KR101070070B1 - Cosmetic composition for preventing skin aging - Google Patents

Abstract

The present invention relates to a cosmetic composition having an anti-aging effect, and more particularly, a herbal medicine mixture (hereinafter referred to as 'hongokgami') mixed with white paper, kwakhyang, sculpture, and clove extract is mixed with chrysanthemum and licorice extract. By containing a herbal mixture (hereinafter referred to as chrysanthemum sound), the present invention relates to a cosmetic composition for preventing skin aging, which is excellent in cell proliferation activity, antioxidant effect, anti-inflammatory effect, and anti-aging activity in skin.

The cosmetic composition according to the present invention can maximize the anti-inflammatory effect by synergistic effect by simultaneously applying two kinds of herbal mixture of hongokgami and chrysanthemum, as a safe substance without irritation to the skin, lotion, essence, lotion , Creams, packs, gels, ointments, patches, or sprays.

Skin aging prevention, cosmetics, cell proliferation activity, antioxidant, anti-inflammatory, anti aging

Description

Cosmetic composition for preventing skin aging {COSMETIC COMPOSITION FOR PREVENTING SKIN AGING}

Figure 1 shows the results of measuring the cell proliferation activity of ruby gill gill. S10 is a positive control, S0 is a negative control, S1 is an experimental control.

2 is a result of measuring the cell proliferation activity effect of chrysanthemum. S10 is a positive control, S0 is a negative control, S1 is an experimental control.

Figure 3 shows the antioxidant effect of the ruby scavenger, showing the free radical scavenging ability by concentration.

Figure 4 shows the antioxidant effect of chrysanthemum sound, showing the free radical scavenging ability by concentration.

5 is a graph showing the results of measuring the inhibitory effect of PGE2 production at the cellular level of chalcopyrite and chrysanthemum and the synergistic effect of the application of both materials simultaneously.

The present invention relates to a cosmetic composition having an anti-aging effect of skin, and more particularly to a skin anti-aging cosmetic composition having excellent cell proliferation activity, antioxidant effect, anti-inflammatory effect, and anti-aging activity in skin, ruby Simultaneous application of two kinds of herbal mixtures of acid and chrysanthemum can maximize the anti-inflammatory effect by synergistic effect.It is a safe substance without skin irritation, lotion, essence, lotion, cream, pack, gel, ointment It relates to a cosmetic composition for preventing skin aging that can be used in the form of a patch, patch, or spray.

The skin of the human body is a part directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, free radicals or free radicals are generated, and erythema, edema, rashes, tingling, and itching by the generated free radicals or free radicals Skin irritation and inflammatory reactions in the back are induced. Skin troubles caused by these causes are not only aesthetic problems, but also substances produced during the inflammatory process may cause pigmentation of the skin and accelerate the breakdown of skin elastic fibers, thereby affecting the increase of skin wrinkles. It is known to lead to skin aging.

As the skin ages, keratinization and epidermal cell fusion rate gradually slows down, the epidermal cell layer becomes thinner, the stratum corneum becomes thicker, and the boundary line with the dermal layer becomes smoother. In the dermis, fibroblasts age to decrease the ability to produce fibers and substrates, and skin cells rapidly increase peroxide levels in cells as they age, and are susceptible to various pollutants and stress. The components of cells and cell membranes are changed to peroxides, protein damage occurs, and the cells are unable to perform their functions smoothly, which lowers the metabolic activity of the cells, and wrinkles are caused by these functional and structural damages. Further promoted. Preventing all steps involved in this mechanism of skin aging can fundamentally prevent skin aging.

Accordingly, efforts to find a material that can protect against aging have been ongoing from the past to the present, and researches to search for new raw materials with antioxidant activity are being actively conducted at home and abroad. Most of the substances studied under the name of anti-aging agents are antioxidants such as vitamin C and E, thiol group N-acetyl-L-cysteine, S-allyl-L-cysteine, etc. Antioxidants include beta-carotene, curcumin, ellagic acid, and fumaric acid. Recently, carnosine, an antioxidant present in muscle, has been reported to maintain cell morphology during aging, and PBN (α-phenyl-Nt-butyl nitrone) has long life expectancy in cells and experimental animals. Although it has been reported to be effective, not much has been reported as an anti-aging substance.

