related art. The external preparation for skin is preferably in any one form selected from the group consisting of creams, lotions, ointments, gels, mucus, sprays and poultices, but is not limited thereto.

When the composition of the present invention is prepared in cosmetic form, hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow hair growth agent , can be manufactured in various forms such as eyelash hair growth agent and eyelash nutrition agent, and can be used for manufacturing or processing devices for scalp or hair, such as wigs or hats.

In the present invention, health functional food refers to a food having a biological control function such as prevention or improvement of disease, biological defense, immunity, recovery from illness, and suppression of aging, and should be harmless to the human body when taken for a long time. The complex extract of the present invention may be included in health functional food for the purpose of preventing hair loss or improving hair growth. The mixed amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment).

In the present invention, the health beverage composition may include various flavoring agents or natural carbohydrates as additional ingredients, like a conventional beverage. As the above-mentioned natural carbohydrates, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharin and aspartame may be used.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, It may include a carbonation agent used in carbonated beverages, and the like. In addition, the composition of the present invention may contain the pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination.

The complex extract of the present invention promotes the proliferation of skin cells necessary for hair growth, and has an excellent inhibitory effect on free radicals such as radical scavenging activity for antioxidant activity, as well as an inhibitory effect on inflammatory factors such as nitric oxide and follicular dermal papilla (breast papillae). ) can be widely used as a safe and useful hair improvement composition by contributing to the desired expression pattern of hair loss-related genes in cells.

1 shows the results of measuring the effect of the complex extract according to the present invention on the proliferation of hair cells,
Figure 2 shows the results of measuring the intracellular active oxygen removal effect of the complex extract according to the present invention,
3 shows the results of measuring the DPPH antioxidant effect of the complex extract according to the present invention,
Figure 4 shows the results of measuring the intracellular nitric oxide production inhibitory effect of the complex extract according to the present invention,
5a to 5c show the results of measuring the 5α-reductase expression inhibitory effect in skin cells of the complex extract according to the present invention.

Hereinafter, preferred preparation examples and examples are presented to help the understanding of the present invention. However, the scope of the present invention is not limited from the following Preparation Examples and Examples, and all ranges that can be easily understood and reproduced by those skilled in the art using the technical common sense in the field of natural products with reference to the following Preparation Examples and Examples is included in the present invention.

<Example 1> Preparation of complex extracts and experimental materials

1. Manufacturing method of complex extract

In this example, a complex extract was prepared as follows.

4 L of purified water was added to 200 g of dried gujeolcho and extracted at 85° C. for 6 hours to obtain an extract of gujeolcho. Thereafter, the filtered extract was concentrated under reduced pressure at 50° C. or less and then freeze-dried to prepare a Guulcho extract.

4 L of 50% alcohol was added to 200 g of dried peppermint and extracted at 50° C. for 6 hours to obtain a peppermint extract. Thereafter, the filtered extract was concentrated under reduced pressure at 50° C. or less and then freeze-dried to prepare a peppermint extract.

2 L of purified water was added to 200 g of dried licorice, and extracted at 50° C. for 6 hours to obtain a licorice extract. Thereafter, the filtered extract was concentrated under reduced pressure at 50° C. or less and then freeze-dried to prepare a licorice extract.

Based on the weight of the dry extract, the weight ratio of each gujeolcho, peppermint, and licorice extract was prepared as shown in Table 1 below to prepare complex extracts 1 to 6, respectively.

[Table 1]

2. Experimental Materials

Human Follicle Dermal Papilla Cells (HFDPC) and Follicle Dermal Papilla Cell Growth Medium were purchased from Promocell (Heidelberg, Germany). The HaCaT cell line was purchased from CLS Cell Lines Service (Eppelheim, Germany), and the Raw264.7 cell line was purchased from ATCC (American Type Culture Collection, Rockville, MD, USA). Griess reagent, LPS (lipopolysaccharides) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and EZ-Cytox was purchased from Dogenbio (Seoul, Korea). Carboxy-H2DCFDA and SRD5A1 antibody were purchased from Invitrogen (Waltham, MA, USA), and β-actin was purchased from BD Biosciences (CA, USA). Human SRD5a2 ELISA Kit was purchased from elabscience (Houston, TX, USA). DMEM (Dulbecoos modified Eagle's medium), FBS (fetal bovine serum) and RIPA (Radioimmunoprecipitation assay) buffer were purchased from Invitrogen (Waltham, MA, USA).

