KR102029546B1 - Anti-aging and anti-allergic cosmetic compositon comprising the extract of the flower, root, callus and seed of Hibiscus syriacus L. as an active ingredient and preparation method of the same - Google Patents

Abstract

The present application relates to an anti-aging or anti-allergic cosmetic composition and a method for producing the same, characterized in that each of the same ratio (w / w) mixture of each extract of the flowers, roots, callus and seeds of the green light plant as an active ingredient.

Description

Anti-aging and anti-allergic cosmetic composition comprising a mixed extract of the flowers, roots, callus and seeds of rose of sharon as an active ingredient and a method for producing the same {Anti-aging and anti-allergic cosmetic compositon comprising the extract of the flower, root, callus and seed of Hibiscus syriacus L. as an active ingredient and preparation method of the same}

The present application relates to an anti-aging and anti-allergic cosmetic composition and a method for producing the same, containing each of the same ratio mixture of the extract of each site, such as flowers, roots, callus and seeds of the rose of sharon.

Rose of Sharon ( Hibiscus syriacus L.) Native to China and India (India), it is a plant that grows throughout the country and is distributed in East Asia and Europe. The stem is straight, the branches are split, and the height is 2-3m. The leaves are alternately ovate, broad ovate, triangularly shallowly divided, and have coarse teeth on the edge. Hairs on both sides of the leaves. Flowers bloom one by one on the upper side of the branch in August-October, reddish purple or white. Calyx is tubular, divided into 5 branches. 5 petals, attached to the bottom. Surgery is a lot, the operating table is attached to a bunch. Fruits are capsules, round, ovate, and split into five when ripe. There are records that it is used for edible, ornamental and medicinal purposes. The Rose of Sharon is colorful and five petals are attached to each other at the base. It is often white and reddish purple with dark reddish purple inside. Compared to H. hamabo Siebold et Zucc., It is not a native plant but a planting tree, and its leaves are divided with shallow edges, and the flowers are red or white and not yellow.

Skin is a part of the body that is directly exposed to the external environment, and when exposed to excessive ultraviolet rays or contaminants, skin irritation and inflammatory reactions such as erythema, edema, and itching are induced. This skin trouble is not only an aesthetic problem, but also an ancillary pigmentation of the substances produced during the inflammatory process, and an increase in skin aging by promoting the breakdown of skin elastic fibers, and such skin damage is caused by reactive oxygen species, ROS).

Free radicals are oxygen that is in an unstable state completely different from the oxygen we breathe, destroying cells, cutting connective tissue in the dermis, or causing cross-linking, resulting in wrinkles, skin cancer, cell killing, atopic dermatitis, acne, etc. Cause problems. Antioxidants such as superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol remove the free radicals in the human body, but the antioxidant system is inactivated by age, UV, and stress, and the free radicals gradually increase. Causes skin aging.

Inflammation is a physiological response that protects the body from harmful environmental conditions, such as invasion and mechanical damage of foreign objects such as bacteria. The inflammatory response induces the proliferation of several types of multinucleated leukocytes (PMNs), and these inflammatory cells defend the body by releasing various types of proteases and cytokines. However, excessive proteolytic enzyme production of the skin due to excessive inflammatory reactions causes damage to non-cellular connective tissue, and damage to connective tissue reduces skin elasticity and causes wrinkles, as well as regeneration and proliferation of cells. Bad influence

Allergy is a type of hypersensitivity reaction caused by immune imbalance, which occurs when a specific antigen, called allergen, is bound to mast cells or basophils. . The mast cells or basophils activated by the IgE antibody secrete inflammatory cytokines, prostaglandins and histamine, causing vasodilation and inflammatory lesions, causing allergic atopy, asthma, and skin allergies. These allergic diseases are significantly increased incidence and thus the importance of the alleviation and prevention of the disease is highlighted.

Wrinkles of the skin are said to be reduced due to the aging of collagen or photoaging by ultraviolet irradiation and thinning of the skin thickness. Recently, research on skin aging has been progressed to reveal the function of collagen on the skin, and in particular, studies on the prevention of collagen synthesis or reduction, and products using the cosmetic composition for strengthening the skin have been released.