However, aging is not only caused by oxidation but is known to be affected by numerous factors such as telomeres, cell replication ability, gene damage and recovery ability, and thus, there have been many difficulties in discovering natural products and drugs.

In order to solve the problems of the prior art as described above, the present invention activates the skin cells without causing other irritation or side effects on the skin, cell proliferation activity, antioxidant effect, anti-inflammatory effect, and synergistic effect in the skin An object of the present invention is to provide a cosmetic composition for preventing skin aging that is excellent and particularly excellent in anti-aging activity.

The present invention provides a cosmetic composition for preventing skin aging, comprising a herbal mixture consisting of white paper, squat, sculpture, and clove extract, and a herbal mixture consisting of chrysanthemum and licorice extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present inventors studied various substances to search for excellent anti-aging efficacy substances from natural natural products, which have been conventionally used as herbal medicines, and as a result, herbal mixtures containing white paper, kwakhyang, sculptor, and clove extract (hereinafter ' Mixed extract of herbal mixture mixed with chrysanthemum and licorice extract (hereinafter referred to as chrysanthemum sound) has excellent cell proliferation, antioxidant, anti-inflammatory and synergistic effects in skin This invention was completed.

The present invention relates to a cosmetic composition excellent in human safety and excellent skin anti-aging activity, activating cell proliferation, excellent free radical scavenging activity, and 'hongoksangami' and 'chrysanthemum' to excellently inhibit the production of PGE2 It is characterized by containing a mixed extract called '.

'Hongoksangami' provided in the present invention is obtained by mixing white paper, wagyang, engraver, and clove extract.

Angelica dahurica is a biennial or triennial herb of the dicotyledonous plant Asteraceae, also known as Daebyeong, Heungan Baekji, Dokbyeong, Copperdae, and Gumbaegi. Grows in a valley stream. There is no hair throughout the grass, the roots are very thick, and the roots are much lower. Stems stand straight and 1 ~ 2m high. The leaves are divided into 3 ~ 2 times of pinnate leaf with 3 pieces, and the split pieces are oval or lanceolate with sharp ends and uneven teeth. White flowers bloom in June-August, with 20-40 inflorescences forming a double inflorescence. No guns, small guns are thin and small. Corolla is small with 5 petals, 5 stamens, and 1 ovary under calyx. Fruit is branched, oval, winged and ripens in October. Young leaves are edible. Herbal medicines made by drying the root is called white paper, and is prescribed for sweating, soothing, pain, blood, cold, headache, pain, toothache. It is distributed in Korea, Japan, northeastern China and eastern Siberia.

The Gwakhyang (Teucrium veronicoides) is a perennial herb of the dicotyledon and moss plant in the mountain. It is 20-30cm in height. Hairs 1 ~ 2mm long throughout the grass. The creeper stands upright and branches. Leaves are oval or broad oval, somewhat blunt at end. Leaves are 2.5-4cm long, 1.5-2.5cm wide, with serrates, and petioles are 1-2cm long. Flowers bloom in light blue color in July-August, which are sparsely inclined to one side. The length of the flower is about 8 mm, and the length of the inflorescence is 4 to 8 cm. Pau shorter than broad calyx. Calyx is sparsely sparse at the top and faces downward when ripe. Fruits are branched, round, glossy, 1.5mm long. It is distributed in Jeju Island and North Hamgyong Province.

The sculptor (Gleditsia sinensis) is a deciduous tree of the dicotyledonous rosewood legume native to China. The spines are large, fusiform, more than 10 cm long. A leaf is a pinnate leaf composed of 3 to 6 pairs of small leaves that are shifted. Small leaves are long oval or lanceolate, narrow at both ends, 1 ~ 9cm long, 5 ~ 35cm butterfly. The pod is flat, 20cm long, 3cm butterfly, and spicy without splitting. Flowers bloom pale yellow and white in June and hang on inflorescences. The fruit ripens in October as a berry. The thorn is called a sculptor and is used for boils and boils. Overwintering is also possible in central Korea.

