KR20130052288A - Cosmetic composition comprising the hot spring water extract of nelumbo nucifera gaertner as active ingredient - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a phreatic water extract of lotus as an active ingredient is provided to ensure excellent antioxidation, whitening, anti-wrinkling, and astringent effects. CONSTITUTION: A cosmetic composition contains 0.3-3 parts by weight of a phreatic water extract of lotus as an active ingredient. The extract is prepared from lotus flower, leaves, or stems. The cosmetic composition is used for antioxidation, whitening, anti-wrinkling, and astringent effects. The cosmetic composition is manufactured in a formulation selected from a group consisting of soap, a lotion, cream, a pack, and a cosmetic toner.

Description

Cosmetic composition comprising the spring water extract of lotus as an active ingredient {Cosmetic composition comprising the hot spring water extract of Nelumbo nucifera gaertner as active ingredient}

The present invention relates to a cosmetic composition comprising an extract of lotus hot spring water having an antioxidant effect, a whitening effect, a skin wrinkle improvement effect, and an astringent effect as an active ingredient.

With the recent advent of an aging society and the spread of well-being trends, there is an increasing demand for maintaining health and beauty for a long time. Interest in materials is increasing.

Natural plant materials have been used for a long time in the cosmetics industry because they are safer and more environmentally friendly than synthetic materials. Recently, herbal cosmetics using herbal medicine with strong physiological activity has become the mainstream of cosmetic products. Korean traditional cosmetics have been used for hundreds of years and have been proven in terms of safety and effectiveness, so they can be easily recognized and used by consumers, and the effects are inferior to synthetic materials.

Natural plant material or herbal medicine contains various biologically active substances and antioxidants, and has various effects such as anti-aging, anticancer, anti-inflammatory, and immune function improvement.

There are many substances that have excellent physiological activity on the skin and have anti-aging and whitening effects. Retinol, arbutin, vitamin C, etc. are known to be effective for wrinkle improvement and whitening as typical functional materials. However, most of these attempts to find a material that replaces it by causing instability, ineffectiveness, or side effects in the formulation are an essential part of cosmetic material research. Recently, attempts have been made to search for substances with excellent physiological activity in natural plant materials, not synthetic ones, and plants and herbal medicines showing useful effects on skin, such as anti-aging and whitening, are found one after another.

On the other hand, lotus root, stem, leaf, fruit, etc., various parts of the plant has been used for a long time as a food or herbal medicine, the safety of which has been proved significantly, and the price is also relatively inexpensive at about 2,000 won per kg, which is an ideal environment for cosmetic materials. Yeongeon has about 550 hectares of cultivated area in Korea. The major production areas are Haman, Chilseo, Hanlim, Harbin, Daegu Banyeol, Jeonnam, and Gyeongseong. In the herbal medicine, the kite relieves the lack of energy, especially the heart, rain, and the lack of energy of the gods, and the deterioration of the stomach. 12 The blood of the meridians is greatly increased, and the rice and the meat are soaked to make it light and strong. It is said to blacken and to long life. Lotus flower is mainly used for ornamental purposes, but its use has not been studied much. Especially, the leaf contains alkaloid and tannin, which is used as astringent and hemostatic agent or in the folk medicine to treat enuresis. The underground stem is called lotus root, it contains asparagine, it is edible and it is used as a nourishing medicine. Fruit is fed, and seed is called soft meat, and is known to be good for women's diseases.

Korean Patent Publication No. 10-2011-0115047

The present inventors have measured the antioxidant effect, the whitening effect, the skin wrinkle improvement effect, the astringent effect of the lotus extract, and as a result, the efficacy was found to be effective as a cosmetic composition.

The present invention is to provide a cosmetic composition comprising an extract of lotus hot spring water as an active ingredient.

The present invention relates to a cosmetic composition comprising an extract of lotus hot spring water having an antioxidant effect, a whitening effect, a skin wrinkle improvement effect, and an astringent effect.

