KR102094846B1 - Method of mixed crude herb cosmetic composition effective for improving skin and mixed crude herb cosmetic composition thereby - Google Patents

Abstract

Disclosed are a method for manufacturing a mixed herbal medicine cosmetic composition including Schisandra chinensis, Astragalus membranaceus, Rehmannia glutinosa, and Glycyrrhiza uralensis, and a mixed herbal medicine cosmetic composition manufactured thereby. According to an embodiment of the present invention, a prepared mixed herbal medicine thermal water fermented extract including Schisandra chinensis, Astragalus membranaceus, Rehmannia glutinosa, and Glycyrrhiza uralensis exhibits excellent stability of 95% or more of cell viability, has an excellent melanin pigment synthesis inhibiting effect, has an excellent collagen synthesis promoting effect for human skin cells, and has an excellent antioxidant effect. Also, according to an embodiment of the present invention, a mixed herbal medicine cosmetic composition containing, as an active ingredient, the prepared mixed herbal medicine thermal water fermented extract including Schisandra chinensis, Astragalus membranaceus, Rehmannia glutinosa, and Glycyrrhiza uralensis causes extremely low skin irritation, has excellent skin whitening effects and anti-wrinkle effects when being used for human skin, and has excellent usability, thereby being useful for cosmetics for skin improvement and the like.

Description

Method for manufacturing a mixed herbal cosmetic composition having efficacy for improving skin and a mixed herbal cosmetic composition prepared therefrom METHOD OF MIXED CRUDE HERB COSMETIC COMPOSITION EFFECTIVE FOR IMPROVING SKIN AND MIXED CRUDE HERB COSMETIC COMPOSITION THEREBY}

The present invention relates to a method for preparing a cosmetic composition comprising a mixed herbal fermentation extract that is effective in improving skin and a mixed herbal cosmetic composition prepared therefrom.

Skin exists at the outermost part of the body and is an important organ protecting the body from the outside. All organs of all living things progress with age, and skin is no exception. In general, skin aging is divided into extrinsic aging such as intrinsic aging and photoaging. Endogenous aging means that aging occurs naturally with age, and photoaging refers to the aging phenomenon caused by sunlight.

The typical phenomena that appear as skin ages are wrinkles, blemishes, and black spots. In the case of skin wrinkles, the elasticity of collagen, which is an elastic fiber present in the dermal layer of the skin, is caused by a variety of causes, resulting in a decrease in elasticity due to decomposition or synthetic degradation. Spots such as ginseng and blotch are also caused by excessive production of melanin pigments due to hormone abnormalities or ultraviolet rays.

In the case of endogenous aging or exogenous aging, these are known to be deeply involved in free radicals. In the endogenous aging, free radicals generated in metabolic processes in living cells mainly act, and in the case of exogenous aging, the generation of free radicals by ultraviolet rays It is the main cause. Therefore, it is generally known that skin aging cannot be completely prevented, but it can delay aging to some extent by using external agents such as cosmetics containing antioxidants or collagen synthetic substances or avoiding exposure to ultraviolet rays.

Therefore, in order to make a cosmetic for delaying skin aging, it is necessary to use an antioxidant capable of promoting collagen synthesis or imparting cell regeneration or removing free radicals. Representative antioxidants include enzymes called superoxide dismutase (SOD), vitamin C, vitamin E (tocopherol), flavonoids, and the like. In addition, collagen synthesis promoting substances include vitamin C, vitamin A (retinoic acid), and skin growth factor (EGF).

When applying the above-mentioned antioxidants to cosmetics, since the compounds are unstable, it is not easy to apply to cosmetics having a long shelf life, and there is a problem that may cause side effects on the skin when used excessively. Therefore, in order to develop a cosmetic with excellent wrinkle improvement or whitening effect, it is desirable to include ingredients that are stable in the formulation and have fewer skin side effects.

In this regard, Korean Patent Publication No. 2012-0021766 discloses a liquid crystal cream containing hot spring water in the Asan region by applying an emulsifying technique using an emulsifier. However, in the preceding patent document, the contents of the fermented extract in which the extract of the mixed herbal medicine using hot spring water is fermented with a mushroom mycelium as described in the present invention are simply added at the time of cosmetic production.

Accordingly, the present inventors have repeatedly studied in order to develop cosmetics that are safe for the skin and have excellent skin whitening, wrinkle improvement and aging delay effect, and as a result, a combination of several herbal drugs among natural substances that have been traditionally used as herbal medicines It has been found that this has excellent antioxidant and collagen synthesizing powers, and the effect of the fermentation extracts of these complexes is significantly higher than that of the case of using each drug, thereby completing the present invention.

Republic of Korea Patent Publication No. 2012-0021766

The present invention has been devised to solve the above-mentioned problems, and an embodiment of the present invention provides a method for preparing a mixed herbal cosmetic composition effective in improving skin.

In addition, an embodiment of the present invention provides a mixed herbal cosmetic composition.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by a person having ordinary knowledge in the technical field to which the present invention belongs from the following description. There will be.

As a technical means for achieving the above-described technical problem, one aspect of the present invention is the step of preparing a mixed herbal by crushing Schisandra chinensis, astragalus, rhubarb and licorice, the mixed herbal is hot spring water, lower alcohol having 1 to 4 carbon atoms or these Preparing an extract by extracting with a mixed solvent of, drying the extract to prepare a mixed herbal hot spring water extract, mixing the mixed herbal hot spring extract with purified water, glucose and yeast extract to prepare a liquid medium composition, the Adding mushroom mycelium to the liquid medium composition, followed by culturing and fermenting, removing and drying the mushroom mycelium of the liquid medium composition to obtain a mixed herbal hot spring fermented extract and mixing the mixed herbal hot spring fermented extract and cosmetic formulation mixture Preparation of a mixed herbal cosmetic composition comprising the step of Provides the law.

Here, the hot spring water may be Suanbo hot spring water.

The hot spring water has a water temperature of 50 to 55 ° C, a pH of 8 to 8.5, and may include radium and sulfur.