The clove (Eugenia aromaticum) is an evergreen sub-tree of the dicotyledonous plated cactus plated sheep and is native to the Moloccan Islands. The stem is 4-7m high. The leaves are opposite, fragrant, and lanceolate. Flowers are white, gathered at the ends of branches, and 4 petals. There are many surgeries and one pistil. Fruits are nucleus, black red. Collecting and drying flower buds before they bloom is called cloves or sperm (丁字). The bud is shaped like a nail and has a fragrance, so it is called clove. The English clove is also derived from the French clou. It was already known in China around the third century BC and in the Middle Ages in Rome and Europe. Until the 16th century, the Portuguese discovered the plant in the Molucca Islands, it was unknown, and was originally a portuguese monopoly, but it was brought to the Netherlands. It is very fragrant, so use it as it is or as a powder and use clove oil extracted with water or steam. It is used for food, medicine, and preservatives, or as a painkiller in dentistry, including seizures. Cloves are low in yield, so they use not only buds but also flower beds and fruits.

'Chrysanthemum' mixture provided in the present invention is obtained by mixing chrysanthemum and licorice extract.

Chrysanthemum (Chrysanthemum morifolium) is a perennial plant of the dicotyledon plant Campanula Asteraceae, also called chrysanthemum. Chrysanthemum is widely grown for ornamental purposes, and there are many horticultural varieties. The base of the stem is woody about 1m in height, and the leaves are shifted and split into feathers. The flower is a conical flower, which blooms at the end of the stem, in the middle, ornamental flower and the periphery of snow flower. Snowshoes are single shoes with pistils and coronary flowers are bisexuals with both cancer and surgery. The flowers vary depending on the varieties, such as yellow, white, red, and purple, and their size and shape also vary depending on the variety. Depending on the diameter of the flower is more than 18cm in large ring, 9cm or more in middle ring, less than small ring is called varieties depending on the shape of the petals.

The licorice (Glycyrrhiza uralensis) is a perennial herb of the dicotyledonous plant Rosaceae. The roots are reddish brown deep into the ground, the stems are gathered, and grow straight about 1m. In addition, white hairs are dense, appearing as off-white, and preemption is scattered. The leaves are shifted and oddly littered leaf. Small leaves 7-17 each, egg-shaped, pointed end. Small leaves are 2 ~ 5cm long, 1 ~ 3cm butterfly, white hairs and dots on both sides, and no sawtooth. Flowers bloom in July-August, 1.4-2.5cm long, purple, and hanged on the axilla as a total inflorescence. The pod is thin and long like a line and bent like a bow and contains 6 to 8 seeds. The root is sweet, so use it as a sweetener or herbal medicine. Siberian acid (G. glabra var. Glandulifera) and Spanish acid (G. glabra). A similar species is G. pallidiflora. It is less sweet, unlike licorice, and is not used for medicinal purposes. The hair is small, the fruit is oval, and the tip is sharp. It is distributed in northeastern China, Siberia and Mongolia.

Various herbal ingredients in the mixture of 'hongoksangami' and 'chrysanthemum' provided by the present invention may be extracted by a conventional extraction method, and the type and content of the extraction solvent may be arbitrarily selected according to the purpose. Preferably, the extraction solvent is one pure solvent selected from the group consisting of purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, ethyl acetate, glycerin, propylene glycol and butylene glycol Two or more are combined solvents, and may be used in an amount of 1 to 20 times the total volume of the herbal medicine.

The mixture of 'hongokgami' and 'chrysanthemum' provided by the present invention activates cell proliferation, has excellent free radical scavenging activity, and has the effect of inhibiting prostaglandin production of the cellular inflammatory response, and particularly inhibits the production of prostaglandin E2. Excellent efficacy. In addition, the simultaneous application of 'hongoksangami' and 'chrysanthemum sound' shows much higher inhibition of prostaglandin than a single application, and the two mixtures can be applied simultaneously to maximize anti-inflammatory effects due to synergistic effects. In this case, it is preferable to use hongoksan kami and chrysanthemum in a weight ratio of 10:90 to 90:10.

The cosmetic composition having an anti-aging effect according to the present invention is not particularly limited in its formulation, but may be prepared, for example, in a formulation such as lotion, essence, cream, pack, gel, lotion, ointment, patch or spray. have.