Cosmetic composition of the present invention containing a lotus extract as an active ingredient has excellent antioxidant, whitening, skin wrinkle improvement and skin astringent effect, so it is suitable as a functional component of cosmetics for improving skin function, the effect is also excellent.

Figure 1 relates to the total coliform group test results of the three hot spring water.
2 is a graph of the electron donating ability of the lotus extract.
Figure 3 is a graph of the SOD-like activity of the lotus extract.
Figure 4 is a graph of xanthine oxidase inhibitory activity of the lotus extract.
5 is a graph of tyrosinase inhibitory activity of the lotus extract.
Figure 6 is a graph of the inhibitory activity against elastase of the lotus extract.
7 is a graph showing the inhibitory activity against collagenase of lotus extract.
8 is a graph showing a comparison of astringent activity of lotus extract.

The present invention relates to a cosmetic composition containing a lead extract as an active ingredient.

The kite may be a flower, a leaf, or a stem of the kite, but is not limited thereto.

The lotus extract may be a spring water extract, any method for obtaining an effective extract from the lotus can be used in the scope of the present invention.

The hot spring water may be, but is not limited to, Dogo hot spring water, Asan hot spring water, or hot spring hot spring water, and in particular, Asan hot spring.

The Dogo Hot Spring is a cold mineral spring whose water temperature is maintained at 35 degrees. The water quality is simple sulfur spring and is one of the four major sulfur hot springs in the Orient. The main component is hydrogen ion concentration of 8.7%, and other solid residues, silicic acid, sodium, calcium, magnesium and other components. Neuralgia, skin disease, gastrointestinal disease, arthritis, arteriosclerosis, chronic bronchitis, constipation, traumatic sequelae, rheumatism, women's diseases, diabetes, genital diseases, digestive diseases, skin beauty is effective.

The Asan hot spring is an alkaline hot spring containing sodium bicarbonate, which contains a large amount of 20 beneficial ingredients for the human body and is surrounded by dense forest and is a versatile hot spring that can serve as a forest bath. It is effective in promoting blood circulation, promoting cell regeneration, neuralgia, arthritis, high blood pressure, gastrointestinal diseases, wind and skin beauty.

The hot spring water is geologically composed of single-phase biotite, hornblende granite, the temperature of the eluted hot spring water is a hot spring temperature of about 58 ℃. Its main ingredient is radium hot spring containing manitaon. It is slightly alkaline and is effective in skin care, angiosclerosis, neuralgia, gynecology and anemia.

In Figure 1 it can be seen that the total coliform group is not detected in the three hot spring water is suitable as an extraction solvent. When using hot spring water as an extraction solvent, it may have the advantage of obtaining a lead extract containing components such as sulfur, sodium bicarbonate, and manitaone, and the pH of the hot spring water may be suitable as an extraction solvent. Table 1 relates to the pH, moisturizing power, and sulfur content analysis of the three hot spring waters.

spa pH Moisturizing Sulfur content Distilled water
(Cosmetic additives) pH 6.3 5 X Dogo Hot Spring pH 7.5 7 O (trace detection) Asan Hot Spring pH 7.3 6 X Onyang Hot Spring Water pH 8.2 6 X

As a result of Table 1, in the case of distilled water 5 as a reference point, Dogo hot water was the best moisturizing power, and sulfur was detected in trace amount in Dogo hot water.

In one embodiment of the present invention, the lotus extract may be contained in 0.3 to 3 parts by weight based on 100 parts by weight of the total cosmetic composition. If there is less lead extract than the above range, the effect is insignificant, in many cases it is difficult to expect a variety of effects according to another composition component added to the composition.

That is, the cosmetic composition according to the present invention may contain other ingredients and the like which can give a synergistic effect to the main effect within the range that does not impair the main effect by the active ingredient, for example, fragrances, In addition to additives such as pigments, fungicides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, synthetic polymers, and the like, as well as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extracts It may further comprise an auxiliary component. The above ingredients may be suitably selected and blended by those skilled in the art according to the dosage form or purpose of use, and the amount thereof may be selected within a range that does not impair the purpose and effect of the present invention, but based on 100 parts by weight of the total cosmetic composition 0.01 to 5 parts by weight, may be 0.01 to 3 parts by weight.