The mushroom mycelium may be a mycelium mushroom ( Antrodia Coaphorata ).

The mushroom mycelium may be cultured in a medium containing hot spring water.

The mixed herbal may be mixed with 20 to 30 wt% of Omija, 20 to 30 wt% of astragalus, 20 to 30 wt% of sulfuric acid, and 20 to 30 wt% of licorice based on the total weight of the mixed herbal.

The weight ratio of the fermented extract of the mixed herbal hot spring water may be 1 to 10 wt% based on the weight of the mixed herbal cosmetic composition.

In addition, another aspect of the present invention provides a mixed herbal cosmetic composition containing a mixed herbal spring water fermented extract of Omija, Hwanggi, Chihwang and Licorice as an active ingredient.

The mixed herbal spring water fermented extract of Omija, Hwanggi, Chihwang and Licorice prepared according to one embodiment of the present invention exhibits excellent stability of cell survival rate of 95% or more, as shown in the toxicity measurement test for skin cells, and inhibits melanin pigment synthesis Is excellent, has an excellent effect of promoting collagen synthesis on human skin cells, and has an excellent antioxidant effect. In addition, the mixed herbal cosmetic composition containing the mixed herbal medicinal spring water fermented extract of Omija, Hwanggi, Chihwang and Licorice prepared according to one embodiment of the present invention as an active ingredient has very low skin irritation and when used on human skin , It has excellent skin whitening effect and wrinkle improvement effect, and is also excellent in use, so it can be usefully used for cosmetics for skin improvement.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a schematic diagram showing a method for preparing a mixed herbal cosmetic composition according to an aspect of the present invention.

Hereinafter, the present invention will be described in more detail. However, the present invention may be implemented in various different forms, and the present invention is not limited by the examples described herein, and the present invention is only defined by the claims below.

In addition, the terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. 'Including' a component throughout the specification of the present invention means that other components may be further included rather than excluding other components, unless otherwise stated.

One aspect of the present invention is a step of preparing a mixed herbal by crushing Schisandra chinensis, astragalus, rhubarb, and licorice, preparing the extract by extracting the mixed herbal medicine with hot spring water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, Drying the extract to prepare a mixed herbal hot water extract, mixing the mixed herbal hot water extract with purified water, glucose and yeast extract to prepare a liquid medium composition, adding the mushroom mycelium to the liquid medium composition and incubating it Method of manufacturing a mixed herbal cosmetic composition comprising the step of fermenting, removing the mushroom mycelium of the liquid medium composition and drying it to obtain a mixed herbal hot water fermented extract and mixing the mixed herbal hot water fermented extract and a cosmetic formulation mixture Gives

Hereinafter, the method for preparing the mixed herbal cosmetic composition will be described in detail for each step.

First, the method for preparing the mixed herbal cosmetic composition includes the steps of crushing Omija, Hwanggi, Chihwang and licorice to prepare a mixed herbal.

In this case, the mixed herbal may be mixed with 10-50 wt% of Omija, 10-50 wt% of astragalus, 10-50 wt% of sulfur, and 10-50 wt% of licorice based on the total weight of the mixed herbal, preferably Can be mixed with 20-30 wt% of Omija, 20-30 wt% of astragalus, 20-30 wt% of rhubarb, and 20-30 wt% of licorice.

The omija is a fruit of a vine deciduous tree belonging to the family Schizandra chinensis , which means that it is mainly used as a medicine or as a material for alcohol and tea. Omija is said to have five flavors of sweet, salty, bitter, sour, and spicy, and is said to be Omija. Among them, it has the strongest sour taste. Malic acid and tartaric acid are known as ingredients of sour taste, and are used as a medicine in oriental medicine.

The Hwanggi (黃 / 黃耆, Astragalus membranaceus) is a perennial plant distributed in Korea, China, and Japan that means an herb growing in a rock gap in a mountainous region. It is usually 40-70cm in height and is characterized by white soft hairs throughout the plant. It is commonly cultivated as a herb, and it is collected in the fall in oriental medicine to remove outcrops and roots, and dried in the sun is called the astragalus of medicinal herbs. It has effects such as tonic, cold, diuretic, and physical weakness and fatigue. It is prescribed for blood loss, uterine bleeding, intestinal sewage, cold sweat, peripheral nerve paralysis, hemiplegia, edema, chronic nephritis.

The hwang (地 黃, scientific name: Rehmannia glutinosa) means a perennial plant with the yeoldang, China is the origin. It is about 30cm high, and the leaves are oval and come out from the roots. In June-July, beautiful flowers of reddish purple bloom. The roots are usually called Jihwang, and the raw ones are called raw Jihwang, dried ones are dried Geonhwang, and steamed dried ones are called Sukjihwang. It is used as a blood medicine, tonic, and antipyretic in oriental medicine.

The licorice is a perennial herb of the legume and has an underground stem that is vine-grown and a root that extends deep into the ground. There are many leaf stems with 9 to 17 long and round leaves on the 1m long stem that extends upward. It is characterized by a three-year-old root and a stem in the ground, with moderate medicinal properties and sweet taste. The licorice is rich in vitamin C, including vitamins A and B, and is effective in acne and eczema, and also has a whitening effect and a skin soothing effect. Licorice as used herein refers to a general term used for medicinal purposes, and in particular, the roots and subterranean stems of the same plant, such as Spanish licorice (Glycyrrhiza glabra), Manchuria licorice (Glycyrrhiza uralensis), spear and licorice (Glycyrrhiza inflata), or intact. It means to remove.

The crushing method is not particularly limited, but is preferably performed in a chopped manner using a grinder.

Next, the method for preparing the mixed herbal cosmetic composition includes the step of preparing the extract by extracting the mixed herbal with hot spring water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In this case, the hot spring water has a water temperature of 50 to 55 ° C, a pH of 8 to 8.5, and may include radium and sulfur, and preferably, the hot spring water may be Suanbo hot spring water.