The content of the mixture in the formulation may contain from 0.0001 to 30% by weight, preferably 0.001 to 20% by weight relative to the total weight of the composition. If the content of the mixture is less than 0.0001% by weight relative to the total weight, it is difficult to obtain the desired effect in the present invention, when more than 30% by weight may cause a problem in the formulation stability.

Hereinafter, the present invention will be described in detail with reference to Preparation Examples and Examples. However, the production examples or embodiments according to the present invention can be modified in various other forms, the scope of the present invention is not limited to the examples described below.

Preparation Example 1 Preparation of a Mixture of 'Hongoksangami'

Firstly, white paper, kwak, sculptor and cloves are completely dried, mixed with dry weight of white paper 60 ~ 80g, 20 ~ 40g kwak, 20 ~ 40g flakes, and 60 ~ 80g clove, respectively, and then distilled water, ethanol, 10-20 times by volume of one or more solvents selected from the group consisting of methanol, butanol, ethyl acetate, propanol, glycerin, hydrous butylene glycol, and hydrous propylene glycol were prepared as shown in Table 1 below. Extraction method was prepared by adding the herbal ingredients prepared in the selected solvent, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with a 300 mesh filter cloth, and the filtrate was left at 4-15 ℃ for 7 days to ripen, and then Whatman filter paper The mixture was prepared by filtration with ruby ruby.

division Extraction solvent Volume (kg) Yield (kg) Preparation Example 1 Distilled water 5 3.5 Preparation Example 2 70% ethanol 6 4.1 Preparation Example 3 70% methanol 6 4.2 Preparation Example 4 70% Butanol 6 4.2 Preparation Example 5 Ethyl acetate 5 3.6 Preparation Example 6 Propanol 5 3.7 Preparation Example 7 glycerin 5 3.6 Preparation Example 8 Butylene glycol 4 2.8 Preparation Example 9 Propylene glycol 4 2.8 Preparation Example 10 30% water butylene glycol 4 2.7 Preparation Example 11 30% water propylene glycol 4 2.6

Preparation Example 2 Preparation of a Chrysanthemum Sound Mixture

After completely drying chrysanthemum and licorice in the same manner as in Preparation Example 1, mixed with 200g of chrysanthemum and 2g of licorice and put into a solvent as shown in Table 2 below, extracted by boiling for 12 hours in an extractor with a cooling condenser 300 mesh filter cloth The mixture was filtered, and the filtrate was left to mature at 4-15 ° C. for 7 days, followed by filtration through Whatman filter paper to prepare a “chrysanthemum” mixture.

division Extraction solvent Volume (kg) Yield (kg) Preparation Example 12 Distilled water 3 2.2 Preparation Example 13 70% ethanol 4 2.9 Preparation Example 14 70% methanol 4 3.0 Preparation Example 15 70% Butanol 4 3.0 Preparation Example 16 Ethyl acetate 3 2.3 Preparation Example 17 Propanol 3 2.1 Preparation Example 18 glycerin 3 2.1 Preparation Example 19 Butylene glycol 4 3.2 Preparation Example 20 Propylene glycol 4 3.1 Preparation Example 21 30% water butylene glycol 4 3.1 Preparation Example 22 30% water propylene glycol 4 2.9

Experimental Example 1 Measurement of Cell Proliferation Activity

MTT reduction method by culturing human fibroblasts in order to measure the effect on the cell proliferation activity and toxicity of the cosmetic active ingredient material of the mixture of 'hongoksangami' and 'chrysanthemum' obtained according to Preparation Examples 1 and 2 of the present invention Relative comparison of [3 (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide reduction method] and extraction of formazan product with isopropyl alcohol to measure absorbance at 570 nm It was. The positive control group was used as a medium containing 10% FBS (fetal bovine serum), the medium without FBS was used as a negative control group, and the culture medium without the sample was used as the experimental control group. Experiment was performed using the active ingredient material obtained in 1,3-butylene glycol: water (30:70) in the solvent of Preparation Example, using the following formula 1 to calculate the viability of the cell numerical value in Table 3 Indicated.

matter Concentration (μg / ml) Cell proliferation (%) Ruby 0.1 121.7 One 129.6 10 123.5 100 128.5 1000 131.3 Chrysanthemum 0.1 115.0 One 118.1 10 118.9 100 126.9 1000 127.7 10000 144.8 * Number of replicates = 5

As shown in Table 3, 'hongoksangami' and 'chrysanthemum' mixed extract was found to promote cell proliferation up to 131.3%, 144,8% from the minimum treatment concentration compared to the control, from these results The mixed extract according to the present invention was confirmed that the cell proliferation is excellent, and there is no cytotoxicity.