In addition, the cosmetic composition comprising a lotus extract as an active ingredient is a cosmetic composition that can be used for the purpose of antioxidant, whitening, wrinkle improvement, skin convergence.

More specifically, in one embodiment of the present invention was measured the antioxidant efficacy of the cosmetic composition of the present invention. In particular, in relation to the measurement of antioxidant efficacy, the electron donating ability, SOD-like activity, and xanthine oxidase inhibitory activity were measured.

2,2-Diphenyl-1-picrylhydrazyl (DPPH), which is used for the measurement of electron donating ability, is a relatively stable free radical and is measured by antioxidant and aromatic amines to measure antioxidant activity using the principle of dark purple color fading. The electron donating ability measurement method is a method that is widely used because it is easy to measure the antioxidant activity of the plant extract and also highly related to the actual antioxidant activity. As a result of measuring the electron donating ability using the lotus extract of the present invention, the high electron donating ability was confirmed in the order of extracting the spring water of lotus leaf, lotus flower, and lotus root.

Superoxide dismutase (SOD) is an antioxidant enzyme that catalyzes the conversion of oxygen species that are harmful to cells into hydrogen peroxide. SOD-like substances are not enzymes but are small molecule substances that play a role similar to SOD. It belongs mainly to phytochemical and protects the living body from superoxide. In addition, SOD is a protein having a relatively high molecular weight and is weak in heat or alkali and is a low-molecular substance that can compensate for such shortcomings. As a result of measuring the SOD-like activity using the lotus extract of the present invention, the SOD-like activity was confirmed in the order of extract of spring water of lotus root, lotus and lotus leaf.

Xanthine oxidase is an enzyme that is involved in purine metabolism in vivo and forms uric acid from xanthine or hypoxanthine, and when uric acid is increased in plasma, it accumulates in the skeleton due to low solubility, causing gout that causes severe pain. Xanthine oxidase also catalyzes the reaction of oxidizing xanthine to uric acid using oxygen as a hydrogen acceptor. Therefore, the inhibitory effect of xanthine oxidase inhibits the production of free radicals, and thus has biological significance, such as antioxidant, aging and anticancer. As a result of measuring the xanthine oxidase inhibitory activity using the lotus extract of the present invention, it was confirmed that xanthine oxidase inhibitory activity in the order of the spring water extract of lotus root, lotus leaf, lotus flower.

In addition, in one embodiment of the present invention, the whitening effect of the cosmetic composition of the present invention was measured by tyrosinase inhibitory activity. When skin aging is promoted, the free radicals produced are known as the causative factors for promoting mutagenesis, carcinogenesis including skin cancer, adult diseases and aging. Tyrosinase is an enzyme that contains copper and is responsible for the transition from L-tyrosine to L-DOPA and DOPA to L-dopaquinone, which are the early stages of melanin synthesis. Ultraviolet mitosis of melanocytes occurs, followed by activation of melanocytes. In activated melanocytes, tyrosinase synthesis is promoted and melanin production is promoted to be transported out of the epidermis, resulting in pigmentation such as blemishes and freckles. As a result of measuring tyrosinase inhibitory activity using the lotus extract of the present invention, the tyrosinase inhibitory activity was confirmed in the order of extract of lotus leaf, lotus root, and hot spring water of lotus flower.

In addition, in one embodiment of the present invention, the antiwrinkle effect of the cosmetic composition of the present invention was measured by the elastase inhibitory activity and collagenase inhibitory activity. Elastase, which degrades elastin, which is present in the dermis and plays an important role in maintaining the elasticity of the skin, is increased with age and due to harmful environment such as ultraviolet rays or free radicals. Provides an important cause for formation. Elastase is an enzyme that activates external stimuli such as UV and causes wrinkles on the face by breaking down fibrous proteins such as keratin. It is a nonspecific hydrolase that can break down collagen, another important substrate protein. As a result of measuring the elastase inhibitory activity using the lotus extract of the present invention it was confirmed that the elastase inhibitory activity in the order of the spring water extract of lotus, lotus root, lotus leaf.