The water quality of Suanbo Onsen is simple, alkaline, tasteless, odorless, and very smooth. Water temperature is 53 degrees, acidity of about 8.3, weakly alkaline and contains radium, sulfur, etc., so it is effective for skin diseases, neuralgia, gynecological diseases, gastroenteritis, and fatigue recovery. The main components are chlorine 13.0-19.7 mg / L, sulfate ions 26.6-49.3 mg / L, silicon dioxide 44.3-75.1 mg / L and fluorine 5-8 mg / L, potassium 2.03-2.56 mg / L, sodium 63.8-67.8 mg / L, calcium 9.5 to 21.6 mg / L, magnesium 0.05 to 0.63 mg / L, lithium 0.16 to 0.19 mg / L, strodium 0.03 to 0.09 mg / L, manganese 0.01 to 0.02 mg / L and zinc 0.01 to 0.06 mg Include / L.

As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol, propanol or butanol may be used.

The capacity of the hot spring water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be 2 to 10 times the total weight of the mixed herbal, and preferably 4 to 6 times.

The extraction may be performed at a temperature of 5 to 100 ° C, preferably at a temperature of 20 to 30 ° C, and may be performed for 3 to 2 days, preferably for 6 to 15 hours It may be carried out, more preferably 8 to 12 hours. When the extraction time is less than 3 hours, the extraction time is too short to extract the active ingredient properly, and when the extraction time exceeds 3 days, the change in the extraction component is insufficient and the extract may be discolored.

Next, the method for preparing a mixed herbal cosmetic composition includes drying the extract to prepare a mixed herbal hot spring water extract.

At this time, the drying of the extract may be performed by a method of removing the solid content of the extract, collecting the filtrate, and then concentrating under reduced pressure, freeze drying, spray drying, cold air drying or hot air drying. It can be carried out in a drying manner.

Next, the method for preparing the mixed herbal cosmetic composition includes the step of preparing the liquid medium composition by mixing the mixed herbal hot spring water extract with purified water, glucose, and yeast extract.

In this case, the mixed herbal hot spring water extract may be mixed at 5 to 15% (w / v) based on the purified water volume. In addition, the glucose may be mixed at 2 to 4% (w / v) based on the purified water volume. Furthermore, the yeast extract may be mixed at 0.5 to 1.5% (w / v) based on the purified water volume.

The liquid medium composition may have a pH of 5 to 6, preferably 5.5.

Next, the method for preparing the mixed herbal cosmetic composition includes adding a mushroom mycelium to the liquid medium composition, followed by culturing and fermenting.

In this case, the mushroom mycelium may be a mycelium mushroom ( Antrodia Coaphorata ). It is particularly advantageous to obtain the fermented extract of hot spring water having excellent melanin synthesis inhibition, collagen synthesis promotion, and antioxidant activity from the hot spring water extract using the mycelium Antrodia Coaphorata mycelium.

Ujangji Mushroom is a rare mushroom called Jangji Mushroom, which is native to Taiwan, and parasitic on 100-year-old camphor trees native to high altitudes of 1,000 m or more above sea level. It has traditionally been used in China for hypertension, liver cancer, inflammation, itching, abdominal pain and diarrhea.

The jangjangji mushroom may be passaged every 1 to 2 months after cultivation by slope for 5 to 10 days in a yeast-wort agar medium.

In addition, the Ujangji mushroom may be cultured in a medium containing hot spring water, and the hot spring water may be Suanbo hot spring water.

The method of adding mushroom mycelium to the liquid medium composition may be performed by homogenizing the cultured mushroom mycelium and adding 1 to 10% (v / v) based on the volume of the medium composition, and the culture fermentation is rotated at a temperature of 27 ° C. It can be carried out by culturing for 5 to 10 days under conditions of water 150 rpm and aeration amount of 1.5 vvm.

Next, the method for preparing the mixed herbal cosmetic composition includes removing the mushroom mycelium of the liquid medium composition and drying it to obtain a fermented extract of the mixed herbal hot spring water.

The method for removing the mushroom mycelium may use centrifugation, and may further include obtaining a fermentation extract by filtering the culture filtrate with a filter after centrifugation. At this time, as a filter that can be used, a 0.2 to 0.5 micrometer membrane filter may be used, and the fermentation extract in the filtrate is further concentrated under reduced pressure and freeze-dried to obtain a fermented extract of hot spring water in a mixed state of dried medicine .

Finally, the method of preparing the mixed herbal cosmetic composition includes mixing the fermented extract of the mixed herbal hot water and the cosmetic formulation mixture.

At this time, the weight ratio of the mixed herbal hot water fermentation extract may be 0.01 to 30 wt% based on the total weight of the mixed herbal cosmetic composition, preferably 0.1 to 20 wt%, more preferably 1 to 10 wt% Can be

 When the weight ratio of the fermented extract of the mixed herbal hot spring water is less than 0.01 wt%, it may be difficult to provide satisfactory efficacy, and when it exceeds 30.0 wt%, it affects the odor and color of the cosmetic composition, which makes it difficult to expect additional efficacy. You can.

The formulation of the mixed herbal cosmetic composition is not particularly limited, and may be appropriately selected according to the purpose. For example, the formulation of the mixed herbal cosmetic composition may be convergent cosmetic, soft cosmetic, essence, emulsion, cream, foundation or BB cream.

In addition, the mixed herbal cosmetic composition may be applied to products for skin whitening, wrinkle improvement, antioxidant, skin moisturizing, or anti-aging purposes.

The cosmetic formulation mixture may be a component generally used in cosmetics. For example, general auxiliary ingredients such as emulsifiers, thickeners, emulsions, surfactants, lubricants, alcohols, water-soluble polymers, gelling agents, stabilizers, vitamins, inorganic salts, emulsifiers, and fragrances can be used. The ingredients may be selected within a range that does not impair the inherent effects of the mixed herbal cosmetic composition prepared according to the present invention, depending on the formulation or purpose of use. The added amount of the components may be, for example, 0.1 to 10% by weight, preferably 0.1 to 6% by weight, based on the total weight of the composition, but is not limited thereto.