Experimental Example 2 Measurement of Antioxidant Effect

A free radical scavenging activity test was conducted to investigate the antioxidant effect of the cosmetic active ingredient materials of the mixed mixture of 'hongoksangami' and 'chrysanthemum' obtained according to the preparation example of the present invention. The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), which is a stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl, Sigma) reagent was used. To 1 ml of 0.2 mM DPPH solution (Methanol for Blank), the mixed extract prepared in Preparation Example 1 or 2 was prepared at various concentrations, and each of them was added and mixed with 2 ml, and left at room temperature for 10 minutes. An experimental group for measuring absorbance at 525 nm was set, and the control group was set to the case of using purified water. After measuring the absorbance for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below, and the results are shown in Table 4 below.

matter Concentration (μg / ml) Free radical scavenging rate (%) SC ₅₀ (%) Ruby 0.1 2.7 0.34 One 3.3 10 15.9 100 22.8 1000 42.1 10000 65.6 Chrysanthemum 0.1 5.0 0.08 One 4.2 10 19.4 100 32.5 1000 55.2 10000 70.1 * Number of replicates = 5

In Table 4, SC50 is a concentration of the sample required to achieve 50% free radical scavenging ratio, which is a value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio. Peony extracts are usually 15.51% for SC50, 4.21% for sewage extracts and 14.40% for golden root extracts. As can be seen through the results shown in Table 4, the 'hongokgami' and 'chrysanthemum' mixed extract obtained by the preparation example 1 or 2 according to the present invention is a component material having different free radical scavenging ability It can be confirmed that it is relatively excellent compared to.

Experimental Example 3: Measurement of PGE2 Inhibition Effect and Synergy Effect

The following experiment was conducted to investigate the inhibitory effect of PGE2 production by the mixture of 'hongoksangami' and 'chrysanthemum' [Ref .: Analytical Biochemistry, 1999, 271, 18-28].

First, the fibroblasts isolated from human epidermal tissue were subcultured several times, and then 1 to 105 cells were placed in 96-well plates and incubated in 37 ° C. and 5% (v / v) CO 2 incubator for 24 hours. The mixture of 'hongokgami' and 'chrysanthemum' mixed extracts to be cultured and evaluated for 2 hours by replacing with a medium containing no FBS (fetal bovine serum) was treated at the concentrations shown in Table 5 below at 37 ° C, 5% (v / v) incubated for 2 hours in a CO2 incubator. In addition, the two extracts were treated at the concentrations shown in Table 5 to observe the synergistic effect upon simultaneous application. After treatment with calcium ionophore A23187 and arachidonic acid at 50 μM for 5 minutes as a PGE2 stimulator, dodecyltrimethylammonium bromide was added at a final concentration of 0.25% (w / v) for 10 minutes. The cells were hemolyzed by treatment. 50 μl of the supernatant was added to a plate coated with goat-anti mouse Ig, and the PGE2 standard was also changed at different concentrations (20, 40, 80, 160, 240, 320, 480, 640 pg / 50). 50 μl each was added. 50 μl of PGE2 antibody and PGE2 conjugate were added thereto, followed by reaction at room temperature for 2 hours using a plate shaker. The medium and the buffer were removed, and washed four times. 150 μl of 3,3 ', 5,5'-tetramethylbenzidine (TMB) / hydrogen peroxide was added to the enzyme substrate and reacted with a shaker for 30 minutes. Sulfuric acid) was added and the absorbance was measured and quantified by ELISA reader 450 nm to determine the PGE2 inhibitory effect of the extract. The results are shown in Table 5 below. Inhibition rate of Table 5 was calculated based on the difference in the amount of PGE2 production of skin fibroblasts not treated with arachidonic acid and fibroblasts treated with arachidonic acid.