Rose of Sharon contains saponariin, malvalic acid, terculic acid, tannic acid, dihydrosteric acid, mucus, etc., which are known to be contained in ginseng in a large amount. Research is being conducted to utilize them as anti-aging or various functional cosmetic ingredients. Against this background, the present inventors have developed various anti-aging and anti-allergic functional cosmetic compositions which extract, extract, and extract various parts of flowers, roots, callus and seeds thereof, which are collected from Mugunghwa, as their active ingredients. As a result of intensive research efforts, the present application was completed.

There are Republic of Korea Patent No. 10-1564430 and Patent No. 10-1762750 as prior art related to the present invention, but this relates to a cosmetic comprising the extract of Mugunghwa itself, there is no disclosure of its use or mixture composition of other parts of the present invention And is heterogeneous. In addition, there is a Republic of Korea Patent No. 10-1701476, but this relates to a method of preparing the extract and the food containing the extract, the use of the functional cosmetic composition using a mixed extract consisting of the same amount of the extract of each part of the plant of the present invention Is not disclosed or implied in any of the above references.

An object of the present application is to provide a method for producing a mixed extract of the flowers, roots, callus and seeds of Rose of Sharon.

Another object of the present application is to provide a mixed extract of the flowers, roots, callus and seeds of Rose of Sharon.

Another object of the present application is to provide an anti-aging and anti-allergic functional cosmetic composition containing a mixed extract of flowers, roots, callus and seeds of rose of sharon, as an active ingredient.

Other objects and advantages of the present application will become more apparent from the following detailed description taken in conjunction with the appended claims and drawings. Content not described herein is omitted because it can be sufficiently recognized and inferred by those skilled in the art or similar fields of the present application.

Hereinafter, the content of the present application will be described in detail. In addition, description and embodiment of one embodiment disclosed by this application can be applied also to description and embodiment of other aspect about common matter. In addition, all combinations of the various elements disclosed in the present application are within the scope of the present application. In addition, the scope of the present application is not to be limited by the specific description described below.

In order to achieve the object of the present application, in one aspect, there is provided a method for preparing a mixed extract of Rose of Sharon, characterized by obtaining a mixed extract of flowers, roots, callus and seeds of the Rose of Sharon. The manufacturing method is a step of extracting each part of the green plant, that is, flowers, roots, seeds at high temperature using water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof to obtain an extract, and each of the parts Mixing the star extract and the Rose of Sharon callus extract in the same amount of compounding ratio to obtain a mixed extract of the flowers, roots, callus and seeds of Rose of Sharon. It is possible to mix the extract of each plant of the greenery plant and the extract of the green leaf callus extract using various ratios (W / W) of the solvent, but it may be preferably mixed in the same amount (W / W). In addition, the extraction temperature may be used in the range of 50 to 90 ℃ and can be extracted for 1 to 4 days, preferably at 60 ℃ 1 to 4 days. The mixed solvent may be used in a ratio of 1:10 to 1: 100, but a usable ratio is not limited thereto.

Anti-aging is a concept that includes anti-inflammatory, anti-wrinkle, antioxidant in the skin can be measured by the amount of reduction of each NO production, the amount of collagen production, the amount of ROS production, but the biomarkers and measuring methods used are not limited thereto.

In another aspect, the present application provides a method for preparing an anti-aging or anti-allergic cosmetic composition using a mixed extract of flowers, roots, callus and seeds of rose of sharon as an active ingredient. The cosmetic composition of the present application may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, essence, shampoo, surfactant-containing cleansing, tonic, scaling, and the like, but is not limited thereto. More specifically, it may be prepared in a formulation such as softening lotion, nourishing lotion, nutrient latex, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, hair essence, rinse.

The present application has an excellent effect of providing a cosmetic composition and a method for producing the same, which have significantly increased anti-aging or anti-allergic function as an active ingredient of a mixed extract of flowers, roots, callus and seeds of Rose of Sharon.

