KR102006665B1 - Anti-oxidant, anti-inflammatory, whitening and moisturizing cosmetic composition comprising the mixed herbal extract of silver weed, heather, and milk thistle as an active ingredient and preparation method of the same - Google Patents

Abstract

The present invention relates to an antioxidant, anti-inflammatory, whitening and moisturizing cosmetic composition comprising a mixed herbal extract of silverweed, heather, and milk thistle as an active ingredient, and a preparation method thereof.

Description

Anti-oxidant, anti-inflammatory, whitening, and moisturizing functional cosmetic composition containing herbal mixed extract of silverweed, hide, milk thistle as an active ingredient, and a method for preparing the same {Anti-oxidant, anti-inflammatory, whitening and moisturizing cosmetic composition comprising the mixed herbal extract of silver weed, heather, and milk thistle as an active ingredient and preparation method of the same}

The present application relates to an antioxidant, anti-inflammatory, whitening, and moisturizing functional cosmetic composition containing the herb extract of silverweed, hide, milk thistle as an active ingredient, and a method for producing the same.

Silver weed, Potentilla Anserina, is a perennial flowering plant belonging to the Rosaceae family and has been used as a medicinal herb for treating malnutrition, anemia, diarrhea and bleeding for thousands of years. The main component of the genus Potentilla is the ellagic acid type tannin, which contains a monomer and dimer ellagitannin similar to those found in green tea. Due to its high tannin content, it is known to be useful for sore throats, mouth and skin ulcers, bleeding and diarrhea. It also contains antioxidant flavonoids (quercetin, myricetin glycosides) and proanthocyanidin. Also, the leaves of silver Weed against H. pylori, flower has been found to showing an antibacterial effect against H. pylori, M. luteus, S. aureus , B. subtilis.

Heather, Calluna vulgaris , belongs to the dicotyledonous azalea family, native to northern Europe and Asia, and has traditionally been used as an antiseptic, antibacterial, diuretic, and antirheumatic agent. Heaters contain quercitrin and myrcitrin, one of tannins, arbutin, saponins, carotene and flavonoids. It is also known to have a detoxifying effect traditionally associated with arthritis, gout and rheumatism pain.

Milk thistle ( Silybum marianum ) is called white thistle. When you open the leaves of milk thistle, you can see the pattern of spilling white milk, and when you break the stem, the milky white sap comes out. All leaves, roots, stems, and seeds can be used, but the seeds and fruits are particularly helpful for liver health. Currently, it is known that seeds and berries of milk thistle are used as food ingredients, and milk thistle extract is used as a general medicine.

Prior art related to the present application, there is Republic of Korea Patent No. 10-1607445, but this relates to the novel milk thistle fruit extract and liver disease treatment and prevention, Republic of Korea Patent No. 10-1611349 relates to the formulation of Milk Thistle X As such, there is no study on the antioxidant, anti-inflammatory, whitening and moisturizing functional cosmetic composition containing the herbal blend extract of Silverweed, Heather, Milk Thistle as an active ingredient, and a method for preparing the same. Under this background, the present inventors have completed the present application by earnest research efforts to develop an antioxidant, anti-inflammatory, whitening and moisturizing functional cosmetic composition containing the herbal blend extract of silverweed, hide, milk thistle as an active ingredient.

An object of the present application is to provide a method for producing a herbal blend extract of silverweed, hide, milk thistle.

Another object of the present application is to provide a herbal blend extract of Silverweed, Heather, Milk Thistle.

Still another object of the present application is to provide an antioxidant, anti-inflammatory, whitening and moisturizing functional cosmetic composition containing the herb extract of silverweed, hide, milk thistle as an active ingredient.

Other objects and advantages of the present application will become more apparent from the following detailed description taken in conjunction with the appended claims and drawings. The content not described herein is well recognized and inferred by those skilled in the art or similar fields of the present application, and thus description thereof is omitted.

Hereinafter, the content of the present application will be described in detail. In addition, description and embodiment of one embodiment disclosed by this application can be applied also to description and embodiment of other aspect about common matter. In addition, all combinations of the various elements disclosed in the present application are within the scope of the present application. In addition, the scope of the present application is not to be limited by the specific description described below.