Since the hot spring fermented extract of Omija, Hwanggi, Chihwang and Licorice, which are active ingredients of the mixed herbal cosmetic composition, is manufactured from natural raw materials that have been used as medicinal materials since ancient times, the mixed herbal cosmetic composition does not exhibit toxicity or side effects, and thus is a cosmetic. As can be easily used.

The mixed herbal cosmetic composition may be used daily and may also be used for an indefinite period. Preferably, according to the user's age, skin condition or skin type, and the concentration of the fermented extract, the amount of use, the number of uses, and the period can be adjusted.

In addition, an aspect of the present invention provides a mixed herbal cosmetic composition containing a mixed herbal herbal spring water ferment extract of Omija, Hwanggi, Chihwang and licorice as an active ingredient.

At this time, the composition ratio, characteristics, manufacturing method, etc. for the mixed herbal cosmetic composition are as described above.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

Example 1. Preparation of mixed herbal spring water fermentation extract according to the present invention

Step 1: Preparation of mixed herbal spring water extract

After purchasing commercially available Omija, Hwanggi, Chihwang and Licorice, mix them with 200g of Omija, 200g of Hwanggi, 200g of Jihwang and 200g of Licorice, chop them into a grinder, add 4000g of Suanbo hot spring water, and then add to it for 10 hours at 23 ℃. During extraction, an extract was prepared. The solids of the extract were removed and the filtrate was collected, then concentrated under reduced pressure and lyophilized. After drying, 20 g of dry powder was obtained as a mixed herbal spring water extract.

Step 2: Preparation of mixed herbal spring water fermentation extract

First, for use in the preparation of fermented extracts of mixed herbal spring water, yeast-wort agar medium (yeast extract 3g, wort 3g, glucose 10g, peptone 5g, agar 20g, hot spring water 1L) was added to the test tube containing jangjang mushroom mycelium for 7 days. After incubation, it was stored at 4 ° C and passaged every 1 month. The jangjangji mushroom was sold from the National Institute of Natural Resources, Jeollanam-do.

After mixing 100 mL of 10% (w / v) aqueous solution of the mixed herbal spring water extract obtained in step 1, 3 g of glucose and 1 g of yeast extract, the pH was adjusted to 5.5 and sterilized to prepare a liquid medium composition. After adding to the medium composition, 5% (v / v) by aseptically homogenizing the mycelial mushroom mycelia grown on the slope medium (containing 0.1 g as a pure mycelium), the temperature is 27 ° C, rotational speed of 150 rpm, and barrel Incubated for 7 days under the condition of 1.5 vvm, and the hot spring water extract of the mixed herbal was fermented.

After the fermentation, centrifugation was performed to remove all of the mycelium mushrooms, and the obtained filtrate was concentrated under reduced pressure and freeze-dried to obtain 14 g of mixed herbal spring water fermentation extract.

Example 2. Preparation of mixed herbal cosmetic composition according to the present invention

Fermented extract of mixed herbal spring water obtained in Example 1 based on 100 parts by weight of purified water (5 wt%), cetostearyl alcohol (3 wt%), glyceryl stearate (2 wt%), beads wax (1 wt%), polyglyceryl -3 methyl glucose distearate (1.5 wt%), squalane (5 wt%), carlylic / capric triglyceride (5 wt%), cetylethylhexanoate (5 wt%), dimethicone (0.5 wt%), Butylene glycol (5wt%), glycerin (8wt%), 1,2 hexanediol (2wt%) and ammonium acroloyldimethyl taurate / vpico polymer (0.2wt%) mixed with oil-in-water (W A mixed herbal cosmetic composition in the form of / O) cream was prepared.

Comparative Example 1-1. Preparation of Omija hot spring water extract

Commercially available omija were purchased, 800 g were taken, and chopped into grinders. After adding 4000 g of Suanbo hot spring water to Omija's crushed product, extraction was performed at 23 ° C. for 12 hours to prepare an extract. The solids of the extract were removed and the filtrate was collected, then concentrated under reduced pressure and lyophilized. After drying, 20.3 g of dry powder was obtained as an extract of Omija hot spring water.

Comparative Example 1-2. Preparation of astragalus hot spring water extract

A hot spring water extract was prepared in the same manner as Comparative Example 1-1, except that a yellow flag was used instead of Omija.

Comparative Example 1-3. Preparation of Chihwang hot spring water extract

A hot spring water extract was prepared in the same manner as in Comparative Example 1-1, except that Ojiza was used instead of Omija.

Comparative Example 1-4. Preparation of licorice hot spring water extract

A hot spring water extract was prepared in the same manner as Comparative Example 1-1, except that licorice was used instead of Omija.

Comparative Example 2-1. Preparation of Omija hot spring fermented extract

Step 1: Preparation of hot spring water extract

Commercially available omija were purchased, 800 g were taken, and chopped into grinders. After adding 4000 g of Suanbo hot spring water to Omija's crushed product, extraction was performed at 23 ° C. for 12 hours to prepare an extract. The solids of the extract were removed and the filtrate was collected, then concentrated under reduced pressure and lyophilized. After drying, 20.3 g of dry powder was obtained as an extract of Omija hot spring water.

Step 2: Preparation of hot spring fermented extract

First, incubate the mycelia mushroom mycelium for 7 days in a test tube containing yeast-wort agar medium (yeast extract 3g, wort 3g, glucose 10g, peptone 5g, agar 20g, hot water 1L) for use in the preparation of fermented extracts of hot spring water for 7 days. After that, it was stored at 4 ° C and subcultured every month. The jangjangji mushroom was sold from the National Institute of Natural Resources, Jeollanam-do.

After mixing 100 mL of 10% (w / v) aqueous solution of Omija hot spring water extract obtained in step 1, 3 g of glucose and 1 g of yeast extract, the pH was adjusted to 5.5 and sterilized to prepare a liquid medium composition. After adding to the medium composition, 5% (v / v) by aseptically homogenizing the mycelial mushroom mycelia grown on the slope medium (containing 0.1 g as a pure mycelium), the temperature is 27 ° C, rotational speed of 150 rpm, and barrel Omija hot spring water extract was fermented by culturing for 7 days under the condition of 1.5 vvm.