In addition, collagen, which is a major component of the extracellular matrix, is a major substrate protein produced in fibroblasts of the skin. The main functions of collagen are known as mechanical firmness of skin, resistance of connective tissue and binding of tissue, support of cell adhesion, induction of cell division and differentiation. Such collagen is reduced by age and photoaging by ultraviolet irradiation, which is known to be closely related to wrinkle formation of the skin. As a result of measuring the collagenase inhibitory activity using the lotus extract of the present invention, it was confirmed that the collagenase inhibitory activity in the order of the spring water extract of lotus leaf, lotus, lotus root.

In addition, in one embodiment of the present invention, the astringent activity of the cosmetic composition of the present invention was measured by astringent activity. Skin proteins bind to polyphenols, which are high molecular flavonoids, form cross-links, and the skin contracts to converge. Convergence basically means to wrinkle or shrink. Loss of elasticity and pores, such as the pores of the enlarged skin astringent action gives the tension of the skin and at the same time can give elasticity. In this experiment, the degree of convergence effect was determined according to the degree of binding of hemoglobin protein to the extract. This astringent action has the effect of forming a poorly soluble coating on the surface of the skin and mucous membranes externally or internally, thereby protecting the local or densifying tissues, thereby reducing the permeability of the cell membrane. Polyphenolic compounds such as tannins are secondary metabolic products of plants widely distributed in nature and have various physiological activities. In addition, polyphenolic compounds are characterized by forming strong complexes with proteins, certain types of polysaccharides and carbohydrates, nucleic acids, and alkaloids. The astringent action in the skin is characterized by the interaction of these phenolic compounds with skin proteins. Phenolic OH groups attract proteins by interacting with the surface of the protein and acting as linkers to link the proteins. This interaction allows the skin to feel the contraction, and sebum secretion is reduced. The interaction between phenolic compounds and proteins also binds to proteolytic enzymes and hyaluronic acid hydrolases in the skin, thereby inhibiting the action of these enzymes and inhibiting skin protein damage. As a result of measuring the collagenase inhibitory activity using the lotus extract of the present invention, it was confirmed that the collagenase inhibitory activity in the order of the spring water extract of lotus, lotus root, lotus leaf.

The cosmetic composition according to the present invention may be one formulation selected from the group consisting of soaps, lotions, creams, packs, and cosmetic liquids. That is, the composition according to the present invention may be included in soap, lotion, cream, pack and cosmetic liquid in the form of external skin preparations and applied to the skin. However, this is only one example, and cosmetics of any formulation including the cosmetic composition according to the present invention are within the scope of the present invention, and the present invention is not limited to the types of the formulations listed above. For example, it may be administered in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, or the like, in a variety of ways such as simple drinking, injection, spray or squeeze.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

< Example  1> Preparation of lotus extract

Extraction of the sample was performed by adding 10 times the amount of Asan hot water to 100 g of each part of the flower, leaf, and stem of the lotus, reflux-cooling extraction at 85 ° C. for 3 hours, and extracting the supernatant and the precipitate three times. The extracts were centrifuged, filtered, concentrated and freeze-dried and stored in the freezer as samples of this experiment.

< Example  2>-antioxidant effect

1.Measuring method

(One) Electron donating ability  Measure

Electron donating abilities (EDA) were measured by modifying Blois' method. For DPPH radical scavenging activity, 120 μl of sample solution for each concentration was added, 60 μl of 0.2mM DPPH solution (sigma aldrich) was added, and the absorbance was measured at 517 nm using a 96-well microplate reader after 15 minutes of reaction at room temperature. The electron donating ability was expressed as the absorbance reduction rate of the sample solution added and the sample free.