<Example 2> Measurement of hair cell proliferation promoting effect

Cell viability was measured to confirm the keratinocyte proliferation promoting effect of the complex extract according to the present invention. Hair follicles, as cells that produce hair while in the dermal papilla tissue, absorb nutrients from capillaries flowing in the dermal papilla under the hair follicle, divide and proliferate to form hair. Therefore, by measuring the effect on the proliferation of hair cells, it is possible to evaluate the effect of the complex extract according to the present invention on hair growth promotion.

Specifically, HaCaT cells were aliquoted in a 96 well plate at a concentration of 5×10^4 cells/ml and cultured for 24 hours, and then, the extracts of Preparation Example and Minoxidil were added at a concentration of 100 μg/ml or 0.25 μM. Incubated for 20 hours after injection. After that, 10 μl of EZ-Cytox was injected into each well and absorbance was measured at 450 nm 4 hours later. Cell viability was calculated as a percentage compared to the sample-free group.

As a result, in the experimental group treated with the complex extract No. 5 according to the present invention, 27% of the hair cell growth promoting effect compared to the negative control group was shown, and the hair cell proliferation promoting effect compared to the single extract of Gujeolcho, peppermint, licorice extract or other compounding ratios was confirmed to be excellent (Fig. 1).

<Example 3> Measurement of intracellular ROS removal effect

In order to confirm the oxidative stress inhibitory effect of the complex extract according to the present invention, the ROS removal effect was confirmed through flow cytometry. Specifically, raw264.7 cells were aliquoted in a 6 well plate at a concentration of 2×10^5 cells/ml and cultured for 24 hours, and then the extracts and Minoxidil were injected at a concentration of 100 μg/ml or 0.25 μM 1 hour after injection. After stimulation with LPS at 1 μg/ml, the cells were incubated for 24 hours. Thereafter, the cells were harvested, washed twice, and suspended in PBS, and Carboxy-H2DCFDA was injected so as to become 1 μM. After 30 minutes, the FITC emission spectrum was measured using a flow cytometer. The results are shown in FIG. 2, where the number of cells scattered with green fluorescence by the ROS marker (Carboxy-H2DCFDA) is shown on the X-axis.

As a result, it was confirmed that the intracellular ROS level was increased by LPS. However, surprisingly, it was confirmed that the ROS level was significantly reduced compared to the complex extract of gujeolcho, peppermint, licorice single extract or other compounding ratios when treated with the complex extract No. 5 according to the present invention (Fig. 2).

<Example 4> Measurement of DPPH antioxidant

In order to confirm the antioxidant effect of the complex extract according to the present invention, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability was measured. For the measurement of DPPH antioxidant, Blois's method (1958) was modified and used. In order to measure the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability, the natural extract of the present invention was sequentially diluted to 50 μg/ml and used. 150 μl of 0.2 mM DPPH dissolved in 95% ethanol and 50 μl of each natural product extract sample were placed in a 96-well plate, mixed by pipetting, and reacted in the dark for 30 minutes. The concentration of the remaining radicals was measured at 517 nm using a UV spectrophotometer. The scavenging ability of the extract for DPPH was expressed as a percentage by calculating the scavenged radicals through the concentration of the remaining radicals.

As a result, the experimental group treated with the complex extract No. 5 according to the present invention exhibited a DPPH scavenging ability of 60% or more, confirming that the DPPH scavenging ability was superior to that of the complex extracts of other compounding ratios (FIG. 3).

<Example 5> Measurement of NO (nitric oxide) production inhibitory effect

The inhibitory effect of NO (nitric oxide) production was measured for the complex extract according to the present invention. Specifically, raw264.7 cells were aliquoted in a 24 well plate at a concentration of 2×10^5 cells/ml and cultured for 24 hours, and then the extracts and Minoxidil were injected at a concentration of 100 μg/ml or 0.25 μM 1 hour after injection. After stimulation with 1 μg/ml LPS, the culture medium was collected after incubation for 24 hours. 100 μL of the collected culture solution and 100 μL of Griess reagent were injected into a 96 well plate and reacted for 15 minutes. After the reaction was completed, the absorbance was measured at 540 nm. The amount of NO production was calculated by substituting the standard curve prepared using _{NaNO 2 .}

As a result, it was confirmed that when the NO production increased by LPS was treated with the No. 5 complex extract according to the present invention, a significant reduction effect was exhibited compared to the gujeolcho, peppermint, licorice single extract or the complex extract of other compounding ratios (FIG. 4).