After the fermentation, centrifugation was performed to remove all the mycelium mushrooms, and the obtained filtrate was concentrated under reduced pressure and freeze-dried to obtain 15.6 g of Omija hot spring water fermentation extract.

Comparative Example 2-2. Preparation of Hwanggi hot spring water fermentation extract

A fermented extract of hot spring water was prepared in the same manner as Comparative Example 2-1, except that a yellow flag was used instead of Omija.

Comparative Example 2-3. Preparation of fermented hot spring water fermentation extract

A fermented extract of hot spring water was prepared in the same manner as Comparative Example 2-1, except that Ojiza was used instead of Omija.

Comparative Example 2-4. Preparation of licorice hot spring fermented extract

A fermented extract of hot spring water was prepared in the same manner as Comparative Example 2-1, except that licorice was used instead of Omija.

Comparative Example 3. Preparation of fermented extract of mixed herbal spring water

14.3 g of mixed herbal spring water fermentation extract was prepared in the same manner as in Example 1, except that the mixed herbal extract was extracted together with ginseng.

Comparative Example 4. Preparation of mixed herbal spring water extract

After mixing and crushing 200 g of Omija, 200 g of astragalus, 200 g of turmeric, and 200 g of licorice, 4000 g of Suanbo hot spring water was added, and then extracted at 23 ° C. for 10 hours. After the extraction was completed, the solids were removed, the filtrate was collected, and then concentrated under reduced pressure and lyophilized to obtain 19.3 g of hot spring water extract of a mixed herbal.

Comparative Example 5. Preparation of Hwanggi's fermented extract of hot spring water

14.5 g of fermented extract of hot spring water of astragalus was obtained in the same manner as in Example 1, except that 800 g of astragalus was mixed instead of the mixed herbal.

Comparative Example 6. Preparation of cosmetic composition 1

A cosmetic composition was prepared without adding a mixed herbal hot spring water fermentation extract.

Fragrance (suitable amount), cetostearyl alcohol (3wt%), glyceryl stearate (2wt%), beads wax (1wt%), polyglyceryl-3 methyl glucose distearate (1.5wt%) based on 100 parts by weight of purified water ), Squalane (5 wt%), carlylic / capric triglyceride (5 wt%), cetyl ethylhexanoate (5 wt%), dimethicone (0.5 wt%), butylene glycol (5 wt%), glycerin ( 8wt%), 1,2 Hexanediol (2wt%) and ammonium acroloyldimethyl taurate / vpico polymer (0.2wt%) to prepare a cosmetic composition in the form of an oil-in-water (W / O) cream. It was prepared.

Comparative Example 7-1. Preparation of cosmetic composition 2

Omija hot spring water extract (5wt%), cetostearyl alcohol (3wt%), glyceryl stearate (2wt%), beads wax (1wt%), polyglyceryl obtained in Comparative Example 1-1 based on 100 parts by weight of purified water -3 methyl glucose distearate (1.5 wt%), squalane (5 wt%), carlylic / capric triglyceride (5 wt%), cetylethylhexanoate (5 wt%), dimethicone (0.5 wt%), Butylene glycol (5wt%), glycerin (8wt%), 1,2 hexanediol (2wt%) and ammonium acroloyldimethyl taurate / vpico polymer (0.2wt%) mixed with oil-in-water (W A cosmetic composition in the form of / O) cream was prepared.

Comparative Example 7-2. Preparation of cosmetic composition 3

A cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the yellowish hot spring water extract (5 wt%) obtained in Comparative Example 1-2 was used instead of the Omija hot spring water extract (5 wt%) obtained in Comparative Example 1-1. Did.

Comparative Example 7-3. Preparation of cosmetic composition 4

A cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that instead of the Omija hot spring water extract (5 wt%) obtained in Comparative Example 1-1, the Jihwang hot spring water extract (5 wt%) obtained in Comparative Example 1-3 was used. Did.

Comparative Example 7-4. Preparation of cosmetic compositions 5

A cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the licorice hot spring water extract (5wt%) obtained in Comparative Example 1-3 was used instead of the Omija hot spring water extract (5wt%) obtained in Comparative Example 1-1. Did.

Comparative Example 8-1. Preparation of cosmetic composition 6

A cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the Omija hot spring fermented extract obtained in Comparative Example 2-1 (5wt%) was used instead of the Omija hot spring extract obtained in Comparative Example 1-1 (5wt%). It was prepared.

Comparative Example 8-2. Preparation of cosmetic compositions 7

The cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the fermented extract of the aerated hot spring water obtained in Comparative Example 2-2 (5 wt%) was used instead of the Omija spring water extract obtained in Comparative Example 1-1 (5 wt%). It was prepared.

Comparative Example 8-3. Preparation of cosmetic compositions 8

The cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the fermented hot spring water fermentation extract (5 wt%) obtained in Comparative Example 2-3 was used instead of the Omija hot spring water extract (5 wt%) obtained in Comparative Example 1-1. It was prepared.

Comparative Example 8-4. Preparation of cosmetic compositions 9

The cosmetic composition was prepared in the same manner as in Comparative Example 7-1, except that the licorice hot spring fermented extract obtained in Comparative Example 2-4 (5wt%) was used instead of the Omija hot spring extract obtained in Comparative Example 1-1 (5wt%). It was prepared.