In one aspect, in order to achieve the object of the present application provides a method for producing a herbal mixed extract of Silverweed, Heather, Milk Thistle. The production method is a silverweed, the heather, milk thistle is extracted with water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent at 60 ℃ to obtain each of the silverweed, hide, milk thistle extract Making a step; It is characterized in that it comprises a step of mixing each of the silverweed, hide, milk thistle extract obtained in the above step to obtain a mixed extract thereof. As the mixed solvent, water, ethanol, methanol, butylene glycol, propanediol may be used alone or in combination, but the type of solvent is not limited thereto. In addition, the ratio of each solvent may be used in various ways depending on the nature of the extract or the concentration of the target extract. The extraction temperature may be 40 to 80 ℃, specifically 50 to 70 ℃, preferably 60 ℃. The extraction may use an extraction time for 1 day to 4 days. The mixed solvent may be used in a ratio of 1:10 (W / W) to 1: 100 (W / W), but the ratio is not limited thereto. In order to obtain the herb mixture extract by mixing the extracts of each of the silverweed, hide, and milk thistle obtained, each raw material may be mixed in various ratios (W / W), but preferably each extract in the same ratio ( W / W) can be combined to obtain their herbal mixture extract.

In another aspect, the present application provides an antioxidant, anti-inflammatory, whitening, and moisturizing functional cosmetic composition comprising an active ingredient of silverweed, hide, and milk thistle mixed extract. The herb mixture extract may be used in an amount of 0.01% to 20% by weight, preferably 5% to 10% by weight, based on the total weight of the composition. 10 wt% was used in this preparation.

The cosmetic composition of the present application may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, essence, shampoo, surfactant-containing cleansing, tonic, scaling, and the like, but is not limited thereto. More specifically, various formulations such as flexible lotion, nourishing lotion, nutrient latex, nourishing lotion, massage lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, hair essence, rinse It may be prepared by the following preparation method, but the preparation method is not limited thereto.

The present application has an excellent effect to provide an antioxidant, anti-inflammatory, whitening and moisturizing functional cosmetic composition and a method for producing the same, containing the herb extract extract of Silverweed, Heather, Milk Thistle as an active ingredient.

Figure 1 is a graph showing the results of the antioxidant experiment of each extract and herbal mixture extract of the present invention.
Figure 2 is a graph showing the results of the whitening activity of each extract and the herbal mixture extract of the present invention.
Figure 3 is a graph showing the results of the anti-inflammatory test of each extract and herb mixture extract of the present invention.
Figure 4 is a graph showing the results of the moisturizing experiment of each extract and herb mixture extract of the present invention.

Hereinafter, the present invention will be described in more detail through examples according to the present application. However, the following examples of the present application are merely examples of the present application. These examples are intended to illustrate the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention as set forth in the appended claims is not limited by these examples. something to do.

Example 1 Preparation of Silverweed, Heather, Milk Thistle Extract and Herbal Mixture Extract thereof

Silverweed, Heather, Milk Thistle extract of the present invention, each raw material in water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof 1:10 (W / W) to 1: 100 (W / W) Extracted at 60 ° C. for 1 to 4 days at a ratio of to give each silverweed, hide and milk thistle extract.

Each of the obtained silverweed, hide, and milk thistle extract was combined in the same amount (W / W) to obtain their herbal mixture extract.

Experimental Example 1. Antioxidant Experiment

In order to assay the antioxidant activity of each extract and the herbal mixture extract obtained in Example 1 was tested using DCFH-DA (2'7'-dichloro flurescin0diacetate). To this end, skin fibroblasts (HDFn) were suspended in DMEM (10% serum and 1% antibiotics) medium and incubated for 24 hours at 37 ° C., 5% CO ₂ incubator at 1 × 10 ⁵ cells per well. Skin 2% of each extract in fibroblasts (v / v) concentration of 37 ℃, 5% of 1 hour induce the cultures after oxidative stress material in a CO ₂ incubator H ₂ O was treated ₂ 1mM and DCFDA 20M obtained in the above step After the addition was measured by a fluorescence spectrometer (spectrofluorophotomer) under excitation 485nm, emission 535nm conditions. The treated group was measured as the control group without the antioxidant activity was used as a control and the antioxidant activity was calculated by the method of Equation 1 below. All experiments were repeated three times.

Experimental results, as can be seen in Table 1 and Figure 1, the antioxidant inhibition rate of the herbal blend extract than each extract was the highest as 45%.