(2) Superoxide Dismutase ( Superoxide dismutase , SOD ) Similar activity  Measure

SOD-like activity was measured by modifying Marklund's method. The similar activity of superoxide dismutase (SOD) was mixed with 20µl of sample solution, 130µl of 50mM Tris-HCl buffer (pH8.5) and 20µl of 7.2mM pyrogallol (sigma, USA), followed by mixing at 37 ℃ for 10 minutes. After the reaction, the amount of oxidized pyrogallol was measured at an absorbance of 420 nm using a microplate reader. SOD-like activity was expressed by the absorbance reduction rate of the sample solution added and no added solution.

(3) Xanthine  Oxidase ( Xanthine oxidase ) Inhibitory activity measurement

Xanthine oxidase inhibitory activity was measured by modifying the method of Stirpe and corte. Xanthine oxidase inhibitory activity was measured in 10 μl of sample solution by concentration in 40 μl of 0.1M Potassium phosphate buffer solution (pH8.0) and 100 μl of Xanthine (0.4mM xanthine + 0.24mM Nitro blue tetrazolium) (sigma, USA) and Xanthine oxidase (sigma). USA) 100 μl was added and reacted at 37 ° C. for 20 minutes. The uric acid produced in the reaction solution was measured at an absorbance of 500 nm using a microplate reader. Xanthine oxidase inhibitory activity was indicated by the decrease in absorbance of the sample solution added and non-added.

2. Measurement result

(One) Electron donating ability  Confirmation result

The results of measuring the electron donating ability of the lotus leaf, flower, root extract are shown in FIG. The hot water extracts of lotus leaf, flower, and root were high electron donating ability of 79.2%, 72.0%, 59.3% at 500ppm, respectively, and the high electron donating ability of hot water extract of lotus leaf, flower, and root was confirmed.

(2) SOD Pseudo-activity  Verification result

The results of measuring the SOD-like activity of the lotus leaf, flower, and root extract are shown in FIG. 3. Hot spring extracts of lotus leaf, flower and root showed 20.6%, 27.1% and 42.4% of activity at 1000ppm, respectively. Spring water extracts of lotus leaf, flower, and root were found to be SOD-like activity, although somewhat lower.

(3) Xanthine  Results of confirming oxidase inhibitory activity

As a result of measuring the Xanthine oxidase inhibitory activity of the lotus leaf, flower, root extract is shown in FIG. The spring water extracts of lotus leaf, flower and root extracts showed 29.1%, 20.3% and 32.6% activity at 1000ppm, respectively. The extracts of lotus leaf, flower and root showed Xanthine oxidase inhibitory activity.

< Example  3>-whitening effect

1. Measurement method

(One) Tyrosinase ( tyrosinase ) Inhibitory activity measurement

Tyrosinase inhibitory activity was measured according to the method of Yagi et al. 40 µl of mushroom tyrosinase (200U / mL) (sigma, USA) was added to a mixture of 40 µl of 10 mM L-DOPA (sigma, USA) dissolved in 80 µl of 67 mM sodium phosphate buffer (pH 6.8) and 40 µl of sample solution. Was added and reacted at 37 ° C. for 10 minutes to measure DOPA chrome produced in the reaction solution at 492 nm. Tyrosinase inhibitory activity was shown by the absorbance reduction rate of the added and non-treated samples.

2. Measurement result

(One) Tyrosinase  Inhibitory activity confirmation result

In this experiment, the inhibitory effect of tyrosinase enzyme activity on the concentration of lotus leaf, flower and root extracts was measured using Mushroom tyrosinase enzyme. As shown in Fig. 5, the extracts of lotus leaf, flower and root were 44.9%, 32.3%, at 1000 ppm, respectively. Inhibitory effect was 44.3%. Although somewhat low, Tyrosinase's inhibitory effect was observed in hot spring extracts of lotus leaf, flowers and roots.