1 is a graph showing the anti-inflammatory efficacy of the extract of Rose of Sharon.
Figure 2 is a graph showing the anti-inflammatory efficacy of the rose of sharon flowers.
Figure 3 is a graph showing the anti-inflammatory efficacy of the green light callus extract.
Figure 4 is a graph showing the anti-inflammatory efficacy of the Rose of Sharon root extract.
5 is a graph showing the collagen production proliferation efficacy of the green light callus extract.
Figure 6 is a graph showing the collagen production proliferation efficacy of Rose of Sharon flower extract.
Figure 7 is a graph showing the collagen production proliferation efficacy of Rose of Sharon root extract.
8 is a graph showing the collagen production proliferation efficacy of the Rose of Sharon seed extract.
9 is a graph showing the antioxidant efficacy of the rose of sharon flower extract.
10 is a graph showing the antioxidant efficacy of the green light callus extract.
11 is a graph showing the antioxidant efficacy of the Rose of Sharon seed extract.
12 is a graph showing the antioxidant efficacy of the Rose of Sharon root extract.
Figure 13 is a graph showing the anti-allergic effect of Rose of Sharon root extract.
Figure 14 is a graph showing the anti-allergic effect of Rose of Sharon flower extract.
Figure 15 is a graph showing the anti-allergic effect of Rose of Sharon seed extract.
Figure 16 is a graph showing the anti-allergic efficacy of Rose of Sharon callus extract.
17 is a graph showing the antioxidant efficacy of each extract of the same ratio (w / w) of the flowers, roots, callus and seeds of Rose of Sharon.
Figure 18 is a graph showing the anti-allergic efficacy of each extract of the same ratio (w / w) of the flowers, roots, callus and seeds of Rose of Sharon.
Figure 19 is a graph showing the collagen production proliferation efficacy of each of the same ratio (w / w) mixed extract of the flowers, roots, callus and seeds of Rose of Sharon.
20 is a graph showing the anti-inflammatory effect of each equivalent ratio (w / w) mixed extract of flowers, roots, callus and seeds of Rose of Sharon.

Hereinafter, the present invention will be described in more detail through examples according to the present application. However, the following examples of the present application are merely examples of the present application. These examples are intended to illustrate the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention as set forth in the appended claims is not limited by these examples. something to do.

Example 1 Preparation of Flowers, Roots, Seeds and Callus Extracts of the Rose of Sharon

The extract of the flowers, roots, and seeds of the green light plants of the present invention is 1:10 (W / W) to 1: in the water, ethanol, methanol, butylene glycol, propanediol or a mixed solvent thereof. Obtained by extraction at 60 ° C. for 1 to 4 days at a rate of 100 (W / W).

The green light callus extract of the present invention was obtained by the following method. First, the leaves of Rose of Sharon are seeded on a medium and incubated under dark conditions at a temperature of 20-27 ° C. (preferably 22 ° C.) and 65-75% (preferably 55-60%) humidity to induce germination. The leaves of Rose of Sharon were cut and inoculated into MS basal medium containing IBA (indole-3-butyric acid), sucrose (Sucrose) and agar (Agar), and then cultured at 23-27 ° C to induce callus. Then, the cultured green light callus was propagated through subculture, and the callus culture of the grown green light plant was harvested to remove moisture, and then dried at a temperature condition of 45-55 ° C. Purified water (or solvent) was added to the dried green light calli culture, and hot water (or solvent) was extracted to obtain a light green callus extract.

Extracts and callus extracts of each part of the flowers, roots, and seeds of the rose of sharon obtained in each step were combined in equal amounts (W / W) to obtain a mixed extract thereof.

Experimental Example 1. Determination of anti-inflammatory effect of flowers, roots, seeds and callus extracts

In order to measure the anti-inflammatory effect of the flowers, roots, seeds and callus extract of the plant of the present invention, it was evaluated whether the RAW 264.7 cells were treated with LPS, an inflammation-inducing substance, to have an anti-inflammatory effect. Into a 96-well plate to 2 x 10 ⁵ cells per well was incubated for 24 hours at 37 ℃, 5% CO2 incubator. After overnight culture, the cells were replaced with fresh medium, and LPS (1ug / ml) and extracts were administered at different concentrations. After incubation, the culture medium was collected, centrifuged at 13,000 rpm for 3 minutes to collect only the supernatant, reacted with Griess reagent 1: 1, and absorbance was measured at 570 nm.