<Example 6> Measurement of the inhibitory effect of 5α-reductase 2 expression in testosterone-treated dermal papilla cells

Steroid 5α-reductase 2 inhibitory effect was measured by Western blot analysis and ELISA analysis for the complex extract according to the present invention. Specifically, HFDPC (human follicle dermal papilla cell) cells were aliquoted at a concentration of 4 × 10^5 cells/ml and cultured for 24 hours. Alternatively, 1 hour after injection at a concentration of 0.25 μM, stimulation with a concentration of 100 μM of Testosterone and incubation for 24 hours. Thereafter, the culture medium was collected and measured for ELISA kit analysis. For Western blot analysis, the cells were centrifuged twice with PBS, RIPA buffer was injected, and the protein was extracted by centrifugation at 12,000 rpm at 4°C for 15 minutes. Each of the extracted proteins was quantified by measuring absorbance at 595 nm using Bradford protein assay reagent. After the quantified protein was separated through SDS-PAGE, it was transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad) at 100 V for 1 hour. The membrane was blocked in TBS-T (Tris buffered saline with 1% Tween 20) containing 5% BSA (bovine serum albumin) at room temperature, washed 3 times with TBS-T for 10 minutes, and then anti-5α-reduced with primary antibody Enzyme (5-AR) and anti-β-actin were used to react at 4° C. for 16 hours. Then, after washing 3 times for 10 minutes with TBS-T, the secondary antibody (goat anti-rabbit IgG HRP, rabbit anti-mouse IgG HRP) was reacted in 5% BSA at room temperature for 2 hours and washed 3 times for 10 minutes with TBS-T did. Thereafter, the washed membrane was imaged using a detection system (FIG. 5A).

As a result, the single extracts of Gujeolcho, peppermint and licorice did not show a significant inhibitory effect on Steroid 5 5α-reductase 2 (5-AR2) expression, but the complex extracts showed an inhibitory effect. The effect was the most excellent in the compound extract No. 5 according to the present invention, and surprisingly, it was superior to minoxidil as a control material (FIG. 5B). As a result of the ELISA kit analysis, the compound extract No. 5 showed the best inhibitory effect (FIG. 5C).

Claims (10)

Containing as an active ingredient a complex herbal extract comprising 5 to 25% by weight of Gujeolcho extract, 65 to 85% by weight of peppermint extract, and 5 to 15% by weight of licorice extract,
Here, the herbal extract is _{extracted with water, C 1} ~ C ₄ lower alcohol or a mixture thereof at a temperature of 40 to 100 ° C. for 1 hour or more,
A composition for preventing hair loss or improving hair growth, which does not contain one or more of Angelica asiatica extract, ginkgo leaf extract and centella asiatica extract. The method of claim 1, wherein Gujeolcho is extracted with 5 to 50 times the weight of dried Gujeolcho with water at 60 to 100°C for 2 to 12 hours, and the peppermint extract is extracted with 5 to 50 times the weight of dry peppermint with alcohol at 40 to 80°C. The composition is extracted for 2 to 12 hours, and the licorice extract is extracted with 2 to 50 times the weight of dry licorice at 40 to 80° C. for 2 to 12 hours. The composition according to claim 1, characterized in that it has an effect of promoting hair cell proliferation. The composition of claim 1, wherein the composition inhibits the expression of 5α-reductase (5-AR). The composition according to claim 1, characterized in that it exhibits anti-inflammatory and antioxidant effects. Containing as an active ingredient a complex herbal extract comprising 5 to 25% by weight of Gujeolcho extract, 65 to 85% by weight of peppermint extract, and 5 to 15% by weight of licorice extract,
Here, the herbal extract is _{extracted with water, C 1} ~ C ₄ lower alcohol or a mixture thereof at a temperature of 40 to 100 ° C. for 1 hour or more,
An anti-inflammatory composition that does not contain one or more of Angelica asiatica extract, Ginkgo biloba extract and Centella asiatica extract. The composition according to claim 6, characterized in that it has an inhibitory effect on the production of active oxygen and nitric oxide in immune cells. Containing as an active ingredient a complex herbal extract comprising 5 to 25% by weight of Gujeolcho extract, 65 to 85% by weight of peppermint extract, and 5 to 15% by weight of licorice extract,
Here, the herbal extract is _{extracted with water, C 1} ~ C ₄ lower alcohol or a mixture thereof at a temperature of 40 to 100 ° C. for 1 hour or more,
An antioxidant composition that does not contain one or more of Angelica asiatica extract, Ginkgo biloba extract and Centella asiatica extract. The composition according to claim 8, characterized in that it has the ability to inhibit active oxygen production and scavenging radicals. 10. The composition according to any one of claims 1 to 9, which is a cosmetic or food composition.

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