< Example  4>-Wrinkle improvement effect

1.Measuring method

(One) Elastase  Measurement of inhibitory activity

Elastase inhibitory activity was measured according to Cannell et al. Using N-succinyl- (L-Ala) 3-p-nitroanilide (sigma, U.S.A) as the substrate, the amount of p-nitroanilide produced from the substrate was measured at 445 nm for 30 minutes at 37 ° C. That is, each test solution was prepared to a certain concentration, 40 μl each was taken on a 96-well plate, and 40 μl of porcine pancreas elastase (2.5 U / mL) (sigma, USA) solution dissolved in 50 mM tris-HCl buffer (? 8.6). After the addition of N-succinyl- (L-Ala) 3-p-nitroanilide (0.5mg / ml) dissolved in 50mM tris-HCl buffer (pH8.6) as a substrate was measured by reacting for 30 minutes. Elastase inhibitory activity was expressed as the absorbance decrease rate of the sample solution added and the no added solution.

(2) Collagenase ( collagenase ) Inhibitory activity measurement

Collagenase inhibitory activity was measured according to the method of W? Nsch E, Heindrich HG. In other words, 4 mM CaCl2 was added to 0.1M tris-HCl buffer (? 7.5) to dissolve 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg / mL) (sigma, USA). 0.15 ml of collagenase (0.2 mg / ml) (sigma, USA) was added to a mixed solution of 0.25 ml and 0.1 ml of sample solution. The mixture was left at room temperature for 20 minutes, and then 0.5 ml of 6% citric acid was added to stop the reaction. 1.5 ml was added and the absorbance was measured at 320 nm. Collagenase inhibitory activity was shown by the absorbance decrease rate of the addition and no addition of sample solution.

2. Measurement result

(One) Elastase  Inhibitory Activity Measurement Results

As a result of measuring the elastase inhibitory activity associated with wrinkle formation is shown as FIG. The hot spring water extracts of lotus leaf, flower and root showed inhibitory effect of 30.2%, 60.3% and 32.5% at 1000ppm, respectively, and the hot spring water extract of lotus flower had higher Elastase inhibitory activity than lotus leaf and lotus root extract.

(2) Collagenase ( collagenase ) Inhibitory activity measurement result

Collagenase inhibitory activity of lotus leaf, flower, and root extract was measured as shown in FIG. 7. Hot spring extracts of lotus leaf, flower and root showed 97.1%, 76.8% and 46.9% activity at 1000ppm, respectively. Among all the extracts, the hot spring water extract of lotus leaf was the most effective, followed by the hot spring water extract of lotus flower and the hot spring water extract of lotus root.

< Example  5>-Convergence Effect

1.Measurement Method Astringent  Active measurement

Astringent activity was measured according to Lee et al. Using blood protein (hemoglobin) similar to skin protein, each sample solution and hemoglobin (sigma, USA) solution were mixed 1: 1 in a centrifuge tube container, followed by shaking mixing, followed by centrifugation at 1,500 rpm for 3 minutes at 576 nm. Absorbance was measured. Astringent activity was measured by the absorbance reduction rate of the sample solution addition group and no addition group.

2.Measurement result Convergent  Active measurement result

Experimental results of the convergence effect are shown in FIG. 8. The hot spring water extracts of lotus leaf, flower and root showed 15.1%, 91.2% and 66.2% activity at 5000ppm, respectively.

Claims (6)

Cosmetic composition containing as an active ingredient of lotus hot spring water extract. The cosmetic composition according to claim 1, which is a flower, a leaf or a stem of the lotus. According to claim 1, wherein the lotus extract is a cosmetic composition, characterized in that the spring water extract. The cosmetic composition according to claim 1, wherein the lotus extract is contained in an amount of 0.3 to 3 parts by weight based on 100 parts by weight of the total cosmetic composition as an active ingredient. The cosmetic composition according to claim 1, wherein the cosmetic composition is an antioxidant, whitening, skin wrinkle improvement, astringent cosmetic composition. The cosmetic composition according to any one of claims 1 to 5, wherein the cosmetic composition is one formulation selected from the group consisting of soap, lotion, cream, pack, and essence.

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