As a result, at the LPS concentration of 2%, the anti-inflammatory effect of the extract of each part of the green light plants could be measured (Figs. 1 to 4), and specifically, the extracts of the green light seeds, flowers, callus, and roots were 23%, respectively. The anti-inflammatory effect of inhibition of nitric oxide (NO) production of 19%, 16% and 11% was confirmed. It was confirmed that the anti-inflammatory effect of the Rose of Sharon seed extract of each part of the extract of Rose of Sharon plant according to the present invention is the most excellent.

Experimental Example  2. the flowers, roots, seeds, and Callus  Determination of Collagen Production Proliferation Effect of Extracts

Skin fibroblasts (HDFn) were inoculated in 0.3 mL of culture medium at a concentration of 5 × 10 ⁴ cells / well in 48 well plates and cultured at 37 ° C. and 5% CO ₂ . After 24 hours, the extract of each rose of sharon was incubated for 24 hours with fresh medium containing the concentration. After 24 hours of incubation, the culture solution was collected and the amount of C-terminus of the collagen precursor was measured using an enzymes-linked immunoassay kit (Takara). In addition, using a collagen solution 640 ng / mL to obtain a standard curve between the collagen concentration 0 ng-640 ng to calculate the amount of collagen in the medium.

As a result of the calculation, it was possible to confirm the collagen production and proliferation efficacy of each extract of the plant of the plant of the green plant at the concentration of 2% (Figs. 5 to 8), and in particular, each extract of the green leaf callus, flower, and seed was 20% and 11, respectively. Collagen production proliferation efficacy of 5% and 5% was confirmed. That is, it was confirmed that the most effective collagen production proliferation effect of the green leaf callus extract of each region extract of the green plant.

Experimental Example  3. the flowers, roots, seeds, and Callus  Antioxidant Activity of Extracts

DCFH-DA (2'7'-dichloro flurescin0diacetate) was used to assay the antioxidant activity of the flowers, roots, seeds and callus extract of the plant of the present invention. To this end, skin fibroblasts (HDFn) were suspended in DMEM (10% serum and 1% antibiotics) medium and incubated for 24 hours at 37 ° C., 5% CO ₂ incubator at 1 × 10 ⁵ cells per well. A skin fibroblast Hibiscus plants 37 ℃ to handle each part extracts at different concentrations to, 5% CO 1 time of incubation of induced oxidative stress substances H ₂ O ₂ 1mM and DCFH-DA 20M in the _second culture medium obtained in the above step After addition, excitation is 485nm and emission is 535nm

Measurement was performed using a spectrofluorophotomer. The treatment group was measured as the control group without the addition of the antioxidant activity was used as a control and the antioxidant activity was calculated by the method of the following equation (1). All experiments were repeated three times.

[Equation 1]

Reactive Oxygen Species Inhibition Rate (%) = Control Group OD-Sample Treatment Group OD / Control Group OD X 100

As a result of the experiment, at the concentration of 2% extract, the antioxidant effects of the extracts of each part of the rose of sharon were confirmed (Figs. 9 to 12). Among them, the extracts of the rose of sharon and the callus were 20% and 15%, respectively. there was. According to the present invention, it was confirmed that the antioxidant effect of the rose of sharon flower extract was the best among the extracts of each part of the rose of sharon plant.

Experimental Example 4. Determination of anti-allergic efficacy of flowers, roots, seeds and callus extracts of Rose of Sharon

24-well plate after suspending RBL-2H3 cells in DMEM (Dulbecco's modified Eagle's medium) containing 10% FBS to assay the anti-allergic effect of each part, flower, root, seed and callus extract of the plant Each well was treated to enter 2 x 10 ⁵ cells. Each well was sensitized with 100 ng / mL mouse monoclonal IgE and incubated in a 5% CO ₂ incubator for 24 hours. The cells were rinsed using 500 μl of Tyrode buffer per well and treated with mixed extracts according to concentrations. The cells were then incubated in 37 ° C., 5% CO ₂ incubator for 15 minutes, and treated with DNP-BSA 100ng / ml for 1 hour. 50 μl of substrate (1 mM p-nitrophenyl-N-acetyl- ㅯ -D-glucosaminide) was treated in the supernatant, and then incubated for 1 hour at 37 ° C. in a 5% CO 2 incubator, and the absorbance was measured at 405 nm using an ELISA reader. It was.