Comparative Example 9. Preparation of cosmetic composition 10

Based on 100 parts by weight of purified water, Omija hot spring fermented extract obtained in Comparative Example 2-1 (1.25wt%), astragalus hot spring water fermented extract obtained in Comparative Example 2-2 (1.25wt%), obtained in Comparative Example 2-3 Fermented hot spring water fermentation extract (1.25wt%), licorice spring water fermentation extract obtained in Comparative Example 2-4 (1.25wt%), cetostearyl alcohol (3wt%), glyceryl stearate (2wt%), beads wax (1wt %), Polyglyceryl-3 methyl glucose distearate (1.5 wt%), squalane (5 wt%), carlylic / capric triglyceride (5 wt%), cetylethylhexanoate (5 wt%), dimethicone (0.5wt%), butylene glycol (5wt%), glycerin (8wt%), 1,2 hexanediol (2wt%) and ammonium acroloyldimethyl taurate / vpico polymer (0.2wt%) By mixing, an oil-in-water (W / O) type cream-like cosmetic composition was prepared.

Table 1 below shows the components and yields of the extracts prepared in Example 1 and Comparative Examples 1-1 to 2-4.

Classification extract Yield (g) Example 1 Mixed herbal spring water fermentation extract 14 Comparative Example 1-1 Omija hot spring water extract 20.3 Comparative Example 1-2  Astragalus hot spring water extract 19.9 Comparative Example 1-3  Chihwang Hot Water Extract 20.5 Comparative Example 1-4  Licorice hot spring water extract 19.5 Comparative Example 2-1 Omija hot spring fermented extract 15.6 Comparative Example 2-2 Fermented extract of astragalus hot spring water 14.7 Comparative Example 2-3 Fermented hot spring water extract 14.4 Comparative Example 2-4 Licorice hot spring fermented extract 14.1

In Table 2, the components and contents of the cosmetic compositions prepared in Example 2 and Comparative Examples 6 to 9 are summarized.

ingredient Content (wt%) Example 2 Comparative Example 6 Comparative Example 7-1 Comparative Example 7-2 Comparative Example 7-3 Comparative Example 7-4 Comparative Example 8-1 Comparative Example 8-2 Comparative Example 8-3 Comparative Example 8-4 Comparative Example 9 Cetostearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl stearate 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Beads wax 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polyglyceryl-3 methyl glucose distearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Caprylic / Capric Triglyceride 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Cetyl ethyl hexanoate 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Purified water 100 100 100 100 100 100 100 100 100 100 100 Butylene Glycol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 glycerin 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 1,2 hexanediol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Ammonium acroloyl dimethyl taurate / VP copolymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Spices - Proper - - - - - - - - - Fermented extract of hot spring water of mixed herbal medicine
(Example 1) 5.0 - - - - - - - - - - Omija hot spring water extract
(Comparative Example 1-1) - - 5.0 - - - - - - - - Astragalus hot spring water extract
(Comparative Example 1-2) - - - 5.0 - - - - - - - Chihwang Hot Water Extract
(Comparative Example 1-3) - - - 5.0 - - - - - - - Licorice hot spring water extract
(Comparative Example 1-4) - - - - - 5.0 - - - - - Omija hot spring water fermentation extract
(Comparative Example 2-1) - - - - - - 5.0 - - - 1.25 Fermented extract of Hwanggi hot spring water (Comparative Example 3) - - - - - - - 5.0 - - 1.25 Fermented hot spring water extract (Comparative Example 4) - - - - - - - - 5.0 - 1.25 Licorice hot spring fermented extract (Comparative Example 5) - - - - - - - - - 5.0 1.25

Experimental Example 1. Skin cell toxicity measurement experiment of extract

Each of the hot spring water extracts prepared in Example 1 and Comparative Examples 1-1 to Comparative Examples 1-4 or fermented spring water extracts was tested to measure skin cell toxicity.

Distilled water was used as a diluent, and each extract was prepared at concentrations of 100 ppm, 150 ppm, and 200 ppm, and then treated with skin tissue to measure cell viability.

The cell line used for the experiment was three types of cells: human dermal fibroblasts (Lonza, Basel, Switzerland). The cells were Dulbecco's Modified Eagle Medium (DMEM; Gibco-BRL, Invitrogen; Life Technologies, Gaithersburg, MD, USA) with 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin / Streptomycin (penicillin 100 IU / mL, streptomycin 100μg / mL; Life Technologies), using a medium containing 5% CO ₂ was cultured in a cell culture under a temperature of 37 ℃.

The WST-1 (Water-soluble tetrazolium salt) assay is a method of measuring and determining the activity of mitochondrial NADH-dehydrogenase in a cell to treat tetrazolium salt in living cells. When the colored formazan is formed by the dehydrogenase in the mitochondria, the formed formazan is checked for absorbance at a wavelength of 450 nm to check the concentration of living cells.

The cells were divided into 2 × 10 ³ cells / well cells in a 96-well plate, cultured for 24 hours, and then treated with the appropriate concentration to incubate for 24 hours. After that, the WST-1 assay solution was treated at 10% of the medium amount for 0.5 to 1 hour at 37 ° C, and then absorbed at 450 nm using an iMark microplate reader (Bio-Rad, Hercules, CA, USA). Was measured and the reference absorbance was measured at 650 nm to correct the result. Results were expressed as mean ± standard deviation from three independent experiments. P <0.05 was verified through Student's t-test, and indicates the significance of the results.

Table 3 below shows the concentration and cell viability for each Example and Comparative Example.

Sample name Concentration (ppm) Cell viability (%)
Example 1 Fermented extract of mixed herbal spring water 100 97.2 150 96.8 200 95.5
Comparative Example 1-1
Omija hot spring water extract 100 97.6 150 99.7 200 99.3
Comparative Example 1-2
Astragalus hot spring water extract 100 97.4 150 96.5 200 95.3
Comparative Example 1-3
Chihwang Hot Water Extract 100 97.8 150 97.7 200 98.6
Comparative Example 1-4
Licorice hot spring water extract 100 95.4 150 95.3 200 93.5

As can be seen through Table 3, it can be confirmed that the hot spring water fermented extract of the mixed herbal according to the present invention is a safe component that does not cause toxicity to skin cells.