Experimental Example 2. Whitening Experiment

In order to assay the whitening activity of each of the extracts obtained from Example 1 and the mixed extract of the herb, murine melanoma (B-16 F1) cells were well-separated in 6-well plates with DMEM medium containing an appropriate amount of fetal bovine serum (FBS). After inoculation with 1 × 10 ⁵ cells, the cells were cultured under 5% CO ₂ and 37 ° C. until the cells adhered to the bottom of the well at least about 80%. After incubation, the medium was removed, the sample was replaced with a medium diluted to an appropriate concentration, and then incubated at 5% CO ₂ , 37 ° C. for a predetermined time. The cells from which the medium was removed were washed with PBS (phosphated buffer saline) and treated with trypsin to recover the cells. The recovered cells were counted using a hematocytometer, centrifuged at 5,000 to 10,000 rpm for 10 minutes, and then the supernatant was removed to obtain pellets. The cell pellet was dried at 60 ° C., and then added 100 μl of 1M sodium hydroxide solution containing 10% DMSO or an appropriate amount of cell lysis buffer to obtain intracellular melanin in a 60 ° C. thermostat. Using the solution, the absorbance was measured at 405 nm using a microplate meter to determine the amount of melanin per cell constant or the amount of melanin per protein.

As a result, as shown in Table 2 and Figure 2, the whitening activity (melanin production inhibition rate) of the herbal mixture extract was higher than the respective extracts 43%.

Experimental Example 3. Anti-inflammatory Test

After artificially treating LPS, an inflammation-inducing substance, RAW 264.7 cells were evaluated for their inhibitory effect. Into a 96-well plate to 2 x 10 ⁵ cells per well was incubated for 24 hours at 37 ℃, 5% CO ₂ incubator. After overnight culture (Overnight) was replaced with fresh medium and LPS (1㎍ / ml) and the extract was administered at 2% (v / v) and incubated for 24 hours. After incubation, the supernatant of the medium was taken and centrifuged at 13,000 rpm for 3 minutes to collect only the supernatant, and then reacted 1: 1 with Griess reagent and the absorbance was measured at 570 nm.

As a result, as shown in Figure 3 and Table 3, the herbal mixture extract showed the highest NO production inhibition rate of 40% than each extract.

Experimental Example 4. Moisturizing Experiment

 In general, the evaluation method, which is called the hydration degree of the stratum corneum, that is, the skin moisture content, is mainly evaluated using the electrical properties of the stratum corneum. The most widely used method of evaluating moisture content is by measuring capacitance. Healthy subjects (n = 6, 28-40 years old, 34 years old) were selected to apply cosmetics to the forearm (absorbed for 5 minutes in a 2 cm x 2 cm area) and to evaluate the change in capacitance over time. . In this experiment, the skin capacitance was measured using the moisture measuring device Skin-O-mat (cosmomed, Germany), and the results are expressed as the average of the relative values to the initial values. All experiments were conducted in a space with controlled temperature and humidity.

As a result of the experiment, as shown in Figure 4 and Table 4, the moisture content of 15% was increased when comparing the experimental group coated with the cream containing the mixed extract and the experimental group coated with placebo cream after 2 hours of application. In addition, the moisture content efficacy was not shown in each of the single extracts, but showed a high moisturizing effect when using the herbal mixture extract.

Preparation Example 1. toilet water

The skin lotion containing the herbal mixture extract of Silverweed, Heather, Milk Thistle obtained in Example 1 was prepared as shown in Table 5 below.

Raw material Raw material Content (% by weight) One Inventive Herbal Mixed Extract 10.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

Preparation Example 2. Lotion

The lotion containing the herbal mixture extract of Silverweed, Heather, Milk Thistle obtained in Example 1 was prepared as shown in Table 6.

Raw material Raw material Content (% by weight) One Inventive Herbal Mixed Extract 10.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Solvidan Sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbidate stearate 1.0 7 Lipophilic Monostearine Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

Preparation Example 3. cream

The cream containing the herbal mixture extract of Silverweed, Heather, Milk Thistle obtained in Example 1 was prepared as shown in Table 7.

content Raw material Content (% by weight) One Inventive Herbal Mixed Extract 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Solvanesesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Floating paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

Production Example 4 Essence manufacturing

The essence containing the herbal mixture extract of Silverweed, Heather, Milk Thistle obtained in Example 1 was prepared as shown in Table 8.

content Raw material Content (% by weight) One Inventive Herbal Mixed Extract 10.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

From the above description, those skilled in the art will appreciate that the present application can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present application should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present application.

The present application relates to an antioxidant, anti-inflammatory, whitening, and moisturizing functional cosmetic composition containing a herbal blend extract of silverweed, hide, milk thistle as an active ingredient, and a method for manufacturing the same, in particular, cosmetics of various formulations such as creams, lotions, ointments There is an excellent effect that can be used is a very useful invention in the natural cosmetics industry.

Claims (6)

delete delete delete Silverweed, Heather, Milk Thistle extract containing a combination of antioxidant, anti-inflammatory, whitening and moisturizing functionality as an active ingredient. delete delete

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