As a result, the anti-allergic effect of the extract of each rose of sharon was confirmed at 2% of the extract concentration (Figs. 13 to 16). Among them, the extract of the root of rose root showed 27% of the anti-allergic effect, and the anti-allergic effect was the most. It confirmed that it was excellent.

Experimental Example 5. Determination of anti-inflammatory, collagen-producing proliferation, antioxidant and anti-allergic efficacy of each equivalent ratio complex of flowers, roots, callus and seeds of Rose of Sharon plant

The mixed extracts of the flowers, roots, callus and seeds of rose of sharon obtained in Example 1 were evaluated for each function using the anti-inflammatory, collagen proliferation, antioxidant and anti-allergic efficacy measurement methods described in Experimental Examples 1 to 4. Experimental results, the mixed extract of the flowers, roots, callus and seeds of Rose of Sharon has 25% antioxidant activity (Fig. 17), 20% anti-allergic effect (Fig. 18), 16% collagen growth efficiency (Fig. 19) compared to the control group , Anti-inflammatory efficacy of 21% (Fig. 20) was confirmed, respectively.

Hereinafter, various cosmetic formulations are preferred for use in the present invention, but the scope of the present invention is not limited to the disclosed formulations.

Preparation Example 1. toilet water

The present invention, a lotion containing a mixed extract (hereinafter, a mixed extract) of the flowers, roots, callus, and seeds of the rose of sharon obtained in Example 1 was prepared as shown in Table 1 below.

Raw material Raw material Content (% by weight) One The present invention mixed extract 10.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Remaining amount

Preparation Example 2. Lotion

The present invention obtained a lotion containing a mixed extract (hereinafter, a mixed extract) of the flowers, roots, callus and seeds of the present rose of sharon obtained in Example 1 was prepared as shown in Table 2.

Raw material Raw material Content (% by weight) One The present invention mixed extract 10.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solvidan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbidate stearate 1.0 7 Lipophilic Monostearine Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Remaining amount

Preparation Example 3. cream

The present invention, a cream containing a mixed extract of the flower, root, callus, and seed of the present invention obtained from Example 1 (hereinafter, referred to as a mixed extract) was prepared as shown in Table 3 below.

content Raw material Content (% by weight) One The present invention mixed extract 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Solvanesesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Floating paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglycerides 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Remaining amount

Preparation Example 4. Essence manufacturer

The essence containing the mixed extract of the flower, root, callus and seed of the present invention obtained in Example 1 (hereinafter, mixed extract) was prepared as shown in Table 4 below.

content Raw material Content (% by weight) One The present invention mixed extract 10.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Remaining amount

From the above description, those skilled in the art will appreciate that the present application can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the embodiments described above are exemplary in all respects and not limiting. The scope of the present application should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present application.

The present invention relates to an anti-aging or anti-allergic cosmetic composition comprising a mixed extract of each extract of the flowers, roots, callus and seeds of the rose of sharon as an active ingredient and a method for producing the same, in particular cosmetics of various formulations such as creams, lotions, ointments There is an excellent effect that can be used is a very useful invention in the natural cosmetics industry.

Claims (6)

Extracting the flowers, roots, and seeds of each part of rose of sharon from water, ethanol, methanol, butylene glycol, propanediol, or a mixed solvent thereof at 60 ° C. to obtain an extract for each site;
The green light mixture prepared by mixing each part of the extract obtained in the step and the green light callus extract in each equivalent ratio (w / w) to obtain a mixed extract (complex) of the flowers, roots, seeds and callus of the green light extract
delete delete A cosmetic composition for improving wrinkles, comprising the mixed extract of claim 1 as an active ingredient
A cosmetic composition for improving allergy containing the extract of Mugunghwa as an active ingredient
The cosmetic composition for improving wrinkles and allergy according to claim 4 or 5, wherein the cosmetic composition is any one selected from a lotion, lotion, cream and essence.

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