Experimental Example 2. Experiment of measuring the inhibitory effect of melanin synthesis of extract

After dispensing B16F10 cells into 3 × 10 ⁵ cells in a 60 mm culture dish, samples of Example 1 and Comparative Examples 1-1 to 2-4 were treated with 200 μg / ml concentration in the medium. For positive control, α-MSH (Sigma-Aldrich), a hormone that promotes melanin synthesis, was treated in the same cells at a concentration of 200 nM. After the sample and α-MSH were treated with B16F10 melanoma cells, cells were incubated at room temperature for 48 hours, cells were harvested, washed twice with PBS, and melanin synthesis inhibition was measured for each sample.

Sample name Melanin synthesis inhibition rate (%) Example 1 Fermented extract of mixed herbal spring water 65.4 Comparative Example 1-1 Omija hot spring water extract 23.5 Comparative Example 1-2 Astragalus hot spring water extract 20.3 Comparative Example 1-3 Chihwang Hot Water Extract 18.7 Comparative Example 1-4 Licorice hot spring water extract 14.7 Comparative Example 2-1 Omija hot spring water fermentation extract 28.6 Comparative Example 2-2 Fermented extract of Hwanggi hot spring water 22.3 Comparative Example 2-3 Fermented hot spring water extract 20.5 Comparative Example 2-4 Licorice Hot Water Fermentation Extract 17.5

As can be seen from Table 4, in the melanin synthesis inhibition rate, the mixed herbal medicinal spring water fermented extract of Omija, astragalus, rhizome and licorice corresponding to Example 1 is different from the hot spring water extract of Comparative Examples 1-1 to 2-4, or It can be seen that it is significantly superior to the fermented extract of hot spring water.

Experimental Example 3. Measurement of collagen synthesis promoting effect on human skin cells

Collagen and collagenase (collagenase) expression was measured by using the qRT-PCR (quantitative real-time PCR) analysis to measure the amount of mRNA of the corresponding representative gene. The amount of collagen was confirmed by analyzing the expression level of COL1A1 gene, a representative component of type 1 collagen, and collagenase was confirmed by analyzing the expression level of MMP1 gene that has type 1 collagen decomposition. Human dermal fibroblasts (Human dermal fibroblasts, Lonza, Basel, Switzerland) were dispensed into 5 × 10 ⁵ cells in a 60-mm culture dish and cultured for 24 hours. Thereafter, samples of Example 1 and Comparative Examples 1-1 to 2-4 were added at a concentration of 200 μg / ml to incubate for 24 hours. After harvesting the cultured cells, 1 ml of TRIzol reagent (Life Technology) was added to perform cell lysis and total mRNA extraction. The concentration and purity (A260 / A280 and A260 / A230 ratio) of Total mRNA was verified using a MaestroNano ^® microvolume spectrophotometer (Maestrogen, Las Vegas, NV, USA), and only total mRNA with a purity higher than 2.0 was selected. Total mRNA was replaced with cDNA using M-MLV reverse transcriptase (Life Technologies, USA), and used for qRT-PCR. qRT-PCR was performed using HOT FIREPol EvaGreen PCR Mix Plus (Solis BioDyne, Estonia), and the expression of the gene was analyzed using Line-Gene K software (Bioer Technology Co. Ltd., Hangzhou, China).

PCR reaction conditions were denaturation at 94 ° C for 5 minutes, followed by denaturation (94 ° C, 30 seconds), annealing (60 ° C, 30 seconds), polymerization (72 ° C, 30 seconds) for 40 cycles. Changes in gene expression were measured in comparison to the expression level of the β-ACTIN control gene. Results were expressed as mean ± standard deviation from three independent experiments. P <0.05 was verified through Student's t-test, and indicates the significance of the results.

Sample name Collagen biosynthesis increase rate (%) Example 1 Fermented extract of mixed herbal spring water 75.1 Comparative Example 1-1 Omija hot spring water extract 28.3 Comparative Example 1-2 Astragalus hot spring water extract 25.8 Comparative Example 1-3 Chihwang Hot Water Extract 23.2 Comparative Example 1-4 Licorice hot spring water extract 20.4 Comparative Example 2-1 Omija hot spring water fermentation extract 33.5 Comparative Example 2-2 Fermented extract of Hwanggi hot spring water 28.1 Comparative Example 2-3 Fermented hot spring water extract 25.3 Comparative Example 2-4 Licorice Hot Water Fermentation Extract 22.7

As can be seen from Table 5, the collagen biosynthesis efficacy of Example 1 was remarkably superior to Comparative Examples 1-1 to 2-4.

Experimental Example 4. Measurement of the antioxidant effect of the extract

To measure the antioxidant activity of Example 1 and Comparative Examples 1-1 to 2-4, DCF-DA (2'7'-Dichlorofluorescein diacetate) assay was performed. This is a test method used for the purpose of evaluating the ability to remove free radicals by comparing the amount of free radicals generated in cells stimulated with silica after treatment with the test substance. First, the raw 264.7 cells cultured for 24 hours were washed once with PBS, and then the cells were harvested. After the cells were suspended with 15 mL PBS, 15 μl of 20 mM DCF-DA solution (Sigma-Aldrich) was added and reacted for 1 hour. After centrifugation at 1200 × g for 2 minutes, washed once with PBS, diluted with 1 × 10 ⁵ cells / mL, and then treated with the sample at a concentration of 200 μg / ml and reacted for 10 minutes. Thereafter, 50 μl of 20 mg / mL silica was further reacted for 1 hour. The cell pellet obtained by centrifugation at 6000 × g for 5 minutes was dispersed in 200 μl PBS and inoculated into a 96-well plate. Then, the fluorescence microplate reader (Bio-Rad) was used to measure the fluorescence at 485 nm excitation / 535 nm emission to determine the ROS level. Results were expressed as mean ± standard deviation from three independent experiments. P <0.05 was verified through Student's t-test, and indicates the significance of the results.