sample Concentration (μg / ml) PGE2 (pg / 10 ⁵ cells) * Inhibition rate (%) Negative control group - 8 - Positive control (+ arachidonic acid) - 307 - Ruby 100 307 0 1000 320 -4.3 10000 129 59.5 Chrysanthemum 100 293 4.7 1000 307 0.0 10000 170 45.8 Hongoksangami + Chrysanthemum 100 111 65.6 1000 69 79.6 * Number of replicates = 5

Table 5 shows that "hongoksangami" has an anti-inflammatory effect of 59.5% at 10000㎍ / ㎖, and "Chrysanthemum sound" also inhibits the production of PGE2 by the stimulation source up to 45.8% at 10000㎍ / ㎖. The simultaneous application of hongoksangami and chrysanthemum sound resulted in 65.6% of inhibitory effect at 100µg / ml concentration, which did not show anti-inflammatory effect. Indicated.

Therefore, the 'hongoksangami' and 'chrysanthemum' mixed extract through the above test shows a much higher anti-inflammatory effect than when applied together, it can be surely synergistic effect when the two extracts are applied at the same time.

Experimental Example 4: In vivo anti-inflammatory effect measurement

In order to investigate the anti-inflammatory effects of mixed mixtures of 'hongoksangami' and 'chrysanthemum' in animal experiments, mouse ear swelling test (MEST) [References: A. Crummey, GP Harper, EA Boyle and FR Mangan. Inhibition of arachidonic acid-induced ear edema as a model for assessing topical anti- inflammatory compound. Agents and Actions 1987, 20, 69-76.

Twenty-one hairless mice were divided into five groups, each of which was divided into five groups, whereby a mixture of ruby antagonist and chrysanthemum sound was dissolved in ethanol at 0.5% (v / v) and 1.0% (v / v), respectively. Test group and ethanol-only control group.

Both ears of each group of mice were washed with ethanol prior to sample application, and then the average thickness of the ears of the mice was measured using a micrometer. 20 μl of each sample was applied to the test group, 20 μl of ethanol was applied to the control group, and after 1 hour, ethanol was applied to the left ear and arachidonic acid to the right ear of each mouse except the control group. After 1 hour of application, the thickness of both ears was repeated three times using a micrometer to measure the degree of edema of the ears by arachidonic acid.

The left ear of the mice not coated with arachidonic acid was used as a control to determine whether the test group induced inflammation, and showed no difference compared to before application of the sample.

The anti-inflammatory effect of the mixed mixture of 'hongoksangami' and 'chrysanthemum' was determined based on the degree of edema inhibition based on the ethanol-only control, and the results are shown in Table 6.

division Concentration (% by volume) Ear thickness (㎛) Inhibition Rate (%) Before arachidonic acid treatment After arachidonic acid treatment Thickness change Ruby 0.5 325 536 211 29.2 1.0 331 517 186 37.6 Chrysanthemum 0.5 318 523 205 31.2 1.0 324 512 188 36.9 Control group - 328 626 298 -

※ Inhibition Rate (%) = (A-B) / A × 100                     

A: average thickness change of control ear

    (Thickness after arachidonic acid treatment-thickness before arachidonic acid treatment)

B: average thickness change of test group ears

    (Thickness after arachidonic acid treatment-thickness before arachidonic acid treatment)

As shown in the results of Table 6, the test group containing a mixture of 'hongoksangami' and 'chrysanthemum' all showed an inhibitory rate of about 30% of the anti-inflammatory effect even in vivo.

Experimental Example 5: Safety Verification; Allergen Assessment>

In order to confirm allergenicity of ruby sapphire and chrysanthemum sound, LLNA (local lymph node assay) method was selected. [Reference: OECD GUIDELINE FOR THE TESTING OF CHEMICALS; DRAFT NEW GUIDELINE 429: Skin Sensitization; Local Lymph Node Assay.