Sample name Antioxidant effect (free radical scavenging rate,%) Example 1 Fermented extract of mixed herbal spring water 65.1 Comparative Example 1-1 Omija hot spring water extract 20.3 Comparative Example 1-2 Astragalus hot spring water extract 15.6 Comparative Example 1-3 Chihwang Hot Water Extract 18.3 Comparative Example 1-4 Licorice hot spring water extract 15.4 Comparative Example 2-1 Omija hot spring water fermentation extract 23.5 Comparative Example 2-2 Fermented extract of Hwanggi hot spring water 17.3 Comparative Example 2-3 Fermented hot spring water extract 20.1 Comparative Example 2-4 Licorice Hot Water Fermentation Extract 17.1

As can be seen from Table 6, the antioxidant activity of Example 1 was significantly superior to Comparative Examples 1-1 to 2-4.

Experimental Example 5. Skin irritation test of cosmetic composition

In order to compare the degree of skin irritation for the cosmetic compositions of Examples 2 and 6 to 8-4, a closed patch test was performed on the human body. For 20 healthy male and female subjects, 0.2 g of each sample was applied to the inside of the lower abdomen, the pin chamber was removed after 24 hours, and after 4 hours, the skin condition was visually checked. Skin irritation was calculated using Equation 1 below.

[Equation 1]

Sample name Number of subjects Judgment result Irritation - +- + ++ Example 2 20 people 20 0 - - 0.00 Comparative Example 6 19 One - - 0.02 Comparative Example 7-1 19 One - - 0.02 Comparative Example 7-2 20 0 - - 0.00 Comparative Example 7-3 19 One - - 0.02 Comparative Example 7-4 20 0 - - 0.00 Comparative Example 8-1 19 One - - 0.02 Comparative Example 8-2 20 0 0.00 Comparative Example 8-3 20 0 0.00 Comparative Example 8-4 20 0 0.00 -: No erythema or peculiarities
+-: Slightly redder than the surroundings
+: Significantly reddening than the surroundings
++: Redden and swell more severely than the surroundings

As can be seen from Table 7, the cosmetic composition containing the fermented extract of hot spring water of a mixed herbal was confirmed to have very low skin irritation.

Experimental Example 6. Skin elasticity test of cosmetic composition

For the cosmetic composition of Example 2, Comparative Example 6 and Comparative Example 9, the skin elasticity change was measured before and after 8 weeks of use after using it twice a day in the morning and evening in order to investigate the effect of improving skin elasticity. The skin elasticity was measured using a skin elasticity meter (Cutometer SEM 575), and the temperature was measured at 25 ° C and 75% humidity. For Example 2, Comparative Example 6 and Comparative Example 9, 60 healthy women aged 30-55 were divided into three groups of 20 each, and the results are shown in Table 8 below.

sample Skin elasticity increase rate (%) Example 2 23.5 Comparative Example 6 13.6 Comparative Example 9 15.3

As can be seen from Table 8, it can be seen that the cosmetic composition of Example 2 containing the mixed herbal fermentation extract has a significantly higher skin elasticity increase rate than the cosmetic compositions of Comparative Examples 6 and 9.

Experimental Example 7. Human usability test of cosmetic composition

For the cosmetic compositions of Examples 2, 6, and 9, in order to investigate the skin whitening effect and wrinkle improvement effect in actual use, 69 healthy 30-55 year old adult women, respectively, Example 2, Comparative Example 6 and Divided into 3 groups of Comparative Example 9, a human usability test was conducted. 50 g of the test articles prepared with the composition of Example 2, Comparative Example 6 and Comparative Example 9 were distributed together with the questionnaire, used twice a day in the morning and evening for a total of 8 weeks, and then the questionnaire was collected and analyzed.

Among the total of 69 subjects, 22 of the Example 2 group, 21 of each of the Comparative Examples 6 and 9 group, and a total of 64 questionnaires were collected and analyzed, excluding the dropout (3) and poor questionnaire (2). It is shown in Table 9 below.

Respondents Very worse Slightly worse change
none Slightly better Very good Skin color change Example 2 - - 8 10 4 Comparative Example 6 - One 17 3 - Comparative Example 9 - - 14 7 - Skin elasticity change degree Example 2 - - 7 10 5 Comparative Example 6 - One 16 4 - Comparative Example 9 - - 14 7 - Skin wrinkle change degree Example 2 - - 8 9 5 Comparative Example 6 - - 16 5 - Comparative Example 9 - - 15 6 -

As can be seen from Table 9, the cosmetic composition of Example 2 containing the mixed herbal fermentation extract, compared to the cosmetic composition of Comparative Example 6 or Comparative Example 9, the user for skin color, skin elasticity, skin wrinkle improvement efficacy You can see that the satisfaction level is high.

Claims (8)

Preparing a mixed herbal by pulverizing 20-30 wt% of Omija, 20-30 wt% of astragalus, 20-30 wt% of sulfuric acid and 20-30 wt% of licorice;
Preparing the extract by extracting the mixed herbal with hot spring water, wherein the water temperature is 50-55 ° C, the pH is 8-8.5, and contains radium and sulfur;
Drying the extract to prepare a mixed herbal hot spring water extract;
Preparing a liquid medium composition by mixing the mixed herbal hot spring water extract with purified water, glucose and yeast extract;
Adding fermented jangjang mushroom mycelium to the liquid medium composition, followed by culturing to ferment;
Removing the mushroom mycelium of the liquid medium composition and drying it to obtain a fermented extract of mixed herbal hot spring water; And
Mixing the mixed herbal hot spring water fermentation extract and cosmetic formulation mixture
Method for producing a mixed herbal cosmetic composition comprising a.
According to claim 1,
The hot spring water is a method of manufacturing a mixed herbal cosmetic composition that is Suanbo hot spring water.
delete delete According to claim 1,
The jangjangji mushroom mycelium is a method of manufacturing a mixed herbal cosmetic composition, characterized in that cultured in a medium containing hot water.
delete According to claim 1,
The weight ratio of the fermented extract of the mixed herbal hot spring water is 1 to 10 wt% based on the weight of the mixed herbal cosmetic composition.
A mixed herbal cosmetic composition comprising a mixed herbal medicinal spring water fermented extract of Omija, Hwanggi, Chihwang and Licorice as an active ingredient, prepared according to the method of claim 1.

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