The mixed extracts prepared in Preparation Examples 1 and 2 were added to 60% ethanol to prepare a 20%, 40%, and 60% solution, respectively, and 25 μl was applied to both ears of the mouse (Balb / c) for 3 days. Auricular lymph node was isolated. The isolated lymph nodes were pulverized into a single cell state, incubated for 24 hours with the addition of radioisotope [3H] -methyl thymidine, and the amplification degree [dpm] of cell proliferation was measured. The results are shown in Table 7.

sample Concentration (%) Lymph node count (cells / mL × 10 ⁷ ) ^a dpm (average) ^b SI (amplitude) ethanol 60 1.58 996 Transportation Ruby 20 1.81 1462 1.5 40 1.87 1481 1.5 60 1.28 1597 1.6 ethanol 60 1.76 1729 Transportation Chrysanthemum 20 1.57 993 0.6 40 1.73 1743 1.0 60 1.93 1122 0.6

In Table 7, adpm (disintegration per minute) is the amount of [3H] -methylthymidine inserted into the cell, bS.I. (stimulation index) is the average dpm of the sample divided by the average dpm of the control group.

As shown in Table 7, the allergy evaluation result is the lymphocyte amplification of the sample group compared to the control group. 'Hongoksangami' and 'Chrysanthemum' mixed extracts have been shown to be safe because they have an amplification degree (SI) of 3 or less and are unlikely to cause allergy.

From the above experiments, 'hongokgami' and 'chrysanthemum' mixed extracts activate cell proliferation, have excellent free radical scavenging activity, and are excellent anti-aging cosmetic compositions that inhibit prostaglandin production of cellular inflammatory reactions. , Creams, packs, gels, ointments, patches, or sprays.

As described above, the extract of 'Hongoksangami' consisting of a herbal medicine mixture of white paper, wagyang, sculpture, and clove extract of the present invention, and a 'Chrysanthemum' mixed extract consisting of a herbal mixture of chrysanthemum and licorice extract, have a cell proliferation activity and antioxidant activity in the skin. The composition having excellent efficacy, anti-inflammatory effect and synergistic effect can provide a cosmetic composition excellent in skin anti-aging effect.

Claims (10)

A cosmetic composition for preventing skin aging, comprising: a herbal mixture consisting of white paper, kwakhyang, sculptor, and clove extract; According to claim 1, wherein the weight ratio of 10:90 ~ 90:10 of 'Hakoksangami' which is a herbal mixture consisting of white paper, wagyang, sculpture and clove extract and a herbal mixture consisting of chrysanthemum and licorice extract Cosmetic composition for preventing skin aging, comprising a. The cosmetic composition for preventing skin aging according to claim 1, wherein all of the herbal mixtures are contained for the purpose of increasing cell proliferation activity. The cosmetic composition for preventing skin aging according to claim 1, wherein all of the herbal mixtures are contained for the purpose of increasing antioxidant efficacy. The cosmetic composition for preventing skin aging according to claim 1, wherein all of the herbal mixtures are contained for the purpose of increasing free radical scavenging activity.  The cosmetic composition for preventing skin aging according to claim 1, wherein all of the herbal mixtures are contained for the purpose of increasing anti-inflammatory efficacy. The cosmetic composition for preventing skin aging according to claim 1, wherein all of the herbal mixtures are contained for the purpose of inhibiting the production of prostaglandins. The anti-aging cosmetic composition according to claim 1, wherein the anti-aging cosmetic composition has a formulation of a lotion, an essence, a lotion, a cream, a pack, a gel, an ointment, a patch, or a spray. The cosmetic composition for preventing skin aging according to claim 1, wherein the herbal composition contains 0.0001 to 30% by weight based on the total weight of the skin anti-aging cosmetic composition. The extraction solvent of the herbal mixture consisting of white paper, squash, sculpture and clove extract of claim 1 and the herbal mixture consisting of chrysanthemum and licorice extract is purified water, methanol, ethanol, propyl alcohol, butyl alcohol, ethyl acetate, glycerin, propylene glycol and butylene A single pure solvent selected from the group of solvents including glycol or a mixed solvent of two or more thereof, the method of separating and extracting a cosmetically active ingredient substance, characterized in that an amount of 1 to 20 times is used with respect to the total volume of the herbal medicine.

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